Revista Romana De Medicina De Laborator最新文献_第4页

The metabolic control and laboratory evaluation in patients with type 2 diabetes during the COVID-19 pandemic and the impact of telemedicine: a single-center experience 新冠肺炎大流行期间2型糖尿病患者的代谢控制和实验室评估及远程医疗的影响:单中心体验

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2023-01-01 DOI: 10.2478/rrlm-2023-0002

Corina Roxana Onea, Ákos Erőss, Andrada Larisa Roiban, S. Cernea

{"title":"The metabolic control and laboratory evaluation in patients with type 2 diabetes during the COVID-19 pandemic and the impact of telemedicine: a single-center experience","authors":"Corina Roxana Onea, Ákos Erőss, Andrada Larisa Roiban, S. Cernea","doi":"10.2478/rrlm-2023-0002","DOIUrl":"https://doi.org/10.2478/rrlm-2023-0002","url":null,"abstract":"Abstract Objective: The study aimed to evaluate the metabolic control during the COVID-19 pandemic in subjects with type 2 diabetes mellitus (T2DM) and whether care through telemedicine significantly impacted it. Material and methods: This was a retrospective study for which three time-periods were prespecified: the COVID-19 pandemic period, pre-COVID-19 period, and before pre-COVID-19. The following information were collected: anthropometric and laboratory parameters (glycated hemoglobin (HbA1c), blood glucose, lipid profile, creatinine, eGFR, etc.), self-measured blood glucose (SMBG), blood pressure (BP), diabetes therapy, number of on-site and of telemedicine consultations. The mean values of all available measurements for HbA1c, SMBG, BP, weight, and body mass index (BMI) were calculated. Results: During the COVID-19 pandemic, the HbA1c values increased (6.78±0.77% to 6.96±0.87%, p<0.0001; + 0.18±0.67%), despite treatment intensification (p<0.01), while BMI and total cholesterol values slightly decreased (32.01±5.5 kg/m2 to 31.7±5.4 kg/m2, p<0.0001, and 178.1±40.8 mg/dl to 170.5±38.4 mg/dl, p<0.05). The deterioration of glycemic control (to HbA1c >7%) were rather seen in males (to 7.01±0.92%, p<0.0001), patients from rural areas (to 7.08±0.96%, p<0.001) and younger than 65 y.o. (to 7.05±0.82%, p<0.001). More male patients had a mean HbA1c increase of >0.5% during the COVID-19 pandemic (32.3% vs 21.5%, p<0.05). Patients who received more diabetes care visits through telemedicine had a more attenuated increase in HbA1c (6.79±0.80% to 6.90±0.83%, p<0.05 vs 6.76±0.72% to 7.08±0.92%, p<0.0001). Conclusions: The glycemic control slightly deteriorated during the COVID-19 pandemic (but with overall HbA1c within target), with certain patient categories being more affected. Diabetes care though telemedicine allowed the maintenance of the metabolic control.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"47 1","pages":"43 - 50"},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75075657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Setting up an own laboratory performance-based internal quality control plan - a model for complete blood count 建立基于实验室绩效的内部质量控制计划——全血细胞计数模型

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-10-01 DOI: 10.2478/rrlm-2022-0036

