Journal of Trace Elements in Medicine and Biology最新文献

{"title":"Association of urinary cadmium with renal injury biomarkers and optimal cut-off value of urinary cadmium in preschool children from a mining area of northwestern China","authors":"Gulipiyan Balajiang ,&nbsp;Yue Du ,&nbsp;Wenzheng Yuan ,&nbsp;Jingru Xie ,&nbsp;Wenting Zhao ,&nbsp;Ting Yu ,&nbsp;Shiwei Ai ,&nbsp;Yuhui Dang","doi":"10.1016/j.jtemb.2025.127689","DOIUrl":"10.1016/j.jtemb.2025.127689","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to explore the relationship between urinary cadmium (U-Cd) and biomarkers of renal injury, identify sensitive biomarkers associated with cadmium-exposure-related renal injury, and evaluate the optimal cut-off value for U-Cd in preschool children.</div></div><div><h3>Methods</h3><div>Morning urine samples were collected from 420 preschool children (231 boys and 189 girls) to detect urinalysis, U-Cd, and renal injury biomarkers, including urinary N-acetyl-β-D-glucosaminidase (UNAG), urinary β₂-microglobulin (Uβ₂-MG), and urinary retinol-binding protein (URBP). Pearson correlation, quantile regression, and logistic regression models were utilized to explore the relationships between U-Cd and the renal injury biomarkers. Receiver operating characteristic curves were employed to determine the optimal cut-off value of U-Cd for inducing abnormalities in renal injury biomarkers.</div></div><div><h3>Results</h3><div>U-Cd demonstrated positive associations with UNAG, Uβ₂-MG, and URBP. The optimal cut-off values of U-Cd for inducing abnormalities in UNAG, Uβ₂-MG, URBP, and combined biomarker were 7.78, 14.74, 12.75, and 10.42 μg/g cr, respectively. When the sensitivity was set at 95 %, the cut-off values were adjusted to 4.70, 10.42, 11.07, and 5.18 μg/g cr, respectively.</div></div><div><h3>Conclusion</h3><div>U-Cd was significantly associated with renal injury biomarkers. Our findings suggest that the appropriate cut-off value for U-Cd should be established based on the sensitivity and specificity of various renal injury biomarkers.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"90 ","pages":"Article 127689"},"PeriodicalIF":3.6,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144491287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Echinochrome guarding effect against sodium arsenite-induced hepatorenal toxicity in rats","authors":"Hader I. Sakr ,&nbsp;Tarek Atia ,&nbsp;Neamat A. Mahmoud ,&nbsp;Islam Ahmed Abdelmawgood ,&nbsp;Marina Lotfy Khalaf ,&nbsp;Bassam Waleed Ebeed ,&nbsp;Ahmed Mohamed Abdelmohsen ,&nbsp;Mohamed A. Kotb ,&nbsp;Abdeljalil Mohamed Al Shawoush ,&nbsp;Ahmed A. Damanhory ,&nbsp;Abdallah Mohammed Elagali ,&nbsp;Ayman Saber Mohamed ,&nbsp;Hadeer Hesham Abdelfattah","doi":"10.1016/j.jtemb.2025.127682","DOIUrl":"10.1016/j.jtemb.2025.127682","url":null,"abstract":"<div><h3>Background</h3><div>The persistent and accumulative qualities of the poisonous metalloid arsenic make it a ubiquitous environmental threat. Echinochrome (Ech) is a natural product that possesses antioxidative, antiviral, antialgal, anti-allergic, and antibacterial effects.</div></div><div><h3>Aim</h3><div>The work investigates the beneficial impact of Ech on sodium arsenite-induced hepatorenal toxicity in rats. Eighteen male rats dispersed equally among three groups: control, sodium arsenite (AS), and AS + Ech. Rats were administered AS (10 mg/kg) and Ech (1 mg/kg BW) by gavage for the experimental duration of 30 days. Ech inhibits oxidative stress by improving antioxidant levels, including glutathione, catalase, and glutathione-S-transferase, with a concomitant decrease in the amounts of malondialdehyde and nitric oxide in kidney and liver tissues. Moreover, it reduced blood concentrations of aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltransferase, alkaline phosphatase, serum urea, uric acid, and creatinine. Concurrently, Ech resulted in a substantial increase in albumin and total protein levels. Additionally, Ech inhibits inflammation by reducing serum concentrations of tumor necrosis factor-α, cyclooxygenase 2, and prostaglandin E2.</div></div><div><h3>Conclusion</h3><div>Ech mitigates arsenic-induced hepatorenal damage by inhibiting oxidative stress and inflammatory pathways.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"90 ","pages":"Article 127682"},"PeriodicalIF":3.