Jove-Journal of Visualized Experiments最新文献_第7页

Laparoscopic Left Approach Resection of the Caudate Lobe. 腹腔镜左入路尾状叶切除术。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-02-28 DOI: 10.3791/67677

Zhoubin Feng, Lei Cai, Jianan Feng, Cheng Zhang, Yuyan Xu, Yaohong Wen, Hongkun Tan, Chunming Wang, Wenxuan Liu, Guolin He, Mingxin Pan, Shunjun Fu

{"title":"Laparoscopic Left Approach Resection of the Caudate Lobe.","authors":"Zhoubin Feng, Lei Cai, Jianan Feng, Cheng Zhang, Yuyan Xu, Yaohong Wen, Hongkun Tan, Chunming Wang, Wenxuan Liu, Guolin He, Mingxin Pan, Shunjun Fu","doi":"10.3791/67677","DOIUrl":"https://doi.org/10.3791/67677","url":null,"abstract":"Laparoscopic caudate lobectomy (LCL) is one of the most challenging types of laparoscopic liver resections. The main difficulty lies in the deep anatomical position of the caudate lobe, which is closely adjacent to the first and second hepatic ports and the inferior vena cava, increasing the risk of major bleeding during surgery. In addition to a thorough understanding of the anatomy of the caudate lobe tumor, comprehensive imaging assessment, and three-dimensional reconstruction, the flexible choice of surgical approach is also key to reducing surgical difficulty and improving safety. We perform laparoscopic caudate lobectomy using a left-sided approach, especially when the tumor is located in the area of the caudate lobe close to the inferior vena cava. This method avoids the step of splitting the liver substance to expose the field of view required by the traditional anterior approach, with the advantages of larger operating space and shorter operation time. At the same time, combined with preoperative three-dimensional reconstruction technology, we have significantly reduced the risk of damaging important blood vessels and increased the success rate of resection of tumors in the caudate lobe.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 216","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1. 荧光法研究结核分枝杆菌与免疫受体SLAMF1的相互作用。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-02-28 DOI: 10.3791/67745

Angela María Barbero, Rodrigo Emanuel Hernández Del Pino, Verónica Edith García, Virginia Pasquinelli

{"title":"Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1.","authors":"Angela María Barbero, Rodrigo Emanuel Hernández Del Pino, Verónica Edith García, Virginia Pasquinelli","doi":"10.3791/67745","DOIUrl":"https://doi.org/10.3791/67745","url":null,"abstract":"The evaluation of direct interaction between pathogens and immune receptors usually involves sophisticated techniques or implies the use of transgenic strains and genetically engineered cells. Here, an alternative method to detect biochemical interaction between the macrophage microbial sensor SLAMF1 and Mycobacterium tuberculosis is described. Two technical approaches employing flow cytometry and fluorescence microscopy were developed. Total cell protein extracts from human macrophages were generated, then incubated with whole cells of M. tuberculosis (WCMtb) or M. tuberculosis antigens (Mtb Ags) overnight at 4 °C and finally cross-linked using formaldehyde/glycine/ethylene glycol bis (succinimidyl succinate) treatment. SLAMF1 interaction with WCMtb by flow cytometry was detected with a PE-specific anti-SLAMF1 antibody. The existence of interaction by fluorescence microscopy was performed by attaching Rhodamine-PE stained Mtb Ags to poly-D-lysine coated slides, which were incubated with the total protein extract from monocyte-derived macrophages. After cross-linking treatment, SLAMF1 was visualized using primary (anti-SLAMF1) and secondary (Alexa Fluor 488) antibodies. The assays provided a strong biochemical tool to measure pathogen-immunoreceptor interactions, overcoming the difficulties associated with transgenic cell lines and protein gene expression modulation experiments.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 216","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of Step-Width Manipulation on Running Biomechanics. 步宽操作对跑步生物力学的影响。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-02-28 DOI: 10.3791/67152

Liu Xu, Yuan Wang, Hanhui Jiang, Xiaoyi Yang, Justin Fernandez, Qichang Mei, Yaodong Gu

