Technology and Health Care最新文献

{"title":"LINC01004/hsa-mir-125b-2-3p axis restrains ferroptosis in hepatocellular carcinoma by targeting HSPA4 via ceRNA mechanism.","authors":"Han Gao, Li Fan, Xue Gai, Rong Fu, Guohua Li, Kexin Jing, Jingwei Xu, Shengjian Sun","doi":"10.1177/09287329241291430","DOIUrl":"10.1177/09287329241291430","url":null,"abstract":"<p><p>BackgroundHepatocellular carcinoma (HCC) is a primary cancer, accounting for 90% of primary liver cancer, mainly occurring in patients with cirrhosis and chronic liver disease.ObjectiveTo investigate the latent mechanisms of hepatocellular carcinoma (HCC) and find therapeutic targets.MethodsDifferentially expressed and overall survival related genes of HCC, and cell death related genes were intersected to obtain latent target genes. These genes were analyzed using ROC curve for diagnosing HCC. RT-qPCR and Western blot were performed to detect the expression level of genes. Wound healing tests were performed with or without si-HSPA4. Potential ceRNA axis was forecasted using TargetScan and miRanda and the dual luciferase reporter gene assay was used to verify the results. Finally, the images of H&E dye liquor-stained HCC tissue section, the CT images for patients in different tumor stage.ResultsLINC01004/hsa-miR-125b-2-3p/HSPA4 axis was forecasted and then was verified using dual-luciferase reporter assay. HSPA4 knockdown caused significant reduction of cell proliferation and ferroptosis. Si-HSPA4 related ferroptosis was generated through impairing iron transport via targeting restrain GPX4. For human subjects, the RT-qPCR analysis revealed the that the larger the tumor diameter, the higher the LINC01004, HSPA4, and GPX4 expression, and the lower the hsa-miR-125b-2-3p expression.ConclusionLINC01004/hsa-miR-125b-2-3p/HSPA4 regulatory axis involved in the ferroptosis of the progression of HCC via GPX4 dependent method, providing new therapeutic targets for HCC patients.</p>","PeriodicalId":48978,"journal":{"name":"Technology and Health Care","volume":"33 2","pages":"959-973"},"PeriodicalIF":1.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive value of mNUTRIC score for chronic critical illness in patients of sepsis complicated with ARDS.","authors":"Mingxia Ji, Linlin Zhu, Mengyan Chen, Yi Wang, Weiyong Qiu, Ning Zhang","doi":"10.1177/09287329241296430","DOIUrl":"10.1177/09287329241296430","url":null,"abstract":"<p><p>ObjectiveTo explore the predictive value of the modified NUTrition Risk in the Critically ill (mNUTRIC) for patients with sepsis and acute respiratory distress syndrome (ARDS) developing into chronic critical illness (CCI).BackgroundSepsis is an important risk factor for Acute respiratory distress syndrome (ARDS), and sepsis induced ARDS accounts for 32% of patients. Patients with this subtype of ARDS have a worse prognosis than those with sepsis alone or ARDS, usually facing higher mortality rates and lower success rates of extubation, and have received more attention in clinical practice.MethodsA retrospective observational study was conducted on 118 patients admitted to the Intensive Care Unit (ICU) of Yiwu Central Hospital from January 2020 to May 2022. Patients were categorized into two groups: CCI and rapid recovery (RAP), based on the occurrence of CCI. Age, gender, white blood cell count, C-reactive protein, albumin, serum creatinine, sequential organ failure assessment (SOFA), acute physiology and chronic health evaluation II (APACHE II), mNUTRIC score, ICU length of stay, mechanical ventilation duration, and continuous renal replacement therapy (CRRT) were compared between the two groups. The association between mNUTRIC and CCI in patients with sepsis complicated by ARDS was further examined using three models and multivariate logistic regression.The receiver operating characteristic (ROC) curve was utilized to analyze the predictive values of mNUTRIC score, SOFA score, and APACHE II score for predicting the occurrence of CCI in patients with sepsis complicated by ARDS.ResultsThe age, mNUTRIC score, SOFA score, APACHE II score, ICU length of stay, mechanical ventilation duration, and CRRT ratio were significantly higher in the CCI group compared to the RAP group (all P < 0.05). Conversely, there were no statistically significant differences in white blood cell count, C-reactive protein, and albumin levels between the two groups (all P > 0.05). Multivariate logistic regression analysis identified the mNUTRIC score as an independent risk factor for CCI in patients with sepsis complicated by ARDS. The constructed models showed that the mNUTRIC score remained significant, while the SOFA score and APACHE II score did not.The area under the ROC curve analysis demonstrated that the mNUTRIC score had the highest AUC of 0.880, 95% Confidence Interval (CI): 0.808-0.933. The AUC for the SOFA score was 0.864, and for the APACHE II score was 0.825, all of which were statistically significant.ConclusionsThe mNUTRIC score is valuable in predicting the progression of sepsis combined with ARDS to CCI, and may even be superior to the SOFA score and APACHE II score.