PedobiologiaPub Date : 2026-03-01Epub Date: 2025-12-22DOI: 10.1016/j.pedobi.2025.151110
Edward J. Primka IV , Eric B. Duell , Chris B. Zou , Gail W.T. Wilson
{"title":"Soil carbon and fungal functional group dynamics in forest-savanna transitions","authors":"Edward J. Primka IV , Eric B. Duell , Chris B. Zou , Gail W.T. Wilson","doi":"10.1016/j.pedobi.2025.151110","DOIUrl":"10.1016/j.pedobi.2025.151110","url":null,"abstract":"<div><div>Increasing soil organic carbon storage is of global interest due to its potential for removing CO<sub>2</sub> from the atmosphere. Savannas are well known for providing a multitude of diverse ecosystem services; however, the impacts of tree thinning and fire frequency during the forest to savanna conversion on soil organic carbon concentration (SOCc) and mycorrhizal fungal biomass remain poorly understood. This study, part of a long-term research project established in 1983 in southeastern Oklahoma, USA, examines how managed landscapes and resultant vegetation cover (i.e., forests and savannas) influence soil carbon storage and fungal functional group dynamics. Our findings reveal that in the top 5 cm of soil, SOCc was driven by soil macroaggregate abundance in forests and pH in savannas, with SOCc 23 % higher in forests, compared to savannas. At greater depths, pseudo-effective cation exchange capacity influenced SOCc in both cover types, with no significant differences in SOCc. Biomass of fungal functional groups (arbuscular mycorrhizal or ectomycorrhizal/saprophytic), assessed via phospholipid fatty acid analysis (PLFA), were largely driven by fire return interval. Additionally, total fungal biomass was predicted by the cation exchange capacity of the soil, regardless of cover type. Furthermore, despite the shift from ectomycorrhizal/saprophytic to arbuscular mycorrhizal fungi with forest to savanna conversion, savannas did not exhibit higher carbon concentration in the soil. Overall, our findings suggest that in the forest-grassland ecotone of the south-central USA, maintaining and preserving historic savannas that provide important ecosystem services requires only a small compromise in terms of soil carbon storage across the landscape.</div></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"114 ","pages":"Article 151110"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145841924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PedobiologiaPub Date : 2026-03-01Epub Date: 2025-12-12DOI: 10.1016/j.pedobi.2025.151109
Sékou F.M. Coulibaly , Juliette Chassain , Laure Vieublé-Gonod , Maxime Artru , Olivier De Carville , Swann Felin , Antoine Gardarin , Sophie Joimel
{"title":"Within-field flower strips effect on Collembola assemblages over time","authors":"Sékou F.M. Coulibaly , Juliette Chassain , Laure Vieublé-Gonod , Maxime Artru , Olivier De Carville , Swann Felin , Antoine Gardarin , Sophie Joimel","doi":"10.1016/j.pedobi.2025.151109","DOIUrl":"10.1016/j.pedobi.2025.151109","url":null,"abstract":"<div><div>For several decades, techniques aimed at maximizing agricultural production have been developing rapidly. This has led to an intensification of cultural practices and concerns regarding the conservation of soil biodiversity and ecological functions and services it supports. A major challenge of agroecology is to define new methods for managing agroecosystems that enable a sustainable soil use and the preservation of its biodiversity. Sowing flower strips is often promoted in agri-environmental schemes, but their effect on soil biodiversity has yet been poorly considered. In this study, we followed the Collembola assemblages in response to the establishment of perennial wild flower strips within eight arable fields over four years (2018–2022). Collembola were sampled in the fields before the installation of the flower strips, in 2018 (y + 0), and in 2019 (y + 1), 2021 (y + 3) and 2022 (y + 4). Soil samples were taken within, at 5 m and at 30 m from the flower strips. Our results showed that there were no significant differences between the collembolan assemblages depending on the sampling distances from the flower strips. However, the age of the flower strips had an effect on Collembola assemblages, with y + 1 showing the lowest Collembola density and diversity. The differentiation between Collembola assemblages occurred three years after the flower strips sowing and was maintained for up to four years. Collembola appear to respond to environmental changes caused by flower strips sowing, since response traits such as sensory organs, pigmentation and sexual reproduction were the most dominant.</div></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"114 ","pages":"Article 151109"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145754153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PedobiologiaPub Date : 2026-03-01Epub Date: 2026-01-05DOI: 10.1016/j.pedobi.2026.151112
