Jove-Journal of Visualized Experiments最新文献_第2页

The Circadian Rave System: An Affordable, Open-Source, and Easy-to-Build System for Any Light-Based Experiments. 昼夜节律狂欢系统：一个负担得起的，开源的，易于构建的系统，任何基于光的实验。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-26 DOI: 10.3791/68611

Terrence M Trinca, Oscar A Peña, Amy Preston, Sophie Smith, Callum Nicholls, Derek Carr, Mark A Naven

{"title":"The Circadian Rave System: An Affordable, Open-Source, and Easy-to-Build System for Any Light-Based Experiments.","authors":"Terrence M Trinca, Oscar A Peña, Amy Preston, Sophie Smith, Callum Nicholls, Derek Carr, Mark A Naven","doi":"10.3791/68611","DOIUrl":"https://doi.org/10.3791/68611","url":null,"abstract":"Precise control of lighting has broad applications in all biological sciences, but none more so than in circadian research. Life on Earth has evolved circadian clocks that are endogenous regulators of physiology and behavior that entrain to the 24-h solar day. To study the clock, researchers rely on experimental manipulation of either light or temperature to entrain circadian rhythms. Most laboratories currently use either commercially available or in-house-built lighting equipment to either mimic or disrupt natural day and night cycling. These systems offer limited programmability and precision, lack standardization, and, in some instances, affordability. To address these issues, we have developed the Circadian Rave System, a bespoke software and hardware package that allows the precise control of multiple independent light boxes. We have made the software open source, along with detailed hardware build guides, with the aim that future research can be standardized through the use of this system. To demonstrate the functionality of this system and its utility in circadian experiments, we measured longevity and activity of Drosophila in various light paradigms to show circadian synchronization and disruption through light manipulation. In conclusion, we have developed a standardized, scalable, and affordable lighting system that can be built in-house by any researcher with minimal prior knowledge in electronics or programming.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast. 芽殖酵母细胞周期依赖过程的操作与分析。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-26 DOI: 10.3791/68887

Michael G Stewart, Talia C Scheel, Ahmed A Abouelghar, Sara E Hoppe, Matthew P Miller

{"title":"Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast.","authors":"Michael G Stewart, Talia C Scheel, Ahmed A Abouelghar, Sara E Hoppe, Matthew P Miller","doi":"10.3791/68887","DOIUrl":"https://doi.org/10.3791/68887","url":null,"abstract":"Eukaryotic cells follow a conserved cell cycle that regulates diverse processes, including DNA maintenance and organelle homeostasis. Studying cellular processes in a cell cycle-dependent manner is often necessary to properly interpret experimental results. There are chemical and genetic methods available to produce cell cycle synchronization in cultured cells across a wide swath of organisms, including vertebrate models, enabling the study of cell cycle-dependent processes. However, among model organisms, budding yeast remains a powerhouse for cell cycle analysis due to its particularly robust synchronization methods, short generation time, and genetic tractability. Yeast shares core cell cycle machinery with other eukaryotes, which has enabled landmark discoveries in cell cycle regulation. This protocol details methods for cell cycle analysis in yeast, focusing on G1 arrest-release and mitotic arrest-release experiments, including strain construction, culture preparation, and microscopy. PCR tagging methods for producing suitable strains for cell cycle arrests and fluorescence microscopy are presented. A G1 arrest is achieved using the peptide pheromone α-factor, and brief washes result in synchronous release and cell cycle progression. Samples are taken at different time points following release into the cell cycle and fixed for microscopy. A second method arrests yeast cells in mitosis by depleting the cell cycle regulator Cdc20 to achieve a metaphase-arrested population, as well as optional release into anaphase. Samples are fixed and prepared for imaging pre- and post-release, and are imaged and analyzed. Image analysis focuses on cataloging dynamic localization and population abundance changes of proteins in the cell cycle. These synchronization methods are suitable for diverse cell cycle manipulations, and while their use in imaging fixed cells is highlighted here, they can be adapted for many other analyses, including live cell imaging as well as biochemical and molecular assays.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identifying Immune-related Molecular Biomarkers in Autism Spectrum Disorder Using Data-independent Acquisition Proteomics and Machine Learning. 利用数据独立获取蛋白质组学和机器学习识别自闭症谱系障碍中免疫相关的分子生物标志物。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-26 DOI: 10.3791/68949