</p>","PeriodicalId":48978,"journal":{"name":"Technology and Health Care","volume":" ","pages":"831-837"},"PeriodicalIF":1.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143460310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The application of flipped classroom teaching model combining scientific research expansion in the teaching of thoracic surgery.","authors":"Zhu Liang, Chunyuan Chen, Zhanqiang Xie, Zhigang Wang, Xiaobi Cai, Lin Chen, Dinghua Xu","doi":"10.1177/09287329241290319","DOIUrl":"10.1177/09287329241290319","url":null,"abstract":"<p><p>BackgroundThe Department of Thoracic Surgery of the First Clinical Medical College of Guangdong Medical University took the lead in introducing the flipped classroom teaching mode combining scientific research expansion into the undergraduate teaching of clinical medicine in 2018. This teaching mode has achieved good teaching effects.ObjectiveThrough the flipped classroom based on role exchange, it is observed whether students can achieve a comprehensive understanding of lung cancer, whether they have basic scientific research quality, innovative application ability and stimulate students' enthusiasm for learning.MethodsDuring the research process, teachers prepare their lessons meticulously. Students interpret the study materials line by line as required, identify difficulties, and engage in discussions to resolve them. If unable to resolve, they can ask questions through various channels such as QQ, WeChat, or email. This allows students to choose their study time independently, achieving personalized learning. Teachers adjust in real-time and effectively formulate teaching plans, objectives, and progress.ResultsFor this teaching method reform, most students believed that this teaching improved their teamwork skills, PPT creation skills, and active problem-solving abilities, improved their understanding of scientific research, stimulated their interest in scientific research, deepened their understanding and understanding of lung cancer diagnosis and treatment, and enhanced their enthusiasm for learning.ConclusionThis not only stimulated students' enthusiasm for learning thoracic surgery but also improved their scientific research and clinical thinking abilities, resulting in good teaching outcomes.</p>","PeriodicalId":48978,"journal":{"name":"Technology and Health Care","volume":" ","pages":"755-761"},"PeriodicalIF":1.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143460343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutritional counseling's impact on muscle mass and quality of life in stage 4 chronic kidney disease malnourished patients.","authors":"Weiyan Sun, Lingxi Liu","doi":"10.1177/09287329241291370","DOIUrl":"10.1177/09287329241291370","url":null,"abstract":"<p><p>BackgroundChronic Kidney Disease (CKD) is a significant public health issue, affecting 37 million people in the United States. CKD often leads to malnutrition, particularly in advanced stages, contributing to muscle wasting, reduced quality of life, and increased healthcare costs.ObjectiveThis retrospective study aimed to investigate the impact of early nutritional intervention and counseling on muscle mass and quality of life in malnourished stage 4 chronic kidney disease (CKD) patients.MethodsClinical data from stage 4 CKD patients with concomitant malnutrition admitted to a single center from January 2023 to December 2023 were retrospectively analyzed. Patients were divided into a standard intervention group and an early nutritional intervention and counseling group. Baseline characteristics, muscle mass parameters, quality of life assessments, nutritional parameters, and adverse events were compared between the two groups.ResultsThe baseline characteristics of the study participants were well-balanced between the two groups. The early nutrition intervention group demonstrated significant improvements in muscle mass parameters, including grip strength, skeletal muscle mass, muscle strength, calf circumference, and handgrip measurement, compared to the standard intervention group. Additionally, the early nutrition intervention group showed significant enhancements in several aspects of quality of life, including physical functioning, mental health, energy/fatigue, and general health scores. Improvements in nutritional parameters, such as protein intake, caloric intake, vitamin D levels, iron levels, and zinc levels, were also observed in the early nutrition intervention group. Notably, no significant differences in adverse events were found between the two groups.ConclusionThe study highlights the significant benefits of early nutritional intervention and counseling for malnourished stage 4 CKD patients. Early nutritional support positively impacts muscle mass, quality of life, and nutritional parameters. These findings emphasize the importance of nutrition in CKD management and the need for comprehensive nutritional support as a key component of care. This research paves the way for further studies and clinical initiatives to enhance nutritional care and improve the standard of care for CKD patients.