Yue Qiu , Wei Ji , Sibei Chen , Guitong Li , Qimei Lin , Xiaorong Zhao , Owen Fenton , Hao Chen
{"title":"Divergent responses of soil bacterial and fungal biomass carbon and phosphorus (P) to a P addition gradient","authors":"Yue Qiu , Wei Ji , Sibei Chen , Guitong Li , Qimei Lin , Xiaorong Zhao , Owen Fenton , Hao Chen","doi":"10.1016/j.pedobi.2026.151112","DOIUrl":"10.1016/j.pedobi.2026.151112","url":null,"abstract":"<div><div>Microorganisms mediate element dynamics in soils through biomass turnover, and their activity is largely affected by nutrient inputs. However, as the two major subgroups of soil microbial community, little is known regarding how bacterial and fungal biomass carbon (C) and phosphorus (P) respond to a P addition gradient. Herein, the dynamic changes of bacterial and fungal biomass C and P from the soil in northern China were studied using the selective inhibition technique. A 20-d soil incubation experiment was set up with different P additions: 0, 20 and 60 mg kg<sup>−1</sup> soil. The experiment selected streptomycin (10 mg g<sup>−1</sup> soil) and cycloheximide (20 mg g<sup>−1</sup> soil) as bacterial and fungal inhibitors, respectively. The results showed that P addition did not affect the total microbial biomass C but substantially adjusted the composition contributed by bacteria and fungi. Fungal biomass C contributed highest at the low P addition rate, but its significance tended to decrease as P addition increased, whereas the contribution of bacterial biomass C increased. P addition positively affected both bacterial and fungal biomass P, but at a higher extent for bacteria. In terms of time, bacteria showed a higher potential to release P into soil labile P pools through biomass turnover at an earlier stage after P addition, whereas fungi may have a slow effect as sources of soil labile P. These results indicate divergent responses for bacterial and fungal biomass across different P additions. Such insights should be considered at field scale and should inform C and nutrient managements planning in terms of soil C and P cycling.</div></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"114 ","pages":"Article 151112"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PedobiologiaPub Date : 2026-03-01Epub Date: 2026-02-20DOI: 10.1016/j.pedobi.2026.151122
Kohsuke Tanigawa , Mika Lemoine , Floretta Setia Pradana , Sandra Jämtgård , Aimée T. Classen , David A. Wardle , Paul Kardol
{"title":"Effects of multi-level warming on soil nematodes and microarthropods in tundra heath mesocosms","authors":"Kohsuke Tanigawa , Mika Lemoine , Floretta Setia Pradana , Sandra Jämtgård , Aimée T. Classen , David A. Wardle , Paul Kardol","doi":"10.1016/j.pedobi.2026.151122","DOIUrl":"10.1016/j.pedobi.2026.151122","url":null,"abstract":"<div><div>Soil fauna, including nematodes and microarthropods (mites and springtails), regulate nutrient cycling and decomposition, yet their responses to warming remain unclear in temperature-sensitive tundra ecosystems. We experimentally warmed intact tundra heath monoliths from northern Sweden under five temperature treatments (ambient to +9 °C) for 20 weeks. Monoliths were dominated by either <em>Betula nana</em> or <em>Empetrum nigrum</em> subsp. <em>hermaphroditum</em>. After incubation, we quantified nematode and microarthropod densities and community composition. Warming altered both nematode and microarthropod communities, with responses mediated by vegetation type, trophic group, and taxon. Microarthropods densities were generally more sensitive to warming, especially under the dominance of <em>E. nigrum</em>, whereas nematode communities showed stronger compositional shifts. Overall nematode densities were stable across warming levels, except for fungivorous nematodes, which decreased linearly with warming. In contrast, total and carnivorous microarthropod densities increased linearly with warming, while springtails and fungivorous microarthropods displayed non-linear, U-shaped responses. Together, these results demonstrate that soil fauna responses to warming range from negligible to linear and non-linear, underscoring the importance of experimental designs that span multiple warming levels. The strong dependence of these responses on vegetation context and trophic identity emphasizes the need to account for local environmental heterogeneity when predicting tundra ecosystem responses to climate change.</div></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"114 ","pages":"Article 151122"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147396777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PedobiologiaPub Date : 2026-03-01Epub Date: 2026-01-23DOI: 10.1016/j.pedobi.2025.151111
Hiro Kasai , Takuo Sawahata
