Life Science Journal最新文献

{"title":"Pertumbuhan Kecambah Kentang (Solanum tuberosum L.) secara In Vitro pada Konsentrasi NaClO dan Waktu Sterilisasi yang Berbeda","authors":"Khoirin Nida, Masrukhatul Luaeliyah, Yulita Nurchayati, Munifatul Izzati, Nintya Setiari","doi":"10.15294/lifesci.v10i1.47165","DOIUrl":"https://doi.org/10.15294/lifesci.v10i1.47165","url":null,"abstract":"Potato (Solanum tuberosum L.) have been cultivated with tubers. One alternative to the propagation of potatoes can be done with seeds that are germinated in vitro. The factors that influence the germination of potato seeds are the concentration of sterilant and time of sterilization. The purpose of this study was to determine the proper sterilant concentration and sterilization time to increase growth in the optimal percentage of germination and potato sprouts. The method used is in vitro culture with different concentrations and time treatment. This study used explants of potato seeds which were sterilized with a concentration of 15%  and 20%, 1 and 3 minutes of sterilization. The design used a completely randomized design 2x2 factorial. The factor are sterilant concentration and sterilization time. The parameters observed were germination time, plant height, number of leaves and roots. The results showed that sterile concentration did not affect of germination time but affected the germination. A 15% concentration indicates that germination occurs rapidly, while a 20% concentration inhibits germination. A 15% concentration results in an optimal number of leaves and plant height. The higher the concentration of sterile, tends to inhibit the growth of roots and leaves. \u0000Keywords: Sprouts; potatoes; in vitro culture; NaClO; growth; sterilization, Kecambah; kentang; kultur in vitro; NaClO; pertumbuhan; sterilisasi \u0000  \u0000Tanaman kentang (Solanum tuberosum L.) selama ini dibudidayakan dengan umbi. Salah satu alternatif perbanyakan kentang dapat dilakukan dengan biji yang dikecambahkan secara in vitro. Faktor yang mempengaruhi perkecambahan biji kentang adalah konsentrasi sterilan dan waktu sterilisasi. Tujuan penelitian ini adalah mengetahui konsentrasi sterilan (yang mengandung zat aktif 5,25% NaClO) dan waktu sterilisasi yang tepat untuk meningkatkan persentase perkecambahan dan pertumbuhan kecambah kentang. Metode yang digunakan adalah kultur in vitro biji kentang dengan perlakuan konsentrasi sterilan dan waktu sterilisasi yang berbeda. Penelitian ini menggunakan eksplan biji kentang yang dilakukan sterilisasi dengan konsentrasi sterilan 15% dan 20% dan waktu sterilisasi 1 menit dan 3 menit. Desain penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial 2x2. Faktor pertama adalah konsentrasi sterilan dan faktor kedua adalah waktu sterilisasi. Parameter yang diamati adalah waktu perkecambahan, tinggi tanaman, jumlah daun, dan jumlah akar. Hasil penelitian menunjukkan bahwa konsentrasi sterilan tidak berpengaruh terhadap waktu perkecambahan dan pertumbuhan kecambah. Konsentrasi sterilan 15% menyebabkan waktu perkecambahan cenderung lebih cepat dan konsentrasi sterilan 20% cenderung menghambat pertumbuhan kecambah. \u0000Keywords: Sprouts; potatoes; in vitro culture; NaClO; growth; sterilization, Kecambah; kentang; kultur in vitro; NaClO; pertumbuhan; sterilisasi","PeriodicalId":49909,"journal":{"name":"Life Science Journal","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73804654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pengendalian Rayap Tanah Coptotermes curvignathus Holmgren Menggunakan Ekstrak Daun Avicennia marina","authors":"Ulin Nikmatul Aflah, N. Subekti, R. S. R. Susanti","doi":"10.15294/lifesci.v10i1.47164","DOIUrl":"https://doi.org/10.15294/lifesci.v10i1.47164","url":null,"abstract":"Termite is one type of insect include order Isoptera, that recorded about 200 species in Indonesia and has been identified just 179 species. Economic losses aused of termites in Indonesia have reached billions rupiah each year. One of the control it using the vegetable insecticide, which