Jove-Journal of Visualized Experiments最新文献

Erratum: Intravital Microscopy of the Inguinal Lymph Node. 勘误：腹股沟淋巴结的活体显微镜检查。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-30 DOI: 10.3791/6652

Stephanie L Sellers, Geoffrey W Payne

引用次数: 0

Facial Vein Venipuncture for Murine Blood Collection. 面部静脉穿刺用于小鼠采血。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-26 DOI: 10.3791/68776

Stephanie Kalinowski, Claudia V Jakubzick

引用次数: 0

High-definition Transcranial Direct Current Stimulation over Right Dorsolateral Prefrontal Cortex to Enhance Metacognitive Sensitivity. 高清晰度经颅直流电刺激右背外侧前额叶皮层增强元认知敏感性。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-26 DOI: 10.3791/68824

Xiangyi Lyu, Jun Wu, Shinan Zhao, Tao Liu, Helen Hong Yang, Zhigang Ma, Jialu Qin

{"title":"High-definition Transcranial Direct Current Stimulation over Right Dorsolateral Prefrontal Cortex to Enhance Metacognitive Sensitivity.","authors":"Xiangyi Lyu, Jun Wu, Shinan Zhao, Tao Liu, Helen Hong Yang, Zhigang Ma, Jialu Qin","doi":"10.3791/68824","DOIUrl":"https://doi.org/10.3791/68824","url":null,"abstract":"In human-AI collaboration, task delegation is a critical component. Ideally, if a person believes they are capable of completing a task, they should do so themselves; otherwise, the task should be delegated to the other party. Such delegation decisions are influenced by individuals' assessments of their own abilities, which are shaped by metacognition-the capacity to evaluate one's own capacities. High-Definition Transcranial Direct Current Stimulation (HD-tDCS) is a non-invasive brain stimulation technology that can regulate the excitability of the cerebral cortex to improve human performance. This protocol presents a method that employs HD-tDCS to investigate its effects on human metacognition during human-AI delegation tasks, detailing the procedure for applying anodal, cathodal, or sham stimulation to the dorsolateral prefrontal cortex (DLPFC). Stimulation was performed using a portable HD-tDCS device, using a circumferential arrangement of five electrodes, including one target electrode and four return electrodes. The stimulation lasted for a total of 21 min, and the current intensity was 2 mA. This method is expected to provide a new research path for further in-depth exploration of the neural mechanisms and behavioral regulation of humans in the process of human-AI collaborative task delegation and provide a solid theoretical basis for further optimizing human-AI collaborative tasks.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Separation and Fractionation of Cell Wall and Cell Membrane Proteins from Mycobacterium tuberculosis for Downstream Protein Analysis. 结核分枝杆菌细胞壁和细胞膜蛋白的分离与分离及其下游蛋白分析。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-26 DOI: 10.3791/67680

Anbarasu Deenadayalan, Madhavan Dhanapal, Uma Devi Ranganathan, Ramalingam Bethunaickan

{"title":"Separation and Fractionation of Cell Wall and Cell Membrane Proteins from Mycobacterium tuberculosis for Downstream Protein Analysis.","authors":"Anbarasu Deenadayalan, Madhavan Dhanapal, Uma Devi Ranganathan, Ramalingam Bethunaickan","doi":"10.3791/67680","DOIUrl":"https://doi.org/10.3791/67680","url":null,"abstract":"Mycobacterium cell wall and membrane proteins, which play a central role in tuberculosis pathogenesis, were successfully separated using preparative Isoelectric Focusing (IEF) and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Followed by gel elution, overcoming limitations in conventional methods for the separation of hydrophobic proteins. In this procedure, M. tuberculosis colonies were transferred from Lowenstein-Jensen slants into 2 mL of 7H9 broth, dispersed with glass beads, and incubated at 37 °C for 2 weeks. Then, the culture was scaled up to 200 mL and grown in a shaker for 4 weeks. It was further upscaled to 1 L with 500 mL of 7H9 broth and grown for an additional 4 weeks. Grown Mycobacteria were pelleted by centrifugation at 1741 × g for 30 min. For each 2 g pellet, 1 mL of breaking buffer was added, and the sample was sonicated. The lysate was centrifuged at 3436 × g for 15 min to remove unbroken cells, and the supernatant was concentrated. This supernatant (whole cell lysate) was centrifuged at 13751 × g for 30 min to pellet cell wall proteins. The remaining supernatant was ultra-centrifuged at 100,000 × g for 4 h to separate the cell membrane and cytosol. The isolated cell wall and membrane proteins were loaded onto a liquid preparative IEF system at 4 °C and separated at 12 W until the voltage stabilized at 1400 V, which separates 20 fractions. These IEF fractions were further separated by preparative SDS-PAGE, and proteins were eluted using a whole gel eluter at 250 mA, resulting in 30 fractions. Through this protocol, we were able to identify novel M. tuberculosis cell walls and membrane-specific biomarkers, and it also shows potential for characterizing similar proteins in other pathogens.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacophore Modeling for Targets with Extensive Ligand Libraries: A Case Study on SARS-CoV-2 Mpro. 具有广泛配体文库的靶标药效团建模——以SARS-CoV-2 Mpro为例

