Journal of Trace Elements in Medicine and Biology最新文献

{"title":"Quercetin-conjugated gold-decorated simonkolleite nanohybrids: Insights into oxidative stress and antibacterial activity","authors":"Allisson Benatti Justino ,&nbsp;Phelipe Augusto Borba Martins Peres ,&nbsp;Natieli Saito ,&nbsp;Vinicius Prado Bittar ,&nbsp;Iasmin Aparecida Cunha Araújo ,&nbsp;Arthur Franco Demétrio ,&nbsp;Lucas Correia Peres ,&nbsp;Neide Maria da Silva ,&nbsp;Foued Salmen Espindola ,&nbsp;Anielle Christine Almeida Silva","doi":"10.1016/j.jtemb.2025.127709","DOIUrl":"10.1016/j.jtemb.2025.127709","url":null,"abstract":"<div><div>Engineered nanomaterials (ENMs) based on Simonkolleite (SK) exhibit promising theranostic applications. The functionalization of ENMs with polyphenols holds the potential to facilitate manipulation and enhance stability, activity, and bioaccessibility. In this study, nanohybrids of SK composed of gold (Au) and quercetin were synthesized. The successful synthesis was confirmed through ultraviolet-visible, Fourier-transform infrared, Raman spectroscopy, and scanning electron microscopy. The investigation aimed to elucidate the modulation of antioxidant and oxidative parameters by these ENMs and assess their cytotoxicity and antimicrobial properties. SK nanohybrids incorporating quercetin demonstrated antioxidant properties through ORAC and DPPH evaluations, confirming the presence of the biomolecule in the inorganic matrix. SK induced ROS production and lipid peroxidation, while functionalization with Au and quercetin normalized these oxidative parameters. However, quercetin-conjugated SK promoted protein oxidation. SK and its nanocomposites diminished the viability of fibroblasts, whereas Au-doped SK and its hybrid with quercetin were cytotoxic for macrophages only at elevated concentrations (≥ 250 µg/mL). Additionally, the quercetin-conjugated ENMs exhibited antibacterial efficacy against <em>Salmonella</em> strains, particularly targeting <em>S.</em> Gallinarum and <em>S.</em> Enteritidis. This study demonstrated how SK nanoparticles, when functionalized with quercetin, can modulate antioxidant and oxidative mechanisms, while also showcasing their notable antibacterial activity against <em>Salmonella</em> strains. These findings underscore the significant role of such criteria in understanding the primary biological responses induced by ZnO-based ENMs.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"91 ","pages":"Article 127709"},"PeriodicalIF":3.6,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144769480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supplementing advanced chelated trace minerals beyond recommended inorganic levels improve growth performance and physiological stress responses in broiler chickens under heat stress","authors":"Taher Mohammadizad ,&nbsp;Kamran Taherpour ,&nbsp;Hossein Ali Ghasemi ,&nbsp;Hassan Shirzadi ,&nbsp;Fatemeh Tavakolinasab ,&nbsp;Mohammad Hassan Nazaran","doi":"10.1016/j.jtemb.2025.127708","DOIUrl":"10.1016/j.jtemb.2025.127708","url":null,"abstract":"<div><h3>Background</h3><div>Organic trace minerals (TM) offer potential benefits for poultry health and productivity; however, their effectiveness under heat stress (HS) conditions is not well characterized. This study aimed to evaluate the effects of supplementing broiler diets with an advanced chelate-based trace mineral complex (ATMC) under cyclic HS conditions.</div></div><div><h3>Methods</h3><div>A total of 600 one-day-old Ross 308 broilers were randomly assigned to five dietary treatment groups for a 42-day study: (1) TC, which received a basal diet supplemented with the commercial levels of inorganic trace minerals (ITM) under thermoneutral conditions; (2) HSC, which consisted of a basal diet with the commercial ITM levels under heat stress; (3) ITM+ATMC12.5; (4) ITM+ATMC25, where diets were supplemented with 12.5 % and 25 % additional TM from ATMC, respectively, under HS; and (5) ITM125, which included a diet with 125 % of the recommended ITM levels under HS.