Journal of Trace Elements in Medicine and Biology最新文献

{"title":"Selenium biomarkers and miR‐7‐5p in overweight/obese women","authors":"Higor Paiva de Mendonça Alves ,&nbsp;Graziela Biude Silva Duarte ,&nbsp;Adriano Carlos de Souza Junior ,&nbsp;Leonam da Silva Pereira Batista ,&nbsp;Marcelo Macedo Rogero ,&nbsp;Fernando Barbosa Jr ,&nbsp;Silvia Maria Franciscato Cozzolino ,&nbsp;Raquel Costa Silva Dantas-Komatsu ,&nbsp;Karina Zaira Silva Marinho Costa ,&nbsp;Bruna Zavarize Reis","doi":"10.1016/j.jtemb.2024.127499","DOIUrl":"10.1016/j.jtemb.2024.127499","url":null,"abstract":"<div><h3>Introduction</h3><p>Chronic low-grade inflammation and oxidative stress are pivotal contributors to the metabolic complications associated with obesity. Selenoprotein P (SELENOP) and glutathione peroxidase 1 (GPx1) are selenoproteins involved in the reduction of reactive oxygen species and pro-inflammatory cytokines levels. Nutritional epigenomics revealed the interaction of microRNAs and nutrients with an important impact on metabolic pathways involved in obesity. However, the knowledge regarding the influence of microRNA on selenium biomarkers and its impact on metabolic pathways related to obesity remains scarce. Thus, the aim of this study was to investigate the association of plasma miR‐7‐5p expression with selenium and inflammatory biomarkers in women with overweight/obesity.</p></div><div><h3>Material and methods</h3><p>Anthropometric evaluations were performed and blood samples were collected for the analysis of fasting glucose, insulin, inflammatory and selenium biomarkers, and miR-7–5p expression in 54 women with overweight/obesity. Gene expression of <em>SELENOP</em> and <em>GPX1</em> were evaluated in peripheral mononuclear blood cells.</p></div><div><h3>Results</h3><p>This study observed a negative correlation between SELENOP levels and miR-7–5p (rho = −0.350; p = 0.018). Additionally, it was observed that body fat (OR = 0.737; p = 0.011), age (OR = 1.214; p = 0.007), and miR‐7‐5p (OR = 0.990; p = 0.015) emerged as significant predictors of SELENOP levels.</p></div><div><h3>Conclusions</h3><p>In conclusion, we observed a significant inverse association between miR‐7‐5p expression and SELENOP concentration in overweight/obese women, suggesting that age and percentage of body fat are also associated.</p></div><div><h3>Trial registration number</h3><p>Brazilian Registry of Clinical Trials (ReBEC) number RBR-2nfy5q</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"86 ","pages":"Article 127499"},"PeriodicalIF":3.6,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141842838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iodine and selenium: Dietary sources and nutritional status of the population of the Kurdistan Region in Northern Iraq","authors":"A.B. Karim ,&nbsp;S.D. Young ,&nbsp;K.A.M. Hawrami ,&nbsp;E.H. Bailey","doi":"10.1016/j.jtemb.2024.127495","DOIUrl":"10.1016/j.jtemb.2024.127495","url":null,"abstract":"<div><h3>Aim</h3><p>The primary aim of this study was to determine the selenium (Se) and iodine (I) food concentrations and dietary intake of the population living in the Kurdish controlled region of northern Iraq. We also assessed the extent to which iodised salt contributes to dietary iodine intake.</p></div><div><h3>Methodology</h3><p>Foods and samples of salt and drinking water were analysed, including 300 crops samples from 40 local farms. The results, supplemented by food composition data, were used to assess dietary Se and I intake for 410 volunteers using a semi-quantitative food questionnaire. To directly investigate the nutritional status of individuals, urine samples were also collected from participants.