Jove-Journal of Visualized Experiments最新文献

{"title":"Salvianolic Acid B Suppresses Epithelial-Mesenchymal Transition-Associated Phenotypes in Cervical Cancer Cells via GPIb Alpha/LYN/TWIST1 Signaling.","authors":"Huiling Yang, Yitong Du, Yichen Feng, Qiaoqiao Zhan, Tingting Zhu, Zejun Wu, Yali Zhuang","doi":"10.3791/72216","DOIUrl":"https://doi.org/10.3791/72216","url":null,"abstract":"<p><p>Cervical cancer remains a major gynecological malignancy, and the epithelial-mesenchymal transition is associated with tumor progression. Salvianolic acid B, a water-soluble polyphenolic compound derived from Salvia miltiorrhiza, has antitumor potential in several cancers, but its role in cervical cancer-related epithelial-mesenchymal transition signaling remains incompletely understood. HeLa and SiHa cervical cancer cells were treated with salvianolic acid B, alone or combined with modulation of platelet glycoprotein Ib alpha or Src-family kinase signaling. Cell viability, wound closure, and proliferative activity were assessed by Cell Counting Kit-8, wound healing, and 5-ethynyl-2'-deoxyuridine incorporation assays. Epithelial-mesenchymal transition markers and LYN/TWIST1 signaling components were analyzed by immunofluorescence and Western blotting. Platelet glycoprotein Ib alpha was modulated by plasmid-mediated overexpression or small interfering RNA-mediated knockdown. Salvianolic acid B reduced cervical cancer cell viability, wound closure, and 5-ethynyl-2'-deoxyuridine-positive staining, decreased N-cadherin and Vimentin, and increased E-cadherin expression. It also downregulated platelet glycoprotein Ib alpha, reduced LYN phosphorylation, and TWIST1 expression. Platelet glycoprotein Ib alpha overexpression partially attenuated these changes; knockdown enhanced them. PP2, a non-selective Src-family kinase inhibitor, also reduced TWIST1 expression and 5-ethynyl-2'-deoxyuridine-positive staining. Salvianolic acid B suppresses cervical cancer cell viability, wound closure, proliferative activity, and molecular changes associated with the epithelial-mesenchymal transition, in part through modulation of a proposed platelet glycoprotein Ib alpha/LYN/TWIST1 signaling axis. Because wound closure was measured under conditions in which viability and proliferation were also reduced, wound healing data should not be interpreted as independent evidence of impaired migration. Further preclinical validation is warranted.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bayesian Predictive Inference for Future Order Statistics from the Two-Parameter Lomax Distribution under Type-II Censoring.","authors":"Omar A Alhelali, A El-Dali, M Yusuf","doi":"10.3791/72654","DOIUrl":"https://doi.org/10.3791/72654","url":null,"abstract":"<p><p>This study develops Bayesian predictive procedures for future order statistics from the two-parameter Lomax distribution under Type-II censored sampling schemes. The proposed framework combines predictive likelihood inference with Bayesian prediction based on independent gamma priors and Markov Chain Monte Carlo (MCMC) simulation using the Metropolis-Hastings algorithm. Bayesian point predictors are derived under the squared error, LINEX, and general entropy loss functions, and predictive intervals are constructed from posterior predictive samples. A detailed Monte Carlo simulation study compares the proposed methods with the classical predictive maximum-likelihood approach across varying sample sizes and censoring levels. For the small-sample scenario (n = 20, r = 14), the Bayesian-LINEX predictor reduced the mean squared prediction error (MSPE) from 0.1542-0.1182, decreased the average predictive interval width (APIW) from 2.4512-2.1985, and increased the coverage probability (CP) from 0.938-0.952 compared with the predictive maximum likelihood method. Overall, the Bayesian methods consistently outperformed the classical approach across all simulation settings, with the Bayesian-LINEX predictor achieving the best predictive performance. These findings demonstrate that the proposed Bayesian framework provides a more accurate and consistent approach for predicting future order statistics from the Lomax distribution while accounting for parameter uncertainty.