Life Science Journal最新文献

{"title":"Estimasi Kandungan Biomassa dan Simpanan Karbon Hutan Mangrove, Mangunharjo, Semarang","authors":"Wahyu Ika Apriliana, Frida Purwanti, Nurul Latifah","doi":"10.15294/lifesci.v10i2.54447","DOIUrl":"https://doi.org/10.15294/lifesci.v10i2.54447","url":null,"abstract":"Mangrove forest is one of the coastal ecosystems that have a role for mitigating global warming in absorbing carbon from the atmosphere and storing it in the form of biomass. The aim of this research to know the content of biomass and carbon storage on the stems, carbon storage in sediments and estimation CO2 absorption of mangrove stems and sediment. This research was conducted in March 2021 in the Mangunharjo mangrove forest. The method used on this research is a survey method and using purposive samplimg to take samples in three research station by three plot transect. Analysis for the carbon storage in the stem using non-destructive method with allometric equations, while in the sediment using the LOI (Loss on Ignition) method. The result of biomass content and carbon storage in the mangrove stems 341,02 tons/ha and 156,87 tons/ha, carbon storage in the sediment 52,24 tons/ha and estimation CO2 absorption of mangrove stems and sediment based on carbon storage is 575,71 tons/ha, dan 191,73 tons/ha. Based on this research, the largest carbon storage and estimation CO2 absorption was on the stems of mangrove. \u0000  \u0000Hutan mangrove merupakan salah satu ekositem pesisir yang berperan dalam mitigasi pemanasan global dengan menyerap karbon dari atmosfer dan menyimpannya dalam bentuk biomassa. Tujuan penelitian ini adalah mengetahui kandungan biomassa dan simpanan karbon pada tegakan, nilai simpanan karbon pada sedimen serta estimasi kemampuan tegakan dan sedimen dalam menyerap CO2. Penelitian dilakukan pada bulan Maret 2021 di hutan mangrove, Mangunharjo. Metode yang digunakan dalam penelitian ini adalah metode survey dan metode yang digunakan untuk mengambil sampel adalah purposive sampling yang dilakukan di 3 stasiun penelitian. Analisis untuk menghitung simpanan karbon di tegakan mangrove menggunakan metode non destructive dengan persaaman allometrik, sedangkan di sedimen menggunakan metode LOI (Loss on Ignition).  Hasil nilai biomassa dan simpanan karbon pada tegakan mangrove yaitu 341,02 ton/ha, dan 156,87 ton/ha, simpanan karbon pada sedimen yaitu 52,24 ton/ha dan estimasi serapan CO2 pada tegakan dan sedimen berdasarkan simpanan karbon 575,71 ton/ha, dan 191,73 ton/ha. Berdasarkan hasil penelitian penyimpan karbon dan penyerap CO2 terbesar berada pada tegakan mangrove.","PeriodicalId":49909,"journal":{"name":"Life Science Journal","volume":"107 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75479276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efek Rebusan Sereh (Cymbopogon citratus) Terhadap Respon Stress Oksidatif Pada Tikus Wistar Jantan (Rattus norvegicus) Diabetes","authors":"Barinta Widaryanti, Nurul Khikmah, Nunung Sulistyani","doi":"10.15294/lifesci.v10i2.54457","DOIUrl":"https://doi.org/10.15294/lifesci.v10i2.54457","url":null,"abstract":"Diabetes mellitus is a metabolic disorder with hyperglycemia. The amount of diabetic patients in Indonesia will increase rapidly without suitable treatment.  High level of blood glucose will trigger oxidative stress. The objective of this research wa to investigate the effect of lemongrass on oxidative stress response that was indicated by serum and hepatic Total antioxidant capacity and Malondialdehyde level. Thirty male wistar rat were divided into five groups. K1 was a normal group, K2 was a normal group with lemongrass treatment, K3 was a diabetic group induced by a single dose of Streptozotocin at a concentration of 60 mg/kg body weight, K4 was a diabetic group that was treated with glibenclamid at a concentration of 0.09 mg/200 gr body weight, while K5 was a diabetic group treated with lemongrass at a concentration of 3.6 ml/200 gr body weight. The result showed that lemongrass was able to reduce blood glucose levels, serum MDA and hepatic MDA levels and increased the level of total antioxidant status \u0000  \u0000Diabetes adlah kelainan metabolisme yang ditandai dengan hiperglikemia. Jumlah penderita diabetes mellitus di Indonesia akan mengalami peningkatan jika tidak ada penanganan yang sesuai. Tinggi kadar glukosa darah dapat memicu terjadinya stress oksidatif. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian sereh terhadap respon stress oksidatif yang ditunjukan dengan kapasitas antioksidan total dan kadar malondialdehid. Tigapuluh ekor tikus wistar jantan dibagi menjadi lima kelompok. K1 adalah kelompok normal, K2 adalah kelompok normal dengan perlakuan sereh, K3 adalah kelompok diabetes yang diinduksi dengan streptozotocin 60 mg/kg berat badan single dose, K4 adalah kelompok diabetes yang diberi perlakuan dengan glibenklamid dengan dosis 0.09 mg/200 gr berat badan. Sedangkan K5 adalah kelompok diabetes yang dieri perlakuan dengan rebusan sereh dengan dosis 3.6 ml/200 gr berat badan. Hasil penelitian menunjukan bahwa rebusan sereh mampu menurunkan kadar glukosa darah, konsetrasi malondialdehid serum dan hepar, serta meningkatkan total antioksidan status pada serum dan hepar. ","PeriodicalId":49909,"journal":{"name":"Life Science Journal","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87376839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peranan Jenis Media, Sumber Hormon Alami dan Teknik Induksi Akar Planlet dalam Aklimatisasi Pule Pandak","authors":"S. Rochmah, E. S. Rahayu","doi":"10.15294/lifesci.v10i2.54445","DOIUrl":"https://doi.org/10.15294/lifesci.v10i2.54445","url":null,"abstract":"Pule pandak is a plant that has medicinal. Pule pandak seeds have a hard coat that makes it difficult to germinate naturally. Plant tissue culture can be an alternative that is able to overcome the difficulty of seed germination which then needs to be acclimatized. Factors that influence the success of acclimatization include media type, induction technique and growth regulators. This study aims to analyze the role of media types, natural hormone sources and plantlet root induction techniques. This study used a randomized block design with three factors, the type of media (cocopeat, vermiculite and sand), natural hormone sources (coconut water and bean sprouts extract) and root induction techniques (in vitro + ex vitro and ex vitro). The parameters observed were the number of roots, number of leaves, plant height and percentage of life. Data were analyzed by three-way Anava and Duncan's Multiple Range Test. The results showed that the type of media had no effect on the number of roots, number of leaves, plant height and percentage of life. Natural hormone sources of bean sprouts extract affect the number of roots, number of leaves and plant height. Root induction techniques in vitro + ex vitro affected number of roots. \u0000  \u0000Pule pandak adalah tanaman yang memiliki khasiat sebagai bahan obat. Biji pule pandak memiliki kulit biji yang keras sehingga sulit berkecambah secara alami. Kultur jaringan tanaman dapat menjadi salah satu alternatif yang mampu mengatasi kesulitan perkecambahan biji yang selanjutnya perlu dilakukan aklimatisasi. Faktor-faktor yang mempengaruhi keberhasilan aklimatisasi antara lain jenis media, teknik induksi dan zat pengatur tumbuh. Penelitian ini bertujuan untuk menganalisis peranan jenis media, sumber hormon alami dan teknik induksi akar planlet. Penelitian ini menggunakan rancangan acak kelompok tiga faktor yaitu jenis media (cocopeat, vermikulit dan pasir), sumber hormon alami (air kelapa dan ekstrak tauge) dan teknik induksi akar (in vitro+ex vitro dan ex vitro). Parameter yang diamati adalah jumlah akar, jumlah daun, tinggi tanaman dan persentase hidup. Data dianalisis dengan Anava tiga arah dan Duncan’s Multiple Range Test. Hasil penelitian menunjukkan bahwa jenis media tidak berpengaruh terhadap jumlah akar, jumlah daun, tinggi tanaman dan persentase hidup. Sumber hormon alami ekstrak tauge berpengaruh terhadap jumlah akar, jumlah daun dan tinggi tanaman. Teknik induksi akar secara in vitro+ex vitro berpengaruh terhadap jumlah akar.","PeriodicalId":49909,"journal":{"name":"Life Science Journal","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79942075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pengaruh Ekstrak Daun Suren (Toona sinensis Merr.) Pada Tanaman Cabai Rawit