Microbial Biotechnology最新文献

{"title":"CD-g-CS nanoparticles for enhanced antibiotic treatment of Staphylococcus xylosus infection","authors":"Si-Di Zheng,&nbsp;Zhi-Yun Zhang,&nbsp;Jin-Xin Ma,&nbsp;Qian-Wei Qu,&nbsp;Bello-Onaghise God'spowe,&nbsp;Yue Qin,&nbsp;Xue-Ying Chen,&nbsp;LU Li,&nbsp;Dong-Fang Zhou,&nbsp;Wen-Ya Ding,&nbsp;Yan-Hua Li","doi":"10.1111/1751-7915.13870","DOIUrl":"https://doi.org/10.1111/1751-7915.13870","url":null,"abstract":"<p><i>Staphylococcus xylosus</i> (<i>S. xylosus</i>)-induced cow mastitis is an extremely serious clinical problem. However, antibiotic therapy does not successfully treat <i>S. xylosus</i> infection because these bacteria possess a strong biofilm formation ability, which significantly reduces the efficacy of antibiotic treatments. In this study, we developed ceftiofur-loaded chitosan grafted with β-cyclodextrins (CD-g-CS) nanoparticles (CT-NPs) using host–guest interaction. These positively charged nanoparticles improved bacterial internalization, thereby significantly improving the effectiveness of antibacterial treatments for planktonic <i>S. xylosus</i>. Moreover, CT-NPs effectively inhibited biofilm formation and eradicated mature biofilms. After mammary injection in a murine model of <i>S. xylosus</i>-induced mastitis, CT-NPs significantly reduced bacterial burden and alleviated inflammation, thereby achieving optimized therapeutic efficiency for <i>S. xylosus</i> infection. In conclusion, this treatment strategy could improve the efficiency of antibiotic therapeutics and shows great potential in the treatment of <i>S. xylosus</i> infections.</p>","PeriodicalId":49145,"journal":{"name":"Microbial Biotechnology","volume":"15 2","pages":"535-547"},"PeriodicalIF":5.7,"publicationDate":"2021-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/1751-7915.13870","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5757953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"emPAI-assisted strategy enhances screening and assessment of Mycobacterium tuberculosis infection serological markers","authors":"Guorong Ma,&nbsp;Pei Wang,&nbsp;Yanhui Yang,&nbsp;Wei Wang,&nbsp;Jinhua Ma,&nbsp;Lin Zhou,&nbsp;Junlin Ouyang,&nbsp;Rongxiu Li,&nbsp;Shulin Zhang","doi":"10.1111/1751-7915.13829","DOIUrl":"https://doi.org/10.1111/1751-7915.13829","url":null,"abstract":"<p>Discovering new serological markers of <i>Mycobacterium tuberculosis</i> (MTB) infection and establishing a rapid and efficient detection technology is of great significance for the prevention and control of tuberculosis. In this study, we established an exponentially modified protein abundance index (emPAI) value-assisted strategy to investigate and improve the screening efficiency of serological biomarkers of tuberculosis. First, we used LC-MS/MS to analyse MTB culture filtrate proteins (MTB-CFPs), and 632 MTB proteins were identified. Then, the characteristic values of MTB-CFPs – including emPAI value, molecular weight (Mw), isoelectric point (pI), grand average of hydropathy (GRAVY), transmembrane domain (TMD) and functional groups were calculated. Next, we successfully prepared 10 MTB proteins with emPAI value &gt; 1.0 and recombinantly expressed these proteins in <i>Escherichia coli</i>. At the same time, 3 MTB proteins with emPAI between 0.1 and 0.5 were randomly selected as the control groups, and the immunogenicity of the recombinant MTB proteins was detected using ELISA. The sensitivity and receiver operating characteristic (ROC) curves were calculated for each recombinant MTB protein. The results showed that the areas under the curve (AUC) value of Rv2031c, Rv0577, Rv0831c, Rv0934 and Rv3248c were all higher than those of Rv3875 (AUC, 0.6643). Further analysis of the relationship between emPAI value and antibody sensitivity, AUC value and antibody affinity in mice immunized with recombinant MTB protein showed that emPAI values were positively correlated with them, and R-squared value ranged from 0.64 to 0.79. The only exception was ESAT-6 (encoded by the Rv3875 gene), which AUC value was relatively low owing to its strong immunosuppressive properties. This study provides a rationale for the serological marker screening of emPAI-assisted tuberculosis clinical test. The results also provide new technical support for the screening of candidate serological markers of infectious diseases in the future.