Microbiology-Sgm最新文献_第4页

'Wild Type'. 野生型"。

IF 2.6 4区生物学

Microbiology-Sgm Pub Date : 2024-08-01 DOI: 10.1099/mic.0.001495

Isabel Askenasy, Jemima E V Swain, Pok-Man Ho, Rahan Rudland Nazeer, Amelie Welch, Éva Bernadett Bényei, Leonardo Mancini, Sivan Nir, Pinyu Liao, Martin Welch

引用次数: 0

Mobility and growth in confined spaces are important mechanisms for the establishment of Bacillus subtilis in the rhizosphere. 在密闭空间中的移动和生长是枯草芽孢杆菌在根瘤层中建立的重要机制。

IF 2.6 4区生物学

Microbiology-Sgm Pub Date : 2024-08-01 DOI: 10.1099/mic.0.001477

Ilonka C Engelhardt, Nicola Holden, Tim J Daniell, Lionel X Dupuy

{"title":"Mobility and growth in confined spaces are important mechanisms for the establishment of Bacillus subtilis in the rhizosphere.","authors":"Ilonka C Engelhardt, Nicola Holden, Tim J Daniell, Lionel X Dupuy","doi":"10.1099/mic.0.001477","DOIUrl":"10.1099/mic.0.001477","url":null,"abstract":"The rhizosphere hosts complex and abundant microbiomes whose structure and composition are now well described by metagenomic studies. However, the dynamic mechanisms that enable micro-organisms to establish along a growing plant root are poorly characterized. Here, we studied how a motile bacterium utilizes the microhabitats created by soil pore space to establish in the proximity of plant roots. We have established a model system consisting of Bacillus subtilis and lettuce seedlings co-inoculated in transparent soil microcosms. We carried out live imaging experiments and developed image analysis pipelines to quantify the abundance of the bacterium as a function of time and position in the pore space. Results showed that the establishment of the bacterium in the rhizosphere follows a precise sequence of events where small islands of mobile bacteria were first seen forming near the root tip within the first 12-24 h of inoculation. Biofilm was then seen forming on the root epidermis at distances of about 700-1000 µm from the tip. Bacteria accumulated predominantly in confined pore spaces within 200 µm from the root or the surface of a particle. Using probabilistic models, we could map the complete sequence of events and propose a conceptual model of bacterial establishment in the pore space. This study therefore advances our understanding of the respective role of growth and mobility in the efficient colonization of bacteria in the rhizosphere.","PeriodicalId":49819,"journal":{"name":"Microbiology-Sgm","volume":"170 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11574552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141898751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of gene targets that potentiate the action of rifampicin on Mycobacterium bovis BCG. 鉴定可增强利福平对卡介苗分枝杆菌作用的基因靶标。

IF 2.6 4区生物学

Microbiology-Sgm Pub Date : 2024-08-01 DOI: 10.1099/mic.0.001488

Pooja Chand, Tom A Mendum, Rachel E Butler, Suzanne M Hingley-Wilson, Graham R Stewart

{"title":"Identification of gene targets that potentiate the action of rifampicin on Mycobacterium bovis BCG.","authors":"Pooja Chand, Tom A Mendum, Rachel E Butler, Suzanne M Hingley-Wilson, Graham R Stewart","doi":"10.1099/mic.0.001488","DOIUrl":"10.1099/mic.0.001488","url":null,"abstract":"Tuberculosis (TB) caused by bacteria of the Mycobacterium tuberculosis complex remains one of the most important infectious diseases of mankind. Rifampicin is a first line drug used in multi-drug treatment of TB, however, the necessary duration of treatment with these drugs is long and development of resistance is an increasing impediment to treatment programmes. As a result, there is a requirement for research and development of new TB drugs, which can form the basis of new drug combinations, either due to their own anti-mycobacterial activity or by augmenting the activity of existing drugs such as rifampicin. This study describes a TnSeq analysis to identify mutants with enhanced sensitivity to sub-minimum inhibitory concentrations (MIC) of rifampicin. The rifampicin-sensitive mutants were disrupted in genes of a variety of functions and the majority fitted into three thematic groups: firstly, genes that were involved in DNA/RNA metabolism, secondly, genes involved in sensing and regulating mycobacterial cellular systems, and thirdly, genes involved in the synthesis and maintenance of the cell wall. Selection at two concentrations of rifampicin (1/250 and 1/62 MIC) demonstrated a dose response for mutants with statistically significant sensitivity to rifampicin. The dataset reveals mechanisms of how mycobacteria are innately tolerant to and initiate an adaptive response to rifampicin; providing putative targets for the development of adjunctive therapies that potentiate the action of rifampicin.","PeriodicalId":49819,"journal":{"name":"Microbiology-Sgm","volume":"170 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11329110/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141989376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergy between Group 2 capsules and lipopolysaccharide underpins serum resistance in extra-intestinal pathogenic Escherichia coli. 第 2 组胶囊和脂多糖之间的协同作用是肠道外致病性大肠杆菌血清抗性的基础。

