Modulation of Redox Metabolism, CD147, and CD47 Expression with the Maturation of Hematopoietic Stem Cells in Bone Marrow.

Haematopoiesis is a continuous process that involves the commitment of pluripotent hematopoietic stem cells (HSCs) into multipotent progenitors (MPPs) and differentiation into functional blood cells. The current study aimed to investigate the prevalence of various HSC progenitors in the bone marrow (BM) as well as alterations in the production of reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and surface expression level of basigin (CD147) and integrin-associated protein (CD47) receptors on various HSC lineages. Different HSC progenitors (GMP, CMP, MEP, MPP, ST-HSC, and LT-HSC) cells were recognised based on the surface expression of CD117/Sca-1/CD16/32/CD34 and CD117/Sca-1/CD135/CD34 receptors by flow cytometric analysis. Our results suggest that among Sca-1^- cells, the proportion of MPP cells was significantly higher than STHSC and LTHSC. Among Sca1⁺ cells, the GMP cells were more prevalent, approximately three times more than CMP, while MEP cells were least in proportion. The HSC maturation leads to a significant change in metabolism and surface receptor expression. The LTHSC and STHSC have similar ROS levels, but it was 7 times greater on MPP. The ROS level also increased from CMP to GMP transition (34% increase), but it was reduced significantly on MEP. MMP was significantly higher on MPP and GMP as compared to LTHSC and STHSC, and CMP and MEP, respectively. The surface expression of CD147 was increased significantly from LTHSC/STHSC to MPP and CMP to GMP transition. CD47 receptor expression was also increased during the maturation of HSC. It was almost similar on LTHSC and STHSC but increased on MPP. Similarly, CMP and MEP cells have equivalent CD47 expression but are upregulated on GMP. Overall, HSC maturation involves a series of metabolic changes, which regulate HSC differentiation into progenitor cells in the BM.