Revista Romana De Medicina De Laborator最新文献_第6页

Antibacterial Activity of Clove, Oregano, Thyme, Eucalyptus, and Tea Tree Essential Oils against Escherichia coli and Klebsiella pneumoniae strains 丁香、牛至、百里香、桉树和茶树精油对大肠杆菌和肺炎克雷伯菌的抑菌活性

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-07-01 DOI: 10.2478/rrlm-2022-0023

A. Băicuș, Flavio Cesare Mattuzzi, Ana-Maria Paraschiv, Rares-Sebastian Dinu, Maria Carina Dumitrescu, A. Marinescu, D. Ionescu, D. Dragoș

{"title":"Antibacterial Activity of Clove, Oregano, Thyme, Eucalyptus, and Tea Tree Essential Oils against Escherichia coli and Klebsiella pneumoniae strains","authors":"A. Băicuș, Flavio Cesare Mattuzzi, Ana-Maria Paraschiv, Rares-Sebastian Dinu, Maria Carina Dumitrescu, A. Marinescu, D. Ionescu, D. Dragoș","doi":"10.2478/rrlm-2022-0023","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0023","url":null,"abstract":"Abstract Background: In view of the high recurrence rate of urinary tract infections and the increasing number of germs resistant to multiple antibiotics, the aim of the present study was to evaluate the antibacterial properties of clove, oregano, thyme, eucalyptus, tea tree essential oils (EOs) against 32 isolates of Escherichia coli and 28 isolates of Klebsiella pneumoniae from patients with urinary tract infections (UTI). Methods: The agar disk diffusion method was used to assess the susceptibility of these isolates to essential oils and the minimal inhibitory concentration (MIC), and the minimal bactericidal concentration (MBC) were determined. Results: Our results suggest that volatile phenols (such as carvacrol in oregano EO, thymol in thyme EO, and eugenol in clove EO) are more efficacious as antibacterial than non-aromatic compounds (such as eucalyptol in eucalyptus EO and terpinene derivatives in tea tree EO). Conclusion : The oregano EO, followed by thyme appear to have the highest efficacy against Escherichia coli and Klebsiella pneumoniae isolates investigated.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"88 1","pages":"327 - 338"},"PeriodicalIF":0.5,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89052672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Approaching Risk Management in Medical Laboratories 接近医学实验室的风险管理

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-04-01 DOI: 10.2478/rrlm-2022-0017

R. E. David

{"title":"Approaching Risk Management in Medical Laboratories","authors":"R. E. David","doi":"10.2478/rrlm-2022-0017","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0017","url":null,"abstract":"Abstract Risk is one of the greatest challenges in a medical laboratory. Risk-based thinking is a concept that always preoccupies medical laboratory technicians. The objectives of this work were to bring forward the specialty standards recommendations for the implementation of risk management principles in medical laboratory, the accreditation requirement of ISO 15189:2012; to demonstrate that the understanding of the fundamental notions of the concept of risk, the effectively apply of the means of identifying, assessing and controlling risks, and the risk monitoring and handling through strategies of acceptance, elimination, transfer and mitigation of risks can ensure the continuous improvement processes. A model to approach to risk management in a medical laboratory establishes the inputs, the outputs, the techniques, and the activities carried out in each of the following sub-processes of the risk management process: risk management process planning, risk identification, risk analysis and evaluation, development of the risk response plan and risk monitoring, control and revision. The concepts of risk and risk management are applied accordingly to standards ISO 31000:2018 and ISO 22367:2020 of International Organization for Standardization (ISO). Risk management ensures that the fundamental requirements for healthy, proactive internal control of the medical laboratory are met.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"7 1","pages":"125 - 139"},"PeriodicalIF":0.5,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86199128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Snapshot of resistance and virulence features in ESCAPE strains frequently isolated from surgical wound infections in a Romanian hospital 罗马尼亚一家医院外科伤口感染中经常分离的ESCAPE菌株的耐药性和毒力特征快照

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-04-01 DOI: 10.2478/rrlm-2022-0014

Sajjad Mohsin I Rayyif, Wathek N. Alwan, H. Mohammed, I. Barbu, A. Holban, I. Gheorghe, O. Banu, O. Shalal, M. Chifiriuc, G. Mihăescu

