Jove-Journal of Visualized Experiments最新文献_第8页

Bioreactor Assembly for Continuous Culture of Complex Fecal Communities. 用于复杂粪便群落连续培养的生物反应器组件。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-04-25 DOI: 10.3791/68035

Chancellor McGriff, Rachel C Newsome, Michael Lunin, Raad Z Gharaibeh, Christian Jobin

{"title":"Bioreactor Assembly for Continuous Culture of Complex Fecal Communities.","authors":"Chancellor McGriff, Rachel C Newsome, Michael Lunin, Raad Z Gharaibeh, Christian Jobin","doi":"10.3791/68035","DOIUrl":"https://doi.org/10.3791/68035","url":null,"abstract":"The microbiota, especially bacteria, respond to various environmental exposures such as micro and macronutrients, pharmacological compounds, and inflammatory mediators, which dynamically alter community composition and microbial metabolic output. Understanding how physiological culture conditions affect microbial communities and diversity and their metabolic capacity will contribute important knowledge of their impact on health and diseases. Here, we present a protocol adapted from the template published by Auchtung et al. to create mini-bioreactor arrays that can cultivate complex fecal communities, define bacterial consortiums or single strains under precise conditions, including nutrient availability, temperature, flow rate, pH, and oxygen content. We describe the process for building the mini-bioreactor system, including adaptations to improve limitations in the published protocol. We also discuss the setup of the mini-bioreactor system under anaerobic conditions with the use of MEGA media (adapted from Han et al.), which is a rich media supporting the growth of diverse bacteria. We describe the inoculation of gut humanized mouse fecal samples into the mini-bioreactor system, followed by the establishment of complex community cultures within the mini-bioreactor system, which can be grown under continuous flow with aseptic sampling to monitor community composition. This system is adaptable to dietary changes and other cultural modifications. The techniques described here allow for the characterization of diverse, fastidious fecal communities under dynamic conditions or in response to perturbation in isolation from host-derived factors.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 218","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parameterizing V-notch Weir Equations for Flow Monitoring in a Drainage Control Structure. 排水控制结构流量监测参数化v型缺口堰方程。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-04-25 DOI: 10.3791/67971

Sheela Katuwal, Andrew J Craig, Andrew W Rupiper, Natalia P Rogovska, Gabriel M Johnson, Thomas M Isenhart, Rob W Malone

{"title":"Parameterizing V-notch Weir Equations for Flow Monitoring in a Drainage Control Structure.","authors":"Sheela Katuwal, Andrew J Craig, Andrew W Rupiper, Natalia P Rogovska, Gabriel M Johnson, Thomas M Isenhart, Rob W Malone","doi":"10.3791/67971","DOIUrl":"https://doi.org/10.3791/67971","url":null,"abstract":"Accurate estimation of drainage discharge (flow rate or water volume per unit time) is essential for calculating nutrient loads delivered to surface water and assessing the performance of edge-of-field conservation practices. Determining drainage flow rates requires monitoring the head in a drainage control structure and applying an appropriate equation for the weir installed in the structure. Previous studies that developed calibrated equations for weirs in control structures have produced different equations (i.e., different weir flow equation coefficients) for the same type of weir and control structure size. This study describes procedures for setting up experimental water flow rate and head measurements within a drainage control structure and developing a weir equation for flows contained within a V-notch weir. Additionally, the procedure for obtaining a weir equation for overtopping flows -- when the water level exceeds the top of the V-notch weir -- is outlined. This process involves using the weir equation developed in the laboratory for a V-notch weir and an online tool, the \"Weir Flow Equation Coefficients Calculator\", developed by the authors. The online tool can also generate weir flow equation coefficients for both V-notch-contained and overtopping flows across various sizes of Agri Drain water control structures, even when users lack site-specific flow rate vs. head relationship.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 218","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144055398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Management of Failed Latarjet and Off-tract Hill-Sachs Lesion: Arthroscopy-assisted Remplissage and Open Eden-Hybinette Procedure. 失败的Latarjet和离道Hill-Sachs病变的处理：关节镜辅助下的复位和开放Eden-Hybinette手术。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-04-25 DOI: 10.3791/68382

Chen-Heng Hsu, Chieh-An Chuang, Huan Sheu, Cheng-Pang Yang, Yi-Hsuan Lin, Joe Chih-Hao Chiu

