Revista Romana De Medicina De Laborator最新文献

{"title":"The potential value of some adipokines and cytokines as diagnostic biomarkers for prostate cancer","authors":"Pshtiwan Abdullah Yousif, Parween Abdulsamad Ismail","doi":"10.2478/rrlm-2023-0031","DOIUrl":"https://doi.org/10.2478/rrlm-2023-0031","url":null,"abstract":"Abstract Background : The role of Adipokines and proinflammatory cytokines is said to be crucial in the development of prostate cancer. Vaspin, Chemerin, Omentin, Interleukins IL-1β, interleukin-8 (IL8), Colony-stimulating factor (GM-CSF) and CC chemokine ligand 18 (CCL18) have all been proven to take part in tumor growth and progression. Aim of the study: The study aimed to explore circulating novel adipocytokines, such as serum of Vaspin, Chemerin and Omentin levels in patients with prostate cancer and to determine the level of selected proinflammatory cytokines (CCL18, IL-8, IL1, and GM-CSF). Methods : Three groups were included in the current study: Group (1) :32 patients with metastatic prostate cancer who received chemotherapy, Group (2): 30 untreated patients with nonmetastatic prostate cancer, and Group (3): 30 healthy controls. ELISA technique was used to assess serum levels of Vaspin, Chemerin, Omentin, CCL18, IL-8, IL1, and GM-CSF). Results : The Prostate cancer group exhibited higher serum levels of Vaspin, Chemerin, Omentin, CCL18, IL-8, IL1, and GM-CSF compared to the control group. Chemotherapy-treated patients exhibited significantly increased levels of the pro-inflammatory cytokines (IL-8) and Adipokines (Vaspin and Omentin) and decreased levels of the pro-inflammatory cytokines (IL-1) and Adipokines (Chemerin). The correlation analysis showed a significant positive correlation of serum Chemerin with Vaspin (r = 0.957, p-value<0.0001), IL-8 (r = 0.9475, p-value < 0.0001) and IL-1β (r = 0.7771, p-value < 0.0029). Omentin and GS-CSF levels showed a non-significant positive correlation with Chemerin level (r = 0.1259; p = 0.6967).) and (r = 0.4247; p = 0.1688), respectively. While significant negative correlation was found between (Chemerin) with CCL-18 (r = –0.7916, p = 0.0022), serum Vaspin was significantly and negatively correlated with the levels of CCL-18 (r = –0.9349, p < 0.0001), whereas there was a significant positive correlation between Vaspin level with IL-8 (R=0.9995, p <0.0001); IL-1β(r = 0.561, p = 0.0057). The data demonstrated that Vaspin was positively and non-significantly correlated with the level of GS-CSF (r = 0.1437, p =0.656); serum Omentin was significantly and negatively correlated with the levels of GS-CSF (r = –0.8447, p = 0.0005), and CCL-18 (r= –0.7058, p = 0.0103), whereas there was a non-significant positive correlation between Omentin level with IL-8 (r = 0.4364, p = 0.1561). The data demonstrated that Omentin was negatively and non-significantly correlated with the level of IL-1β (r= –0.5366, p =0.0786). Conclusions : This study indicated increased levels of serum Vaspin, Chemerin, Omentin, Interleukins IL-1β, interleukin-8 (IL8), Colony-stimulating factor (GM-CSF) and CC chemokine ligand 18 (CCL18) in patients with Prostate cancer. These findings suggest that the cytokines, and adipokines, whose levels were elevated in the chemotherapy-treated patients may be involved in the pathophysiology of pros","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136127581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analyzing serum tryptophan metabolites in patients with gestational diabetes mellitus","authors":"Muammer Özdemir, Sedat Abusoglu, Suleyman Baldane, Cem Onur Kıraç, Ali Unlu, Duygu Eryavuz Onmaz, Murat Çelik, Gulsum Abusoglu","doi":"10.2478/rrlm-2023-0027","DOIUrl":"https://doi.org/10.2478/rrlm-2023-0027","url":null,"abstract":"Abstract Introduction : Although pregnancy is a physiological condition, the secretion of diabetogenic hormones such as growth hormone, corticotropin-releasing hormone, placental lactogen hormone, prolactin and progesterone from the placenta could lead to insulin resistance (IR). In Metabolic Syndrome, obesity and Type1&amp;2 diabetes, a shift in the kynurenine pathway (KP) towards IDO activation is observed. The activation of the IDO leads to the activation of the Aryl hydrocarbon receptor (AhR) and Interleukin-6 (IL-6) also, which may also induce some effects like insulin resistance, β-cell disfunction and increased gluconeogenesis. We hypothesized that the overactivation