</div></div><div><h3>Results</h3><div>The ITM+ATMC25 group exhibited significantly higher weight gain, European Production Efficiency Index, serum T₄ concentration, and duodenal villus height compared to the HSC group, with results comparable to the TC group. Additionally, feed conversion ratio, mortality rate, abdominal fat, liver weight, heterophil-to-lymphocyte ratio, alkaline phosphatase activity, and corticosterone concentration were lower in the TC and ITM+ATMC25 groups compared to the HSC group. The expression levels of heat shock protein (HSP) 70 in the liver and hypothalamus were lower in the TC and ITM+ATMC25 groups, indicating improved stress resilience. Furthermore, hepatic HSP90 expression was downregulated in the TC, ITM+ATMC12.5, and ITM+ATMC25 groups compared to the HSC group.</div></div><div><h3>Conclusion</h3><div>The results indicate that adding 25 % more TM provided by ATMC than the recommended ITM levels can positively affect growth, intestinal structure, and stress-related physiological responses in broilers exposed to HS.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"91 ","pages":"Article 127708"},"PeriodicalIF":3.6,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144739624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the zinc-binding proteins in the mutational hotspots of human cancer","authors":"Shilpa Chauhan ,&nbsp;Mahesh Kulharia ,&nbsp;Shailender Kumar Verma","doi":"10.1016/j.jtemb.2025.127706","DOIUrl":"10.1016/j.jtemb.2025.127706","url":null,"abstract":"<div><div>Mutations and zinc (Zn) ions are crucial in cancer biology. This study examines the tripartite relationship between mutations, zinc-binding proteins (ZBPs), and cancer. A total of 75 ZBPs were identified that may contribute to cancer after undergoing mutations. It was determined that positively charged amino acids such as histidine, arginine, and lysine, alongside negatively charged aspartic acid and glutamic acid, as well as the polar amino acid cysteine, can bind to Zn ions in cancer-related ZBPs. Most cancer-related ZBPs are intracellularly localized, found in the nucleoplasm, nuclear vesicles, and nucleoli, and primarily function as transferases or DNA-binding proteins. These cancer-related ZBPs are located on chromosomes 3, 11, and 19. A total of 46 cancer-related ZBPs exhibited interactions with one another, demonstrating complex inter- and intracellular co-regulation of molecular functions and protein-protein interactions. Mutations impact the Zn ion binding sites of many cancer-related ZBPs. Eleven cancer-related ZBPs (TRIM24, WT1, BCL6, MECOM, PATZ1, IKZF3, ZBTB16, ZNF521, BCL11B, TRIM33, and RAF1) were identified as oncogenic, tumor suppressor, and fusion proteins. The analysis of mutations in the Zn ion binding sites of the ZBPs revealed that mutations affect the proteins’ structure, function, and binding affinity, potentially leading to cancer. Investigating mutations in the Zn binding sites of these proteins will pave the way for cancer treatments by enhancing our understanding of their role in cancer spread and invasion.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"91 ","pages":"Article 127706"},"PeriodicalIF":3.6,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144769479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oropharyngeal aspiration of particulate hexavalent chromium increases chromium levels in lung and liver, and induces essential metal dyshomeostasis in lung, liver, and blood","authors":"Idoia Meaza ,&nbsp;Jamie L. Wise ,&nbsp;Sandra S. Wise ,&nbsp;Haiyan Lu ,&nbsp;Aggie R. Williams ,&nbsp;Morgan Delnicki ,&nbsp;Jack Easley ,&nbsp;J. Calvin Kouokam ,&nbsp;John Pierce Wise Jr. ,&nbsp;Samuel T. Vielee ,&nbsp;James T.F. Wise ,&nbsp;Rachel M. Wise ,&nbsp;John Pierce Wise Sr.","doi":"10.1016/j.jtemb.2025.127705","DOIUrl":"10.1016/j.jtemb.2025.127705","url":null,"abstract":"<div><h3>Background</h3><div>Hexavalent chromium [Cr(VI)] is a known human lung carcinogen with widespread exposure risks, yet how it causes cancer is still unclear. Cr(VI) causes dyshomeostasis of essential metals in the brains of exposed rats and is considered a biomarker of lung cancer, yet whether Cr(VI) causes metal dyshomeostasis in the lung is unknown. Cr(VI) particles impact at lung bifurcations causing tumors at these sites, indicating the bronchial branching is a key consideration for its toxicity. This study chose guinea pigs because, like humans, they have a dichotomous bronchiole branching pattern. The aim of this study was to investigate Cr accumulation and essential metal dyshomeotasis in the lung, liver and blood.