can be used as an alternative to ontroled insect because it meets the criteria that is safe, cheap, and easy to apply the farmers. One of the ingredients that can be used as an insecticide substitute is the mangrove. Secondary metabolites found in mangroves   are   alkaloids,   phenolics,   steroids   and   terpenoids   where   the   active   component   is pharmacological, toxic and ecologic. This study aims to determine the effect of mangrove leaf extract of Avecennia marina as a vegetable pesticide against mortality of ground termites Coptotermes curvignathus Holmgren. The design of this study was experimental study with the dose tested 5 different treatments is0% (C-), 0.5%, 1%, 2% and borax 1% (C +) and the different duration is 3 hours, 5 hours, and 7 hours in5 repetitions. Data analysis using factorial test followed by Duncan test. Based on the result of the research, the concentration of 0,5% mangrove leaf extract solution with 3 hours long wood soak can kill the ground termite of Coptotermes curvignathus Holmgren 100% in 3 weeks. \u0000Keywords: termites, mangroves, pesticides vegetable rayap, bakau, pestisida   nabati \u0000  \u0000Rayap merupakan salah satu jenis serangga dalam ordo Isoptera yang tercatat di Indonesia sekitar 200 jenis dan baru 179 jenis yang sudah teridentifikasi. Kerugian ekonomis akibat rayap di Indonesia telah mencapai   milyaran   rupiah   tiap   tahunnya. Salah satu pengendaliannya dengan menggunakan insektisida nabati, yang dapat digunakan sebagai alternatif pengendalian serangga hama karena memenuhi kriteria yaitu aman, murah, dan mudah diterapkan petani. Salah satu bahan yang dapat digunakan sebagi bahan subtitusi insektisida adalah   bakau. Metabolit sekunder yang ditemukan pada bakau-bakauan adalah golongan alkaloid, fenolat, steroid dan terpenoid dimana komponen aktif tersebut bersifat farmakologik, toksik dan ekologik. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun bakau Avecennia marina   sebagai pestisida nabati terhadap mortalitas rayap tanah Coptotermes curvignathus Holmgren. Rancangan penelitian ini adalah penelitian eksperimen dengan dosis yang diujikan5 perlakuan berbeda yakni 0% (C-), 0,5%, 1%, 2% dan boraks 1% (C+) serta variasi lama rendam kayu 3 jam, 5 jam, dan 7 jam sebanyak 5 kali ulangan. Analisis data menggunakan uji Faktorial dilanjutkan uji Duncan. Berdasarkan hasil penelitian diperoleh konsentrasi larutan ekstrak daun bakau 0,5% dengan lama rendam kayu 3 jam efektif dapat membunuh rayap tanah Coptotermes curvignathus Holmgren sebesar 100% dalam 3 minggu. \u0000Keywords: termites, mangroves, pesticides vegetable,  rayap, bakau, pestisida   nabati","PeriodicalId":49909,"journal":{"name":"Life Science Journal","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78092455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pengembangan Taman Toga Sebagai Bahan Baku Resep Toga Berbasis Biosaince pada Kelompok Pkk Rw 7 Bangkong Kecamatan Gunungpati Kota Semarang","authors":"Retno Sri Iswari, R. S. R. Susanti, Sigit Saptono, Fitri Arum Sasi, Nafisatul Laila","doi":"10.15294/lifesci.v10i1.47176","DOIUrl":"https://doi.org/10.15294/lifesci.v10i1.47176","url":null,"abstract":"The spread of the COVID-19 virus that has occurred recently is fast the antiviral drugs are not yet available. The spread has to reduce the virus for mild sufferers, self-isolation is requested and for severe sufferers to be isolated in the hospital. One of the efforts to prevent the transmission of COVID-19 is to increase endurance through the use of family medicinal plants (Toga). The potential of medicinal plants as immunomodulators is widely spread in Bangkong village. Bangkong is one of the sub-districts in Semarang City that is also affected by Covid-19, each resident has enough yard to make Toga park. This service activity aims to: (1) provide skills to PKK RW 7 women in designing Toga gardens by making Toga plant racks to decorate their home gardens which can serve as the basic ingredients for making ethnoscience-based Toga recipes; (2) providing