{"title":"Hidden collembolan forest habitat: Specificity and species diversity of Collembola communities on fallen branches","authors":"Hiro Kasai , Takuo Sawahata","doi":"10.1016/j.pedobi.2025.151111","DOIUrl":"10.1016/j.pedobi.2025.151111","url":null,"abstract":"<div><div>Collembola are an important class of arthropods in forest ecosystems. However, research on these organisms has traditionally focused on soil and litter layers while largely neglecting other potential habitats. Therefore, this study explored potential habitats of Collembola beyond those traditionally investigated. We hypothesized that fallen branches, which are widespread on the forest floor but have seldom been studied as habitats for collembolan communities, represent a potentially important microhabitat. We compared collembolan communities in leaf litter and soil and on fallen branches at three decomposition stages (early, middle, and late) in evergreen broad-leaved forests in the warm temperate zone of Japan. We found that Collembola communities hosted in middle- and late-stage fallen branches were more abundant and diverse than those in the soil and litter layers. In addition, Neanuridae, Hypogastruridae, and Odontellidae members mainly colonized fallen branches. These results indicate that the species diversity and community composition of Collembola differed significantly among vertical microhabitats, with the previously overlooked habitat of fallen branches supporting distinct Collembola communities and thus enhancing forest biodiversity.</div></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"114 ","pages":"Article 151111"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PedobiologiaPub Date : 2026-03-01Epub Date: 2026-02-09DOI: 10.1016/j.pedobi.2026.151119
Eric Kanold , Robert W. Buchkowski , William C. Parker , Eric B. Searle , Christian Messier , Carlos Barreto
{"title":"Tree species richness and identity influence the abundance of excysted soil ciliates in young forest plantations","authors":"Eric Kanold , Robert W. Buchkowski , William C. Parker , Eric B. Searle , Christian Messier , Carlos Barreto","doi":"10.1016/j.pedobi.2026.151119","DOIUrl":"10.1016/j.pedobi.2026.151119","url":null,"abstract":"<div><div>Soil protists play crucial roles in nutrient cycling, organic matter decomposition, and regulating belowground food webs, but relatively little is known about the effects of tree diversity on this microscopic group. We used the International Diversity Experiment Network with Trees (IDENT) experiment in Sault Ste. Marie, Canada to address the effects of tree species richness and functional diversity, and high and low growing season soil moisture treatments on soil ciliate abundance. In total, we found 2285 ciliates from 181 plots representing 22 tree communities and an unplanted control plot. Soil ciliate abundance varied significantly with species identity of tree communities, but no significant non-additive net mixing effects was found. Ciliate abundance was also significantly and negatively influenced by soil temperature on the day of sampling. We found a significant, albeit weak, positive relationship between tree species richness and total ciliate abundance that was driven primarily by <em>Paramecium</em> spp. Functional tree diversity had a similar relationship with ciliate abundance that was only marginally significant, while soil moisture treatment had no effect on abundance. These findings suggest that higher tree species richness in young plantations can have a positive influence on the abundance of soil ciliates, though the relationship is context-dependent, differing slightly with species identity of trees. Our study contributes to our current knowledge of tree diversity effects on soil ciliate abundance and highlights the need for further research to fully understand these complex relationships and their implications for forest ecosystem functioning.</div></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"114 ","pages":"Article 151119"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146173593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PedobiologiaPub Date : 2026-03-01Epub Date: 2026-02-03DOI: 10.1016/j.pedobi.2026.151116
Lige Zhang , Zi-Kai Liu , Yuhai Chang , Shengsheng Jin , Hongjie Di , Ju-Pei Shen