{"title":"Influence of Step-Width Manipulation on Running Biomechanics.","authors":"Liu Xu, Yuan Wang, Hanhui Jiang, Xiaoyi Yang, Justin Fernandez, Qichang Mei, Yaodong Gu","doi":"10.3791/67152","DOIUrl":"https://doi.org/10.3791/67152","url":null,"abstract":"Step width is a critical factor influencing lower limb biomechanics during running, significantly affecting stability, performance, and injury risk. Understanding these effects is essential for optimizing running performance and minimizing injury risk. This study evaluated the effects of varying step widths on lower limb biomechanics at different running speeds. Thirteen healthy Chinese males (aged 20-24) participated in the study, running at speeds of 3.0 m/s and 3.7 m/s using six distinct step widths: the preferred step width and five variations (reductions of 13% and 6.5%, and increases of 6.5%, 13%, and 25%, based on leg length). Data were collected using a motion capture system and force plates and analyzed through repeated measures ANOVA and correlation tests. The results indicated that wider step widths significantly reduced peak knee abduction moments and hip adduction angles, whereas narrower step widths increased knee joint loading. These findings have important implications for clinicians and runners, suggesting that careful step width selection can help reduce injury risk and enhance running efficiency. This study contributes a new dataset that lays the foundation for future research into the relationship between step width and running biomechanics and serves as a reference for training and rehabilitation practices.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 216","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laparoscopic Choledochal Cyst Excision and Roux-en-Y Choledochojejunostomy in Adults. 成人腹腔镜胆总管囊肿切除及Roux-en-Y胆总管空肠吻合术。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-02-28 DOI: 10.3791/67553

Haisong Xu, Gongze Peng, Xiaoxia Zeng, Peng Zhang, An Zhang, Wenhao Huang, Yuehua Guo, Tianchong Wu

{"title":"Laparoscopic Choledochal Cyst Excision and Roux-en-Y Choledochojejunostomy in Adults.","authors":"Haisong Xu, Gongze Peng, Xiaoxia Zeng, Peng Zhang, An Zhang, Wenhao Huang, Yuehua Guo, Tianchong Wu","doi":"10.3791/67553","DOIUrl":"https://doi.org/10.3791/67553","url":null,"abstract":"Choledochal cysts (CCs), known as congenital choledochal dilatations, are more prevalent in Asia. The majority of patients with abdominal symptoms are diagnosed and treated during early childhood, which results in a lower prevalence of CCs in adults. The treatment of choice for CCs is complete cyst excision followed by choledochojejunostomy. Laparoscopic surgery is now more widely accepted than traditional open surgery due to its smaller incisions, faster recovery, and less postoperative pain. However, there are few reports on laparoscopic excision of CCs in adults. This article presents a protocol that describes and demonstrates the complete procedure for laparoscopic excision of a choledochal cyst and Roux-en-Y choledochojejunostomy. A 32-year-old woman diagnosed with a 2.5 cm by 3 cm CC underwent surgery using a laparoscopic approach with post-colonic anastomosis. The procedure lasted 290 min with an estimated blood loss of approximately 100 mL. A follow-up abdominal CT scan on the sixth postoperative day showed a satisfactory recovery, leading to her discharge on the ninth day. This study aims to demonstrate the feasibility and safety of laparoscopically assisted excision of CCs in adults. This procedure is expected to become the preferred surgical option for CCs in adults due to its minimal surgical trauma and rapid postoperative recovery.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 216","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue. 活体组织新生血管生成的体外力学特性2.5D模型。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-02-28 DOI: 10.3791/67641

Janine Grolleman, Iris M T Pijnenburg, Carlijn V C Bouten, Vito Conte, Cecilia M Sahlgren