</p></div><div><h3>Results</h3><p>Selenium intake was mainly supplied by protein and cereal sources. Calculated median dietary intake of Se was 62.7 µg d⁻¹ (mean = 66.3 µg d⁻¹) with c. 72 % of participants meeting or exceeding dietary reference intake recommendations for age. Median dietary intake of I, excluding salt consumption, was 94.6 µg d⁻¹ (mean 100.2 µg d⁻¹), increasing to 607.2 µg d⁻¹ when salt (of which &gt;90 % was iodized) was included. Salt intake was estimated to be c.13.5 g d⁻¹ (5400 mg Na d⁻¹) which greatly exceeds WHO recommended intake (&lt; 2000 mg d⁻¹ of Na). Urine iodine concentrations indicated that 98 % of school aged children had excessive iodine intake (≥300 µg L⁻¹) and 80–90 % of all study participants had above average or excessive iodine intake (≥200 µg L⁻¹).</p></div><div><h3>Conclusions</h3><p>Poultry and rice are the main sources of dietary Se to this population but around a third of children receive an inadequate Se intake. Fresh fruit and vegetables are the main sources of dietary I, but consumption of local foods cannot supply adequate I without iodised salt supplementation. Consumption of iodized salt well above recommended amounts is supplying this population with substantial iodine intake. Interventions to reduce salt intake would help to limit excessive iodine intake whilst also reducing cardio-vascular risks from Na consumption.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"85 ","pages":"Article 127495"},"PeriodicalIF":3.6,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0946672X24001159/pdfft?md5=a0ac8945f5e02225121e657d4a9e0b00&pid=1-s2.0-S0946672X24001159-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141629967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blood levels of metallic chemical element exposure patterns and associated factors in a population living in an Industrial District in Brazil","authors":"Maria Cristina Ferreira Lemos ,&nbsp;Rosalina Jorge Koifman ,&nbsp;Fernando Barbosa Jr ,&nbsp;Valéria Saraceni ,&nbsp;Evanelza Quadros ,&nbsp;Rafael do Nascimento Pinheiro ,&nbsp;Vanessa Cristina de Oliveira Souza ,&nbsp;Rafaela Soares Senra da Costa ,&nbsp;Ilce Ferreira da Silva","doi":"10.1016/j.jtemb.2024.127498","DOIUrl":"10.1016/j.jtemb.2024.127498","url":null,"abstract":"<div><p>AIM:to estimate the level of metallic chemical elements in the population living in the Steel company vicinity in Santa Cruz, Rio de Janeiro, Brazil; and estimate the association between exposure to the Steel company and the blood metals concentrations patterns.METHOD:A cross-sectional study was carried out on 463 individuals aged 18+ years old residing 1+ years in the Steel company vicinity. Mg, Be, Co, Ba, Ni, Cd, Al, and Pb were assessed in blood by DRC-ICP-MS. Metallic chemical element concentration patterns were obtained by exploratory factor analysis in the studied population. Exposure was set as the distance (Km) from each participant's residence to the Steel company in Santa Cruz, georeferenced by GPS. The outcome was set as the positive factor loadings in the factor analysis, including Mg and Be (Factor-1), Co, Ba, and Ni (Factor-2), Cd, Al, and Pb (Factor-4). Crude and adjusted OR, and their respective 95 %CI, were estimated to explore associations between independent variables and the exposures to metallic elements positively associated with the factors using polychotomous logistic regression.RESULTS:A reduction of 19 % was found between each km distance from the residence and the Steel company and P₅₀ concentration of Cd, Al, and Pb (OR_P50=0.81; 95 %CI:0.67–0.97), after adjusting by age, sex, and smoking. No statistically significant associations were observed for the distance from residences and the Steel company, after adjusting for age, gender, having a domestic vegetable garden and chewing gum for Mg and Be concentrations (Factor-1) (OR_P50=0.84; 95 %CI:0.70–1.01; OR_P75=1.10; 95 %CI:0.91–1.34); nor for Co, Ba and Ni (Factor-2) blood concentrations(OR_P50=1.10; 95 %CI:0.91–1.33; OR_P75=1.03; 95 %CI:0.84–1.26), in the adjusted analysis.CONCLUSIONS: For each Km distance from residences to the Steel company, a 19 % reduction in the risk of Cd, Al, and Pb blood concentration was observed in the population living in Santa Cruz, Rio de Janeiro, Brazil.