Jove-Journal of Visualized Experiments最新文献

{"title":"Qi-Fu-Yin Attenuates Frailty by Regulating Gut Microbiota and Neuroendocrine Hormones in 5xFamilial Alzheimer's Disease Transgenic Mice.","authors":"Xiuzhao Yang, Tianyuan Ye, Yun He, Junqi Zhao, Jing Wang, Xiaorui Cheng","doi":"10.3791/73003","DOIUrl":"https://doi.org/10.3791/73003","url":null,"abstract":"<p><p>Frailty is a common and debilitating feature of Alzheimer's disease (AD), yet effective therapeutic strategies targeting both cognitive decline and physical frailty remain limited. Qi-Fu-Yin (QFY), a traditional Chinese herbal formulation, has been used to treat aging-related disorders, but its effects on frailty in AD and the underlying mechanisms are unclear. This study aimed to evaluate whether QFY alleviates frailty in 5xFamilial Alzheimer's Disease (FAD) transgenic mice and to explore the potential involvement of gut microbiota and neuroendocrine hormones. Mice were treated with QFY at low (2.8 g/kg), medium (5.6 g/kg), or high (11.2 g/kg) doses, or with donepezil as a positive control, for 194 days. Frailty-related behaviors were assessed using CatWalk gait analysis, grip strength, rotarod, nest-building, and aging scoring. Plasma hormone levels were measured, and fecal gut microbiota composition and function were analyzed by 16S rRNA sequencing and metagenomic analysis. QFY treatment significantly improved gait parameters, grip strength, motor coordination, nesting ability, and reduced aging scores in 5xFAD mice, with effects comparable to or superior to those of donepezil. QFY also restored aberrant levels of estradiol, testosterone, and corticosterone, and reshaped gut microbiota composition, particularly by increasing the abundance of Adlercreutzia, which negatively correlated with senescence and testosterone levels. Functional metagenomics revealed that QFY partially restored disease-associated microbial pathways, including those involved in carbohydrate metabolism and virulence. These findings suggest that QFY alleviates frailty in 5xFAD mice, with effects associated with concurrent modulation of gut microbiota and neuroendocrine function, though causal relationships require further validation. These findings support further investigation of QFY as a potential intervention for AD-associated frailty.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149167259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Early Stepwise Rehabilitation Nursing with Wrist Function and Pain Outcomes After Wrist Arthroscopy: A Retrospective Cohort Study.","authors":"Lili Men, Xin Liu, Lulu Wei, Wei Zhao, Pengcheng Xu, Xing Wu","doi":"10.3791/73804","DOIUrl":"https://doi.org/10.3791/73804","url":null,"abstract":"<p><p>Wrist arthroscopy is widely used as a minimally invasive treatment for wrist disorders. Postoperative rehabilitation is an important component of functional recovery. Conventional rehabilitation often follows a relatively uniform schedule and relies primarily on pharmacological pain management; consequently, stage-specific and individual needs may not be fully addressed. This single-center retrospective cohort analysis compared observed postoperative outcomes between patients receiving early stepwise rehabilitation care and those receiving the traditional rehabilitation model. The intervention group (n = 94) adopted a phased rehabilitation plan in which training intensity was dynamically adjusted according to the Numeric Rating Scale (NRS), with additional physical therapy, psychological support, and family participation. The conventional group received conventional rehabilitation. At 3 and 6 months postoperatively, the intervention group showed higher Modified Mayo Wrist Score (MMWS), greater range of motion (ROM), stronger grip strength, and lower NRS scores than the conventional group (P < 0.001). Postrehabilitation complications were numerically less frequent in the intervention group than in the conventional group, but the difference was not statistically significant (6.4% vs. 15.3%, P = 0.080). The intervention group also showed higher postrehabilitation sensory conduction velocity (SCV), motor conduction velocity (MCV), and sensory nerve action potential (SNAP) amplitude, shorter two-point discrimination distance, lower Self-Rating Anxiety Scale (SAS) and Self-Rating Depression Scale (SDS) scores, higher rehabilitation compliance, and higher satisfaction than the conventional group (P < 0.05). Early stepwise rehabilitation was associated with more favorable functional, pain, neurophysiological, sensory, psychological, adherence, and satisfaction outcomes; however, given the single-center retrospective design and non-random treatment allocation, these findings should be interpreted as associations rather than evidence of causal effectiveness.