{"title":"Snapshot of resistance and virulence features in ESCAPE strains frequently isolated from surgical wound infections in a Romanian hospital","authors":"Sajjad Mohsin I Rayyif, Wathek N. Alwan, H. Mohammed, I. Barbu, A. Holban, I. Gheorghe, O. Banu, O. Shalal, M. Chifiriuc, G. Mihăescu","doi":"10.2478/rrlm-2022-0014","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0014","url":null,"abstract":"Abstract Background: The aim of this study was to investigate the phenotypic features (adherence, biofilm formation, virulence, antibiotic susceptibility) and the genetic background of antibiotic resistance in nosocomial ESCAPE strains consecutively isolated from surgical wound infections in hospitalized patients. Methods: 86 bacterial strains consecutively isolated from various wound infections were analysed by their antibiotic resistance (antibiotic susceptibility testing and PCR for certain antibiotic resistance genes), virulence, biofilm formation and cellular adherence. Results: The bacterial isolates were identified as: Enterobacterales (n = 39) including Escherichia coli (n = 9), Klebsiella pneumoniae (n = 14) Proteus mirabilis (n = 7), followed by Staphylococcus aureus (n = 26) and Enterococcus faecalis (n = 20). Compared to other isolates, S. aureus strains exhibited the highest capacity to produce soluble virulence factors and to develop biofilms in vitro, with significant differences between methicillin resistant and methicillin susceptible isolates. Among enterobacterial isolates, K. pneumoniae strains expressed the highest capacity to develop biofilms. The assessment of bacterial adherence to HeLa cells revealed that all bacterial strains adhered to the cellular substrata, showing various adherence patterns. E. faecalis strains exhibited a low soluble virulence factors profile, a lower capacity to adhere to epithelial cells and to develop biofilms. Conclusions: The present study could contribute to the understanding of the pathology of infected wounds, depending on the etio-logical agents, providing data with positive impact on the therapeutic management of surgical wounds infections.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"17 1","pages":"215 - 226"},"PeriodicalIF":0.5,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84422226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of nitroglycerin combined with continuous regional arterial infusion on severe acute pancreatitis, triglyceride, inflammatory factors and prognosis 硝酸甘油联合持续局部动脉输注对重症急性胰腺炎、甘油三酯、炎症因子及预后的影响

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-04-01 DOI: 10.2478/rrlm-2022-0020

Bao-shan Mao, Hualiang Zhang, K. Wu

{"title":"Effects of nitroglycerin combined with continuous regional arterial infusion on severe acute pancreatitis, triglyceride, inflammatory factors and prognosis","authors":"Bao-shan Mao, Hualiang Zhang, K. Wu","doi":"10.2478/rrlm-2022-0020","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0020","url":null,"abstract":"Abstract Background: To evaluate the effects of nitroglycerin combined with continuous regional arterial infusion (CRAI) on severe acute pancreatitis (SAP), triglyceride (TG), inflammatory factors and prognosis. Methods: SAP patients were randomly divided into control and observation groups (n=169). The control group was treated with intravenous infusion of nitroglycerin once daily for 2 d, while the observation group was treated with nitroglycerin combined with CRAI for 7 d. Their hospitalization time, abdominal pain relief time, abdominal distension relief time and intestinal function recovery time were recorded. The levels of inflammatory factors, and TG were measured. Their liver and kidney functions, hemorheological indices, prognosis, and adverse reactions were evaluated. Results: The observation group had significantly shorter hospitalization time, abdominal pain relief time, abdominal distension relief time, and intestinal function recovery time than those of the control group (P<0.05). After treatment, the levels of interleukin-6, tumor necrosis factor-alpha, endothelin, thromboxane A2 and TG significantly decreased in contrast with those before treatment in both groups, especially in the observation group (P<0.05). The hemorheological indices were significantly improved after treatment compared with those before treatment in both groups, and the observation group had better indices (P<0.05). The Acute Physiology and Chronic Health Evaluation-II score declined significantly in both groups after treatment, and the observation group had a more obvious decrease (P<0.05). During follow-up, similar incidence rates of adverse reactions were observed for both groups (P>0.05). Conclusion: Nitroglycerin combined with CRAI is prominently effective for treating SAP, which can facilitate the health recovery and reduce the incidence of microcirculation disturbance-induced adverse events such as organ failure.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"5 1","pages":"191 - 198"},"PeriodicalIF":0.5,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87216418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proposal of a prediction model for prognosis of patients with acute myocardial infarction after percutaneous coronary intervention based on galectin-3 and soluble growth stimulating expressed gene 2 levels 基于半凝集素-3和可溶性生长刺激表达基因2水平的急性心肌梗死患者经皮冠状动脉介入治疗后预后预测模型的建立

