Journal of Trace Elements in Medicine and Biology最新文献

{"title":"Is chromium(III) pharmacologically relevant? An update focused on studies with diabetic rodent models","authors":"John B. Vincent","doi":"10.1016/j.jtemb.2024.127453","DOIUrl":"https://doi.org/10.1016/j.jtemb.2024.127453","url":null,"abstract":"<div><p>A decade ago, the author assessed the status of chromium as the trivalent ion as an essential element and as a therapeutic agent based on rodent studies for this journal. The current review was undertaken to update considerations regarding the status of chromium, focusing on studies of Cr supplementation of diabetic rodent models over the last decade. Cr can no longer be considered an essential trace element for humans. Observed effects of Cr on rodent models of insulin resistance and diabetes are best interpreted in terms of a pharmacological role for Cr. The review of studies on the effects of Cr on rat models of diabetes is updated, and the results continue to suggest Cr increases insulin sensitivity in peripheral tissues of the rodent models. The lack of effects in human studies may stem from humans receiving a comparably smaller dose than the rodent models. However, given the different responses to Cr in the rodent models, humans could potentially have different responses to Cr. Recent studies primary utilizing rodents suggest two potential complementary but also contradictory modes of action for Cr(III) at a molecular level.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140631408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction and competition for intestinal absorption by zinc, iron, copper, and manganese at the intestinal mucus layer","authors":"Vincent Einhorn ,&nbsp;Hajo Haase ,&nbsp;Maria Maares","doi":"10.1016/j.jtemb.2024.127459","DOIUrl":"10.1016/j.jtemb.2024.127459","url":null,"abstract":"<div><p>Trace elements such as zinc, manganese, copper, or iron are essential for a wide range of physiological functions. It is therefore crucial to ensure an adequate supply of these elements to the body. Many previous investigations have dealt with the role of transport proteins, in particular their selectivity for, and competition between, different ions. Another so far less well investigated major factor influencing the absorption of trace elements seems to be the intestinal mucus layer. This gel-like substance covers the entire gastrointestinal tract and its physiochemical properties can be mainly assigned to the glycoproteins it contains, so-called mucins. Interaction with mucins has already been demonstrated for some metals. However, knowledge about the impact on the respective bioavailability and competition between those metals is still sketchy. This review therefore aims to summarize the findings and knowledge gaps about potential effects regarding the interaction between gastrointestinal mucins and the trace elements iron, zinc, manganese, and copper. Mucins play an indispensable role in the absorption of these trace elements in the neutral to slightly alkaline environment of the intestine, by keeping them in a soluble form that can be absorbed by enterocytes. Furthermore, the studies so far indicate that the competition between these trace elements for uptake already starts at the intestinal mucus layer, yet further research is required to completely understand this interaction.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0946672X24000798/pdfft?md5=cd23cb99d62f0fcb7ba9306bc3d55ce2&pid=1-s2.0-S0946672X24000798-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140613509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inflammatory immunity and bacteriological perspectives: A new direction for copper treatment of sepsis","authors":"Zhenzhen Huang ,&nbsp;Lunfei Cao ,&nbsp;Dengfeng Yan","doi":"10.1016/j.jtemb.2024.127456","DOIUrl":"10.1016/j.jtemb.2024.127456","url":null,"abstract":"<div><p>Copper is an essential trace element for all aerobic organisms because of its unique biological functions. In recent years, researchers have discovered that copper can induce cell death through various regulatory mechanisms, thereby inducing inflammation. Efforts have also been made to alter the chemical structure of copper to achieve either anticancer or anti-inflammatory effects. The copper ion can exhibit bactericidal effects by interfering with the integrity of the cell membrane and promoting oxidative stress. Sepsis is a systemic inflammatory response caused by infection. Some studies have revealed that copper is involved in the pathophysiological process of sepsis and is closely related to its prognosis. During the infection of sepsis, the body may enhance the antimicrobial effect by increasing the release of copper. However, to avoid copper poisoning, all organisms have evolved copper resistance genes. Therefore, further analysis of the complex relationship between copper and bacteria may provide new ideas and research directions for the treatment of sepsis.