O. Oprea, Elena Cristina Preda, I. Mănescu, M. Dobreanu

{"title":"Setting up an own laboratory performance-based internal quality control plan - a model for complete blood count","authors":"O. Oprea, Elena Cristina Preda, I. Mănescu, M. Dobreanu","doi":"10.2478/rrlm-2022-0036","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0036","url":null,"abstract":"Abstract Quality Control (QC) in Romania is regulated by the Order of the Minister of Health no. 1608/2022 that modifies the previous Order 1301/2007. The new version of the Order introduces a more scientific approach by requesting the laboratories to assess test performance and then elaborate an appropriate internal QC plan. The aim of this study was to demonstrate how to design a QC plan for complete blood count (CBC) in an Emergency Laboratory with continuous activity, in order to comply with the new Order 1608/2022. QC data obtained over a three-month period (April-June 2022) from the Sysmex XN-1000 instrument of the Emergency Laboratory of the County Emergency Clinical Hospital of Târgu Mureș were included. In order to establish an appropriate QC plan, two models were applied and the following parameters were calculated: the number of daily QC runs (N), the probability of false rejection (Pfr), the QC frequency (run size), and the required QC rules. White blood cells achieved high performance, while Hematocrit performance was poor. Different levels of performance were achieved for Platelets. We emphasize that, when all parameters are measured on the same instrument, QC frequency and Pfr should be adjusted in order to develop a QC plan that “fits” all the parameters of the CBC as a whole. In our Emergency Laboratory, the calculated QC plan for CBC is N=2, Pfr=0.03, multi-rule 1:3s/2:2s/R:4s, and a run size of 95 samples which is approximately the same as the number of CBCs performed during one 12-hour shift.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"1 1","pages":"477 - 482"},"PeriodicalIF":0.5,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79831395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Protocol and Matters for Consideration for the Treatment of Polymerase Chain Reaction Contamination in Next-Generation Sequencing Laboratories 新一代测序实验室聚合酶链反应污染处理的方案和考虑事项

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-10-01 DOI: 10.2478/rrlm-2022-0038

Lin Wang, Chen Ma, Bin He, R. Qiang, Hongsu Zhang

{"title":"Protocol and Matters for Consideration for the Treatment of Polymerase Chain Reaction Contamination in Next-Generation Sequencing Laboratories","authors":"Lin Wang, Chen Ma, Bin He, R. Qiang, Hongsu Zhang","doi":"10.2478/rrlm-2022-0038","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0038","url":null,"abstract":"Abstract Objective: The contamination of polymerase chain reaction (PCR) samples in molecular diagnostic laboratories can cause serious consequences. Internal quality control efforts are often inadequate, especially in clinical next-generation sequencing (NGS) laboratories. Methods: In this study, we retrospectively investigated an incidence of PCR contamination and its decontamination process in a clinical laboratory. We performed a series of measures for decontamination. Taqman fluorescence quantification was carried out to determine the presence of contaminating DNA. SYBR-Green PCR was conducted to evaluate the effect of chlorine disinfectant on NGS library preparation. Results: Through a series of elimination measures undertaken over 8 weeks, the decontamination process was verified as reliable. Almost no contamination was detected. Chlorine disinfectant should be forbidden in Illumina NGS laboratories because it may cause the failure of library preparation. Conclusion: Our prevention and decontamination strategies could effectively eliminate PCR amplicons. Chlorine disinfectants should not be used in Illumina NGS laboratories.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"77 1","pages":"467 - 475"},"PeriodicalIF":0.5,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90685406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Endothelial progenitor cell (EPCs)-derived exosomal miR-30d-5p inhibits the inflammatory response of high glucose-impaired fibroblasts by affecting the M1/M2 polarization of macrophages 内皮祖细胞(EPCs)来源的外泌体miR-30d-5p通过影响巨噬细胞的M1/M2极化抑制高糖受损成纤维细胞的炎症反应

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-10-01 DOI: 10.2478/rrlm-2022-0032

Wu Xiong, Meiling Tan, Ziran Chen, Yu Liu, Yang Liu, X. Zou, Xiao-qin Wang, Ya Yang, Pei Tan, Xi Zhang

