Revista Romana De Medicina De Laborator最新文献_第7页

Expressions and clinical significance of interleukin-1β and cyclooxygenase-2 in serum and synovia of patients with gouty arthritis 痛风性关节炎患者血清和滑膜中白细胞介素-1β和环氧合酶-2的表达及临床意义

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-01-01 DOI: 10.2478/rrlm-2021-0037

Gang Chen, Chenghao Xiang, Lin-xia Zou, Li Xu, Zhi-Ming Cheng, Yong Liu

{"title":"Expressions and clinical significance of interleukin-1β and cyclooxygenase-2 in serum and synovia of patients with gouty arthritis","authors":"Gang Chen, Chenghao Xiang, Lin-xia Zou, Li Xu, Zhi-Ming Cheng, Yong Liu","doi":"10.2478/rrlm-2021-0037","DOIUrl":"https://doi.org/10.2478/rrlm-2021-0037","url":null,"abstract":"Abstract Background: To explore the expressions and clinical significance of interleukin-1β (IL-1β) and cyclooxygenase-2 (COX-2) in serum and synovia of patients with gouty arthritis (GA). Materials and Methods: A total of 110 GA patients hospitalized from January 2016 to September 2019 were selected as observation group, with 55 at remission stage (observation group I) and 55 at acute attack stage (observation group II). Another 55 healthy volunteers were selected as control group. The correlations of IL-1β and COX-2 with uric acid (UA) were explored by Spearman’s analysis. IL-1β and COX-2 expression levels were compared at different time points after treatment. Results: IL-1β, COX-2 and UA levels of patients were significantly higher in observation group I and II than those in the control group, and higher in the observation group II than those in observation group I. IL-1β and COX-2 were positively correlated with UA (P<0.05). ROC curve analysis showed that the optimal cut-off values of IL-1β and COX-2 for predicting GA were 18.23 and 9.12 pg/mL, and AUC values were 0.702 and 0.714 (P<0.001), respectively. On admission and after treatment for 3, 7, and 10 days, IL-1β and COX-2 levels of observation group II significantly exceeded those of observation group I (P<0.05). Compared with the efficacy group, after treatment for 14 days, IL-1β and COX-2 levels in serum and synovia significantly increased in the non-efficacy group (P<0.05). Conclusion: IL-1β and COX-2 levels in serum and synovia of GA patients are evidently higher than those in the control group, which rise with the aggravation of disease.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"1 1","pages":"41 - 48"},"PeriodicalIF":0.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74480496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of neutrophil-to-lymphocyte ratio as an outcome predictor factor in acute appendicitis: a single medical center’s experience 中性粒细胞与淋巴细胞比率作为急性阑尾炎预后预测因子的作用:单个医疗中心的经验

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-01-01 DOI: 10.2478/rrlm-2022-0002

B. Grigorescu, B. Suciu, Irina Săplăcan, R. Fodor, S. Voidăzan, Florentina C. Scarlat, C. Molnar, I. Halmaciu

{"title":"The role of neutrophil-to-lymphocyte ratio as an outcome predictor factor in acute appendicitis: a single medical center’s experience","authors":"B. Grigorescu, B. Suciu, Irina Săplăcan, R. Fodor, S. Voidăzan, Florentina C. Scarlat, C. Molnar, I. Halmaciu","doi":"10.2478/rrlm-2022-0002","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0002","url":null,"abstract":"Abstract Introduction: Acute abdominal pain persists in defying modern diagnostic procedures. Inflammatory response variables in urine and blood tests can help rule out other disorders and support a clinical diagnosis of acute appendicitis (AA), but there is no specific blood test that can diagnose AA. In order to address this, our study sought to establish the value of (NLR) as a prognostic and severity predictor in patients with AA. Material and method: We conducted an observational retrospective study of 356 patients who had undergone surgery for AA, assessing NLR as an outcome metric. The NLR was estimated using the differential WBC (white blood count) reported on admission; the data obtained were then recorded in a database and statistically analyzed. Results: The NLR medians correlated (p<0.001) in uncomplicated AA, AA with localized peritonitis, and AA with generalized peritonitis (Kruskal-Wallis test). The ROC curve identified 81.4 % sensitivity, 62.5 % specificity, and a 73 % AUC in localized peritonitis. The NLR value was associated with generalized peritonitis with a sensitivity of 63.7 %, specificity of 64.3 %, and AUC of 68.2%. The NLR-patient death association had 100% sensitivity, 80.3% specificity, and 93.5 AUC. Conclusion: NLR determination in individuals with acute appendicitis may be useful in predicting complications. NLR values greater than 7.86 are usually related with generalized peritonitis, while values larger than 12.9 may predict an increased risk of patient death. Calculating NLR from a complete blood count is a straightforward and cost-effective method of analyzing complicated AA in resource-constrained settings.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"36 1","pages":"63 - 70"},"PeriodicalIF":0.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88446159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of lactoferrin on osteogenic differentiation and related gene expressions of osteoblast precursor cells MC3T3-E1 under mechanical strain 乳铁蛋白对机械应变下成骨前体细胞MC3T3-E1成骨分化及相关基因表达的影响