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0946672X24000762/pdfft?md5=462c2b7f72207f5b110fa8284e610e33&pid=1-s2.0-S0946672X24000762-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140758747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of the linalool against cadmium-induced testicular tissue damage","authors":"Tuba Yalçın ,&nbsp;Sercan Kaya","doi":"10.1016/j.jtemb.2024.127455","DOIUrl":"https://doi.org/10.1016/j.jtemb.2024.127455","url":null,"abstract":"<div><p>Cadmium (Cd) is a heavy metal that has harmful effects and is one of the contaminants found in the environment. Cd exposure causes important pathophysiological processes, such as reproductive toxicity. Linalool (Lnl) is a monoterpene, a component of essential oils known to be produced synthetically. Additionally, Lnl has many important beneficial effects, such as anti-inflammatory and antioxidant effects. The objective of this study is to determine whether Lnl has a healing impact in opposition to testicular tissue damage due to Cd exposure. In the study, 28 male rats were divided at random into four equal groups (n = 7). No treatment was applied to the control group. CdCl₂ was applied intraperitoneally to the Cd group at a dose of 3 mg/kg for the first 7 days of the trial. For the Cd + Lnl group, 3 mg/kg CdCl₂ was applied intraperitoneally for the first 7 days of the trial, and 100 mg/kg/day Lnl was applied. Upon completion of all applications, the rats were sacrificed and blood samples and testicular tissue were taken. Cd exposure caused histopathological changes, oxidative stress, inflammation, and an increase in apoptotic cells in testicular tissue. However, Cd altered endocrine hormones in the hypothalamic-pituitary-gonad axis. However, Lnl application against Cd exposure was able to regulate the negativity caused by Cd in both testicular tissue and endocrine hormone levels. In conclusion, Lnl may be a potential therapeutic strategy against Cd-induced reproductive toxicity. We believe that Lnl has a high potential for further studies to determine its detailed mechanisms of action and cellular signaling pathways.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140639036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of carcinogenic and non-carcinogenic risk of exposure to potentially toxic elements in tea infusions: Determination by ICP-OES and multivariate statistical data analysis","authors":"Mahmood Ahmed ,&nbsp;Muhammad Ahmad ,&nbsp;Muhammad Ayyan Khan ,&nbsp;Aamir Sohail ,&nbsp;Mudassar Sanaullah ,&nbsp;Waqar Ahmad ,&nbsp;Dure Najaf Iqbal ,&nbsp;Khuram Khalid ,&nbsp;Tanveer A. Wani ,&nbsp;Seema Zargar","doi":"10.1016/j.jtemb.2024.127454","DOIUrl":"https://doi.org/10.1016/j.jtemb.2024.127454","url":null,"abstract":"<div><h3>Background</h3><p>The perennial evergreen tea (<em>Camellia sinensis</em>) plant is one of the most popular nonalcoholic drinks in the world. Fertilizers and industrial, agricultural, and municipal activities are the usual drivers of soil contamination, contaminating tea plants with potentially toxic elements (PTEs). These elements might potentially accumulate to larger amounts in the leaves of plants after being taken up from the soil. Thus, frequent monitoring of these elements is critically important.</p></div><div><h3>Methods</h3><p>The present study intended to determine PTEs (Al, Cr, Mn, Fe, Co, Ni, Cu, Zn, Cd, and Pb) in both tea leaves and infusions using ICP-OES. Various multivariate data analysis methods such as principal component analysis (PCA) and hierarchical cluster analysis (HCA) were employed to elucidate the potential sources of PTEs contamination, whether from anthropogenic activities or natural origins. Additionally, Pearson's correlation coefficient (PCC) was calculated to assess the relationships between the variables under study.