Pedobiologia最新文献

{"title":"Differential effects of urbanization-induced heavy metal pollution on soil microbial communities under evergreen and deciduous trees","authors":"Hao Hu ,&nbsp;Laiye Qu ,&nbsp;Siqi Tao ,&nbsp;Naili Zhang","doi":"10.1016/j.pedobi.2024.150999","DOIUrl":"10.1016/j.pedobi.2024.150999","url":null,"abstract":"<div><div>Urbanization has significantly increased heavy metal contamination in urban soils, adversely affecting soil microorganisms, which are vital indicators of soil quality. However, the effects of urbanization-induced metal pollution on soil microbial communities remains largely underestimated. This study examines soil microbial communities and properties beneath the canopy of three deciduous and three evergreen trees in urban parks, situated at varying distances from the city center. The results demonstrated that urbanization consistently alters soil physicochemical properties, including pH, soil moisture, and specific heavy metal contents (e.g., Zn, Mn, Cr). The α-diversity of soil bacterial community was significantly influenced by pH and specific heavy metals (e.g., Cr, Cd), whereas the α-diversity of fungal community was affected by pH, independent of heavy metal concentrations. The response of heavy metal content to urbanization exhibited a consistent pattern across both deciduous and evergreen trees, although the effect differed between these tree types. Furthermore, urbanization impacts the diversity, structure, composition and network of soil microbial communities. Notably, the Shannon index of soil fungal communities under deciduous species shows an initial increase, followed by a decline as urbanization intensifies. In contrast, the Simpson index of soil bacteria under evergreen tree species decreases with increased urbanization. Moreover, urbanization alters soil bacterial networks, with higher network density observed in less urbanized areas. It may also affect microbial functions, such as xenobiotic and lipid metabolism. This study provided a theoretical basis for urban park soil management, which is crucial for enhancing urban soil ecosystem services and mitigating the adverse effects of urbanization.</div></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"107 ","pages":"Article 150999"},"PeriodicalIF":2.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142421348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of microbial diversity in various saline soils driven by salt content","authors":"Dan Liu ,&nbsp;Panpan Gao ,&nbsp;Jiahe Niu ,&nbsp;Zhaoqi Qu ,&nbsp;Songnian Guo ,&nbsp;Chenxiao Ding ,&nbsp;Yanhong Lou ,&nbsp;Quangang Yang ,&nbsp;Hui Wang ,&nbsp;Zhongchen Yang ,&nbsp;Hongjie Di ,&nbsp;Hong Pan ,&nbsp;Yuping Zhuge","doi":"10.1016/j.pedobi.2024.150997","DOIUrl":"10.1016/j.pedobi.2024.150997","url":null,"abstract":"<div><p>The Yellow River Delta, as an important reserve land resource area, faces soil salinization problems. Understanding the bacterial community composition in saline soils is an important foundation for control and utilization of saline soils. However, few studies have been conducted on the composition of bacterial communities in soils with different degrees of salinization. Thus, saline soils categorized into low-salinity (LS), medium-salinity (MS), and high-salinity (HS) based on electrical conductivity (EC) were collected. The 16S rRNA high-throughput sequencing analysis was performed to analyze the effects of salinities on soil bacterial community patterns, as well as the relationships between soil bacterial communities and environmental factors. The results showed that Actinobacteriota, Proteobacteria, Chloroflexi, Firmicutes, Acidobacteriota, Gemmatimonadota and Bacteroidota accounted for almost 90 % of all the bacterial community. The linear discriminant analysis effects (LDA &gt; 3.7) showed that 6, 5 and 3 biomarkers were present in LS, MS and HS soils, respectively, which indicated EC was an important factor influencing the saline soil bacterial community patterns. Redundancy analysis further revealed that the primary environmental parameters impacting the bacterial community were pH, EC, nitrate nitrogen, available phosphorus, total phosphorus, and soil organic matter. According to network analysis, the microbial network complexity was increased steadily with increasing of soil salinity. These findings together revealed that bacterial communities could serve as a reliable way to assess and improve the quality of salinized soils.