Jove-Journal of Visualized Experiments最新文献_第8页

Development of an Innovative LED-based Illumination Device for In Vitro Application of Photodynamic Therapy with Rose Bengal. 一种创新的基于led的体外光动力治疗装置的开发。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-12 DOI: 10.3791/68462

Clément Bouchez, Pascal Deleporte, Grégory Baert, Anthony Lefebvre, Olivier Moralès, Nadira Delhem, Guillaume Paul Grolez, Anne-Sophie Dewalle

{"title":"Development of an Innovative LED-based Illumination Device for In Vitro Application of Photodynamic Therapy with Rose Bengal.","authors":"Clément Bouchez, Pascal Deleporte, Grégory Baert, Anthony Lefebvre, Olivier Moralès, Nadira Delhem, Guillaume Paul Grolez, Anne-Sophie Dewalle","doi":"10.3791/68462","DOIUrl":"https://doi.org/10.3791/68462","url":null,"abstract":"This article describes a new photodynamic therapy (PDT) device for evaluating the efficacy of Rose Bengal (RB)-mediated PDT in vitro. The device, called CELL-LED-550/3, consists of two 3D-printed parts, one generating a 550 nm green light from a single LED (the light source part) and the other distributing this light to a 96-well cell culture plate (the light distributor part). The light source part is controlled by a driver with three different modes, enabling the light distributor part to deliver three different irradiance levels to the bottom exterior surface of the wells: 0.02 mW/cm2, 0.23 mW/cm2, or 0.62 mW/cm2. The light distributor part was designed to illuminate the wells individually and simultaneously. To demonstrate the relevance of the CELL-LED-550/3 device, its ability to induce cell death by RB-mediated PDT on HepG2 hepatocellular carcinoma cells was evaluated. First, HepG2 cells were treated for 2 h with increasing concentrations of Rose Bengal, a photosensitizer with an absorption peak (the highest one) at around 550 nm. The treated cells were then illuminated using the CELL-LED-550/3 device set to its maximum irradiance level at light doses of 0.3 J/cm2, 0.6 J/cm2, and 1.2 J/cm2 (respectively, with illumination times of 8 min and 4 s, 16 min and 8 s, and 32 min and 16 s). Finally, viability was measured using Cell-Titer Glo at 24 h post-PDT. The experimental results show that RB-mediated PDT using the CELL-LED-550/3 device is capable of inducing a decrease in the viability of HepG2 cells, in a manner dependent both on the concentration of Rose Bengal and on the administered dose of light. Based on the proof of concept presented in this article, the CELL-LED-550/3 device adds a further tool to the study of RB-mediated PDT.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145193595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Intelligent Structural Color Hydrogel Patch Integrating Diagnosis and Treatment for the Management of Chronic Osteomyelitis. 一种集诊断和治疗于一体的智能结构彩色水凝胶贴片用于慢性骨髓炎的治疗。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-12 DOI: 10.3791/68662

Xiaoyu Han, Qiaofeng Guo, Xiang Wang, Bingyuan Lin, Yiyang Liu, Haiyong Ren, Kai Huang, Huajun Yu

{"title":"An Intelligent Structural Color Hydrogel Patch Integrating Diagnosis and Treatment for the Management of Chronic Osteomyelitis.","authors":"Xiaoyu Han, Qiaofeng Guo, Xiang Wang, Bingyuan Lin, Yiyang Liu, Haiyong Ren, Kai Huang, Huajun Yu","doi":"10.3791/68662","DOIUrl":"https://doi.org/10.3791/68662","url":null,"abstract":"Chronic osteomyelitis (COM) is a persistent bone infection that is difficult to treat with conventional antibiotics due to issues such as drug resistance and adverse side effects. To address this challenge, we have developed a novel smart structural color hydrogel patch (CPG) for both the diagnosis and treatment of COM. The patch combines methylacrylated chitosan modified with phenylboronic acid (CSMA-PBA) as a scaffold, temperature-responsive GelMA hydrogel as filler, and salicylic acid (SA) for drug delivery. Characterization showed excellent mechanical properties and adhesive strength. Drug release experiments confirmed that CPG effectively responds to pH changes, enabling controlled drug release. CPG also serves as a diagnostic tool by detecting pH and temperature changes at the infection site, assisting in postoperative recovery. Antimicrobial tests showed significant inhibition of bacterial growth, and in vitro experiments demonstrated CPG's ability to promote vascular endothelial growth factor expression while reducing inflammatory markers. The study highlights the multifunctional capabilities of the hydrogel patch, including antimicrobial, anti-inflammatory, and pro-vascular effects, positioning it a promising therapeutic option for chronic osteomyelitis.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145193625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the Proximalized Distal Aortic Arch Anastomosis Site. 近端主动脉弓远端吻合部位的比较。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-12 DOI: 10.3791/68751

