Progress in Molecular Biology and Translational Science最新文献_第6页

Phages for treatment Pseudomonas aeruginosa infection. 治疗铜绿假单胞菌感染的噬菌体。

3区生物学

Progress in Molecular Biology and Translational Science Pub Date : 2023-01-01 Epub Date: 2023-04-07 DOI: 10.1016/bs.pmbts.2023.03.014

Salsabil Makky, Fatma Abdelrahman, Nouran Rezk, Maheswaran Easwaran, Ayman El-Shibiny

引用次数: 0

Phage for treatment of Vibrio cholerae infection. 用于治疗霍乱弧菌感染的噬菌体。

3区生物学

Progress in Molecular Biology and Translational Science Pub Date : 2023-01-01 Epub Date: 2023-04-17 DOI: 10.1016/bs.pmbts.2023.03.021

Milky Mittal, Surbhi Tripathi, Ashok Saini, Indra Mani

{"title":"Phage for treatment of Vibrio cholerae infection.","authors":"Milky Mittal, Surbhi Tripathi, Ashok Saini, Indra Mani","doi":"10.1016/bs.pmbts.2023.03.021","DOIUrl":"10.1016/bs.pmbts.2023.03.021","url":null,"abstract":"Bacteriophages (or \"phages\") are ubiquitous and the amplest biological entities on our planet. It is a natural enemy of bacteria. Cholera is one of the most known diseases to cause multiple pandemics around the world, killing millions of people. The pathogen of cholera is Vibrio species. Up until the emergence of multidrug resistance, preventive therapeutics like antibiotics were the most effective means of battling bacteria. Globally, one of the most significant challenges in treating microbial infections is the development of drug-resistant strains. Based on their antibacterial properties and unique characteristics, phages are being comprehensively evaluated taxonomically. Moreover, phage-based vaccination is evolving as one of the most encouraging preventive approaches. Due to this, its related research got remarkable recognition. However, due to the rapid emergence of bacterial resistance to antibiotics, the use of phages (phage therapy) could be a major motive for research because the most promising solution lies in bacteriophages. This chapter briefly highlights the promising use of bacteriophages to combat Vibrio-related infectious diseases.","PeriodicalId":49280,"journal":{"name":"Progress in Molecular Biology and Translational Science","volume":"201 ","pages":"21-39"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41156171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phage design and directed evolution to evolve phage for therapy. 噬菌体设计和定向进化，进化出用于治疗的噬菌体。

3区生物学

Progress in Molecular Biology and Translational Science Pub Date : 2023-01-01 Epub Date: 2023-06-19 DOI: 10.1016/bs.pmbts.2023.03.019

Priyancka Arora, Avni Jain, Ajay Kumar

{"title":"Phage design and directed evolution to evolve phage for therapy.","authors":"Priyancka Arora, Avni Jain, Ajay Kumar","doi":"10.1016/bs.pmbts.2023.03.019","DOIUrl":"https://doi.org/10.1016/bs.pmbts.2023.03.019","url":null,"abstract":"Phage therapy or Phage treatment is the use of bacteriolysing phage in treating bacterial infections by using the viruses that infects and kills bacteria. This technique has been studied and practiced very long ago, but with the advent of antibiotics, it has been neglected. This foregone technique is now witnessing a revival due to development of bacterial resistance. Nowadays, with the awareness of genetic sequence of organisms, it is required that informed choices of phages have to be made for the most efficacious results. Furthermore, phages with the evolving genes are taken into consideration for the subsequent improvement in treating the patients for bacterial diseases. In addition, direct evolution methods are increasingly developing, since these are capable of creating new biological molecules having changed or unique activities, such as, improved target specificity, evolution of novel proteins with new catalytic properties or creation of nucleic acids that are capable of recognizing required pathogenic bacteria. This system is incorporates continuous evolution such as protein or genes are put under continuous evolution by providing continuous mutagenesis with least human intervention. Although, this system providing continuous directed evolution is very effective, it imposes some challenges due to requirement of heavy investment of time and resources. This chapter focuses on development of phage as a therapeutic agent against various bacteria causing diseases and it improvement using direct evolution of proteins and nucleic acids such that they target specific organisms.","PeriodicalId":49280,"journal":{"name":"Progress in Molecular Biology and Translational Science","volume":"200 ","pages":"103-126"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41159811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phages for treatment of Staphylococcus aureus infection. 治疗金黄色葡萄球菌感染的噬菌体。