{"title":"Soil depth mediates contrasting effects of nitrogen and phosphorus inputs on microbial carbon use efficiency in a subtropical forest","authors":"Lige Zhang , Zi-Kai Liu , Yuhai Chang , Shengsheng Jin , Hongjie Di , Ju-Pei Shen","doi":"10.1016/j.pedobi.2026.151116","DOIUrl":"10.1016/j.pedobi.2026.151116","url":null,"abstract":"<div><div>Forest soils serve as vital terrestrial carbon sinks, in which microbial carbon use efficiency (CUE) plays a central role in regulating soil carbon storage. While anthropogenic deposition exerts profound influences on forest carbon cycling, the depth-dependent responses of microbial CUE to nitrogen (N) and phosphorus (P) deposition remain poorly understood. Here, we examined how soil microbial CUE responded to N and P inputs along a soil profile (0–80 cm) in a subtropical forest, using a long-term simulated N and P deposition experiment. Our results demonstrated a consistent increase in microbial CUE with depth across all treatments. Specifically, N addition significantly enhanced CUE by 27 % and 10 % at the depth of 40–60 cm and 60–80 cm, respectively, relative to the non-N group (which controlled for P variation). Furthermore, when compared directly to control, N addition increased CUE by 12.7 % and 13.5 % at these depths. In contrast, P addition suppressed CUE by 14 % and 16 % at the depth of 0–20 cm and 20–40 cm, respectively, compared to the non-P group (which controlled for N variation). Similarly, direct comparison with the control showed that P addition decreased CUE by 8.1 % and 10.9 % at these topsoil layers. Regression analyses revealed significant associations between microbial CUE and soil pH, dissolved organic C, dissolved organic N, and available P. Variance partitioning analysis further elucidated that microbial CUE was predominantly governed by the interplay of soil substrates (primarily carbon and nutrient availability) and microbial enzymatic activities in surface layers, and N input was a major environmental driver for CUE variation in deep soils. These findings uncover contrasting nutrient–depth interactions in regulating microbial CUE and underscore the pivotal role of subsoil C dynamics under escalating N deposition.</div></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"114 ","pages":"Article 151116"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146173738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PedobiologiaPub Date : 2026-03-01Epub Date: 2026-02-10DOI: 10.1016/j.pedobi.2026.151117
C. Cifuentes-Croquevielle , F. Machuca , R.M. Barahona-Segovia , F. Matus , A. Zúñiga-Feest
{"title":"Wildfire-induced changes in soil invertebrate communities and their link to litter decomposition in a high-elevation temperate forest","authors":"C. Cifuentes-Croquevielle , F. Machuca , R.M. Barahona-Segovia , F. Matus , A. Zúñiga-Feest","doi":"10.1016/j.pedobi.2026.151117","DOIUrl":"10.1016/j.pedobi.2026.151117","url":null,"abstract":"<div><div>The primary forests in southern Chile harbor unique endemic plants and diverse soil invertebrates that are vital for nutrient cycling. These forests face an atypical fire regime because fire is not a natural component of their ecology. Consequently, we hypothesize that human-caused wildfires could significantly affect soil invertebrate communities, with cascading impact on ecosystem processes provided by these communities, such as litter decomposition. We studied invertebrate diversity in the China Muerta National Reserve (38º S) eight years after a high-severity wildfire. The goal was to assess how community structure changed after different wildfire severities and their effect on litter decomposition. We used litterbags with two mesh sizes: 1 mm to prevent and 5 mm to allow invertebrate access. These were placed in areas affected by high- and low-severity wildfires, as well as an unburned control site. Significant declines in invertebrate richness, abundance, and diversity were seen in both high- and low-severity plots compared to the control plots. Control plots contained 2.2 and 1.9 times more taxonomic groups than high- and low-severity fire plots, respectively. Similar patterns were observed in abundance and diversity. Predators, omnivores, and phytophagous species were less abundant in burned plots. Although soil properties changed between burned and unburned plots, these changes were not significantly related to soil invertebrate composition and trophic groups. Wildfire significantly influenced the litter decomposition rate, with low-severity plots showing the slowest rates (0.041 year<sup>−1</sup>), followed by high-severity plots (0.086 year-<sup>1</sup>), and control plots (0.114 year<sup>−1</sup>). Decomposition rates decreased even further in litter bags inaccessible to invertebrates. Therefore, changes in the soil invertebrate community caused by fires contributed to the decline in litter decomposition, which could ultimately affect nutrient cycling within vulnerable forest ecosystems.</div></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"114 ","pages":"Article 151117"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146173723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PedobiologiaPub Date : 2026-03-01Epub Date: 2026-02-20DOI: 10.1016/j.pedobi.2026.151121
Stephen K. Kamesa , Esther N. Kioko , Yannan Chen , Jun Chen