</p>","PeriodicalId":49145,"journal":{"name":"Microbial Biotechnology","volume":"14 4","pages":"1827-1838"},"PeriodicalIF":5.7,"publicationDate":"2021-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/1751-7915.13829","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5836224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Importance of the 5′ regulatory region to bacterial synthetic biology applications","authors":"Lisa Tietze,&nbsp;Rahmi Lale","doi":"10.1111/1751-7915.13868","DOIUrl":"https://doi.org/10.1111/1751-7915.13868","url":null,"abstract":"<p>The field of synthetic biology is evolving at a fast pace. It is advancing beyond single-gene alterations in single hosts to the logical design of complex circuits and the development of integrated synthetic genomes. Recent breakthroughs in deep learning, which is increasingly used in <i>de novo</i> assembly of DNA components with predictable effects, are also aiding the discipline. Despite advances in computing, the field is still reliant on the availability of pre-characterized DNA parts, whether natural or synthetic, to regulate gene expression in bacteria and make valuable compounds. In this review, we discuss the different bacterial synthetic biology methodologies employed in the creation of 5′ regulatory regions – promoters, untranslated regions and 5′-end of coding sequences. We summarize methodologies and discuss their significance for each of the functional DNA components, and highlight the key advances made in bacterial engineering by concentrating on their flaws and strengths. We end the review by outlining the issues that the discipline may face in the near future.</p>","PeriodicalId":49145,"journal":{"name":"Microbial Biotechnology","volume":"14 6","pages":"2291-2315"},"PeriodicalIF":5.7,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/1751-7915.13868","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5713976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trichoderma harzianum cerato-platanin enhances hydrolysis of lignocellulosic materials","authors":"Anna Pennacchio,&nbsp;Rossana Pitocchi,&nbsp;Giovanna Cristina Varese,&nbsp;Paola Giardina,&nbsp;Alessandra Piscitelli","doi":"10.1111/1751-7915.13836","DOIUrl":"https://doi.org/10.1111/1751-7915.13836","url":null,"abstract":"<p>Considering its worldwide abundance, cellulose can be a suitable candidate to replace the fossil oil-based materials, even if its potential is still untapped, due to some scientific and technical gaps. This work offers new possibilities demonstrating for the first time the ability of a cerato-platanin, a small fungal protein, to valorize lignocellulosic Agri-food Wastes. Indeed, cerato-platanins can loosen cellulose rendering it more accessible to hydrolytic attack. The cerato-platanin <i>Th</i>CP from a marine strain of <i>Trichoderma harzianum</i>, characterized as an efficient biosurfactant protein, has proven able to efficiently pre-treat apple pomace, obtaining a sugar conversion yield of 65%. Moreover, when used in combination with a laccase enzyme, a notable increase in the sugar conversion yield was measured. Similar results were also obtained when other wastes, coffee silverskin and potato peel, were pre-treated. With respect to the widespread laccase pre-treatments, this new pre-treatment approach minimizes process time, increasing energy efficiency.