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"85 ","pages":"Article 127498"},"PeriodicalIF":3.6,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141637525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urinary concentrations of mineral elements and their predictors in pregnant women in Jinan, China","authors":"Xiaohui Guo ,&nbsp;Jiayi Song ,&nbsp;Xiang Wang ,&nbsp;Qichen Huang ,&nbsp;Chuanling Wei ,&nbsp;Yujie Yang ,&nbsp;Nan Li ,&nbsp;Shuang Cheng ,&nbsp;Jiao Li ,&nbsp;Qi Li ,&nbsp;Ju Wang","doi":"10.1016/j.jtemb.2024.127496","DOIUrl":"10.1016/j.jtemb.2024.127496","url":null,"abstract":"<div><h3>Background</h3><p>The essential mineral elements play important roles in proper growth, development and maintenance of physiological homeostasis of an organism. Women are at greater risk of mineral deficiency during pregnancy. However, the predictors of mineral element levels in pregnant women remain unclear. This study was conducted to determine the urinary levels of calcium (Ca), iron (Fe), copper (Cu), manganese (Mn) and selenium (Se) in women during early pregnancy and to explore the predictors of urinary exposure to each mineral element and high co-exposure to mineral element mixture.</p></div><div><h3>Methods</h3><p>298 pregnant women in first trimester were recruited when they attended antenatal care in a hospital in Jinan, Shandong Province, China. We collected their spot urine samples and questionnaire data on their sociodemographic characteristics, lifestyle habits, food and dietary supplement intake, and residential environment. The concentrations of Ca, Fe, Cu, Mn and Se in all urine samples were measured. LASSO regression, multiple linear regression and binary logistic regression were used to analyze the predictors affecting mineral element levels.</p></div><div><h3>Results</h3><p>The geometric means of creatinine-corrected Ca, Fe, Cu, Mn and Se concentrations were 99.37 mg/g, 1.75 µg/g, 8.97 µg/g, 0.16 µg/g and 16.83 µg/g creatinine, respectively. Factors that influenced the concentrations of individual mineral element were as follows: (1) Se and Ca concentrations increased with maternal age; (2) women taking tap water as family drinking water had higher Ca levels and those taking polyunsaturated fatty acids intermittently had higher Cu levels; (3) Fe was adversely related to consumption frequency of barbecued foods; (4) Pregnant women with more frequent consumption of shellfish/shrimp/crab and living near green spaces or parks had higher Mn exposure, and those with higher frequency of meat consumption had lower Mn exposure. In addition, maternal age and the frequency of egg consumption were associated with odds of exposure to a mixture of high Ca, Fe, Cu and Se.</p></div><div><h3>Conclusions</h3><p>The pregnant women in this study had comparable concentrations of urinary Cu and Se but lower concentrations of Ca, Fe and Mn compared with those in other areas. Predictors of urinary mineral elements included maternal age (Se and Ca), type of domestic drinking water (Ca), consumption frequency of barbecued food (Fe), polyunsaturated fatty acid use (Cu), the presence of urban green spaces or parks near the home and frequency of meat and shellfish/shrimp/crab intake (Mn). Moreover, maternal age and egg consumption frequency were significant predictors of high-level co-exposure to urinary Ca, Fe, Cu and Se.