</p></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"107 ","pages":"Article 150997"},"PeriodicalIF":2.0,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142169341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing extraradical mycelium of mycorrhizal fungi in tropical forests using armored in-growth mesh bags","authors":"Andrey G. Zuev ,&nbsp;Ivan V. Gruzdev ,&nbsp;Anton M. Potapov ,&nbsp;Ina Schaefer ,&nbsp;Stefan Scheu ,&nbsp;Alexei V. Tiunov ,&nbsp;Nguyen Van Thinh ,&nbsp;Anna I. Zueva","doi":"10.1016/j.pedobi.2024.150989","DOIUrl":"10.1016/j.pedobi.2024.150989","url":null,"abstract":"<div><p>The extraradical mycelium of mycorrhizal fungi is among the major carbon pools in soil that is hard to quantitatively assess in-situ. Established method of in-growth mesh bags in temperate ecosystems is difficult to apply in the tropics, where mesh bags are often damaged by termites. Here we introduce a modification of the in-growth mesh bag technique, in which mesh bags are enforced by stainless steel mesh. Its performance was tested in the Đồng Nai (Cát Tiên) National Park in Vietnam across two monsoon tropical forests, dominated by tree species associated with either ectomycorrhizal (ECM) or arbuscular mycorrhizal (AM) fungi. Armored in-growth mesh bags remained intact, while about 60 % of non-armored mesh bags were damaged by termites after 180 days of exposure. The biomass of extraradical mycelium of ectomycorrhizal fungi estimated by PLFA analysis was similar in the armored and non-armored mesh bags and did not differ between studied forests. However, fungal community composition slightly differed between armored and non-armored mesh bags in the ECM- but not in the AM-dominated forest. Fungal mycelium gathered in the AM-dominated forest was depleted in ¹⁵N compared to that collected in the ECM-dominated forest. Overall, our results argue for using armored mesh bags as a robust tool for harvesting the biomass of extraradical mycelium of mycorrhizal fungi in tropical ecosystems.</p></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"106 ","pages":"Article 150989"},"PeriodicalIF":2.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142099403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in soil arthropods and litter nutrients after prescribed burn in a subtropical moist pastureland","authors":"Wei Huang ,&nbsp;Grizelle González ,&nbsp;María Fernanda Barberena-Arias ,&nbsp;Weili Liu ,&nbsp;Xiaoming Zou","doi":"10.1016/j.pedobi.2024.150990","DOIUrl":"10.1016/j.pedobi.2024.150990","url":null,"abstract":"<div><p>Plant litter decomposition is driven by soil biota and biophysiochemical conditions as well as substrate quality. Prescribed burns can affect the abundance and diversity of soil arthropods and the biophysiochemical conditions in terrestrial ecosystems. In this study, we examined the effects of a prescribed burn on soil arthropods and litter chemistry in decomposing litter during a total of 469-days field incubation using litter from two grasses, <em>Dichanthium annulatum</em> and <em>Megathyrsus maximus</em>, in a subtropical moist pastureland of Puerto Rico. We found the prescribed burn substantially elevated ultraviolet (UV) radiation and soil temperature; and significantly decreased the diversity of litter total arthropods, especially predators and Mesostigmata mites, during the initial 5 months after the burn. However, the prescribed burn had no effect on either the biophysical environment nor on arthropod abundance and diversity during the subsequent incubation period of &gt;5 months after the burn. Furthermore, the prescribed burn substantially increased the immobilization of iron (Fe) and manganese (Mn), and decreased sulfur (S) concentration in the decomposing litter. Prescribed burn had no interactions with substrate quality for percent mass remaining (PMR) and elemental release or accumulation. Low substrate quality <em>D. annulatum</em> litter with a carbon to phosphorus (C/P) ratio of 614 was associated with higher microbivore diversity and higher predator density than higher substrate quality <em>M. maximus</em> litter with a C/P ratio of 266 during the entire incubation period. Lower initial concentration of litter P, magnesium (Mg) and calcium (Ca) in <em>D. annulatum</em> resulted in higher immobilization of these elements in decomposing litter than in <em>M. maximus</em>. Our study suggest that prescribed burn can impose short-term changes in biophysiochemical conditions and the diversity of arthropods in litter decomposition during the initial recovery period of about 5 months after a burn, thus highlighting a high resilience of the grassland ecosystem to fire disturbance, and that it can bring lasting changes in the cycling of Fe, Mn, and S in subtropical moist pastureland that can alter ecosystem productivity.</p></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"106 ","pages":"Article 150990"},"PeriodicalIF":2.