3区生物学

Progress in Molecular Biology and Translational Science Pub Date : 2023-01-01 Epub Date: 2023-05-03 DOI: 10.1016/bs.pmbts.2023.03.027

Safia Samir

{"title":"Phages for treatment of Staphylococcus aureus infection.","authors":"Safia Samir","doi":"10.1016/bs.pmbts.2023.03.027","DOIUrl":"10.1016/bs.pmbts.2023.03.027","url":null,"abstract":"Combating multi-drug resistant bacterial infections should be a universal urgency. The gram- positive Staphylococcus aureus (S. aureus) bacteria are generally harmless; healthy people frequently have them on their skin and nose. These bacteria, for the most part, produce no difficulties or only minor skin diseases. Antibiotics and cleansing of the affected region are usually the treatments of choice. S. aureus can become virulent causing serious infections that may lead to pustules to sepsis or death. Normally, it is thought that antibiotics may solve problems concerning bacterial infection; but unfortunately, Staphylococci have evolved mechanisms to resist drugs. Methicillin-Resistant Staphylococcus aureus (MRSA); both in hospitals and in the community, infections are evolving into dangerous pathogens. Health care practitioners may need to use antibiotics with more adverse effects to treat antibiotic-resistant S. aureus infections. Amid existing efforts to resolve this problem, phage therapy proposes a hopeful alternate to face Staphylococcal infections. When the majority of antibiotics have failed to treat infections caused by multidrug-resistant bacteria, such as methicillin- and vancomycin-resistant S. aureus, phage therapy may be an option. Here, we appraise the potential efficacy, current knowledge on bacteriophages for S. aureus, experimental research and information on their clinical application, and limitations of phage therapy for S. aureus infections.","PeriodicalId":49280,"journal":{"name":"Progress in Molecular Biology and Translational Science","volume":"200 ","pages":"275-302"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41177339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the potential of phages: Innovative approaches to harnessing bacteriophages as diagnostic tools for human diseases. 释放噬菌体的潜力：利用噬菌体作为人类疾病诊断工具的创新方法。

3区生物学

Progress in Molecular Biology and Translational Science Pub Date : 2023-01-01 Epub Date: 2023-04-20 DOI: 10.1016/bs.pmbts.2023.03.025

Vishnu Kirthi Arivarasan

{"title":"Unlocking the potential of phages: Innovative approaches to harnessing bacteriophages as diagnostic tools for human diseases.","authors":"Vishnu Kirthi Arivarasan","doi":"10.1016/bs.pmbts.2023.03.025","DOIUrl":"https://doi.org/10.1016/bs.pmbts.2023.03.025","url":null,"abstract":"Phages, viruses that infect bacteria, have been explored as promising tools for the detection of human disease. By leveraging the specificity of phages for their bacterial hosts, phage-based diagnostic tools can rapidly and accurately detect bacterial infections in clinical samples. In recent years, advances in genetic engineering and biotechnology have enabled the development of more sophisticated phage-based diagnostic tools, including those that express reporter genes or enzymes, or target specific virulence factors or antibiotic resistance genes. However, despite these advancements, there are still challenges and limitations to the use of phage-based diagnostic tools, including concerns over phage safety and efficacy. This review aims to provide a comprehensive overview of the current state of phage-based diagnostic tools, including their advantages, limitations, and potential for future development. By addressing these issues, we hope to contribute to the ongoing efforts to develop safe and effective phage-based diagnostic tools for the detection of human disease.","PeriodicalId":49280,"journal":{"name":"Progress in Molecular Biology and Translational Science","volume":"201 ","pages":"133-149"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41150565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phage for drug delivery vehicles. 用于药物输送车辆的噬菌体。

3区生物学

Progress in Molecular Biology and Translational Science Pub Date : 2023-01-01 Epub Date: 2023-06-17 DOI: 10.1016/bs.pmbts.2023.04.008

Mohit Kumar, Piyush Parkhey, Santosh Kumar Mishra, Prabir Kumar Paul, Avinash Singh, Vijai Singh