</div></div><div><h3>Methods</h3><div>We acutely and subchronically exposed guinea pigs to zinc chromate through oropharyngeal aspiration. We collected lung, liver and blood, and quantified metal levels using inductively coupled plasma-mass spectrometry.</div></div><div><h3>Results</h3><div>Our data indicate oropharyngeal Cr(VI) exposure results in Cr accumulation in the lung and liver. We also measured zinc levels, and despite using zinc chromate as our representative Cr(VI) compound we observed no changes. This study is the first study to consider Cr(VI)-induced essential metal dyshomeostasis in the lung, liver and blood, and we found Cr(VI) exposure changed the levels of essential metals like potassium, sodium, calcium, magnesium, iron, copper, cobalt, manganese, selenium and molybdenum.</div></div><div><h3>Conclusions</h3><div>This study established a new relevant model to study Cr(VI)-carcinogenesis and our results suggest metal dyshomeostasis could be part of the Cr(VI) carcinogenic mechanism and these effects should be considered in future mechanistic studies.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"91 ","pages":"Article 127705"},"PeriodicalIF":3.6,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on manganese and its effect on health and distribution in selected African countries","authors":"Irene Ebosereme Ainyanbhor ,&nbsp;Great Iruoghene Edo ,&nbsp;Patrick Othuke Akpoghelie ,&nbsp;Joseph Oghenewogaga Owheruo ,&nbsp;Tayser Sumer Gaaz ,&nbsp;Emad Yousif ,&nbsp;Endurance Fegor Isoje ,&nbsp;Ufuoma Augustina Igbuku ,&nbsp;Rapheal Ajiri Opiti ,&nbsp;Arthur Efeoghene Athan Essaghah ,&nbsp;Dina S. Ahmed ,&nbsp;Dilber Uzun Ozsahin ,&nbsp;Huzaifa Umar","doi":"10.1016/j.jtemb.2025.127707","DOIUrl":"10.1016/j.jtemb.2025.127707","url":null,"abstract":"<div><div>Manganese (Mn), an essential trace element, is important in various biochemical and physiological processes such as proper growth and development, digestion, immune response, energy metabolism, homeostasis, reproduction, and defense against oxidative stress through the enzyme manganese superoxide dismutase (MnSOD) in mitochondria of body cells. This review highlights the biological role and effects of manganese on human health as well as health condition associated with manganese. Furthermore, it discusses Mn distribution among people in Nigeria, Ghana and Egypt taking into cognizant the Mn status of food with a view of assessing their health status with respect to Mn. A daily intake of Mn that is insufficient is linked to a number of negative health effects, including reduced fertility, impaired bone formation, generalized growth impairment, and altered lipid, protein and carbohydrate metabolism. However, there are proposed strategy to mitigate Mn induced neurotoxicity. This review reveals there is insufficient data on Mn content of food consumed, drinking water and daily manganese intake of individuals including children in African countries like Nigeria, Ghana and Egypt. Available data reveals most of the popularly consumed food analyzed contains sufficient quantity of Mn and may provide the recommended daily intake (RDI) if the food items are adequately combined in their diet in these regions. Therefore, it is important to emphasize the need to evaluate the Mn content of food consumed, drinking water and daily intake of Mn in Nigeria, Ghana and Egypt.