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-04-01 DOI: 10.2478/rrlm-2022-0019

Fuxia Zhang, F. Yu, Songsong Song, Hongfei Yang, Liangfa Shao, J. Tong

{"title":"Proposal of a prediction model for prognosis of patients with acute myocardial infarction after percutaneous coronary intervention based on galectin-3 and soluble growth stimulating expressed gene 2 levels","authors":"Fuxia Zhang, F. Yu, Songsong Song, Hongfei Yang, Liangfa Shao, J. Tong","doi":"10.2478/rrlm-2022-0019","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0019","url":null,"abstract":"Abstract Background: To study the correlations of serum galectin-3 (Gal-3) and soluble growth stimulating expressed gene 2 (sST2) levels with prognosis of patients with acute myocardial infarction (AMI) after percutaneous coronary intervention (PCI). Methods: A total of 112 patients diagnosed from August 2015 to October 2017 were selected. They were followed up for 3 years. Based on major adverse cardiovascular events (MACEs) during follow-up, they were divided into MACE and non-MACE groups. Multivariate logistic regression analysis was performed to explore the independent risk factors for MACEs. A nomogram model was established using the factors and validated. The optimal cut-off values of Gal-3 and sST2 levels were determined by receiver operating characteristic curves. Kaplan-Meier method was used for survival analysis. Results: MACEs occurred in 78 patients during follow-up. Patients in the MACE group were more often hypertensive, had higher total cholesterol, uric acid, sST2 and Gal-3, and lower left ventricular ejection fraction (LVEF) (P<0.05). CK-MB, sST2, Gal-3 and LVEF were the independent risk factors for MACEs (P<0.05). The nomogram model established with these factors had high accuracy for predicting overall survival, and its concordance index (C-index) was 0.768 (95% confidence interval: 0.692-0.865). The prognosis of the patients with Gal-3 ≥12.57 μg/ mL and sST2 ≥18.56 ng/mL was poorer 3 years after PCI. Conclusions: The levels of serum Gal-3 and sST2 are the independent risk factors for MACEs in AMI patients following PCI, with high prognostic value.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"7 5","pages":"141 - 149"},"PeriodicalIF":0.5,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72401793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MMP-2 and MMP-9 gene polymorphisms and risk of head and neck carcinomas MMP-2和MMP-9基因多态性与头颈癌的风险

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-04-01 DOI: 10.2478/rrlm-2022-0010

S. Schipor, Adina Zamfir, D. Gheorghe, C. Picu, D. Manda

{"title":"MMP-2 and MMP-9 gene polymorphisms and risk of head and neck carcinomas","authors":"S. Schipor, Adina Zamfir, D. Gheorghe, C. Picu, D. Manda","doi":"10.2478/rrlm-2022-0010","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0010","url":null,"abstract":"Abstract Background: Head and neck carcinomas (HNC) account for a majority of ear, nose and throat tumours. They account for 6.3% of all incident malignancies and 6.2 % of all deaths from cancer in Romania in 2020, the fifth most common cancer in this Eastern Europe country. Aim of the study: The aim of our study was to investigate the association between two MMP-2 and MMP-9 promoter gene polymorphisms and head and neck cancer. Methods. We enrolled 142 subjects, 65 cancer patients, and 77 control subjects and tested them for MMP-2 -735 C/T and MMP-9 -1562 C/T polymorphisms by PCR-RFLP. Results. Comparison between cancer patients and controls demonstrated the presence of MMP-2 -735 C/T and MMP-9 -1562 C/T in head and neck malignant tumours, with OR = 2.206 (95% CI 1.058-4.599, P = 0.03) for MMP-2 and OR = 2.748 (95% C.I. 1.262-5.981, P=0.009) for MMP-9 gene polymorphism. This means that the presence of T allele could be a risk factor for head and neck cancer development. The analysis included a stratification of studied groups by age and gender. Conclusions. Both genotypes were associated with a significant risk for head and neck carcinomas in case of the presence of the T allele. MMP-2 -735 C/T (rs2285053) and MMP-9 -1562 C/T (rs3918242) gene polymorphism could be an important genetic marker for head and neck cancer susceptibility. This finding could be useful for genetic screening in head and neck carcinomas.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"45 1","pages":"163 - 171"},"PeriodicalIF":0.5,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76690849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Associations of serum expressions of miR-499 and sex determining region Y-box 6 with prognosis of acute myocardial infarction patients 急性心肌梗死患者血清miR-499及性别决定区Y-box 6表达与预后的关系

