Jove-Journal of Visualized Experiments最新文献

{"title":"Erratum: Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging.","authors":"Alessandra Nardini, Behjat Sadat Kariman, Mario Marini, Conci Conci, Marco Grassi, Margaux Bouzin, Maddalena Collini, Roberto Osellame, Claudio Cerullo, Manuela Teresa Raimondi, Giuseppe Chirico, Rebeca Martínez Vázquez","doi":"10.3791/6637","DOIUrl":"https://doi.org/10.3791/6637","url":null,"abstract":"<p><p>This corrects the article 10.3791/67975.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 220","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144227204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum: Modified Mouse Model of Repetitive Mild Traumatic Brain Injury Incorporating Thinned-Skull Window and Fluid Percussion.","authors":"Yuncheng Liu, Haojie Mao, Shiqiang Chen, Jihui Wang, Wei Ouyang","doi":"10.3791/6636","DOIUrl":"https://doi.org/10.3791/6636","url":null,"abstract":"<p><p>This corrects the article 10.3791/66440.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 220","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144227205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial Preparation from Microglia for Glycan Analysis.","authors":"Meghana Madabhushi, Tana V Palomino, Mallikarjun H Patil, David C Muddiman, Daniel J Tyrrell, Juhi Samal","doi":"10.3791/68179","DOIUrl":"https://doi.org/10.3791/68179","url":null,"abstract":"<p><p>Understanding the glycosylation patterns of mitochondrial proteins in microglia is critical for determining their role in neurodegenerative diseases. Here, we present a novel and high-throughput methodology for glycomic analysis of mitochondrial proteins isolated from cultured microglia. This method involves the isolation of mitochondria from microglial cultures, quality assessment of mitochondrial samples, followed by an optimized protein extraction to maximize glycan detection, and infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) high-resolution accurate mass (HRAM) mass spectrometry to provide detailed profiles of mitochondrial glycosylation. This protocol emphasizes the importance of maintaining mitochondrial integrity during isolation and employs stringent quality control to ensure reproducibility, including measuring mitochondrial purity after extraction. This approach allows for the comprehensive profiling of glycosylation changes in microglial mitochondria under various experimental conditions in vitro, which offers insight into mitochondrial changes associated with neurodegenerative diseases. This approach could be adapted to other in vitro treatments, other cultured cell types, or primary cells. Through this standardized approach, we aim to advance the understanding of microglial mitochondrial glycans, contributing to the broader field of neurodegenerative research.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 219","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment Model for Young Patients with Psychogenic Erectile Dysfunction and Resultant Infertility.","authors":"Jieling He, Weifeng Liu, Weilan Huang, Xin Luo, Ge Zou, Pei Du, Ruilin Yang","doi":"10.3791/67132","DOIUrl":"https://doi.org/10.3791/67132","url":null,"abstract":"<p><p>Conventional management of erectile dysfunction (ED)-related non-consummation and infertility often overlooks psychosocial determinants and dyadic involvement, leading to suboptimal therapeutic outcomes, including persistent sexual dysfunction and reduced couple satisfaction. This study proposes and validates a novel, patient-centric intervention tailored for young individuals with psychogenic ED (pED), targeting both erectile recovery and infertility resolution. The \"Streamlined Clinical Process\" incorporates structured treatment strategies for both the patient and their partner, including evidence-based sexual health education, psychological support, partner-inclusive counseling, sensate focus training, lifestyle modification, on-demand pharmacotherapy, and structured follow-up. Following comprehensive baseline assessments (medical history, physical examination, and diagnostics), the treatment team collaboratively formulates individualized 16-week regimens with active partner engagement. Three key innovations distinguish this protocol: (1) The implementation of the GLTC (Goodwill-Listening-Talking-Cooperation) communication framework, which addresses patients' psychological needs and promotes effective communication strategies; (2) The application of Hawton's psychosexual stratification to identify specific psychological factors, with the aim of reshaping negative sexual beliefs, alleviating anxiety, improving sexual function, and rebuilding mutual trust and emotional connection between partners; (3) The development of easy-to-understand videos for psychological education to enhance treatment adherence through iterative patient-provider dialogue. Results demonstrate clinically meaningful improvements in erectile function recovery rates, coital success frequency, and couple satisfaction. Notable challenges include patient adherence to the treatment plan and the timely effectiveness of non-pharmacological interventions. In conclusion, this model presents a scalable and effective strategy for addressing sexual dysfunction-related infertility in young patients, offering a valuable framework for clinical practice and future research in sexual medicine.