引用次数: 0

Exploring the potential of phage and their applications. 探索噬菌体的潜力及其应用。

3区生物学

Progress in Molecular Biology and Translational Science Pub Date : 2023-01-01 Epub Date: 2023-05-11 DOI: 10.1016/bs.pmbts.2023.04.001

Khushal Khambhati, Gargi Bhattacharjee, Nisarg Gohil, Rupesh Maurya, Vijai Singh

{"title":"Exploring the potential of phage and their applications.","authors":"Khushal Khambhati, Gargi Bhattacharjee, Nisarg Gohil, Rupesh Maurya, Vijai Singh","doi":"10.1016/bs.pmbts.2023.04.001","DOIUrl":"https://doi.org/10.1016/bs.pmbts.2023.04.001","url":null,"abstract":"Antibiotic resistant microorganisms are significantly increasing due to horizontal gene transfer, mutation and overdose of antibiotics leading to serious health conditions globally. Several multidrug resistant microorganisms have shown resistance to even the last line of antibiotics making it very difficult to treat them. Besides using antibiotics, an alternative approach to treat such resistant bacterial pathogens through the use of bacteriophage (phage) was used in the early 1900s which however declined and vanished after the discovery of antibiotics. In recent times, phage has emerged and gained interest as an alternative approach to antibiotics to treat MDR pathogens. Phage can self-replicate by utilizing cellular machinery of bacterial host by following lytic and lysogenic life cycles and therefore suitable for rapid regeneration. Application of phage for detection of bacterial pathogens, elimination of bacteria, agents for controlling food spoilage, treating human disease and several others entitles phage as a futuristic antibacterial armamentarium.","PeriodicalId":49280,"journal":{"name":"Progress in Molecular Biology and Translational Science","volume":"200 ","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41155151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, screening and characterization of phage. 噬菌体的分离、筛选和鉴定。

3区生物学

Progress in Molecular Biology and Translational Science Pub Date : 2023-01-01 Epub Date: 2023-04-07 DOI: 10.1016/bs.pmbts.2023.03.008

Bishoy Maher Zaki, Amira A Mohamed, Alyaa Dawoud, Kareem Essam, Zainab K Hammouda, Abdallah S Abdelsattar, Ayman El-Shibiny

引用次数: 1

Phage and phage cocktails formulations. 噬菌体和噬菌体鸡尾酒配方。

3区生物学

Progress in Molecular Biology and Translational Science Pub Date : 2023-01-01 Epub Date: 2023-05-05 DOI: 10.1016/bs.pmbts.2023.04.007

Indra Mani

引用次数: 0

Phage engineering for development of diagnostic tools. 用于开发诊断工具的噬菌体工程。

3区生物学

Progress in Molecular Biology and Translational Science Pub Date : 2023-01-01 Epub Date: 2023-06-26 DOI: 10.1016/bs.pmbts.2023.04.004

Subha Sankar Paul

{"title":"Phage engineering for development of diagnostic tools.","authors":"Subha Sankar Paul","doi":"10.1016/bs.pmbts.2023.04.004","DOIUrl":"https://doi.org/10.1016/bs.pmbts.2023.04.004","url":null,"abstract":"The bacteriophages rely on the host cell to provide energy and resources for their own replication. Antibody-based diagnostic tests rely on the antibody and the biomarker interactions. Since, most of these diagnostic tools employ the use of antibodies; hence, they require intensive storage protocols at cold conditions and incur high time and capital cost due to their production and purification process. Phage-based diagnostics can overcome this limitation. Bacteriophages, have been used as emerging tools for the detection of various pathogens. Rapid phage-mediated detection assays have become commercial diagnostic tools. Conventional method and new cloning approaches have been followed to specifically detect a disease- causing microbial strains. This review discusses use of Phage typing as diagnostic tools, phage-based detection methods, and their usage for signal amplification. Design rules for reporter phage engineering are also discussed followed by different engineering platforms for phage genome editing. We also discuss recent examples of how phage research is influencing the recent advances in the development of phage-based diagnostics for ultra-sensitive detection of various bio-species, outlining the advantages and limitations of detection technology of phage-based assays.","PeriodicalId":49280,"journal":{"name":"Progress in Molecular Biology and Translational Science","volume":"200 ","pages":"91-101"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41178986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0