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"91 ","pages":"Article 127707"},"PeriodicalIF":3.6,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144724814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phospholipase C activator 3m3FBS reverses the synergistic toxicity of arsenic and cadmium","authors":"Manika Garg ,&nbsp;Aiysha Siddiq Khan ,&nbsp;Muskan Verma ,&nbsp;Anupam Chawla ,&nbsp;Pawan Yadav ,&nbsp;Yogita Rawat ,&nbsp;Asghar Ali ,&nbsp;Somendu Kumar Roy ,&nbsp;Mohan Kamthan","doi":"10.1016/j.jtemb.2025.127701","DOIUrl":"10.1016/j.jtemb.2025.127701","url":null,"abstract":"<div><h3>Background</h3><div>Arsenic (As), cadmium (Cd), and lead (Pb) frequently co-occur in environmental exposures and are among the top ten toxic substances of concern. While the individual toxicity of these metals is well established, their interactive effects remain poorly understood. This study investigates the toxicological interactions among As, Cd, and Pb, with a focus on calcium (Ca²⁺)-mediated signaling mechanisms.</div></div><div><h3>Methods</h3><div>We evaluated the cytotoxic and apoptotic effects of individual metals and their binary/trinary mixtures in <em>Saccharomyces cerevisiae</em>, N2a neuroblastoma cells, and primary hippocampal neurons. Calcium signaling involvement was probed using pharmacological modulators including calmodulin inhibitor (W-7), calcineurin inhibitor (CsA), and a phospholipase C activator (3m3FBS). Cell viability was assessed by MTT and spot assays; apoptosis was analyzed using Annexin V/PI flow cytometry and DAPI/PI staining.</div></div><div><h3>Results</h3><div>As and Cd exhibited synergistic toxicity, while Pb antagonized their effects in trinary mixtures. Pb-mediated rescue involved calmodulin and calcineurin pathways, suggesting Ca²⁺ mimicry. Direct CaCl₂ supplementation showed only modest effects. Importantly, 3m3FBS, a PLC activator, significantly reduced apoptosis in both N2a cells and primary neurons exposed to As+Cd, partially mimicking the protective effect of Pb.</div></div><div><h3>Conclusion</h3><div>Our findings highlight a Ca²⁺-dependent mechanism underlying the modulation of heavy metal toxicity. While Pb attenuates As+Cd-induced neurotoxicity via calcium signaling, its own toxicity limits therapeutic utility. Activation of PLC by 3m3FBS offers a promising alternative neuroprotective strategy against heavy metal-induced damage.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"91 ","pages":"Article 127701"},"PeriodicalIF":3.6,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144739625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human health risk of heavy metal biomagnification: Trophic transfer patterns in aquatic ecosystems","authors":"Saira Naz ,&nbsp;Syed Sikandar Habib ,&nbsp;Madeeha Arshad ,&nbsp;Saima Majeed ,&nbsp;Ümit Acar ,&nbsp;Osman Sabri Kesbiç ,&nbsp;Mohamed Mohany ,&nbsp;Francesca Aragona ,&nbsp;Francesco Fazio","doi":"10.1016/j.jtemb.2025.127704","DOIUrl":"10.1016/j.jtemb.2025.127704","url":null,"abstract":"<div><h3>Background</h3><div>Heavy metal contamination in aquatic ecosystems poses significant ecological and human health risks, particularly through trophic transfer in food webs.</div></div><div><h3>Objective</h3><div>This study investigates the mean concentrations and trophic transfer of heavy metals (Cd, Cu, Cr, and Pb) across various environmental compartments (water, sediment, plankton) and trophic levels (three fish species: <em>Catla. catla</em>, <em>Labeo rohita</em>, and <em>Cyprinus carpio</em>) in an aquatic ecosystem.</div></div><div><h3>Methodology</h3><div>Samples were collected in 2024 and heavy metals in the samples were determined using inductively coupled plasma atomic emission spectrometry (ICP-AES).</div></div><div><h3>Results</h3><div>Cu was most abundant in water (1.5–2.0 µg/L) and sediments (20–25 µg/g DW), while plankton accumulated high Cu and moderate Pb and Cr levels. Among fish, <em>C. carpio</em> showed the highest metal accumulation. Trophic magnification factor (TMF), which quantifies metal concentration trends across food chains, indicated biomagnification of Pb (TMF = 1.56) and Cd (TMF = 1.31), and biodilution of Cu (TMF = 0.64) and Cr (TMF = 0.73). Biomagnification factor (BMF), reflecting metal transfer from prey to predator, was highest for Pb in <em>C. carpio</em> (BMF = 3.89). Principal Component Analysis showed Cu and Pb enriched in sediments, while Cd was associated with plankton, indicating bioavailability at lower trophic levels. Although hazard index (HI) values were below the safety threshold for all fish species, <em>C. carpio</em> posed higher health risks due to elevated Cd and Pb levels.