IF 2.6 4区生物学

Microbiology-Sgm Pub Date : 2024-08-01 DOI: 10.1099/mic.0.001493

Naoise McGarry, Domhnall Roe, Stephen G J Smith

{"title":"Synergy between Group 2 capsules and lipopolysaccharide underpins serum resistance in extra-intestinal pathogenic Escherichia coli.","authors":"Naoise McGarry, Domhnall Roe, Stephen G J Smith","doi":"10.1099/mic.0.001493","DOIUrl":"10.1099/mic.0.001493","url":null,"abstract":"Escherichia coli (E. coli) is a major cause of urinary tract infections, bacteraemia, and sepsis. CFT073 is a prototypic, urosepsis isolate of sequence type (ST) 73. This laboratory, among others, has shown that strain CFT073 is resistant to serum, with capsule and other extracellular polysaccharides imparting resistance. The interplay of such polysaccharides remains under-explored. This study has shown that CFT073 mutants deficient in lipopolysaccharide (LPS) O-antigen and capsule display exquisite serum sensitivity. Additionally, O-antigen and LPS outer core mutants displayed significantly decreased surface K2 capsule, coupled with increased unbound K2 capsule being detected in the supernatant. The R1 core and O6 antigen are involved in the tethering of K2 capsule to the CFT073 cell surface, highlighting the importance of the R1 core in serum resistance. The dependence of capsule on LPS was shown to be post-transcriptional and related to changes in cell surface hydrophobicity. Furthermore, immunofluorescence microscopy suggested that the surface pattern of capsule is altered in such LPS core mutants, which display a punctate capsule pattern. Finally, targeting LPS biosynthesis using sub-inhibitory concentrations of a WaaG inhibitor resulted in increased serum sensitivity and decreased capsule in CFT073. Interestingly, the dependency of capsule on LPS has been observed previously in other Enterobacteria, indicating that the synergy between these polysaccharides is not just strain, serotype or species-specific but may be conserved across several pathogenic Gram-negative species. Therefore, using WaaG inhibitor derivatives to target LPS is a promising therapeutic strategy to reduce morbidity and mortality by reducing or eliminating surface capsule.","PeriodicalId":49819,"journal":{"name":"Microbiology-Sgm","volume":"170 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11342863/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142037539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A tradeoff between bacteriophage resistance and bacterial motility is mediated by the Rcs phosphorelay in Escherichia coli. 大肠杆菌中的 Rcs 磷酸化链介导了噬菌体抗性与细菌运动性之间的权衡。