{"title":"Endothelial progenitor cell (EPCs)-derived exosomal miR-30d-5p inhibits the inflammatory response of high glucose-impaired fibroblasts by affecting the M1/M2 polarization of macrophages","authors":"Wu Xiong, Meiling Tan, Ziran Chen, Yu Liu, Yang Liu, X. Zou, Xiao-qin Wang, Ya Yang, Pei Tan, Xi Zhang","doi":"10.2478/rrlm-2022-0032","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0032","url":null,"abstract":"Abstract Background: Diabetes is a common chronic disease which has caused a great burden on families and society. The treatment of diabetes has always been a hotspot. This study aimed to explore the effect and mechanism of miR-30d-5pon inflammation of high glucose-impaired human keloid fibroblasts (HKF). Methods: Differently-expressed miRNAs were predicted by bioinformatics methods. Exosomes were observed by transmission electron microscope. Exosome particle sizes were measured by NanoSight. Western Blot was used to detect the expression of CD81, CD63, CD9, and Calnexin. QRT-PCR was used to detect the expression of miR-30d-5p, IL-1β, TNF-α, VEGF, FGF21, NRF2, and HO-1. The levels of IL-1β, TNF-α, IL-6, IL-10, and TGF-β were determined by ELISA. Cell apoptosis and CD86, CD206 positive cells were detected by flow cytometry. Results: Tori formula could promote the secretion of endothelial progenitor cell (EPCs) exosomes. EPCs exosomes and miR-30d-5p could stimulate the proliferation of HKF impaired by high glucose and the expression of IL-10 and TGF-β. MiR-30d-5p inhibited the proliferation of M1 macrophages and the expression of IL-1β and TNF-α. It could also promote the proliferation of M2 macrophages and the expression of CCL17 and CCL22. Moreover, miR-30d-5p stimulated the expression of VEGF, FGF21, NRF2, and HO-1, as well as suppressed the expression of IL-1β, TNF-α, and IL-6. MiR-30d-5p also restrained the apoptosis of impaired HKF. Conclusion: This study confirmed that miR-30d-5p could promote the M1/M2 polarization and inhibit the inflammatory response of impaired HKF, which provided a certain idea and direction for treating diabetes.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"30 1","pages":"435 - 451"},"PeriodicalIF":0.5,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79720849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The effect of Ulva rigida (C. Agardh, 1823) against cadmium-induced apoptosis and oxidative stress 芦笋(C. Agardh, 1823)对镉诱导的细胞凋亡和氧化应激的影响

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-10-01 DOI: 10.2478/rrlm-2022-0016

L. C. Irkin

{"title":"The effect of Ulva rigida (C. Agardh, 1823) against cadmium-induced apoptosis and oxidative stress","authors":"L. C. Irkin","doi":"10.2478/rrlm-2022-0016","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0016","url":null,"abstract":"Abstract Cadmium (Cd) is known as a pollutant source in recent years with the increase in industrialization. Algae have secondary metabolites with high biological activity, used for pharmaceutical agents. The liver and kidney are the primary organs involved in the elimination of systemic cadmium and the main targets of cadmium toxicity. In the previous research, it was determined the ameliorative effects of the extract obtained from Ulva rigida in the liver tissue of rats induced by cadmium. 35 female Wistar rats between 225-240 g were used. The subjects were injected subcutaneously with 1 mg/kg cadmium chloride (CdCl2) four times a week for four weeks. The study was carried out by groups as control (G1), Cadmium group (1 mg/kg CdCl2-G2), Algae group (100 mg/kg-G3), Cd+algae group (1 mg/kg CdCl2+50 mg/kg algae extract-G4) and Cd+algae group (1 mg/kg CdCl2+100 mg/kg algae extract -G5). The subjects were sacrificed by cervical dislocation. Liver tissue and cardiac blood were collected. It was determined that oxidative stress with iNOS, inflammation and apoptosis with TNF-α increased with cadmium induction, while there was a statistically significant decrease in the groups that were given algae extract. In addition, biochemical changes in SOD, CAT and MDA values were found to be significant (p<0.05). As a result, it was determined that algae extract could play a protective role with its antioxidant and antiapoptotic properties in experimentally induced cadmium toxicity in rats.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"27 1","pages":"423 - 433"},"PeriodicalIF":0.5,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76737099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic value of combination of exfoliative cytology with CA125, CEA, NSE, CYFRA21-1 and CA15-3 for lung cancer 剥脱细胞学联合CA125、CEA、NSE、CYFRA21-1、CA15-3对肺癌的诊断价值

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-10-01 DOI: 10.2478/rrlm-2022-0037