</p></div><div><h3>Results</h3><p>The mean contents (mg/L) of all studied elements in tea infusions decreased in order Mn (150.59 ± 1.66) &gt; Fe (11.39 ± 0.99) &gt; Zn (6.62 ± 0.89) &gt; Cu (5.86 ± 0.62) &gt; Co (3.25 ± 0.64) &gt; Ni (1.69 ± 0.23) &gt; Pb (1.08 ± 0.16) &gt; Cr (0.57 ± 0.09) &gt; Cd (0.46 ± 0.09) &gt; Al (0.05 ± 0.008), indicating that Mn exhibits the highest abundance. The mean concentration trend in tea leaf samples mirrored that of infusions, albeit with higher concentrations of PTEs in the former. The tolerable dietary intake (TDI) value for Ni and provisional tolerable monthly intake (PTMI) value for Cd surpassed the standards set by the WHO and EFSA. Calculated hazard index (HI &lt; 1) and cumulative cancer risk (CCR) values suggest negligible exposure risk.</p></div><div><h3>Conclusion</h3><p>Elevated levels of PTEs in commonly consumed tea products concern the public and regulatory agencies.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140643488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of cancer risk and radiological effects from 210Po and 210Pb with consumption of wild medicinal herbal plants","authors":"Aleksandra Moniakowska,&nbsp;Dagmara Strumińska-Parulska","doi":"10.1016/j.jtemb.2024.127452","DOIUrl":"https://doi.org/10.1016/j.jtemb.2024.127452","url":null,"abstract":"<div><h3>Background</h3><p>Plants' raw materials can accumulate chemical elements, including radioisotopes, to some extent and reflect the region they grow in. Due to their high environmental content and toxicity, natural radionuclides – polonium ²¹⁰Po and lead ²¹⁰Pb are the most dangerous radioisotopes for human health.</p></div><div><h3>Methods</h3><p>70 raw medicinal herbal materials from 54 popular wild-growing medicinal plant species, most of them included in the European Pharmacopoeia, were collected across three regions. In 210 samples, the activity concentrations of ²¹⁰Po and ²¹⁰Pb were determined, and their radiotoxicity and cancer risk were calculated.</p></div><div><h3>Results</h3><p>The results of ²¹⁰Po and ²¹⁰Pb activities measured in herbal raw material were from 0.12 mBq g⁻¹ dry weight in the fruits of the common chestnut (<em>Aesculus hippocastanum</em>) to 19.7 mBq g⁻¹ dw in herb of ground elder (<em>Aegopodium podagraria</em>) for ²¹⁰Po, while for ²¹⁰Pb ranged from 0.16 mBq g⁻¹ dw to 34.3 mBq g⁻¹ dw in the same plants. There were statistically significant differences between ²¹⁰Po and ²¹⁰Pb content in leaves, herbs, flowers, and fruit. The studies indicated that the estimated annual effective radiation dose from Polish medicinal herbs consumption was low, ranging 0.03–105 µSv from ²¹⁰Po and 0.02–98.2 µSv from ²¹⁰Pb, while the risk of cancer morbidity and mortality ranged from 10⁻⁵ to 10⁻⁹.</p></div><div><h3>Conclusions</h3><p>Consumption of the tested medicinal herbs is safe from the radiological point of view and, based on the predictions related to the calculated risk of cancer, should not increase the probability of its occurrence.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0946672X24000725/pdfft?md5=24a17f2f85b8e7297686a7a6494735c8&pid=1-s2.0-S0946672X24000725-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140553971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of imprecision in the different detection methods of Zn based on 5 years of data from an external quality assessment program in China","authors":"Chao Zhang ,&nbsp;Ying Yan ,&nbsp;Chuanbao Zhang","doi":"10.1016/j.jtemb.2024.127451","DOIUrl":"https://doi.org/10.1016/j.jtemb.2024.127451","url":null,"abstract":"<div><h3>Background</h3><p>This study examines the imprecision of zinc (Zn) measurements across various clinical detection methods by analyzing the external quality assessment (EQA) data from 2018 to 2022. The findings of this study aim to offer recommendations for enhancing Zn measurements.</p></div><div><h3>Methods</h3><p>Participating laboratories were grouped into peer categories based on the detection methods. The robust mean and coefficient of variation (CV) of the samples were calculated following ISO 13528 guidelines. The evaluation criteria for optimal, desirable, and minimum allowable imprecision in Zn estimation are 2.50<!--> <!-->%, 5.05<!--> <!-->%, and 7.55<!--> <!-->%, respectively, based on biological variation. Furthermore, the study examined inter-lab CVs, inter-method bias, and the passing rate. The impact of sample concentration on CVs and the pass rate was also investigated.</p></div><div><h3>Results</h3><p>Over the past five years, 4283 laboratories participated in the EQA program, showing a high pass rate that improved as sample concentration increased. Differential pulse polarography (DPP) demonstrated stable and low CVs (0.61–1.86<!--> <!-->%). Although differential pulse stripping (DPS) was less stable than DPP, it still exhibited a low CV (0.71–3.10<!