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological Characteristics of Venous Thromboembolism and Associated Indicators for Catheter-Related Thrombosis Subtypes.","authors":"Yumeng Yuan, Jiaxin Chen, Yali Li","doi":"10.3791/72591","DOIUrl":"https://doi.org/10.3791/72591","url":null,"abstract":"<p><p>Venous thromboembolism (VTE) is a severe and life-threatening complication in hospitalized patients, and central venous catheter (CVC) insertion significantly increases the risk of catheter-related thrombosis (CRT). Epidemiological profiles of hospitalized patients with confirmed VTE are characterized; indicators correlated with CRT subtype are identified; the discriminative performance of circulating biomarkers is evaluated; and VTE recurrence metrics are contrasted between cohorts with and without CVC placement. A single-center retrospective case-case comparative analysis is conducted on 862 patients with imaging-confirmed VTE. Analytical methodologies incorporate univariate and multivariate logistic regression, Kaplan-Meier survival analysis, and receiver operating characteristic (ROC) curve testing. The in-hospital VTE incidence was 2.1%, concentrated in elderly, malignancy, and postoperative patients. CRT incidence was 10.2%, highest with peripherally inserted central catheter (PICC). Five clinical indicators correlated with CRT subtype were identified: prolonged catheter indwelling, catheter malposition, elevated D-dimer levels, malignant tumor history, and prior VTE events. The combination of D-dimer and catheter indwelling time exhibited robust discriminative power (AUC = 0.82) for differentiating CRT from non-CRT VTE among patients with confirmed VTE. The 6-month VTE recurrence rate was 28.4% in the CVC group and 9.7% in the non-CVC group. These observational findings yield only tentative clinical hypotheses aimed at distinguishing thrombotic subtypes among VTE patients. Further multicenter prospective research is necessary to validate these indicators and develop mature, standardized prevention protocols for broad clinical application.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Flow Cytometry-Based Method for Profiling Immune Cells in Human Blood and Tumors.","authors":"Haojie Wang, Cairu Guo, Yujie Niu, Xiangyu Ma, Wen Lin, Junxiao Liu, Chuang Wang, Youfeng Ma, Dian Zhang, Dong Wei, Ge Zhang, Haitao Zhou, Yue Zhang","doi":"10.3791/73373","DOIUrl":"https://doi.org/10.3791/73373","url":null,"abstract":"<p><p>Flow cytometry enables the simultaneous characterization of multiple immune-cell populations and their phenotypic subsets in clinical specimens. However, comprehensive immunophenotyping of limited blood and tumor samples can be challenging because of limited cell availability, sample-processing requirements, and the need for standardized staining and gating procedures. Here, we describe a 12-color full-spectrum flow cytometry method for profiling major immune-cell populations in human peripheral blood and tumor tissue. The panel comprises 11 antibody markers and DAPI and is designed to identify major lymphoid and myeloid populations, including T cells, B cells, NK cells, monocytes, and granulocyte populations, with further characterization of selected cell populations based on marker expression. The workflow includes sample preparation, antibody titration, multicolor staining, viability assessment, spectral reference controls, spectral unmixing, and hierarchical gating. The protocol was optimized for relatively small clinical specimens and can be applied to both whole blood and enzymatically dissociated tumor tissue. Representative data demonstrate the ability of the panel to resolve distinct immune-cell subset distributions in individual blood and paired tumor/peri-tumor specimens. This workflow provides a practical approach for multiparameter immune profiling of limited human blood and tumor specimens and can serve as a basis for subsequent studies requiring more extensive cohort-level validation.