knowledge and skills in cultivating Toga; (3) providing knowledge and skills in making ethnoscience-based herbal recipes; (4) providing skills in compiling ethnoscience-based cookbooks about the benefits and ways of presenting them. The approach methods used in the implementation are workshops, lectures/outreach, and training / mentoring. The workshop, given to convey theoretical knowledge about landscape architecture based on land area. In this activity the community service team explained how to make Toga's garden suitable for the land, looking beautiful and free from being damaged by chickens. The results of the training were agreed by the PKK mothers to make Toga in the form of a shelf. It is hoped that the training at the first meeting will produce Toga garden designs that can ensure that each Toga plant can live freely without being disturbed by one another. The lecture method is carried out to explain the benefits of medicinal plants, types of medicinal plants, how to reproduce, and how to treat them. The training method is carried out to provide skills to mothers in making recipes and herbal medicine that can be used to prevent Covid-19 infection. Mothers are very responsive in participating in extension activities on various kinds of plants that can increase endur. After participating in this activity, mothers have understood well the benefits of Toga to increase endurance and prevent being infected with the Corona - 19 viruses \u0000Keywords: Bangkong Semarang, Eetnoscience, Taman toga, Bangkong Semarang, Eetnoscience, Taman toga \u0000  \u0000Penyebaran virus covid-19 yang terjadi akhir-akhir ini sangat cepat, obat antivirus belum tersedia. Untuk mengurangi penyebaran virus bagi penderita ringan diminta isolasi diri dan bagi penderita berat diisolasi di rumah sakit. Salah satu upaya pencegahan penularan covid-19 adalah dengan meningkatkan daya tahan tubuh melalui pemanfaatan tanaman obat keluarga (Toga). Potensi tanaman obat sebagai imunomodulator sangat banyak tersebar di lingkungan desa Bangkong. Bangkong merupakan salah satu kelurahan di Kota Semarang juga terdampak covid-19, tiap warga mampunyai ","PeriodicalId":49909,"journal":{"name":"Life Science Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89771960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perbedaan Kadar IgA dan IL6 Pada Penumpang “Biosmart and Safety Bus” pada Memakai Masker Herbal dan Masker Medis","authors":"Herera Rahajeng, Awal Prasetyo, N. Susilaningsih, Udadi Sadhana, Nani Maharani, Hairul Asri","doi":"10.15294/lifesci.v10i1.47177","DOIUrl":"https://doi.org/10.15294/lifesci.v10i1.47177","url":null,"abstract":"Viral infections that attack the respiratory tract are currently a major problem globally. The spread of viral infection can be one of the risks by passengers of the bus. The government's efforts being developed are the application of biosmart and safety buses designed to prevent the virus with stake holders. One of the health protocols is the use of masks. The purpose of this study was to see the differences in IgA and IL6 levels before and after the use of medical masks and herbal masks. Nasal wash samples were taken and analyzed by ELISA. The results obtained were the level of  IgA for the control group before wearing medical masks was 23.46834 pg/ml and after wearing 29.74473 pg/ml. While the IL6 levels before were 1.10544 ng / ml and after -2.04119 ng/ml. The results of the IgA in the experimental group before using the herbal mask 24.00840 pg/ml and after using was 29.74473 pg/ml. Meanwhile, the level of IL6 before was 0.91682 ng/ml and after 0.500157 ng/ml. Based on the results, there was an increase in IgA levels after the use of medical masks and herbal masks while IL6 levels decreased after the use of medical and herbal masks. \u0000Keywords: \u0000Herbal mask; IgA; IL6, Masker herbal;IgA;IL6 \u0000  \u0000Infeksi virus yang menyerang saluran pernapasan saat ini menjadi masalah utama secara global. Penyebaran agen infeksi virus dapat menjadi salah satu risiko