Journal of Trace Elements in Medicine and Biology最新文献

{"title":"An integrated analytical approach for biomarker discovery in esophageal cancer: Combining trace element and oxidative stress profiling with machine learning","authors":"Omer Faruk Kocak ,&nbsp;Mehmet Emrah Yaman ,&nbsp;Atila Eroglu","doi":"10.1016/j.jtemb.2025.127678","DOIUrl":"10.1016/j.jtemb.2025.127678","url":null,"abstract":"<div><h3>Background</h3><div>Early detection of esophageal squamous cell carcinoma (ESCC) significantly improves survival rates, yet reliable biochemical biomarkers for early diagnosis remain limited. The aim of this study is to identify potential early diagnostic biomarkers by integrating trace element and oxidative stress profiling with machine learning. This study investigates alterations in trace elements and oxidative stress-related biomarkers in cancerous and adjacent healthy esophageal tissues (used as paired controls) using ICP-MS and spectrophotometric biochemical assays.</div></div><div><h3>Methods</h3><div>A total of 28 early-stage ESCC patients were included. Concentrations of 12 trace elements (Al, Cr, Mn, Fe, Co, Cu, Zn, Se, Sb, Hg and Pb) were measured via ICP-MS. Additionally, 11 oxidative stress and antioxidant markers were analyzed: SOD, CAT, GPx, PON, ARE, MPO, MDA, GSH, TAS, TOS and OSI.</div></div><div><h3>Results</h3><div>Statistical analysis revealed significant increases in Cu, Fe, and TOS levels and a marked decrease in Se in cancerous tissues. Strong correlations were observed among specific trace elements and antioxidant enzymes. Machine learning models, including XGBoost, Random Forest, LightGBM, SVM and Logistic Regression were employed to classify tissue types and identify key diagnostic markers. The XGBoost model achieved the highest performance (91.7 % accuracy, AUC = 0.97), and SHAP analysis highlighted Se and Zn as the most influential variables.</div></div><div><h3>Conclusion</h3><div>These findings demonstrate that the combined profiling of trace elements and oxidative stress biomarkers, enhanced with machine learning, can offer powerful tools for early ESCC diagnosis.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127678"},"PeriodicalIF":3.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144185081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of trace elements for the function and health of the skin","authors":"Geir Bjørklund ,&nbsp;Mariia Shanaida ,&nbsp;Tony Hangan ,&nbsp;Laura Kassym ,&nbsp;Assiya Kussainova ,&nbsp;Yuliya Semenova ,&nbsp;Emma Gheorghe ,&nbsp;Tetiana Gontova ,&nbsp;Volodymyr Voloshyn ,&nbsp;Roman Lysiuk ,&nbsp;Olena Voloshyn ,&nbsp;Volodymyr Shanaida ,&nbsp;Massimiliano Peana","doi":"10.1016/j.jtemb.2025.127674","DOIUrl":"10.1016/j.jtemb.2025.127674","url":null,"abstract":"<div><div>The skin, the body's largest organ, functions as both a protective shield and a complex sensory interface with the external environment. Its structural integrity and physiological functions are influenced by various intrinsic and extrinsic aging factors, often manifesting as wrinkles, fine lines, and pigmentation changes. Among the crucial determinants of skin health are trace elements, particularly zinc, copper, iron, selenium, manganese, and silicon, which are vital for maintaining skin homeostasis, supporting antioxidant defense, modulating inflammation, and facilitating tissue repair. Deficiencies or imbalances in these micronutrients have been implicated in a range of dermatological conditions, including acne, eczema, psoriasis, vitiligo, alopecia areata, and skin cancers. These elements can be replenished internally through diet and supplementation or externally via topical formulations. However, the widespread use of cosmetics and skincare products also introduces potential risks associated with toxic trace elements, underscoring the need for regulatory oversight and the development of safe, evidence-based skincare interventions. This narrative review explores the pivotal roles of beneficial trace elements in skin physiology and pathology, while addressing the implications of toxic metal exposure in dermatological health.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127674"},"PeriodicalIF":3.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144196133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Trace-elements driven up-regulation of secreted proteases expression in the human-pathogenic fungus Trichophyton rubrum” [J. Trace Elem. Med. Biol. 87 (2025) 127580]","authors":"Oliver Blechert ,&nbsp;Shanyu Lan ,&nbsp;Shuzhen Xiong ,&nbsp;Yuning Zou ,&nbsp;Peicong Li ,&nbsp;Jiewei Hu ,&nbsp;Jiayuan Li ,&nbsp;Ping Zhan","doi":"10.1016/j.jtemb.2025.127676","DOIUrl":"10.1016/j.jtemb.2025.127676","url":null,"abstract":"","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127676"},"PeriodicalIF":3.