Yang Diinfeksi Spora Colletotrichum capsici Terhadap Pertumbuhan, Kandungan Pigmen Dan Vitamin C","authors":"Fitriatus Sadiah, Yulita Nurchayati, Endang Saptiningsih","doi":"10.15294/lifesci.v10i2.54442","DOIUrl":"https://doi.org/10.15294/lifesci.v10i2.54442","url":null,"abstract":"Anthracnose is a disease of cayenne pepper that can be caused by the fungus Colletotrichum capsici. Suren leaf extract contains antifungal compounds. This study aims to determine the potential of suren leaf extract as a fungicide and its effect on growth, content of chlorophyll, carotenoids and vitamin C in cayenne pepper plants. This study used a single factor completely randomized design (CRD) with 6 treatments consisting of 0%, 25%, 50%, 75%, 100% concentration of suren extract and mankozeb synthetic fungicide. Data were analyzed using One Way ANOVA and Duncan's Multiple Range Test (DMRT) further test. The results showed that leaf spot from anthracnose decreased from 50%-100% concentration. Plant height at 50% extract reached 140 cm. The number of leaves in 50% and 100% extracts were 182 and 184 leaves. The highest chlorophyll content in suren extract 75% is 43.20 µmol/g and smallest is at 100% which is 37 μmol/g. The highest carotenoid content in 100% suren extract was 8.64 µmol/g, while the smallest was at concentration of 75%, which was 4.68 µmol/g. This study concluded that suren leaf extract concentrations of 25%, 50%, 75%, and 100% respectively suppressed leaf spot by 8.06%; 32.25%; 41.93%; and 43.95%. Suren leaf extract can increase the growth of cayenne pepper, chlorophyll, carotenoids and vitamin C. \u0000  \u0000Antraknosa adalah penyakit pada tanaman cabai rawit yang dapat disebabkan jamur Colletotrichum capsici. Ekstrak daun suren mengandung senyawa-senyawa antifungi. Penelitian ini bertujuan mengetahui potensi ekstrak daun suren sebagai fungisida dan pengaruhnya terhadap pertumbuhan, kandungan klorofil, karotenoid dan vitamin C pada tanaman cabai rawit. Penelitian ini menggunakan rancangan acak lengkap (RAL) faktor tunggal dengan 6 perlakuan yang terdiri atas perlakuan ekstrak suren konsentrasi 0%, 25%, 50%, 75%, 100% dan perlakuan fungisida sintetik mankozeb. Data dianalisis menggunakan One Way ANOVA dan uji lanjut Duncan’s Multiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa bercak daun dari penyakit antraknosa menurun mulai dari konsentrasi 50%-100%. Tinggi tanaman pada ekstrak 50% mencapai 140 cm. Jumlah daun pada ekstrak 50% dan 100% adalah 182 dan 184 daun. Kandungan klorofil tertinggi pada ekstrak suren 75% yaitu 43,20 μmol/g dan terendah pada konsentrasi 100% yaitu sebesar 37 μmol/g. Kandungan karotenoid tertinggi pada ekstrak suren 100% yaitu 8,64 μmol/g, sedangkan terendah pada konsentrasi 75% yaitu 4,68 μmol/g. Penelitian ini disimpulkan bahwa ekstrak daun suren konsentrasi 25%, 50%, 75%, dan 100% berturut-turut menekan bercak daun sebesar 8,06%; 32,25%; 41,93%; dan 43,95%. Ekstrak daun suren dapat meningkatkan pertumbuhan cabai rawit, klorofil, karotenoid dan vitamin C.","PeriodicalId":49909,"journal":{"name":"Life Science Journal","volume":"532 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78340737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pengaruh Zat Pengatur Tumbuh 2,4-D dan Kinetin terhadap Pertumbuhan dan Morfologi Sel Gembili (Dioscorea esculenta)","authors":"Anisa Dewi Sri Puspitasari, Noor Aini Habibah","doi":"10.15294/lifesci.v10i2.54460","DOIUrl":"https://doi.org/10.15294/lifesci.v10i2.54460","url":null,"abstract":"Gembili (Dioscorea esculenta) bioactive compounds that can be used for treatment. Cell culture is a method that can be used to produce bioactive compounds from plant. The success of cell culture techniques is influenced by the type and concentration of plant growth  (PGR). This study aims to analyze the effect of 2,4-D and kinetin on the growth and morphology of gembili cells. Murashige & Skoog (MS) medium with the addition of 2,4-D and kinetin (1 ppm 2,4-D + 1 ppm kinetin; 1 ppm 2,4-D + 0,5 ppm kinetin; 0,5 ppm 2,4-D + 1 ppm kinetin; 0,5 ppm 2,4-D + 0,5 ppm kinetin; and 0 ppm 2,4-D + 0 ppm kinetin) were used for gembili cell growth. The observed parameters are fresh weight and morphology of cells. The