--> <!-->%). Graphite furnace atomic absorption spectrometry (GFAAS) and flame atomic absorption spectrometry (FAAS) performed similarly and displayed stable CVs (2.39–4.42<!--> <!-->%) within the acceptable range of desirable imprecision (5.05<!--> <!-->%). However, the CVs for ICP-MS were unacceptable in three out of the five years (5.28–6.20<!--> <!-->%). In 2022, the number of participating laboratories for DDP, DPS, GFAAS, FAAS and ICP-MS is 131, 35, 35, 820 and 72, respectively.</p></div><div><h3>Conclusion</h3><p>This study provides reliable insights into the imprecision of Zn measurements in clinical laboratories. The findings indicate that additional efforts are required to reduce the imprecision of ICP-MS in Zn measurements.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140605670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concentrations and influencing factors of 17 elements in placenta, cord blood, and maternal blood of women from an e-waste recycling area","authors":"Yacui Luo ,&nbsp;Haijun Zhang ,&nbsp;Fangzhong Gui ,&nbsp;Jiayang Fang ,&nbsp;Haijiang Lin ,&nbsp;Danhong Qiu ,&nbsp;Lingfei Ge ,&nbsp;Qiong Wang ,&nbsp;Peiwei Xu ,&nbsp;Jun Tang","doi":"10.1016/j.jtemb.2024.127449","DOIUrl":"10.1016/j.jtemb.2024.127449","url":null,"abstract":"<div><h3>Background</h3><p>The effects of prenatal element exposure on mothers and fetuses have generated concern. Profiles of trace and toxic elements in biological material are urgently desired, especially for women who reside near e-waste recycling facilities. The aim of this study was to investigate elements concentrations in placenta, cord blood, and maternal blood of women and to evaluate the influencing factors.</p></div><div><h3>Methods</h3><p>A group of 48 women from an e-waste recycling site and a group of 31 women from a non-e-waste recycling site were recruited. Basic characteristics were collected by questionnaire and the concentrations of 17 elements in placenta, cord blood, and maternal blood samples were analyzed by inductively coupled plasma mass spectrometry (ICP-MS). Finally, the generalized linear model regression analysis (GLM) was used to test the association between element concentrations and possible factors.</p></div><div><h3>Results</h3><p>Compared to the control group, the exposed group had significantly elevated cadmium (Cd), zinc (Zn), nickel (Ni), and antimony (Sb) in placenta, and higher lead (Pb) in maternal blood and cord blood (P&lt;0.05). Sb concentration in maternal blood was significantly lower than in the control group (<em>P</em>&lt;0.05). GLM analysis showed that element concentrations were mainly associated with maternal age [chromium (Cr), iron (Fe), selenium (Se), cobalt (Co), mercury (Hg) in placenta, copper (Cu) in maternal blood], education (Se, Sb in placenta), family income (Cu in maternal blood and Ni in placenta), passive smoking [Cu and Zn in placenta, Pb in maternal blood], and e-waste contact history (Hg in cord blood, Cu, Zn, and Cd in maternal blood).</p></div><div><h3>Conclusions</h3><p>Women in the e-waste recycling area had higher toxic element levels in the placenta and blood samples. More preventive measures were needed to reduce the risk of element exposure for mothers and fetuses in these areas.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140613797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Zinc on hemorheological parameters in a rat model of diabetes","authors":"Nural Pastacı Özsobacı ,&nbsp;Denizhan Karış ,&nbsp;Alev Meltem Ercan ,&nbsp;Derviş Özçelik","doi":"10.1016/j.jtemb.2024.127450","DOIUrl":"https://doi.org/10.1016/j.jtemb.2024.127450","url":null,"abstract":"<div><p>Diabetes mellitus (DM) is a complex, chronic metabolic disorder characterized by impaired regulation of blood glucose levels. Zinc (Zn) is an essential trace elements that plays a role in various physiological processes within the body, including those related to diabetes. The current study was investigated the effect of Zn supplementation on hemorheological parameters in a rat model of DM. After induction of DM, 32 male Wistar albino rats were divided into four groups: control, Zn, DM, and Zn+DM. Whole blood viscosity (WBV) was determined by using digital cone and plate viscometer and plasma viscosity (PV) was determined by a Coulter Harkness capillary viscometer. The rats in the DM Group showed a decrease in both Zn levels and body weight, as well as an increase in glucose levels when compared to the control