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142128781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collembola and ants: Influence of trails of red wood ants (Formica lugubris) on the community of soil springtails","authors":"Danila N. Goryunov ,&nbsp;Ksenia S. Panina ,&nbsp;Anna I. Bokova","doi":"10.1016/j.pedobi.2024.150987","DOIUrl":"10.1016/j.pedobi.2024.150987","url":null,"abstract":"<div><p>Red wood ants are keystone species in forest ecosystems. Because ants are active polyphagous predators, they control the abundance of other arthropods. In addition to direct effects on other species, there are a number of indirect effects caused by ants. In our study, we investigated the influence of ants on springtails, which they rarely come into contact with. Springtails have been set as a model group to assess the state of soil animals, since they are one of the most numerous and widespread groups of soil microarthropods. They are characterized by high sensitivity to environmental changes. The basic characteristics (abundance, species richness, and species diversity) of the springtail community and the response of certain species to the presence of ant trails have been studied. The total abundance and species richness of springtails decreased along ant trails. Among occurred species <em>Parisotoma ParIsotoma notabilis, Lepidacyrtus lignorum, Isotomiella minor, Desoria tigrina</em>, and <em>Pseudasinella alba</em> are the most numerous species both along ant trails and in control samples<em>.</em> There are no significant changes in species diversity (assessed by the Shannon-Weaver index). Species structure of springtail communities along ant trails and in control is different. Two groups of common Collembola species are identified: (1) increasing their numbers in the presence of ants, (2) on the contrary, decreasing. The presence of ants in a forest ecosystem influences the soil springtail community, although ant-Collembola interactions are indirect.</p></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"106 ","pages":"Article 150987"},"PeriodicalIF":2.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142099563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential of soil endemic microorganisms in ameliorating the physicochemical properties of soil subjected to a freeze-thaw cycle","authors":"Sudabeh Gharemahmudli ,&nbsp;Seyed Hamidreza Sadeghi ,&nbsp;Ali Najafinejad","doi":"10.1016/j.pedobi.2024.150988","DOIUrl":"10.1016/j.pedobi.2024.150988","url":null,"abstract":"<div><p>Establishing soil biological crusts will result in the long-term restoration of ecosystems. Nonetheless, little research has been conducted to demonstrate the influence of soil endemic microorganisms on suppressing the adverse effects of freezing-thawing on soil properties. This current study evaluated the formation of biological crusts, the enhancement of physical and chemical characteristics of soil, and surface soil stability by inoculating native bacteria and cyanobacteria into the soil during a freezing-thawing cycle. The soil was collected from the Badranlou Region in North Khorasan Province, Northern Iran, and native bacteria and cyanobacteria were isolated, identified, chosen, and cultured. The native treatments of bacteria and cyanobacteria were then inoculated in individual bacteria, cyanobacteria, and combined inoculation of cyanobacteria and bacteria onto an experimental soil in six replications. After 60 days, they were subjected to freezing-thawing conditions to have maximum effect on the soil environment, and finally, the soil surface properties were statistically compared. The results of the significant effect (p&lt;0.001) of inoculation treatments on the physical and chemical properties of the study soil revealed that the carbon, nitrogen, organic matter, phosphorus, potassium, and surface soil stability in bacterial treatment compared to the control treatment increased by 68, 39, 68, 17, 25, 99 %, respectively. While under cyanobacterial treatment, they rose by 83, 61, 83, 18, 73, and 172 %, respectively. The combination inoculation treatment of bacteria and cyanobacteria enhanced the study variables by 73, 66, 73, 25, 58, and 189 %, respectively. Compared to control plots, the soil bulk density in bacterial, cyanobacterial, and compound inoculation treatments was substantially reduced (p&lt;0.001) by 9, 15, and 12 %, respectively. The soil stability, carbon, and organic matter were among the most essential properties of the soil, and they best showed the difference between the various treatments applied. It confirmed the region's potential restoration by inoculating native soil microorganisms.