yang dihadapi penumpang moda transportasi Bus. Upaya Pemerintah seperti yang saat ini dikembangkan adalah pengaplikasian biosmart and safety bus yang dirancang guna menghambat persebaran virus dengan stake holder yang tepat. Salah satu penerapan protokol kesehatan adalah penggunaan masker. Tujuan dari penelitian ini adalah melihat perbedaan kadar IgA dan IL6 sebelum dan sesudah pemakaian masker medis dan masker herbal pada penumpang biosmart and safety bus. Pengambilan sampel nasal wash dilakukan dan dianalisis dengan ELISA. Penelitian ini merupakan quasi experimental dengan prepost test randomized control design. Hasil yang didapat adalah kadar IgA untuk kelompok kontrol sebelum pemakaian masker medis 23,46834 pg/ml dan setelah memakai masker medis 29,74473 pg/ml. Sementara kadar IL6 sebelum adalah 1,10544 ng/ml dan setelah -2,04119 ng/ml. Hasil IgA kelompok eksperimen sebelum pemakaian masker herbal 24,00840 pg/ml dan setelah memakai masker herbal 29,74473 pg/ml. Sementara kadar IL6 sebelum adalah 0,91682 ng/ml dan setelah 0,500157 ng/ml. Berdasarkan hasil penelitian terdapat peningkatan kadar IgA setelah pemakaian masker medis dan masker herbal sementara kadar IL6 mengalami penurunan setelah pemakaian masker medis dan masker herbal. \u0000Keywords: \u0000Herbal mask; IgA; IL6, Masker herbal;IgA;IL6","PeriodicalId":49909,"journal":{"name":"Life Science Journal","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74741055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pengaruh Paparan Rokok Elektrik terhadap kadar GPx dan Catalase pada darah Tikus","authors":"M. Agustin, L. Lisdiana","doi":"10.15294/lifesci.v10i1.47174","DOIUrl":"https://doi.org/10.15294/lifesci.v10i1.47174","url":null,"abstract":"Electric cigarettes are cigarettes that are used with battery power, but do not burn tobacco like ordinary cigarette products. Electric cigarettes were initially said to be safe for health because nicotine solutions contained in e-cigarettes only consist of a mixture of water, propylene glycol, flavor enhancing substances, the aroma of tobacco, and other compounds that do not contain tar, tobacco or other toxic substances common in tobacco cigarettes. Electric cigarettes contain free radical compounds that can cause oxidative stress. In conditions of oxidative stress, free radical compounds increase lipid peroxide. In normal enzyme activity, an increase in the concentration of substrate, namely the molecule hydrogen peroxide (H2O2) can increase enzyme activity. But when the active side of all enzymes binds to the substrate, the addition of the substrate cannot increase the speed of the next enzyme reaction, resulting in a decrease in enzyme activity. This study aimed to analyze the levels of GPx and catalase in rat blood exposed to electrocution cigarettes. The study was conducted on 30 male white rats of Wistar strain which were divided into 5 groups, namely K- (negative control), K + (positive control), KP1 (treatment 1), KP2 (treatment 2) and KP3 (treatment 3). The K + group is a positive control group with 3 cigarettes and KP1, KP2, KP3 electric cigarette treatment groups treated with e-cigarettes with a dose of nicotine 3 mg, 6 mg, 9 mg for 30 days. To find out the difference in GPx and catalase levels, each group was analyzed by using one way annova and LSD for parametric test, while for non-parametric tests using the kruskal wallis test and Mann Whitney test. The results of the analysis showed that all between groups K-, K +, KP1, KP2 and KP3 were significantly different, in the KP3 group had the level of Gpx (40.25 ± 2.03e) and catalase (2.46 ± 9.50e) which was the lowest due to exposure electric cigarette with the highest dose of nicotine which is 9 mg. \u0000Keywords: Electric cigarettes, GPx, catalase, Rokok elektrik, GPx, katalase \u0000  \u0000Rokok elektrik adalah rokok yang digunakan dengan tenaga baterai, namun tidak membakar tembakau seperti produk rokok biasa. Rokok elektrik pada awalnya dikatakan aman bagi kesehatan karena larutan nikotin yang terdapat