Microbiology-Sgm最新文献_第10页

An assessment of the airborne longevity of group A Streptococcus. 评估 A 群链球菌在空气中的寿命。

IF 2.6 4区生物学

Microbiology-Sgm Pub Date : 2024-01-01 DOI: 10.1099/mic.0.001421

Henry P Oswin, Evie Blake, Allen E Haddrell, Adam Finn, Shiranee Sriskandan, Jonathan P Reid, Alice Halliday, Anu Goenka

{"title":"An assessment of the airborne longevity of group A Streptococcus.","authors":"Henry P Oswin, Evie Blake, Allen E Haddrell, Adam Finn, Shiranee Sriskandan, Jonathan P Reid, Alice Halliday, Anu Goenka","doi":"10.1099/mic.0.001421","DOIUrl":"10.1099/mic.0.001421","url":null,"abstract":"Group A streptococcus (GAS) infections result in more than 500 000 deaths annually. Despite mounting evidence for airborne transmission of GAS, little is known about its stability in aerosol. Measurements of GAS airborne stability were carried out using the Controlled Electrodynamic Levitation and Extraction of Bioaerosols onto a Substrate (CELEBS) instrument. CELEBS measurements with two different isolates of GAS suggest that it is aerostable, with approximately 70 % of bacteria remaining viable after 20 min of levitation at 50 % relative humidity (RH), with lower survival as RH was reduced. GAS airborne viability loss was driven primarily by desiccation and efflorescence (i.e. salt crystallization), with high pH also potentially playing a role, given reduced survival in bicarbonate containing droplet compositions. At low enough RH for efflorescence to occur, a greater proportion of organic components in the droplet appeared to protect the bacteria from efflorescence. These first insights into the aerosol stability of GAS indicate that airborne transmission of these respiratory tract bacteria may occur, and that both the composition of the droplet containing the bacteria, and the RH of the air affect the duration of bacterial survival in this environment. Future studies will explore a broader range of droplet and air compositions and include a larger selection of GAS strains.","PeriodicalId":49819,"journal":{"name":"Microbiology-Sgm","volume":"170 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10866022/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139099081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction and characterisation of a structured, tuneable, and transparent 3D culture platform for soil bacteria. 构建结构化、可调节和透明的土壤细菌三维培养平台并确定其特性。

IF 2.8 4区生物学

Microbiology-Sgm Pub Date : 2024-01-01 DOI: 10.1099/mic.0.001429

Liam M Rooney, Lionel X Dupuy, Paul A Hoskisson, Gail McConnell

引用次数: 0

Genomic and functional insights into antibiotic resistance genes floR and strA linked with the SXT element of Vibrio cholerae non-O1/non-O139. 与霍乱弧菌非 O1/ 非 O139 的 SXT 基因相连的抗生素耐药基因 floR 和 strA 的基因组和功能研究。

IF 2.8 4区生物学

Microbiology-Sgm Pub Date : 2024-01-01 DOI: 10.1099/mic.0.001424

Mousumi Saha, Agila Kumari Pragasam, Shashi Kumari, Jyoti Verma, Bhabatosh Das, Rupak K Bhadra

{"title":"Genomic and functional insights into antibiotic resistance genes floR and strA linked with the SXT element of Vibrio cholerae non-O1/non-O139.","authors":"Mousumi Saha, Agila Kumari Pragasam, Shashi Kumari, Jyoti Verma, Bhabatosh Das, Rupak K Bhadra","doi":"10.1099/mic.0.001424","DOIUrl":"10.1099/mic.0.001424","url":null,"abstract":"The emergence and spread of antibiotic-resistant bacterial pathogens are a critical public health concern across the globe. Mobile genetic elements (MGEs) play an important role in the horizontal acquisition of antimicrobial resistance genes (ARGs) in bacteria. In this study, we have decoded the whole genome sequences of multidrug-resistant Vibrio cholerae clinical isolates carrying the ARG-linked SXT, an integrative and conjugative element, in their large chromosomes. As in others, the SXT element has been found integrated into the 5'-end of the prfC gene (which encodes peptide chain release factor 3 involved in translational regulation) on the large chromosome of V. cholerae non-O1/non-O139 strains. Further, we demonstrate the functionality of SXT-linked floR and strAB genes, which confer resistance to chloramphenicol and streptomycin, respectively. The floR gene-encoded protein FloR belongs to the major facilitator superfamily efflux transporter containing 12 transmembrane domains (TMDs). Deletion analysis confirmed that even a single TMD of FloR is critical for the export function of chloramphenicol. The floR gene has two putative promoters, P1 and P2. Sequential deletions reveal that P2 is responsible for the expression of the floR. Deletion analysis of the N- and/or C-terminal coding regions of strA established their importance for conferring resistance against streptomycin. Interestingly, qPCR analysis of the floR and strA genes indicated that both of the genes are constitutively expressed in V. cholerae cells. Further, whole genome-based global phylogeography confirmed the presence of the integrative and conjugative element SXT in non-O1/non-O139 strains despite being non-multidrug resistant by lacking antimicrobial resistance (AMR) gene cassettes, which needs monitoring.","PeriodicalId":49819,"journal":{"name":"Microbiology-Sgm","volume":"170 1","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10866021/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139099082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meningeal Metastasis Causing Chronic Subdural Hematoma in a Cancer Patient with Bilateral Papilledema and Suspected Cerebral Venous Thrombosis: A Case Report. 一名双侧乳头水肿并疑似脑静脉血栓形成的癌症患者脑膜转移导致慢性硬膜下血肿：病例报告。