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144220798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ageing alters zinc, magnesium, and interleukin-8 levels in human dermal fibroblasts","authors":"Nishat Zakaria ,&nbsp;Matthew J. Smith ,&nbsp;David Messenger ,&nbsp;Ranjit Bhogal ,&nbsp;Richard Siow ,&nbsp;Wolfgang Maret","doi":"10.1016/j.jtemb.2025.127673","DOIUrl":"10.1016/j.jtemb.2025.127673","url":null,"abstract":"<div><h3>Background</h3><div>Ageing is linked to a decline in cellular functions, weakening the skin’s defence mechanisms. At advanced ages, deficiencies in micronutrients like zinc and magnesium are common. Zinc deficiency from inadequate intake or absorption can lead to skin issues such as delayed wound healing and eczema. Dysregulated zinc homeostasis can increase the risk of dermatitis and psoriasis.</div></div><div><h3>Objectives</h3><div>This study examined the relationship between zinc and magnesium levels and skin ageing and inflammation in human dermal fibroblasts from young and old donors.</div></div><div><h3>Methods</h3><div>Primary dermal fibroblasts from young (20–30 years) and older (62–75 years) donors were assessed. We evaluated oxidative stress, determined susceptibility to senescence, and analysed gene expression profiles while quantifying the secretion of the chemokine interleukin-8. Markers of zinc homeostasis were evaluated by assessing protein expression via western blotting, and both free zinc ions and total metal ion concentrations were quantified.</div></div><div><h3>Results</h3><div>Older fibroblasts showed increased oxidative stress and higher susceptibility to senescence. Interleukin-6 expression rose in aged cells, while interleukin-8 secretion declined significantly. Free zinc ion concentrations and zinc transporter 1 expression were elevated, along with total zinc and magnesium concentrations.</div></div><div><h3>Conclusions</h3><div>Reduced interleukin-8 secretion compromises immune regulation in ageing skin. Elevated free zinc ion levels and zinc transporter expression contribute to eventual cellular zinc loss, impairing inflammatory responses and weakening immunity. These findings highlight the critical role of zinc and magnesium micronutrient balance in maintaining skin health during ageing.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127673"},"PeriodicalIF":3.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144212726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotoxic and cytotoxic effect of vanadium inhalation and oral administration of Hintonia latiflora methanolic extract in mice","authors":"María Eugenia Cervantes-Valencia ,&nbsp;Marcela Rojas-Lemus ,&nbsp;Nelly López-Valdez ,&nbsp;Adriana González-Villalva ,&nbsp;Teresa I Fortoul","doi":"10.1016/j.jtemb.2025.127677","DOIUrl":"10.1016/j.jtemb.2025.127677","url":null,"abstract":"<div><h3>Introduction</h3><div>Environmental air pollution constitutes a significant problem for large cities, primarily due to anthropogenic activities such as burning fossil fuels, which have led to an escalation that release suspended particulate matter (PM);vanadium, a metal adhered to PM, leading to their entry into the bloodstream and subsequently V distribution to various organs. Research has demonstrated that exposure to vanadium (V) can induce single-strand breaks and the presence of micronuclei in cells. In addition to this, the use of herbal remedies has seen a marked increase on a global scale in recent years. It is estimated that more population makes use of these remedies without being aware of their potential health implications. Hintonia latiflora (Hl) is a herbal remedy commonly used, reported also as genotoxic.</div></div><div><h3>Objective</h3><div>To evaluate the genotoxic and cytotoxic effect of V inhalation and co-exposure with the administration of methanolic extract of Hl (HlMeOHe).</div></div><div><h3>Method</h3><div>Twenty-five male CD1 mice distributed in four groups were used: control, V, Tween 80 % (0.04 %), HlMeOHe (250 mg/kg per day) and V+HlMeOHe (250 mg/kg per day). Animals inhaled V twice a week. Blood samples were obtained at designated time points: 0, 24 and 72 hours; 7, 14 and 21 days. Subsequently, the samples were subjected to rigorous testing procedures, including the assessment of cell viability by fluorochromes, alkaline comet assay and micronucleus assay with acridine orange.