</div></div><div><h3>Conclusions</h3><div>Overall, the study reveals significant biomagnification of Pb and Cd, posing ecological and health risks, while Cu and Cr show biodilution. Mitigation requires integrated management, including source control, monitoring, ecological remediation, and public awareness.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"91 ","pages":"Article 127704"},"PeriodicalIF":3.6,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144721688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant-based protein supplements as emerging sources of metal exposure: A risk assessment study","authors":"Elena Bethencourt-Barbuzano ,&nbsp;Ewa Pyrzynska ,&nbsp;Katarzyna Siedzik ,&nbsp;Dailos González-Weller ,&nbsp;Soraya Paz-Montelongo ,&nbsp;Angel J. Gutiérrez ,&nbsp;Arturo Hardisson ,&nbsp;Malgorzata Misniakiewicz ,&nbsp;Montaña Cámara ,&nbsp;Carmen Rubio","doi":"10.1016/j.jtemb.2025.127703","DOIUrl":"10.1016/j.jtemb.2025.127703","url":null,"abstract":"<div><div>The growing demand for plant-based diets has prompted the food industry to develop nutritional supplements, particularly plant-based protein supplements (PS), designed to meet the needs of vegans and vegetarians. While these supplements offer essential nutrients, they may also contain potentially toxic elements (PTEs) that pose health risks. This study assessed the metal content of 56 plant-based PS samples from European sources using Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES). Dietary exposure to these elements was evaluated across three consumption scenarios, considering established reference intake values (AI/TDI/TWI/UL) and the Margin of Exposure (MOE) for lead (Pb). The analysis identified plant-based PS as significant sources of essential elements, including sodium (9101.66 mg/kg), potassium (5784.91 mg/kg), calcium (2201.45 mg/kg), molybdenum (1.63 mg/kg), manganese (25.14 mg/kg), copper (11.59 mg/kg), iron (132.58 mg/kg), zinc (62.56 mg/kg), chromium (0.45 mg/kg), and cobalt (0.11 mg/kg). However, these products also contained PTEs such as lead (0.07 mg/kg), cadmium (0.09 mg/kg), aluminum (18.39 mg/kg), nickel (1.19 mg/kg), strontium (5.67 mg/kg), barium (1.86 mg/kg), boron (4.59 mg/kg), and vanadium (0.05 mg/kg). At the recommended consumption level of 30 g/day, exposure to both essential and PTEs did not exceed reference intake limits. However, this dose accounted for 75 % of the Adequate Intake (AI) for molybdenum. Excessive consumption (100 g/day) raised concerns about exceeding the AI for copper (105.40 %) and magnesium (105.90 %) in women, as well as molybdenum (250.16 %) and iron (120.53 %) for men and postmenopausal women. These findings underscore the dual role of plant-based PS as valuable sources of essential nutrients and potential vectors for PTEs.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"91 ","pages":"Article 127703"},"PeriodicalIF":3.6,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The variability of selenium and sulphur and the impact of creatinine in blood and urine of humans","authors":"Dag G. Ellingsen ,&nbsp;Stephan Weinbruch ,&nbsp;Gerd Sallsten ,&nbsp;Balazs Berlinger ,&nbsp;Lars Barregard","doi":"10.1016/j.jtemb.2025.127700","DOIUrl":"10.1016/j.jtemb.2025.127700","url":null,"abstract":"<div><h3>Background</h3><div>Intake of selenium (Se) and sulphur (S) is mainly through meat and fish. This is also the case for creatine, which is broken down into creatinine (Crea).</div></div><div><h3>Objectives</h3><div>Objectives were to assess variability of Se and S in urine and blood, assess diurnal variation and associations with Crea in urine (U-Crea).</div></div><div><h3>Methods</h3><div>Urine was collected at six fixed times for 24 h, and repeated one week later among 29 males and 31 females. Blood was collected at both occasions.