</p>","PeriodicalId":48978,"journal":{"name":"Technology and Health Care","volume":"33 2","pages":"951-958"},"PeriodicalIF":1.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of duloxetine for the treatment of vestibular migraine.","authors":"Tengteng Zhang, Shuping Zhang, Jinghui Sun, He Zhang, Luting Lv, Jie Li, Jiandong Wang, Zhidan Yu, Fei Che, Shuqin Wu","doi":"10.1177/09287329241291686","DOIUrl":"10.1177/09287329241291686","url":null,"abstract":"<p><p>BackgroundMigraine is characterized by unilateral head pain with episodic attacks interspersed with symptom-free periods. This recurring and disabling neurovascular condition affects 18% of women and 6% of men, with a global prevalence of 2% for chronic migraine.ObjectiveThis study aims to estimate the effectiveness of duloxetine in treating vestibular migraine.MethodsThe study included a total of 72 patients who were admitted to the Neurology department between January 2020 and January 2022. Based on the presence or absence of duloxetine in their medical records, the patients were categorized into two groups: a duloxetine group and a control group. Parameters used to assess treatment effects on vestibular symptoms included DHI, VSS, SAS, SRSS, SF-36, and BPI. Follow-up and statistical analysis of all data were performed.ResultsDuloxetine treatment significantly reduced VSS, the DHI total score, and the number of vertiginous episodes in VM patients, while the other medicine treatment also decreased the incidence of vertiginous attacks. However, the reduction in duloxetine group presented a better treatment effect and a lower occurrence of adverse reactions contrasted to the control group.<b>Conclusions:</b> This study indicates that duloxetine treatment offers significant improvements in comparison to other medicine treatment. These results align with previous research on migraine prevention and contribute to the mounting evidence that supports duloxetine was used to treat vestibular migraine.</p>","PeriodicalId":48978,"journal":{"name":"Technology and Health Care","volume":"33 2","pages":"838-847"},"PeriodicalIF":1.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary study: Low to moderate PEEP effects on ICP in acute neurological injury patients under mechanical ventilation.","authors":"Nan Xiu","doi":"10.1177/09287329241291322","DOIUrl":"10.1177/09287329241291322","url":null,"abstract":"<p><p>BackgroundAcute respiratory distress syndrome (ARDS) significantly impacts mortality and prognosis in acute neurological injury patients. Positive end-expiratory pressure (PEEP) is essential in mechanical ventilation to enhance oxygenation, yet its application may elevate intracranial pressure (ICP). Studies show conflicting findings regarding PEEP's effect on ICP, particularly at low to moderate levels (5-10cmH2O), warranting further investigation in acute neurological injury cases.ObjectiveTo assess the impact of low to moderate PEEP on ICP in patients with acute neurological injury who require mechanical ventilation.MethodsA retrospective analysis of 62 patients with acute neurological injury requiring mechanical ventilation at our hospital between January 2021 and September 2023 was conducted. Patients were divided into high PEEP (> 10cmH2O) and low to moderate PEEP (5-10cmH2O) groups. Parameters including PaO2, PaCO2, PaO2/FiO2, driving pressure, compliance (Cst), airway resistance, such as heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP), central venous pressure (CVP), cardiac index (CI), ICP, and cerebral perfusion pressure (CPP) were recorded pre- and post-ventilation initiation, with correlation analysis performed between ICP and other parameters.ResultsPre-treatment comparisons between the high PEEP and low to moderate PEEP groups revealed no significant differences in PaO2, PaCO2, PaO2/FiO2, driving pressure, Cs, and airway resistance. However, post-treatment analysis showed significant disparities, with the high PEEP group exhibiting higher PaO2 and PaO2/FiO2 levels and lower PaCO2, driving pressure, and airway resistance levels compared to the low to moderate PEEP group. Additionally, hemodynamic parameters such as HR, SBP, DBP, CI, MAP, CVP, ICP, and CPP remained more stable in the low to moderate PEEP group post-treatment, with ICP demonstrating significant correlations with various physiological parameters.ConclusionHigh PEEP improves oxygenation and respiratory mechanics in acute neurological injury patients on mechanical ventilation but may affect hemodynamic parameters, ICP, and CPP. Conversely, low to moderate PEEP has minimal impact on these factors. Hence, personalized adjustment of PEEP levels is essential in clinical management.