Journal of Trace Elements in Medicine and Biology最新文献

{"title":"Genotoxic and cytotoxic effect of vanadium inhalation and oral administration of Hintonia latiflora methanolic extract in mice","authors":"María Eugenia Cervantes-Valencia ,&nbsp;Marcela Rojas-Lemus ,&nbsp;Nelly López-Valdez ,&nbsp;Adriana González-Villalva ,&nbsp;Teresa I Fortoul","doi":"10.1016/j.jtemb.2025.127677","DOIUrl":"10.1016/j.jtemb.2025.127677","url":null,"abstract":"<div><h3>Introduction</h3><div>Environmental air pollution constitutes a significant problem for large cities, primarily due to anthropogenic activities such as burning fossil fuels, which have led to an escalation that release suspended particulate matter (PM);vanadium, a metal adhered to PM, leading to their entry into the bloodstream and subsequently V distribution to various organs. Research has demonstrated that exposure to vanadium (V) can induce single-strand breaks and the presence of micronuclei in cells. In addition to this, the use of herbal remedies has seen a marked increase on a global scale in recent years. It is estimated that more population makes use of these remedies without being aware of their potential health implications. Hintonia latiflora (Hl) is a herbal remedy commonly used, reported also as genotoxic.</div></div><div><h3>Objective</h3><div>To evaluate the genotoxic and cytotoxic effect of V inhalation and co-exposure with the administration of methanolic extract of Hl (HlMeOHe).</div></div><div><h3>Method</h3><div>Twenty-five male CD1 mice distributed in four groups were used: control, V, Tween 80 % (0.04 %), HlMeOHe (250 mg/kg per day) and V+HlMeOHe (250 mg/kg per day). Animals inhaled V twice a week. Blood samples were obtained at designated time points: 0, 24 and 72 hours; 7, 14 and 21 days. Subsequently, the samples were subjected to rigorous testing procedures, including the assessment of cell viability by fluorochromes, alkaline comet assay and micronucleus assay with acridine orange.</div></div><div><h3>Results</h3><div>The inhalation of V, either as a standalone treatment or in conjunction with <em>Hl</em>MeOHe, did not affect cell viability. Administration of <em>Hl</em>MeOHe, did not result in the generation of single-strand breaks, a finding that contrast with the effects of V. The co-exposure of V and <em>Hl</em>MeOHe exhibited from day 14–21 a significant decrease (p ≤ 0.05) in micronuclei assay, as well as a in single-strand breaks (p &lt; 0.05) compared against control group.</div></div><div><h3>Conclusion</h3><div>These results suggest that HlMeOHe is not cytotoxic or genotoxic maybe because of its phenolic compounds, reducing the genotoxic effect induced by V.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127677"},"PeriodicalIF":3.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing coral photosynthesis: The power of manganese-alginate gels","authors":"Gabriel Rodrigues Martins Moreira ,&nbsp;João Manoel De Lima Júnior ,&nbsp;Cassiana Seimi Nomura ,&nbsp;Jany Hellen Ferreira De Jesus ,&nbsp;Emmanuelle Uher ,&nbsp;Aurélie Dufour ,&nbsp;Maria-Isabelle Marcus Do Noscimiento ,&nbsp;Renaud Grover ,&nbsp;Christophe Migon ,&nbsp;Christine Ferrier-Pagès ,&nbsp;Breno Pannia Espósito","doi":"10.1016/j.jtemb.2025.127675","DOIUrl":"10.1016/j.jtemb.2025.127675","url":null,"abstract":"<div><h3>Introduction</h3><div>Scleractinian corals rely on symbiosis with Symbiodiniaceae, a family of marine dinoflagellates, for photosynthetic products, which sustains their growth in nutrient-poor environments. Manganese (Mn) is a critical element for photosynthesis as an essential cofactor for the oxygen-evolving complex of the photosynthetic machinery. Also, Mn is an essential metal involved in antioxidant mechanisms that maintain normal cellular function in heat stressed corals. <em>Objectives</em>: This study aims to investigate the effects of different Mn species on photosynthetic efficiency