Precise method to measure fungal and bacterial necromass using high pressure liquid chromatography with fluorescence detector adjusted to inorganic, organic and peat soils

IF 2 3区农林科学 Q3 ECOLOGY

Pedobiologia Pub Date : 2024-07-10 DOI:10.1016/j.pedobi.2024.150977

Sylwia Adamczyk, Raisa Mäkipää, Aleksi Lehtonen, Bartosz Adamczyk

引用次数: 0

Abstract

Soil organic matter is the dominant pool of carbon (C) in terrestrial ecosystems. Recent advances in understanding of the mechanisms of C stabilization in the soil emphasize microbes as the main drivers. Special attention is placed on the accumulation of bacterial and fungal necromasses. This calls for development of fast and reliable methods to estimate microbial necromass in a various type of soils, including peat soils. Here we provide precise method to measure fungal and bacterial necromasses with high-pressure liquid chromatography-fluorescence detector (HPLC-FLD) and its comparison with gas chromatography method. Purity of the chromatographic peaks was confirmed with mass spectrometry. The HPLC-FLD method provides reliable results for mineral, organic and highly organic peat soils.

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利用带荧光检测器的高压液相色谱法测量真菌和细菌坏死物质的精确方法，适用于无机土、有机土和泥炭土

土壤有机质是陆地生态系统中最主要的碳库。最近，人们对土壤中碳稳定机制的认识有了新的进展，强调微生物是主要的驱动力。细菌和真菌新菌体的积累受到特别关注。这就需要开发快速可靠的方法来估算包括泥炭土在内的各类土壤中的微生物坏死物质。在此，我们提供了利用高压液相色谱-荧光检测器（HPLC-FLD）测量真菌和细菌坏死物的精确方法，并将其与气相色谱法进行了比较。质谱法确认了色谱峰的纯度。高压液相色谱-荧光检测器法可为矿物、有机和高有机泥炭土提供可靠的结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pedobiologia 环境科学-生态学

CiteScore

4.20

自引率

8.70%

发文量

审稿时长

64 days

期刊介绍： Pedobiologia publishes peer reviewed articles describing original work in the field of soil ecology, which includes the study of soil organisms and their interactions with factors in their biotic and abiotic environments. Analysis of biological structures, interactions, functions, and processes in soil is fundamental for understanding the dynamical nature of terrestrial ecosystems, a prerequisite for appropriate soil management. The scope of this journal consists of fundamental and applied aspects of soil ecology; key focal points include interactions among organisms in soil, organismal controls on soil processes, causes and consequences of soil biodiversity, and aboveground-belowground interactions. We publish: original research that tests clearly defined hypotheses addressing topics of current interest in soil ecology (including studies demonstrating nonsignificant effects); descriptions of novel methodological approaches, or evaluations of current approaches, that address a clear need in soil ecology research; innovative syntheses of the soil ecology literature, including metaanalyses, topical in depth reviews and short opinion/perspective pieces, and descriptions of original conceptual frameworks; and short notes reporting novel observations of ecological significance.