{"title":"Peranan Jenis Media, Sumber Hormon Alami dan Teknik Induksi Akar Planlet dalam Aklimatisasi Pule Pandak","authors":"S. Rochmah, E. S. Rahayu","doi":"10.15294/lifesci.v10i2.54445","DOIUrl":null,"url":null,"abstract":"Pule pandak is a plant that has medicinal. Pule pandak seeds have a hard coat that makes it difficult to germinate naturally. Plant tissue culture can be an alternative that is able to overcome the difficulty of seed germination which then needs to be acclimatized. Factors that influence the success of acclimatization include media type, induction technique and growth regulators. This study aims to analyze the role of media types, natural hormone sources and plantlet root induction techniques. This study used a randomized block design with three factors, the type of media (cocopeat, vermiculite and sand), natural hormone sources (coconut water and bean sprouts extract) and root induction techniques (in vitro + ex vitro and ex vitro). The parameters observed were the number of roots, number of leaves, plant height and percentage of life. Data were analyzed by three-way Anava and Duncan's Multiple Range Test. The results showed that the type of media had no effect on the number of roots, number of leaves, plant height and percentage of life. Natural hormone sources of bean sprouts extract affect the number of roots, number of leaves and plant height. Root induction techniques in vitro + ex vitro affected number of roots. \n \nPule pandak adalah tanaman yang memiliki khasiat sebagai bahan obat. Biji pule pandak memiliki kulit biji yang keras sehingga sulit berkecambah secara alami. Kultur jaringan tanaman dapat menjadi salah satu alternatif yang mampu mengatasi kesulitan perkecambahan biji yang selanjutnya perlu dilakukan aklimatisasi. Faktor-faktor yang mempengaruhi keberhasilan aklimatisasi antara lain jenis media, teknik induksi dan zat pengatur tumbuh. Penelitian ini bertujuan untuk menganalisis peranan jenis media, sumber hormon alami dan teknik induksi akar planlet. Penelitian ini menggunakan rancangan acak kelompok tiga faktor yaitu jenis media (cocopeat, vermikulit dan pasir), sumber hormon alami (air kelapa dan ekstrak tauge) dan teknik induksi akar (in vitro+ex vitro dan ex vitro). Parameter yang diamati adalah jumlah akar, jumlah daun, tinggi tanaman dan persentase hidup. Data dianalisis dengan Anava tiga arah dan Duncan’s Multiple Range Test. Hasil penelitian menunjukkan bahwa jenis media tidak berpengaruh terhadap jumlah akar, jumlah daun, tinggi tanaman dan persentase hidup. Sumber hormon alami ekstrak tauge berpengaruh terhadap jumlah akar, jumlah daun dan tinggi tanaman. Teknik induksi akar secara in vitro+ex vitro berpengaruh terhadap jumlah akar.","PeriodicalId":49909,"journal":{"name":"Life Science Journal","volume":"50 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Life Science Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15294/lifesci.v10i2.54445","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
Pule pandak is a plant that has medicinal. Pule pandak seeds have a hard coat that makes it difficult to germinate naturally. Plant tissue culture can be an alternative that is able to overcome the difficulty of seed germination which then needs to be acclimatized. Factors that influence the success of acclimatization include media type, induction technique and growth regulators. This study aims to analyze the role of media types, natural hormone sources and plantlet root induction techniques. This study used a randomized block design with three factors, the type of media (cocopeat, vermiculite and sand), natural hormone sources (coconut water and bean sprouts extract) and root induction techniques (in vitro + ex vitro and ex vitro). The parameters observed were the number of roots, number of leaves, plant height and percentage of life. Data were analyzed by three-way Anava and Duncan's Multiple Range Test. The results showed that the type of media had no effect on the number of roots, number of leaves, plant height and percentage of life. Natural hormone sources of bean sprouts extract affect the number of roots, number of leaves and plant height. Root induction techniques in vitro + ex vitro affected number of roots.