group. Diabetic rats supplemented with Zn displayed lower blood glucose levels and higher concentrations of Zn compared to the DM Group. The higher PV and lower hematocrit level were measured in DM Group than control group and lower PV, higher hematocrit level were measured in Zn+DM group than DM Group. The WBV was measured at four different shear rates (57.6–115.2 - 172.8–230.4 s ⁻¹). A statistically significant increase was observed in the DM group compared to the control group. Additionally, a statistically significant decrease was observed in the Zn+DM Group compared to the DM Group at a shear rate of 230.4 s⁻¹. Erythrocyte rigidity index (T_k) and oxygen delivery index (ODI) were computed under conditions of high shear rate. The rats in the DM group exhibited a reduction in ODI and an elevation in T_k in comparison to the control group. Conversely, the diabetic rats supplemented with Zn exhibited decreased T_k and increased ODI compared to the DM Group. Zn supplementation seems to have a potential beneficial effect for protecting adverse affect of diabetes on hemorheogical parameters and for maintaining vascular health.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140621068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterizations of biogenic selenium nanoparticles and their anti-biofilm potential against Streptococcus mutans ATCC 25175","authors":"Binbin Si ,&nbsp;Yang Yang ,&nbsp;Muhammad Naveed ,&nbsp;Fenghuan Wang ,&nbsp;Malik Wajid Hussain Chan","doi":"10.1016/j.jtemb.2024.127448","DOIUrl":"https://doi.org/10.1016/j.jtemb.2024.127448","url":null,"abstract":"<div><h3>Introduction</h3><p><em>S. mutans</em> has been identified as the primary pathogenic bacterium in biofilm-mediated dental caries. The biogenic selenium nanoparticles (SeNPs) produced by <em>L. plantarum</em> KNF-5 were used in this study against <em>S. mutans</em> ATCC 25175.</p></div><div><h3>Objectives</h3><p>The aims of this study were: <strong>(1)</strong> the biosynthesis of SeNPs by <em>L. plantarum</em> KNF-5, <strong>(2)</strong> the characterization of SeNPs, <strong>(3)</strong> the investigation of the inhibitory effect of biogenic SeNPs against <em>S. mutans</em> ATCC 25175, and (<strong>4)</strong> the determination of the anti-biofilm potential of SeNPS against <em>S. mutans</em> ATCC 25175.</p></div><div><h3>Methodology</h3><p>3 mL of the culture was added to 100 mL of MRS medium and incubated. After 4 h, Na₂SeO₃ solution (concentration 100 μg/mL) was added and incubated at 37 °C for 36 h. The color of the culture solution changed from brownish-yellow to reddish, indicating the formation of SeNPs. The characterization of SeNPs was confirmed by UV-Vis spectrophotometry, FTIR, SEM-EDS and a particle size analyzer. The antibacterial activity was determined by the disk diffusion method, the MIC by the micro-double dilution method, and the biofilm inhibitory potential by the crystal violet method and the MTT assay. The effect of SeNPs on <em>S. mutans</em> ATCC 25175 was determined using SEM and CLSM spectrometry techniques. The sulfate-anthrone method was used to analyze the effect of SeNPs on insoluble extracellular polysaccharides. The expression of genes in <em>S. mutans</em> ATCC 25175 was analyzed by real-time quantitative polymerase chain reaction (RT-qPCR).</p></div><div><h3>Preparation of nanoparticles</h3><p>SeNPs produced by probiotic bacteria are considered a safe method. In this study, <em>L. plantarum</em> KNF-5 (probiotic strain) was used for the production of SeNPs.</p></div><div><h3>Results</h3><p>The biogenic SeNPs were spherical and coated with proteins and polysaccharides and had a diameter of about 270 nm. The MIC of the SeNPs against <em>S. mutans</em> ATCC 25175 was 3.125 mg/mL. Biofilm growth was also significantly suppressed at this concentration. The expression of genes responsible for biofilm formation (GtfB, GtfC, BrpA and GbpB,) was reduced when <em>S. mutans</em> ATCC 25175 was treated with SeNPs.</p></div><div><h3>Conclusion</h3><p>It was concluded that the biogenic SeNPs produced by <em>L. plantarum</em> KNF-5 was highly effective to inhibit the growth of <em>S. mutans</em> ATCC 25175.</p></div><div><h3>Novelty statement</h3><p>The application of biogenic SeNPs, a natural anti-biofilm agent against <em>S. mutans</em> ATCC 25175. In the future, this study will provide a new option for the prevention and treatment of dental caries.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140553970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}