Ryaan El-Andari, Sabin J Bozso

{"title":"Comparison of the Proximalized Distal Aortic Arch Anastomosis Site.","authors":"Ryaan El-Andari, Sabin J Bozso","doi":"10.3791/68751","DOIUrl":"https://doi.org/10.3791/68751","url":null,"abstract":"Total arch repair (TAR) has traditionally been performed with a zone 3 distal anastomosis. In recent years, proximalization of the distal anastomosis has been utilized with proposed benefits including an easier distal anastomosis, reduced risk of bleeding, and lower rates of recurrent laryngeal nerve injury. While there have been several comparisons between more proximal distal anastomoses and the traditional zone 3 anastomosis, there have been limited comparisons between proximal anastomoses in zones 0-2. TAR with a frozen elephant trunk (FET) in zone 0 or 1 generally requires debranching of the head vessels proximally or a combination of head vessel bypasses with deployment of the FET across the aortic arch. Select hybrid arch FET devices are available in trifurcated configurations specifically made for a zone 0 distal anastomosis. A zone 2 arch can often be performed with a branched or straight graft and with head vessel anastomoses in the aortic arch. In four studies published to date comparing outcomes following proximal (zone 0/1) or more distal (zone 2) anastomoses, the majority of outcomes, including mortality, stroke, and aortic remodeling, were not significantly different. Rates of major bleeding were more common with a zone 2 distal anastomosis. Proximalization of the distal anastomosis at the time of TAR carries several benefits. Rates of bleeding were lower with a more proximal anastomosis, suggesting improved safety with a zone 0 or 1 anastomosis, without added risk in other metrics.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145193635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rice Body Formation Due to Different Etiologies in the Distal Forearm. 前臂远端不同病因导致的稻体形成。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-12 DOI: 10.3791/68802

Wenming Zhang, Weijie Zhou, Haiying Zhou, Hui Lu, Weitao Chu

{"title":"Rice Body Formation Due to Different Etiologies in the Distal Forearm.","authors":"Wenming Zhang, Weijie Zhou, Haiying Zhou, Hui Lu, Weitao Chu","doi":"10.3791/68802","DOIUrl":"https://doi.org/10.3791/68802","url":null,"abstract":"Rice body formation in the distal forearm is an exceedingly rare clinical entity, often associated with chronic synovitis of varying etiologies. This study investigates four unique cases of forearm synovitis with rice body formation, hypothesizing that diverse underlying causes-rheumatoid arthritis, trauma, and idiopathic inflammation-contribute to this condition, and that surgical intervention ensures symptom resolution and prevents recurrence. The patients (three males, one female; aged 42-76 years) presented with heterogeneous clinical manifestations, including localized swelling, nerve compression symptoms, and restricted joint mobility. Diagnostic MRI revealed characteristic features: T1-weighted isointense masses with T2-weighted high-signal foci containing punctate low signals, aiding differentiation from other synovial pathologies. All patients underwent radical synovectomy with median nerve decompression, followed by histopathological analysis. Results demonstrated chronic granulomatous synovitis without evidence of infection (negative cultures and stains for tuberculosis, fungi, and bacteria). Postoperatively, all patients achieved complete symptom relief, with no recurrence or functional deficits observed during follow-up (9-22 months). Literature review highlighted the rarity of forearm rice bodies and underscored MRI's diagnostic superiority over radiography. The cases underscore that rice body formation, while historically linked to rheumatoid arthritis or tuberculosis, can arise from nonspecific synovial inflammation or trauma. Surgical excision combined with nerve release emerged as the definitive treatment, addressing both mechanical compression and inflammatory burden. This study emphasizes the importance of considering diverse etiologies in forearm synovitis, advocating for early MRI evaluation and tailored surgical management to optimize outcomes. The findings contribute to understanding the multifactorial pathogenesis of rice bodies and reinforce the efficacy of synovectomy in preventing recurrence and restoring function.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145193004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three-dimensional Quantification of Intestinal Mucus Using Whole-mount Tissue Imaging. 利用全贴装组织成像技术对肠道粘液进行三维定量。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-12 DOI: 10.3791/68789

