Journal of Trace Elements in Medicine and Biology最新文献

{"title":"High levels of the health-relevant antioxidant selenoneine identified in the edible mushroom Boletus edulis","authors":"","doi":"10.1016/j.jtemb.2024.127536","DOIUrl":"10.1016/j.jtemb.2024.127536","url":null,"abstract":"<div><h3>Background</h3><div>Selenoneine, the selenium analogue of the sulfur antioxidant ergothioneine, has been ascribed a multitude of beneficial health effects. Natural nutritional sources for this selenium species are, hence, of high interest. So far marine fish is the only significant selenoneine source consumed by larger parts of the population worldwide.</div></div><div><h3>Methods</h3><div>As selenoneine and ergothioneine share their biosynthetic pathways and transport mechanisms and the popular edible porcini mushroom <em>Boletus edulis</em> is rich in ergothioneine and selenium, we conducted a preliminary study investigating a composite sample of two specimens of <em>B. edulis</em> for their selenoneine content by HPLC coupled to elemental and molecular mass spectrometry after aqueous extraction.</div></div><div><h3>Results</h3><div>Selenium speciation analysis by HPLC-ICPMS revealed that ca. 860 µg Se kg⁻¹ wet mass (81 % of the total Se) co-eluted with a selenoneine standard and a minor selenium species with Se-methylselenoneine. The presence of selenoneine was rigorously proven by HPLC-ESI-Orbitrap MS. The selenoneine content of the investigated specimens of <em>B. edulis</em> was higher than that of commonly consumed muscle of marine fish species, like tuna or mackerel.</div></div><div><h3>Conclusion</h3><div>This is the first report of a terrestrial food source containing significant selenoneine levels. Our results suggest that <em>B. edulis</em> might represent a complementary natural supply with this health-relevant selenium species for humans.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0946672X24001561/pdfft?md5=ab6406e2c5511e6c76c56fed2f8b5c43&pid=1-s2.0-S0946672X24001561-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142314949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intake and excretion of cadmium of Japanese adult","authors":"","doi":"10.1016/j.jtemb.2024.127535","DOIUrl":"10.1016/j.jtemb.2024.127535","url":null,"abstract":"<div><h3>Objective</h3><div>It is the aim of this study if the recent Cd intake and excretion is consistent with the regression equation proposed by Ikeda et al. (<em>Environ. Health Prev. Med.</em> 20 (2015) 455–459) which utilized intake/excretion data of 3 decades ago</div></div><div><h3>Method</h3><div>Matched duplicate diet and spot urine samples were collected in 2017–2019 from 150 Japanese adults (85 females and 65 males, mean age being 44.9) for the estimation of daily Cd intake and urinary excretion. Cd concentration in diet and urine was measured by ICP mass spectrometry.</div></div><div><h3>Result</h3><div>Geometric mean (geometric standard deviation) of Cd intake was 12.1 (1.67) μg/person/day or 0.206 (1.66) μg/kg body wt./day. Urinary Cd concentration was 0.54 (2.6) μg/g creatinine or 0.67 μg/L (2.3) (gravimetric correction). These were lower than those reported previously for Japanese populations. Urinary concentration was significantly higher in females than in males for both creatinine and gravimetric corrections. Dietary intake was higher in males than in females but this difference diminished after body weight correction. There was no significant correlation between Cd intake and urinary concentration on individual basis, which was expected because urinary Cd concentration reflects Cd body burden but not recent intake level. Meanwhile, the geometric mean intake and urine concentration was consistent with the regression equation proposed by Ikeda et al., which was based on intake and excretion surveys for 30 non-exposed Japanese female populations.</div></div><div><h3>Conclusion</h3><div>This result supported that the regression equation of Ikeda et al. could be used for conversion of urinary Cd excretion level of a population to intake level.