Revista Romana De Medicina De Laborator最新文献_第5页

Correlations of cofilin1 and phosphorylation at Ser3 site with sensitivity of elderly patients with non-small cell lung cancer to radiotherapy cofilin1和Ser3位点磷酸化与老年非小细胞肺癌放疗敏感性的相关性

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-10-01 DOI: 10.2478/rrlm-2022-0034

Feijie Lu, Chunrong Zhong, Yongquan Dong, Mingming Wang, Qi Yang

{"title":"Correlations of cofilin1 and phosphorylation at Ser3 site with sensitivity of elderly patients with non-small cell lung cancer to radiotherapy","authors":"Feijie Lu, Chunrong Zhong, Yongquan Dong, Mingming Wang, Qi Yang","doi":"10.2478/rrlm-2022-0034","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0034","url":null,"abstract":"Abstract Background: To explore the correlations of cofilin1 (CFL1) and phosphorylation level of locus serine residue at position 3 (Ser3) with the sensitivity of elderly patients with non-small cell lung cancer (NSCLC) to radiotherapy. Methods: A total of 102 eligible patients treated from June 2013 to April 2015 were selected. The cases of complete remission and partial remission were included into radiotherapy-sensitive group (n=55), while those of stable disease and progressive disease were enrolled into radiotherapy-resistant group (n=47). Before treatment, tissues were collected to detect the expressions of CFL1 and CFL1 (phospho S3) by immunohistochemistry. The survival time and rate were recorded during follow-up. Results: Compared with the radiotherapy-sensitive group, the radiotherapy-resistant group had advanced tumor-node-metastasis (TNM) stage and higher lymph node metastasis rate (P=0.000, 0.000). Compared with the tissues with negative CFL1 expression, the tissues with positive CFL1 expression had advanced TNM stage and higher lymph node metastasis rate (P=0.013, 0.000). The positive expression rate of CFL1 in the radiotherapy-resistant group was higher than that of the radiotherapy-sensitive group, whereas the positive expression rate of CFL1 (phospho S3) in the former was lower (P=0.000, 0.000). Lymph node metastasis, high CFL1 expression, and low CFL1 (phospho S3) expression were independent predictors for resistance to radiotherapy (P=0.001, 0.006, 0.003). In the radiotherapy-sensitive group, the patients with negative CFL1 expression and positive CFL1 (phospho S3) expression had long progression-free survival and high 5-year survival rate (P=0.000, 0.000). Conclusion: The sensitivity to radiotherapy of elderly NSCLC patients is correlated negatively with CFL1 and positively with phosphorylation at locus Ser3. CFL1 and phosphorylation at locus Ser3 are independent predictors for sensitivity to radiotherapy.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"66 1","pages":"379 - 388"},"PeriodicalIF":0.5,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89032789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbapenem resistance determinants in Klebsiella pneumoniae strains isolated from blood cultures-comparative analysis of molecular and phenotypic methods 血培养分离的肺炎克雷伯菌碳青霉烯类耐药决定因素——分子和表型方法的比较分析

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-07-01 DOI: 10.2478/rrlm-2022-0027