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"85 ","pages":"Article 127496"},"PeriodicalIF":3.6,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141716648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of the vitamin D and resveratrol administration on the stiffness and elasticity of T2DM rat aorta associated with the trace element and mineral levels","authors":"Duygu Aydemir ,&nbsp;Naveed Salman ,&nbsp;Umut Kerimzade ,&nbsp;Merve Anapali-Aykac ,&nbsp;Turgut Ulutin ,&nbsp;Evrim Komurcu-Bayrak ,&nbsp;Fatma Kaya-Dagistanli ,&nbsp;B. Erdem Alaca ,&nbsp;Nuriye Nuray Ulusu","doi":"10.1016/j.jtemb.2024.127497","DOIUrl":"10.1016/j.jtemb.2024.127497","url":null,"abstract":"<div><h3>Background</h3><p>Type 2 diabetes mellitus (T2DM) is directly associated with increased aortic stiffness, reduced aortic elasticity, and aortic dissection, which are independent risk factors for cardiovascular death. Since Vit D and resveratrol have been reported due to their cardioprotective effects, in this study, we aim to evaluate the impact of Vit D and resveratrol treatment alone or in combination on the aortic health associated with trace element and mineral levels in a high-fructose diet/streptozotocin-induced T2DM model.</p></div><div><h3>Methods</h3><p>We investigated biomechanical changes of the aorta samples via a custom-built stretcher, where trace element and mineral levels in aorta samples were determined via inductively coupled plasma mass spectrometry (ICP-MS) following acidic microwave digestion.</p></div><div><h3>Results</h3><p>Vitamin D treatment ameliorated the adverse effects of T2DM on aortic stiffness, aortic elasticity, and relaxation modulus in diabetic rats. Trace element and mineral levels correlated with cardiovascular homeostasis, including Fe, Cu, Zn, Se, and Na, have been regulated upon Vit D treatment in diabetic and healthy rats. On the other hand, resveratrol treatment alone or in combination with Vit D did not show any positive effects on biomechanical properties and trace element metabolism of diabetic or healthy rats, according to our data.</p></div><div><h3>Conclusion</h3><p>Vit D can be used in T2DM patients to protect their cardiovascular health and should be considered a promising targeted therapy approach via nanoparticles to target cardiovascular diseases in the future.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"86 ","pages":"Article 127497"},"PeriodicalIF":3.6,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141714726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lead in umbilical cord blood and associated factors in Casablanca Morocco: A preliminary results","authors":"Latifa Rouzi ,&nbsp;Hecham El-Hamri ,&nbsp;Abha Cherkani-Hassani ,&nbsp;Nadia Benbounou ,&nbsp;Khalid El Kari ,&nbsp;Said Bouhya ,&nbsp;Hassan Aguenaou ,&nbsp;Zineb Jouhadi ,&nbsp;Mohammed Fekhaoui","doi":"10.1016/j.jtemb.2024.127494","DOIUrl":"10.1016/j.jtemb.2024.127494","url":null,"abstract":"<div><h3>Background</h3><p>Lead is the most common toxic metal to which Moroccans are exposed. Given the susceptibility of the fetus to lead, it is crucial to assess prenatal lead exposure. However, in Morocco, no study has assessed prenatal exposure to lead. The main goals of the present study are to determine lead concentration in umbilical cord blood and identify risk factors for prenatal lead exposure in Casablanca, Morocco.</p></div><div><h3>Methods</h3><p>To achieve these purposes, 87 cord blood lead samples were collected from mothers-baby pairs from January to December 2019. Indeed, a structured questionnaire was used to collect socio-demographic characteristics, obstetric data, leisure, cultural habits, and environmental information. Cord blood lead level was analyzed by inductively coupled plasma-mass spectrometry.</p></div><div><h3>Results</h3><p>The median lead concentration was 4.902 µg/dl with a minimum and maximum of 0.833 µg/dl and 23.593 µg/dl, respectively. A high proportion of the newborns (65.52 %) had cord blood lead levels above the Centers for Disease Control (CDC) allowable threshold limit (3.5 µg/dl). Statistical analysis was performed to assess the association between blood levels and the above factors. Lead levels in cord blood were significantly associated with the maternal educational level, anemia history, delivery mode, passive smoking during pregnancy as well as with the frequency of consumption of tea.