in the coral <em>Stylophora pistillata</em> under thermal stress. A Mn-alginate device for controlled Mn supplementation was developed for this purpose. <em>Methods</em>: Coral nubbins were exposed to the following Mn species: manganese(II) chloride (MnCl₂), manganese(II) ethylenediamine-N,N′-disuccinate (MnEDDS), manganese(II) desferrioxamine (MnDFO), and manganese(III) citrate (MnCIT). Photosynthetic parameters were measured using fluorometry. Mn-alginate gel dishes were prepared for controlled release, and their impacts on coral health indicators were evaluated at 26 °C and 31 °C, including photosynthetic efficiency, oxygen production, and symbiont density. <em>Results</em>: Free Mn(II) (MnCl₂) and MnEDDS significantly enhanced photosynthetic efficiency. Mn-alginate effectively delivered Mn in controlled bursts. Under thermal stress, Mn supplementation improved photosynthetic activity and favored symbiont density. Mn-alginate dishes were stable in seawater and biocompatible, releasing Mn optimally at elevated temperatures. <em>Conclusion</em><strong>:</strong> Mn-alginate gel dishes are an efficient and safe method for improving coral photosynthesis and mitigating thermal stress impacts, supporting reef conservation efforts in a changing climate.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127675"},"PeriodicalIF":3.6,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overview of the effects of heavy metals on the reproductive health of males in the genus Mytilus spp.","authors":"Carmela Marinaro ,&nbsp;Rosaria Notariale ,&nbsp;Mirko Cardillo ,&nbsp;Maria Grazia Guarnieri ,&nbsp;Marco Trifuoggi ,&nbsp;Filomena Mottola ,&nbsp;Lucia Rocco ,&nbsp;Gennaro Lettieri ,&nbsp;Luigi Montano ,&nbsp;Marina Piscopo","doi":"10.1016/j.jtemb.2025.127672","DOIUrl":"10.1016/j.jtemb.2025.127672","url":null,"abstract":"<div><div>This review examines the reprotoxic effects of key heavy metals, specifically nickel, chromium, zinc, copper, mercury and cadmium, on the reproductive health of bivalve molluscs of the genus <em>Mytilus spp</em>. which serve as important bioindicators in marine ecosystems. Exposure to even sub-lethal concentrations of these metals induces oxidative stress, DNA damage and changes in chromatin structure, which are thought to affect fertility. These metals, known for their persistence and bioaccumulation, interfere with critical reproductive processes by also altering the functions of protamine-like proteins (PLs), the main nuclear basic proteins of sperm chromatin of these organisms. Moreover, Seasonal variations influence the toxicity of these metals, coinciding with critical phases of gametogenesis and amplifying their detrimental effects during reproductive cycles. The study highlights the urgent need for comprehensive biomonitoring programmes, as the sensitivity of <em>Mytilus spp</em>. to environmental contaminants provides valuable insights into the health of marine ecosystems. Given their economic importance and ecological role as filter feeders, the impact of heavy metal pollution on these bivalve molluscs goes beyond increased environmental pollution and threatens biodiversity and seafood safety. To mitigate these risks, strict pollution controls and further research into molecular toxicology are essential. Understanding this will help to develop effective strategies to conserve marine life and maintain the ecosystem benefits provided by these sentinel species.