IF 2.6 4区生物学

Microbiology-Sgm Pub Date : 2024-08-01 DOI: 10.1099/mic.0.001491

Alita R Burmeister, Harleen Tewatia, Chloé Skinner

{"title":"A tradeoff between bacteriophage resistance and bacterial motility is mediated by the Rcs phosphorelay in Escherichia coli.","authors":"Alita R Burmeister, Harleen Tewatia, Chloé Skinner","doi":"10.1099/mic.0.001491","DOIUrl":"10.1099/mic.0.001491","url":null,"abstract":"Across the tree of life, pleiotropy is thought to constrain adaptation through evolutionary tradeoffs. However, few examples of pleiotropy exist that are well explained at the genetic level, especially for pleiotropy that is mediated by multiple genes. Here, we describe a set of pleiotropic mutations that mediate two key fitness components in bacteria: parasite resistance and motility. We subjected Escherichia coli to strong selection by phage U136B to obtain 27 independent mucoid mutants. Mucoidy is a phenotype that results from excess exopolysaccharide and can act as a barrier against viral infection but can also interfere with other cellular functions. We quantified the mutants' phage resistance using efficiency of plaquing assays and swimming motility using swim agar plates, and we sequenced the complete genomes of all mutants to identify mucoid-causing mutations. Increased phage resistance co-occurred with decreased motility. This relationship was mediated by highly parallel (27/27) mutations to the Rcs phosphorelay pathway, which senses membrane stress to regulate exopolysaccharide production. Together, these results provide an empirical example of a pleiotropic relationship between two traits with intermediate genetic complexity.","PeriodicalId":49819,"journal":{"name":"Microbiology-Sgm","volume":"170 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541549/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142082381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural products from food sources can alter the spread of antimicrobial resistance plasmids in Enterobacterales. 食物来源的天然产品可改变肠杆菌抗菌性质粒的传播。

IF 2.6 4区生物学

Microbiology-Sgm Pub Date : 2024-08-01 DOI: 10.1099/mic.0.001496

Ilyas Alav, Parisa Pordelkhaki, Judith Rodriguez-Navarro, Onalenna Neo, Celia Kessler, Ruth Jesujobalayemi Awodipe, Poppy Cliffe, Nivethanaa Pulavan, Huba L Marton, Simon Gibbons, Michelle M C Buckner

{"title":"Natural products from food sources can alter the spread of antimicrobial resistance plasmids in Enterobacterales.","authors":"Ilyas Alav, Parisa Pordelkhaki, Judith Rodriguez-Navarro, Onalenna Neo, Celia Kessler, Ruth Jesujobalayemi Awodipe, Poppy Cliffe, Nivethanaa Pulavan, Huba L Marton, Simon Gibbons, Michelle M C Buckner","doi":"10.1099/mic.0.001496","DOIUrl":"10.1099/mic.0.001496","url":null,"abstract":"Antimicrobial resistance (AMR) poses a significant threat to global public health. Notably, resistance to carbapenem and extended-spectrum β-lactam antibiotics in Gram-negative bacteria is a major impediment to treating infections. Genes responsible for antibiotic resistance are frequently carried on plasmids, which can transfer between bacteria. Therefore, exploring strategies to prevent this transfer and the prevalence of AMR plasmids is timely and pertinent. Here, we show that certain natural product extracts and associated pure compounds can reduce the conjugation of AMR plasmids into new bacterial hosts. Using our established high-throughput fluorescence-based flow cytometry assay, we found that the natural products were more active in reducing transmission of the IncK extended-spectrum β-lactamase-encoding plasmid pCT in Escherichia coli EC958c, compared to Klebsiella pneumoniae Ecl8 carrying the IncFII carbapenemase-encoding plasmid pKpQIL. The exception was the natural product rottlerin, also active in K. pneumoniae. In classical conjugation assays, rottlerin also reduced the conjugation frequency of the IncFII bla NDM-1 carrying plasmid pCPE16_3 from a clinical K. pneumoniae isolate. Our data indicate that the natural products tested here, in their current molecular structure, reduced conjugation by a small amount, which is unlikely to achieve a large-scale reduction in AMR in bacterial populations. However, certain natural products like rottlerin could provide a foundation for further research into compounds with effective anti-plasmid activity.","PeriodicalId":49819,"journal":{"name":"Microbiology-Sgm","volume":"170 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541548/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142074386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial Primer: The catalytic biofilm matrix. 微生物入门：催化生物膜基质。

IF 2.6 4区生物学

Microbiology-Sgm Pub Date : 2024-08-01 DOI: 10.1099/mic.0.001497

Lise Goltermann, Shahab Shahryari, Morten Rybtke, Tim Tolker-Nielsen

引用次数: 0

Dual transcriptional inhibition of glutamate and alanine racemase is synergistic in Mycobacterium tuberculosis. 谷氨酸和丙氨酸消旋酶的双重转录抑制在结核分枝杆菌中具有协同作用。