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-01-01 DOI: 10.2478/rrlm-2022-0003

Jianfeng Sun, Xiaoting Zhu, Mengke Ge, Chenchen Zhou, Yuyu Li

{"title":"Effects of lactoferrin on osteogenic differentiation and related gene expressions of osteoblast precursor cells MC3T3-E1 under mechanical strain","authors":"Jianfeng Sun, Xiaoting Zhu, Mengke Ge, Chenchen Zhou, Yuyu Li","doi":"10.2478/rrlm-2022-0003","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0003","url":null,"abstract":"Abstract We aimed to evaluate the effects of lactoferrin (LF) on the osteogenic differentiation and related gene expressions of mouse embryonic osteoblast precursor cells MC3T3-E1 under mechanical strain. MC3T3-E1 cells were randomly divided into control, strain loading, LF and strain loading + LF groups. Alkaline phosphatase (ALP) activity was measured. Cytoskeletal morphology was measured by rhodamine-phalloidin staining. Formation of mineralized nodules was observed by alizarin red staining. The expressions of differentiation-related genes type I collagen (COL-1), interleukin-6 (IL-6) and osteocalcin (OCN) were determined by RT-PCR, and those of p-Runx2 and p-ERK1/2 proteins were detected by Western blotting. The number of ALP positive cells and expressions of OCN, COL-1 and IL-6 were significantly elevated (P<0.05). The optical density of strain loading + LF group was higher than those in strain loading and LF groups after incubation for 4 and 7 days (P<0.05). The cell volume and extension range were elevated in strain loading + LF group compared with those in strain loading group. The amount of mineralized nodules in strain loading + LF group was significantly higher than those in strain loading and LF groups, while it was slightly higher in LF group than that in strain loading group. The expressions of p-ERK1/2 and p-Runx2 in strain loading + LF group exceeded those in strain loading and LF groups (P<0.05). The synergistic action of LF and mechanical strain can effectively promote the proliferation, differentiation and mineralization of osteoblasts, probably being associated with the ERK1/2 signaling pathway.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"15 1","pages":"19 - 30"},"PeriodicalIF":0.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75974197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An insight into the fecal microbiota composition in Romanian patients with ankylosing spondylitis using high-throughput 16S rRNA gene amplicon sequencing 利用高通量16S rRNA基因扩增子测序深入了解罗马尼亚强直性脊柱炎患者的粪便微生物群组成

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-01-01 DOI: 10.2478/rrlm-2022-0004

