Viruses-Basel最新文献_第7页

Acute Hepatitis C: Current Status and Future Perspectives. 急性丙型肝炎：现状与未来展望》。

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-11-06 DOI: 10.3390/v16111739

Massimo Fasano, Francesco Ieva, Marianna Ciarallo, Bruno Caccianotti, Teresa Antonia Santantonio

{"title":"Acute Hepatitis C: Current Status and Future Perspectives.","authors":"Massimo Fasano, Francesco Ieva, Marianna Ciarallo, Bruno Caccianotti, Teresa Antonia Santantonio","doi":"10.3390/v16111739","DOIUrl":"10.3390/v16111739","url":null,"abstract":"The hepatitis C virus (HCV) infection continues to represent a significant public health threat and is a leading cause of liver cirrhosis, liver cancer, and liver-related mortality. The World Health Organization (WHO) has set a goal for 2030: to eliminate HCV infection as a public health threat by reducing new HCV infections by 90% and mortality by 65%. The early phase of HCV infection represents a pivotal point in the evolution of hepatitis C. Despite a favourable course in the majority of patients, approximately 50-70% of individuals with recently acquired hepatitis C will develop a chronic infection, defined as the persistence of viremia for a period exceeding six months. The diagnosis and treatment of a recent HCV infection should facilitate engagement in multidisciplinary care, prevent the development and complications of chronic liver disease, and reduce ongoing transmission in key populations. Therefore, early treatment in the early phase of infection compared with deferring treatment until the chronic infection remains a valid approach in the era of direct antiviral agents (DAAs). This approach is supported by a cost-effectiveness analysis. The aim of this review is to synthesise the existing knowledge on the early phase of hepatitis C virus infection, with a particular focus on the current risk factors, natural history, therapeutic management, and future perspectives.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"16 11","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11599108/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of Oncolytic Measles Virus in Vero Cells: Impact of Culture Medium and Multiplicity of Infection. 在 Vero 细胞中产生麻疹病毒溶瘤：培养基和感染倍数的影响

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-11-06 DOI: 10.3390/v16111740

Dustin Eckhardt, Jana Mueller, Jonas Friedrich, Jan-P Klee, Irakli Sardlishvili, Lars E Walter, Stefanie Fey, Peter Czermak, Denise Salzig

{"title":"Production of Oncolytic Measles Virus in Vero Cells: Impact of Culture Medium and Multiplicity of Infection.","authors":"Dustin Eckhardt, Jana Mueller, Jonas Friedrich, Jan-P Klee, Irakli Sardlishvili, Lars E Walter, Stefanie Fey, Peter Czermak, Denise Salzig","doi":"10.3390/v16111740","DOIUrl":"10.3390/v16111740","url":null,"abstract":"Oncolytic measles virus (MeV) is a promising anti-cancer treatment. However, the production of high titers of infectious MeV (typically 107-109 TCID50 per dose) is challenging because the virus is unstable under typical production conditions. The objective of this study was to investigate how the multiplicity of infection (MOI) and different media-a serum-containing medium (SCM), a serum-free medium (SFM) and two chemically defined media (CDM)-affect MeV production. We infected Vero cells at MOIs of 0.02, 0.2 or 2 TCID50 cell-1 and the lowest MOI resulted in the largest number of infected cells towards the end of the production period. However, this did not equate to higher maximum MeV titers, which were similar for all the MOIs. The medium had a moderate effect, generating maximum titers of 0.89-2.17 × 106, 1.08-1.25 × 106 and 4.58-9.90 × 105 TCID50 mL-1 for the SCM, SFM and CDM, respectively. Infection at a low MOI often required longer process times to reach maximum yields. On the other hand, a high MOI requires a large amount of MeV stock. We would therefore recommend a mid-range MOI of 0.2 TCID50 cell-1 for MeV production. Our findings show that SCM, SFM and CDM are equally suitable for MeV production in terms of yield and process time. This will allow MeV production in serum-free conditions, addressing the safety risks and ethical concerns associated with the use of serum.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"16 11","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11599022/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological Characterization and Evaluation of the Therapeutic Value of Vibrio Phages 4141 and MJW Isolated from Clinical and Sewage Water Samples of Kolkata. 从加尔各答临床和污水样本中分离出的弧菌噬菌体 4141 和 MJW 的生物学特征和治疗价值评估。

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-11-06 DOI: 10.3390/v16111741