results showed that the combination of 1 ppm 2,4-D + 1 ppm kinetin produces the highest fresh weight (2,1782 g). The resulting cells are predominantly globular, but there are some that differentiate into elongated ones. Cell color in all treatments was variated from light brown to darker brown. The findings may provide preliminary information using cell suspension cultures in the production of secondary metabolites from Dioscorea esculenta. \u0000  \u0000Gembili (Dioscorea esculenta) menghasilkan senyawa bioaktif yang dapat dimanfaatkan untuk pengobatan. Kultur sel merupakan metode yang dapat digunakan untuk produksi senyawa bioaktif yang terkandung dalam tumbuhan. Keberhasilan dari teknik kultur sel di antaranya dipengaruhi oleh jenis dan konsentrasi zat pengatur tumbuh (ZPT). Penelitian ini bertujuan untuk menganalisis pengaruh ZPT 2,4-D dan kinetin terhadap pertumbuhan dan morfologi sel gembili. Variabel bebas yang digunakan pada penelitian ini yaitu kombinasi antara ZPT 2,4-D dan kinetin (1 ppm 2,4-D + 1 ppm kinetin; 1 ppm 2,4-D + 0,5 ppm kinetin; 0,5 ppm 2,4-D + 1 ppm kinetin; 0,5 ppm 2,4-D + 0,5 ppm kinetin; dan 0 ppm 2,4-D + 0 ppm kinetin). Tipe kultur yang digunakan adalah kultur suspensi sel menggunakan media MS dengan penggojogan. Variabel terikat pada penelitian ini yaitu berat basah dan morfologi sel. Hasil penelitian menunjukkan bahwa kombinasi antara ZPT 1 ppm 2,4-D + 1 ppm kinetin menghasilkan berat basah yang paling tinggi yaitu sebesar 2,1782 gram. Sel yang dihasilkan dominan berbentuk globular, namun ada beberapa yang berdiferensiasi menjadi memanjang, sedangkan warna sel bervariasi dari cokelat muda hingga cokelat tua. Temuan dapat menjadi informasi awal menggunakan kultur suspensi sel dalam produksi senyawa metabolit sekunder dari gembili.","PeriodicalId":49909,"journal":{"name":"Life Science Journal","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82927870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Struktur Komunitas Makrozoobentos di Perairan Pesisir Kelurahan Mangunharjo sebagai Bioindikator Kualitas Perairan","authors":"Risna Gina Sofiyani, Max Rudolf Muskananfola, Bambang Sulardiono","doi":"10.15294/lifesci.v10i2.54446","DOIUrl":"https://doi.org/10.15294/lifesci.v10i2.54446","url":null,"abstract":"The coastal waters of Mangunharjo Village, Tugu, Semarang City are located in a densely populated residential area, and many activities of people around it. These activities can cause a decrease in water quality which will impact the biota. This study aims to determine the structure of the macrozoobenthos community, determine the level of pollution that occurs and determine the most affecting factors of macrozoobenthos community in the coastal waters of Mangunharjo Village. This research using the purposive sampling method. Result find that there were 22 species of makrozoobenthos. The diversity index value (H') is categorized as moderate to low with a mean value of H' = 0,79. The uniformity index value (E) indicates a depressed to stable condition with a mean value of E = 0,52. The dominance index value (C) indicates that there are species that dominates significantly. The similarity index value is dominant in the low category. The status of pollution in the coastal waters of Mangunharjo Village is classified as polluted water. The factors that most influence the abundance of macrozoobenthos in the coastal waters of Mangunharjo Village are water temperature, loam and clay sediment texture with a correlation value of 0,597; 0,474 and 0,453. \u0000  \u0000Perairan pesisir Kelurahan Mangunharjo, Kecamatan Tugu, Kota Semarang berada pada kawasan pemukiman padat penduduk yang banyak terdapat aktivitas warga di sekitarnya. Aktivitas – aktivitas tersebut dapat menyebabkan penurunan kualitas air yang akan berdampak pada biota.  