M. Tompa, M. Iancu, S. Pandrea, M. Grigorescu, M. Ciontea, Ronald Tompa, Stanca M. Pandrea, L. Junie

{"title":"Carbapenem resistance determinants in Klebsiella pneumoniae strains isolated from blood cultures-comparative analysis of molecular and phenotypic methods","authors":"M. Tompa, M. Iancu, S. Pandrea, M. Grigorescu, M. Ciontea, Ronald Tompa, Stanca M. Pandrea, L. Junie","doi":"10.2478/rrlm-2022-0027","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0027","url":null,"abstract":"Abstract Introduction: This study provides data on carbapenemases identified in carbapenem-resistant Klebsiella pneumoniae (CR-KP) isolated from blood-cultures by the multiplex molecular method. Material and method: Between October 2016 and September 2017, 47 non-duplicate Klebsiella pneumoniae (KP) were isolated from blood cultures, from hospitalized patients in the Regional Institute of Gastroenterology and Hepathology, Cluj-Napoca, Romania. Identification and antimicrobial susceptibility tests (AST) were performed by Vitek 2 Compact. The combination disks test (CDT) was used for phenotypic analysis and the LightCycler® Multiplex DNA assay was used to detect and identify the carbapenemases by the LightCycler®z 480 Instrument. The following targets were chosen: blaKPC, blaNDM, blaGES, blaIMP and blaOXA-48 genes and the Cobas® 4800 software variant 2.2.0 was used for the results interpretation. Results: Taking into consideration the meropenem minimum inhibitory concentration (MIC), 29 KP were susceptible and 18 were not-susceptible (MIC≥0.5 µg ml-1). In the CR-KP group, the CDT identified OXA-48 (10/18) and KPC (7/18) producers. One isolate showed a noninterpretable profile. The multiplex molecular analyses confirmed the carbapenemases production as: 9 CR-KP were KPC and OXA-48 co-producers, 8 were OXA-48 and one was KPC producing strains. In CR-KP group, we found a significant correlation between the CDT and RT-PCR tests results, concerning KPC (p = 0.671). Eight phenotypic results were confirmed by molecular Light-Cycler® Multiplex DNA assay. For CR-KP co-producers (KPC and OXA-48), the CDT could indicate only one carbapenem-hydrolyzing enzyme. Conclusion: This study highlights the CR-KP co-producers (OXA-48 and KPC). OXA-48-like is more frequently encountered in our area than other carbapenemases.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"34 1","pages":"315 - 326"},"PeriodicalIF":0.5,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82227103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Associations of serum vitamin D and Fok I polymorphism of receptor gene with unexplained recurrent spontaneous abortion 血清维生素D和受体基因Fok I多态性与不明原因复发性自然流产的关系

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-07-01 DOI: 10.2478/rrlm-2022-0024

Hebin Liu, Yujuan Song, M. Zhou, Xiaoling Yao

{"title":"Associations of serum vitamin D and Fok I polymorphism of receptor gene with unexplained recurrent spontaneous abortion","authors":"Hebin Liu, Yujuan Song, M. Zhou, Xiaoling Yao","doi":"10.2478/rrlm-2022-0024","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0024","url":null,"abstract":"Abstract Background: To investigate the associations of serum vitamin D and Fok I polymorphism of its receptor (VDR) with unexplained recurrent spontaneous abortion (URSA). Methods: Ninety URSA patients and another 104 healthy pregnant women were selected as URSA and control groups, respectively. 25-Hydroxyvitamin D [25-(OH)D] level was detected by chemiluminescence. VDR gene Fok I polymorphism was analyzed by PCR, and the distribution of genotype frequency was calculated by Hardy-Weinberg equilibrium test. Association between Fok I polymorphism and susceptibility to URSA was investigated by logistic regression analysis. Results: Gestational age, uterine height, waist circumference, 25-(OH)D level and proportions of Fok I FF and Ff genotypes were significantly lower in the URSA group (P<0.05). Compared with ff genotype, risk of URSA reduced for Ff and FF genotypes. Compared with allele f, risk of URSA was lower for allele F. 25-(OH)D level of ff genotype was significantly lower in the URSA group, which was lower than that of FF genotype (P<0.05). Compared with women with 25-(OH)D level >30 ng/mL and F allele (FF+Ff), the risk of URSA increased 2.45-, 2.43- and 5.34-fold for those with 25-(OH)D level >30 ng/mL and ff genotype, with 25-(OH)D level ≤30 ng/mL, and with ff genotype and 25-(OH)D level ≤30 ng/mL, respectively. Conclusions: The 25-(OH)D level of the URSA group was significantly lower than that of normal pregnant women. Probably, VDR gene Fok I polymorphism is associated with URSA occurrence, and allele F decreases the risk. The risk of URSA dramatically increases in women with ff genotype and 25-(OH)D deficiency.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"2006 1","pages":"293 - 304"},"PeriodicalIF":0.5,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90504707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Normality assessment, few paradigms and use cases 常态性评估，少量范例和用例

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-07-01 DOI: 10.2478/rrlm-2022-0030