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel copper complex inhibits the proteasome in skin squamous cell carcinoma induced by DMBA in mice","authors":"","doi":"10.1016/j.jtemb.2024.127533","DOIUrl":"10.1016/j.jtemb.2024.127533","url":null,"abstract":"<div><div>The proteasomal system is becoming a target for the treatment of several diseases, especially in cancer therapy. The present study aims to develop a novel copper complex that inhibits the proteasome in skin squamous cell carcinoma. New molecules based on the copper complex were synthesized for the first time to assess their potential as proteasome inhibitors, specifically targeting squamous cell carcinoma induced by 7,12-dimethylbenz(a)anthracene (DMBA) in mouse models. Fourier Transform Infrared (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), nuclear magnetic resonance (NMR), and energy dispersive X-ray analysis (EDX) were carried out to characterize this new copper complex. Notably, the presence of a papilloma (skin tumor) was confirmed by histopathological analysis. Subsequent investigation included the quantification of proteasome levels using a sandwich ELISA test, and the catalytic activity of the 20S proteasome was determined by measuring the fluorescence emitted after the cleavage of 7-amino-4-methylcoumarin (AMC). Hence, X-ray crystallography indicates that all Cu atoms are five-coordinated in a square-pyramidal configuration and biological activity of copper Schiff base complex, which exhibits high proteasome inhibitory activities with particular selectivity of <em>β</em>5 subunit. The pharmacokinetic properties (ADMET) of the copper complex named Cu(L1) showed encouraging results with very low toxicity, distribution, and absorption. Structure–activity relationship (SAR) information obtained from Cu(L1) demonstrated its selectivity and potent inhibition for <em>β</em>5 subunit. In this regard, this copper complex has emerged as a novel therapy for skin cancer.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142314948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The concentrations of microelements in forest mushrooms are influenced by soil pH and C/N ratio and less by stand characteristics","authors":"","doi":"10.1016/j.jtemb.2024.127534","DOIUrl":"10.1016/j.jtemb.2024.127534","url":null,"abstract":"<div><p>The fruitbodies or sporocarps formed by mushrooms can accumulate mineral elements, such as selenium, zinc or copper, making them an important source of micronutrients essential to humans. However, the literature about environmental factors affecting mineral composition in mushrooms is scarce and limited to the ambiguous impact of soil properties and region. In our study, we investigated the effects of tree stand characteristics (tree species and tree canopy cover), understory cover, and soil properties (pH and C/N ratio of the soil) on the concentration of minerals in six edible mushroom species: <em>Laccaria laccata</em>, <em>L. proxima</em>, <em>L. amethystina</em>, <em>Lepista nuda</em>, <em>Lycoperdon perlatum</em>, and <em>Calvatia excipuliformis</em>, collected on 20 plots covered by stands of different tree species composition and varying in the understory cover. We estimated the concentration of eight elements (Zn, Se, Mg, Mn, Cu, Co, Cr, Mo) using the ICP-MS (Inductively Coupled Plasma - Mass Spectrometry) technique and compared their concentration between the plots, using ordination and linear regression methods. Our research revealed that mushroom species identity, including its ecological role and phylogenetic affinity, had the greatest effect on the mineral composition of mushrooms. The effect of environmental factors depended also on the micronutrient identity. Some elements were not affected at all (Co, Cr), some only by soil reaction or C/N ratio (Mn, Mg, Cu), while others were influenced by both tree stand characteristics and soil properties (Se, Zn, Mo). This knowledge enables us to maximize the content of minerals in harvested mushrooms by collecting them in specific areas. For example, mushrooms, which are sources of Se and Zn, can be gathered in coniferous forests characterized by acid soils, low canopy cover, and minimal understory cover. This targeted collection approach can effectively increase the mineral content in harvested mushrooms, thereby enhancing their health benefits.