IF 2.6 4区生物学

Microbiology-Sgm Pub Date : 2024-08-01 DOI: 10.1099/mic.0.001484

Matthew B McNeil, Gregory M Cook, Kurt L Krause

引用次数: 0

Genomic characterization of prophage elements in Clostridium clostridioforme: an understudied component of the intestinal microbiome. 梭状芽孢杆菌(Clostridium clostridioforme)中噬菌体元素的基因组特征：肠道微生物组中未被充分研究的组成部分。

IF 2.6 4区生物学

Microbiology-Sgm Pub Date : 2024-08-01 DOI: 10.1099/mic.0.001486

Suzanne Humphrey, Angeliki Marouli, Katja Thümmler, Margaret Mullin, Leighton Pritchard, Daniel M Wall

{"title":"Genomic characterization of prophage elements in Clostridium clostridioforme: an understudied component of the intestinal microbiome.","authors":"Suzanne Humphrey, Angeliki Marouli, Katja Thümmler, Margaret Mullin, Leighton Pritchard, Daniel M Wall","doi":"10.1099/mic.0.001486","DOIUrl":"10.1099/mic.0.001486","url":null,"abstract":"Genome sequencing of Clostridium clostridioforme strain LM41 revealed the presence of an atypically high proportion of mobile genetic elements for this species, with a particularly high abundance of prophages. Bioinformatic analysis of prophage sequences sought to characterize these elements and identify prophage-linked genes contributing to enhanced fitness of the host bacteria in the dysbiotic gut. Using PHASTER, PhageScope and manual curation, this work has identified 15 prophages: 4 predicted to be intact, 2 predicted to be defective and 9 which are unclassified. Quantitative PCR (qPCR) analysis revealed spontaneous release of four of the LM41 prophages (φ1, φ2, φ4 and φ10) into the culture supernatant, with virion-like particles visualized using transmission electron microscopy. The majority (12/14) of these particles had morphology akin to podoviruses, which is consistent with morphology predictions for φ1 and φ4. We observed diversity in the lysogeny mechanisms utilized by the prophages, with examples of the classical λ-like CI/Cro system, the ICEBs1 ImmR/ImmA-like system and the Mu-like C/Ner system. Classical morons, such as toxins or immune evasion factors, were not observed. We did, however, identify a variety of genes with roles in mediating restriction modification and genetic diversity, as well as some candidate genes with potential roles in host adaptation. Despite being the most abundant entities in the intestine, there is a dearth of information about phages associated with members of the microbiome. This work begins to shed light on the contribution of these elements to the lifestyle of C. clostridioforme LM41.","PeriodicalId":49819,"journal":{"name":"Microbiology-Sgm","volume":"170 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11318856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141917946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity and structure of the deep-sea sponge microbiome in the equatorial Atlantic Ocean. 赤道大西洋深海海绵微生物群的多样性和结构。

IF 2.6 4区生物学

Microbiology-Sgm Pub Date : 2024-07-01 DOI: 10.1099/mic.0.001478

Sam E Williams, Gilda Varliero, Miguel Lurgi, James E M Stach, Paul R Race, Paul Curnow

{"title":"Diversity and structure of the deep-sea sponge microbiome in the equatorial Atlantic Ocean.","authors":"Sam E Williams, Gilda Varliero, Miguel Lurgi, James E M Stach, Paul R Race, Paul Curnow","doi":"10.1099/mic.0.001478","DOIUrl":"10.1099/mic.0.001478","url":null,"abstract":"Sponges (phylum Porifera) harbour specific microbial communities that drive the ecology and evolution of the host. Understanding the structure and dynamics of these communities is emerging as a primary focus in marine microbial ecology research. Much of the work to date has focused on sponges from warm and shallow coastal waters, while sponges from the deep ocean remain less well studied. Here, we present a metataxonomic analysis of the microbial consortia associated with 23 individual deep-sea sponges. We identify a high abundance of archaea relative to bacteria across these communities, with certain sponge microbiomes comprising more than 90 % archaea. Specifically, the archaeal family Nitrosopumilaceae is prolific, comprising over 99 % of all archaeal reads. Our analysis revealed that sponge microbial communities reflect the host sponge phylogeny, indicating a key role for host taxonomy in defining microbiome composition. Our work confirms the contribution of both evolutionary and environmental processes to the composition of microbial communities in deep-sea sponges.","PeriodicalId":49819,"journal":{"name":"Microbiology-Sgm","volume":"170 7","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11286294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141789657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0