C. Avram, M. Mărușteri

{"title":"Normality assessment, few paradigms and use cases","authors":"C. Avram, M. Mărușteri","doi":"10.2478/rrlm-2022-0030","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0030","url":null,"abstract":"Abstract Background: The importance of applying the normality tests is underlined by the way of continuing the statistical protocol for numerical data within inferential statistics, respectively by the parametric or non-parametric tests that we will apply further on. Methods: To check the calculation mode, we used sets of random values and we performed the normality assessment using statistical calculation programs. We took non-Gaussian data (n = 30, n = 50, n = 100, n = 500) and Gaussian data (n = 30, n = 50, n = 100, n = 500) for which we checked the normality of the data. Data chosen for this study were most representative for each batch (n). Results: The application of normality tests to the data under study confirms that the data are non-Gaussian for the first data set. For the Gaussian data sample, the verification of normality is confirmed by the results. Conclusion: For data up to 50 subjects, it is recommended to apply the Shapiro-Wilk test, but also to apply graphical methods to confirm the accuracy of the result. If the data samples have more than 50 values, the D’Agostino & Pearson omnibus normality test should be applied and if the statistical program does not contain this test, the Shapiro-Wilk test can be applied (in the case of SPSS). Graphical methods, although they require some experience, are useful for identifying the normality of distributions with a small number of data.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"51 1","pages":"251 - 260"},"PeriodicalIF":0.5,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87886271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Elbow Septic Arthritis caused by Klebsiella pneumoniae in a Newborn - Case Report and Literature Review 新生儿肺炎克雷伯菌所致肘关节脓毒性关节炎1例报告及文献复习

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-07-01 DOI: 10.2478/rrlm-2022-0029

Roxana Filip, Dana Georgeta Laura Murariu, Ramona Avramia, F. Filip

引用次数: 0

Novel DCX pathogenic variant in a girl with subcortical band heterotopia 一名皮质下带异位女孩的DCX新致病变异

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-07-01 DOI: 10.2478/rrlm-2022-0031

S. Papuc, M. Budisteanu, A. Erbescu, V. Ionescu, C. Iliescu, C. Sandu, A. Arghir

{"title":"Novel DCX pathogenic variant in a girl with subcortical band heterotopia","authors":"S. Papuc, M. Budisteanu, A. Erbescu, V. Ionescu, C. Iliescu, C. Sandu, A. Arghir","doi":"10.2478/rrlm-2022-0031","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0031","url":null,"abstract":"Abstract Subcortical band heterotopia (SBH), is a brain malformation defined by symmetrical and bilateral heterotopic gray matter bands localized deep within the white matter, between the cortex and lateral ventricles. SBH is the result of abnormal neuronal migration, with improper positioning of the cortical neurons. DCX gene (doublecortin), a microtubule-associated protein with essential roles in neuronal migration and differentiation during brain development, is one of the main contributors to the X-linked Lissencephaly spectrum pathogenesis (OMIM #300067). DCX variants are responsible for SBH in females and isolated lissencephaly in males. Herein, we present a 7-year-old girl with a de novo frameshift variant in DCX gene, unreported by date. The patient has focal complex seizures with onset at 23 months of age, fully controlled with medication, mild tremor and coordination impairment of fine movements and some learning difficulties, otherwise with normal development. The brain magnetic resonance imaging revealed the presence of thick SBH. Direct sequencing of DCX gene revealed a pathogenic heterozygous cytosine duplication in exon 3; this frameshift variant leads to a premature stop codon in position 164 (p.Gln160Profs*5). The variant type and its predicted consequence at protein level correlates with the severity of radiological findings. The clinical presentation of our patient is, however, milder than expected. Our research expands the mutational spectrum of DCX gene in SBH females and provides a detailed clinical and imagistic description of the patient. This paper highlights the utility of single gene sequencing as a first-tier diagnostic test of patients with gene-specific phenotypic features.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"62 1","pages":"345 - 352"},"PeriodicalIF":0.5,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76639849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Downregulation of hsa-miR-4328 and target gene prediction in Acute Promyelocytic Leukemia hsa-miR-4328在急性早幼粒细胞白血病中的下调及靶基因预测

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-07-01 DOI: 10.2478/rrlm-2022-0022