M. Oprea, D. Cristea, S. Dinu, S. Ciontea, V. Bojinca, D. Predețeanu, A. Balanescu, C. Usein

{"title":"An insight into the fecal microbiota composition in Romanian patients with ankylosing spondylitis using high-throughput 16S rRNA gene amplicon sequencing","authors":"M. Oprea, D. Cristea, S. Dinu, S. Ciontea, V. Bojinca, D. Predețeanu, A. Balanescu, C. Usein","doi":"10.2478/rrlm-2022-0004","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0004","url":null,"abstract":"Abstract Introduction. Application of next-generation sequencing technology generated a massive amount of information on the gut microbiome composition used to understand its role in the healthy state and in various diseases. We aimed to provide information on the gut microbiota composition of Romanian subjects diagnosed with ankylosing spondylitis, an immune-mediated arthropathy linked to a genetic predisposition and gut dysbiosis. Methods. Stool samples collected from 25 patients with ankylosing spondylitis and 16 healthy controls were investigated using high-throughput DNA sequencing of 16S rRNA amplicons from seven different hypervariable regions and Ion Torrent PGM instrument. Microbial composition of metagenomic data was analyzed with QIIME software and differential abundance analysis of taxa encompassed linear discriminant analysis effect size (LEfSe). Results. Overall, 14 phyla, 114 families, 114 genera, and 275 species were identified across the 41 samples, the aggregated data revealing as most abundant the phyla Bacteroidetes, Firmicutes, and Proteobacteria, the families Bacteroidaceae, Prevotellaceae, and Ruminococcaceae, the genera Bacteroides, Prevotella, and Faecalibacterium, and Prevotella copri species. Using various cutoffs for abundance and prevalence, core taxonomic members were identified which in general were shared between the patients and controls. However, evidence was gained that the diversity in the microbiomes from the former cohort was lower than for controls and that certain taxa had significantly different abundance between the two groups. Conclusion. This study allowed an informative high-throughput 16S rRNA profiling of the gut microbiota needed to identify microbiome signatures of risk in the autochthonous population with AS.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"49 1","pages":"49 - 61"},"PeriodicalIF":0.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78218939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of iron replacement therapy efficiency using a complex panel of biomarkers 利用生物标志物的复杂面板评估铁替代疗法的有效性

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-01-01 DOI: 10.2478/rrlm-2022-0005

S. Pál, Z. Faust, B. Réger, A. Miseta, M. Varjú-Solymár, E. Nemes-Nagy, M. Tilinca, H. Alizadeh

引用次数: 0

Association of C5a/C5aR pathway to activate ERK1/2 and p38 MAPK in acute kidney injury – a mouse model 急性肾损伤小鼠模型中C5a/C5aR通路激活ERK1/2和p38 MAPK的关联

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-01-01 DOI: 10.2478/rrlm-2022-0001

Zeying Zhang

{"title":"Association of C5a/C5aR pathway to activate ERK1/2 and p38 MAPK in acute kidney injury – a mouse model","authors":"Zeying Zhang","doi":"10.2478/rrlm-2022-0001","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0001","url":null,"abstract":"Abstract Acute inflammation is accompanied by complement system activation and inflammatory cell accumulation. Acute kidney injury (AKI) is one of the common clinical symptoms, it is not clear whether complement system-mediated signaling pathway is involved. This study demonstrated that the expressions of complement C5a and C5a receptor (C5aR) protein in a mouse model with glycerol induced AKI were significantly increased, and the expression of inflammatory cytokines, such as IL-1β, IL-6 and TNF-α, were significantly higher than those in the blank control group. While C5aR antagonist (C5aRa) was added, western analyses for C5a and C5aR were reduced, meanwhile, qPCR and ELISA data showed that inflammatory cytokines also decreased significantly. In addition, preliminarily explored, the Mitogen-activated protein kinases (MAPKs) can be activated by the C5a/C5aR pathway in an AKI mouse model which showed that the C5a/C5aR pathway in a mouse model group activated ERK1/2 and p38, and the protein expression decreased when C5aRa was added. In conclusion, these results indicate that the C5a/C5aR pathway promotes renal pathogenesis by activating ERK1/2 and p38 expression and then affects the disease process, which has certain guiding significance for the subsequent clinical trial.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"4 1","pages":"31 - 40"},"PeriodicalIF":0.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87395922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Values of PCT and STREM-1 combined with clinical pulmonary infection score for the prognostic evaluation of elderly patients with ventilator-associated pneumonia PCT和STREM-1结合临床肺部感染评分对老年呼吸机相关性肺炎患者预后评价的价值

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-01-01 DOI: 10.2478/rrlm-2022-0006

