CD-g-CS nanoparticles for enhanced antibiotic treatment of Staphylococcus xylosus infection

IF 4.8 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Microbial Biotechnology Pub Date : 2021-06-28 DOI:10.1111/1751-7915.13870

Si-Di Zheng, Zhi-Yun Zhang, Jin-Xin Ma, Qian-Wei Qu, Bello-Onaghise God'spowe, Yue Qin, Xue-Ying Chen, LU Li, Dong-Fang Zhou, Wen-Ya Ding, Yan-Hua Li

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引用次数: 2

Abstract

Staphylococcus xylosus (S. xylosus)-induced cow mastitis is an extremely serious clinical problem. However, antibiotic therapy does not successfully treat S. xylosus infection because these bacteria possess a strong biofilm formation ability, which significantly reduces the efficacy of antibiotic treatments. In this study, we developed ceftiofur-loaded chitosan grafted with β-cyclodextrins (CD-g-CS) nanoparticles (CT-NPs) using host–guest interaction. These positively charged nanoparticles improved bacterial internalization, thereby significantly improving the effectiveness of antibacterial treatments for planktonic S. xylosus. Moreover, CT-NPs effectively inhibited biofilm formation and eradicated mature biofilms. After mammary injection in a murine model of S. xylosus-induced mastitis, CT-NPs significantly reduced bacterial burden and alleviated inflammation, thereby achieving optimized therapeutic efficiency for S. xylosus infection. In conclusion, this treatment strategy could improve the efficiency of antibiotic therapeutics and shows great potential in the treatment of S. xylosus infections.

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CD-g-CS纳米颗粒增强抗生素治疗木糖葡萄球菌感染

木糖葡萄球菌(S. xylosus)引起的奶牛乳腺炎是一种极为严重的临床问题。然而，抗生素治疗并不能成功治疗木葡萄球菌感染，因为这些细菌具有很强的生物膜形成能力，这大大降低了抗生素治疗的效果。在这项研究中，我们利用主客体相互作用开发了负载头孢替呋喃的壳聚糖接枝β-环糊精纳米颗粒(CD-g-CS)。这些带正电的纳米颗粒改善了细菌的内化，从而显著提高了浮游木藻的抗菌治疗效果。此外，CT-NPs有效地抑制了生物膜的形成并根除了成熟的生物膜。CT-NPs在木糖葡萄球菌诱导的小鼠乳腺炎模型中乳腺注射后，可显著降低细菌负担，减轻炎症，从而达到最佳的治疗效果。综上所述，该治疗策略可以提高抗生素治疗的效率，在木霉菌感染的治疗中具有很大的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbial Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY

CiteScore

9.80

自引率

3.50%

发文量

162

审稿时长

6-12 weeks

期刊介绍： Microbial Biotechnology publishes papers of original research reporting significant advances in any aspect of microbial applications, including, but not limited to biotechnologies related to: Green chemistry; Primary metabolites; Food, beverages and supplements; Secondary metabolites and natural products; Pharmaceuticals; Diagnostics; Agriculture; Bioenergy; Biomining, including oil recovery and processing; Bioremediation; Biopolymers, biomaterials; Bionanotechnology; Biosurfactants and bioemulsifiers; Compatible solutes and bioprotectants; Biosensors, monitoring systems, quantitative microbial risk assessment; Technology development; Protein engineering; Functional genomics; Metabolic engineering; Metabolic design; Systems analysis, modelling; Process engineering; Biologically-based analytical methods; Microbially-based strategies in public health; Microbially-based strategies to influence global processes