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149169548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Value Stream Mapping-Guided Optimization of Enhanced Recovery After Surgery Nursing in Geriatric Orthopedics: A Controlled Study.","authors":"Chuochuo Yu, Mingyang Zhao, Guoquan Chen, Ruixin Qiu, Yufan Bu, Jianguo Huang, Yue Nie, Ziping Zhao, Chuliang Yao","doi":"10.3791/71940","DOIUrl":"https://doi.org/10.3791/71940","url":null,"abstract":"<p><p>This study investigated whether value stream mapping (VSM)-guided optimization of enhanced recovery after surgery (ERAS) nursing could improve nursing efficiency and reduce waiting time in geriatric orthopedic patients. A non-synchronous controlled study included 105 elderly orthopedic inpatients. The control group (n = 53) received conventional ERAS nursing, whereas the experimental group (n = 52) received an optimized nursing package developed after VSM-based workflow diagnosis, including centralized preoperative examination scheduling, standardized process management, visual dashboards, multidisciplinary collaboration, and structured materials management. Nursing efficiency, waiting time, early postoperative recovery indicators, complications, and satisfaction were compared. Process efficiency was higher in the experimental group than in the control group (15.15% ± 1.71% vs 8.01% ± 1.34%, p < 0.001). Total waiting time was shorter in the experimental group (12.54 ± 2.36 h vs 28.57 ± 4.45 h, p < 0.001). Preoperative preparation time, first ambulation time, and hospital stay were also reduced, and the 72 h pain score, first flatus time, joint range of motion, and satisfaction improved. Value stream mapping-guided ERAS nursing optimization may improve perioperative process efficiency and early recovery in geriatric orthopedic patients.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149171116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Notch Receptor 2 Drives Epithelial-Mesenchymal Transition and M2-Like Macrophage Polarization in Human Colorectal Cancer Tissues and HCT116 Cells.","authors":"Xiaobin Lin, Shuyi Wang, Min Sun, Chunxiao Zhang, Chen Wei, Chaogang Yang, Rongzhang Dou, Qing Liu, Bin Xiong","doi":"10.3791/72652","DOIUrl":"https://doi.org/10.3791/72652","url":null,"abstract":"<p><p>Despite advances in understanding colorectal cancer (CRC) biology, how tumor-cell signaling coordinates epithelial-mesenchymal transition (EMT) with macrophage polarization remains incompletely defined. We investigated whether NOTCH2 expression is associated with EMT and with a GATA3/IL-4-dependent macrophage-polarizing phenotype in CRC. NOTCH2 expression and prognostic associations were analyzed using TCGA data and validated in 20 paired CRC and adjacent normal tissue (ANT) specimens. Gain- and loss-of-function experiments in HCT116 cells assessed proliferation, migration, invasion, EMT markers, GATA3, and IL-4. A Transwell co-culture system containing transfected HCT116 cells and peripheral blood mononuclear cells (PBMCs) was used to evaluate CD206-positive M2-like macrophage polarization, and IL-4 neutralization was used to test the contribution of IL-4. NOTCH2 was increased in CRC tissues, and higher NOTCH2 expression was associated with poorer overall survival. NOTCH2 overexpression increased CRC-cell proliferation, migration, invasion, Vimentin, GATA3, and IL-4, while reducing E-cadherin; NOTCH2 silencing produced the opposite changes. Clinical tissues showed increased NOTCH2, GATA3, IL-4, CD163, and Vimentin, together with reduced E-cadherin. In co-culture, NOTCH2 overexpression increased the proportion of CD206-positive M2-like cells, whereas NOTCH2 knockdown reduced it. IL-4 neutralization significantly attenuated the increase in CD206-positive cells induced by NOTCH2 overexpression. These findings support associations among NOTCH2-associated malignant phenotypes, EMT, altered GATA3/IL-4 expression, and IL-4-dependent M2-like macrophage polarization in CRC, while direct transcriptional regulation of the GATA3/IL-4 axis remains to be established.