</p></div><div><h3>Conclusion</h3><p>This study provides the first data on lead levels in newborns. Cord blood lead levels were high in the majority of the participants, these results reinforce the need to establish health surveillance programs in Morocco.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"85 ","pages":"Article 127494"},"PeriodicalIF":3.6,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141637526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends of hair Hg accumulation in reproductive-age women living in Central Russia and the calculated costs of Hg-induced IQ loss in the period between 2005 and 2021","authors":"Anatoly V. Skalny ,&nbsp;Tatiana V. Korobeinikova ,&nbsp;Anatoly A. Kirichuk ,&nbsp;Michael Aschner ,&nbsp;Monica M.B. Paoliello ,&nbsp;Fernando Barbosa Jr ,&nbsp;Marcelo Farina ,&nbsp;Alexey A. Tinkov","doi":"10.1016/j.jtemb.2024.127493","DOIUrl":"10.1016/j.jtemb.2024.127493","url":null,"abstract":"<div><p>The objective of the present study was to retrospectively evaluate hair mercury (Hg) content in reproductive-age women living in Central Russia (Moscow and Moscow region), and to calculate the potential costs of the potential Hg-induced IQ loss in a hypothetical national birth cohort.</p></div><div><h3>Materials and methods</h3><p>A total of 36,263 occupationally non-exposed women aged between 20 and 40 years living in Moscow (n = 30,626) or Moscow region (n = 5637) in the period between 2005 and 2021 participated in this study. Hair Hg content was evaluated with inductively coupled plasma-mass spectrometry (ICP-MS). Hair Hg levels in reproductive-age women were used for assessment of the potential IQ loss and its costs.</p></div><div><h3>Results</h3><p>The results demonstrate that hair Hg content in the periods between 2010 and 2015, and 2016–2021 was significantly lower than that in 2005–2009 by 26 % and 51 %, respectively. The highest hair Hg level was observed in women in 2005 (0.855 µg/g), being more than 2.5-fold higher than the lowest value observed in 2020 (0.328 µg/g). Multiple regression analysis revealed a significant inverse association between the year of analysis and hair Hg content (β = −0.288; p &lt; 0.001). The calculations demonstrate that in 2005 the costs of IQ loss in children exceeded 1.0 (1.6) billion USD, whereas in 2020 the costs of IQ loss accounted to approximately 0.15 (0.28) billion USD.</p></div><div><h3>Conclusion</h3><p>Taken together, our data demonstrate that Hg accumulation in reproductive-age women reduced significantly in Russia from 2005 to 2021 resulting in predicted economic benefits by decreasing the costs of Hg-induced IQ loss.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"85 ","pages":"Article 127493"},"PeriodicalIF":3.6,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141581937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low expression of selenoprotein S induces oxidative damage in cartilages","authors":"Yixin Cui ,&nbsp;Yucheng Liao ,&nbsp;Yonghui Chen ,&nbsp;Xu Zhao ,&nbsp;Yi Zhang ,&nbsp;Hui Wang ,&nbsp;Lian Li ,&nbsp;Xinhe Zhang ,&nbsp;Kunpan Chen ,&nbsp;Mingzhao Jia ,&nbsp;Jing Tian ,&nbsp;Xingran Ruan ,&nbsp;Yawen Shi ,&nbsp;Pinglin Yang ,&nbsp;Jinghong Chen","doi":"10.1016/j.jtemb.2024.127492","DOIUrl":"10.1016/j.jtemb.2024.127492","url":null,"abstract":"<div><p>Low levels of the indispensable trace element selenium (Se) can cause oxidative stress and disrupt environmental homeostasis in humans and animals. Selenoprotein S (Selenos), of which Se is a key component, is a member of the selenoprotein family involved in various biological processes. This study aimed to investigate whether low-level SELENOS gene expression can induce oxidative stress and