of IDO and some KP enzymes would be observed in GDM patients, in a similar manner to metabolic syndrome, prediabetes, and diabetes patients. Methods : 50 patients and 50 controls, who applied to the Endocrinology outpatient clinic of Selcuk University Faculty of Medicine were included. Serum triptophan metabolite levels were measured with liquid chromatography tandem mass spectrometry. Results : Tryptophan and KYNA values was found to be lower in the patient group diagnosed with GDM (p<0.001 and p<0.001, respectively). The levels of KYN, 3-OH AA, 3-OH-KYN and KTR were significantly higher in the patient group compared to the control group (p=0.008, p<0.001, p=0.05 and p<0.001, respectively). Conclusions : Understanding the changes that occur in this pathway in GDM patients may provide insight into the development of the disease. Also these tests could be used as supplementary tests in gestational diabetes, which could assist in diagnosis and patient follow-up.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136160529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlations of special AT-rich sequence binding protein 2 and chitinase-3-like protein-1 with sensitivity to paclitaxel chemotherapy for gastric cancer","authors":"Hesi Zhuang, Limin Huang, Zhengqi Yang","doi":"10.2478/rrlm-2023-0030","DOIUrl":"https://doi.org/10.2478/rrlm-2023-0030","url":null,"abstract":"Abstract Background : Our objective was to examine the associations between special AT-rich sequence binding protein 2 (SATB2) and chitinase-3-like protein-1 (CHI3L1) and the responsiveness to paclitaxel treatment in individuals with gastric cancer. Methods : From March 2018 to October 2020, our hospital collected gastric cancer samples along with adjacent gastric mucosal tissues located more than 5 cm away from the cancerous margin. These samples were obtained from 90 patients who underwent chemotherapy regimens containing paclitaxel. To assess the rates of positive expression of CHI3L1 and SATB2 in gastric cancer and adjacent tissues, the immunohistochemical streptavidin-peroxidase (SP) technique was utilized. Results : The positive expression rate of CHI3L1 was higher in gastric cancer tissues compared to adjacent tissues, while the positive expression rate of SATB2 was lower (P<0.05). Risk factors that influenced the positive expression of CHI3L1 in gastric cancer tissues included the level of differentiation, tumor-node-metastasis (TNM) stage, and the presence of lymph node metastasis (OR>1, P<0.05). Additionally, the positive expression of SATB2 was also affected by TNM stage and lymph node metastasis, which were identified as risk factors (OR>1, P<0.05). In gastric cancer tissues, there was a negative correlation observed between the expressions of CHI3L1 and SATB2 (r<0, P<0.05). According to the analysis results of Kendall’s tau-b (K), it was found that the presence of CHI3L1 had an inverse relationship with the responsiveness to paclitaxel-based chemotherapy in gastric cancer (r=-0.498, P=0.000), while SATB2 exhibited a positive correlation with the sensitivity (r=0.513, P=0.000). During the 3-year follow-up after chemotherapy, the survival rate was 55.55% (50/90). Conclusions : The findings indicate a strong correlation between SATB2 and CHI3L1 with the TNM stage, lymph node metastasis, response to paclitaxel-based chemotherapy, and the overall survival rate of individuals.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136127583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum cytokine and chemokine profiles of patients with confirmed bacterial and viral meningitis","authors":"Ramona Caragheorgheopol, Cătălin Țucureanu, Veronica Lazăr, Iuliana Caraș","doi":"10.2478/rrlm-2023-0023","DOIUrl":"https://doi.org/10.2478/rrlm-2023-0023","url":null,"abstract":"Abstract Introduction : Cerebrospinal fluid (CSF) cytokines and chemokines have been reported by several studies as useful markers to discriminate bacterial and viral meningitis (BM and VM). This study aimed to investigate if serum cytokine and chemokine profiles could also differentiate BM from VM, thus circumventing the need for an invasive lumbar puncture. Methods : Serum cytokines and chemokines were measured in 153 samples from patients with BM (n=58), VM (n=69), and controls (C, n=26) using multiplex assays. Cytokine and chemokine concentrations were compared among groups, correlation analyses were performed, and BM and