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149171387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the Role of miR-93-5p in Children Infected by Mycoplasma pneumoniae with Digestive System Injury.","authors":"Pengyi Wu, Fanghua Jiang, Jinmei Xu, Tianyi Zhu","doi":"10.3791/72281","DOIUrl":"https://doi.org/10.3791/72281","url":null,"abstract":"<p><p>MiR-93-5p has been implicated in modulating acute lung injury, but its role and diagnostic value in pediatric Mycoplasma pneumoniae pneumonia (MPP) with digestive system injury remain unclear. This study evaluated the role of miR-93-5p as a potential diagnostic biomarker for MPP and associated digestive complications in children. Clinical samples were collected from pediatric patients with MPP and healthy controls. MiR-93-5p expression was quantified by reverse transcription quantitative PCR. Mycoplasma pneumoniae (MP)-infected A549 cells were used to establish a cellular MPP model to evaluate changes in cell viability and pro-inflammatory cytokines. Toll-like receptor 4 (TLR4) targeting by miR-93-5p was assessed using a luciferase reporter assay. Diagnostic performance was evaluated using receiver operating characteristic (ROC) curve analysis. MiR-93-5p levels were significantly decreased in patients with MPP compared with healthy controls (P < 0.001) and were further decreased in patients with MPP with digestive system injury compared with those with MPP alone. MP infection reduced cell viability and increased IL-6 and TNF-α levels, whereas miR-93-5p overexpression attenuated these effects. TLR4 expression was increased following MP infection and negatively regulated by miR-93-5p. Clinically, digestive system injury in patients with MPP was associated with lower miR-93-5p levels. ROC curve analyses showed favorable diagnostic performance for distinguishing patients with MPP from healthy controls and patients with MPP with digestive system injury from those with MPP alone. MiR-93-5p directly targets TLR4 in pediatric MPP, and its downregulation may contribute to disease progression and associated digestive system injury. These findings suggest that miR-93-5p may serve as a potential diagnostic biomarker for MPP and MPP with digestive system injury.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149166708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxford Nanopore Long-Read Sequencing of Bat Guano and Urine for Microbiome Characterization and Pathogen Detection.","authors":"Abhranil Gangopadhayya, Pilot D Dovih, Kritika M Garg, Balaji Chattopadhyay","doi":"10.3791/70544","DOIUrl":"https://doi.org/10.3791/70544","url":null,"abstract":"<p><p>Deoxyribonucleic acid (DNA) sequencing has made possible the study of genetic sequences for taxonomic assignment, mutational analyses, and novel organism discovery. Long-read sequencing methods offer advantages over short-read methods because they can sequence entire DNA molecules without requiring fragmentation. This protocol describes a workflow for microbiome characterization and pathogen detection through the extraction, sequencing, and analysis of DNA from bat environmental samples, including guano and urine, using tailored approaches for each sample type. Pre-processing of guano and urine is performed prior to DNA extraction using a spin-column-based method to disrupt sample matrices and lyse microorganisms that may be present. This protocol further describes the preparation of sequencing libraries using either extracted DNA directly or polymerase chain reaction (PCR) products targeting the full-length 16S ribosomal RNA gene for prokaryotic and archaeal taxa and a partial 18S ribosomal RNA gene for eukaryotic taxa. PCR amplification of the full-length 16S ribosomal RNA gene is achieved using universal primers. Magnetic bead-based DNA purification is employed at multiple stages, including following PCR amplification of the 16S or 18S ribosomal RNA genes. Library preparation includes end repair, barcode ligation, pooling of barcoded DNA, and adapter ligation. Libraries are sequenced on a nanopore sequencing platform and analyzed using the EPI2ME bioinformatics workflow. Sequencing runs are performed on MinION flow cells, with run settings adjusted using the MinKNOW sequencing software. Runs are configured for 72 h with basecalling enabled for the raw data collected, generating the sequence-read files that are sorted according to barcode assignment. This workflow enables high-throughput sequencing of multiple samples in a single run, and the resulting sequence data are used for taxonomic classification of prokaryotic, archaeal, and eukaryotic organisms present in each sample, supporting wildlife microbiome characterization, environmental surveillance, and pathogen detection.