decrease the antioxidative capacity of chondrocytes. Compared with control cells, SELENOS-knockdown ATDC5 cells showed substantially higher dihydroethidium, reactive oxygen species and malondialdehyde levels, and lower superoxide dismutase (SOD) expression. Knockout of the gene in C57BL/6 mice increased the 8-hydroxy-2-deoxyguanosine level considerably and decreased SOD expression in cartilages relative to the levels in wild-type mice. The results showed that the increased nuclear factor erythroid 2-related factor 2/heme oxygenase-1 signaling mediated by low-level SELENOS expression was involved in oxidative damage. The proliferative zone of the cartilage growth plate of SELENOS-knockout mice was shortened, suggesting cartilage differentiation dysfunction. In conclusion, this study confirmed that low-level Selenos expression plays a role in oxidative stress in cartilages.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"85 ","pages":"Article 127492"},"PeriodicalIF":3.6,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141536286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Testicular toxicity in cisplatin-treated Wistar rats is mitigated by Daflon and associated with modulation of Nrf2/HO-1 and TLR4/NF-kB signaling","authors":"Roland Eghoghosoa Akhigbe ,&nbsp;Olayinka Emmanuel Adelowo ,&nbsp;Esther Olamide Ajani ,&nbsp;Rachael Ibukun Oyesetan ,&nbsp;David Damola Oladapo ,&nbsp;Tunmise Maryanne Akhigbe","doi":"10.1016/j.jtemb.2024.127489","DOIUrl":"10.1016/j.jtemb.2024.127489","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;p&gt;Testicular toxicity is a complication of cisplatin therapy and it limits its use. Since cisplatin-induced testicular damage is mediated by inflammation and oxidative stress, evaluation of the protective role of antioxidant and anti-inflammatory molecules such as micronized purified flavonoid fraction (Daflon®) is pertinent. Aim: Therefore, this study investigated the mitigating effect of daflon against cisplatin-induced testicular toxicity. Also, the impact of daflon on Nrf2/HO-1 and TLR4/NF-kB pathways, which are key pathways in cisplatin toxicity, was explored. Materials and methods: After 2 weeks of acclimatization, 20 male albino Wistar rats were allotted at random into 4 equal groups; control, daflon-treated, cisplatin-treated, and cisplatin+daflon-treated. Results: Daflon significantly restored cisplatin-induced reductions in body weight (112.20±9.01 vs. 129.60±5.68, &lt;em&gt;P&lt;/em&gt;= 0.0175), body weight gain (-39.80±9.52 vs. −16.80±16.53, &lt;em&gt;P&lt;/em&gt;= 0.0154), and testicular weight (1.69±0.08 vs. 1.95±0.13, &lt;em&gt;P&lt;/em&gt;= 0.0980) and alterations in testicular histology. In addition, daflon abrogated cisplatin-induced rise in testicular CK (55.53±2.77 vs. 37.40±3.29, &lt;em&gt;P&lt;&lt;/em&gt; 0.0001) and LDH (74.52±3.20 vs. 65.89±2.08, &lt;em&gt;P&lt;/em&gt;= 0.0009) activities, and lactate content (180.50±4.19 vs. 166.20±2.78, &lt;em&gt;P&lt;/em&gt;&lt; 0.0001). Also, daflon alleviated cisplatin-induced suppression of GnRH (5.09±0.60 vs. 10.17±0.51, &lt;em&gt;P&lt;/em&gt;&lt; 0.0001), LH (1.33±0.07 vs. 2.77±0.13, &lt;em&gt;P&lt;/em&gt;&lt; 0.0001), FSH (0.51±0.10 vs. 1.82±0.09, &lt;em&gt;P&lt;/em&gt;&lt; 0.0001), and testosterone (2.39±0.11 vs. 4.70±0.33, &lt;em&gt;P&lt;/em&gt;&lt; 0.001) as well as lowered sperm quality. More so, daflon attenuated cisplatin-induced testicular oxidative stress, inflammation, and apoptosis evidenced by daflon-driven suppression of MDA (14.16±0.66 vs. 9.22±0.52, &lt;em&gt;P&lt;/em&gt;&lt; 0.0001), TNF-α (79.42±5.66 vs. 54.13±3.56, &lt;em&gt;P&lt;/em&gt;&lt; 0.0001), IL-1β (8.63±0.41 vs. 3.37±0.43, &lt;em&gt;P&lt;/em&gt;&lt; 0.0001), IL-6 (6.87±0.48 vs. 3.67±0.32, &lt;em&gt;P&lt;/em&gt;&lt; 0.0001), and caspase 3 activity (4.20±0.26 vs. 0.72±0.23, &lt;em&gt;P&lt;/em&gt;&lt; 0.0001) and DNA fragmentation (34.60±3.05 vs. 