Tujuan penelitian ini adalah untuk mengetahui struktur komunitas makrozoobentos, mengetahui tingkat pencemaran yang terjadi dan mengetahui faktor yang paling mempengaruhi komunitas makrozoobentos di perairan pesisir Kelurahan Mangunharjo. Penelitian ini menggunakan metode purposive sampling. Hasil penelitian menemukan 22 spesies makrozoobentos. Nilai Indeks Keanekaragaman (H’) dikategorikan sedang hingga rendah dengan nilai rerata H’ = 0,79. Nilai Indeks Keseragaman (E) mengindikasikan kondisi yang tertekan hingga stabil dengan nilai rerata E = 0,52. Nilai Indeks Dominansi (C) menunjukkan adanya jenis yang mendominansi secara signifikan. Nilai Indeks Kesamaan dominan berada dalam kategori yang rendah. Status pencemaran di perairan pesisir Kelurahan Mangunharjo tergolong tercemar. Faktor yang paling berpengaruh terhadap kelimpahan makrozoobentos di perairan pesisir Kelurahan Mangunharjo adalah suhu air, tekstur sedimen lempung dan tekstur sedimen liat dengan nilai korelasi 0,597; 0,474 dan 0,453.","PeriodicalId":49909,"journal":{"name":"Life Science Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88861856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Struktur Histopatologis Lambung Tikus Wistar yang Diberi Ekstrak Daun Singkong (Manihot utillisima Pohl.) secara Kronik","authors":"Nugrahaningsih Wh, Addina Nur Luthfiani, Lisdiana Lisdiana, Endah Peniati","doi":"10.15294/lifesci.v10i2.54459","DOIUrl":"https://doi.org/10.15294/lifesci.v10i2.54459","url":null,"abstract":"Cassava leaves contain sodium (Na), potassium (K), and iron (Fe) which can increase the body's blood pressure. In addition, the chemical content of cassava leaves can also act as an anti-hypertension agent. However, cassava leaves also contain hydrogen cyanide (HCN) which at certain doses can cause toxic effects. This study aims to determine the safety of long-term consumption by observing the damage to gastric organ cells in Wistar rats after administration of cassava leaf extract (Manihot uttilisima Pohl.) for 90 days. Cassava leaf extract was made by maceration method using distilled water as a solvent. This research is an experimental laboratory research with a posttest only control group research design. This study used 36 Wistar rats which were randomly divided into 4 groups consisting of group I (control) given 10 ml/w of distilled water, group II (treatment group) given cassava leaf extract 80 mg/bb, group III (treatment group) given cassava leaf extract 400 mg/bb, group IV (treatment group) was given cassava leaf extract 2000 mg/bb. Organ sampling was carried out after 90 days of treatment, all rats were killed by cervical dislocation, then their stomach organs were taken. To determine the histopathological structure between the control group and the treatment group, the data from the gastric histopathological test of Wistar rats were analyzed using descriptive analysis. did not affect the histopathological structure of the stomach of Wistar rats. \u0000  \u0000Daun singkong mengandung natrium (Na), kalium (K), dan besi (Fe) yang mampu meningkatkan tekanan darah tubuh.Selain itu, kandungan kimia dari daun singkong juga dapat berperan sebagai agen anti hipotensi. Akan tetapi, pada daun singkong juga mengandung hidrogen sianida (HCN) yang pada dosis tertentu dapat menyebabkan efek toksik. Penelitian ini bertujuan untuk menganalisis keamananan konsumsi jangka panjang dengan melihat kerusakan sel organ lambung pada tikus Wistar setelah pemberian ekstrak daun singkong (Manihot uttilisima Pohl.)  selama 90 hari. Ekstrak daun singkong dibuat dengan metode maserasi menggunakan pelarut akuades. Penelitian ini merupakan penelitian eksperimental laboratorik dengan rancangan penelitian postest only control group. Penelitian ini menggunakan 36 ekor tikus Wistar yang secara acak dibagi menjadi 4 kelompok terdiri dari kelompok I (kontrol) diberi akuades 10 ml/bb, kelompok II (kelompok perlakuan) diberi ekstrak daun singkong 80 mg/bb, kelompok III (kelompok perlakuan) diberi ekstrak daun singkong 400 mg/bb, kelompok IV (kelompok perlakuan) diberi ekstrak daun singkong  2000 mg/bb. Pengambilan sampel organ dilakukan setelah 90 hari perlakuan, semua tikus dimatikan denggan cara dislokasi cervicalis, kemudian organ lambungnya diambil. Untuk mengetahui struktur histopatologi antara kelompok kontrol dan kelompok perlakuan data hasil uji histopatologi lambung tikus Wistar dianalisis menggunakan analisis deskriptif.Hasil penelitian menunjukan pemberian ekstrak daun sing","PeriodicalId":49909,"journal":{"name":"Life Science Journal","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82099535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kesesuaian