Jianjun Wang, Xiaoyan Zhu, Xiaoling Wang, Xi Li

{"title":"Values of PCT and STREM-1 combined with clinical pulmonary infection score for the prognostic evaluation of elderly patients with ventilator-associated pneumonia","authors":"Jianjun Wang, Xiaoyan Zhu, Xiaoling Wang, Xi Li","doi":"10.2478/rrlm-2022-0006","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0006","url":null,"abstract":"Abstract Objective: To explore the values of PCT and STREM-1 combined with clinical pulmonary infection score (CPIS) for evaluating ventilator-associated pneumonia (VAP) in elderly patients. Methods: A total of 120 eligible patients were selected as VAP group, and 60 other patients without VAP were selected as control group. According to APACHE II scores, VAP group was divided into low-, medium-, and high-risk groups. The correlations of PCT, STREM-1, and CPIS with APACHE II score were analyzed. The values of PCT and STREM-1 combined with CPIS for evaluating prognosis were analyzed using ROC curves. Results: In the VAP group, serum PCT and STREM-1 levels and CPIS after mechanical ventilation significantly exceeded those of the control group and before mechanical ventilation (P<0.05). These values were higher in the high-risk group than in medium- and low-risk groups (P<0.05). They were positively correlated with APACHE II score (P<0.001). These values of the death group were significantly higher than those of the survival group 1, 3, 5, and 7 d after treatment (P<0.05). AUC of PCT and STREM-1 combined with CPIS for assessing prognosis was 0.892 (P<0.001). Conclusions: Serum PCT and STREM-1 levels and CPIS significantly increase in elderly patients with VAP, which rise with aggravation. Their combination has higher values for prognostic evaluation.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"4 1","pages":"71 - 79"},"PeriodicalIF":0.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79604158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Resveratrol on Sphingosine-1 and Oxidative/ Nitrosative Stress in an Experimental Heart Ischemia Reperfusion Model 白藜芦醇对实验性心脏缺血再灌注模型鞘氨醇-1和氧化/亚硝化应激的影响

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-01-01 DOI: 10.2478/rrlm-2021-0036

S. Avnioglu, M. Güngör, E. Kurutaş, Unal Ozturk, I. Demirhan, S. Bakarış, H. A. Velioglu, Seyda Cankaya, B. Yulug

{"title":"The Effect of Resveratrol on Sphingosine-1 and Oxidative/ Nitrosative Stress in an Experimental Heart Ischemia Reperfusion Model","authors":"S. Avnioglu, M. Güngör, E. Kurutaş, Unal Ozturk, I. Demirhan, S. Bakarış, H. A. Velioglu, Seyda Cankaya, B. Yulug","doi":"10.2478/rrlm-2021-0036","DOIUrl":"https://doi.org/10.2478/rrlm-2021-0036","url":null,"abstract":"Abstract Objectives: Resveratrol (RSV) is a natural polyphenolic compound showing significant antioxidant effects. In this study, we aimed to investigate the effects of resveratrol on the sphingosine-1-phosphate (S1P) and oxidative stress biomarkers in hearth ischemia-reperfusion (I/R). Materials and Methods: The biochemical and histopathological effects of RSV on cardiac ischemia-reperfusion injury were investigated through ELISA- and light microscope. Results: We observed statistically significant differences between the treatment group and the control group in terms of malondialdehyde (MDA) level, catalase (CAT) and superoxide dismutase (SOD) activities (p<0.05). Histopathologically, we also observed decreased Polymorphonuclear Leucocyte (PMNL) infiltration, myocardial edema, miyositolysis in the treatment group compared to the I/R and sham groups. Conclusion: Resveratrol may play an important role in cardiac I/R injury through its anti-inflammatory and antioxidant effects which were biochemically and histopathologically confirmed in the present study.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"28 1","pages":"9 - 18"},"PeriodicalIF":0.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89397423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Expression of tight junction transmembrane protein Claudin-1 in gastric carcinoma and effects on tumor cell proliferation, invasion and migration 紧密连接跨膜蛋白Claudin-1在胃癌中的表达及对肿瘤细胞增殖、侵袭和迁移的影响

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-01-01 DOI: 10.2478/rrlm-2022-0007