</p></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"106 ","pages":"Article 150988"},"PeriodicalIF":2.0,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142084132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Winter cover crops and irrigation alter soil microbial community composition in an arid cropping system","authors":"Erik A. Lehnhoff ,&nbsp;Caroline R. Toth ,&nbsp;Prashasti Agarwal ,&nbsp;Ciro Velasco-Cruz ,&nbsp;Brian J. Schutte ,&nbsp;Omololu John Idowu ,&nbsp;Xiufen Li","doi":"10.1016/j.pedobi.2024.150986","DOIUrl":"10.1016/j.pedobi.2024.150986","url":null,"abstract":"<div><p>Cover cropping is a well-established strategy to improve soil health, especially in arid and semi-arid agricultural systems. Benefits to soil health are often mediated via effects of cover crops on soil microbial community structure, function and diversity, crucial for regulating soil biogeochemical cycles, eventually promoting agricultural sustainability. However, limited water availability is a major constraint for both cover crop growth and soil microbial activity. This study sought to characterize and elucidate the shifts in soil microbial community structure in response to different cover crops and differential irrigation treatments using phospholipid fatty acid (PLFA) profiling in southern New Mexico. We tested five cover crop treatments: <em>Pisum sativum</em> (Australian winter pea), <em>Hordeum vulgare</em> cv. Stockford (barley), <em>Brassica juncea</em> cv. Caliente 199 (brown mustard), a three-way mix, and a fallow control — in combination with irrigation treatments of one, two, or three irrigation applications — in a split-plot design over two years. <em>Zea mays</em> (sweet corn) was grown as the summer cash crop. We collected soil samples just after cover crop planting in the fall of 2018, following second year cover crop termination but during <em>Z. mays</em> growth in June 2020, and after the second season of <em>Z. mays</em> growth in October 2020. Differential irrigation treatments did not lead to consistent patterns of change under any cover crop or irrigation treatment. However, PLFA parameters in cover cropped compared to winter fallow plots tended to decrease under one and three irrigations but increased with two irrigations. Changes were more common for bacterial than for fungal PLFA biomarkers, and more common in <em>B. juncea</em> and <em>H. vulgare</em> cover crops than in <em>P. sativa</em> or the mix. It is important to note that, while cover crop effects were inconsistent, cover cropping did lead to some shifts in PLFA biomarkers, even in the short two-year period of cover cropping.</p></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"106 ","pages":"Article 150986"},"PeriodicalIF":2.0,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141961434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Different composition of plant residues as a driver of microbial community structure and soil organic matter composition: A microcosm study","authors":"Anacláudia Alves Primo ,&nbsp;José Ferreira Lustosa Filho ,&nbsp;Helen Botelho Marota ,&nbsp;Rafael Gonçalves Tonucci ,&nbsp;Ivo Ribeiro da Silva ,&nbsp;Teogenes Senna de Oliveira","doi":"10.1016/j.pedobi.2024.150985","DOIUrl":"10.1016/j.pedobi.2024.150985","url":null,"abstract":"<div><p>Soil organic matter (SOM) is the main pathway of carbon (C) input to the soil with the decomposition of shoot residues, roots and their exudates. The objective was to evaluate the contribution of different vegetal composition and plant parts of Caatinga species and the effects of introducing a grass in the soil microbial community structure and biochemical composition of SOM. A trial was conducted under controlled conditions (120 days) using, separately, the shoot and roots residue<strong>s</strong> of native species from the herbaceous (HERB) and shrub-arboreal (ARB) strata and a grass (GRASS). <em>Megathyrsus maximum</em>, which is native from Africa, but well adapted to the semi-arid conditions of Brazil, was used. Combinations of these species in different proportions were also evaluated: (i) 55 % shrub and trees + 45 % grass (MIX1) and (ii) 75 % shrub and trees + 25 % grass (MIX2). At the end of incubation, soil samples were collected to evaluate the microbial community structure through the phospholipid fatty acids (PLFA). Physical fractioning of SOM into particulate organic matter (POM) and mineral-associated organic matter (MAOM) was also performed, followed by biochemical characterization of these fractions by thermochemolysis analysis. The ARB shoot residue resulted in a 181.5 % increase (<em>p</em> &lt; 0.05) in total PLFA biomass in the soil. A significant increase (<em>p</em> &lt; 0.05) in the abundance of fungi and bacteria was observed with the incorporation of shoot residues. MAOM was characterized by a higher abundance of aliphatic (31.6 ± 5.0 %) and nitrogen-bearing compounds (21.0 ± 2.0 %), while higher lignin derivatives were observed in POM (18.0 ± 0.6 %). The ground cover provided a diversity of compounds in the SOM, thus regulating the structure of the microbial community. These results highlight the importance of conserving biodiversity, both in natural ecosystems and in agroecosystems in the semi-arid environment.