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-04-01 DOI: 10.2478/rrlm-2022-0011

Jing Mao, Bin Wang, Yingjia Li

{"title":"Associations of serum expressions of miR-499 and sex determining region Y-box 6 with prognosis of acute myocardial infarction patients","authors":"Jing Mao, Bin Wang, Yingjia Li","doi":"10.2478/rrlm-2022-0011","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0011","url":null,"abstract":"Abstract Background: To explore the associations of serum expressions of miR-499 and sex determining region Y-box 6 (SOX6) with major adverse cardiovascular and cerebrovascular events (MACCE) and prognosis of acute myocardial infarction (AMI) patients undergoing percutaneous coronary intervention (PCI). Methods: The clinical data of 132 patients diagnosed from February 2016 to October 2019 were collected. Serum miR-499 and SOX6 expressions were detected by RT-qPCR. Optimal cut-off values were determined using receiver operating characteristic curves, based on which patients were divided into low and high miR-499 expression groups, and high and low SOX6 expression groups. Survival curves were plotted using Kaplan-Meier method, and the independent risk factors for MACCE were explored by multivariate logistic regression analysis. A nomogram model was established based on the factors and validated using internal data. Results: AMI group had higher miR-499 expression and lower SOX6 expression than those of control group (P<0.05). After PCI, miR-499 expression decreased and SOX6 expression increased (P<0.05). Low miR-499 expression group had higher 3-year survival and MACCE-free rates than those of high miR-499 expression group (P<0.05). Low SOX6 expression group had lower 3-year survival and MACCE-free rates than those of high SOX6 expression group (P<0.05). AMI history, LVEF, CK-MB, miR-499 and SOX6 expressions were independent risk factors for MACCE (P<0.05). The nomogram model had high accuracy for predicting overall survival, with a concordance index of 0.742 (95%CI=0.684-0.845). Conclusions: AMI patients have increased serum expression of miR-499 and decreased expression of SOX6. High miR-499 expression is an independent risk factor for poor prognosis. The established nomogram model can be used to predict the risk of MACCE after PCI.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"20 1","pages":"151 - 161"},"PeriodicalIF":0.5,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73878057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Matrix-assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS) for subgingival bacteriome identification in a group of treated periodontitis patients: a case series 基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)用于牙周炎治疗患者牙龈下细菌组鉴定:一个病例系列

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-04-01 DOI: 10.2478/rrlm-2022-0012

Irina Lupșe, M. Flonta, A. Ciurea, I. Micu, A. Román, E. Páll, D. Popescu, A. Soancă

{"title":"Matrix-assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS) for subgingival bacteriome identification in a group of treated periodontitis patients: a case series","authors":"Irina Lupșe, M. Flonta, A. Ciurea, I. Micu, A. Román, E. Páll, D. Popescu, A. Soancă","doi":"10.2478/rrlm-2022-0012","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0012","url":null,"abstract":"Abstract Periodontitis is a chronic multifactorial polymicrobial infection, characterized by profound modifications of the composition and proportion of the subgingival microbiota. Microbiological laboratory tests are sometimes used in periodontal diagnosis and monitoring of treatment, but both conventional cultivation methods and molecular techniques have some major drawbacks. Therefore, other performant bacterial identification methods must be considered. The aim of the current study was to use Matrix-assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALTI-TOF MS) analysis in association with bacterial culture method to evaluate the modifications of the subgingival bacterial composition in periodontitis patients, before and after cause-related subgingival therapy. Subgingival plaque samples were collected from periodontal pockets before and after subgingival mechanical instrumentation and adjunctive local antimicrobial applications and were cultured in aerobic and anaerobic conditions. Microbial colonies were further assessed using MALDI-TOF-MS. A total of 36 bacterial strains were isolated from a group of 16 patients. All species from the orange complex were identified by MALDI-TOF MS. A marked reduction of detection frequency was observed in most bacterial strains, including the orange complex after cause-related periodontal treatment. The results of this study indicate that MALDI-TOF MS could be considered an accurate method for oral microbial identification and the cause-related periodontal treatment is useful for reducing the microbial burden.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"123 1","pages":"227 - 234"},"PeriodicalIF":0.5,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86015624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-inhibitory effects of the potent antioxidant C-phycocyanin from Plectonema sp. on the in vitro glycation reaction Plectonema sp.强抗氧化剂c -藻蓝蛋白对体外糖基化反应的非抑制作用