</p>","PeriodicalId":49145,"journal":{"name":"Microbial Biotechnology","volume":"14 4","pages":"1699-1706"},"PeriodicalIF":5.7,"publicationDate":"2021-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/1751-7915.13836","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5778564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Benchmarking recombinant Pichia pastoris for 3-hydroxypropionic acid production from glycerol","authors":"Albert Fina,&nbsp;Gabriela Coelho Brêda,&nbsp;Míriam Pérez-Trujillo,&nbsp;Denise Maria Guimar?es Freire,&nbsp;Rodrigo Volcan Almeida,&nbsp;Joan Albiol,&nbsp;Pau Ferrer","doi":"10.1111/1751-7915.13833","DOIUrl":"https://doi.org/10.1111/1751-7915.13833","url":null,"abstract":"<p>The use of the methylotrophic yeast <i>Pichia pastoris</i> (<i>Komagataella phaffi</i>) to produce heterologous proteins has been largely reported. However, investigations addressing the potential of this yeast to produce bulk chemicals are still scarce. In this study, we have studied the use of <i>P. pastoris</i> as a cell factory to produce the commodity chemical 3-hydroxypropionic acid (3-HP) from glycerol. 3-HP is a chemical platform which can be converted into acrylic acid and to other alternatives to petroleum-based products. To this end, the <i>mcr</i> gene from <i>Chloroflexus aurantiacus</i> was introduced into <i>P. pastoris</i>. This single modification allowed the production of 3-HP from glycerol through the malonyl-CoA pathway. Further enzyme and metabolic engineering modifications aimed at increasing cofactor and metabolic precursors availability allowed a 14-fold increase in the production of 3-HP compared to the initial strain. The best strain (PpHP6) was tested in a fed-batch culture, achieving a final concentration of 3-HP of 24.75 g l⁻¹, a product yield of 0.13 g g⁻¹ and a volumetric productivity of 0.54 g l⁻¹ h⁻¹, which, to our knowledge, is the highest volumetric productivity reported in yeast. These results benchmark <i>P. pastoris</i> as a promising platform to produce bulk chemicals for the revalorization of crude glycerol and, in particular, to produce 3-HP.</p>","PeriodicalId":49145,"journal":{"name":"Microbial Biotechnology","volume":"14 4","pages":"1671-1682"},"PeriodicalIF":5.7,"publicationDate":"2021-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/1751-7915.13833","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5683530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioproduction of L-piperazic acid in gram scale using Aureobasidium melanogenum","authors":"Cuncui Kong,&nbsp;Zhuangzhuang Wang,&nbsp;Guanglei Liu,&nbsp;Zhenming Chi,&nbsp;Rodrigo Ledesma-Amaro,&nbsp;Zhe Chi","doi":"10.1111/1751-7915.13838","DOIUrl":"https://doi.org/10.1111/1751-7915.13838","url":null,"abstract":"Currently, piperazic acid is chemically synthesized using ecologically unfriendly processes. Microbial synthesis from glucose is an attractive alternative to chemical synthesis. In this study, we report the production of L‐piperazic acid via microbial fermentation with the first engineered fungal strain of Aureobasidium melanogenum; this strain was constructed by chassis development, genetic element reconstitution and optimization, synthetic rewiring and constitutive genetic circuit reconstitution, to build a robust L‐piperazic acid synthetic cascade. These genetic modifications enable A. melanogenum to directly convert glucose to L‐piperazic acid without relying on the use of either chemically synthesized precursors or harsh conditions. This bio‐based process overcomes the shortcomings of the conventional synthesis routes. The ultimately engineered strain is a very high‐efficient cell factory that can excrete 1.12 ± 0.05 g l‐1 of L‐piperazic acid after a 120‐h 10.0‐l fed‐batch fermentation; this is the highest titre of L‐piperazic acid reported using a microbial cell factory.","PeriodicalId":49145,"journal":{"name":"Microbial Biotechnology","volume":"14 4","pages":"1722-1729"},"PeriodicalIF":5.7,"publicationDate":"2021-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/1751-7915.13838","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6103666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rapid MALDI-TOF mass spectrometry-based method for colistin susceptibility testing in Escherichia coli","authors":"Jiaping Li,&nbsp;Yonglu Huang,&nbsp;Yanyan Hu,&nbsp;Qiaoling Sun,&nbsp;Jiachang Cai,&nbsp;Hongwei Zhou,&nbsp;Danxia Gu,&nbsp;Gongxiang Chen,&nbsp;Yang Wang,&nbsp;Rong Zhang","doi":"10.1111/1751-7915.13826","DOIUrl":"https://doi.org/10.1111/1751-7915.13826","url":null,"abstract":"<p>Colistin is recognized as a last-resort treatment option against multi-drug resistant bacteria including carbapenem-resistant <i>Enterobacteriaceae</i> (CRE). However, the plasmid-mediated colistin-resistance gene <i>mcr-1</i> has been reported globally resulting in an increase of colistin-resistant bacteria. A quick and accurate method for determining the pathogen resistance of colistin is therefore crucial in the clinic. