Indian Journal of Hematology and Blood Transfusion最新文献

{"title":"Assessment of Serum Autophagy Related Protein Beclin-1 in Egyptian Adult Beta Thalassemia Patients.","authors":"Haydi S Mohamed, Amal M El-Afifi, Nermeen A Nabih, Esraa M Mohamed, Mostafa K El-Razzaz","doi":"10.1007/s12288-024-01865-0","DOIUrl":"10.1007/s12288-024-01865-0","url":null,"abstract":"<p><p>Beclin-1 has a significant role in autophagy process (Macro-autophagy). It functions through interaction with autophagy-related genes (Atgs) and other protein molecules during the process of autophagy.Here we assessed the level of serum autophagy related protein Beclin-1 in Egyptian adult beta thalassemia patients and studied its relation to ineffective erythropoiesis, transfusion requirements, iron overload.This case control study included 50 Egyptian adult beta thalassemia patients aged 18 years or more including transfusion dependent and non-transfusion dependent patients, and 25 healthy controls. The patients were selected from the Hematology Department of Ain Shams University Hospitals, Cairo, Egypt.The median (IQR) Beclin-1 level was higher in patients group in comparison to control group with highly significant statistical difference {15ng/ml (13-22) Vs 2.5ng/ml (2-3), p value < 0.001}. We found a statistically significant positive correlation between Beclin-1 level and serum ferritin (<i>r</i> = 0.297, p value 0.036), a negative correlation between Beclin-1 level and EF% (<i>r</i>=-0.379, p value 0.007), this reflects that increased Beclin-1 was associated with iron overload especially cardiac iron overload. Statistically positive correlations between Beclin-1 and liver iron concentration, transfusion/year, serum ferritin, serum iron and transferrin saturation were also found.Elevated level of Beclin-1 in adult beta Thalassemia patients correlated with cardiac and liver iron overload.</p>","PeriodicalId":49188,"journal":{"name":"Indian Journal of Hematology and Blood Transfusion","volume":"41 3","pages":"545-550"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12267698/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144676213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Expressor Lymphoma Prevalence in High-Grade Large B-cell Lymphomas: An Observational Analysis From a Tertiary Care Centre.","authors":"Snigdha Roy, Md Ali Osama, Nitin Gupta, Sonia Badwal, Seema Rao","doi":"10.1007/s12288-024-01910-y","DOIUrl":"https://doi.org/10.1007/s12288-024-01910-y","url":null,"abstract":"<p><p>Double or Triple Hit Lymphomas (DHL/THL) represent a subset of aggressive B-cell lymphomas with markedly poorer outcomes and survival rates compared to Diffuse Large B-cell Lymphomas, NOS (DLBCL, NOS). Identifying the Hit status in High-grade large B-cell lymphoma (HGBL)/ Diffuse Large B-cell Lymphomas (used synonymously henceforth) can aid in identifying cases with aggressive clinical course that may benefit from more intensive chemotherapy. Utilizing immunohistochemistry (IHC) to detect Expressor Lymphomas (EL) provides a simpler and cost-effective means to screen DLBCL cases for further molecular studies aimed at identifying potential Hit status. Our investigation aimed to assess the occurrence of Expressor Lymphoma cases in the Indian population and compare clinical (age, gender, site of tumor and B-symptoms) and pathological parameters {Ki67 proliferation index and Germinal Centre/Non-Germinal Centre (GC/Non-GC) phenotype} between Expressor and Non-Expressor Lymphomas (NEL). The present study included 131 patients diagnosed with HGBL. Men comprised 62.6% of patients, with 58% aged 60 years or younger. Clinical data on the occurrence of B-symptoms was accessible for 99 patients, with only 35 of them displaying such symptoms. Serum LDH levels were measured in 34 of the 131 total cases, and 30 of these showed elevated levels. Nodal disease was present in 26% of cases, while the remaining had extranodal involvement. The GC phenotype was observed in 58.8% of cases. Out of 131 cases, 22.2% were EL, while 77.8% were NEL. ELs exhibited a higher mean Ki67 value (<i>p</i> = 0.025) compared to NEL cases, indicating increased proliferation. No significant correlation was found between EL and NEL with respect to age, gender, tumor site, B-symptoms, LDH levels, or GC/non-GC phenotype. In the present study, out of a total of 131 cases, 29 were classified as EL (22.1%), while 102 cases (77.8%) were classified as NEL. It is recommended to perform IHC to detect expressor status in all cases of DLBCL, as a significant percentage of them may turn out to be ELs, indicating a potentially aggressive clinical course that warrants therapy intensification. Moreover, this IHC panel may serve as a screening tool to identify cases requiring further molecular studies for potential Hit status detection.