Effect of propolis supplementation on cadmium toxicity associated with renal and hepatic dysfunction in rats

IF 3.6 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Trace Elements in Medicine and Biology Pub Date : 2025-02-01 DOI:10.1016/j.jtemb.2024.127587

Huseyin Ayhan , Caglar Adiguzel , Keziban Korkmaz Bayram , Ali Tugrul Akin , Fatma Gokce Apaydin , Yusuf Kalender

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引用次数: 0

Abstract

Cadmium (Cd), one of the toxic heavy metals, is of great importance for public health due to its use in many industrial areas. Propolis is a natural product with antioxidant and anti-inflammatory properties collected from plants by honey bees. The aim of this study was to investigate the protective role of propolis against the potential toxic effects of cadmium chloride in blood, liver and kidney tissues. For this purpose, rats were divided into four groups. Control group, propolis (150 mg/kg b.w.) treated group, cadmium chloride (1.76 mg/kg b.w. 1/50 LD₅₀) treated group and cadmium chloride plus propolis (1.76 mg/kg b.w. and 150 mg/kg b.w. respectively) treated group. The substances were given to rats by gavage for 28 days. After 28 days of treatment, a statistically significant change was observed in serum biochemical parameters such as ALT, AST, BUN, LDH, urea, uric acid and creatinine, hematological parameters such as erythrocyte, hemoglobin and hematocrit, and IL-1β and IL-6 cytokine levels of cadmium chloride treated rats compared to the control group. In addition, significant changes were observed in mRNA expression levels of Casp-3, p53 and Tnf-α genes, HSP70, HSP90 and GRP78 protein levels and histopathological/immunohistochemical examinations. Improvement in biochemical and hematologic parameters, cytokines, mRNA expression, heat shock proteins and immunohistochemistry changes were observed in cadmium plus propolis treated group compared to cadmium treated group.

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蜂胶补充对大鼠肾和肝功能障碍相关镉中毒的影响。

镉（Cd）是一种有毒重金属，在许多工业领域被广泛使用，对公众健康具有重要意义。蜂胶是蜜蜂从植物中采集的具有抗氧化和抗炎特性的天然产物。本研究旨在探讨蜂胶对氯化镉在血液、肝脏和肾脏组织中的潜在毒性作用的保护作用。为此，将大鼠分为四组。对照组、蜂胶（150 mg/kg b.w）处理组、氯化镉（1.76 mg/kg b.w / 1/50 LD50）处理组和氯化镉加蜂胶（分别为1.76 mg/kg b.w和150 mg/kg b.w）处理组。给大鼠灌胃28 d。治疗28 d后，与对照组相比，氯化镉处理大鼠血清ALT、AST、BUN、LDH、尿素、尿酸、肌酐等生化指标，红细胞、血红蛋白、红细胞压积等血液学指标，以及IL-1β、IL-6细胞因子水平均有统计学意义。Casp-3、p53、Tnf-α基因mRNA表达水平、HSP70、HSP90、GRP78蛋白表达水平及组织病理学/免疫组化检查均发生显著变化。与镉处理组相比，镉加蜂胶处理组大鼠的生化、血液学指标、细胞因子、mRNA表达、热休克蛋白及免疫组织化学变化均有所改善。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Trace Elements in Medicine and Biology 医学-内分泌学与代谢

CiteScore

6.60

自引率

2.90%

发文量

202

审稿时长

85 days

期刊介绍： The journal provides the reader with a thorough description of theoretical and applied aspects of trace elements in medicine and biology and is devoted to the advancement of scientific knowledge about trace elements and trace element species. Trace elements play essential roles in the maintenance of physiological processes. During the last decades there has been a great deal of scientific investigation about the function and binding of trace elements. The Journal of Trace Elements in Medicine and Biology focuses on the description and dissemination of scientific results concerning the role of trace elements with respect to their mode of action in health and disease and nutritional importance. Progress in the knowledge of the biological role of trace elements depends, however, on advances in trace elements chemistry. Thus the Journal of Trace Elements in Medicine and Biology will include only those papers that base their results on proven analytical methods. Also, we only publish those articles in which the quality assurance regarding the execution of experiments and achievement of results is guaranteed.