O. T. Lupu, B. Popescu, Elena Avram, M. Dragomir, Gheorghe Dănuț Cimponeriu, Ioana Mighiu, Silvia Aposteanu, D. Coriu

{"title":"Downregulation of hsa-miR-4328 and target gene prediction in Acute Promyelocytic Leukemia","authors":"O. T. Lupu, B. Popescu, Elena Avram, M. Dragomir, Gheorghe Dănuț Cimponeriu, Ioana Mighiu, Silvia Aposteanu, D. Coriu","doi":"10.2478/rrlm-2022-0022","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0022","url":null,"abstract":"Abstract Introduction: Acute promyelocytic leukemia (APL) is defined by the PML-RARA fusion gene. APL treatment can have significant side effects, therefore the development of optimal therapeutic options is crucial. Although the study of miRNAs is still in its infancy, it has been shown that these molecules are involved in the pathogenesis of neoplasms by modulating the expression of target genes. miRNAs can be considered possible biomarkers in APL and can be used as therapeutic targets or as markers for the therapeutic response. Objectives: The purpose of this study was to determine whether differentially expressed putative miRNAs that have RARA as a target gene could be considered reliable biomarkers for APL. Methods: Using bioinformatics tools, a panel of 6 miRNAs with possible tropism for the RARA gene was selected from miRDB. We evaluated their expression levels in samples from patients with APL (n=20) or from healthy subjects without mutations in genes associated with leukemia or myeloproliferative diseases (n=21). Results: All 6 putative miRNAs were identified using electrophoresis (hsamir-4299, hsa-mir-4328, hsa-mir-7851-3p, hsa-mir-6827-5p, hsa-mir-6867-5p, hsa-mir-939-5p). Of the six miRNAs, hsa-mir-4328 is deeply downregulated in subjects diagnosed with APL compared to healthy subjects, whereas hsa-mir-4299 and hsa-mir-7851-3p show small differences in expression between the two study groups, but without statistical significance. Our results suggest that hsa-mir-4328 may have a role in the pathogenesis of APL and may represent a new biomarker for this type of leukemia. Key Words: miRNA, APL, leukemia, bioinformatics.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"63 1","pages":"261 - 272"},"PeriodicalIF":0.5,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83158657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Expression of serum soluble Klotho protein in patients with renal damage induced by anti-neutrophil cytoplasmic antibody-associated small-vessel vasculitis and influence on prognosis 抗中性粒细胞细胞质抗体相关性小血管炎肾损害患者血清可溶性Klotho蛋白表达及对预后的影响

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-07-01 DOI: 10.2478/rrlm-2022-0021

Feiju Ma, Jianfei Li

{"title":"Expression of serum soluble Klotho protein in patients with renal damage induced by anti-neutrophil cytoplasmic antibody-associated small-vessel vasculitis and influence on prognosis","authors":"Feiju Ma, Jianfei Li","doi":"10.2478/rrlm-2022-0021","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0021","url":null,"abstract":"Abstract Background: Anti-neutrophil cytoplasmic antibody-associated small-vessel vasculitis (AASV) is an autoimmune disease with unclear pathogenesis, which causes damage to multiple organs and systems, renal failure or even death. We aimed to explore the expression of serum soluble Klotho protein in patients with AASV-induced renal damage and influence on prognosis. Methods: A total of 330 AASV patients treated from June 2012 to June 2014 were divided into renal damage and non-renal damage groups. Clinical symptoms and laboratory examination results were compared. They were divided into Klotho <935.05 pg/mL and ≥935.05 pg/mL groups, and renal damage and pathological indices were compared. Survival curves were plotted using Kaplan-Meier method, and 5-year and renal survival rates were compared. Results: Compared with the non-renal damage group, the mean arterial pressure, urine protein and blood creatinine levels significantly rose, while the red blood cell count, hemoglobin, serum albumin, and Klotho protein levels declined in the renal damage group (P<0.05). The optimal cut-off value of Klotho protein in assessing renal damage was 935.05 pg/mL. Compared with Klotho ≥935.05 pg/mL group, the levels of blood creatinine and urine protein significantly increased, and the proportion of normal glomeruli decreased, while that of fibrous crescents rose in Klotho <935.05 pg/mL group (P<0.05). The 5-year renal survival rate was significantly lower in Klotho <935.05 pg/mL group than that in Klotho ≥935.05 pg/mL group (P<0.05). Conclusions: Klotho protein is lowly expressed in patients with renal damage induced by AASV as a potential marker for early diagnosis and prognostic evaluation.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"16 1","pages":"305 - 314"},"PeriodicalIF":0.5,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87854885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expressions of TGF-β1 and VEGF in patients with acute myeloid leukemia and associations with prognosis TGF-β1和VEGF在急性髓性白血病患者中的表达及其与预后的关系