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 219","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determining Four Components in a Lipid Nanoparticle RNA Delivery System by Liquid Chromatography Combined with Evaporative Light Scattering Detector.","authors":"Jia Zheng, Huijuan Jiang, Junqi Huang, Qiaoxia Liu, Hongyuan Hao","doi":"10.3791/67711","DOIUrl":"https://doi.org/10.3791/67711","url":null,"abstract":"<p><p>This article presents a method for the analysis of lipid nanoparticle (LNP) components. LNP, serving as a pivotal vector for RNA-based drugs, primarily consists of cholesterol, PEG with modifications, ionizable lipids, and helper lipids. These components exhibit weak polarity, leading to strong retention and difficulty in separation using reverse-phase chromatography, as well as lacking distinct ultraviolet absorption characteristics. In order to address this challenge, a liquid chromatography system was coupled with an evaporative light-scattering detector (ELSD). By systematically adjusting the type of chromatographic column and optimizing the gradient elution program of the mobile phase, rapid and complete baseline separation of the four critical components was accomplished. Utilizing the latest ELSD technology enhanced detection sensitivity significantly and expanded the linear range of the method. Experimental results demonstrated that within a concentration range of 5 µg/mL to 250 µg/mL, the four components of LNP displayed excellent linearity with correlation coefficients all greater than 0.999, and the accuracy ranged from 94.2% to 108.0%. In precision experiments, the relative standard deviations of both retention time and peak area for a 10 µg/mL standard solution were below 0.1% and 2%, respectively. When using this method to analyze different LNP samples, all components are successfully separated, and their respective contents are accurately quantified, highlighting the robust adaptability of this analytical approach.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 219","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multidisciplinary Approach to Obesity Management: A Case Report.","authors":"Yuval Pinto, Zoobia Wazir Chaudhry, Selvi Rajagopal, Marci Laudenslager, Lawrence J Cheskin","doi":"10.3791/68221","DOIUrl":"https://doi.org/10.3791/68221","url":null,"abstract":"<p><p>Effective weight management care emphasizes a patient-centered approach that employs evidence-based tools and strategies to address the complex and multifactorial nature of obesity and prevent chronic obesity-related medical conditions. Successful outcomes hinge on personalized care, consistent engagement, and the integration of multiple interventions that are tailored to individual needs and circumstances. In the John Hopkins Healthful Eating, Activity and Weight Program (HEAWP), healthcare professionals specializing in obesity medicine work in partnership with other specialists, including behavioral therapists, dieticians, psychologists, psychiatrists, sleep specialists, bariatric surgeons, gastroenterologists, and physical therapists, while also involving patients in their own care. Each patient starts with an in-person intake meeting with an obesity physician, during which information about their nutrition, sleep, mood, medications, social support, and physical exercise is evaluated. By the end of the visit, realistic goals are set in concert with the patient, and the patient is given specific tasks to assist them in accomplishing these goals. The initial phase of the intensive evaluation and treatment at HEAWP typically lasts 6 months, with follow-up medical visits every month. At that point, goals are reassessed and future treatment plans formulated. The average pace of weight loss is 1-2 pounds (0.4-0.9 kg) per week.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 219","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Bladder Management Program Combined with a Biofeedback Stimulator in Neurogenic Bladder Rehabilitation.","authors":"Liping Xin, Maomao Li, Xijun Weng","doi":"10.3791/67806","DOIUrl":"https://doi.org/10.3791/67806","url":null,"abstract":"<p><p>Neurogenic Bladder (NB) refers to the dysfunction of the urinary tract caused by damage to the central nervous system or peripheral nerves that control urination. The combination of Comprehensive Bladder Management (CBM) and biofeedback stimulator is one of the rehabilitation treatments to improve the quality of life and increase the quality of urination for patients with neurogenic bladder. Both aim to enhance the patient's bladder capacity, reduce residual urine volume, and decrease the incidence of urinary incontinence. However, there is no consensus on the therapeutic effect of comprehensive bladder management combined with biofeedback stimulators for patients with neurogenic bladder. This study aims to investigate the safety and effectiveness of bladder comprehensive management combined with a biofeedback stimulator for rehabilitation treatment in patients with neurogenic bladder. In this study, the experimental group underwent a step-by-step implementation of bladder comprehensive management combined with biofeedback stimulator therapy. The treatment was evaluated based on changes in bladder capacity, incidence of urinary incontinence, residual urine volume, and the responses on the International Consultation on Incontinence Questionnaire - Short Form (ICIQ-SF). The results showed significant improvements in all parameters for both groups, with the treatment group achieving superior outcomes. Bladder capacity increased (212.37 ± 45.56 mL to 350.98 ± 93.52 mL), urinary incontinence incidence decreased (46.43% to 7.14%), residual urine volume reduced (149.25 ± 12.25 mL to 49.63 ± 6.96 mL), and ICIQ-SF scores improved (14.12 ± 3.55 to 5.95 ± 2.26). These findings highlight the combined therapy's effectiveness in improving bladder function and reducing incontinence.