</div></div><div><h3>Results</h3><div>The inhalation of V, either as a standalone treatment or in conjunction with <em>Hl</em>MeOHe, did not affect cell viability. Administration of <em>Hl</em>MeOHe, did not result in the generation of single-strand breaks, a finding that contrast with the effects of V. The co-exposure of V and <em>Hl</em>MeOHe exhibited from day 14–21 a significant decrease (p ≤ 0.05) in micronuclei assay, as well as a in single-strand breaks (p &lt; 0.05) compared against control group.</div></div><div><h3>Conclusion</h3><div>These results suggest that HlMeOHe is not cytotoxic or genotoxic maybe because of its phenolic compounds, reducing the genotoxic effect induced by V.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127677"},"PeriodicalIF":3.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing coral photosynthesis: The power of manganese-alginate gels","authors":"Gabriel Rodrigues Martins Moreira ,&nbsp;João Manoel De Lima Júnior ,&nbsp;Cassiana Seimi Nomura ,&nbsp;Jany Hellen Ferreira De Jesus ,&nbsp;Emmanuelle Uher ,&nbsp;Aurélie Dufour ,&nbsp;Maria-Isabelle Marcus Do Noscimiento ,&nbsp;Renaud Grover ,&nbsp;Christophe Migon ,&nbsp;Christine Ferrier-Pagès ,&nbsp;Breno Pannia Espósito","doi":"10.1016/j.jtemb.2025.127675","DOIUrl":"10.1016/j.jtemb.2025.127675","url":null,"abstract":"<div><h3>Introduction</h3><div>Scleractinian corals rely on symbiosis with Symbiodiniaceae, a family of marine dinoflagellates, for photosynthetic products, which sustains their growth in nutrient-poor environments. Manganese (Mn) is a critical element for photosynthesis as an essential cofactor for the oxygen-evolving complex of the photosynthetic machinery. Also, Mn is an essential metal involved in antioxidant mechanisms that maintain normal cellular function in heat stressed corals. <em>Objectives</em>: This study aims to investigate the effects of different Mn species on photosynthetic efficiency in the coral <em>Stylophora pistillata</em> under thermal stress. A Mn-alginate device for controlled Mn supplementation was developed for this purpose. <em>Methods</em>: Coral nubbins were exposed to the following Mn species: manganese(II) chloride (MnCl₂), manganese(II) ethylenediamine-N,N′-disuccinate (MnEDDS), manganese(II) desferrioxamine (MnDFO), and manganese(III) citrate (MnCIT). Photosynthetic parameters were measured using fluorometry. Mn-alginate gel dishes were prepared for controlled release, and their impacts on coral health indicators were evaluated at 26 °C and 31 °C, including photosynthetic efficiency, oxygen production, and symbiont density. <em>Results</em>: Free Mn(II) (MnCl₂) and MnEDDS significantly enhanced photosynthetic efficiency. Mn-alginate effectively delivered Mn in controlled bursts. Under thermal stress, Mn supplementation improved photosynthetic activity and favored symbiont density. Mn-alginate dishes were stable in seawater and biocompatible, releasing Mn optimally at elevated temperatures. <em>Conclusion</em><strong>:</strong> Mn-alginate gel dishes are an efficient and safe method for improving coral photosynthesis and mitigating thermal stress impacts, supporting reef conservation efforts in a changing climate.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127675"},"PeriodicalIF":3.6,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overview of the effects of heavy metals on the reproductive health of males in the genus Mytilus spp.","authors":"Carmela Marinaro ,&nbsp;Rosaria Notariale ,&nbsp;Mirko Cardillo ,&nbsp;Maria Grazia Guarnieri ,&nbsp;Marco Trifuoggi ,&nbsp;Filomena Mottola ,&nbsp;Lucia Rocco ,&nbsp;Gennaro Lettieri ,&nbsp;Luigi Montano ,&nbsp;Marina Piscopo","doi":"10.1016/j.jtemb.2025.127672","DOIUrl":"10.1016/j.jtemb.2025.127672","url":null,"abstract":"<div><div>This review examines the reprotoxic effects of key heavy metals, specifically nickel, chromium, zinc, copper, mercury and cadmium, on the reproductive health of bivalve molluscs of the genus <em>Mytilus spp</em>. which serve as important bioindicators in marine ecosystems. Exposure to even sub-lethal concentrations of these metals induces oxidative stress, DNA damage and changes in chromatin structure, which are thought to affect fertility. These metals, known for their persistence and bioaccumulation, interfere with critical reproductive processes by also altering the functions of protamine-like proteins (PLs), the main nuclear basic proteins of sperm chromatin of these organisms. Moreover, Seasonal variations influence the toxicity of these metals, coinciding with critical phases of gametogenesis and amplifying their detrimental