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-04-01 DOI: 10.2478/rrlm-2022-0018

A. Husain, Sultan Alouffi, Afreen Khanam, Rihab Akasha, Saif Khan, M. Khan, A. Farooqui, Saheem Ahmad

{"title":"Non-inhibitory effects of the potent antioxidant C-phycocyanin from Plectonema sp. on the in vitro glycation reaction","authors":"A. Husain, Sultan Alouffi, Afreen Khanam, Rihab Akasha, Saif Khan, M. Khan, A. Farooqui, Saheem Ahmad","doi":"10.2478/rrlm-2022-0018","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0018","url":null,"abstract":"Abstract When glucose and Amadori products are auto-oxidized, glycation occurs, resulting in the formation of early (Amadori) and late advanced glycation end products (AGEs), as well as free radicals. Glycation and an increase in free radical activity induce diabetic complications. Antioxidant and antiglycation compounds may aid in the prevention of oxidation and glycation. The goal of this study was to assess the antiglycation and antioxidant capacity of C-phycocyanin (C-PC) derived from Plectonema sp. The DPPH (1, 1-diphenyl-2-picrylhydrazyl), nitric oxide, hydroxyl radical scavenging activities and ferric ions reducing antioxidant power (FRAP) assays were used to assess antioxidant activity, while an in vitro bovine serum albumin-methyl glyoxal glycation (BSA-MG) model was used to assess glycation inhibitory potential. Glycation inhibition was measured using a variety of spectroscopic and biochemical parameters, including UV-visible & fluorescence spectroscopy, ketoamine, carbonyl and hydroxymethyl furfural content, as well as free lysine & free arginine estimations. In vitro, C-PC exhibited dose-dependent potent antioxidant activity, but lacked significant antiglycation potential. As a result, it is recommended that further studies be conducted to evaluate the antiglycation potential of C-PC.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"64 1","pages":"199 - 213"},"PeriodicalIF":0.5,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85396522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Crinum latifolium extract inhibits lipopolysaccharide-induced inflammation in human macrophages 荆叶提取物抑制脂多糖诱导的人巨噬细胞炎症

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-04-01 DOI: 10.2478/rrlm-2022-0015

S. Köktürk, Y. Çetinkol, S. Cırrık

{"title":"Crinum latifolium extract inhibits lipopolysaccharide-induced inflammation in human macrophages","authors":"S. Köktürk, Y. Çetinkol, S. Cırrık","doi":"10.2478/rrlm-2022-0015","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0015","url":null,"abstract":"Abstract Introduction: Crinum latifolium is a natural plant showing the anti-inflammatory effects. We aimed to evaluate the effects of Crinum latifolium extract on proinflammatory cytokine production and maturation of early and late endosomes in the lipopolysaccharide (LPS)-induced human macrophages at light microscopic and biochemical levels. Material and Methods: The effects of Crinum latifolium extract in human macrophages stimulated with LPS was evaluated to optic densities of the lysosome-associated membrane protein-1 (LAMP-1) and early endo-some antigen 1 (EEA1) by immunohistochemistry staining and to level of the proinflammatory cytokine interleukin (IL)-6 by enzyme-linked immunosorbent assay (ELISA). Results: Crinum latifolium group exhibited a significant decreased in the levels of IL-6 in the supernatant as compared to the LPS group for 2 and 72 hours (p<0.0001). Crinum latifolium group exhibited a significant increased in the optic density of the EEA1 and LAMP-1 in coverslips as compared to the control and lipopolysaccharide groups after 2 and 72 hours (p<0.0001). Conclusions: Crinum latifolium may a therapeutic plant in the inflammatory diseases, such as sepsis, through anti-inflammatory effects, such as decrease in production of the proinflammatory cytokine and increase in maturations of the early and late endosomes in macrophages.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"9 1","pages":"183 - 190"},"PeriodicalIF":0.5,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82345950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0