VM cases classification based on cytokine and chemokine patterns was tested using a Machine Learning algorithm. Results : IL-8, IL-1β, IL-6, IL-10, TNF-α, MCP-1, and ENA-78 showed a pronounced increase in the BM group compared to C (P<0.01). Comparison of cytokines and chemokines in BM vs. VM showed significantly higher levels of MCP-1, IL-8, IL-1β, IL-6 and IL-10 (P<0.01). Serum cytokine and chemokine concentrations were highly correlated in BM, being strongest for: MCP-1/IL-8, MCP-1/IL-1β, and IL-8/IL-1β (r=0.83; r=0.72; r=0.78, respectively). In VM, cytokine and chemokine correlations were weaker. The best predictors in the cytokine and chemokine pattern identified with a Random Forest algorithm for classifying BM vs VM were IL-8 and IL-10, and IL-6, but the specificity and sensitivity were low (85% and 69%, respectively). Conclusion : Our results suggest significant changes in serum IL-6, IL-8, IL-10, and IL-1β in BM, but these mediators may have limited value in differentiating BM from VM.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136160749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Updated insights into predictive biomarkers for response to cardiac resynchronization therapy - A literature review","authors":"Krisztina Pal, Ionela M. Cotoi, Georgiana G. Vlad, Silvia Lupu","doi":"10.2478/rrlm-2023-0025","DOIUrl":"https://doi.org/10.2478/rrlm-2023-0025","url":null,"abstract":"Abstract Cardiac resynchronization therapy (CRT) was shown to greatly improve outcomes in specific patients with heart failure, low ejection fraction and wide QRS complex; however post-therapeutic response is heterogeneous and currently difficult to predict. There is an increasing interest in identifying humoral biomarkers which could help stratify prognosis, and better identify responders. The aim of this review was to provide an overview of recent data regarding the predictive value of biomarkers for evaluating response to CRT. A definitive conclusion cannot currently be drawn due to disparate results, varying methodologies, relatively small-scale studies and lack of consensus in defining CRT response. More extensive comparable research is paramount to facilitate progress in this field.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136160753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations of levels of high-molecular-weight adiponectin, secreted frizzled-related protein 5 and vascular endothelial growth factor-165 with diabetic retinopathy","authors":"Yanfang Fu, Shizhen Ye, Jianjun Peng","doi":"10.2478/rrlm-2023-0028","DOIUrl":"https://doi.org/10.2478/rrlm-2023-0028","url":null,"abstract":"Abstract Background : The pathogenesis of diabetic retinopathy (DR) remains unclear. The aim of the study was to explore the associations of DR with the levels of high-molecular-weight adiponectin (HMW-ADP), secreted frizzled-related protein 5 (SFRP-5) as well as vascular endothelial growth factor-165 (VEGF165). Methods : Based on the diagnostic criteria for DR, non-DR (NDR), proliferative DR (PDR) plus non-proliferative DR (NPDR) groups were set up for type 2 diabetes mellitus (T2DM) patients (n=180) treated from January 2020 to March 2021. The control group consisted of another 60 healthy subjects undergoing physical examinations. Their clinical data were compared. Receiver operating characteristic curves were plotted to assess the predictive values of HMW-ADP, VEGF165 and SFRP-5 for DR. The predictive efficiency of the established nomogram model was assessed. Results : The differences in age, fasting plasma glucose (FPG), T2DM duration, triglyceride (TG), hypertension history, high-density lipoprotein cholesterol (HDL-C), glycosylated haemoglobin (HbA1c), serum creatinine (Scr), homeostasis model assessment of insulin resistance (HOMA-IR), urine acid (UA), SFRP-5 HMW-ADP, and VEGF165 were significant between control and DR groups (P<0.05). HMW-ADP, VEGF165 and SFRP-5 had predictive values for DR (AUC>0.7), and the predictive efficiency of their combination was highest. The duration of T2DM, Scr, UA and VEGF165 were independent risk factors for DR, while HMW-ADP and SFRP-5 were protective factors (P<0.05). Preferable discrimination and accuracy together with clinical applicability were obtained for the nomogram prediction model. Conclusions : The three indicators as a whole have a high predictive value for DR, as potential indicators for the clinical screening of high-risk groups.