effects during reproductive cycles. The study highlights the urgent need for comprehensive biomonitoring programmes, as the sensitivity of <em>Mytilus spp</em>. to environmental contaminants provides valuable insights into the health of marine ecosystems. Given their economic importance and ecological role as filter feeders, the impact of heavy metal pollution on these bivalve molluscs goes beyond increased environmental pollution and threatens biodiversity and seafood safety. To mitigate these risks, strict pollution controls and further research into molecular toxicology are essential. Understanding this will help to develop effective strategies to conserve marine life and maintain the ecosystem benefits provided by these sentinel species.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127672"},"PeriodicalIF":3.6,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144139351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The safety assessment of cinnabar: Effects of co-administration with selenium on renal toxicity in mice","authors":"Ziyan Han ,&nbsp;Yuzhou Zhuo ,&nbsp;Zhihui Dai ,&nbsp;Xinhui Zhang ,&nbsp;Bingqian Zhu ,&nbsp;Yuyan Liu ,&nbsp;Zhuoyu Chai ,&nbsp;Jiayi Wu ,&nbsp;Lijun Chen","doi":"10.1016/j.jtemb.2025.127671","DOIUrl":"10.1016/j.jtemb.2025.127671","url":null,"abstract":"<div><h3>Background</h3><div>Cinnabar is a mercury-containing mineral traditionally used in Chinese medicine and can induce kidney injury via mercury toxicity. Given that cinnabar contains elements such as selenium, it is reasonable to hypothesize that its multi-element composition may regulate nephrotoxicity through intermetallic interactions. To validate this hypothesis and clarify the distinct renal toxicity between cinnabar and single mercury compounds, we compared the nephrotoxic effects of cinnabar, Zhu-Sha-An-Shen-Wan (ZSASW), mercuric sulfide (HgS), and mercuric nitrate (Hg(NO₃)₂). By co-administration with sodium selenite (Na₂SeO₃), this study related to the nephrotoxicity of cinnabar was improved from the perspective of metal-element interactions, which provided a new perspective for the safety assessment of mercury-containing medicines.</div></div><div><h3>Methods</h3><div>Mice were gavaged with 0.5 % CMC-Na solution, cinnabar (50.0 and 200 mg/kg), HgS (50.0 mg/kg), Na₂SeO₃ (1.00 mg/kg), cinnabar (50.0 mg/kg)+Na₂SeO₃ (1.00 mg/kg), HgS (50.0 mg/kg)+Na₂SeO₃ (1.00 mg/kg), ZSASW (600 mg/kg), ZSASW (600 mg/kg)+Na₂SeO₃ (1.00 mg/kg) or Hg(NO₃)₂ (0.900 mg/kg in Hg²⁺) daily for 30 days. Renal histopathology was assessed by H&amp;E staining. Related protein expression was measured by Western blotting. Renal total Hg (THg) concentration in kidney was determined by cold vapor atomic fluorescence spectroscopy.</div></div><div><h3>Result</h3><div>Western blotting revealed significantly decreased OAT1 and GPX4 levels in all experimental groups compared to the Control group (<em>P</em> &lt; 0.05). NF-κB activation occurred in the Hg(NO₃)₂, Cinnabar-H, HgS, and HgS-Na₂SeO₃ groups. Hg(NO₃)₂ administration caused a significant decline in body weight growth rate (<em>P</em> &lt; 0.001), severe renal tubular degeneration with epithelial cell swelling, and the highest renal THg concentration (773.77 % exceeding the Control group). Na₂SeO₃ alone induced inflammatory infiltration and tubular epithelial degeneration. The Cinnabar-H and HgS groups exhibited distinct renal damage (localized tubular degeneration and vascular hyaline degeneration, respectively) with elevated renal THg concentration (330.74 % and 347.95 % exceeding the Control group). The Cinnabar-L, Cinnabar-Na₂SeO₃, ZSASW, and ZSASW-Na₂SeO₃ groups maintained normal histology despite increased renal mercury content (227.46 %, 40.57 %, 67.2 %, and 556.56 % exceeding the Control group). Co-administration of Na₂SeO₃ with HgS restored OAT1 and GPX4 expression (<em>P</em> &lt; 0.001), suppressed NF-κB activation, and minimally altered renal mercury accumulation (RSD=0.45 %). Na₂SeO₃ reduced mercury levels in the cinnabar-treated mice (57.07 % reduction) but increased accumulation in ZSASW-treated mice (292.65 % increase).</div></div><div><h3>Conclusion</h3><div>This study confirms th","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127671"},"PeriodicalIF":3.