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-07-01 DOI: 10.2478/rrlm-2022-0026

Yan Xu, Xian-qiu Yu, Xin-long Zhang

{"title":"Expressions of TGF-β1 and VEGF in patients with acute myeloid leukemia and associations with prognosis","authors":"Yan Xu, Xian-qiu Yu, Xin-long Zhang","doi":"10.2478/rrlm-2022-0026","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0026","url":null,"abstract":"Abstract Background: To study the expressions of transforming growth factor-β1 (TGF-β1) and vascular endothelial growth factor (VEGF) in patients with acute myeloid leukemia (AML) and their values for prognosis. Methods: A total of 120 AML patients treated from January 2015 to December 2018 were selected. Bone marrow mononuclear cells were isolated. The expressions of TGF-β1 and VEGF were detected by RT-PCR, and their associations with clinical characteristics were analyzed. The overall survival (OS) and disease-free survival (DFS) were assessed using the Kaplan-Meier method. The risk factors for prognosis were analyzed through the Cox proportional hazards model. Results: The AML group had significantly lower relative expression of TGF-β1 and higher relative expression of VEGF than those of the control group (P<0.05). TGF-β1 and VEGF levels were significantly correlated with white blood cell count, hemoglobin, platelets, and peripheral blood juvenile cells (P<0.05). TGF-β1 level was higher and VEGF level was lower in the patients with complete response than those in the patients with partial response and no response (P<0.05). Both OS and DFS of the patients with high TGF-β1 expression were better than those of the patients with low TGF-β1 expression, while they were also superior among the patients with low VEGF expression (P<0.05). Platelets, TGF-β1 and VEGF were independent influencing factors for OS, and white blood cells, TGF-β1 and VEGF were independent influencing factors for DFS (P<0.05). Conclusions: AML patients have decreased expression of TGF-β1 and increased expression of VEGF, and such changes are closely associated with the prognosis of AML.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"108 1","pages":"273 - 280"},"PeriodicalIF":0.5,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74646609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SPP1 is a biomarker of cervical cancer prognosis and involved in immune infiltration SPP1是宫颈癌预后的生物标志物，参与免疫浸润

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-07-01 DOI: 10.2478/rrlm-2022-0028

Qian Guo, W. He, D. Nie, Wuzhi Li, P. Zhan

{"title":"SPP1 is a biomarker of cervical cancer prognosis and involved in immune infiltration","authors":"Qian Guo, W. He, D. Nie, Wuzhi Li, P. Zhan","doi":"10.2478/rrlm-2022-0028","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0028","url":null,"abstract":"Abstract Background: Cervical cancer is the fourth commonly occurred cancer in women around the world. However, it still lacks effective approaches to improve current prognosis of cervical cancer and prevent metastasis. Objective: We aim to discover a promising biomarker for cervical cancer prognosis by utilizing bioinformatics analysis. Methods: Gene expression was analyzed by the datasets from The Cancer Genome Atlas Program-Cervical squamous cell carcinoma and endocervical adenocarcinoma (TCGA-CESC) dataset and three independent patient cohort datasets. Biological process and pathway enrichment were performed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analysis. Immune infiltration was analyzed through TISIDB tool. Results: SPP1 gene was highly expressed in cervical cancer tissues. In addition, SPP1 was positively correlated to advanced CESC stages and nodal metastasis status. SPP1 co-expressed genes are mainly enriched in immunological processes. Furthermore, SPP1 expression is involved in immune infiltration level, in which several tumour infiltrating lymphocytes are correlated with SPP1. SPP1 overexpression promotes a wide spectrum of chemokines and immunoinhibiors which contribute to CESC progression. Conclusions: SPP1 is a promising biomarker and a prognostic factor of CESC. Tumour infiltrating lymphocytes are also possibly regulated by SPP1. Our study suggests that investigation on SPP1 is a new direction for CESC therapy.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"220 1","pages":"281 - 292"},"PeriodicalIF":0.5,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77781974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0