</p>","PeriodicalId":49188,"journal":{"name":"Indian Journal of Hematology and Blood Transfusion","volume":"41 3","pages":"519-529"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12267721/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144676212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anaemia in First Trimester: Proportion, Selected Causes and Associated Factors among First Trimester Pregnant Women Attending Antenatal Clinics in Colombo District, Sri Lanka.","authors":"W R Heiyanthuduwa, Khbp Fernandopulle, Cse Goonewardena, Klmd Seneviwickrama","doi":"10.1007/s12288-024-01840-9","DOIUrl":"https://doi.org/10.1007/s12288-024-01840-9","url":null,"abstract":"<p><strong>Purpose: </strong>To determine the proportion of anaemia and selected causes during the first trimester among pregnant women in selected Medical Officer of Health areas in Colombo District, Sri Lanka.</p><p><strong>Methods: </strong>A descriptive cross-sectional study was conducted among 700 first trimester pregnant women attending antenatal clinics using a pretested interviewer administered questionnaire. Blood samples were collected for relevant full blood count and blood picture. Severity of anaemia was categorised using WHO cutoff values. Data was analysed using SPSS version 26.0. Logistic regression was used to identify associated factors for anaemia. Level of significance was considered as <i>p</i> < 0.05.</p><p><strong>Results: </strong>The proportion of anaemia was 13.6% (mild-64.2%, moderate-34.7%, and severe-1.1%). The commonest cause of anaemia was iron deficiency (90.5%) followed by thalassemia trait 6.3%, vitamin B12/folate deficiency 1.1%, and other 2.2%.</p><p><strong>Conclusion: </strong>Proportion of first trimester anaemia was higher than the reported prevalence in other regional studies such as in India (7.7%) and Bangladesh (10.4%). The most common cause of anaemia was iron deficiency. Findings highlight the need for early screening and intervention for anaemia among pregnant women.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12288-024-01840-9.</p>","PeriodicalId":49188,"journal":{"name":"Indian Journal of Hematology and Blood Transfusion","volume":"41 3","pages":"670-673"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12267791/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144676281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetics of B-Cell Acute Lymphoblastic Leukemia (B-ALL): Recent Updates and Indian Perspective.","authors":"Sanjeev Kumar Gupta, Gadha K Leons","doi":"10.1007/s12288-025-01988-y","DOIUrl":"https://doi.org/10.1007/s12288-025-01988-y","url":null,"abstract":"<p><p>B-cell acute lymphoblastic leukemia (B-ALL) is the most common hematological malignancy of childhood. The initial risk-stratification includes the genetic evaluation for common recurrent translocations and aneuploidies in B-ALL using karyotyping, fluorescence in-situ hybridization (FISH), reverse transcription polymerase chain reaction (RT-PCR), etc. However, many cases remain genetically unclassified and are labeled as B-other ALL. Recent advances in the high throughput genomics have provided new insights and many distinct genetic subtypes have been identified in B-ALL. These new subtypes have been incorporated in the latest World Health Organization (WHO)-HAEM5 classification of haematolymphoid tumors and the International Consensus Classification. Besides the driver mutations, there is also a role of secondary genetic events like copy number alterations (CNAs) in the pathogenesis of B-ALL as well as for better risk-stratification. New molecular genetic risk evaluation like Moorman's criteria, IKZF1plus and MRplus scores have been recently validated which can risk-stratify B-ALL patients at baseline based on the CNA profile. The new diagnostic armamentarium now also includes flow-based ploidy analysis, cytokine receptor like factor 2 (CRLF2/TSLPR) overexpression screening on flow cytometry, multiplex ligation dependent probe amplification (MLPA) for CNAs, targeted RNA sequencing and whole transcriptome sequencing for detection of known and novel fusions. The gene expression profiling data can also be assessed with the machine learning based genetic classifiers for prediction of the newly identified subtypes of B-ALL. This review attempts to provide updates about the latest developments in the field of B-ALL genetics with inputs of Indian data, wherever available.