pada rokok elektrik hanya terdiri dari campuran air, propilen glikol, zat penambah rasa, aroma tembakau, serta senyawa-senyawa lain yang tidak mengandung tar, tembakau atau zat-zat toksik lain yang umum terdapat pada rokok tembakau. Rokok elektrik mengandung senyawa radikal bebas yang dapat menyebabkan stress oksidatif. Pada kondisi stress oksidatif, senyawa radikal bebas meningkatkan peroksida lipid. Dalam aktivitas enzim yang normal, peningkatan konsentrasi subtrat, yaitu molekul hidrogen peroksida (H2O2) dapat meningkatkan aktivitas enzim. Namun pada saat sisi aktif semua enzim berikatan dengan substrat, penambahan substrat tidak dapat meningkatkan kecepatan reaksi enzim selanjutnya, sehin","PeriodicalId":49909,"journal":{"name":"Life Science Journal","volume":"108 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74657057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pengaruh Pemberian Pakan dengan Penambahan Overripe Tempeh terhadap Konsumsi Pakan, Pertambahan Bobot Badan dan Konversi Pakan pada Ayam Petelur","authors":"Afra Fauziah L. Putri, S. H. Bintari","doi":"10.15294/lifesci.v10i1.47168","DOIUrl":"https://doi.org/10.15294/lifesci.v10i1.47168","url":null,"abstract":"Overripe tempeh is soybean tempeh which has undergone continuous fermentation. The use of overripe tempeh as a food ingredient in making traditional dishes was not done optimally. In fact, overripe tempeh contains isoflavones and probiotic compounds which are considered to improve the quality of animal feed. Isoflavone compounds contained in overripe tempeh have phytoestrogen properties in increasing the productivity of laying hens. This study aims to examine the effect of feeding with the addition of overripe tempeh with different concentrations on feed consumption, body weight gain, and feed conversion. This research was an experimental study with a completely randomized design (CRD) with 4 treatments and 7 replications. The research material used 28 layers of 94 week old laying hens. The feed treatment with the addition of overripe tempeh consisted of P0 (feed without overripe tempeh), P1 (7.5% addition of overripe tempeh), P2 (15% addition of overripe tempeh), and P3 (22.5% addition of overripe tempeh). The parameters observed consisted of feed consumption, body weight gain, and feed conversion. The data from the research results were analyzed for a variance with the F test at the 5% level. The results of the variance analysis showed that laying hens had a significant effect on feed consumption, but had no significant effect on body weight gain and feed conversion. It can be concluded that the use of tempeh overripe can be used up to 7.5% in layer hens feed. \u0000Keywords: overripe tempeh, layer chicken, performances, overripe tempeh, ayam petelur, performans. \u0000  \u0000Overripe tempeh merupakan tempe kedelai yang telah mengalami fermentasi yang berkelanjutan. Pemanfaatan overripe tempeh sebagai bahan makanan dalam penambahan masakan tradisional tidak dilakukan secara optimal. Padahal, overripe tempeh memiliki kandungan senyawa isoflavon dan kandungan probiotik yang dinilai dapat meningkatkan mutu pakan hewan ternak. Senyawa isoflavon yang terkandung didalamnya memiliki sifat fitoestrogen sehingga dapat meningkatkan produktivitas ayam petelur. Penelitian ini bertujuan untuk mengkaji pengaruh pemberian pakan dengan penambahan overripe tempeh dengan konsentrasi yang berbeda terhadap konsumsi pakan, pertambahan bobot badan dan konversi pakan. Penelitian yang dilakukan menggunakan rancangan acak lengkap (RAL) dengan 4 perlakuan dan 7 ulangan. Materi penelitian menggunakan ayam petelur umur 94 minggu sebanyak 28 ekor. Perlakuan pakan dengan penambahan overripe tempeh terdiri dari P0 (tanpa overripe tempeh), P1 (7,5% penambahan overripe tempeh), P2 (15% penambahan overripe tempeh), dan P3 (22,5% penambahan overripe tempeh). Parameter yang diamati terdiri dari konsumsi pakan, pertambahan bobot badan, dan konversi pakan. Data hasil penelitian dianalisis ragam dengan uji F pada taraf uji 5%, jika