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127672"},"PeriodicalIF":3.6,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144139351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The safety assessment of cinnabar: Effects of co-administration with selenium on renal toxicity in mice","authors":"Ziyan Han ,&nbsp;Yuzhou Zhuo ,&nbsp;Zhihui Dai ,&nbsp;Xinhui Zhang ,&nbsp;Bingqian Zhu ,&nbsp;Yuyan Liu ,&nbsp;Zhuoyu Chai ,&nbsp;Jiayi Wu ,&nbsp;Lijun Chen","doi":"10.1016/j.jtemb.2025.127671","DOIUrl":"10.1016/j.jtemb.2025.127671","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;Cinnabar is a mercury-containing mineral traditionally used in Chinese medicine and can induce kidney injury via mercury toxicity. Given that cinnabar contains elements such as selenium, it is reasonable to hypothesize that its multi-element composition may regulate nephrotoxicity through intermetallic interactions. To validate this hypothesis and clarify the distinct renal toxicity between cinnabar and single mercury compounds, we compared the nephrotoxic effects of cinnabar, Zhu-Sha-An-Shen-Wan (ZSASW), mercuric sulfide (HgS), and mercuric nitrate (Hg(NO₃)₂). By co-administration with sodium selenite (Na₂SeO₃), this study related to the nephrotoxicity of cinnabar was improved from the perspective of metal-element interactions, which provided a new perspective for the safety assessment of mercury-containing medicines.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;Mice were gavaged with 0.5 % CMC-Na solution, cinnabar (50.0 and 200 mg/kg), HgS (50.0 mg/kg), Na&lt;sub&gt;2&lt;/sub&gt;SeO&lt;sub&gt;3&lt;/sub&gt; (1.00 mg/kg), cinnabar (50.0 mg/kg)+Na&lt;sub&gt;2&lt;/sub&gt;SeO&lt;sub&gt;3&lt;/sub&gt; (1.00 mg/kg), HgS (50.0 mg/kg)+Na&lt;sub&gt;2&lt;/sub&gt;SeO&lt;sub&gt;3&lt;/sub&gt; (1.00 mg/kg), ZSASW (600 mg/kg), ZSASW (600 mg/kg)+Na&lt;sub&gt;2&lt;/sub&gt;SeO&lt;sub&gt;3&lt;/sub&gt; (1.00 mg/kg) or Hg(NO&lt;sub&gt;3&lt;/sub&gt;)&lt;sub&gt;2&lt;/sub&gt; (0.900 mg/kg in Hg&lt;sup&gt;2+&lt;/sup&gt;) daily for 30 days. Renal histopathology was assessed by H&amp;E staining. Related protein expression was measured by Western blotting. Renal total Hg (THg) concentration in kidney was determined by cold vapor atomic fluorescence spectroscopy.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Result&lt;/h3&gt;&lt;div&gt;Western blotting revealed significantly decreased OAT1 and GPX4 levels in all experimental groups compared to the Control group (&lt;em&gt;P&lt;/em&gt; &lt; 0.05). NF-κB activation occurred in the Hg(NO₃)₂, Cinnabar-H, HgS, and HgS-Na₂SeO₃ groups. Hg(NO₃)₂ administration caused a significant decline in body weight growth rate (&lt;em&gt;P&lt;/em&gt; &lt; 0.001), severe renal tubular degeneration with epithelial cell swelling, and the highest renal THg concentration (773.77 % exceeding the Control group). Na₂SeO₃ alone induced inflammatory infiltration and tubular epithelial degeneration. The Cinnabar-H and HgS groups exhibited distinct renal damage (localized tubular degeneration and vascular hyaline degeneration, respectively) with elevated renal THg concentration (330.74 % and 347.95 % exceeding the Control group). The Cinnabar-L, Cinnabar-Na₂SeO₃, ZSASW, and ZSASW-Na₂SeO₃ groups maintained normal histology despite increased renal mercury content (227.46 %, 40.57 %, 67.2 %, and 556.56 % exceeding the Control group). Co-administration of Na₂SeO₃ with HgS restored OAT1 and GPX4 expression (&lt;em&gt;P&lt;/em&gt; &lt; 0.001), suppressed NF-κB activation, and minimally altered renal mercury accumulation (RSD=0.45 %). Na₂SeO₃ reduced mercury levels in the cinnabar-treated mice (57.07 % reduction) but increased accumulation in ZSASW-treated mice (292.65 % increase).&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;div&gt;This study confirms th","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127671"},"PeriodicalIF":3.