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neutrophil Extracellular Traps as Predictive Biomarkers in Trastuzumab-Treated HER2-Positive Breast Cancer: A Prospective Cohort Study.","authors":"Yu Wang, LiZhe Wang, Lingling Zhao, Li Zhou, HaoHao Chen, Yang Zhang, ShenTing Liu, Ting Zhu","doi":"10.3791/72405","DOIUrl":"https://doi.org/10.3791/72405","url":null,"abstract":"<p><p>Neutrophil extracellular traps (NETs) have been implicated in cancer progression and therapy resistance, but their value as predictive biomarkers in HER2-positive breast cancer remains unclear. In this prospective cohort study, we measured circulating myeloperoxidase-DNA (MPO-DNA) complexes as a surrogate marker of NETs in patients with HER2-positive breast cancer receiving adjuvant trastuzumab-based therapy. Serial blood samples were collected at baseline and during treatment, and patients were classified as having progressive disease (PD) or disease control (DC). Baseline NETs, CEA, and CA15-3 levels were significantly higher in patients with PD compared to those with DC. Longitudinal analysis revealed a significant Group × Time interaction, with NETs decreasing in the DC group but increasing in the PD group over the course of treatment. In Firth-penalized logistic regression, higher baseline NETs were independently associated with increased odds of progression (OR per 1-U/L increase, 1.026; 95% CI, 1.004-1.048; p = 0.023). An exploratory NETs cutoff of 97.32 U/L stratified patients into low- and high-NETs groups with significantly different progression-free survival (log-rank p = 0.045); continuous NETs remained associated with shorter PFS in multivariable Cox models adjusted for CEA and CA15-3 (HR per 1-U/L increase, 1.018; 95% CI, 1.003-1.033; p = 0.021). Bootstrap internal validation yielded an optimism-corrected C-index of 0.739 (95% CI, 0.613-0.866). Adding CEA and CA15-3 to NETs improved risk reclassification (NRI = 0.931; p = 0.018) and discrimination (IDI = 0.146; p < 0.001), although the increase in AUC was not statistically significant by DeLong's test. Tissue MPO fluorescence correlated with circulating MPO-DNA even after controlling for disease group. These findings suggest that NETs are associated with trastuzumab efficacy and may serve as adjunctive biomarkers for risk stratification, but the modest sample size, exploratory cutoffs, and single-center design warrant validation in larger prospective cohorts before any clinical application.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptive Dual-Attention DenseNet for Object-Aware Two-Stage Industrial Defect Detection.","authors":"Jiandong Liu, Lina Qi","doi":"10.3791/73920","DOIUrl":"https://doi.org/10.3791/73920","url":null,"abstract":"<p><p>Industrial visual anomaly detection is crucial in intelligent manufacturing to achieve automated quality inspection with low dependency on manual inspection. Nevertheless, traditional single-stage deep learning methods struggle to detect both object classes and fine-grained anomalies across various industrial products. Furthermore, most existing channel attention mechanisms rely on a single attention type, limiting their ability to capture both global contextual information and local channel dependencies. In this paper, we present an Adaptive Dual-Attention Network (ADA-Net) for high-shot, two-stage, supervised industrial anomaly detection. The framework has a hierarchical architecture of two consecutive stages - Stage I for multi-class object classification and Stage II for binary object anomaly detection. The backbone network for feature extraction in both stages is pretrained DenseNet121. We provide an Adaptive Dual-Attention (ADA) module that uses a globally learned scalar fusion coefficient to combine Efficient Channel Attention (ECA) and Squeeze-and-Excitation (SE) representations for improved feature representation. ADA-Net is tested on the VisA dataset using the conventional 2cls_highshot supervised setup. It employs the weighted cross-entropy loss function, adaptive learning-rate scheduling, and early stopping in training. Experiment results show that our proposed ADA-Net can reach 100% Accuracy, Recall, Precision, ROC-AUC, F1-score, Cohen's Kappa, and MCC in Stage I, and Stage II results include 97.11% Accuracy, 92.84% Precision, 92.45% Recall, 92.64% F1-score, 98.00% ROC-AUC, 0.8529 MCC, and 0.8529 Cohen's Kappa. Ablation experiments and qualitative Grad-CAM analysis support the validity and explainability of the suggested framework on the evaluated VisA benchmark.