J. Lv, Z. She, A. Cai

{"title":"Expression of tight junction transmembrane protein Claudin-1 in gastric carcinoma and effects on tumor cell proliferation, invasion and migration","authors":"J. Lv, Z. She, A. Cai","doi":"10.2478/rrlm-2022-0007","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0007","url":null,"abstract":"Abstract Background: Claudin-1 is involved in various cancers, but its expression and role in gastric carcinoma remain unclear. Materials and Methods: Gastric carcinoma and adjacent normal tissues were harvested from 60 patients. Claudin-1 expression was detected by RT-qPCR. The expressions in human gastric carcinoma MKN45, SGC7901 and MKN28 cells and immortalized human gastric epithelium GES-1 cells were determined by RT-qPCR and Western blotting. Claudin-1 was overexpressed in SGC7901 cells by lentiviral transfection, and they were divided into Control (untransfected), normal control (NC) (transfected with lentiviral vector) and Claudin-1 (transfected with Claudin-1 overexpression lentivirus) groups. The proliferation, invasion and migration of gastric carcinoma cells were detected through cell counting kit-8, Transwell and wound healing assays, respectively. The effects of Claudin-1 on the expressions of epithelial-mesenchymal transition (EMT) marker proteins E-cadherin and N-cadherin were detected by Western blotting. Ten 4-week-old male BALB/c nude mice were subcutaneously injected with lentivirus-treated SGC7901 cells to establish the transplanted tumor model, and the effect of overexpression of Claudin-1 was explored. Results: The expression of Claudin-1 in gastric carcinoma tissues was significantly lower than that in adjacent tissues (P<0.05). Overexpression of Claudin-1 significantly inhibited the proliferation, invasion and migration of SGC7901 cells, increased the expression of E-cadherin, and decreased that of N-cadherin (P<0.05). Overexpression of Claudin-1 in the mouse model significantly inhibited the growth of subcutaneous transplanted tumors (P<0.05). Conclusion: Claudin-1 has low expression in gastric carcinoma tissues. Overexpression of Claudin-1 inhibits the proliferation, invasion, migration, and EMT of gastric carcinoma cells, and subcutaneous tumorigenesis in nude mice.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"30 1","pages":"81 - 91"},"PeriodicalIF":0.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83389488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circulating amino acids as fingerprints of visceral adipose tissue independent of insulin resistance: a targeted metabolomic research in women 循环氨基酸作为独立于胰岛素抵抗的内脏脂肪组织指纹:一项针对女性的代谢组学研究

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2021-10-01 DOI: 10.2478/rrlm-2021-0033

A. Mureşan, A. Rusu, R. Pop, C. Vonica, N. Hancu, C. Bocsan, C. Socaciu, C. Bala, G. Roman

{"title":"Circulating amino acids as fingerprints of visceral adipose tissue independent of insulin resistance: a targeted metabolomic research in women","authors":"A. Mureşan, A. Rusu, R. Pop, C. Vonica, N. Hancu, C. Bocsan, C. Socaciu, C. Bala, G. Roman","doi":"10.2478/rrlm-2021-0033","DOIUrl":"https://doi.org/10.2478/rrlm-2021-0033","url":null,"abstract":"Abstract Introduction: Although obesity and its biomarkers have been intensively studied, little is known about the metabolomic signature of visceral adiposity independent of insulin resistance that frequently accompanies increased levels of visceral fat. Our study aimed to investigate specific changes in amino acid (AA) levels as biomarkers of increased visceral adiposity independent of insulin resistance, in healthy subjects. Methods: Forty-two adult women were included in this cross-sectional study. Serum samples were analyzed by AAs targeted metabolomics according to their visceral fat area (<100 cm2 and ≥100 cm2). Results: By corrected t-test and supervised partial least-squares discriminant analysis (PLS-DA) we identified 4 AAs that were significantly higher in the group with higher visceral fat: proline (variable importance in the projection [VIP] predicted value: 1.97), tyrosine (VIP: 2.21), cysteine (VIP: 1.19), isoleucine (VIP: 1.04; p-values <0.05). Also, glycine was significantly lower in the group with higher visceral fat (VIP: 1.65; p-value <0.05). All AAs identified were associated with visceral fat independent of homeo-static model assessment for insulin resistance (p-value for regression coefficients <0.05). Conclusion: Metabolic pathways that might be disrupted in persons with increased visceral fat are phenylalanine, tyrosine, and tryptophan biosynthesis; tyrosine metabolism; glycine, serine, and threonine metabolism; glyoxylate and dicarboxylate metabolism, and cysteine and methionine metabolism.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"1987 1","pages":"439 - 451"},"PeriodicalIF":0.5,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87811804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0