Wei-Lin Chen, Zi-Qi Gu, Zi-Yun Qiu, Yu-Huan Tsai

{"title":"Three-dimensional Quantification of Intestinal Mucus Using Whole-mount Tissue Imaging.","authors":"Wei-Lin Chen, Zi-Qi Gu, Zi-Yun Qiu, Yu-Huan Tsai","doi":"10.3791/68789","DOIUrl":"https://doi.org/10.3791/68789","url":null,"abstract":"Mucus plays a critical role in maintaining intestinal homeostasis by facilitating digestion, forming a barrier against microbes, and regulating immune responses within the gut. Its secretion is modulated by various intrinsic and extrinsic factors, including microbial infections and inflammation. While traditional methods for mucus analysis rely on relative quantification, such as measuring mucus thickness in histological sections, these approaches provide limited insight into the actual volume and spatial distribution of secreted mucus within the intestinal lumen. Here, we present a detailed methodology for the absolute, three-dimensional quantification of mucus using whole-mount intestinal tissues and multi-photon microscopy. This protocol includes the preparation of ligated intestinal loops, treatment with carbamoylcholine chloride as a stimulus for goblet cell activation, tissue fixation, and staining for imaging. Using multi-photon microscopy, we acquire Z-stacked images of the luminal surface. These are processed in Imaris software to quantify the volume and spatial distribution of secreted mucus. We demonstrate that carbamoylcholine chloride robustly induces mucus secretion in both ileal and colonic tissues within 30 minutes. The secreted mucus appears in a sporadic and discontinuous manner throughout the lumen, underscoring the importance of volumetric analysis over traditional two-dimensional approaches. This protocol enables researchers to obtain absolute quantitative data and visualize mucus distribution in situ, offering a powerful tool for studying intestinal mucus dynamics under physiological and pathological conditions.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145193203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia. 四甲草履虫体内邻近生物素化蛋白相互作用研究。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-12 DOI: 10.3791/68504

Bozhidar-Adrian Stefanov, Robin Hogg, Dimitris Themis, Mariusz Nowacki

{"title":"In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia.","authors":"Bozhidar-Adrian Stefanov, Robin Hogg, Dimitris Themis, Mariusz Nowacki","doi":"10.3791/68504","DOIUrl":"https://doi.org/10.3791/68504","url":null,"abstract":"Paramecium is a major model organism for the study of molecular mechanisms involved in programmed genomic rearrangements and transposon elimination during meiotic reproduction. Many details of this process remain enigmatic due to the complexity of the involved molecular machinery and transient protein-protein interactions. Here, we present a method for time-resolved labeling of proteins with biotin located close to a target protein of interest. The method can easily be adapted to different targets since the biotinylation is achieved by injection of a DNA encoding a target protein fused to an engineered biotin ligase. The fusion construct is expressed from the promoter of the endogenous gene, enabling a stage-specific expression profile similar to that of the native protein. Notably, the covalently attached biotin moiety allows for the selective enrichment of the labeled proteins using streptavidin-coated beads. Furthermore, with immunofluorescence staining, we show that efficient labeling requires supplementation of the culture with biotin. The plug-and-play engineered expression vector, combined with the efficient injection procedure demonstrated here, allows for the rapid generation of Paramecium lines expressing a modified protein. The biotinylation procedure demonstrated here can be followed up with mass spectrometry for the identification of the enriched proteins that contain the interacting partners of the target protein. Proximity biotinylation has the potential to simplify and accelerate the discovery of protein-protein interactions in Paramecium and ramp up the efforts to understand its genome editing machinery.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145193647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CFD Simulation of Frost on Horizontal Cold Surfaces. 水平冷面霜的CFD模拟。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-12 DOI: 10.3791/68133

Kailiang Huang, Jiaxing Wei, Xianshi Fang, Guohui Feng, Xuejiao Meng, Guodong Qiu