{"title":"Management of Failed Latarjet and Off-tract Hill-Sachs Lesion: Arthroscopy-assisted Remplissage and Open Eden-Hybinette Procedure.","authors":"Chen-Heng Hsu, Chieh-An Chuang, Huan Sheu, Cheng-Pang Yang, Yi-Hsuan Lin, Joe Chih-Hao Chiu","doi":"10.3791/68382","DOIUrl":"https://doi.org/10.3791/68382","url":null,"abstract":"This case report describes a 49-year-old former semi-professional judo player with recurrent right shoulder instability following his first dislocation in 2006. He underwent two unsuccessful arthroscopic Bankart repairs due to improper anchor positioning. In March 2020, an open Latarjet procedure was performed to address his ongoing instability, but he suffered another dislocation in January 2023 after a fall. Physical examination revealed a full range of motion, normal rotator cuff strength, and a positive apprehension test. X-rays showed a broken coracoid bone block, screw displacement, and a significant Hill-Sachs lesion. CT confirmed resorption of the previous coracoid graft and an off-track Hill-Sachs lesion. The patient underwent revision surgery, combining an arthroscopic-assisted Remplissage with an Eden-Hybinette procedure. One year postoperatively, the patient reported improved outcomes. Managing recurrent shoulder instability after failed surgeries is challenging. While the Latarjet procedure has a 10% recurrence rate, revision options such as distal tibial allograft and Eden-Hybinette procedures offer promising results. This case emphasizes the importance of combined surgical approaches and individualized treatment plans, particularly for patients with significant humeral bone loss, to enhance stability and reduce recurrent instability.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 218","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144035780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions. 使用选定组织区域的基于质谱的蛋白质组学的流线型方法。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-04-18 DOI: 10.3791/68076

Dongyoon Shin, Dayeon Lee, Young June Jeon, Kyoungbun Lee, Youngsoo Kim, Junho Park

{"title":"A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions.","authors":"Dongyoon Shin, Dayeon Lee, Young June Jeon, Kyoungbun Lee, Youngsoo Kim, Junho Park","doi":"10.3791/68076","DOIUrl":"https://doi.org/10.3791/68076","url":null,"abstract":"Mass spectrometry (MS)-based proteomics enables comprehensive proteome analysis across a wide range of biological samples, including cells, tissues, and body fluids. Formalin-fixed, paraffin-embedded (FFPE) tissue sections, commonly used for long-term archiving, have emerged as valuable resources for proteomic studies. Beyond their storage benefits, researchers can isolate regions of interest (ROIs) from normal tissue regions through collaborative efforts with pathologists. Despite this potential, a streamlined approach for proteomic experiments encompassing ROI isolation, proteomic sample preparation, and MS analysis remains lacking. In this protocol, an integrated workflow that combines macrodissection of ROIs, suspension trapping-based sample preparation, and high-throughput MS analysis is presented. Through this approach, the ROIs of patients' FFPE tissues, consisting of benign serous cystic neoplasms (SCN) and precancerous intraductal papillary mucinous neoplasms (IPMN) diagnosed by pathologists, were macrodissected, collected, and analyzed, resulting in high proteome coverage. Furthermore, molecular differences between the two distinct pancreatic cystic neoplasms were successfully identified, thus demonstrating the applicability of this approach for advancing proteomic research with FFPE tissues.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 218","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endoscope-assisted Anterior Cervical Discectomy and Fusion for the Treatment of Cervical Spondylotic Myelopathy. 内窥镜辅助颈前路椎间盘切除术和融合治疗脊髓型颈椎病。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-04-18 DOI: 10.3791/68292

Wenjie Iv, Shangju Gao, Can Cao, Kuan Lu, Jingchao Wei, Wenyi Li

{"title":"Endoscope-assisted Anterior Cervical Discectomy and Fusion for the Treatment of Cervical Spondylotic Myelopathy.","authors":"Wenjie Iv, Shangju Gao, Can Cao, Kuan Lu, Jingchao Wei, Wenyi Li","doi":"10.3791/68292","DOIUrl":"https://doi.org/10.3791/68292","url":null,"abstract":"Cervical spondylotic myelopathy (CSM) is a common cause of cervical spinal cord disease. Spinal endoscopy offers surgical advantages such as a magnified view and a water-mediated clear surgical field. This study describes an endoscope-assisted anterior cervical discectomy and fusion (ACDF) procedure. The addition of spinal endoscopy to traditional ACDF surgery magnifies the surgical field and allows for more precise operations, thereby improving surgical safety. Postoperatively, patients experienced significant improvements in neurological function, with no complications such as dysphagia, hematoma, or spinal cord injury. Postoperative imaging revealed that spinal cord compression was completely relieved, with sufficient decompression of the spinal cord and optimal placement of the fusion cage. The clear visual field provided by spinal endoscopy improves the identification of cervical anatomical structures during surgery, effectively reducing the risk of injury to the spinal cord and nerves. Endoscope-assisted ACDF has demonstrated excellent clinical and radiological outcomes in the treatment of CSM.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 218","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144065025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tracking Individual Running Metrics in Mice Using a Voluntary Wheel Running Protocol that Minimizes Social Isolation. 使用自愿轮式跑步协议跟踪小鼠的个人跑步指标，最大限度地减少社会隔离。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-04-18 DOI: 10.3791/67960