</p>","PeriodicalId":49188,"journal":{"name":"Indian Journal of Hematology and Blood Transfusion","volume":"41 3","pages":"460-477"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12267770/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144676300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of Redox Metabolism, CD147, and CD47 Expression with the Maturation of Hematopoietic Stem Cells in Bone Marrow.","authors":"Nitin Bhardwaj, Ashutosh Singh, Ram Babu, Mohammad Z Ahmed","doi":"10.1007/s12288-024-01911-x","DOIUrl":"https://doi.org/10.1007/s12288-024-01911-x","url":null,"abstract":"<p><p>Haematopoiesis is a continuous process that involves the commitment of pluripotent hematopoietic stem cells (HSCs) into multipotent progenitors (MPPs) and differentiation into functional blood cells. The current study aimed to investigate the prevalence of various HSC progenitors in the bone marrow (BM) as well as alterations in the production of reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and surface expression level of basigin (CD147) and integrin-associated protein (CD47) receptors on various HSC lineages. Different HSC progenitors (GMP, CMP, MEP, MPP, ST-HSC, and LT-HSC) cells were recognised based on the surface expression of CD117/Sca-1/CD16/32/CD34 and CD117/Sca-1/CD135/CD34 receptors by flow cytometric analysis. Our results suggest that among Sca-1^- cells, the proportion of MPP cells was significantly higher than STHSC and LTHSC. Among Sca1⁺ cells, the GMP cells were more prevalent, approximately three times more than CMP, while MEP cells were least in proportion. The HSC maturation leads to a significant change in metabolism and surface receptor expression. The LTHSC and STHSC have similar ROS levels, but it was 7 times greater on MPP. The ROS level also increased from CMP to GMP transition (34% increase), but it was reduced significantly on MEP. MMP was significantly higher on MPP and GMP as compared to LTHSC and STHSC, and CMP and MEP, respectively. The surface expression of CD147 was increased significantly from LTHSC/STHSC to MPP and CMP to GMP transition. CD47 receptor expression was also increased during the maturation of HSC. It was almost similar on LTHSC and STHSC but increased on MPP. Similarly, CMP and MEP cells have equivalent CD47 expression but are upregulated on GMP. Overall, HSC maturation involves a series of metabolic changes, which regulate HSC differentiation into progenitor cells in the BM.</p>","PeriodicalId":49188,"journal":{"name":"Indian Journal of Hematology and Blood Transfusion","volume":"41 3","pages":"629-639"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12267754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144676305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blastic Plasmacytoid Dendritic Cell Neoplasm Mimicking T-Cell Acute Lymphoblastic Leukemia: A Diagnostic Challenge.","authors":"O K Neshahel, Ann Thomas, K Binshad Ameer, P S Sreedharan","doi":"10.1007/s12288-024-01912-w","DOIUrl":"https://doi.org/10.1007/s12288-024-01912-w","url":null,"abstract":"","PeriodicalId":49188,"journal":{"name":"Indian Journal of Hematology and Blood Transfusion","volume":"41 3","pages":"732-735"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12267696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144676215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection Discrepancy: Capillary Zone Electrophoresis Misses Hemoglobin Malmo Identified by Cation Exchange-High Performance Liquid Chromatography.","authors":"Ekta Jajodia, Ankit Raiyani, Neeraj Arora, Mohan Galande, Ankit Jitani, Avinash Panchal","doi":"10.1007/s12288-024-01870-3","DOIUrl":"https://doi.org/10.1007/s12288-024-01870-3","url":null,"abstract":"","PeriodicalId":49188,"journal":{"name":"Indian Journal of Hematology and Blood Transfusion","volume":"41 3","pages":"714-716"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12267724/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144676220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Release of Platelet DAMPs is Regulated by NINJ1-Mediated Plasma Membrane Rupture.","authors":"Xiaoli Zhou, Guang Xin, Chengyu Wan, Fan Li, Yilan Wang, Kun Zhang, Xiuxian Yu, Shiyi Li, Wen Huang","doi":"10.1007/s12288-024-01876-x","DOIUrl":"https://doi.org/10.1007/s12288-024-01876-x","url":null,"abstract":"<p><p>Damage-associated molecular patterns (DAMPs) can cause platelet activation and coagulation system dysfunction. It is often released by broken cell membranes after cell death. The oligomerization of ninjurin-1 (NINJ1) is closely associated with platelet plasma membrane rupture, but whether it actually prevents the release of platelet DAMPs is unclear. The aim of this study was to evaluate whether NINJ1 deficiency can maintain the integrity of dead platelet cell membranes. The release of extracellular DAMPs was detected by ELISA, and the release of platelet particles was detected by flow cytometry. In order to further evaluate the potential effect of inhibiting platelet DAMPs release on the regulation of inflammatory diseases, we extracted platelet supernatant after agonist-induced death and co-culture with endothelial cells. It was confirmed that the inflammatory secretion of endothelial cells induced by NINJ1^-/- platelet supernatant was significantly reduced. These data suggest that NINJ1 deletion restriction inhibits platelet DAMPs release, suggesting a potential target for the treatment of related diseases.