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Four new bright members of the ZinPyr zinc fluorescence sensor family for live cell imaging","authors":"Marisa F. Jakobs ,&nbsp;Annika M. Pick ,&nbsp;Max Carlsson ,&nbsp;Simon Wittmann ,&nbsp;Jörg Fahrer ,&nbsp;Sabine Becker","doi":"10.1016/j.jtemb.2025.127683","DOIUrl":"10.1016/j.jtemb.2025.127683","url":null,"abstract":"<div><div>As the second most abundant trace element, zinc plays numerous roles in the human body. Not only tightly bound as structural component and co-factor of more than 3000 proteins, but also as labile bound zinc, so-called mobile Zn (<em>mZn)</em>. This <em>mZn</em> occurs especially in the central nervous system, where it plays a fundamental role in signal transduction. Accordingly, dysregulated zinc homeostasis is linked to the pathogenesis of neurodegenerative diseases. Fluorescence sensors have emerged as powerful tools to unravel its role on the molecular level. With 20 members, the most prominent sensor family is the ZinPyr family that exploits a fluorescein platform equipped with usually two bis(2-pyridylmethyl)amine (DPA) as zinc binding units. Within this article, we report four new bright members of the ZinPyr family, ZP1(5-en), ZP1(6-en), ZP1(5-Me₂en), and ZP1(6-Me₂en), which are derived from the known sensors ZP1(5-CO₂H) and ZP1(6-CO₂H). Modification of these parent sensors with ethane-1,2-diamine (en) or <em>N</em>¹,<em>N</em>²-dimethylethane-1,2-diamine (Me₂en) yielded cell-permeable sensors that combine the low quantum yield of the zinc-free state Φ_free (0.165(0) – 0.190(9)) of the parent sensors with a high turn-on (5) and dynamic range (4.2 – 5.4). These properties make the new sensors among the brightest sensors in the ZinPyr family. Live cell imaging demonstrated their ability to detect intracellular zinc with an approximate turn-on of 2–3. The sensors showed a vesicular localization, with ZP1(6-en) and ZP1(5-Me₂en) also localizing in the nuclei.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"90 ","pages":"Article 127683"},"PeriodicalIF":3.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A selenomethionine deficient, high-fructose diet does not lead to cardiometabolic disorder in the selenocysteine lyase knockout mice","authors":"BK Shimada,&nbsp;NK Apo Takayama,&nbsp;KA Hallam,&nbsp;Santiago PJD,&nbsp;JY Yew,&nbsp;N Alfulaij,&nbsp;K Nakahara-Akita,&nbsp;AG Soares,&nbsp;MJ Berry,&nbsp;LA Seale","doi":"10.1016/j.jtemb.2025.127685","DOIUrl":"10.1016/j.jtemb.2025.127685","url":null,"abstract":"<div><h3>Background/purpose</h3><div>High-fructose consumption is a driver of cardiometabolic disorders and metabolic syndrome, and selenium (Se) deficiency further increases the risk of developing these diseases. Consuming high amounts of fructose induces insulin resistance and oxidative stress, and alters the cardiac lipidome. Se may reduce the detrimental impacts of fructose through its incorporation into selenoproteins like the glutathione peroxidases 1 and 4, (GPX1,4) and the thioredoxin reductase 1 (TXNRD1) whose primary function is to curb oxidative stress. When Se levels are limited, selenocysteine lyase (SCLY) decomposes selenocysteine (Sec) to hydrogen selenide (H₂Se), and loss of <em>Scly</em> results in metabolic syndrome in mice. However, it is unknown if SCLY is required to sustain the synthesis of critical antioxidant selenoproteins to prevent oxidative stress, cardiometabolic disorders, and metabolic syndrome caused by high-fructose consumption.</div></div><div><h3>Methods</h3><div>In this study, we analyzed cardiometabolic parameters, the cardiac lipidome, and the cardiac protein levels of GPX and TXNRD in male and female whole-body <em>Scly</em> knockout (<em>Scly</em> KO) mice fed a selenomethionine (SeMet) deficient, high-fructose diet.</div></div><div><h3>Results/conclusion</h3><div>We found that selenomethionine deficiency, coupled with high-fructose consumption does not lead to cardiometabolic disorder in the <em>Scly</em> KO mice, and suggests that there are compensatory mechanisms involving Se metabolism that are protective against fructose-induced cardiometabolic disorder.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"90 ","pages":"Article 127685"},"PeriodicalIF":3.