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136127580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlations of contrast-enhanced ultrasound parameters with free thyroxine, total thyroxine, thyroid peroxidase antibody and thyroglobulin antibody in patients with thyroid nodules","authors":"Jin Zhang, Zijun Wang, Peijie Guo","doi":"10.2478/rrlm-2023-0024","DOIUrl":"https://doi.org/10.2478/rrlm-2023-0024","url":null,"abstract":"Abstract Background : We aimed to study the contrast-enhanced ultrasound features and parameters of patients with benign and malignant thyroid nodules. Methods : A total of 154 patients diagnosed with thyroid nodules from January 2021 to December 2022 were selected as the subjects. They were divided into a benign nodule group (n=86) and a malignant nodule group (n=68). All patients were examined by contrast-enhanced ultrasound to analyze the features and the differences in parameters such as time to peak (Tp), mean transit time of contrast agent (MTT), peak intensity (PI), and area under curve (AUC). Results : Using surgical histopathological results as the gold standard, the sensitivity, specificity, and accuracy of contrast-enhanced ultrasound for diagnosing thyroid nodules were 88.24% (60/68), 83.72% (72/86), and 85.71% (132/154), respectively. Compared to the benign nodule group, the levels of FT4, and TT4 significantly decreased, whereas those of TPOAb and TGAb significantly increased in the malignant nodule group (P<0.05). MTT, PI, and AUC were positively correlated with FT4 and TT4 expressions but negatively correlated with TPOAb and TGAb expressions (P<0.05). The diagnosis of thyroid nodules combined with contrast-enhanced ultrasound parameters had an AUC of 0.950, sensitivity of 93.42%, specificity of 87.90%, and 95% CI of 0.832-0.987, and the diagnostic efficiency exceeded those of diagnosis with single indicators (P<0.05). Conclusions : The contrast-enhanced ultrasound parameters MTT, PI, and AUC decreased in malignant thyroid nodules, being closely correlated with the thyroid function status. The images in combination with parameters of contrast-enhanced ultrasonography can be used to increase the accuracy of diagnosing benign and malignant thyroid nodules.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136153745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and clinical aspects of HNSCC in the Republic of Moldova","authors":"Valentina Stratan, Valeri Țuțuianu, Victor Sîtnic, Cristina Popa, Veronica Balan, Adrian Clipca, Ina Sclifos, Anastasia Monastirscaia, Victoria Trohin, Mariana Sprincean","doi":"10.2478/rrlm-2023-0026","DOIUrl":"https://doi.org/10.2478/rrlm-2023-0026","url":null,"abstract":"Abstract Introduction : Complex molecular characterization and integrated approaches in the basic research of HNSCC provide new insights into the understanding and treatment of these tumors. Mutations in the TP53 gene, HPV infection, aberrant DNA methylation are just a few factors that have a direct link with the clinical and psychological condition of patients with this type of cancer. In the Republic of Moldova, these aspects are insufficiently studied. Methods : The study included 128 patients with HNSCC from whom the following samples were collected: fresh tumor tissue, NAT, blood, and saliva. All samples, except saliva, were tested for 3 mutations in the TP53 gene, while DNA isolated from tumor tissue was also tested for global DNA methylation assessment. HPV genotypes were tested from saliva. HPV positive samples were retested from tumor tissue. Results : Of the total analyzed samples for TP53 pathogenic variants, in 30 (23.44%) samples there were detected one or two mutations, and in 9 samples (7.03%) – it was detected the presence of two mutations simultaneously. HPV infection was detected in 17 samples (13.28%). Regarding global DNA methylation, in patients with a high degree of exposure to stress, a 44% lower level was observed (median 13.5 ng/ml) compared to those with moderate and low exposure (median 20.5 ng/ml ). Conclusion : The most frequent mutation identified in the TP53 gene was the 524G&gt;A substitution and the frequency of high-risk HPV infection in HNSCC patients from Moldova was 13.28%. The high degree of stress exposure showed a lower level of global methylation.