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0946672X24001548/pdfft?md5=2237eea8e979b4ad16e8a956cab4ba76&pid=1-s2.0-S0946672X24001548-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142272805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in breast cancer therapy: The promise of copper nanoparticles","authors":"","doi":"10.1016/j.jtemb.2024.127526","DOIUrl":"10.1016/j.jtemb.2024.127526","url":null,"abstract":"<div><h3>Background</h3><p>Breast cancer (BC) is the most prevalent cancer among women worldwide and poses significant treatment challenges. Traditional therapies often lead to adverse side effects and resistance, necessitating innovative approaches for effective management.</p></div><div><h3>Objective</h3><p>This review aims to explore the potential of copper nanoparticles (CuNPs) in enhancing breast cancer therapy through targeted drug delivery, improved imaging, and their antiangiogenic properties.</p></div><div><h3>Methods</h3><p>The review synthesizes existing literature on the efficacy of CuNPs in breast cancer treatment, addressing common challenges in nanotechnology, such as nanoparticle toxicity, scalability, and regulatory hurdles. It proposes a novel hybrid method that combines CuNPs with existing therapeutic modalities to optimize treatment outcomes.</p></div><div><h3>Results</h3><p>CuNPs demonstrate the ability to selectively target cancer cells while sparing healthy tissues, leading to improved therapeutic efficacy. Their unique physicochemical properties facilitate efficient biodistribution and enhanced imaging capabilities. Additionally, CuNPs exhibit antiangiogenic activity, which can inhibit tumor growth by preventing the formation of new blood vessels.</p></div><div><h3>Conclusion</h3><p>The findings suggest that CuNPs represent a promising avenue for advancing breast cancer treatment. By addressing the limitations of current therapies and proposing innovative solutions, this review contributes valuable insights into the future of nanotechnology in oncology.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142243981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of heavy metal levels in cow's milk and associated health risks in the vicinity of the MIDROC Laga Dambi gold mine in Ethiopia","authors":"","doi":"10.1016/j.jtemb.2024.127529","DOIUrl":"10.1016/j.jtemb.2024.127529","url":null,"abstract":"<div><h3>Introduction</h3><p>The possible health effects of consuming milk contaminated with heavy metals have been the subject of considerable concern worldwide.</p></div><div><h3>Objective</h3><p>The aim of this study was to determine the level of heavy metals in cow's milk in the vicinity of MIDROC Laga Dambi gold mine and to assess their possible health risks for consumers.</p></div><div><h3>Methods</h3><p>Nine composite samples were formed by aggregating 243 milk samples obtained in triplicates from 81 domestic milk-producing households. Inductively coupled plasma-optical emission spectroscopy was used to measure the amount of heavy metals after samples digestion under optimal conditions.</p></div><div><h3>Results</h3><p>The heavy metals concentrations obtained were 13.913˗7.843, 9.505˗3.589, 5.972˗3.147, 2.288˗1.851, 0.403˗0.143, 0.436˗0.128, 0.26˗0.153, 0.143˗0.048, 0.160˗ND (not detected), and 0.140˗ND mgkg⁻¹for Fe, Zn, Pb, Mn, Hg, Cr, Cd, As, Ni, and Co, respectively. Of the heavy metals identified, the levels of Pb, As, Cd, and Hg exceeded the recommended value. Based on the estimated daily intake (EDI), the total health quotient (THQ) is higher than unity even for Pb alone. It has been found that the consumption of cow milk increases the health index (HI) by 2.972. Ninety five percent of the HI in the study area was explained by the toxic heavy metals (Pb, Cd, As, and Hg) in the cow milk, which were found to be beyond the safe limit.</p></div><div><h3>Conclusion</h3><p>This demonstrates that there is a health risk to the population who consume cow’s milk sourced from the vicinity of MIDROC Laga Dambi gold mine. To safeguard the public’s health, we advised strict monitoring and legislative control for the safety of cow’s milk originating from study area.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142243980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Copper excess in psychiatric disorders: a focus on mood spectrum disorders and sex","authors":"","doi":"10.1016/j.jtemb.2024.127532","DOIUrl":"10.1016/j.jtemb.2024.127532","url":null,"abstract":"<div><h3>Background</h3><p>Meta-analyses show increased copper (Cu) levels in major depression disorder. However, the association of Cu biomarkers with clinical classification in other mental health disorders has not been fully explored.