</p>","PeriodicalId":49188,"journal":{"name":"Indian Journal of Hematology and Blood Transfusion","volume":"41 3","pages":"698-703"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12267745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144676292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors Influencing the Severity of Jaundice in Direct Antiglobulin (Coomb's) Test Positive Neonates.","authors":"T Rabiyathul Sanofar Nisha, Nidhya Ganesan, Prasanna N Kumar, Ramesh Srinivasan","doi":"10.1007/s12288-024-01871-2","DOIUrl":"10.1007/s12288-024-01871-2","url":null,"abstract":"<p><strong>Purpose: </strong>Direct Antiglobulin Test (DAT) is a screening method used to identify Hemolytic Diseases of Newborn (HDN). Risk of neonatal jaundice for infants with positive DAT is four times higher than those with negative DAT. However, clinical guidelines regarding the risk factors amongst DAT positive neonates requiring phototherapy are not clear. This study aimed to assess the risk factors that influence the severity of jaundice among DAT positive neonates and its impact on treatment.</p><p><strong>Methods: </strong>A one-year retrospective observational study (2022-2023) which included all DAT- positive newborn babies reported in that period.</p><p><strong>Results: </strong>In our study period 134 babies had jaundice and were given phototherapy (cases), 19 did not have jaundice (controls). The two groups were compared for strength of DAT, neonatal risk factors such as asphyxia, sepsis, exclusive breast feeding, excess weight loss and maternal risk factors including Indirect Antiglobulin Test (IAT) status. A statistically significant increase in the bilirubin levels from day 1 to day 3 (<i>p</i> value of < 0.05) was observed. Multivariate regression analysis performed showed significant association between the risk factors and neonatal serum bilirubin levels.</p><p><strong>Conclusion: </strong>In the department of Neonatology, a positive DAT is an indicator to perform bilirubin levels in the newborn and subsequently monitor these levels. DAT also helps to diagnose if blood group mismatches are the reason for the hyperbilirubinemia. This is essential for the management of neonatal jaundice. However, in addition to positive DAT, the presence of other neonatal and maternal risk factors also influences the severity of neonatal hyperbilirubinemia.</p>","PeriodicalId":49188,"journal":{"name":"Indian Journal of Hematology and Blood Transfusion","volume":"41 3","pages":"613-621"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12267763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144676299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between Gravidity and Anemia in Tabari Cohort Population: A Case-Control Study.","authors":"Saber Palar, Mohammad Eslami, Akbar Hedayatizadeh-Omran, Mahmood Moosazadeh, Motahareh Kheradmand","doi":"10.1007/s12288-024-01903-x","DOIUrl":"https://doi.org/10.1007/s12288-024-01903-x","url":null,"abstract":"<p><p>Anemia is a global public health concern. It is more prevalent in women and previous studies have mostly been limited to pregnant women. Therefore, this study aimed to investigate the association between gravidity and anemia in a subset of women enrolled in the Tabari Cohort study. This case-control study included 1676 women aged 35-70 years old with at least one pregnancy. The case group consisted of 838 women who were anemic by definition, and were included using the census method. The control group (838 women) was randomly selected from the rest of the study population based on age. In this study, a subset of data from the enrolment phase of the Tabari cohort was analyzed. Statistical analysis was performed using multivariate logistic regression, chi-square test, and independent sample t-test in SPSS. The mean gravidity in the case and control group was 3.23 ± 1.74 and 3.53 ± 1.98, respectively (<i>P</i> = 0.001).In the case group, the frequencies of 1, 2, 3, 4, and ≥ 5 gravidity was 10.3%, 30.2%, 25.8%, 15.5%, and 18.3%, respectively; in the control group, the frequencies were 8.2%, 27.8%, 24.7%, 14.9%, and 24.3%, respectively (<i>P</i> = 0.036). After adjusting for all confounding variables, the OR (95%CI) of gravidity 2, 3, 4, and ≥ 5 for anemia were 0.89 (0.60-1.33), 0.81 (0.52-1.26), 0.75 (0.45-1.24), and 0.52 (0.30-0.88), respectively. The findings of the present study showed that although the mean gravidity in the control group was significantly higher than that in the case group, women with gravidity ≥ 5 had a lower risk of developing anemia than those with only one pregnancy.</p>","PeriodicalId":49188,"journal":{"name":"Indian Journal of Hematology and Blood Transfusion","volume":"41 3","pages":"565-570"},"PeriodicalIF":0.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12267722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144676214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}