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144329690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iron deficiency and iron deficiency anaemia in women of reproductive age: Sex- and gender-based risk factors and inequities","authors":"Jessie L. Burns ,&nbsp;Clara H. Miller ,&nbsp;Bénédicte Fontaine-Bisson ,&nbsp;Kristin L. Connor","doi":"10.1016/j.jtemb.2025.127684","DOIUrl":"10.1016/j.jtemb.2025.127684","url":null,"abstract":"<div><div>Iron deficiency (ID) is a serious public health problem that affects 20–25 % of the population and 52 % of pregnant people worldwide. Biologically female women (women) of reproductive age have a higher risk of developing ID due to the increased physiologic demand for iron required to support menstruation and pregnancy. If left untreated, ID can develop into iron deficiency anaemia (IDA), which affects one in three women between the ages of 15–49 years worldwide. Among women of reproductive age, those who are pregnant have the highest risk of developing ID and IDA due to increased iron requirements to support pregnancy and the developing fetus. Despite the high prevalence of ID and IDA, it remains underdiagnosed in reproductive-aged women and current treatment options are not well accepted. There is an urgent need to investigate novel strategies to ensure adequate iron status in women of reproductive age to prevent adverse health problems and promote healthy pregnancies. This review explored the critical role of iron in women's health by examining iron requirements throughout the lifespan, the physiology of iron absorption, factors affecting iron bioavailability, and the causes of ID and IDA. We discuss the limitations of current interventions for ID and IDA, and the need to develop effective and widely acceptable treatments for these conditions, particularly in women of reproductive age. The findings of this review suggest that current interventions for ID and IDA are inadequate and that sex biases exist in the diagnosis and management of ID and IDA in biologically female women.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"90 ","pages":"Article 127684"},"PeriodicalIF":3.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144491282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mediating role of whole epigenome DNA methylation signatures in the association between copper and SGA","authors":"Weiwei Wu ,&nbsp;Mengru Wang ,&nbsp;Shan Li ,&nbsp;Bole Zhang ,&nbsp;Weixuan Hu ,&nbsp;Yulin Li ,&nbsp;Yongliang Feng ,&nbsp;Yawei Zhang ,&nbsp;Suping Wang","doi":"10.1016/j.jtemb.2025.127688","DOIUrl":"10.1016/j.jtemb.2025.127688","url":null,"abstract":"<div><h3>Background</h3><div>Trace element Cu is associated with the risk of small for gestational age infants, but its underlying mechanism is still unclear. This study aims to explore the possible mechanism of Cu induced SGA from the perspective of DNA methylation.</div></div><div><h3>Methods</h3><div>We performed epigenome-wide DNA methylation analysis of umbilical cord blood from small for gestational age infants (SGA, n = 35) and appropriate for gestational age infants (AGA, n = 61) at birth to explore SGA related epigenome-wide DNA methylation sites. Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) was used to detect their umbilical cord blood Cu levels. Multiple linear regression models were used to explore the effect of Cu on SGA-related DNA methylation sites, and mediation analysis was further applied to explore the potential mediating factors of DNA methylation. The false discovery rate（FDR）was used for multiple comparisons.</div></div><div><h3>Results</h3><div>Compared with AGA (452 ng/mL), SGA infants (532 ng/mL) had higher umbilical cord blood Cu concentration (<em>P</em> &lt; 0.05). Seven Cu-related CpG sites were identified from epigenome-wide DNA methylation association analysis with SGA. Among them, a significant mediation effect of FLT3（cg10763141） methylation level in the association between high exposure level of Cu and SGA (<em>P</em> = 0.024). The mediation analysis showed a mediation proportion of 44.7 %.</div></div><div><h3>Conclusions</h3><div>Consistent with previous findings, we found that Cu was associated with the risk of SGA, and seven differentially methylated sites significantly associated with Cu were observed in the epigenome wide DNA methylation association study of SGA. FLT3（cg10763141）mediates the correlation between Cu and SGA. Cu may increase the risk of SGA by altering DNA methylation in certain genes (particularly FLT3). Our study provides new clues for the mechanism of SGA.