</p>","PeriodicalId":48978,"journal":{"name":"Technology and Health Care","volume":"33 2","pages":"981-988"},"PeriodicalIF":1.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemodynamic evaluation of a novel double lumen cannula for left ventricle assist device system.","authors":"Honglong Yu, Xuefeng Feng, Yao Xie, Qilian Xie, Hu Peng","doi":"10.1177/09287329241290947","DOIUrl":"10.1177/09287329241290947","url":null,"abstract":"<p><p>BackgroundThe left ventricular assist device (LVAD) has been proven to be an effective therapy for providing temporary circulatory support. However, the use of this device can cause myocardial injury due to multiple insertions of various catheters.ObjectiveTherefore, this study aimed to evaluate the hemodynamic performance of a newly developed double-lumen catheter (DLC) for LVAD.MethodsTwo different LVAD DLC prototypes (a semi-circular and a concentric catheter) were designed based on the structure of venous DLC. Computational fluid dynamics (CFD) simulations were performed using the finite element method. The CFD results were confirmed through the testing of the 31 Fr prototype. The aorta is a large vessel with shear rates up to >300 s^-1 and we used a reasonable approximation to model blood as a Newtonian fluid.ResultsAt a flow rate of 5 L/min, the semi-circular prototype achieved an infusion pressure of 74.68 mmHg, while the concentric prototype achieved an infusion pressure of 46.11 mmHg. The CFD results matched the experimental results with a mean percentage error of less than 7%. The peak wall shear stress in the semi-circular prototype (717.5 Pa) was higher than the hemolysis threshold (400 Pa), which could cause blood damage, and it also had a higher hemolysis index compared to concentric prototype. Moreover, both prototypes exhibited areas of blood stagnation and recirculation, suggesting a possible risk of thrombosis.ConclusionBoth prototypes of the LVAD DLC demonstrated similar blood flow rates. The semi-circular prototype showed superior infusion pressure compared to the concentric prototype, but had poorer hemolysis performance. However, the potential risk of thrombosis for both still exists. Therefore, further <i>in vivo</i> experiments are necessary to verify the safety and effectiveness of the LVAD DLC.</p>","PeriodicalId":48978,"journal":{"name":"Technology and Health Care","volume":" ","pages":"814-830"},"PeriodicalIF":1.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143460237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancement of post-market surveillance of medical devices leveraging artificial intelligence: Infusion pumps case study.","authors":"Nejra Merdović, Lemana Spahić, Madžida Hundur, Lejla Gurbeta Pokvić, Almir Badnjević","doi":"10.1177/09287329241291415","DOIUrl":"10.1177/09287329241291415","url":null,"abstract":"<p><p>BackgroundAnalysis of data from incident registries such as MAUDE has identified the need to improve surveillance and maintenance strategies for infusion pumps to enhance patient and healthcare staff safety.ObjectiveThe ultimate goal is to enhance infusion pump management strategies in healthcare facilities, thus transforming the current reactive approach to infusion pump management into a proactive and predictive one.<b>Method:</b> This study utilized real data collected from 2015 to 2021 through the inspection of infusion pumps in Bosnia and Herzegovina. Inspections were conducted by the national laboratory in accordance with the Legal Metrology Framework, accredited to ISO 17020 standard. Out of 988 samples, 790 were used for model training, while 198 samples were set aside for validation (20% of the dataset). Various machine learning algorithms for binary classification of samples (pass/fail status) were considered, including Logistic Regression, Decision Tree, Random Forest, Naive Bayes, and Support Vector Machine. These algorithms were chosen for their ability to handle large datasets and potential for high prediction accuracy.ResultsThrough detailed analysis of the achieved results, it was found that all applied machine learning methods yielded satisfactory results, with accuracy ranging from 0.98% to 1.0%, precision from 0.99% to 1%, sensitivity from 0.98% to 1.0%, and specificity from 0.87% to 1.0%. However, Decision Tree and Random Forest methods proved to be the best, both due to their maximum achieved values of accuracy, precision, sensitivity, and specificity, and due to result interpretability.ConclusionIt has been established that machine learning methods are capable of identifying potential issues before they become critical, thus playing a crucial role in predicting the performance of infusion pumps, potentially enhancing the safety, reliability, and efficiency of healthcare delivery. Further research is needed to explore the potential application of machine learning algorithms in various healthcare domains and to address practical issues related to the implementation of these algorithms in real clinical settings.