berpengaruh nyata dilanjutkan uji BNJ (5%). Hasil analisis ragam menunjukkan bahwa ayam petelur berpengaruh nyata terhadap konsumsi pakan, namun tidak berpengaruh nyat","PeriodicalId":49909,"journal":{"name":"Life Science Journal","volume":"146 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75093407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analisis Cemaran Coliform dan Identifikasi Escherichia coli dari Depo Air Minum Isi Ulang di Kota Semarang","authors":"Anita Citra Agustina","doi":"10.15294/lifesci.v10i1.47167","DOIUrl":"https://doi.org/10.15294/lifesci.v10i1.47167","url":null,"abstract":"The availability of drinking water that is suitable for consumption is currently starting to be limited, so the existence of a Refill Drinking Water Depot is an alternative. In principle, the water treatment process must be able to remove all types of pollutants, including Coliform bacteria and Esherichia coli bacteria which are indicators of pollution and unfavorable conditions for drinking water. Analysis of bacterial contamination in airways is one of the supporting factors in controlling water quality, especially on TV channels in Semarang City. This study aims to see the contamination of Coliform and Escherichia coli bacteria in raw water samples from the Refill Drinking Water Depot in Semarang City and to compare it with SNI quality standards to see the quality of drinking water feasibility. The results of this study indicate that out of 13 samples of raw water from the Refill Drinking Water Depot in Semarang City, 5 samples of which were positively contaminated with Coliform bacteria with MPN values, namely from Candisari District of 20 APM / 100 ml, Tembalang District 7 APM / 100 ml, District Ngaliyan 11 APM / 100 ml, West Semarang Subdistrict 4 APM / 100 ml, and Gunung Pati Subdistrict 4 APM / 100 ml and 2 samples were identified as positively contaminated with Esherichia coli bacteria on biochemical tests. The microbiological quality of the standard sample of refill drinking water from the Refill Drinking Water Depot in Semarang City is not suitable for consumption in accordance with SNI quality standards \u0000Keywords. Contamination, Coliform, Escherichia coli,  Refillable Drinking Water Depot, Kontaminasi, Coliform, Escherichia coli, Depot Air Minum Isi Ulang \u0000  \u0000Keberadaan air minum yang layak konsumsi saat ini mulai terbatas jumlahnya, sehingga keberadaan Depo Air Minum Isi Ulang menjadi salah satu alternatif. Proses pengolahan air pada prinsipnya harus mampu menghilangkan semua jenis polutan, termasuk bakteri Coliform dan bakteri Esherichia coli yang merupakan indikator adanya polusi kotoran dan kondisi yang tidak baik terhadap air minum. Analisis cemaran bakteri pada air minum menjadi salah faktor pembantu dalam mengontrol kualitas air minum khususnya yang beredar di Kota Semarang. Penelitian ini bertujuan untuk mengetahui cemaran bakteri Coliform dan Escherichia coli  pada sampel air baku dari Depo Air Minum Isi Ulang di Kota Semarang serta membandingkannya dengan standar baku mutu SNI untuk mengetahui kualitas kelayakan air minum. Hasil penelitian ini menunjukkan bahwa dari 13 sampel air baku dari Depo Air Minum Isi Ulang di Kota Semarang 5 sampel diantaranya positif tercemar bakteri Coliform dengan nilai MPN yaitu dari Kecamatan Candisari sebesar 20 APM/100 ml, Kecamatan Tembalang 7 APM/100 ml, Kecamatan Ngaliyan 11 APM/100 ml, Kecamatan Semarang Barat 4 APM/100 ml, dan Kecamatan Gunung Pati 4 APM/100 ml serta 2 sampel teridentifikasi positif tercemar bakteri Esherichia coli pada uji biokimia. Kualitas mikrobiologis sampel baku air","PeriodicalId":49909,"journal":{"name":"Life Science Journal","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80576557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pengaruh Penambahan Bioaktivator Terhadap Peningkatan Unsur Hara Pupuk Kandang Dan Aplikasinya Pada Pertumbuhan Tanaman Salak Pascaerupsi Merapi","authors":"Chusnul Adib Achmad","doi":"10.15294/lifesci.v10i1.47175","DOIUrl":"https://doi.org/10.15294/lifesci.v10i1.47175","url":null,"abstract":"The