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"90 ","pages":"Article 127688"},"PeriodicalIF":3.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144329689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heavy metal exposure and hepatitis C virus infection: A cross-sectional study","authors":"Humairat H. Rahman ,&nbsp;Weston R. Stokey ,&nbsp;Jonah Green ,&nbsp;Soyoung Jeon","doi":"10.1016/j.jtemb.2025.127687","DOIUrl":"10.1016/j.jtemb.2025.127687","url":null,"abstract":"<div><h3>Background</h3><div>Hepatitis C virus (HCV) is a Flaviviridae virus transmitted through contact with infected bodily fluids or utilization of non-sterile instruments. HCV has a high probability of progressing to a chronic infection, which leaves the individual susceptible to a multitude of other complications. Exposure to metals is common and difficult to avoid in the environment because of their widespread use in multiple industries.</div></div><div><h3>Objective</h3><div>In this study, barium (Ba), cadmium (Cd), cobalt (Co), manganese (Mn), molybdenum (Mo), lead (Pb), antimony (Sb), thallium (Tl), tin (Sn), and tungsten (W) were analyzed with their association on HCV infection prevalence.</div></div><div><h3>Methods</h3><div>The prevalence of HCV infection was analyzed using the Nutrition Examination Survey (NHANES) dataset for 2013–2020, including 2017-March 2020 pre-pandemic data. Weighted complex logistic regression analysis was used to examine associations of metal levels with HCV status for 18,073 participants aged 20–80 (n = 5751 urine samples collected out of 18,073).</div></div><div><h3>Results</h3><div>HCV was associated with increasing urinary Co [odds ratio (OR) 1.179; 95 % confidence interval (CI): 1.056, 1.316]. Tungsten (W) Q3 and Q4 showed a higher prevalence of HCV compared to W values below the detection limit (dl) with ORs 13.623 and 11.687, respectively. Molybdenum (Mo) showed significant increases in ORs of being HCV-positive across quartiles Q2, Q3, and Q4 (ORs 7.186, 5.472, and 8.579, respectively), compared to the lowest quartile (Q1) of Mo.</div></div><div><h3>Conclusion</h3><div>Overall, HCV infection was positively associated with exposure to Co, W, and Mo. These findings are significant in highlighting the intersection of environmental health and disease manifestation in public health research.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"90 ","pages":"Article 127687"},"PeriodicalIF":3.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Naringenin attenuates hepato-nephrotoxicity induced by aluminum nanoparticles through the mitigation of oxidative stress and apoptosis","authors":"Ravina Rai ,&nbsp;Virendra Singh ,&nbsp;Deepali Jat ,&nbsp;Siddhartha Kumar Mishra","doi":"10.1016/j.jtemb.2025.127686","DOIUrl":"10.1016/j.jtemb.2025.127686","url":null,"abstract":"<div><h3>Background</h3><div>Aluminum nanoparticles (Al-NPs) are produced by the biodegradation of naturally occurring aluminium metal which can induce physiological toxicities. Naringenin (NAR) is a natural flavonoid possessing therapeutic properties, particularly encountering the toxicity associated with heavy metals.</div></div><div><h3>Methods</h3><div>This study assessed the effects of NAR on Al-NPs-induced hepato-renal toxicity on mice exposed to Al-NPs (6 mg/kg b.w. per day) followed by administration of NAR (10 mg/kg b.w. per day).</div></div><div><h3>Results</h3><div>Al-NPs led to disturbances in the liver and kidney function enzymes and alterations in lipid profiles which were efficiently restored toward normal levels by treatment with NAR. NAR exhibited its protective action by regulating nitric oxide levels and reducing oxidative stress markers such as protein product oxidation, protein carbonylation, and lipid peroxidation. NAR restored the activity of antioxidant enzymes like superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase, as well as concentrations of both reduced and oxidized glutathione.</div></div><div><h3>Conclusions</h3><div>Histological analysis presents the protective effects of NAR against Al-NP-induced hepato-renal changes. Furthermore, NAR downregulated the expression of caspase-3 showing mitigation of apoptotic damage in the liver and kidneys of mice exposed to Al-NPs. This study highlights the antioxidant and anti-apoptotic properties of NAR, which counteracted hepato-renal toxicities.