Liang-liang Zhang, Jun Lei, Jing Xia, A. Cai

{"title":"Diagnostic value of combination of exfoliative cytology with CA125, CEA, NSE, CYFRA21-1 and CA15-3 for lung cancer","authors":"Liang-liang Zhang, Jun Lei, Jing Xia, A. Cai","doi":"10.2478/rrlm-2022-0037","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0037","url":null,"abstract":"Abstract Background: To explore the diagnostic value of combination of exfoliative cytology with detection of tumor markers carbohydrate antigen 125 (CA125), carcinoembryonic antigen (CEA), neuron specific enolase (NSE), cytokeratin 19 fragment antigen 21-1 (CYFRA21-1) and CA15-3 for lung cancer. Methods: A total of 256 patients were enrolled, including 164 males and 92 females aged (64.51±22.68) years old. Among them, 189 patients (100 males and 89 females) were randomly selected as Tumor group, and the remaining 67 patients were used for validation. Another 514 healthy people receiving physical examination in our hospital during the same period were selected, from which 397 cases (266 males and 131 females) were randomly selected as No Tumor group, and the remaining 117 cases were used for validation. The biochemical criteria were detected in all subjects. The diagnostic value of each index for lung cancer was analyzed using receiver operating characteristic (ROC) curves. Results: The results of ROC curve analysis revealed that in Tumor group, the area under curve (AUC) of exfoliative cytology, CA125, CYFRA21-1, CA15-3, CEA and NSE was ≥0.7, while that of CA72-4, CA19-9, TSGF, AFP, CA242, SCCAg and CA50 was <0.7. The indices in each factor were comprehensively assessed, and then exfoliative cytology, CA125, CA15-3, CYFRA21-1, CEA and NSE were screened to establish the lung cancer prediction model. The diagnostic value was comparable between the prediction model and the combined detection of 9 indices (Z=1.682, P=0.079). Conclusions: The lung cancer prediction model balances sensitivity and specificity without reducing the diagnostic efficiency.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"80 1","pages":"389 - 398"},"PeriodicalIF":0.5,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81500084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic Carbapenemase Production and blaOXA detecting by PCR in Acinetobacter baumannii isolates from a Hospital of Infectious Diseases from North-East Romania 罗马尼亚东北部某传染病医院鲍曼不动杆菌分离株碳青霉烯酶表型及blaOXA的PCR检测

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-10-01 DOI: 10.2478/rrlm-2022-0033

E. Buzilă, O. Dorneanu, C. Luncă, I. Jelihovschi, L. Iancu

{"title":"Phenotypic Carbapenemase Production and blaOXA detecting by PCR in Acinetobacter baumannii isolates from a Hospital of Infectious Diseases from North-East Romania","authors":"E. Buzilă, O. Dorneanu, C. Luncă, I. Jelihovschi, L. Iancu","doi":"10.2478/rrlm-2022-0033","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0033","url":null,"abstract":"Abstract Introduction: In the last 40 years, Acinetobacter baumannii has been among the bacteria known to acquire multiple mechanisms of antibiotic resistance and, as a result, it is now one of the pathogens involved in healthcare-associated infections with multidrug resistant strains. Our study aimed to assess the production of carbapenemases in carbapenem-resistant A. baumannii by means of phenotypic methods and polymerase chain reaction technique (PCR), as well as to appraise the performances of carbapenemase detection by phenotypic tests compared to the PCR approach. Materials and Methods: We used phenotypic methods (E-test MBL, CIM, MHT, Rosco® Kit/OXA/MBL, OXA-23 K-SeT® assay) to investigate the production of carbapenemases in 43 carbapenem-resistant A. baumannii isolates, and PCR to screen for the genes blaOXA-23, blaOXA-24, blaOXA-58, blaOXA-51, blaVIM, blaIMP and blaNDM. Results: The carbapenem inactivation method (CIM) at 2 hours, CIM at 4h, OXA-23 K-SeT® assay, Rosco® Kit/OXA, and modified Hodge test (MHT) identified 26%, 63%, 65%, 81%, and 42% carbapenemase-producing isolates, respectively. The phenotypic E-test MBL detected metallo-β-lactamase (MBL) production in 79% of strains. PCR revealed blaOXA-51 in all the isolates, blaOXA-23 in 35/43 (81%), blaOXA-24 in 28/43 (65%), blaVIM in 7/43 (3%) and blaOXA-58, blaIMP, blaNDM were not detected. Conclusion: Because phenotypic tests do not highlight all the carbapenemase-producing strains, their results must be interpreted with caution relative to their level of performance, and negative results should be confirmed by means of PCR.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"14 1","pages":"453 - 465"},"PeriodicalIF":0.5,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82023517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autoantibodies Against ROS-Human Serum Albumin-A Potent Immunological marker in Depressed Individuals with Smoking History 抗ros -人血清白蛋白的自身抗体-吸烟史抑郁症患者的有效免疫标志物