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a potential tool forto be applied for antimicrobial susceptibility testing. We compared the growth of <i>Escherichia coli</i> strains in the presence or absence of colistin. Automated analyses of the spectra were performed with a prototype software tool written with package R. Three <i>mcr-1</i>-positive and six <i>mcr-1-</i>negative <i>E. coli</i> were used for establishing the model to obtain the optimal incubation time, the breakpoint concentration of colistin and cut-off of the relative growth (RG) value. The distinction between susceptible and resistant strains was already noticeable after 2 h of incubation. The best separation between the susceptible and resistant strains was achieved at a concentration of 4 µg ml^-1 and a relative growth cut-off value of 0.6. Application of the model for the analysis of 128 <i>E</i>. <i>coli</i> isolates, a sensitivity of 97.4% and a specificity of 88.2% were achieved compared with colistin MIC results. The rapid MALDI-TOF MS-based method approach is simple to set-up, uses a short incubation time, and had excellent outcomes with respect to sensitivity and specificity for colistin sensitivity testing in <i>Escherichia coli</i>.</p>","PeriodicalId":49145,"journal":{"name":"Microbial Biotechnology","volume":"15 2","pages":"528-534"},"PeriodicalIF":5.7,"publicationDate":"2021-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/1751-7915.13826","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5735208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic engineering biofilms in situ using ultrasound-mediated DNA delivery","authors":"Chun Kiat Ng,&nbsp;Samuel L. Putra,&nbsp;Joseph Kennerley,&nbsp;Robert Habgood,&nbsp;Ronald A. Roy,&nbsp;Jason L. Raymond,&nbsp;Ian P. Thompson,&nbsp;Wei E. Huang","doi":"10.1111/1751-7915.13823","DOIUrl":"https://doi.org/10.1111/1751-7915.13823","url":null,"abstract":"<p>The ability to directly modify native and established biofilms has enormous potential in understanding microbial ecology and application of biofilm in 'real-world' systems. However, efficient genetic transformation of established biofilms at any scale remains challenging. In this study, we applied an ultrasound-mediated DNA delivery (UDD) technique to introduce plasmid to established non-competent biofilms <i>in situ</i>. Two different plasmids containing genes coding for superfolder green fluorescent protein (sfGFP) and the flavin synthesis pathway were introduced into established bacterial biofilms in microfluidic flow (transformation efficiency of 3.9 ± 0.3 × 10^-7 cells in biofilm) and microbial fuel cells (MFCs), respectively, both employing UDD. Gene expression and functional effects of genetically modified bacterial biofilms were observed, where some cells in UDD-treated <i>Pseudomonas putida</i> UWC1 biofilms expressed sfGFP in flow cells and UDD-treated <i>Shewanella oneidensis</i> MR-1 biofilms generated significantly (<i>P</i> &lt; 0.05) greater (61%) bioelectricity production (21.9 ± 1.2 µA cm⁻²) in MFC than a wild-type control group (~ 13.6 ± 1.6 µA cm⁻²). The effects of UDD were amplified in subsequent growth under selection pressure due to antibiotic resistance and metabolism enhancement. UDD-induced gene transfer on biofilms grown in both microbial flow cells and MFC systems was successfully demonstrated, with working volumes of 0.16 cm³ and 300 cm³, respectively, demonstrating a significant scale-up in operating volume. This is the first study to report on a potentially scalable direct genetic engineering method for established non-competent biofilms, which can be exploited in enhancing their capability towards environmental, industrial and medical applications.