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Utero Valproic Acid Exposure in Cryptorchidism Reports: Integrating FAERS, Toxicogenomics, Transcriptomics, and Mendelian Randomization.","authors":"Zhanbo Wang, Jinxin Yuchi, Hanbao Gu, Yandong Niu, He Huang, Ran Tao, Xiaoqing Xu, Guangcheng Yang","doi":"10.3791/72268","DOIUrl":"https://doi.org/10.3791/72268","url":null,"abstract":"<p><p>To screen the FDA Adverse Event Reporting System (FAERS) for valproic acid (VPA)-associated cryptorchidism reports, restrict developmental interpretation to documented transplacental/in utero exposure, and prioritize candidate genes without claiming mechanistic validation, we analyzed 7,232 deduplicated primary-suspect VPA reports in males aged ≤18 years from 2004Q1-2025Q4. Cryptorchidism screening identified 108 reports; exposure-route review defined the prenatal subset. Disproportionality analyses used pediatric male FAERS reports with primary-suspect drugs as the background. Curated human VPA-associated genes from the Comparative Toxicogenomics Database (CTD) were intersected with differentially expressed genes from cryptorchidism testicular tissue (GSE16191). The 21 shared genes underwent two-sample Mendelian randomization (MR) using GTEx v8 testis eQTL instruments and FinnGen cryptorchidism data (261 cases and 374,410 controls). Exposure route was available for 104 reports; 93 (89.42%) documented transplacental/in utero exposure, whereas 11 other/non-in utero reports and four reports with missing route information were excluded from developmental interpretation. GPX7 and PLCL1 showed nominal inverse-variance-weighted associations, but neither survived Benjamini-Hochberg correction (both q = 0.399) or Bonferroni correction (adjusted p = 0.693 and 0.798, respectively). ACYP2 showed a borderline inverse association. Sensitivity estimators did not consistently support these signals. FAERS identified a VPA-cryptorchidism reporting signal, but cannot estimate incidence or establish causality. Prenatal interpretation was limited to 93 in utero reports. The integrated CTD, transcriptomic, and MR findings support exploratory candidate prioritization rather than a validated causal or mechanistic chain.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Visualized Empowerment Nursing Pathway for Nutritional Outcomes in Lymphoma with Chemotherapy-Induced Oral Mucositis: A Retrospective Cohort Study.","authors":"Aijun Wu, Wenjun Wang, Yun Yin","doi":"10.3791/72527","DOIUrl":"https://doi.org/10.3791/72527","url":null,"abstract":"<p><p>This study examined the effect of an empowerment nursing model on the nutritional status of patients with lymphoma who developed oral mucositis during chemotherapy and further developed a visualized implementation pathway. A total of 133 patients with lymphoma who received chemotherapy and developed oral mucositis between March 2024 and October 2025 were included. Based on previous nursing records, 62 patients who had received an empowerment nursing model centered on \"assessment-identification-goal setting-training-implementation-feedback-continuing management\" were assigned to the study group, while 71 patients who had received routine nursing were assigned to the control group. After baseline adjustment, the study group showed more favorable Patient-Generated Subjective Global Assessment (PG-SGA) and Nutritional Risk Screening (NRS) 2002 scores, body weight, body mass index (BMI), serum nutritional markers, dietary intake, self-management, symptom burden, and quality-of-life outcomes than the control group, while the change in oral mucositis grade and the visual analog scale (VAS) pain score also favored the study group. After false-discovery-rate correction, ulcer duration, mucosal healing time, and length of hospital stay remained shorter in the study group, whereas the differences in malnutrition rate, oral nutritional supplement use, and medication-use duration were no longer significant. These findings suggest that the visualized empowerment nursing pathway was associated with better nutritional status and oral intake, faster recovery from oral mucositis, and stronger self-management ability.