</p></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"106 ","pages":"Article 150985"},"PeriodicalIF":2.0,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141847816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Precise method to measure fungal and bacterial necromass using high pressure liquid chromatography with fluorescence detector adjusted to inorganic, organic and peat soils","authors":"Sylwia Adamczyk,&nbsp;Raisa Mäkipää,&nbsp;Aleksi Lehtonen,&nbsp;Bartosz Adamczyk","doi":"10.1016/j.pedobi.2024.150977","DOIUrl":"https://doi.org/10.1016/j.pedobi.2024.150977","url":null,"abstract":"<div><p>Soil organic matter is the dominant pool of carbon (C) in terrestrial ecosystems. Recent advances in understanding of the mechanisms of C stabilization in the soil emphasize microbes as the main drivers. Special attention is placed on the accumulation of bacterial and fungal necromasses. This calls for development of fast and reliable methods to estimate microbial necromass in a various type of soils, including peat soils. Here we provide precise method to measure fungal and bacterial necromasses with high-pressure liquid chromatography-fluorescence detector (HPLC-FLD) and its comparison with gas chromatography method. Purity of the chromatographic peaks was confirmed with mass spectrometry. The HPLC-FLD method provides reliable results for mineral, organic and highly organic peat soils.</p></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"106 ","pages":"Article 150977"},"PeriodicalIF":2.0,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S003140562403498X/pdfft?md5=9138454a3c41d0432368884a8768965c&pid=1-s2.0-S003140562403498X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141606968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological control of damping-off by Fusarium oxysporum and F. verticillioides on pine and oak seedlings using edible ectomycorrhizal fungi","authors":"Jorge Poveda,&nbsp;Jorge Martín-García,&nbsp;Paula Zamora-Brauweiler,&nbsp;Mónica Pastor,&nbsp;Julio J. Díez","doi":"10.1016/j.pedobi.2024.150973","DOIUrl":"https://doi.org/10.1016/j.pedobi.2024.150973","url":null,"abstract":"<div><p>The Mediterranean forest has an important ecological and economic role, being holm oak (<em>Quercus ilex</em>), Pyrenean oak (<em>Quercus pyrenaica</em>), umbrella pine (<em>Pinus pinea</em>) and Scot pine (<em>Pinus sylvestris</em>) some of its main tree species. The fungal damping-off disease caused by <em>Fusarium</em> seriously threatens the establishment of these forest species in nurseries and reforestation, requiring the search for environmentally friendly alternatives to control the disease. We have used different species of ectomycorrhizal fungi (EMF) as potential biological control agents (BCAs) effective against the disease: <em>Lactarius sanguifluus</em>, <em>Tricholoma portentosum</em>, <em>Suillus luteus</em> and <em>Agaricus silvicola</em> from <em>Pinus</em>-species, and <em>Leccinum lepidum</em>, <em>Amanita rubescens</em> and <em>Xerocomus ferrugineus</em> from <em>Quercus</em>-species. A direct <em>in vitro</em> confrontation was performed and conidial germination of <em>Fusarium</em> in contact with cell-free filtrates produced by EMF was studied. <em>Le. lepidum</em> was the most effective <em>Quercus</em>-fungus <em>in vitro</em> against <em>F. oxysporum</em>, reducing its growth up to 32 % and its conidial germination up to 87 %. <em>S. luteus</em> was the most effective <em>Pinus</em>-fungus <em>in vitro</em> against <em>F. oxysporum</em> and <em>F. verticillioides</em>, reducing in direct confrontation, reducing its growth up to 30 %. However, <em>La. sanguifluus</em> was the <em>Pinus</em>-fungus that inhibited conidial germination of both pathogens, up to 55 %. <em>In planta</em> trials were carried out with seeds of the four forest species growing on substrate colonized by <em>Le. lepidum</em> (in <em>Q. ilex</em> and <em>Q. pyrenaica</em> seeds, infected by <em>F. oxysporum</em>) or by <em>La. sanguifluus</em> (<em>P. pinea</em> and <em>P. sylvestris</em> seeds, infected by <em>F. oxysporum</em> or <em>F. verticillioides</em>). Only <em>La. sanguifluus</em> was effective in reducing disease caused by <em>F. oxysporum</em> (strain Fo4) on <em>P. sylvestris</em> seeds. Therefore, EMF may be a potential tool in the control of damping-off in forest species, requiring further research.</p></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"105 ","pages":"Article 150973"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141479968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}