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-26 DOI: 10.3791/68933

L J Córdova-Bahena, S M Pérez-Tapia, Marco A Velasco-Velázquez

{"title":"Pharmacophore Modeling for Targets with Extensive Ligand Libraries: A Case Study on SARS-CoV-2 Mpro.","authors":"L J Córdova-Bahena, S M Pérez-Tapia, Marco A Velasco-Velázquez","doi":"10.3791/68933","DOIUrl":"https://doi.org/10.3791/68933","url":null,"abstract":"A pharmacophore defines the spatial arrangement of molecular features required for optimal interactions between a compound and its biological target. These models can be derived by analyzing the intermolecular interactions between a target and a set of known ligands in their binding conformations. A consensus pharmacophore integrates common features from multiple ligands, reducing model bias and enhancing predictive power. However, generating a robust consensus pharmacophore from a large and chemically diverse ligand set presents technical challenges. Here, we present a protocol for the construction of consensus pharmacophores using ConPhar, an open-source informatics tool designed to identify and cluster pharmacophoric features across multiple ligand-bound complexes. The protocol includes model generation, refinement, and application to the virtual screening of ultra-large molecular libraries. As a case study, we applied the method to the SARS-CoV-2 main protease (Mpro), using one hundred non-covalent inhibitors co-crystallized with the target. The resulting pharmacophore model captured key interaction features in the catalytic region of Mpro and enabled the identification of new potential ligands. This strategy is broadly applicable to any biological target for which ligand-bound conformations are available. It is particularly valuable for targets with extensive ligand datasets and supports rational drug discovery by streamlining the identification of novel candidates with desired interaction profiles.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Cross-Sectional Study of the Association of Serum Uric Acid Levels and Sarcopenia using the China Health and Retirement Longitudinal Study (CHARLS). 使用中国健康与退休纵向研究（CHARLS）对血清尿酸水平与肌肉减少症相关性的横断面研究

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-26 DOI: 10.3791/68961

Kang Luo, Wenjing Xia, Yingxiao Zhang, Jing Yu, Min Zhou, Pan Cheng, Yongxin Wu, Lingjie Xu

{"title":"A Cross-Sectional Study of the Association of Serum Uric Acid Levels and Sarcopenia using the China Health and Retirement Longitudinal Study (CHARLS).","authors":"Kang Luo, Wenjing Xia, Yingxiao Zhang, Jing Yu, Min Zhou, Pan Cheng, Yongxin Wu, Lingjie Xu","doi":"10.3791/68961","DOIUrl":"https://doi.org/10.3791/68961","url":null,"abstract":"Previous research has yielded inconsistent findings concerning the relationship between serum uric acid (UA) levels and sarcopenia. However, there is currently no research that comprehensively examines this relationship within the broader Chinese population. This study aims to explore the relationship between serum uric acid levels and sarcopenia in Chinese adults aged 45 and above, focusing specifically on age-related variations. The present study involved 10,938 participants of the 2015 China Health and Retirement Longitudinal Study (CHARLS). The associations between sarcopenia (including its components) and serum uric acid levels were evaluated using weighted logistic and weighted linear regression models. After categorizing participants by age groups, the subgroup analysis conducted allowed for a more detailed examination of age-related changes. Participants were stratified into quartiles based on their uric acid levels. Adjusted analyses revealed that a higher serum uric acid level was negatively associated with sarcopenia only in individuals aged 65 and older. Results from weighted linear regression analysis indicated a statistically significant positive correlation between serum uric acid levels and both handgrip strength (HGS) and skeletal muscle index (SMI). Moreover, the Q4 group (≥5.70 mg/dL) sustained this positive correlation across all ages. The results showed that higher UA levels were significantly associated with increased SMI and HGS in Chinese people aged 45 years and older. Elevated levels of blood uric acid may potentially exert a safeguarding influence against the onset of sarcopenia, particularly in individuals aged 65 years and above.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous 3D Analysis of Cardiac Damage and Immune Response in Reperfused Acute Myocardial Infarction Using Light Sheet Fluorescence Microscopy. 用荧光显微镜同时三维分析再灌注急性心肌梗死的心脏损伤和免疫反应。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-26 DOI: 10.3791/68347