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144090432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of M86 and M87 compounds against lead, cadmium, and arsenic toxicity in 2D and 3D liver cell models","authors":"Hamdi Temel ,&nbsp;Serap Yalçın Azarkan ,&nbsp;Serkan Şahin","doi":"10.1016/j.jtemb.2025.127665","DOIUrl":"10.1016/j.jtemb.2025.127665","url":null,"abstract":"<div><div>Increasing environmental pollution increases the risk of human exposure to toxic metals. Therefore, there is a need for substances to protect individuals against the harmful effects caused by toxic metals. Bu çalışma, 3-metoksi katekol bileşiğinin 1,4-fenil diboronik asitle 1:1 ve 1:2 mol ratios of 3-methoxy catechol compound with 1,4-phenyl diboronic acid (1:1 and 1:1) and 1,4-bis(4-methoxybenzo[<em>d</em>][1,3,2]dioxaborol-2-yl)phenyl)boronic acid (M86) and 1,4-bis(4-methoxybenzo[<em>d</em>][1,3,2]dioxaborol-2-yl)benzene (M87) against lead (Pb), cadmium (Cd) and arsenic (As) toxicity in THLE-2 liver cell line. The structures of synthesized compounds M86 and M87 were characterized by 1 H, 13 C NMR, LC-MS-IT-TOF, UV-Vis., FTIR. The biological activities of these compounds were evaluated by DPPH, ABTS, CUPRAC, anticholinesterase, antiurease and antithyrosinase tests. 2D and 3D cell models were used in THLE-2 cell line. The protective effects of M86 and M87 against Pb, Cd and As toxicity were examined by XTT test and ATP colorimetric method and IC50 values were determined. In antioxidant tests, it was observed that M86 and M87 exhibited high activity in ABTS, DPPH and CUPRAC tests compared to standard antioxidants ?-tocopherol (?-TOC) and butylated hydroxytoluene (BHT). Enzyme inhibition tests showed that M86 and M87 significantly suppressed acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzyme activities. These compounds were found to reverse the decrease in cell proliferation following Pb, Cd and As exposure. In conclusion, M86 and M87 have the potential to be versatile therapeutic agents that provide effective protection against metal toxicity. In the future, with the evaluation of the efficacy of these compounds in in vivo models and clinical studies, it is thought that their use against metal toxicity may be possible.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127665"},"PeriodicalIF":3.6,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of multi-metal exposure with hyperuricemia in middle-aged and older adults: A cross-sectional study in rural Guangxi, China","authors":"Qinyi Guan ,&nbsp;Kaisheng Teng ,&nbsp;Qiumei Liu ,&nbsp;Songju Wu ,&nbsp;Lei Luo ,&nbsp;Linhai Zhao ,&nbsp;Jiahui Rong ,&nbsp;Wenjia Jin ,&nbsp;Tiantian Zhang ,&nbsp;Mo Xiaoting ,&nbsp;Cai Jiansheng ,&nbsp;Zhiyong Zhang ,&nbsp;Jian Qin","doi":"10.1016/j.jtemb.2025.127670","DOIUrl":"10.1016/j.jtemb.2025.127670","url":null,"abstract":"<div><h3>Background</h3><div>Hyperuricemia constitutes a significant global public health concern, particularly among the elderly, and is associated with environmental factors such as metal exposure. In China, the predominant focus of existing research has been on urban populations, which leaves the relationship between hyperuricemia and ecological exposures in rural populations inadequately explored. This study investigates the association between hyperuricemia and exposure to multiple metals among elderly residents in rural China.</div></div><div><h3>Methods</h3><div>Cross-sectional survey data were collected from middle-aged and elderly individuals residing in Yao Autonomous County of Gongcheng, Guangxi, China, from 2018 to 2019. The analytical techniques employed included least absolute shrinkage and selection operator (LASSO) regression, binary logistic regression and Bayesian kernel machine regression (BKMR) to examine the association between exposure to multi-metal mixtures and hyperuricemia.</div></div><div><h3>Results</h3><div>Logistic regression results indicated a negative association between magnesium (OR=0.634, 95 % CI=0.476‐0.867, Q3) and hyperuricemia, while iron, nickel, strontium, molybdenum, and tin were positively associated with hyperuricemia (all P &lt; 0.05). The Bayesian kernel machine regression analysis revealed a significant overall positive correlation between hyperuricemia and plasma polymetallic mixtures, with notable contributions from Sr (PIP=0.991) and Fe (PIP=0.749), particularly when all metals were at their respective 50th percentiles. Exposure-response relationships were consistent with the logistic regression results. Plasma Sr was positively correlated with hyperuricemia at different levels of other metals (p25, p50, p75).