</div></div><div><h3>Results</h3><div>Mean 24 h urinary excretions of S (U-S_24 h) and Se (U-Se_24 h) on the first sampling day were 756 mg and 23 µg, respectively. U-S_24 h and U-Se_24 h were highly correlated (r = 0.75; p &lt; 0.001). Diurnal variation in the urinary excretion rate (ER) of S and Se was associated with urinary flow rate (UFR). Mean urinary concentrations of S and Se varied substantially during the day, except Crea adjusted concentrations. The 24 h U-Crea excretion was associated with U-S_24 h (r = 0.81; p &lt; 0.001) and U-Se_24 h (r = 0.60; p &lt; 0.001). The U-Crea ER were associated with those of S (r = 0.74; p &lt; 0.001) and Se (r = 0.83; p &lt; 0.001). The highest intraclass correlation coefficients (ICC) for Se was for the blood cellular fraction (0.898) and U-S_24 h (0.529) for S.</div></div><div><h3>Conclusions</h3><div>The urinary S and Se ER were highly associated with Crea ER, which has previously not been shown. This suggests that Crea corrected values should be interpreted with caution. Biomarkers of Se in blood had the highest ICC, while ICCs for S were generally lower. ICC of S and Se has rarely been studied. There was a slight diurnal variation in urinary ER of S and Se which was weakly associated with UFR.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"91 ","pages":"Article 127700"},"PeriodicalIF":3.6,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144703116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metal and metalloid contamination, oxidative stress and genotoxicity in Nile Tilapia (Oreochromis niloticus) from an important eutrophicated lagoon in southeastern Brazil","authors":"Julia Araujo Alves ,&nbsp;Tatiana Dillenburg Saint-Pierre ,&nbsp;Rachel Ann Hauser-Davis ,&nbsp;Fábio Veríssimo Correia ,&nbsp;André Luís de Sá Salomão ,&nbsp;Enrico Mendes Saggioro","doi":"10.1016/j.jtemb.2025.127697","DOIUrl":"10.1016/j.jtemb.2025.127697","url":null,"abstract":"<div><div>Coastal lagoons are highly susceptible to chemical contamination, due to their proximity to urban and industrial areas. This seasonal cross-sectional study based on primary data collection investigated the effects of metal and metalloid exposure on Nile tilapia (<em>Oreochromis niloticus</em>) from Jacarepaguá Lagoon (LJPA), a eutrophicated and urbanized coastal ecosystem in southeastern Brazil. Fish (n = 44) were sampled over one year, covering all four seasons at five fixed locations distributed across the lagoon, concurrently with surface water collection. Metal and metalloid concentrations in liver and muscle tissues were quantified by inductively coupled plasma mass spectrometry (ICP-MS). Analyzed biomarkers included MT, SOD, GST, GSH, LPO, and DNA damage (comet assay). Poor physicochemical and microbiological water quality according to Brazilian legislation were observed. Sampling during the spring and summer revealed greater metal diversity in tissues, likely influenced by increased rainfall and runoff. Arsenic, Cd, Hg and Pb were detected in both muscle and liver. Superoxide-dismutase activity decreased by 38–66.5 % in liver and 35–78 % in muscle, while GST activity in muscle decreased by 67.1–73.5 %. Muscle MT levels increased by 39–280 % across seasons, with peak liver MT values observed during spring (C4). Hepatic CARB increased by 119–662 %, especially in summer (C5), and the highest liver LPO levels were recorded in autumn (p &lt; 0.05). Significant correlations between metal concentrations and biomarkers included negative associations between Ti and LPO (ρ = –0.93) and MT (ρ = –0.85), and between Hg and GSH (ρ = –0.90). Muscle LPO was negatively associated with Ni (ρ = –0.72), while GSH showed positive correlations with Cd, Ni, Ti and Fe. DNA damage was significantly higher in individuals collected during the summer (C5), when several metals were detected, suggesting genotoxic effects likely driven by seasonal factors. A Principal Component Analysis revealed distinct seasonal patterns of both metal bioaccumulation and physiological stress, with greater separation among muscle samples and stronger associations with oxidative biomarkers in liver. These findings highlight the need for environmental monitoring and management strategies to mitigate the impacts of metal pollution on aquatic life in urban coastal lagoons.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"91 ","pages":"Article 127697"},"PeriodicalIF":3.6,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144704830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}