Sebastian Korste, Elias Haj-Yehia, Simon F Merz, Lea Bornemann, Pia Stock, Matthias Gunzer, Ulrike B Hendgen-Cotta, Tienush Rassaf, Matthias Totzeck

{"title":"Simultaneous 3D Analysis of Cardiac Damage and Immune Response in Reperfused Acute Myocardial Infarction Using Light Sheet Fluorescence Microscopy.","authors":"Sebastian Korste, Elias Haj-Yehia, Simon F Merz, Lea Bornemann, Pia Stock, Matthias Gunzer, Ulrike B Hendgen-Cotta, Tienush Rassaf, Matthias Totzeck","doi":"10.3791/68347","DOIUrl":"https://doi.org/10.3791/68347","url":null,"abstract":"Complex cellular interactions determine functional and structural tissue remodeling during reperfused acute myocardial infarction (repAMI). These processes show distinct spatial distribution as the injured heart muscle is segmented into different areas (damage area, area at risk (AAR), and remote area). Three-dimensional (3D) visualization of these areas is essential for the analysis of various interactions between resident cardiac cells and infiltrating immune cells, enabling the identification of possible treatment targets. Here, a protocol is described for simultaneous and automatable 3D visualization and quantification of the cardiac damage area, AAR, and infiltrating immune cells (e.g., neutrophils) after repAMI. This includes intravital antibody-mediated staining of cardiac damage area (CD31neg) and neutrophil infiltration (Ly6G+) following ex vivo visualization of AAR by retrograde antibody perfusion and further non-toxic tissue clearing for light sheet fluorescence microscopy (LSFM) imaging. This technique allows the spatial analysis of target cells, e.g., infiltrating immune cells and damaged areas in an intact mouse heart after repAMI. Traditional histology and immunohistochemistry can be performed after non-toxic tissue clearing and image acquisition with computer-assisted post-processing. This allows multiplexing information gain within the same mouse heart, strengthening data robustness and being especially significant in a highly complex injury like repAMI.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smart Nursing-Based Tripartite Dual-Track Interactive Nursing in Elderly Patients with Coronary Heart Disease Complicated by the "Three Highs". 基于智能护理的老年冠心病“三高”患者三方双轨互动护理

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-26 DOI: 10.3791/68948

Wei Ji, Qixia Zhu

{"title":"Smart Nursing-Based Tripartite Dual-Track Interactive Nursing in Elderly Patients with Coronary Heart Disease Complicated by the \"Three Highs\".","authors":"Wei Ji, Qixia Zhu","doi":"10.3791/68948","DOIUrl":"https://doi.org/10.3791/68948","url":null,"abstract":"The objective of this study was to evaluate the effectiveness of the Tripartite Dual-Track Interactive Nursing (TDTIN) model, incorporating smart nursing technologies, in elderly patients with coronary heart disease (CHD) complicated by hypertension, hyperglycemia, and hyperlipidemia (collectively termed the \"three highs\"), and to verify its improvement effects on patients' self-care capacity, quality of life, and psychological well-being. A randomized controlled trial was conducted with 162 elderly CHD patients with the \"three highs,\" allocated equally into an observation group (n = 81, receiving smart nursing-based TDTIN) and a conventional group (n = 81, receiving conventional care). Both groups were monitored for 3 months post-discharge. Standardized assessments included the Exercise of Self-Care Agency (ESCA) scale for self-care ability, the Barthel Index (BI) for activities of daily living, the Hamilton Anxiety (HAMA) and Depression (HAMD) scales for psychological status, the Morisky Medication Adherence Scale for compliance, and a satisfaction survey. Compared to the conventional group, the observation group demonstrated significantly greater improvements in self-care ability (ESCA) and quality of life (BI), along with lower HAMA and HAMD scores (P < 0.05). The observation group also exhibited higher treatment compliance (Morisky) and satisfaction ratings (P < 0.05). Additionally, caregivers in the observation group showed enhanced caregiving skills and support scores (P < 0.05). The TDTIN model, leveraging smart nursing, significantly enhances self-care capacity, quality of life, treatment compliance, and psychological outcomes in elderly CHD patients with the \"three highs,\" while strengthening family-community support systems.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2. 脂质体膜通透性试验研究磷脂酰肌醇磷酸基团对蛇毒PLA2嗜膜作用的影响。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-26 DOI: 10.3791/68594