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149169719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhabdomyolysis Following Robot-Assisted Radical Prostatectomy.","authors":"Ibrahim Al-Taie, Florian M Wagenlehner, Mahmoud Farzat","doi":"10.3791/71769","DOIUrl":"https://doi.org/10.3791/71769","url":null,"abstract":"<p><p>Robot-assisted radical prostatectomy remains a favorable treatment option for locally advanced prostate cancer in patients with obstructive lower urinary tract symptoms. However, the steep Trendelenburg lithotomy position required for this procedure can result in serious complications, particularly in patients with obesity. Here, the case of a 66-year-old man with obesity (BMI, 35 kg/m²), insulin-dependent diabetes mellitus, and multiple comorbidities is reported, who underwent robot-assisted radical prostatectomy (console time, approximately 4 h) for clinically staged cT3a cN1 prostate cancer. Immediately after surgery, the patient developed severe gluteal pain, sensorimotor deficits, and massive rhabdomyolysis, resulting in anuric acute kidney injury and respiratory insufficiency. The clinical course was further complicated by heparin-induced thrombocytopenia type II and a grade IV gluteal pressure ulcer. A 22-day intensive care stay was required, including continuous hemodiafiltration and transfusion of 12 units of erythrocytes. With multidisciplinary management, renal function partially recovered, and the patient was discharged with minimal residual neuropathic deficits. This case highlights the importance of comprehensive preoperative risk assessment and provides clinically relevant insights into the recognition and multidisciplinary management of severe complications associated with prolonged positioning during robot-assisted radical prostatectomy.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149167700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental Microglial Secretome Screening in Rat Organotypic Brain Slice Cultures.","authors":"Johannes Falter, Annette Lohmeier, Eva-Maria Stoerr, Janne Koskimäki, Nils Ole Schmidt, Martin Proescholdt","doi":"10.3791/71455","DOIUrl":"https://doi.org/10.3791/71455","url":null,"abstract":"<p><p>Glioblastoma (GBM) is the most common primary malignant brain tumor in adults and is characterized by extensive interactions between tumor cells and glioblastoma-associated microglia and macrophages (GAMM). In the peritumoral region, where residual glioma cells contribute to recurrence, resident microglia represent the predominant immune population within the GAMM and, in general, may shape early tumor-supportive responses. The objective of this study was to establish an ex vivo model to investigate microglia-specific responses to glioma cells in intact brain tissue. Three-dimensional organotypic brain slice cultures (OBSCs) retaining endogenous microglia were generated and compared with microglia-depleted slices produced by pharmacological CSF1R inhibition with PLX5622. OBSCs were co-cultured for seven days with fluorescently labeled rat glioma spheroids or non-tumorous astrocyte spheroids under defined treatment conditions. Conditioned media were collected for multiplex chemokine analysis, while fluorescence microscopy was used to quantify spheroid invasion and outgrowth. Following culture, OBSCs were cryosectioned and immunostained to characterize cellular and molecular changes in the peri-spheroidal tissue. The model preserved the three-dimensional brain parenchymal environment and enabled comparison of glioma-associated responses in the presence and absence of resident microglia without contributions from peripheral macrophages. Microglial depletion enabled the identification of microglia-dependent changes in chemokine profiles and spheroid behavior during the defined culture period. This experimentally tractable platform models early glioma-microglia interactions and supports mechanistic studies and evaluation of microglia-directed therapeutic strategies in a physiologically relevant tissue context.