</p>","PeriodicalId":49145,"journal":{"name":"Microbial Biotechnology","volume":"14 4","pages":"1580-1593"},"PeriodicalIF":5.7,"publicationDate":"2021-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/1751-7915.13823","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5661594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Repurposing the FDA-approved anticancer agent ponatinib as a fluconazole potentiator by suppression of multidrug efflux and Pma1 expression in a broad spectrum of yeast species","authors":"Lin Liu,&nbsp;Tong Jiang,&nbsp;Jia Zhou,&nbsp;Yikun Mei,&nbsp;Jinyang Li,&nbsp;Jingcong Tan,&nbsp;Luqi Wei,&nbsp;Jingquan Li,&nbsp;Yibing Peng,&nbsp;Changbin Chen,&nbsp;Ning-Ning Liu,&nbsp;Hui Wang","doi":"10.1111/1751-7915.13814","DOIUrl":"https://doi.org/10.1111/1751-7915.13814","url":null,"abstract":"<p>Fungal infections have emerged as a major global threat to human health because of the increasing incidence and mortality rates every year. The emergence of drug resistance and limited arsenal of antifungal agents further aggravates the current situation resulting in a growing challenge in medical mycology. Here, we identified that ponatinib, an FDA-approved antitumour drug, significantly enhanced the activity of the azole fluconazole, the most widely used antifungal drug. Further detailed investigation of ponatinib revealed that its combination with fluconazole displayed broad-spectrum synergistic interactions against a variety of human fungal pathogens such as <i>Candida albicans</i>, <i>Saccharomyces cerevisiae</i> and <i>Cryptococcus neoformans</i>. Mechanistic insights into the mode of action unravelled that ponatinib reduced the efflux of fluconazole via Pdr5 and suppressed the expression of the proton pump, Pma1. Taken together, our study identifies ponatinib as a novel antifungal that enhances drug activity of fluconazole against diverse fungal pathogens.</p>","PeriodicalId":49145,"journal":{"name":"Microbial Biotechnology","volume":"15 2","pages":"482-498"},"PeriodicalIF":5.7,"publicationDate":"2021-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/1751-7915.13814","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6119630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flexible integrated sensing platform for monitoring wound temperature and predicting infection","authors":"Yuheng Zhang,&nbsp;Bin Lin,&nbsp;Rong Huang,&nbsp;Zhixiao Lin,&nbsp;Yongqian Li,&nbsp;Jinqing Li,&nbsp;Xueyong Li","doi":"10.1111/1751-7915.13821","DOIUrl":"https://doi.org/10.1111/1751-7915.13821","url":null,"abstract":"<p>Wound infection is a challenging clinical problem that imposes substantial economic and psychological burdens on patients. However, the wound covered by a dressing is in an ‘unknown’ state. Recently, researchers have focused on understanding the condition of the wound without removing the dressing. Here, we presented a flexible integrated sensing platform (FISP) that can monitor multiple indicators, including local temperature. The platform consists of a flexible sensor chip (FSC), a controlled printed circuit board (CPCB) and a customized application installed on a smartphone that can receive and display data from the sensor chip through Bluetooth Low Energy 4.0 (BLE4.0) and upload real-time wound information. This device exhibits satisfactory measurement accuracy, stability, durability, skin compliance and biocompatibility. It was applied to infected wounds on the back of rabbits to reveal the temperature changes characteristic of wounds infected with different bacteria, and this information was compared with the changes in the core body temperature of animals. We found differences in the temperature among wounds infected with different pathogens and the temperature of the wound infection occurred earlier than the change in anal temperature. The combined application of the FISP and dressings might help identify the ‘unknown’ state of wounds in the clinic.</p>","PeriodicalId":49145,"journal":{"name":"Microbial Biotechnology","volume":"14 4","pages":"1566-1579"},"PeriodicalIF":5.7,"publicationDate":"2021-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/1751-7915.13821","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"6092232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}