</p>","PeriodicalId":48978,"journal":{"name":"Technology and Health Care","volume":"33 2","pages":"915-921"},"PeriodicalIF":1.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of wearable device technology in terms of health and safety in firefighters.","authors":"Güler Aksüt, Tamer Eren","doi":"10.1177/09287329241291385","DOIUrl":"10.1177/09287329241291385","url":null,"abstract":"<p><p>BackgroundFirefighting is one of the tasks that is physically difficult to perform and carries a high risk of injury and death. A better understanding of the underlying factors that influence the causes of fire scene injuries can improve firefighters' safety.ObjectiveFor this reason, the study aimed to determine the importance of Smart Personal Protective Equipment and wearable technology in protecting the health and safety of firefighters by using them instead of traditional equipment and systems. According to expert opinions and literature reviews, the dangers faced by firefighters have been determined to be thermal, physical, biological, environmental, and chemical.MethodsAnalytical Hierarchy Process (AHP) and Preference Ranking Organization Method for Enrichment Evaluations (PROMETHEE) methods were used in the study. The AHP method was preferred because it is a systematic decision-making method that includes both ranking and comparison techniques. The PROMETHEE method was preferred because it provides the opportunity to make effective decisions in a very short time by basing the decision-making process on a scientific basis. In addition to the graphical representation of the ranking of alternatives, it offers decision-makers the opportunity to make various statistical analyses.ResultsThe weights of the hazards were calculated using the AHP method. Physical hazards accounted for the highest weight. PROMETHEE was used in the ranking of wearable smart technological products to protect the health and safety of firefighters.<b>Conclusions:</b> Products are listed as Personal Protection System, PROeTEX PPE, Wearable IoT Device, Flame Resistant Shirt, Fall Detection Systems, Smart Wearable Underwear, and WASP. With the study, it was concluded that the risk of firefighters being trapped would play an essential role in the prevention of death and injury. Improvements in wearable technological products used in the fire department will yield better results and increase safety.</p>","PeriodicalId":48978,"journal":{"name":"Technology and Health Care","volume":" ","pages":"726-736"},"PeriodicalIF":1.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143460225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the genetic mysteries of sarcopenia: A bioinformatics approach.","authors":"Hui Deng, Yuming Wang, Yang Dai, Qian Wang, Hao Lu, Qing Wang","doi":"10.1177/09287329241291323","DOIUrl":"10.1177/09287329241291323","url":null,"abstract":"<p><p>Background As life expectancy increases and the global population ages, the incidence of sarcopenia is also increasing, highlighting the need for better diagnosis and treatment methods.ObjectiveTo study the genetic expression of sarcopenia using bioinformatics methods.MethodsA Weighted Gene Coexpression Network Analysis (WGCNA) was conducted to construct coexpression networks, along with protein-protein interaction networks. Diagnostic biomarker potential was evaluated using receiver operating characteristic curves. An analysis of Single-Sample Gene Set Enrichment Analysis (ssGSEA) was performed in order to determine the amount of immune cell infiltration. We analyzed Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways and Gene Ontology (GO) enrichment using the KEGG.ResultsWGCNA identified modules linked to bone metabolism, ssGSEA showed unique gene enrichment patterns, and 268 genes were found to be differentially expressed in sarcopenia. Fourteen co-expression modules related to bone metabolism were identified, with one showing a strong positive correlation. KEGG pathway analysis indicated downregulation of the renin-angiotensin system and Alzheimer's disease pathways. The differentially expressed genes were primarily involved in adipocyte differentiation.ConclusionThis study analyzes genetic changes and immune cell patterns in sarcopenia, providing insights into its causes and potential diagnostic markers for future research on treatments.</p>","PeriodicalId":48978,"journal":{"name":"Technology and Health Care","volume":"33 2","pages":"1140-1153"},"PeriodicalIF":1.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}