IF 1 4区生物学

Microbiology-Sgm Pub Date : 2024-01-01 Epub Date: 2022-04-22 DOI: 10.1055/a-1832-3598

Cornelia Pangratz-Daller, Jochen Grimm, Johannes A R Pfaff, Theo F J Kraus, Karl Sotlar, Abdul Rahman Al-Schameri, Michael Kral, Christoph J Griessenauer, Christoph Schwartz

引用次数: 0

A toolbox for manipulating the genome of the major goat pathogen, Mycoplasma capricolum subsp. capripneumoniae. 操纵山羊主要病原体冠突支原体冠突肺炎亚种基因组的工具箱。

IF 2.8 4区生物学

Microbiology-Sgm Pub Date : 2024-01-01 DOI: 10.1099/mic.0.001423

Géraldine Gourgues, Lucía Manso-Silván, Catherine Chamberland, Pascal Sirand-Pugnet, François Thiaucourt, Alain Blanchard, Vincent Baby, Carole Lartigue

{"title":"A toolbox for manipulating the genome of the major goat pathogen, Mycoplasma capricolum subsp. capripneumoniae.","authors":"Géraldine Gourgues, Lucía Manso-Silván, Catherine Chamberland, Pascal Sirand-Pugnet, François Thiaucourt, Alain Blanchard, Vincent Baby, Carole Lartigue","doi":"10.1099/mic.0.001423","DOIUrl":"10.1099/mic.0.001423","url":null,"abstract":"Mycoplasma capricolum subspecies capripneumoniae (Mccp) is the causative agent of contagious caprine pleuropneumonia (CCPP), a devastating disease listed by the World Organisation for Animal Health (WOAH) as a notifiable disease and threatening goat production in Africa and Asia. Although a few commercial inactivated vaccines are available, they do not comply with WOAH standards and there are serious doubts regarding their efficacy. One of the limiting factors to comprehend the molecular pathogenesis of CCPP and develop improved vaccines has been the lack of tools for Mccp genome engineering. In this work, key synthetic biology techniques recently developed for closely related mycoplasmas were adapted to Mccp. CReasPy-Cloning was used to simultaneously clone and engineer the Mccp genome in yeast, prior to whole-genome transplantation into M. capricolum subsp. capricolum recipient cells. This approach was used to knock out an S41 serine protease gene recently identified as a potential virulence factor, leading to the generation of the first site-specific Mccp mutants. The Cre-lox recombination system was then applied to remove all DNA sequences added during genome engineering. Finally, the resulting unmarked S41 serine protease mutants were validated by whole-genome sequencing and their non-caseinolytic phenotype was confirmed by casein digestion assay on milk agar. The synthetic biology tools that have been successfully implemented in Mccp allow the addition and removal of genes and other genetic features for the construction of seamless targeted mutants at ease, which will pave the way for both the identification of key pathogenicity determinants of Mccp and the rational design of novel, improved vaccines for the control of CCPP.","PeriodicalId":49819,"journal":{"name":"Microbiology-Sgm","volume":"170 1","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10866025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139404998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial Primer: In vivo biofilm. 微生物入门：体内生物膜。

IF 2.8 4区生物学

Microbiology-Sgm Pub Date : 2023-12-01 DOI: 10.1099/mic.0.001407

Kendra P Rumbaugh, Thomas Bjarnsholt

引用次数: 0

Microbe Profile: The Lactobacillaceae. 微生物简介：乳酸菌科

IF 2.8 4区生物学

Microbiology-Sgm Pub Date : 2023-12-01 DOI: 10.1099/mic.0.001414

Jens Walter, Paul W O'Toole

引用次数: 0

Atomistic modelling and NMR studies reveal that gallium can target the ferric PQS uptake system in P. aeruginosa biofilms. 原子模型和核磁共振研究显示，镓可以靶向铜绿微囊藻生物膜中的铁质 PQS 吸收系统。

IF 2.8 4区生物学

Microbiology-Sgm Pub Date : 2023-12-01 DOI: 10.1099/mic.0.001422

Oliver J Hills, Isaac O K Noble, Alex Heyam, Andrew J Scott, James Smith, Helen F Chappell