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136160757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic patterns in glomerular research, a laser capture microdissection and liquid chromatography-tandem mass spectrometry approach","authors":"Andrada A. Bărar, Ioana E. Pralea, Ioana Berindan-Neagoe, Radu Pirlog, Andreea Nutu, Yuriy Maslyennikov, Alina R. Potra, Cristina A. Iuga, Ina M. Kacso","doi":"10.2478/rrlm-2023-0029","DOIUrl":"https://doi.org/10.2478/rrlm-2023-0029","url":null,"abstract":"Abstract Introduction : Molecular techniques have the potential to shed light on glomerular diseases that conventional renal pathology may be unable to reveal. The aim of this study was to investigate whether proteomic patterns of glomeruli obtained from kidney biopsies can differentiate between minimal change disease (MCD), focal segmental glomerulosclerosis (FSGS) and control groups (CTR). Methods : 18 formalin-fixed, paraffin-embedded (FFPE) renal biopsies comprising three groups of samples (CTR=3, MCD=6, FSGS=9) were subjected to label-free quantitative mass spectrometry. Glomeruli were excised from FFPE renal biopsies by laser capture microdissection (LCM) and, to increase both yield and protein identifications, single-pot solid-phase-enhanced sample preparation (SP3) digest method was applied. The samples were analyzed by mass spectrometry based shotgun proteomics. Results : The proteome profiling resulted in the identification of a total of 723 proteins. Multivariate analysis provided several proteins important in the separation of the three groups. Pattern Hunter analysis revealed moderate and high correlation of proteins against CTR-FSGS-MCD or CTR-MCD-FSGS patterns. The most significant pathways involved were associated with nephrin family and cytoskeleton interactions, as well as laminin/extracellular matrix related proteins. Univariate analysis revealed 58 significant different proteins among the three groups. Signaling pathways of these proteins were also associated with nephrin family interactions and cytoskeleton organization. Conclusions : This study demonstrates that mass spectrometry-based shotgun proteomic analysis of LCM glomeruli yields reproducible and quantitative data capable of discriminating between different disease conditions. Differentially expressed proteins provide insights into pathogenesis of glomerular disease.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136160533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical application value of Abbott Alinity analyzer in syphilis-specific antibody testing","authors":"Shu-Guang Hou, Congcong Cheng, Shuhong Yan","doi":"10.2478/rrlm-2023-0017","DOIUrl":"https://doi.org/10.2478/rrlm-2023-0017","url":null,"abstract":"Abstract Background: We aimed to investigate the clinical application value of Abbott Alinity analyzer in syphilis-specific antibody testing. Methods: A total of 100 patients admitted from June 2021 to June 2022 for early syphilis diagnosis were selected and subjected to Treponema pallidum (TP) antibody testing by chemiluminescent microparticle immunoassay (CMIA) using Abbott Alinity analyzer. With TP particle agglutination (TPPA) retesting as the gold standard for syphilis diagnosis, the signal-to-cutoff (S/CO) ratio of the TP antibody testing was plotted into the receiver operating characteristic curve to determine the diagnostic value of CMIA and CLIA in detecting positive TP antibody and to identify the optimal cutoff point. Results: In the case of S/CO ratio ≥7.00, the patients with positive CMIA were diagnosed with positive findings after TPPA confirmation. With the S/CO ratio of 1.00-4.99, the coincidence rate of CLIA with positive TPPA was 81.82% (45/55), and all patients with positive CLIA had positive results confirmed by TPPA test when the S/CO ratio was >5.00. When the optimal cutoff value of S/CO ratio for TP was determined as 6.98 by CMIA, the sensitivity, specificity, and maximum area under the curve (AUC) were 94%, 88% and 0.91, respectively. At the optimal cutoff value (S/CO ratio: 4.56) determined by CLIA, the sensitivity was 84%, the specificity was 80%, and the maximum AUC was 0.84. Conclusions: In the case of S/CO ratio ≥7.00, both methods have high sensitivity and specificity, which can directly give positive reports and shorten the sample turnaround time.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"135 1","pages":"185 - 190"},"PeriodicalIF":0.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86572589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}