Sanjoy Biswas, Devendra Nath Tewari, Alok Kumar Chakrabarti, Shanta Dutta

{"title":"Biological Characterization and Evaluation of the Therapeutic Value of Vibrio Phages 4141 and MJW Isolated from Clinical and Sewage Water Samples of Kolkata.","authors":"Sanjoy Biswas, Devendra Nath Tewari, Alok Kumar Chakrabarti, Shanta Dutta","doi":"10.3390/v16111741","DOIUrl":"10.3390/v16111741","url":null,"abstract":"The growing prevalence of antimicrobial resistance (AMR) necessitates the development of new treatment methods to combat diseases like cholera. Lytic bacteriophages are viruses that specifically target and lyse bacteria upon infection, making them a possible treatment option for multi-drug-resistant pathogens. The current study investigated the potential role of bacteriophages isolated from clinical stool and sewage water samples in treating multi-drug-resistant Vibrio cholerae infection, finding that over 95% of the strains were susceptible. Whole-genome sequencing (WGS) analysis revealed that both Vibrio phage 4141 (4141) and Vibrio phage MJW (MJW) contain double-stranded DNA genomes consisting of 38,498 bp (43% GC) and 49,880 bp (42.5% GC) with 46 and 64 open reading frames (ORFs), respectively. Transmission electron microscope (TEM) and WGS analysis of Vibrio phage 4141 and Vibrio phage MJW validated that they are classified under the family Autographiviridae and Zobellviridae, respectively. Furthermore, both the phages showed highly significant biofilm degradation properties. The characterization of the phages and their strict host range, high spectrum of lytic ability, high efficiency of biofilm degradation, and close genetic similarity to the therapeutic phages indicates that these phages may be useful for therapeutic purposes for treating MDR V. cholerae infection in the future.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"16 11","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598976/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142734008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vesicular Stomatitis Virus Transmission Dynamics Within Its Endemic Range in Chiapas, Mexico. 墨西哥恰帕斯州水泡性口炎病毒流行区内的传播动态。

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-11-06 DOI: 10.3390/v16111742

Lawrence H Zhou, Federico Valdez, Irene Lopez Gonzalez, Willian Freysser Urbina, Ariadna Ocaña, Cristell Tapia, Armando Zambrano, Edilberto Hernandez Solis, Debra P C Peters, Chad E Mire, Roberto Navarro, Luis L Rodriguez, Kathryn A Hanley

{"title":"Vesicular Stomatitis Virus Transmission Dynamics Within Its Endemic Range in Chiapas, Mexico.","authors":"Lawrence H Zhou, Federico Valdez, Irene Lopez Gonzalez, Willian Freysser Urbina, Ariadna Ocaña, Cristell Tapia, Armando Zambrano, Edilberto Hernandez Solis, Debra P C Peters, Chad E Mire, Roberto Navarro, Luis L Rodriguez, Kathryn A Hanley","doi":"10.3390/v16111742","DOIUrl":"10.3390/v16111742","url":null,"abstract":"Vesicular stomatitis virus (VSV), comprising vesicular stomatitis New Jersey virus (VSNJV) and vesicular stomatitis Indiana virus (VSIV), emerges from its focus of endemic transmission in Southern Mexico to cause sporadic livestock epizootics in the Western United States. A dearth of information on the role of potential arthropod vectors in the endemic region hampers efforts to identify factors that enable endemicity and predict outbreaks. In a two-year, longitudinal study at five cattle ranches in Chiapas, Mexico, insect taxa implicated as VSV vectors (blackflies, sandflies, biting midges, and mosquitoes) were collected and screened for VSV RNA, livestock vesicular stomatitis (VS) cases were monitored, and serum samples were screened for neutralizing antibodies. VS cases were reported during the rainy (n = 20) and post-rainy (n = 2) seasons. Seroprevalence against VSNJV in adult cattle was very high (75-100% per ranch) compared with VSIV (0.6%, all ranches). All four potential vector taxa were sampled, and VSNJV RNA was detected in each of them (11% VSNJV-positive of 874 total pools), while VSIV RNA was only detected in four pools of mosquitoes. Our findings indicate that VSNJV is the dominant serotype across our sampling sites with a variety of potential insect vectors involved in its transmission throughout the year. Although no livestock cases were reported in Chiapas during the dry season, VSNJV was detected in insects during this period, suggesting that mechanisms other than transmission from livestock support VSV endemicity.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"16 11","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142734031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alteration of Gene Expression After Entecavir and Pegylated Interferon Therapy in HBV-Infected Chimeric Mouse Liver. 恩替卡韦和聚乙二醇干扰素治疗后 HBV 感染嵌合小鼠肝脏基因表达的改变