17.20±3.19, &lt;em&gt;P&lt;/em&gt;&lt; 0.0001), and upregulation of GSH level (0.07±0.03 vs. 0.36±0.03, &lt;em&gt;P&lt;/em&gt;&lt; 0.0001), and GPx (5.96±0.46 vs. 11.88±1.05, &lt;em&gt;P&lt;/em&gt;&lt; 0.0001), GST (5.16±0.71 vs. 11.50±0.81, &lt;em&gt;P&lt;/em&gt;&lt; 0.0001), SOD (1.29±0.15 vs. 2.81±0.29, &lt;em&gt;P&lt;/em&gt;&lt; 0.0001), and catalase activities (6.18±0.69 vs. 10.71±0.74, &lt;em&gt;P&lt;/em&gt;&lt; 0.0001). Furthermore, daflon upregulated testicular Nrf2 expression (40.25±2.65 vs. 66.62±4.01, &lt;em&gt;P&lt;/em&gt;&lt; 0.0001) and HO-1 (4.18±0.56 vs. 8.79±0.55, &lt;em&gt;P&lt;/em&gt;&lt; 0.0001) activity but downregulated TLR4 (11.63±0.89 vs. 7.23±0.43, &lt;em&gt;P&lt;/em&gt;&lt; 0.0001) and NF-kB levels (113.20±3.36 vs. 78.22±3.90, &lt;em&gt;P&lt;/em&gt;&lt; 0.0001) in cisplatin-treated rats. Conclusion: Collectively, the ameliorative effect of daflon on cisplatin-induced testicular toxicity is associated with inh","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"85 ","pages":"Article 127489"},"PeriodicalIF":3.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141474057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of essential and toxic trace element levels in erythrocytes of hemodialysis patients with end-stage renal disease","authors":"Monika Rajkowska-Myśliwiec ,&nbsp;Małgorzata Szczuko ,&nbsp;Agata Witczak ,&nbsp;Małgorzata Kaczkan ,&nbsp;Sylwia Małgorzewicz","doi":"10.1016/j.jtemb.2024.127491","DOIUrl":"10.1016/j.jtemb.2024.127491","url":null,"abstract":"<div><h3>Background</h3><p>Chronic kidney disease (CKD) is a global public health problem, resulting in end-stage kidney disease, cardiovascular disease, and premature death.</p></div><div><h3>Aim</h3><p>The aim of the study was to determine the profile of essential and toxic trace elements in erythrocytes of patients with end-stage renal disease (ESRD) and their relationship with selected anthropometric and biochemical parameters.</p></div><div><h3>Methods</h3><p>The present study compared the profiles of trace elements, including toxic sub-stances, in the erythrocytes of 80 hemodialysis patients with CKD with 40 healthy subjects. All patients had stage 5 CKD. The levels of Cd and Pb were determined by graphite furnace atomic absorption spectrometry and levels of Fe, Mn, Zn, Cu Cr, Ni, and Li by inductively coupled plasma atomic emission spectrometry.</p></div><div><h3>Results</h3><p>The ESRD patients demonstrated significantly lower Fe and Zn concentrations and significantly higher Mn and Li and toxic Pb and Cd concentrations in erythrocytes compared to those of the healthy controls. Negative correlations were observed, among others, between the concentrations of Cu, Li, and creatinine; Cu and phosphates; Mn, Pb, and transferrin saturation while positive correlations were noted between Cu, Cr, and transferrin and Pb, Cr, and the normalized protein catabolism rate.</p></div><div><h3>Conclusions</h3><p>The higher concentrations of toxic elements present in the erythrocytes of CKD patients might have resulted from the reduced ability of the kidneys to excrete them. Moreover, differences in the concentrations of essential elements (Fe, Mn, Zn) between the two groups indicated that their resorption in the kidneys of CKD patients was impaired. Patients with CKD might benefit from interventions intended to reduce high, toxic concentrations of Pb and Cd and Li and Mn as an alternative supportive treatment. Iron and zinc supplementation should be a component for the treatment of anemia in CKD patients.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"85 ","pages":"Article 127491"},"PeriodicalIF":3.6,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0946672X24001111/pdfft?md5=087a0b444cec7933e9f3db10b16fe24f&pid=1-s2.0-S0946672X24001111-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141474056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}