</div></div><div><h3>Conclusion</h3><div>This study suggests that exposure to multi-metal mixtures is positively associated with hyperuricemia, with Sr and Fe being the most significant contributors.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127670"},"PeriodicalIF":3.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First record of trace elements in cetaceans stranded along the Asturias Coastline, Northwest Spain: One great diversity area of marine mammals","authors":"L. Baños-Doménech ,&nbsp;N. García-García ,&nbsp;J. Peñalver ,&nbsp;J.A. Armenteros ,&nbsp;E. Martínez-López","doi":"10.1016/j.jtemb.2025.127669","DOIUrl":"10.1016/j.jtemb.2025.127669","url":null,"abstract":"<div><div>The concentrations of cadmium (Cd), lead (Pb), arsenic (As), selenium (Se) and mercury (Hg) in the kidney, liver, brain and muscle of 20 individuals of cetaceans, stranded along the Asturias coastline between 2018 and 2020, have been studied. Species studied include fin whale (<em>Balaenoptera physalus</em>), striped dolphin (<em>Stenella coeruleoalba</em>), common dolphin (<em>Delphinus delphis</em>), bottlenose dolphin (<em>Tursiops truncatus</em>), harbour porpoise (<em>Phocoena phocoena</em>) and pygmy sperm whale. (<em>Kogia breviceps</em>). All trace elements analyzed were detected in all cetacean families. The most detected compound was Se in the kidney (90 %), closely followed by Hg in muscle (85 %), while the least detected metal was Pb in the kidney (5 %). The highest concentrations of Se (66.00 mg/kg), Hg (44.00 mg/kg) and As (2.00 mg/kg) were detected in the liver, followed by Cd (53.00 mg/kg) in the kidney and Pb (0.37 mg/kg) in the brain. The family that presented the highest concentrations of toxic metals was <em>Delphinidae</em>, compared to the family <em>Balaenopteridae,</em> which is explained by the feeding and behavioural habits of odontocetes. The concentrations found are lower than those detected in other areas of Spain and the Atlantic Ocean and lower than those considered responsible for toxic effects in cetaceans. In addition, low levels of metal contamination (IMP) were found in most samples, although in two individuals we cannot exclude effects at the renal level due to metal accumulation. The overall effect of biologically relevant variables on metal concentrations was assessed by generalised linear modelling (GLM) analysis, with tissue being the most influential variable on metal concentrations. In addition, the Hg:Se molar ratio was lower than 1 in most samples analyzed, so the detoxifying action of Se against Hg is confirmed. As it represents the first record of trace element analysis in cetaceans of this area, our study supplies useful data for the development of conservation strategies in the Cantabrian Sea, as well as provides valuable information for future research.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127669"},"PeriodicalIF":3.6,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacotherapeutic role of astringin against chromium induced nephrotoxicity via modulating TLR4/MyD88, HMGB1/RAGE and NF-κB pathway: A biochemical and pharmacokinetic approach","authors":"Han Hu ,&nbsp;Muhammad Faisal Hayat ,&nbsp;Syeda Sania Zahara ,&nbsp;Ali Akbar ,&nbsp;Khalid J. Alzahrani ,&nbsp;Khalaf F. Alsharif ,&nbsp;Fuad M. Alzahrani ,&nbsp;Ahmed Al-Emam","doi":"10.1016/j.jtemb.2025.127666","DOIUrl":"10.1016/j.jtemb.2025.127666","url":null,"abstract":"<div><h3>Background</h3><div>Chromium (Cr) is a noxious heavy metal that is reported to induce various organ damages including the kidneys. Astringin (ATN) is a polyphenolic flavonoid that demonstrates immense pharmacological potential.