volcanic ash resulting from the eruption of Merapi Mount entered the pore space of the soil and affected the growth of salak plants. Post-eruption repair and recovery efforts must be carried out immediately through the use of manure with the addition of bio activators. The purpose of this study is to analyze the effect of adding different bio activators on the increase in the nutrient content of manure and zalacca plant growth and to determine the types of bio activators that can increase nutrient content and growth of salak pondok plants. This research was conducted using a completely randomized design (CRD). With a single factor, namely the difference in manure bio activators consisting of three levels, namely: manure with bio activator EM4, manure with bio activator Orgadec, and manure without bio activator. The results showed that manure with bio activator EM4 produced the highest nutrient content, including nitrogen at 1.14%, phosphate by 4.20%, and potassium at 0.74%. Manure with the addition of bio activator EM4 resulted in the lowest C / N ratio value of 8.33 and a pH value of 7.70. Measurement of plant height growth research parameters showed significantly different results. The results of measurements on the plant dry weight were not significantly different in the control and bio activator EM treatments \u0000Keywords: Keyword : manure nutrient, soil nutrient, bioactivator, hara pupuk kandang, hara tanah, bioaktivator \u0000  \u0000Debu vulkanik akibat erupsi Gunung Merapi masuk ke dalam pori-pori tanah dan mempengaruhi pertumbuhan tanaman salak. Upaya perbaikan dan pemulihan pasca erupsi harus segera dilakukan melalui penggunaan pupuk kandang dengan penambahan bio activator. Tujuan penelitian ini adalah untuk menganalisis pengaruh penambahan berbagai bio aktivator terhadap peningkatan kandungan hara pupuk kandang dan pertumbuhan tanaman salak serta untuk mengetahui jenis bio aktivator yang dapat meningkatkan kandungan hara dan pertumbuhan tanaman salak pondok. Penelitian ini dilakukan dengan menggunakan Rancangan Acak Lengkap (RAL). Dengan faktor tunggal yaitu perbedaan bio activator pupuk kandang yang terdiri dari tiga taraf yaitu: pupuk kandang dengan bio activator EM4, pupuk kandang dengan bio activator Orgadec, dan pupuk kandang tanpa bio activator. Hasil penelitian menunjukkan bahwa pupuk kandang dengan bio activator EM4 menghasilkan kandungan hara tertinggi, meliputi nitrogen 1,14%, fosfat 4,20%, dan kalium 0,74%. Pupuk kandang dengan penambahan bio aktivator EM4 menghasilkan nilai rasio C / N terendah sebesar 8,33 dan nilai pH 7,70. Pengukuran parameter penelitian pertumbuhan tinggi tanaman menunjukkan hasil yang berbeda nyata. Hasil pengukuran bobot kering tanaman tidak berbeda nyata pada perlakuan kontrol dan bio aktivator EM \u0000Keywords: Keyword : manure nutrient, soil nutrient, bioactivator, hara pupuk kandang, hara tanah, bioaktivator","PeriodicalId":49909,"journal":{"name":"Life Science Journal","volume":"170 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79365894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Keanekaragaman Jenis Burung di Kawasan Taman Wisata Alam Hutan Gambut Baning Sintang","authors":"Harits Yowansyah YPandayu Putra, A. Yanti, Riyandi Riyandi","doi":"10.15294/lifesci.v10i1.47172","DOIUrl":"https://doi.org/10.15294/lifesci.v10i1.47172","url":null,"abstract":"Research about bird species diversity in Nature Park of Peat Forest Baning Sintang was conducted in December 2018 to February 2019. This study aims to obtain information about bird species diversity in four types area Nature Park of Peat Forest Baning Sintang and influence of vegetation, noise levels and human activities for bird species diversity. Data collection is done by determining the point and time of observation, observation of bird diversity, vegetation, measuring environment parameters, noise levels and human activities. The results of data collection were analyzed to description analysis and PCA. The