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"90 ","pages":"Article 127686"},"PeriodicalIF":3.6,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boosted wound healing and anticancer potential of polymer functionalized cerium oxide nanomaterials","authors":"Muhammad Khawar Abbas ,&nbsp;Yasir Javed ,&nbsp;Naveed Akhtar Shad ,&nbsp;Azhar Rasul ,&nbsp;Amna Bashir ,&nbsp;Syed Fazil Bin Farukh ,&nbsp;Heba Taha Abdulghani ,&nbsp;Wasim Abbas ,&nbsp;Aisha Sethi ,&nbsp;Muhammad Ramzan Khawar ,&nbsp;Dongwhi Choi","doi":"10.1016/j.jtemb.2025.127681","DOIUrl":"10.1016/j.jtemb.2025.127681","url":null,"abstract":"<div><h3>Background</h3><div>Wound recovery is an important part of treatment to prevent internal organelles from possible exposure to outer pathogens. In recent years, different therapies have been reported to enhance the natural wound healing process with faster and more effective tissue repair.</div></div><div><h3>Method</h3><div>The hydrothermal method has been endeavored to synthesize eco-friendly and cost-effective polymer (polyethylene glycol (PEG) and chitosan) functionalized cerium oxide nanoparticles (CeO₂ NPs). Wound healing potential was studied on Chorioallantoic Membrane Assay and <em>G. domesticus</em> wheras anticancer studies were conducted against HuH-7.0 (hepatic cancer cell line).</div></div><div><h3>Results</h3><div>Wound healing properties are studied initially through CAM assay which shows vein growth with chitosan-functionalized CeO₂ NPs. The topical wound healing studies on <em>G. domesticus</em> show speedy recovery and quicker completion of the skin remodeling phase, observed through the quality of the recovered skin. Additionally, PEGylation has promoted the anticancer potential of CeO₂ NPs against HuH-7.0 (hepatic cancer cell line) and the IC₅₀ value is 37.89 μg/mL.</div></div><div><h3>Conclusion</h3><div>The results show that PEG and chitosan coating have enhanced the therapeutic prospects of CeO₂ NPs.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"90 ","pages":"Article 127681"},"PeriodicalIF":3.6,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the chemotherapeutic potential of diosmetin ruthenium-p-cymene complex in bladder cancer treatment through the regulation of the PI3K/β-catenin/TJP1/AR signaling pathway","authors":"Sidhanta Sil,&nbsp;Sakuntala Gayen,&nbsp;Ishita Seal,&nbsp;Abhijit Das,&nbsp;Souvik Roy","doi":"10.1016/j.jtemb.2025.127680","DOIUrl":"10.1016/j.jtemb.2025.127680","url":null,"abstract":"<div><h3>Background</h3><div>Bladder cancer is the deadliest cause of cancer-related morbidity worldwide. The metal-flavonoid compound, diosmetin ruthenium-p-cymene complex, demonstrated a remarkable toxicological profile in different toxicological and genotoxicological studies. In this context, the study aimed to explore the chemotherapeutic effects of the diosmetin ruthenium-p-cymene complex in bladder cancer treatment through in vitro and in vivo approaches.</div></div><div><h3>Methods</h3><div>The in vitro assessment was carried out through cell viability assay, apoptotic assay by flow cytometry, and western blot study on both T24 and RT4 cells. Subsequently, the anticancer activity of the complex was assessed by BBN-induced bladder cancer in rats.</div></div><div><h3>Results</h3><div>As a result, the downregulation of TJP1, AR, and β-catenin expression with upregulation of PTEN expression was evaluated by western blot analysis. Cell cycle arrest was occurred and depicted via flow cytometric analysis. In the in-vivo study, the complex treatment significantly restored the normal bladder architecture. The induction of apoptotic events via downregulation of β-catenin, PI3K, Akt, and mTOR expression and upregulation of PTEN expression, showed in immunohistochemical analysis.</div></div><div><h3>Conclusion</h3><div>Consequently, the treatment with complex represented a promising chemotherapeutic activity by the modulation of tumor microenvironment through altering PI3K/ β-catenin/TJP1/AR facilitated signaling pathway involved with apoptosis induction in bladder carcinoma.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"90 ","pages":"Article 127680"},"PeriodicalIF":3.6,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}