{"title":"CFD Simulation of Frost on Horizontal Cold Surfaces.","authors":"Kailiang Huang, Jiaxing Wei, Xianshi Fang, Guohui Feng, Xuejiao Meng, Guodong Qiu","doi":"10.3791/68133","DOIUrl":"https://doi.org/10.3791/68133","url":null,"abstract":"Frost formation is a common phenomenon observed across many different fields, including refrigeration, construction, and natural gas processing. However, due to its complex nature, developing an accurate and reliable numerical model remains a significant challenge. Despite previous efforts to tackle this issue, current models still have certain limitations. This paper introduces a modified numerical model for frost formation, developed based on the fundamental mechanisms underlying frost formation. The model utilizes an Eulerian multiphase flow approach coupled with the Lee phase change model. In addition, the approach for determining the maximum frost volume fraction is updated, enabling the model to consider density variations during the frosting process. The model is rigorously validated by comparing it with experimental data on thickness, density, and distribution from various studies. The results indicate that the mean absolute relative deviation (MARD) for frost thickness is 8.97%, while the MARD for density is 16.06%. Furthermore, the frost morphology predicted by the model closely matches the experimental observations reported in the reference.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145193649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dissection of Zebrafish Craniofacial Tissues upon Staining with Alcian Blue. 阿利新蓝染色斑马鱼颅面组织解剖。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-12 DOI: 10.3791/68900

Nandini Bhargava, Sundar Ram Naganathan

引用次数: 0

Differences Between Vestibular Migraine and Meniere's Disease Based on Cochlear-Vestibular Function Assessment: A 6-Year Retrospective Clinical Study. 基于耳蜗-前庭功能评估的前庭偏头痛与梅尼埃病的差异：一项6年回顾性临床研究

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-12 DOI: 10.3791/68803

Ling Chen, Qiong Wei, Lin Zhang, Xiaowu Tang, Yongkang Ou

{"title":"Differences Between Vestibular Migraine and Meniere's Disease Based on Cochlear-Vestibular Function Assessment: A 6-Year Retrospective Clinical Study.","authors":"Ling Chen, Qiong Wei, Lin Zhang, Xiaowu Tang, Yongkang Ou","doi":"10.3791/68803","DOIUrl":"https://doi.org/10.3791/68803","url":null,"abstract":"Audiological and vestibular function tests are the most commonly used noninvasive evaluation methods for vertigo diseases. Preliminary studies have shown that detailed medical history, audiological, and vestibular function evaluation are effective methods to distinguish Meniere's disease and vestibular migraine diseases. This study retrospectively included 503 patients with vestibular migraine and 1,125 patients with Meniere's disease. Patients underwent pure tone audiometry and videonystagmography (VNG), including spontaneous nystagmus testing and the caloric test. In our study, we found that patients with Meniere's disease often exhibit abnormal unilateral weakness, while those with vestibular migraine show changes in vestibular function characterized by labyrinthine hyperactivity. Compared to vestibular migraine patients, Ménière's disease patients are more likely to experience unilateral full-frequency hearing loss. This study systematically compared the vestibular function and audiological characteristics of vestibular migraine and Ménière's disease, providing an evidence-based foundation for clinical differential diagnosis.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145193623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Murine Model of Carotid Aneurysm Formation. 小鼠颈动脉动脉瘤形成模型。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-09 DOI: 10.3791/67872

Keishi Kohyama, Aditya M Mittal, Ali Alattar, Rohit Mantena, Catherine Cao, Seungil Kim, William Wagner, Robert Friedlander, Kamil Nowicki

{"title":"A Murine Model of Carotid Aneurysm Formation.","authors":"Keishi Kohyama, Aditya M Mittal, Ali Alattar, Rohit Mantena, Catherine Cao, Seungil Kim, William Wagner, Robert Friedlander, Kamil Nowicki","doi":"10.3791/67872","DOIUrl":"https://doi.org/10.3791/67872","url":null,"abstract":"Cerebral aneurysms are present in approximately 2%-5% of the population and represent a significant public health concern due to their high rates of mortality and morbidity upon rupture. Despite advancements in research, the development of effective and reproducible animal models remains a challenge. Among the available models, surgically induced aneurysms are widely utilized; however, the technical complexity of these procedures often limits their accessibility. These methods require extensive surgical expertise and are associated with higher rates of intraoperative mortality or inconsistent aneurysm formation, which can compromise the reliability of experimental outcomes. To address these limitations, this video article introduces a simplified surgical procedure for inducing carotid aneurysms in mice, performed in a single session. This streamlined approach has demonstrated an impressive 87% success rate in inducing carotid aneurysms in female mice. By reducing technical challenges and improving reproducibility, this method provides a practical and reliable alternative for cerebral aneurysm research. Its widespread adoption could facilitate standardized studies, accelerate the development of therapeutic strategies, and ultimately advance our understanding of aneurysm pathophysiology.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145193597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0