Elias Elias, Abigail G White, Autumn E Zeak, Melissa T Manners

{"title":"Tracking Individual Running Metrics in Mice Using a Voluntary Wheel Running Protocol that Minimizes Social Isolation.","authors":"Elias Elias, Abigail G White, Autumn E Zeak, Melissa T Manners","doi":"10.3791/67960","DOIUrl":"https://doi.org/10.3791/67960","url":null,"abstract":"Voluntary wheel running (VWR) in rodents is used to evaluate performance and endurance and to simulate exercise. In contrast to other exercise paradigms that require aversive stimulus to induce active movement, wheel running is voluntary. VWR generally consists of placing a running wheel inside the mouse home cage. However, analyzing exercise parameters can typically only be conducted when mice are individually housed, while overall cage effects can be reported when mice are group-housed. Therefore, the current models force experimenters to choose between measuring individual exercise parameters in single-housed mice or measuring group effects in group-housed mice; depending on the research question and model, this could be limiting. Therefore, the goal of this method is to demonstrate a protocol that assesses VWR in a mouse model by quantifying running parameters in individual mice, with minimal isolation. We report on device design, preoperative preparations, and experiment setup. Results of a 6-week training protocol using this VWR model suggest it provides sufficient stimulus to increase spatial object recognition memory, comparable to the clinical effect of exercise. This simple and inexpensive model facilitates investigation into the effect of VWR on multiple measurements of wheel activity, minimizing factors that could limit or affect experimental outcomes.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 218","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparing Transperitoneal Retroperitoneal Access and Percutaneous Puncture for Lumbar Disc Degeneration Modeling in Rabbits. 经腹膜后入路与经皮穿刺对兔腰椎间盘退变模型的比较。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-04-18 DOI: 10.3791/67513

Li Wenhan, He Wenshan, Lu Qunwen, Ye Jiangnan, Zhang Xin, Zhao Xiaoyan, Yang Xi, Zhou Zhibin, Luo Jian, Feng Yue, Su Chengguo

{"title":"Comparing Transperitoneal Retroperitoneal Access and Percutaneous Puncture for Lumbar Disc Degeneration Modeling in Rabbits.","authors":"Li Wenhan, He Wenshan, Lu Qunwen, Ye Jiangnan, Zhang Xin, Zhao Xiaoyan, Yang Xi, Zhou Zhibin, Luo Jian, Feng Yue, Su Chengguo","doi":"10.3791/67513","DOIUrl":"https://doi.org/10.3791/67513","url":null,"abstract":"This study compares the efficacy of two methods for inducing intervertebral disc degeneration (IVDD) in rabbits: percutaneous and trans-retroperitoneal puncture of the annulus fibrosus. Fifteen healthy male New Zealand White rabbits were randomly assigned to three groups: sham, percutaneous puncture, and trans-retroperitoneal puncture. A comprehensive assessment, including mortality rates, morphological and histological evaluations, radiological imaging, and biomarker analysis, was conducted to ensure an accurate and detailed comparison between the two methods. The results demonstrate that both puncture techniques successfully induced IVDD in the rabbit model. However, the trans-retroperitoneal approach resulted in more pronounced degenerative changes in the intervertebral discs while maintaining a significantly lower mortality rate compared to the percutaneous method. These findings highlight the advantages of the trans-retroperitoneal approach in IVDD modeling. This study provides valuable insights into the establishment of IVDD models and lays a foundation for future investigations into effective treatment strategies for low back pain, ultimately improving patient outcomes.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 218","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144038722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brain-Computer Interface-controlled Upper Limb Robotic System for Enhancing Daily Activities in Stroke Patients. 脑机接口控制上肢机器人系统增强脑卒中患者的日常活动。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-04-18 DOI: 10.3791/67601

Hyunji Kim, Won Kee Chang, Won-Seok Kim, Ji-Hee Jang, Yoon-Ah Lee, Mareike Vermehren, Niels Peekhaus, Annalisa Colucci, Cornelius Angerhöfer, Volker Hömberg, Surjo R Soekadar, Nam-Jong Paik