Vegetasi untuk Taman Kota Semarang","authors":"Nana Kariada Tri Martuti, Margareta Rahayuningsih, Khoirunisa Alfirdani","doi":"10.15294/lifesci.v10i2.54444","DOIUrl":"https://doi.org/10.15294/lifesci.v10i2.54444","url":null,"abstract":"Fullfilling of Green Open Spaces through the arrangement of urban parks. Vegetation is an important element of urban parks. The incompatibility of the vegetation can reduce the function of the urban parks. The aim of this research is to analyse the suitability of vegetation in urban parks in Semarang. This research used exploration method. The results of key performance index analysis, show that the suitability of vegetation in urban parks of Semarang ranges from 56.25% to 84.3%. Assessment of vegetation’s suitabilities is influenced by physical characteristics, such as canopy, roots, color of flower, and others. The functions of vegetation such as shade plants, absorbent pollutants, increasing aesthetic value, spacing and composition of vegetation also can affect the suitability of vegetation in urban parks. The vegetation’s suitabilities in urban parks in Semarang can be categorized as compatible, compatible enough, and not compatible. \u0000  \u0000Pemenuhan kebutuhan Ruang Terbuka Hijau (RTH) melalui penataan taman kota. Vegetasi merupakan elemen penting penyusun taman kota. Ketidaksesuaian vegetasi penyusun taman dapat menyebabkan berkurangnya fungsi taman kota. Penelitian ini bertujuan untuk menganalisis kesesuaian vegetasi yang ada di taman kota Semarang. Metode yang digunakan adalah eksplorasi. Hasil analisis key performance index menunjukkan kesesuaian vegetasi di taman kota Semarang berkisar antara 56,25% sampai dengan 84,3%. Penilaian terhadap kesesuaian vegetasi dipengaruhi oleh ciri fisik, seperti tajuk, perakaran, warna bunga dan lainnya. Fungsi dari tanaman seperti sebagai tanaman peneduh, penyerap polutan, penambah nilai estetika, arak tanam dan pengkomposisian tanaman dalam taman juga mempengaruhi kesesuaian vegetasi pada taman. Kesesuaian vegetasi di taman kota Semarang dapat dikategorikan sesuai, cukup sesuai dan kurang sesuai..","PeriodicalId":49909,"journal":{"name":"Life Science Journal","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78028713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolasi, Karakterisasi, dan Uji Aktivitas Antibakteri dari Bakteri Endofit Daun Kelor (Moringa oleifera)","authors":"Nadya Audina Nurkhafiya Sadikin, S. H. Bintari, T. Widiatningrum, Pramesti Dewi","doi":"10.15294/lifesci.v10i2.54441","DOIUrl":"https://doi.org/10.15294/lifesci.v10i2.54441","url":null,"abstract":"Endophytic bacteria are beneficial microorganisms that interact with host plants without causing any interference or damage to plants. Moringa oleifera is a medicinal plant that contains secondary metabolites and has antibacterial properties. The aim of this research is to isolate and characterize endophytic bacteria from Moringa oleifera leaves, and to test the antibacterial activity against pathogenic bacteria (Escherichia coli and Bacillus subtilus). The results of the isolation of Moringa leaf endophytic bacteria (Moringa oleifera) obtained amounted to one isolate. Based on the results of the catalase test, gram staining and spore observation, moringa oleifera leaf endophytic bacteria included in the genus Bacillus. The antibacterial test begins with the production of secondary metabolites of Moringa oleifera leaf endophytic bacteria, measures the rate of bacterial growth and tests the antibacterial activity using the Kirby Bauer method with paper disks. Observational data were analyzed statistically using ANOVA and further tests with Duncan (sig <0.05). Antibacterial activity test results showed that the incubation time of endophytic bacteria affected the growth of Escherichia coli and Bacillus subtilus bacteria. The incubation time of 10 hours showed the highest inhibition zone diameter of Escherichia coli and Bacillus subtilus bacteria respectively of 7.5 mm and 1.8 mm. The formation of inhibitory zones indicates the presence of secondary metabolite compounds from Moringa leaf endophytic bacteria which have an antibacterial effect. \u0000  \u0000Bakteri endofit merupakan mikroorganisme menguntungkan yang berinteraksi dengan tanaman inang tanpa menyebabkan gangguan atau kerusakan pada tanaman. Kelor (Moringa oleifera) merupakan tanaman obat yang mengandung metabolit sekunder dan berkhasiat sebagai antibakteri. Tujuan penelitian ini adalah mengisolasi dan mengkarakterisasi bakteri endofit dari daun kelor, serta menguji aktivitas antibakteri terhadap bakteri patogen (Escherichia coli dan Bacillus subtilus). Hasil isolasi bakteri endofit daun kelor (Moringa oleifera) yang diperoleh berjumlah satu isolat. Berdasarkan hasil uji katalase, pewarnaan gram dan pengamatan spora, bakteri endofit daun kelor termasuk ke dalam genus Bacillus. Uji antibakteri dimulai  dengan  produksi  metabolit  sekunder bakteri endofit daun kelor,  pengukuran  laju  pertumbuhan  bakteri  dan  uji  aktivitas  antibakteri menggunakan metode Kirby bauer dengan paper disk. Data hasil pengamatan dianalisis statistik menggunakan ANOVA dan uji lanjut dengan Duncan (sig < 0.05). Hasil uji aktivitas antibakteri menunjukkan bahwa waktu inkubasi bakteri endofit berpengaruh terhadap pertumbuhan bakteri Escherichia coli dan Bacillus subtilus. Waktu inkubasi selama 10 jam menunjukan diameter zona hambat tertinggi terhadap bakteri Escherichia coli dan Bacillus subtilus berturut-turut sebesar 7.5 mm dan 1.8 mm. Terbentuknya zona hambat mengindikasikan adanya senyawa metabolit sekunder dari bakte","PeriodicalId":49909,"journal":{"name":"Life Science Journal","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83335565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Combat Vehicle Operation on Cognitive Workload and Performance","authors":"Archana A. Malhari, D. Bhattacharyya, T. Chatterjee, K. Mani, M. Pal","doi":"10.14429/DLSJ.6.16818","DOIUrl":"https://doi.org/10.14429/DLSJ.6.16818","url":null,"abstract":"Multifaceted stress factors related to infantry combat vehicle (ICV) operation may be considered as major a source of cognitive workload, which may significantly impact the performance of infantry soldiers. The available literature on the effect of ICV’s operational environment on soldier’s cognitive workload is scanty or mostly unreported. The present study was designed to observe the effect of ICV operation on the i) cognitive workload ii) cognitive performance and iii) to study the association between cognitive workload and performance. Thirty soldiers [mean(SD)age: 31.86(2.9) years, weight: 74.40(7.7) kg, and height: 171.33(3.42) cm] volunteered for this study. Their heart rate (HR), heart rate variability (HRV) and respiratory frequency (RF) were recorded at three time points 00th-05th, 25th-30th and 55th-60th minute during the ICV operation. ‘A’ letter cancellation task (ALCT) was conducted to assess cognitive performance, before and after ICV operation. The internal temperature and relative humidity (RH) of ICV were assessed at same three time-points. Repeated measure ANOVA and Wilcoxon signed ranks test were conducted to observe significant changes in HR, HRV, RF, and cognitive performance. Association between HRV and ALCT was assessed using Pearson’s bivariate correlation. Changes were considered significant when p-value was ≤ 0.05. Significant increase in the HR and RF were observed along with significant decrease in both time and frequency domain of HRV after ICV operation. Similarly, ALCT showed a significant increase in the total and net score, and an increased error score in post-ICV operation. A strong positive correlation was observed between the ICV operation run-trial time and the increasing compartmental temperature (r=0.99) and RH (r=0.89). HRV components showed a negative correlation with ALCT measures. One hour of ICV operation resulted in increased cognitive workload and a significant decrease in the cognitive task performance. Internal temperature and RH of ICV are potential physical stress factors affecting the soldier’s workload and performance.","PeriodicalId":49909,"journal":{"name":"Life Science Journal","volume":"6 1","pages":"177-186"},"PeriodicalIF":0.0,"publicationDate":"2021-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49341871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}