</p></div><div><h3>Methods</h3><p>To this aim, we compared an extensive panel of Cu biomarkers, composed of Cu, ceruloplasmin (Cp) Cp activity, Cp specific activity, Cu not bound to ceruloplasmin (non-Cp Cu, also known as ‘free’ copper) in 171 consecutive patients affected by psychiatric disorders and in 61 healthy controls (HC) using MANOVA adjusting for the effect of sex and age, and studied their association with the clinical scale outcomes at psychiatric examination, namely Global Assessment of Functioning, Clinical Global Impression, and Brief Psychiatric Rating Scale.</p></div><div><h3>Results</h3><p>individuals with psychiatric disorders were classified as 109 patients affected by mood spectrum disorders (MSD), 20 patients with schizophrenia spectrum disorders (SSD), and 42 with personality disorders (PD). Cu and non-Cp Cu were increased in psychiatric individuals than in HC, which also differed among the patients stratified per the clinical classification, being higher in the MSD individuals. The analysis stratified for sex revealed that women from the patient group, and specifically from the MSD group, had increased levels of Cu and non-Cp Cu than healthy women, while no difference was revealed in men. A logistic regression model considering the effect of sex and age revealed that non-Cp Cu could explain 26 % increased odds of having MSD per µmol/L unit increase (OR = 1.26; p = 0.0008; 95 % CI 1.099–1.436), that reached 40 % when considering only women. This result was driven by non-Cp Cu that correctly classified 64.1 % MSD (70 % in women) individuals vs. HC in a decision tree model, with values higher than 2.1 µmol/L which could distinguish the majority of MSD patients (86.3 % MSD vs. 13.7 % HC in women). None of the biological variables under study correlated with outcomes of the clinical scales, substances, or alcohol abuse.</p></div><div><h3>Conclusion</h3><p>Current results suggest mild Cu toxicity in women with MSD, as revealed by a value of non-Cp Cu higher than 2.1 µmol/L, which can be further investigated to assess its potential diagnostic accuracy in bigger and longitudinal cohorts.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0946672X24001524/pdfft?md5=e008593a0ba1378aa5ff35527a511a9c&pid=1-s2.0-S0946672X24001524-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142243977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The correlation between single and mixed trace elements exposure in systemic lupus erythematosus: A case-control study","authors":"","doi":"10.1016/j.jtemb.2024.127524","DOIUrl":"10.1016/j.jtemb.2024.127524","url":null,"abstract":"<div><h3>Background</h3><p>Recent studies have shown an association between trace elements and systemic lupus erythematosus (SLE), but the relationship between trace elements and SLE is still unclear. This study aims to determine the distribution of plasma trace elements in newly diagnosed SLE patients and the association between these essential and toxic element mixtures and SLE.</p></div><div><h3>Methods</h3><p>In total, 110 SLE patients and 110 healthy controls were included. Blood samples were collected. 15 plasma trace elements were quantified using an inductively coupled plasma mass spectrometer (ICP-MS). Multivariate logistic regression, restricted cubic spline (RCS), weighted quantile sum (WQS) regression, quantile g-computation (qgcomp), and Bayesian kernel machine regression (BKMR) are used to analyze the association between single and mixed exposure of elements and SLE.</p></div><div><h3>Results</h3><p>The logistic regression model shows that, plasma lithium (Li) [OR (95 % CI): 1.963 (1.49–2.586)], vanadium (V) [OR (95 % CI): 2.617(1.645–4.166)] and lead (Pb) [OR (95 % CI): 1.603(1.197–2.145)] were positively correlated with SLE, while selenium (Se) [OR (95 % CI): 0.055(0.019–0.157)] and barium (Ba) [OR (95 % CI): 0.792(0.656–0.957)] had been identified as protective factors for SLE. RCS results showed a non-linear correlation between the elements Li, V, Ni, copper, Se, rubidium and SLE. In addition, WQS regression, qgcomp, and BKMR models consistently revealed significant positive effects of plasma Li and Pb on SLE, as well as significant negative effects of plasma Se.