The Release of Platelet DAMPs is Regulated by NINJ1-Mediated Plasma Membrane Rupture.

Abstract

Damage-associated molecular patterns (DAMPs) can cause platelet activation and coagulation system dysfunction. It is often released by broken cell membranes after cell death. The oligomerization of ninjurin-1 (NINJ1) is closely associated with platelet plasma membrane rupture, but whether it actually prevents the release of platelet DAMPs is unclear. The aim of this study was to evaluate whether NINJ1 deficiency can maintain the integrity of dead platelet cell membranes. The release of extracellular DAMPs was detected by ELISA, and the release of platelet particles was detected by flow cytometry. In order to further evaluate the potential effect of inhibiting platelet DAMPs release on the regulation of inflammatory diseases, we extracted platelet supernatant after agonist-induced death and co-culture with endothelial cells. It was confirmed that the inflammatory secretion of endothelial cells induced by NINJ1^-/- platelet supernatant was significantly reduced. These data suggest that NINJ1 deletion restriction inhibits platelet DAMPs release, suggesting a potential target for the treatment of related diseases.