</div></div><div><h3>Aim</h3><div>This research was planned to assess the potential palliative efficacy of ATN against Cr induced renal toxicity via regulating biochemical and histological parameters.</div></div><div><h3>Methodology</h3><div>Thirty-two male albino (Sprague Dawley) rats were divided into four groups: the 1st group (control), 2nd group (Cr 15 mg/kg), 3rd group (Cr 15 mg/kg + ATN 10 mg/kg), and 4th (ATN 10 mg/kg) group. Gene profile was evaluated by using qRT-PCR protocol. The levels of other biochemical parameters were assessed through standard ELISA protocol as well as already reported standard assays. Histology was performed as per the basic principle of histopathology technique. The palliative role of ATN was further confirmed by molecular docking (MD) and molecular dynamic simulation (MDS) approach.</div></div><div><h3>Results</h3><div>Cr intoxication upregulated the gene expression of <em>high mobility group box1 (HMGB1), tumor necrosis factor- α (TNF-α), nuclear factor- kappa B (NF-κB), cyclooxygenase-2 (COX-2)</em>, <em>interleukin-6 (IL-6), myeloid differentiation primary response 88 (MYD88), receptor for advanced glycation end products (RAGE), toll-like receptor 4 (TLR4),</em> and <em>interleukin-1β (IL-1β)</em>. The levels of reactive oxygen species (ROS) and malondialdehyde were elevated while the activities of glutathione reductase (GSR), glutathione peroxidase (GPx), heme oxygenase-1 (HO-1), superoxide dismutase (SOD) &amp; catalase (CAT) as well as contents of glutathione (GSH) were reduced after Cr intoxication. Moreover, Cr exposure increased the levels of cystatin C, uric acid, neutrophil gelatinase-associated lipocalin (NGAL), creatinine, blood urea nitrogen (BUN), N-acetylglucosamine (NAG), kidney injury molecule-1 (KIM-1) &amp; urea while downregulating the concentrations of creatine clearance. Besides, the levels of B cell lymphoma-2 (Bcl-2) were reduced while the levels of cysteine-aspartic acid protease-9 (Caspase-9), Bcl-2–associated X protein (Bax) and cysteine-aspartic acid protease-3 (Caspase-3) were escalated after Cr intoxication. Renal tissues showed abnormal histology following the exposure to Cr. Nonetheless, ATN treatment effectively restored biochemical as well as histological impairments in renal tissues, thereby demonstrating the nephroprotective potential against Cr intoxication.</div></div><div><h3>Conclusion</h3><div>ATN therapy showed significant renal protection via suppressing oxidative stress, inflammation, apoptosis and histological damages. These findings emphasize the important role of ATN in regulating renal health via modulating TLR4/MyD88, HMGB1/RAGE and NF-κB signaling pathway.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127666"},"PeriodicalIF":3.6,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143917515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into ionic medicine: Cerium reduces the presence of reactive oxygen species and favors osteogenic over adipogenic differentiation in human mesenchymal stromal cells","authors":"F. Westhauser ,&nbsp;V. Jacobsen ,&nbsp;K. Zheng ,&nbsp;C. Merle ,&nbsp;A.R. Boccaccini ,&nbsp;T. Renkawitz ,&nbsp;E. Kunisch","doi":"10.1016/j.jtemb.2025.127668","DOIUrl":"10.1016/j.jtemb.2025.127668","url":null,"abstract":"<div><div>The guided application of metallic ions in bone tissue engineering (BTE) has recently gained popularity being described as one important example of ionic medicine (IM). BTE aims to enhance osteogenic differentiation of precursor cells like bone-marrow-derived mesenchymal stromal cells (BMSCs) and, by that, regenerate bone tissue. BMSCs however can also differentiate into adipogenic lineage. It is known that elevated levels of reactive oxygen species (ROS) stimulate BMSC towards (undesired) adipogenic differentiation. One ion, that is particularly interesting for application in IM-guided BTE is cerium (Ce) since it acts as a self-regenerating ROS-scavenger