{"title":"Brain-Computer Interface-controlled Upper Limb Robotic System for Enhancing Daily Activities in Stroke Patients.","authors":"Hyunji Kim, Won Kee Chang, Won-Seok Kim, Ji-Hee Jang, Yoon-Ah Lee, Mareike Vermehren, Niels Peekhaus, Annalisa Colucci, Cornelius Angerhöfer, Volker Hömberg, Surjo R Soekadar, Nam-Jong Paik","doi":"10.3791/67601","DOIUrl":"https://doi.org/10.3791/67601","url":null,"abstract":"This study introduces a Brain-Computer Interface (BCI)-controlled upper limb assistive robot for post-stroke rehabilitation. The system utilizes electroencephalogram (EEG) and electrooculogram (EOG) signals to help users assist upper limb function in everyday tasks while interacting with a robotic hand. We evaluated the effectiveness of this BCI-robot system using the Berlin Bimanual Test for Stroke (BeBiTS), a set of 10 daily living tasks involving both hands. Eight stroke patients participated in this study, but only four participants could adapt to the BCI robot system training and perform the postBeBiTS. Notably, when the preBeBiTS score for each item was four or less, the BCI robot system showed greater assistive effectiveness in the postBeBiTS assessment. Furthermore, our current robotic hand does not assist with arm and wrist functions, limiting its use in tasks requiring complex hand movements. More participants are required to confirm the training effectiveness of the BCI-robot system, and future research should consider using robots that can assist with a broader range of upper limb functions. This study aimed to determine the BCI-robot system's ability to assist patients in performing daily living activities.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 218","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144065020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Genes Analysis and Validation of Disulfideptosis in Rat Myocardial Ischemia-Reperfusion Injury Model. 大鼠心肌缺血-再灌注损伤模型二硫沉积差异基因分析及验证。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-04-18 DOI: 10.3791/68019

Qingxian Tu, Wei Li

{"title":"Differential Genes Analysis and Validation of Disulfideptosis in Rat Myocardial Ischemia-Reperfusion Injury Model.","authors":"Qingxian Tu, Wei Li","doi":"10.3791/68019","DOIUrl":"https://doi.org/10.3791/68019","url":null,"abstract":"Myocardial ischemia-reperfusion injury (MIRI) is an additional injury that occurs during the process of restoring heart tissue blood flow after ischemia-induced injury. MIRI seriously affects the efficacy and short-term and long-term prognosis of reperfusion after myocardial infarction. At present, the mechanism of MIRI is not fully clear. Disulfideptosis is a novel mode of cell death, and the relationship between MIRI and disulfideptosis-related genes (DRGs) expression is still unclear. Firstly, this study explores the differentially expressed genes associated with disulfideptosis in MIRI through bioinformatics analysis. Secondly, by constructing a rat model of MIRI, DRGs were further detected. This study identified 12 related genes, including Myh9, SLC7A11, SLC3A2, Myh7b, ACTB, FLNB, Actn1, Actn4, Flnc, Dbn1 and Pdlim1. Myocardial tissue of rats with MIRI shows obvious pathological damage and apoptosis events. The results of immunohistochemistry indicated that MIRI stimulation increased the expression of GLUT1 protein in myocardial tissue but restricted the expression of F-actin protein. In addition, significant differences in the expression of three proteins were validated using external datasets and MIRI rat models. This study demonstrated that DRGs had significant predictive value in MIRI, providing new prospects for exploring biomarkers and potential therapeutic targets of MIRI.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 218","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144065022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anesthesia-free Heartbeat Measurements in Freely Moving Zebrafish. 自由运动斑马鱼的无麻醉心跳测量。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-04-18 DOI: 10.3791/68145

Alexander Machikhin, Artem Slavin, Anastasia Guryleva, Viacheslav Krylov

{"title":"Anesthesia-free Heartbeat Measurements in Freely Moving Zebrafish.","authors":"Alexander Machikhin, Artem Slavin, Anastasia Guryleva, Viacheslav Krylov","doi":"10.3791/68145","DOIUrl":"https://doi.org/10.3791/68145","url":null,"abstract":"Zebrafish (Danio rerio) is a widely used model organism in physiological, pharmacological, and toxicological research due to its genetic similarity to humans and transparent embryonic stage, which facilitates non-invasive cardiovascular studies. However, current methods for heart rate assessment in zebrafish often rely on anesthesia to immobilize the subject, introducing physiological alterations that compromise data accuracy and reproducibility. This study presents a novel, anesthesia-free technique for measuring heartbeat in freely moving zebrafish larvae, addressing a critical limitation in cardiovascular research. The proposed approach integrates shortwave-infrared imaging with machine-learning-based heart tracking, allowing for precise and continuous cardiac activity monitoring in non-immobilized specimens. A convolutional neural network was trained to detect the heart region, and a photoplethysmographic signal was extracted from image sequences to determine heart rate. Experimental validation demonstrated the method's reliability and consistency across multiple test conditions. A key benefit of the methodology is its ability to preserve the natural physiological state of zebrafish, minimizing stress-induced artifacts. This non-invasive, label-free technique offers significant advantages for studying cardiovascular physiology, drug cardiotoxicity, and environmental toxicology, expanding the potential applications of zebrafish as a model for biomedical research.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 218","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144058108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0