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-11-06 DOI: 10.3390/v16111743

Huarui Bao, Serami Murakami, Masataka Tsuge, Takuro Uchida, Shinsuke Uchikawa, Hatsue Fujino, Atsushi Ono, Eisuke Murakami, Tomokazu Kawaoka, Daiki Miki, Clair Nelson Hayes, Shiro Oka

{"title":"Alteration of Gene Expression After Entecavir and Pegylated Interferon Therapy in HBV-Infected Chimeric Mouse Liver.","authors":"Huarui Bao, Serami Murakami, Masataka Tsuge, Takuro Uchida, Shinsuke Uchikawa, Hatsue Fujino, Atsushi Ono, Eisuke Murakami, Tomokazu Kawaoka, Daiki Miki, Clair Nelson Hayes, Shiro Oka","doi":"10.3390/v16111743","DOIUrl":"10.3390/v16111743","url":null,"abstract":"Cross-sectional analyses using liver tissue from chronic hepatitis B patients make it difficult to exclude the influence of host immune responses. In this study, we performed next-generation sequencing using the livers of hepatitis B virus (HBV)-infected uPA/SCID mice with humanized livers before and after antiviral therapy (AVT) with entecavir and pegylated interferon, and then performed a comparative transcriptome analysis of gene expression alteration. After HBV infection, the expression of genes involved in multiple pathways was significantly altered in the HBV-infected livers. After AVT, the levels of 37 out of 89 genes downregulated by HBV infection were restored, and 54 of 157 genes upregulated by HBV infection were suppressed. Interestingly, genes associated with hypoxia and KRAS signaling were included among the 54 genes upregulated by HBV infection and downregulated by AVT. Several genes associated with cell growth or carcinogenesis via hypoxia and KRAS signaling were significantly downregulated by AVT, with a potential application for the suppression of hepato-carcinogenesis.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"16 11","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyanophage Engineering for Algal Blooms Control. 控制藻华的噬蓝藻工程。

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-11-06 DOI: 10.3390/v16111745

Yujing Guo, Xiaoxiao Dong, Huiying Li, Yigang Tong, Zihe Liu, Jin Jin

{"title":"Cyanophage Engineering for Algal Blooms Control.","authors":"Yujing Guo, Xiaoxiao Dong, Huiying Li, Yigang Tong, Zihe Liu, Jin Jin","doi":"10.3390/v16111745","DOIUrl":"10.3390/v16111745","url":null,"abstract":"Cyanobacteria represent a prevalent category of photosynthetic autotrophs capable of generating deleterious algal blooms, commonly known as cyanobacteria harmful algal blooms (cyanoHABs). These blooms often produce cyanotoxins, which pose risks to public health and ecosystems by contaminating surface waters and drinking water sources. Traditional treatment methods have limited effectiveness. Therefore, there is an urgent need for a new approach to effectively manage cyanoHABs. One promising approach is the use of cyanophages, which are viruses that specifically target cyanobacteria. Cyanophages serve as an effective biological control method for reducing cyanoHABs in aquatic systems. By engineering cyanophages, it is possible to develop a highly specific control strategy that minimally impacts non-target species and their propagation in the environment. This review explores the potential application of cyanophages as a strategy for controlling cyanoHABs. It includes the identification and isolation of broad-spectrum and novel cyanophages, with a specific focus on freshwater Microcystis cyanophages, highlighting their broad spectrum and high efficiency. Additionally, recent advancements in cyanophage engineering are discussed, including genome modification, functional gene identification, and the construction of artificial cyanophages. Furthermore, the current state of application is addressed. Cyanophage is a promising control strategy for effectively managing cyanoHABs in aquatic environments.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"16 11","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598953/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142734207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mpox Resurgence: A Multifaceted Analysis for Global Preparedness. 麻疹病毒卷土重来：全球备战的多层面分析。

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-11-05 DOI: 10.3390/v16111737

Fatouma Mohamed Abdoul-Latif, Ayoub Ainane, Houda Mohamed, Ali Merito Ali, Ibrahim Houmed Aboubaker, Pannaga Pavan Jutur, Tarik Ainane

引用次数: 0

Correction: Tajima et al. Amino Acids at Positions 156 and 332 in the E Protein of the West Nile Virus Subtype Kunjin Virus Classical Strain OR393 Are Involved in Plaque Size, Growth, and Pathogenicity in Mice. Viruses 2024, 16, 1237. 更正：Tajima et al. 西尼罗河病毒亚型昆金病毒经典株 OR393 E 蛋白中 156 和 332 位氨基酸参与小鼠斑块大小、生长和致病性。Viruses 2024, 16, 1237.