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 219","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preservation of Porcine Biomodels for the Study of Comparative Human Anatomy.","authors":"Liliana Valladares-Torres, Flavio Edgar Cerón-Betancourth, Jose Guillermo Betancourt-Villalobos, Angelica Benavidez-Jaramillo, Sergio Andrés Montenegro-Herrera","doi":"10.3791/66646","DOIUrl":"https://doi.org/10.3791/66646","url":null,"abstract":"<p><p>The teaching of human anatomy in medical education faces challenges, necessitating effective alternatives for students to practically explore anatomical complexity. Porcine biomodels, with their anatomical similarities to humans, offer a promising solution. This article presents a detailed protocol for preserving porcine biomodels, addressing the need for accessible and efficient methods in comparative anatomy studies. This protocol proposes the use of a modified Chilean solution for biomodel preservation, employing formalinization techniques through repletion and immersion. Subsequently, deformalization is achieved using the modified Chilean solution without formaldehyde, followed by a glycerin softening process. Given the scarcity of literature on preservation techniques and the absence of a standardized procedure or test to evaluate tissue conservation, we suggest assessing tissue quality based on measures of resistance and elasticity. Our findings indicate a qualitatively high level of tissue preservation in our specimens. Furthermore, these biomodels are currently utilized successfully for morphology studies and in teaching human comparative anatomy to medical students.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 219","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Application of Remote-Controlled High-Definition Capsule Endoscopy in Canine Bladder Examination.","authors":"Yang Yuan, Leyi Liu, Dingli Hu, Shihao Zhang, Bing Wang, Yunlong Li","doi":"10.3791/66990","DOIUrl":"https://doi.org/10.3791/66990","url":null,"abstract":"<p><p>This study investigated the feasibility and potential applications of capsule endoscopy for bladder examination using a canine model. Three adult male Beagle dogs underwent surgical implantation of capsule cystoscopes and were divided into three groups, each group with one Beagle: Group A (Beagle A) remained in a fixed supine position for 8 h, Group B (Beagle B) moved freely for 8 h, and Group C (Beagle C) had manually adjusted positions (prone, supine, squatting) for 20 min each. The implanted capsule endoscopes functioned successfully in all dogs. Group A primarily captured images of the bladder base (6 h 27 min). Group B acquired images of the bladder neck and base (7 h 12 min), including dynamic visualization of the bladder neck, prostatic fossa, and external urethral sphincter during natural urination. Group C yielded images of the bladder neck, base, and apex (56 min). The findings of this study demonstrate the ability of capsule endoscopy to provide dynamic, high-quality images of the canine bladder wall and suggest its potential for developing accurate and urodynamic assessments.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 219","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using Optical Tweezers for the Generation of Hybrid Spheroids.","authors":"Kamila Duś-Szachniewicz, Katarzyna Gdesz-Birula, Sławomir Drobczyński","doi":"10.3791/67422","DOIUrl":"https://doi.org/10.3791/67422","url":null,"abstract":"<p><p>Two-dimensional (2D) cell culture is still among the most commonly used models in preclinical cancer research. However, the three-dimensional (3D) in vitro models appear to assess the in vivo environment more accurately, including a more relevant architectural morphology, microenvironment, and responses to drugs. Optical trapping uses one or more focused laser beams to non-invasively manipulate the position, motion, interaction, and dynamics of structures on nano- and microscale. Optical tweezers (OT) have found numerous applications in cell biology; however, their potential in cancer research is still undervalued. In this paper, we recommend a noninvasive protocol for lymphoma-stromal cell spheroid formation with the use of optical tweezers. This method not only constructs the hybrid spheroids de novo but also enables controlling the number of attached cells and the time of adhesion formation, providing an important source of information for tissue engineering. Furthermore, OT enables the study of the early stages of hybrid spheroid formation, which cannot be achieved with standard bulk techniques. Importantly, the described model can be used to study the minimal changes in adhesion induced by anti-cancer drugs. This protocol can be easily applied to other cell types.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 219","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}