{"title":"2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue.","authors":"Janine Grolleman, Iris M T Pijnenburg, Carlijn V C Bouten, Vito Conte, Cecilia M Sahlgren","doi":"10.3791/67641","DOIUrl":"https://doi.org/10.3791/67641","url":null,"abstract":"Sprouting angiogenesis is the formation of new blood vessels from pre-existing vasculature and is of great importance for physiological such as tissue growth and repair and pathological processes, including cancer and metastasis. The multistep process of sprouting angiogenesis is a molecularly and mechanically driven process. It consists of induction of cellular sprout by vascular endothelial growth factor, leader/follower cell selection through Notch signaling, directed migration of endothelial cells, and vessel fusion and stabilization. A variety of sprouting angiogenesis models have been developed over the years to better understand the underlying mechanisms of cellular sprouting. Despite advancements in understanding the molecular drivers of sprouting angiogenesis, the role of mechanical cues and the mechanical driver of sprouting angiogenesis remains underexplored due to limitations in existing models. In this study, we designed a 2.5D ex vivo model that enables us to mechanically characterize cellular sprouting from a porcine carotid artery using traction force microscopy. The model identifies distinct force patterns within the sprout, where leader cells exert pulling forces and follower cells exert pushing forces on the matrix. The model's versatility allows for the manipulation of both chemical and mechanical cues, such as matrix stiffness, enhancing its relevance to various microenvironments. Here, we demonstrate that the onset of sprouting angiogenesis is stiffness-dependent. The presented 2.5D model for quantifying cellular traction forces in sprouting angiogenesis offers a simplified yet physiologically relevant method, enhancing our understanding of cellular responses to mechanical cues, which could advance tissue engineering and therapeutic strategies against tumor angiogenesis.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 216","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gastroscope-assisted Trans-Trocar Gastrostomy and Abdominal Exploration. 胃镜辅助经套管针胃造口术及腹部探查。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-02-28 DOI: 10.3791/67703

Weidong Feng, Yan Qian, Qingde Wen, Weigang Dai, Shirong Cai

{"title":"Gastroscope-assisted Trans-Trocar Gastrostomy and Abdominal Exploration.","authors":"Weidong Feng, Yan Qian, Qingde Wen, Weigang Dai, Shirong Cai","doi":"10.3791/67703","DOIUrl":"https://doi.org/10.3791/67703","url":null,"abstract":"Advanced endoscopic technologies have revolutionized gastrointestinal surgical procedures, transitioning from open or laparoscopic methods to endoscopic interventions. Gastrostomy is a classic clinical technique for patients with swallowing impediments due to obstructions in the cardiac or esophageal regions and has evolved significantly with the establishment of percutaneous endoscopic gastrostomy (PEG). While PEG offers reduced trauma and swift recovery, it falls short of exploratory capabilities and risks damaging adjacent organs. Therefore, we developed an innovative approach-gastroscope-assisted trans-Trocar gastrostomy (GTTG), which not only takes full play to the advantages of PEG with less trauma and faster postoperative recovery but also takes into account the advantages of laparoscopic gastrostomy with a good exploration of the abdominal cavity. This technique is characterized by ease of operation, minimal trauma (including short operation time and no redundant incision), rapid recovery, the ability to explore the abdominal cavity, and avoidance of injury to adjacent organs, which is quite worthy of widespread promotion in clinical settings.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 216","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterizing Extracellular Vesicles from Biological Fluids. 生物体液中细胞外囊泡的表征

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-02-28 DOI: 10.3791/67699

Brenna S Hefley, Pawan Shrestha, Brenda Vasini, Purnima Sharma, Dimitrios Karamichos

引用次数: 0

A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy. 利用mRNA产生瞬时嵌合抗原受体T细胞的非病毒方法用于癌症免疫治疗。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-02-21 DOI: 10.3791/67548

Liang Hu, Robert Berahovich, Yanwei Huang, Shiming Zhang, Jinying Sun, Xianghong Liu, Hua Zhou, Shirley Xu, Haoqi Li, Vita Golubovskaya, Lijun Wu

{"title":"A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy.","authors":"Liang Hu, Robert Berahovich, Yanwei Huang, Shiming Zhang, Jinying Sun, Xianghong Liu, Hua Zhou, Shirley Xu, Haoqi Li, Vita Golubovskaya, Lijun Wu","doi":"10.3791/67548","DOIUrl":"https://doi.org/10.3791/67548","url":null,"abstract":"Chimeric antigen receptor (CAR) T cell therapy has emerged as a pioneering cancer treatment, achieving unprecedented success in treating certain hematological malignancies such as lymphomas and leukemias. However, as more cancer patients receive CAR-T cell therapies, treatment-associated secondary primary malignancies are increasingly being reported partly due to unexpected CAR transgene insertion, raising serious safety concerns. To address this issue, we describe here a nonviral, non-integrating approach to generate transient CAR-T cells using mRNA. We electroporated T cells with modified mRNA encoding a human epidermal growth factor receptor 2 (HER2)-specific CAR and generated transient HER2-targeted CAR-T cells. The CAR was efficiently expressed on the T cell surface 1 day after electroporation, increased by day 2, and dramatically declined by day 5. The transient CAR-T cells exhibited potent cytotoxicity against HER2-positive SKOV-3 ovarian cancer cells and secreted high levels of IFN-ϒ. This protocol provides a step-by-step guide for developing small-scale transient CAR-T cells without permanent CAR transgene integration, describing detailed procedures for preparation of CAR mRNA, activation and transfection of T cells, assessment of CAR expression, and in vitro analysis of CAR-T cell function. This method is suitable for transient CAR-T cell generation in both preclinical and clinical studies.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 216","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determining Ciliary Function and Membrane Impermeability of the Pseudostratified Lung Airway Epithelium. 测定假层状肺气道上皮纤毛功能和膜不渗透性。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-02-21 DOI: 10.3791/67094