{"title":"Atomistic modelling and NMR studies reveal that gallium can target the ferric PQS uptake system in P. aeruginosa biofilms.","authors":"Oliver J Hills, Isaac O K Noble, Alex Heyam, Andrew J Scott, James Smith, Helen F Chappell","doi":"10.1099/mic.0.001422","DOIUrl":"10.1099/mic.0.001422","url":null,"abstract":"Intravenous gallium nitrate therapy is a novel therapeutic strategy deployed to combat chronic Pseudomonas aeruginosa biofilm infections in the lungs of cystic fibrosis (CF) patients by interfering with iron (Fe3+) uptake. The therapy is a source of Ga3+, which competes with Fe3+ for siderophore binding, subsequently disrupting iron metabolism and inhibiting biofilm proliferation in vivo. It was recently demonstrated that the Pseudomonas quinolone signal (PQS) can chelate Fe3+ to assist in bacterial iron uptake. However, it is unknown whether exogenous gallium also targets [Fe(PQS)3] uptake, which, in turn, would extend the mechanism of gallium therapy beyond siderophore competition, potentially supporting use of the therapy against P. aeruginosa mutants deficient in siderophore uptake proteins. To that end, the thermodynamic feasibility of iron-for-gallium cation exchange into [Fe(PQS)3] was evaluated using quantum chemical density functional theory (DFT) modelling and verified experimentally using 1H nuclear magnetic resonance (NMR). We demonstrate here that Ga3+ can strongly bind to three PQS molecules and, furthermore, displace and substitute Fe3+ from the native chelate pocket within PQS complexes, through a Trojan horse mechanism, retaining the key structural features present within the native ferric complex. As such, [Fe(PQS)3] complexes, in addition to ferric-siderophore complexes, represent another target for gallium therapy.","PeriodicalId":49819,"journal":{"name":"Microbiology-Sgm","volume":"169 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10765035/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138806790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interbacterial competition mediated by the type VIIb secretion system. 由 VIIb 型分泌系统介导的细菌间竞争。

IF 2.6 4区生物学

Microbiology-Sgm Pub Date : 2023-12-01 DOI: 10.1099/mic.0.001420

Eleanor R Boardman, Tracy Palmer, Felicity Alcock

引用次数: 0

A single shell protein plays a major role in choline transport across the shell of the choline utilization microcompartment of Escherichia coli 536. 单个壳蛋白在大肠杆菌536胆碱利用微室的胆碱转运中起主要作用。

IF 2.8 4区生物学

Microbiology-Sgm Pub Date : 2023-11-01 DOI: 10.1099/mic.0.001413

Jessica M Ochoa, Philip Dershwitz, Mary Schappert, Sharmistha Sinha, Taylor I Herring, Todd O Yeates, Thomas A Bobik

{"title":"A single shell protein plays a major role in choline transport across the shell of the choline utilization microcompartment of Escherichia coli 536.","authors":"Jessica M Ochoa, Philip Dershwitz, Mary Schappert, Sharmistha Sinha, Taylor I Herring, Todd O Yeates, Thomas A Bobik","doi":"10.1099/mic.0.001413","DOIUrl":"10.1099/mic.0.001413","url":null,"abstract":"Bacterial microcompartments (MCPs) are widespread protein-based organelles that play important roles in the global carbon cycle and in the physiology of diverse bacteria, including a number of pathogens. MCPs consist of metabolic enzymes encapsulated within a protein shell. The main roles of MCPs are to concentrate enzymes together with their substrates (to increase reaction rates) and to sequester harmful metabolic intermediates. Prior studies indicate that MCPs have a selectively permeable protein shell, but the mechanisms that allow selective transport across the shell are not fully understood. Here we examine transport across the shell of the choline utilization (Cut) MCP of Escherichia coli 536, which has not been studied before. The shell of the Cut MCP is unusual in consisting of one pentameric and four hexameric bacterial microcompartment (BMC) domain proteins. It lacks trimeric shell proteins, which are thought to be required for the transport of larger substrates and enzymatic cofactors. In addition, its four hexameric BMC domain proteins are very similar in amino acid sequence. This raises questions about how the Cut MCP mediates the selective transport of the substrate, products and cofactors of choline metabolism. In this report, site-directed mutagenesis is used to modify the central pores (the main transport channels) of all four Cut BMC hexamers to assess their transport roles. Our findings indicate that a single shell protein, CmcB, plays the major role in choline transport across the shell of the Cut MCP and that the electrostatic properties of the CmcB pore also impact choline transport. The implications of these findings with regard to the higher-order structure of MCPs are discussed.","PeriodicalId":49819,"journal":{"name":"Microbiology-Sgm","volume":"169 11","pages":""},"PeriodicalIF":2.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10710832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136400050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0