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149170397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-Frequency Repetitive Transcranial Magnetic Stimulation with Sensory Arousal Nursing for Post-Stroke Dysphagia.","authors":"Chunling Hu, Ling Liu, Shanhua Mao, Meihong Ming, Min Chen, Zhuang Yue","doi":"10.3791/72231","DOIUrl":"10.3791/72231","url":null,"abstract":"<p><p>Post-stroke dysphagia can compromise airway protection, nutrition, and functional recovery. This article describes a clinical protocol combining low-frequency repetitive transcranial magnetic stimulation (rTMS) with a structured sensory arousal nursing package for patients with acute ischemic stroke and dysphagia accompanied by feeding-related upper-limb dysfunction. Dysphagia is the primary treatment focus; upper-limb activities address associated limitations in self-feeding. The protocol integrates screening, stimulation safety review, motor-threshold determination, contralesional swallowing-motor targeting, 1 Hz rTMS, sequenced sensory input, feeding-related task practice, and outcome assessment. Representative results come from a single-center, randomized, assessor-blinded study of 160 patients (80 per group) comparing the combined intervention with routine nursing for swallowing rehabilitation over 2 weeks. The between-group difference in Standardized Swallowing Assessment change was -3.9 points (95% confidence interval [CI], -5.8 to -2.0; p < 0.001). The between-group change-score differences were 0.8 levels for the Functional Oral Intake Scale (95% CI, 0.4 to 1.2; p < 0.001) and -0.9 points for the Penetration-Aspiration Scale (95% CI, -1.5 to -0.3; p = 0.004), the latter restricted to participants with paired instrumental assessments (48 versus 46). Aspiration pneumonia occurred in 5/80 versus 11/80 participants (p = 0.118); no serious rTMS-related adverse events were reported. These findings indicate short-term differences in scale scores but do not establish a clinically important benefit in swallowing or a reduced risk of pneumonia. The protocol coordinates established rehabilitation components. Further studies are needed to determine their individual effects and identify patients who may benefit from contralesional inhibition.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149168993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gua Sha (Scraping Therapy) for Spasticity After Stroke: Systematic Review and Meta-Analysis of RCTs with Integrated Preclinical Mechanistic Synthesis.","authors":"Fei Li, Jun-Xiang Wang, Jin-Shan Zhong, Shi-Jing-Xin Tian, Xiang Li","doi":"10.3791/71890","DOIUrl":"10.3791/71890","url":null,"abstract":"<p><p>​Emerging clinical data from Chinese randomized controlled trials (RCTs) suggest Gua Sha (scraping therapy) may deliver adjunct antispastic benefits for spasticity after stroke (SAS) when combined with standard rehabilitation. This study aimed to synthesize clinical efficacy evidence and explore treatment parameters for this intervention. We searched eight electronic databases from database inception to January 1, 2026; two independent reviewers completed study screening, risk-of-bias assessment using Cochrane Risk of Bias 2.0 tool, and data extraction. Meta-analytic effect sizes were calculated using mean difference (MD), and the review protocol was preregistered on PROSPERO. Sixteen eligible RCTs enrolling 1227 stroke survivors were included, all conducted in China, which may limit the external transferability of our results. Pooled random-effects estimates indicated that add-on scraping therapy was associated with reduced Modified Ashworth Scale and Visual Analog Scale for Pain (VAS-P) scores, lower serum hs-CRP and Hcy, as well as improved motor function and activities of daily living. Grading of Recommendations Assessment (GRADE) rated the certainty of evidence for all outcomes as very low due to high heterogeneity, methodological limitations, suspected publication bias and limited cross-regional data. Exploratory subgroup analyses identified numerically larger spasticity relief in regimens with 5-day treatment intervals, 1.5-month treatment duration and intensive scraping stimulation. These cross-study comparisons generate preliminary hypotheses awaiting verification through dedicated head-to-head dosing trials. Proposed mechanistic pathways linking scraping therapy to symptom relief rely largely on preclinical evidence, including enhanced local microcirculation and modulation of inflammatory and neural signaling. Current evidence supports scraping therapy as an investigational adjunctive strategy for SAS. Given the low-certainty evidence base and lack of inclusion in international rehabilitation guidelines, this therapy should be evaluated only in clinical settings with certified practitioners and standardized adverse event monitoring. Further well-designed RCTs are required to clarify its long-term therapeutic benefits and complete safety profile.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149171135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}