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144090432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of M86 and M87 compounds against lead, cadmium, and arsenic toxicity in 2D and 3D liver cell models","authors":"Hamdi Temel ,&nbsp;Serap Yalçın Azarkan ,&nbsp;Serkan Şahin","doi":"10.1016/j.jtemb.2025.127665","DOIUrl":"10.1016/j.jtemb.2025.127665","url":null,"abstract":"<div><div>Increasing environmental pollution increases the risk of human exposure to toxic metals. Therefore, there is a need for substances to protect individuals against the harmful effects caused by toxic metals. Bu çalışma, 3-metoksi katekol bileşiğinin 1,4-fenil diboronik asitle 1:1 ve 1:2 mol ratios of 3-methoxy catechol compound with 1,4-phenyl diboronic acid (1:1 and 1:1) and 1,4-bis(4-methoxybenzo[<em>d</em>][1,3,2]dioxaborol-2-yl)phenyl)boronic acid (M86) and 1,4-bis(4-methoxybenzo[<em>d</em>][1,3,2]dioxaborol-2-yl)benzene (M87) against lead (Pb), cadmium (Cd) and arsenic (As) toxicity in THLE-2 liver cell line. The structures of synthesized compounds M86 and M87 were characterized by 1 H, 13 C NMR, LC-MS-IT-TOF, UV-Vis., FTIR. The biological activities of these compounds were evaluated by DPPH, ABTS, CUPRAC, anticholinesterase, antiurease and antithyrosinase tests. 2D and 3D cell models were used in THLE-2 cell line. The protective effects of M86 and M87 against Pb, Cd and As toxicity were examined by XTT test and ATP colorimetric method and IC50 values were determined. In antioxidant tests, it was observed that M86 and M87 exhibited high activity in ABTS, DPPH and CUPRAC tests compared to standard antioxidants ?-tocopherol (?-TOC) and butylated hydroxytoluene (BHT). Enzyme inhibition tests showed that M86 and M87 significantly suppressed acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzyme activities. These compounds were found to reverse the decrease in cell proliferation following Pb, Cd and As exposure. In conclusion, M86 and M87 have the potential to be versatile therapeutic agents that provide effective protection against metal toxicity. In the future, with the evaluation of the efficacy of these compounds in in vivo models and clinical studies, it is thought that their use against metal toxicity may be possible.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127665"},"PeriodicalIF":3.6,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of multi-metal exposure with hyperuricemia in middle-aged and older adults: A cross-sectional study in rural Guangxi, China","authors":"Qinyi Guan ,&nbsp;Kaisheng Teng ,&nbsp;Qiumei Liu ,&nbsp;Songju Wu ,&nbsp;Lei Luo ,&nbsp;Linhai Zhao ,&nbsp;Jiahui Rong ,&nbsp;Wenjia Jin ,&nbsp;Tiantian Zhang ,&nbsp;Mo Xiaoting ,&nbsp;Cai Jiansheng ,&nbsp;Zhiyong Zhang ,&nbsp;Jian Qin","doi":"10.1016/j.jtemb.2025.127670","DOIUrl":"10.1016/j.jtemb.2025.127670","url":null,"abstract":"<div><h3>Background</h3><div>Hyperuricemia constitutes a significant global public health concern, particularly among the elderly, and is associated with environmental factors such as metal exposure. In China, the predominant focus of existing research has been on urban populations, which leaves the relationship between hyperuricemia and ecological exposures in rural populations inadequately explored. This study investigates the association between hyperuricemia and exposure to multiple metals among elderly residents in rural China.</div></div><div><h3>Methods</h3><div>Cross-sectional survey data were collected from middle-aged and elderly individuals residing in Yao Autonomous County of Gongcheng, Guangxi, China, from 2018 to 2019. The analytical techniques employed included least absolute shrinkage and selection operator (LASSO) regression, binary logistic regression and Bayesian kernel machine regression (BKMR) to examine the association between exposure to multi-metal mixtures and hyperuricemia.</div></div><div><h3>Results</h3><div>Logistic regression results indicated a negative association between magnesium (OR=0.634, 95 % CI=0.476‐0.867, Q3) and hyperuricemia, while iron, nickel, strontium, molybdenum, and tin were positively associated with hyperuricemia (all P &lt; 0.05). The Bayesian kernel machine regression analysis revealed a significant overall positive correlation between hyperuricemia and plasma polymetallic mixtures, with notable contributions from Sr (PIP=0.991) and Fe (PIP=0.749), particularly when all metals were at their respective 50th percentiles. Exposure-response relationships were consistent with the logistic regression results. Plasma Sr was positively correlated with hyperuricemia at different levels of other metals (p25, p50, p75).</div></div><div><h3>Conclusion</h3><div>This study suggests that exposure to multi-metal mixtures is positively associated with hyperuricemia, with Sr and Fe being the most significant contributors.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127670"},"PeriodicalIF":3.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}