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-10-01 DOI: 10.2478/rrlm-2022-0039

S. Sherwani, M. Khan

{"title":"Autoantibodies Against ROS-Human Serum Albumin-A Potent Immunological marker in Depressed Individuals with Smoking History","authors":"S. Sherwani, M. Khan","doi":"10.2478/rrlm-2022-0039","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0039","url":null,"abstract":"Abstract Background: Depression is one of the significant problems in adults that accounts for up to five percent of cases worldwide. Methods: Volunteers were divided into eight groups, and their serum samples were tested for FBG, carbonyl contents, IFN-γ and TNF-α. Reactive oxygen species (ROS) modified human serum albumin (HSA) (ROSHSA) was used as an antigen and levels of serum autoantibodies were estimated by direct binding and inhibition ELISA in all subjects. Results: Significant biophysical structural modifications were observed in ROS-HSA with increased carbonyl contents compared to native-HSA (N-HSA). Significantly high levels of carbonyl content (2.68 ± 0.33 nmol/mg protein; p > 0.001) and pro-inflammatory cytokines IFN-γ (7.4 ± 0.61 pg/ml; p > 0.001) and TNF-α (1.47 ± 0.23 pg/ml; p > 0.001) were detected in serum samples from F-D-S. Similarly, a high level of autoantibodies against ROS-HSA was observed in females who were depressed and smokers (F-D-S) group (0.89 ± 0.07; p > 0.001) compared to males who were both depressed and smokers (M-D-S) (0.66 ± 0.049). Furthermore, inhibition ELISA results exhibited high recognition of serum autoantibodies from F-D-S subjects (78.6 ± 5.7 mean maximum percentage inhibition MMPI) compared to M-D-S (58.8 ± 5.2 MMPI) subjects. Conclusion: Incoherence, long term unchecked chronic psychological stress may cause oxidation of blood proteins, which subsequently result in structural alterations of biomolecules, thus generating new-epitopes, capable of inducing autoantibodies specific for ROS-modified proteins. These autoantibodies may be a potential marker for subjects suffering from depression to understand the state of immune imbalance.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"1 1","pages":"399 - 411"},"PeriodicalIF":0.5,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78361553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Oxidative stress and metabolic parameters in hypertensive patients with/without diabetes mellitus in cardiovascular risk evaluation 氧化应激和代谢参数在合并/不合并糖尿病的高血压患者心血管风险评价中的作用

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-10-01 DOI: 10.2478/rrlm-2022-0025