Zhuoyan Zeng, Mingsi Wei, Kaixin Zheng, Zimu Zhou, Shuhao Zhang, Yanglin Guo, Yuri N Utkin, Ruben K Dagda, Edward S Gasanoff

{"title":"A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2.","authors":"Zhuoyan Zeng, Mingsi Wei, Kaixin Zheng, Zimu Zhou, Shuhao Zhang, Yanglin Guo, Yuri N Utkin, Ruben K Dagda, Edward S Gasanoff","doi":"10.3791/68594","DOIUrl":"https://doi.org/10.3791/68594","url":null,"abstract":"We developed a novel, cost-effective, and highly sensitive assay for assessing liposomal membrane permeability based on ligand substitution reactions involving ligands encapsulated in the inner volume of liposomes, which we combined with a hydrolytic activity assay (using coenzyme A acylation) and molecular modeling simulation to investigate whether phosphates of the inositol ring in phosphatidylinositol protect membranes from the damaging effects of aberrantly expressed enzymes. Aberrant expression of phospholipases A2 (PLA2) leads to demyelination of axons that triggers the development of multiple sclerosis and Alzheimer's disease. To mimic the effects of aberrantly expressed PLA2, we used the basic subunit of viper venom PLA2, HDP-2P. The HDP-2P was tested on phosphatidylcholine (PC) liposomes enriched with phosphatidylinositol, phosphatidylinositol-4-phosphate (PI-4-P), or phosphatidylinositol-4,5-diphosphate (PI-4,5-P2). While HDP-2P showed minimal hydrolytic activity and only slightly increased permeability in PC liposomes, enrichment of PC liposomes with PI enhanced both hydrolysis and permeability. Remarkably, PI-4-P and PI-4,5-P2 dramatically inhibited HDP-2P's hydrolytic activity and completely reduced permeability. Molecular modeling simulations revealed that phosphates of the inositol ring in phosphatidylinositol sterically block phospholipid access to HDP-2P's active site, preventing hydrolysis. Both in vitro and in silico data suggest that phosphorylated phosphatidylinositols are associated with reduced PLA2 activity, indicating a potential mechanism for mitigating excessive PLA2 activity -- presumably as a means of protecting cell membranes from degeneration.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-22-Mediated Regulation of Wnt/β-Catenin Signaling by Curcumin in Retinoblastoma. mir -22介导的姜黄素在视网膜母细胞瘤中对Wnt/β-Catenin信号的调控。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-26 DOI: 10.3791/69300

Peng Li, Li Zheng, Qing Yang, Wanrong Huang, Xinlin Yan

{"title":"miR-22-Mediated Regulation of Wnt/β-Catenin Signaling by Curcumin in Retinoblastoma.","authors":"Peng Li, Li Zheng, Qing Yang, Wanrong Huang, Xinlin Yan","doi":"10.3791/69300","DOIUrl":"https://doi.org/10.3791/69300","url":null,"abstract":"Retinoblastoma (RB) is a common intraocular malignant tumor affecting infants and children, yet its precise etiology and pathogenesis remain incompletely understood. Curcumin, a bioactive polyphenol, inhibits tumor progression via microRNA-mediated modulation of the Wnt/β-catenin signaling cascade. This study aimed to clarify how curcumin mediates its antitumor effects in RB by investigating its regulation of miRNA-22 (miR-22) expression and exploring the underlying molecular mechanisms. Two validated retinoblastoma models (SO-RB50/WERI-Rb-1) were treated with curcumin at varying concentrations. To delineate miR-22's regulation of Wnt/β-catenin signaling, target cells were transduced with either a miR-22 mimic lentivirus or a non-functional control lentivirus. Xenograft tumor models were established in mice using human RB cells to observe the in vivo effects of curcumin on tumor size, miR-22 expression, and Wnt/β-catenin protein levels. Cellular proliferation, invasion, and apoptosis were assessed using the CCK-8, Transwell, and Annexin V-APC-PI dual staining assay, respectively. miR-22 levels were quantified by RT-PCR, and Wnt1 and β-catenin expression profiles were determined by Western blot analysis. Curcumin treatment resulted in decreased proliferation and invasiveness in RB cells, while enhancing apoptosis and elevating miR-22 expression. Inhibition of miR-22 diminished curcumin's effects on the Wnt/β-catenin signaling pathway. In xenograft studies, curcumin significantly reduced tumor size and enhanced miR-22 expression within the tumors, effectively suppressing Wnt/β-catenin signaling. These findings demonstrate that curcumin inhibits RB cell proliferation and invasiveness while promoting apoptosis, primarily mediated through miR-22 upregulation and subsequent inhibition of the Wnt/β-catenin pathway.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0