and has already been successfully incorporated in biomaterials. Ce has demonstrated pro-osteogenic, anti-adipogenic and anti-oxidative effects before, however, so far, there is no direct comparative study available that analyzes these effects on human BMSCs in one and the same setting. Therefore, in this study, the influence of Ce nitrate (CeN) on the expression of osteogenic, adipogenic and ROS-scavenging genes in BMSCs was evaluated as well as its impact on formation of an osseous extracellular matrix (ECM), lipid formation and physical ROS presence. The presence of CeN improved BMSCs viability, enhanced proliferation, and reduced ROS-levels. Furthermore, CeN suppressed adipogenesis while osteogenic differentiation and the formation and maturation of the ECM were enhanced. The presence of CeN reduced the physical presence of ROS and the gene expression patterns shifted towards an anti-oxidant profile. Ce therefore constitutes an attractive ion for application in IM-guided BTE. Further research is necessary to clarify the biological mechanisms and pathways that are involved in the Ce-mediated modulation of BMSC differentiation.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127668"},"PeriodicalIF":3.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143917516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive assessment of matrix effects in metallomics: Evaluating 27 metals in serum, heparine-plasma-, EDTA-plasma and citrate-plasma by ICP-MS analysis","authors":"Giulia Guerra ,&nbsp;Elisabetta Venturelli ,&nbsp;Alessio Polymeropoulos ,&nbsp;Francesco Segrado ,&nbsp;Daniele Morelli ,&nbsp;Sabina Rinaldi ,&nbsp;Claudia Agnoli ,&nbsp;Sabina Sieri","doi":"10.1016/j.jtemb.2025.127667","DOIUrl":"10.1016/j.jtemb.2025.127667","url":null,"abstract":"<div><h3>Background</h3><div>Metallomics analysis is essential for studying environmental metal exposure and distinguishing between healthy and diseased individuals in large-scale studies. Inductively coupled plasma mass spectrometry (ICP-MS) is widely used due to its high sensitivity and ability to analyze multiple elements across a broad concentration range.</div></div><div><h3>Aim</h3><div>Developed to address key challenges in large-scale epidemiological studies, this method specifically focuses on evaluating the performance of metals measurement in biobank blood matrices that may not be optimized for metallomics, providing key insights for biobank planning</div></div><div><h3>Methods</h3><div>An ICP-MS-based analytical method using a \"dilute and shoot\" approach was developed to target 27 elements. The study assessed the behavior of these elements in serum and different plasma types (EDTA, citrate, and heparin) based on limits of detection (LOD), limits of quantification (LOQ), precision, accuracy, and quality controls.</div></div><div><h3>Results</h3><div>Heparin plasma and serum provided the most consistent measurements, with most elements exhibiting a coefficient of variation below 15 %. Citrated and EDTA plasma displayed higher variability, likely due to contamination from collection tubes and metal-anticoagulant interactions. Among 13 certified elements, Mg, K, Fe, Cu, Se, Co, and Ni matched reference values, while Ca, Zn, Cr, Mn, and Al were lower, and Hg was higher. Of the 14 elements with indicative values, all except Cd, which was significantly lower, aligned with Seronorm™ references.</div></div><div><h3>Conclusion</h3><div>These findings highlight the impact of plasma matrices on metal measurements in biobank studies and emphasize the importance of selecting appropriate blood matrices and collection tubes to ensure accurate and reliable elemental analysis in large-scale epidemiological research.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127667"},"PeriodicalIF":3.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143911539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}