Pule pandak adalah tanaman yang memiliki khasiat sebagai bahan obat. Biji pule pandak memiliki kulit biji yang keras sehingga sulit berkecambah secara alami. Kultur jaringan tanaman dapat menjadi salah satu alternatif yang mampu mengatasi kesulitan perkecambahan biji yang selanjutnya perlu dilakukan aklimatisasi. Faktor-faktor yang mempengaruhi keberhasilan aklimatisasi antara lain jenis media, teknik induksi dan zat pengatur tumbuh. Penelitian ini bertujuan untuk menganalisis peranan jenis media, sumber hormon alami dan teknik induksi akar planlet. Penelitian ini menggunakan rancangan acak kelompok tiga faktor yaitu jenis media (cocopeat, vermikulit dan pasir), sumber hormon alami (air kelapa dan ekstrak tauge) dan teknik induksi akar (in vitro+ex vitro dan ex vitro). Parameter yang diamati adalah jumlah akar, jumlah daun, tinggi tanaman dan persentase hidup. Data dianalisis dengan Anava tiga arah dan Duncan’s Multiple Range Test. Hasil penelitian menunjukkan bahwa jenis media tidak berpengaruh terhadap jumlah akar, jumlah daun, tinggi tanaman dan persentase hidup. Sumber hormon alami ekstrak tauge berpengaruh terhadap jumlah akar, jumlah daun dan tinggi tanaman. Teknik induksi akar secara in vitro+ex vitro berpengaruh terhadap jumlah akar.
普乐潘达克是一种具有药用价值的植物。紫色熊猫种子有一层坚硬的外壳,这使得它很难自然发芽。植物组织培养可以是一种替代方法,能够克服种子发芽的困难,然后需要适应。影响驯化成功的因素包括培养基类型、诱导技术和生长调节剂。本研究旨在分析培养基类型、天然激素来源和植株生根诱导技术的作用。本研究采用三因素随机区组设计,分别为培养基类型(椰泥、蛭石和沙子)、天然激素来源(椰子水和豆芽提取物)和根诱导技术(离体+离体和离体)。观察的参数为根数、叶数、株高和存活率。采用三向Anava和Duncan多元极差检验对数据进行分析。结果表明,培养基类型对根数、叶数、株高和成活率无显著影响。豆芽提取物的天然激素来源会影响根数、叶数和植株高度。离体根诱导+离体根诱导技术影响根数。Pule pandak adalah tanaman yang memoriliki khasiat sebagai bahan obat。Biji pule pandak memiliki kulit Biji yang keras sehinga sulit berkcambah secara alami。文化用语和tanaman dapat menjadi salah satu alternatif yang mampu mengatasi kesulitan perkecambahan biji yang selanjutnya perlu dilakukan aklimatisasi。杨Faktor-faktor mempengaruhi keberhasilan aklimatisasi安塔拉躺jenis媒体,teknik induksi丹扎- pengatur tumbuh。Penelitian ini bertujuan untuk menganalis, perananjenis media,数量荷尔蒙alami和teknikk induksi是一个行星。Penelitian ini menggunakan rancangan akak kelompok tiga因子为yaitjenis media (cocopeat, vermikulit dan pasir)、sumomonami (air kelapa dan ekstrak tauge)和teknik induksi akar(离体+离体)。参数yang diamati adalah jumlah akar, jumlah daun, tinggi tanaman dan表示hidup。数据分析采用Anava tiga arah dan Duncan的多重范围测试。Hasil penelitian menunjukkan bahwa jenis media tidak berpengaruh terhadap jumlah akar, jumlah dawan, tinggi tanaman和代表hidup。夏天,荷尔蒙的分泌量会增加,因为荷尔蒙的分泌量会增加,所以荷尔蒙的分泌量会减少。体外培养+离体培养。
期刊介绍:
Life Science Journal, the Acta Zhengzhou University Oversea Version, is an international journal with the purpose to enhance our natural and scientific knowledge dissemination in the world under the free opinion/idea publishing principle. The journal is calling for papers from all the world. All the valuable papers or reports that are related to life sciences - in their broadest sense - are welcome. Other academic articles that are only certain relevant but are of high quality will also be considered and published. Papers submitted could be reviews, objective descriptions, research reports, opinions/debates, news, letters, and other types of writings. All submitted manuscripts will be sent for external peer review and processed the editorial work. After the peer review, the journal will make the best efforts to publish the accepted valuable articles as soon as possible. Let''s work together to disseminate our research results and our opinions/ideas.