results showed 32 species of birds and 989 birds were found. The highest diversity index is the primary forest type area (H '= 2.51) and the lowest diversity index is the residential type area (H' = 0.62). Based on PCA analysis, humidity is the most influential environmental factor in primary forest, while noise is the most influential environmental factor in residential. \u0000Keywords: diversity; bird; Baning Sintang Forest, keanekaragaman; burung; Hutan Baning Sintang \u0000  \u0000Penelitian tentang Keanekaragaman Jenis Burung di Kawasan Taman Wisata Alam Hutan Gambut Baning Sintang telah dilakukan pada bulan Desember 2018 sampai Februari 2019. Penelitian ini bertujuan untuk mengetahui keanekaragaman jenis burung di empat tipe kawasan TWA Hutan Gambut Baning Sintang dan  pengaruh vegetasi, tingkat kebisingan dan aktivitas manusia terhadap keanekaragaman jenis burung. Pengumpulan data dilakukan dengan cara menentukan titik dan waktu pengamatan, pengambilan data keanekaragaman dan kekayaan burung, vegetasi, mengukur parameter lingkungan, tingkat kebisingan dan aktivitas manusia. Hasil pengumpulan data dianalisis dan dihubungkan dengan analisis deskripsi dan PCA. Hasil yang diperoleh menunjukkan 32 jenis burung dan 989 ekor burung yang ditemukan. Indeks keanekaragaman tertinggi berada di tipe penutupan lahan hutan primer (H’ = 2,51) dan indeks keanekaragaman terendah berada di tipe penutupan lahan pemukiman (H’ = 0,62). Berdasarkan analisis PCA, kelembaban adalah faktor lingkungan yang paling berpengaruh di hutan primer, sedangkan suara adalah faktor lingkungan yang paling berpengaruh di pemukiman. \u0000Keywords: diversity; bird; Baning Sintang Forest, keanekaragaman; burung; Hutan Baning Sintang","PeriodicalId":49909,"journal":{"name":"Life Science Journal","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80011528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protests Mass Movements and Politicised Identities A Relook at Social Conflicts in Contemporary India","authors":"Swati Mukherjee","doi":"10.14429/DLSJ.6.16637","DOIUrl":"https://doi.org/10.14429/DLSJ.6.16637","url":null,"abstract":"In recent years India has been witnessing an upsurge of conflicts over myriad issues such as displacement related to development projects, legislative changes, claim over natural resources, environmental issues and the like. Though each conflict situation is unique and demands a detailed research exploration in its own right, the need for evolving a paradigm based on underlying commonalities and basic principles cannot be denied. Such a framework would facilitate not only the researchers, but also the policymakers, and has the potential to create pathways for conflict containment and resolution. Even a cursory perusal of the underlying dynamics of most conflicts at local, national or international levels indicates a contestation over limited resources that eventually transforms into contestations for power and identity. Many such conflicts often appear to undermine national security and are often construed as ‘anti-development’, ‘anti-establishment’ or even ‘anti-state’. The present paper posits that it is important to reveal the subtler processes of negotiation in a conflict situation, to understand conflict as a co-constructed social reality and to highlight the reciprocal impact made by the dynamics of such constructions on identities of the parties involved. Along with examining the role of identity dynamics in perpetuating conflicts, the paper argues for incorporating both identity and instrumental pathways in theorizing conflicts. Emphasising upon the role played by politicisation of identities in conflict situations, the paper proposes a theoretical model for deconstructing conflicts and working towards conflict resolution.","PeriodicalId":49909,"journal":{"name":"Life Science Journal","volume":"6 1","pages":"5-10"},"PeriodicalIF":0.0,"publicationDate":"2021-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45202811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}