Muhammad Faheem, Masfique Mehedi

{"title":"Determining Ciliary Function and Membrane Impermeability of the Pseudostratified Lung Airway Epithelium.","authors":"Muhammad Faheem, Masfique Mehedi","doi":"10.3791/67094","DOIUrl":"https://doi.org/10.3791/67094","url":null,"abstract":"Differentiating primary lung airway epithelial cells in the air-liquid interface (ALI) is a popular technique to develop a multi-cellular pseudostratified airway epithelium that mimics the apical side of the lung airway. While the differentiation of primary lung airway cells is expected, the assessment of biophysical properties like ciliary function and membrane impermeability provides a quality assessment of the airway epithelium and ensures the reliability of the experiment. Here, we describe a straightforward protocol for the development of multi-cellular pseudostratified airway epithelium in ALI culture and assess two important biophysical properties: ciliary function and membrane impermeability. To determine ciliary function, we captured the ciliary movement of a 4-week differentiated airway epithelium using a high-speed camera attached to an inverted microscope, followed by quantifying ciliary beat frequency (CBF) using the Simon Amon Video Analysis (SAVA) system. We also measured transepithelial electrical resistance (TEER) using a volt-ohm meter to determine the epithelial barrier integrity of the airway epithelium.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 216","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143598023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retrospective Cardiac Gating with A Prototype Small-Animal X-ray Computed Tomograph. 小动物x线计算机断层扫描的回顾性心脏门控。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-02-21 DOI: 10.3791/67803

Richard Taschereau, Arion F Chatziioannou, Shili Xu

{"title":"Retrospective Cardiac Gating with A Prototype Small-Animal X-ray Computed Tomograph.","authors":"Richard Taschereau, Arion F Chatziioannou, Shili Xu","doi":"10.3791/67803","DOIUrl":"https://doi.org/10.3791/67803","url":null,"abstract":"The CrumpCAT is a prototype small-animal X-ray computed tomography (CT) scanner developed at our research institution. The CMOS detector with a maximum frame rate of 29 Hz and similar Tungsten X-ray sources with energies ranging from 50 kVp to 80 kVp are widely used across commercially available preclinical X-ray CT instruments. This makes the described work highly relevant to other institutions, despite the generally perceived wisdom that these detectors are not suitable for gating the high heart rates of mice (~600 beats/min). The scanner features medium- (200 µm) and high- (125 µm) resolution imaging, fluoroscopy, retrospective respiratory gating, and retrospective cardiac gating, with iterative or filtered-back projection image reconstruction. Among these features, cardiac gating is the most useful feature for studying cardiac functions in vivo, as it effectively eliminates image blurring caused by respiratory and cardiac motion. Here, we describe our method for preclinical intrinsic retrospective cardiac-gated CT imaging, aimed at advancing research on in vivo cardiac function and structure analysis. The cardiac-gating method acquires a large number of projections at the shortest practical exposure time (~20 ms) and then retrospectively extracts respiratory and cardiac signals from temporal changes in raw projection sequences. These signals are used to reject projections belonging to the high motion rate inspiration phase of the respiratory cycle and to divide the remaining projections into 12 groups, each corresponding to one phase of the cardiac cycle. Each group is reconstructed independently using an iterative method to produce a volumetric image for each cardiac phase, resulting in a four-dimensional (4D) dataset. These phase images can be analyzed either collectively or individually, allowing for detailed assessment of cardiac function. We demonstrated the effectiveness of both approaches of the prototype scanner's cardiac-gating feature through representative in vivo imaging results.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 216","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143598093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0