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-11-05 DOI: 10.3390/v16111736

Shigeru Tajima, Hideki Ebihara, Chang-Kweng Lim

引用次数: 0

Impact of Different Foot and Mouth Disease Vaccine Schemes in Cross-Neutralization Against Heterologous Serotype O Strains in Cattle. 不同口蹄疫疫苗方案对牛异源血清型 O 株交叉中和的影响

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-11-04 DOI: 10.3390/v16111732

María Cruz Miraglia, Melanie Barrios-Benito, Sabrina Galdo-Novo, Danilo Bucafusco, Ana Taffarel, Alejandra Victoria Capozzo, Manuel Victor Borca, Daniel Mariano Pérez-Filgueira

{"title":"Impact of Different Foot and Mouth Disease Vaccine Schemes in Cross-Neutralization Against Heterologous Serotype O Strains in Cattle.","authors":"María Cruz Miraglia, Melanie Barrios-Benito, Sabrina Galdo-Novo, Danilo Bucafusco, Ana Taffarel, Alejandra Victoria Capozzo, Manuel Victor Borca, Daniel Mariano Pérez-Filgueira","doi":"10.3390/v16111732","DOIUrl":"10.3390/v16111732","url":null,"abstract":"The high antigenic variability of the foot-and-mouth disease virus (FMDV) represents a challenge for developing prophylactic strategies, stressing the need for research into vaccines offering broad protection against a range of virus strains. Here, the heterotypic cross-reaction using different vaccine schemes against serotype O strains was studied, evaluating the impact of revaccination, antigen dose, and incorporation of additional FMDV serotypes. Naïve cattle were immunized with seven distinct FMDV vaccines, receiving three doses of the same formulation at 0, 28, and 56 days post-primary vaccination (dpv). Serum samples were collected up to 70 dpv and tested by a virus-neutralizing test against serotype O strains from a South American lineage and two strains representative of two Asian lineages. Our results showed that vaccines containing the ME-SA topotype O1/Campos strain developed cross-neutralizing responses against the two Asian viruses after the first vaccination. In contrast, significant heterotypic neutralizing antibody titers against the homologous topotype strain were only found after the second vaccination, indicating that the phylogenic relationship may differ from the antigenic profiles for these two viruses. The amount of the O1/Campos strain and the revaccination were essential factors for neutralization against the homologous- and heterologous-type O FMDV viruses. The strain composition of the vaccine was only relevant for cross-neutralization against one of the Asian strains, suggesting potential intra-serotypic divergences for this pattern.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"16 11","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11599131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142734009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dimerization of Rabies Virus Phosphoprotein and Phosphorylation of Its Nucleoprotein Enhance Their Binding Affinity. 狂犬病毒磷蛋白的二聚化及其核蛋白的磷酸化增强了它们的结合亲和力

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-11-04 DOI: 10.3390/v16111735

Euripedes de Almeida Ribeiro, Cédric Leyrat, Francine C A Gérard, Marc Jamin

{"title":"Dimerization of Rabies Virus Phosphoprotein and Phosphorylation of Its Nucleoprotein Enhance Their Binding Affinity.","authors":"Euripedes de Almeida Ribeiro, Cédric Leyrat, Francine C A Gérard, Marc Jamin","doi":"10.3390/v16111735","DOIUrl":"10.3390/v16111735","url":null,"abstract":"The dynamic interplay between a multimeric phosphoprotein (P) and polymeric nucleoprotein (N) in complex with the viral RNA is at the heart of the functioning of the RNA-synthesizing machine of negative-sense RNA viruses of the order Mononegavirales. P multimerization and N phosphorylation are often cited as key factors in regulating these interactions, but a detailed understanding of the molecular mechanisms is not yet available. Working with recombinant rabies virus (RABV) N and P proteins and using mainly surface plasmon resonance, we measured the binding interactions of full-length P dimers and of two monomeric fragments of either circular or linear N-RNA complexes, and we analyzed the equilibrium binding isotherms using different models. We found that RABV P binds with nanomolar affinity to both circular and linear N-RNA complexes and that the dimerization of P protein enhances the binding affinity by 15-30-fold as compared to the monomeric fragments, but less than expected for a bivalent ligand, in which the binding domains are connected by a flexible linker. We also showed that the phosphorylation of N at Ser389 creates high-affinity sites on the polymeric N-RNA complex that enhance the binding affinity of P by a factor of about 360.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"16 11","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11599015/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142734237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0