{"title":"Forest conversion alters taxonomic and functional diversity of oribatid mite communities through shifts in environmental conditions in Taita Hills, Kenya","authors":"Stephen K. Kamesa , Esther N. Kioko , Yannan Chen , Jun Chen","doi":"10.1016/j.pedobi.2026.151121","DOIUrl":"10.1016/j.pedobi.2026.151121","url":null,"abstract":"<div><div>Understanding how soil biodiversity responds to forest conversion is essential for conserving ecosystem functioning and soil health in tropical montane regions. In tropical Africa, however, the effects of forest conversion on soil fauna, particularly oribatid mites, remain poorly explored. Oribatid mites are key decomposers that regulate nutrient cycling and serve as indicators of ecosystem integrity. This study investigated the impact of forest conversion on the taxonomic and functional diversity of oribatid mite communities across four ecosystem types: natural forest, cypress and eucalyptus plantations, and coffee farm—in the Taita Hills, Kenya. We analyzed abundance, species richness, diversity indices, community-weighted mean (CWM) traits, and their relationships with environmental variables. A total of 39,794 individuals representing 224 species were recorded. Abundance, richness, and the Shannon-Wiener diversity index were highest in the natural forest, intermediate in the exotic forest plantations, and lowest in the coffee farm. Functional richness (FRic) varied significantly among ecosystems (F₃,₁₆ = 34.02, p < 0.001). Community-weighted traits such as body colour and Pteromorph development also differed markedly, indicating trait filtering under altered conditions. Soil pH, litter thickness, and temperature were key predictors of community composition. Higher litter thickness and humidity supported greater diversity, whereas elevated temperature and soil pH reduced it. These findings demonstrate that land-use intensification modifies both taxonomic and functional aspects of soil biodiversity by altering critical environmental drivers. Conservation of litter-rich, micro-climatically stable forest habitats is therefore vital for maintaining soil biodiversity and sustaining ecosystem resilience in the face of continued forest conversion.</div></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"114 ","pages":"Article 151121"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147396890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PedobiologiaPub Date : 2026-03-01Epub Date: 2026-02-25DOI: 10.1016/j.pedobi.2026.151123
Dmitry D. Vinogradov , Marina G. Krivosheina , Ivan V. Sotnikov , Sergey M. Tsurikov , Andrey G. Zuev , Oksana L. Rozanova , Alexei V. Tiunov
{"title":"Diversity, abundance and stable isotope composition of winged Diptera (Insecta) emerging from temperate forest soil","authors":"Dmitry D. Vinogradov , Marina G. Krivosheina , Ivan V. Sotnikov , Sergey M. Tsurikov , Andrey G. Zuev , Oksana L. Rozanova , Alexei V. Tiunov","doi":"10.1016/j.pedobi.2026.151123","DOIUrl":"10.1016/j.pedobi.2026.151123","url":null,"abstract":"<div><div>Diptera (Insecta) play a significant role in forest ecosystems. With wingless larvae developing in soil and flying imagoes, they represent an important link between belowground detrital and aboveground grazing food webs. Despite this, trophic interactions of soil-dwelling Diptera remain poorly understood. In this study, we used emergence traps to follow seasonal changes in diversity, abundance and biomass of Diptera emerging from the soil in three temperate forest stands near Moscow, Russia and measured carbon and nitrogen stable isotope composition (δ<sup>13</sup>C and δ<sup>15</sup>N values) of emerging Diptera to assess their trophic positions. A greater taxonomic richness (88 species) was revealed by emergence traps compared to heat extraction of soil samples (41 species), highlighting the efficiency of traps in studying soil-associated Diptera. Stable isotope composition was measured for 65 species of winged flies and revealed a very broad range of δ<sup>15</sup>N values of individual samples (from −1.6‰ to 17.0‰) reflecting diverse trophic niches of Diptera. Notably, more than a half of the emerging dipteran biomass consisted of families with mean δ<sup>15</sup>N values exceeding typical δ<sup>15</sup>N values of soil predators (Mesostigmata, Chilopoda, and Araneae). The enrichment in <sup>15</sup>N can be attributed either to specific dietary sources such as fungi, carrion, and dung, and to an isotopic shift during metamorphosis. Thus, the upstream flux of emerging Diptera carries a specific isotopic signal, untypical of most soil saprophages and predators. This should be taken into account in the reconstructions of the trophic links between belowground and aboveground food webs.</div></div>","PeriodicalId":49711,"journal":{"name":"Pedobiologia","volume":"114 ","pages":"Article 151123"},"PeriodicalIF":2.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147396780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}