E. Nemes-Nagy, E. Fogarasi, M. Croitoru, Z. Preg, S. Pál, K. Nyulas, T. Pál, R. Tripon, M. Tilinca, M. Germán-Salló

{"title":"Oxidative stress and metabolic parameters in hypertensive patients with/without diabetes mellitus in cardiovascular risk evaluation","authors":"E. Nemes-Nagy, E. Fogarasi, M. Croitoru, Z. Preg, S. Pál, K. Nyulas, T. Pál, R. Tripon, M. Tilinca, M. Germán-Salló","doi":"10.2478/rrlm-2022-0025","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0025","url":null,"abstract":"Abstract Introduction: Hypertension and diabetes mellitus affect a large number of patients and can significantly influence their life expectancy. Changes in metabolic and oxidative stress parameters are common in these pathologies, contributing to associated complications. The aim of the study was assessment of relationship between laboratory parameters and their role in evaluation of cardiovascular risk, and possible gender-related differences in the protective factors. Material and methods: Blood samples were collected from hypertensive patients with/without diabetes mellitus admitted to the Cardiovascular Rehabilitation Clinic in Tîrgu Mureș and controls without these pathologies. Biochemical analyses were performed on Konelab analyzer (glycemia, lipid profile, kidney function tests, zinc, hsCRP). Oxidative stress markers, such as serum malondialdehyde (MDA), oxidized (GSSG) and reduced glutathione (GSH) were evaluated using an HPLC-UV/VIS technique at GEP UMPhST. Statistical analysis was performed by GraphPad InStat3. Results: Mean age of hypertensive patients (n=131) was 69.44 ± 9.02 years, 45.8% males, 31.3% being diabetics. 74.1% of the studied patients had zinc deficiency, 19.8% presented slightly elevated hsCRP. The control group included 24 nonhypertensive/nondiabetic patients of similar age. Average GSH was significantly lower (p=0.0002) in hypertensive patients, 1.89 ± 0.82 µg/ml, compared to the control group (3.23 ± 0.49 µg/ml), and no correlation could be observed between GSH and MDA values. GSH concentration was significantly higher in males (p=0.0395) and HDL-cholesterol significantly higher in females (p=0.0132). A negative correlation was observed between serum triglyceride and HDL-cholesterol concentration. Conclusions: Gender differences are present in the level of protective factors against cardiovascular diseases, while oxidative stress is intensified in hypertensive/diabetic patients.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"30 1","pages":"413 - 422"},"PeriodicalIF":0.5,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86003413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

High DNAJA4 expression correlates with poor survival outcomes in breast cancer 乳腺癌患者DNAJA4高表达与生存预后差相关

IF 0.5 4区医学

Revista Romana De Medicina De Laborator Pub Date : 2022-10-01 DOI: 10.2478/rrlm-2022-0035

T. Acun, Oya Incekara

{"title":"High DNAJA4 expression correlates with poor survival outcomes in breast cancer","authors":"T. Acun, Oya Incekara","doi":"10.2478/rrlm-2022-0035","DOIUrl":"https://doi.org/10.2478/rrlm-2022-0035","url":null,"abstract":"Abstract Background: DNAJA4 (PRO1472) is a heat shock protein that has been associated with several types of cancers, including breast cancer. We aimed to reveal the protein expression, clinical outcomes, and regulatory mechanisms of DNAJA4 gene in breast cancer by employing tissue microarrays, transcriptomic datasets, and in-silico tools. Methods: DNAJA4 protein expression and its clinical implications were evaluated by immunohistochemistry assay (normals = 32; tumors = 121). RNA-seq and DNA microarray datasets were analyzed by using breast cancer gene-expression miner (Bc-GenExMiner v4.8) to estimate the survival probabilities of breast cancer patients. DNAJA4 promoter methylation level was analyzed in clinical samples by UALCAN in-silico tool (normals = 97; tumors = 793). Results: DNAJA4 protein expression is significantly high in clinical breast cancer samples compared to the normal samples (P = 0.016). High DNAJA4 mRNA expression is correlated with poor overall survival (OS), disease-free survival (DFS), and distant metastasis-free survival (DMFS) in breast cancer patients (P < 0.05). Mutations or copy number variations of DNAJA4 are uncommon in clinical samples. Reduced promoter methylation was observed in clinical breast cancer samples. Conclusion: We suggest DNAJA4 expression as a new biomarker candidate for breast cancer. Promoter hypomethylation could be an important epigenetic factor in the upregulation of DNAJA4 expression in breast cancer.","PeriodicalId":49599,"journal":{"name":"Revista Romana De Medicina De Laborator","volume":"168 1","pages":"369 - 378"},"PeriodicalIF":0.5,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86761349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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