Sub-lethal toxicity effects of iron oxide nanoparticles (IONPs) on the biochemical, oxidative biomarkers, and metabolic profile in Caridina fossarum

IF 3.6 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Hamidreza Shahryari , Iman Sourinejad , Amin Gholamhosseini , Mahdi Banaee
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Abstract

Backgrounds

This study aimed to assess the toxicity effect of iron oxide nanoparticles (IONPs) on the biochemical and oxidative markers in freshwater miniature shrimp (Caridina fossarum).

Materials and methods

Based on the pre-test results, 540 shrimp were distributed into six trial groups in triplicate and exposed to sub-lethal concentrations of Fe3O4 nanoparticles at 0.0, 40, 80, 120, 160, and 320 µg L−1 for 14 days. Next, biochemical parameters and oxidative biomarkers were measured.

Results

The results showed that exposure to 120 µg L⁻¹ ≤ of IONPs significantly increased aspartate aminotransferase activity in C. fossarum. Alanine aminotransferase activity showed a significant increase at 320 µg L⁻¹ . Similarly, alkaline phosphatase activity was meaningfully elevated at 160 and 320 µg L⁻¹ . Lactate dehydrogenase activity notably increased at 120 and 320 µg L⁻¹ of IONPs. IONPs at ≥ 80 µg L⁻¹ significantly reduced gamma-glutamyl transpeptidase and butyrylcholinesterase activities. Cholesterol and triglyceride levels significantly increased at 320 µg L⁻¹ . Exposure to 80 µg L⁻¹ ≤ of IONPs significantly increased superoxide dismutase, catalase, glutathione reductase, and glutathione peroxidase activities in C. fossarum. Moreover, total antioxidant capacity and malondialdehyde content increased considerably in shrimp exposed to different concentrations of IONPs. Exposure to IONPs could induce oxidative stress, disrupt protein structures, oxidize sulfur-containing and essential aromatic and aliphatic amino acids, impair nucleic acid stability, and change lipid metabolism and membrane integrity.

Conclusion

In conclusion, significant changes in biochemical parameters, oxidative biomarkers, and metabolic profile disruptions in C. fossarum exposed to sub-lethal concentrations of IONPs indicated cellular damage and oxidative stress.
氧化铁纳米颗粒(IONPs)对Caridina fossarum生物化学、氧化生物标志物和代谢谱的亚致死毒性影响
本研究旨在探讨氧化铁纳米颗粒(IONPs)对淡水小对虾(Caridina fossarum)生化和氧化标志物的毒性作用。材料与方法根据预试结果,将540只对虾分为6个试验组,每组3次,分别暴露于浓度为0.0、40、80、120、160和320 µg L−1的亚致死浓度的Fe3O4纳米颗粒中14天。接下来,测量生化参数和氧化生物标志物。结果暴露在120 µg L⁻¹ ≤的IONPs环境下,可显著提高弓形虫的天冬氨酸转氨酶活性。丙氨酸转氨酶活性显著增加320 µg L⁻¹ 。同样,碱性磷酸酶活性在160和320 µg L⁻¹ 时显著升高。乳酸脱氢酶活性在120和320 µg L⁻¹ 时显著增加。≥ 80 µg L⁻¹ 的IONPs显著降低γ -谷氨酰转肽酶和丁酰胆碱酯酶活性。胆固醇和甘油三酯水平显著增加到320 µg L⁻¹ 。暴露于80 µg L⁻¹ ≤的IONPs显著增加了C. fossarum的超氧化物歧化酶、过氧化氢酶、谷胱甘肽还原酶和谷胱甘肽过氧化物酶的活性。此外,暴露于不同浓度的IONPs下,虾的总抗氧化能力和丙二醛含量显著增加。暴露于IONPs可诱导氧化应激,破坏蛋白质结构,氧化含硫和必需芳香和脂肪氨基酸,损害核酸稳定性,改变脂质代谢和膜完整性。结论暴露于亚致死浓度的IONPs下,弓形虫的生化参数、氧化生物标志物和代谢谱发生显著变化,表明细胞损伤和氧化应激。
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来源期刊
CiteScore
6.60
自引率
2.90%
发文量
202
审稿时长
85 days
期刊介绍: The journal provides the reader with a thorough description of theoretical and applied aspects of trace elements in medicine and biology and is devoted to the advancement of scientific knowledge about trace elements and trace element species. Trace elements play essential roles in the maintenance of physiological processes. During the last decades there has been a great deal of scientific investigation about the function and binding of trace elements. The Journal of Trace Elements in Medicine and Biology focuses on the description and dissemination of scientific results concerning the role of trace elements with respect to their mode of action in health and disease and nutritional importance. Progress in the knowledge of the biological role of trace elements depends, however, on advances in trace elements chemistry. Thus the Journal of Trace Elements in Medicine and Biology will include only those papers that base their results on proven analytical methods. Also, we only publish those articles in which the quality assurance regarding the execution of experiments and achievement of results is guaranteed.
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