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis and Improvement of Factors Affecting Satisfaction of Patients with Postoperative Patient-Controlled Analgesia.","authors":"Wei Liu, Chao Liu, Weixin Zhang, Bailin Jiang, Miao He","doi":"10.3791/73348","DOIUrl":"https://doi.org/10.3791/73348","url":null,"abstract":"<p><p>Patient-controlled analgesia (PCA) is widely used for postoperative pain management, yet patient satisfaction remains influenced by multiple clinical and educational factors. Identifying modifiable determinants may help optimize postoperative analgesic care. To evaluate postoperative pain management and satisfaction with postoperative PCA, identify factors associated with patient satisfaction, and propose improvement strategies. A total of 887 patients who underwent surgery and received postoperative PCA between January 2023 and April 2024 were retrospectively enrolled. Satisfaction was assessed using a 5-point Likert scale, and patients were classified into high-satisfaction (very satisfied/satisfied) and low-satisfaction (neutral/dissatisfied/very dissatisfied) groups. Clinical characteristics were compared, and independent predictors of low satisfaction were identified using multivariable logistic regression. Internal validation was performed using 5,000 nonparametric Bootstrap resamples. Among 887 patients, 852 (96.05%) reported high satisfaction, and 35 (3.95%) low satisfaction. Moderate-to-severe pain, insufficient preoperative communication, inadequate postoperative follow-up education, poor PCA awareness, and lower satisfaction with PCA button use were more common in the low-satisfaction group (all p <0.05). Multivariable analysis identified moderate-to-severe pain (Odds ratio (OR) = 2.704, 95% confidence interval (CI): 1.158-6.319), insufficient preoperative communication (OR = 2.078, 95% CI: 1.002-4.310), poor PCA awareness (OR = 3.062, 95% CI: 1.164-8.052), and inadequate postoperative education (OR = 3.300, 95% CI: 1.263-8.621) as independent predictors of low satisfaction. Satisfaction with PCA button use was significant only in univariate analysis. Bootstrap validation demonstrated generally consistent effect directions, although confidence intervals for several variables remained wide. Pain intensity, preoperative communication, postoperative education, and PCA awareness are key determinants of postoperative PCA satisfaction. Improving perioperative patient education, communication, and pain control may enhance patient experience and optimize postoperative pain management.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traditional Chinese Medicine as Adjunctive Care in Cancer and Rheumatoid Arthritis: A Narrative Review of Shared Frameworks and Evidence Gaps.","authors":"Boyuan Li, Xue Xu","doi":"10.3791/73941","DOIUrl":"https://doi.org/10.3791/73941","url":null,"abstract":"<p><p>Traditional Chinese medicine (TCM) has increasingly been investigated as an adjunctive approach to conventional treatment in chronic and complex diseases, including cancer and rheumatoid arthritis (RA). Although cancer and RA differ substantially in pathogenesis and therapeutic goals, both are associated with persistent symptom burden, treatment-related adverse effects, impaired physical function, psychological distress, and long-term supportive-care needs. This narrative review evaluates TCM within this shared adjunctive-care framework while maintaining disease-specific interpretation of the available evidence. In cancer, TCM-related interventions have been investigated for supportive symptom management, pain relief, treatment tolerance, quality-of-life improvement, and potential modulation of biological processes, including apoptosis, angiogenesis, immune regulation, invasion, metastasis, and multidrug resistance. In RA, Chinese herbal medicines, acupuncture, and related approaches have been evaluated for their potential effects on pain, inflammation, physical function, bone remodeling, and early disease-related processes. TCM-related mind-body practices, such as qigong and tai chi, may also provide psychological support, whereas mindfulness-based interventions are considered supportive-care strategies rather than TCM interventions. Current evidence ranges from mechanistic and preclinical studies to observational studies, single-arm clinical studies, randomized controlled trials, systematic reviews, and meta-analyses, with substantial variation in methodological quality. Overall, TCM should be considered a potential complement to, rather than a replacement for, established oncological or rheumatological treatment. Further well-designed clinical studies, standardized interventions, rigorous quality control, safety monitoring, and clinically meaningful outcome assessment are required to define its appropriate role in multidisciplinary care.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149167802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}