</p></div><div><h3>Conclusions</h3><p>Exposure to heavy metals such as Li and Pb is significantly positively correlated with SLE, but Se may be protective factors for SLE. In addition, there is a nonlinear correlation between the elements Li and Se and SLE, and there are complex interactions between the elements. In the future, larger populations and prospective studies are needed to confirm these associations.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142243979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The smallest traces of crime: Trace elements in forensic science","authors":"","doi":"10.1016/j.jtemb.2024.127527","DOIUrl":"10.1016/j.jtemb.2024.127527","url":null,"abstract":"<div><h3>Background</h3><p>Securing the evidence in various investigative situations is often associated with trace analysis, including fingerprints or blood groups. However, when classic and conventional methods fail, trace elements, such as copper, zinc, fluorine, and many others found in exceedingly insignificant amounts in organisms, may prove useful and effective.</p></div><div><h3>Methods</h3><p>The presented work reviews articles published between 2003 and 2023, describing the use of trace elements and the analytical methods employed for their analysis in forensic medicine and related sciences.</p></div><div><h3>Results &amp; conclusion</h3><p>Trace elements can be valuable as traces collected at crime scenes and during corpse examination, aiding in determining characteristics like the sex or age of the deceased. Additionally, trace elements levels in the body can serve as alcohol or drug poisoning markers. In traumatology, trace elements enable the identification of various instruments and the injuries caused by their use.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0946672X24001470/pdfft?md5=29092a086ff3882d8a4812b379cf1a21&pid=1-s2.0-S0946672X24001470-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142243978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring serum trace element shifts: Implications for cervical intraepithelial neoplasia","authors":"","doi":"10.1016/j.jtemb.2024.127531","DOIUrl":"10.1016/j.jtemb.2024.127531","url":null,"abstract":"<div><h3>Background</h3><p>Cervical intraepithelial neoplasia (CIN) represents a premalignant state presumably related to perturbations in circulating levels of trace elements.</p></div><div><h3>Materials and methods</h3><p>Employing inductively coupled plasma mass spectrometry (ICP-MS), we quantified essential and toxic trace elements in the sera of 60 women diagnosed with CIN and 60 age-matched healthy counterparts.</p></div><div><h3>Results</h3><p>Our investigation revealed a noteworthy higher levels in serum of Mn, Zn, and Pb, as well as lower levels in Ni, Se, Rb, and Mo levels within the CIN cohort. Levels of Mn, Zn, and Pb were higher by approximately 5.5-fold, 3.0-fold, and 7.5-fold, respectively, while Mo levels exhibited an approximate 4.5-fold reduction in CIN sera compared to the control group. While the study provided valuable insights into trace element variations, it’s important to note that the adult Serbian population is considered Zn-deficient, so the Zn data should be interpreted with caution. Age stratification (30–40 vs. 40–50 vs. 50–60 years), smoking status (smokers vs. nonsmokers), and CIN severity (CIN 2 vs. CIN 3) yielded no significant disparities in elemental profiles. Among the 10 proposed ratios, 5 demonstrated a significant surge in CIN sera relative to controls: Mn/Se, Mn/Mo, Zn/Se, Zn/Mo, and Se/Mo. Correlation analysis of trace element levels revealed a predominantly consistent pattern between CIN cases and healthy subjects, except for Zn and its negative correlations (antagonistic interactions) with other analyzed trace elements.</p></div><div><h3>Conclusion</h3><p>Our findings underscore differences in serum levels of specific trace elements in CIN cases versus controls, implicating their potential involvement in the underlying pathophysiological cascades culminating in cervical neoplasms.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142172155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}