Jun He, Qingqing Hu, Sifeng Wang, Xiaohui Gong, Ni Gan, Li Huang, Haofeng Chen, Jie Dai, Hong Yu, Shuanglin Xiang, Xiangwen Peng

{"title":"Identifying Immune-related Molecular Biomarkers in Autism Spectrum Disorder Using Data-independent Acquisition Proteomics and Machine Learning.","authors":"Jun He, Qingqing Hu, Sifeng Wang, Xiaohui Gong, Ni Gan, Li Huang, Haofeng Chen, Jie Dai, Hong Yu, Shuanglin Xiang, Xiangwen Peng","doi":"10.3791/68949","DOIUrl":"https://doi.org/10.3791/68949","url":null,"abstract":"This study presents a reproducible protocol for identifying serum protein biomarkers associated with autism spectrum disorder (ASD) using data-independent acquisition (DIA) mass spectrometry combined with machine learning (ML). DIA enables unbiased, high-resolution profiling of the serum proteome, including low-abundance proteins, while ensuring reproducibility across samples. ML approaches were applied to select diagnostically informative protein panels and improve model robustness. The analysis included serum from 99 children with ASD and 70 age-matched controls. High-abundance proteins were depleted, peptides were prepared using standardized digestion and fractionation procedures, and DIA was performed on a high-resolution mass spectrometer. Data processing and quantification identified differentially expressed proteins, which underwent functional enrichment analysis. Eight immune-related proteins emerged as strong candidates for biomarker development. A logistic regression model trained on these proteins achieved 95.27% accuracy, a Kappa value of 0.9025, and an AUC of 1.000 in cross-validation. These findings demonstrate the potential of DIA-based proteomics, combined with machine learning, as a robust framework for biomarker discovery in ASD and for adaptation in broader clinical research.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development, Characterization, and Evaluation of CAGE-based Ionic Liquid Systems for Transdermal Delivery. 基于笼型离子液体的透皮给药系统的开发、表征和评价。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-26 DOI: 10.3791/68658

Saahil Sandeep Baghel, Maja Munck Nikolajsen, Stefanie Dietl, Ragna G Diedrichsen, Martin Malmsten, Stine Rønholt

{"title":"Development, Characterization, and Evaluation of CAGE-based Ionic Liquid Systems for Transdermal Delivery.","authors":"Saahil Sandeep Baghel, Maja Munck Nikolajsen, Stefanie Dietl, Ragna G Diedrichsen, Martin Malmsten, Stine Rønholt","doi":"10.3791/68658","DOIUrl":"https://doi.org/10.3791/68658","url":null,"abstract":"Ionic liquids have recently gained remarkable recognition for their applications within drug delivery, in particular their potential to enable cutaneous drug penetration and enhance drug permeation. In addition to enhancing barrier permeability, ionic liquids have excellent solvation properties, addressing the challenge of dissolving poorly soluble drugs. Due to the large asymmetry between the organic cations and anions, these functional salts remain liquid at room temperature. From an application perspective, ionic liquids provide a promising and highly tunable approach to serve multiple therapeutic areas. A biocompatible ionic liquid composed of choline and geranic acid (CAGE), in a stoichiometric ion ratio of 1:2, holds immense potential for transdermal delivery of a wide range of active pharmaceutical ingredients, including small molecules, peptides, proteins, and nucleic acids. This has a minimal impact on skin barrier function. Herein, we share our methods for the preparation, characterization, and evaluation of CAGE. Representative results are shown for CAGE characterization using NMR spectroscopy, differential scanning calorimetry, Karl-Fischer titration for water content analysis, and ex vivo barrier interaction studies on porcine skin.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recombineering-based Manufacturing of Engineered Viral Vectors for Research and Therapy. 用于研究和治疗的工程病毒载体的重组制造。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-26 DOI: 10.3791/68988

Annagiulia Scognamiglio, Simone Totaro, Alfredo Cipriano, Alfredo Nicosia, Nicola Zambrano, Emanuele Sasso, Guendalina Froechlich

{"title":"Recombineering-based Manufacturing of Engineered Viral Vectors for Research and Therapy.","authors":"Annagiulia Scognamiglio, Simone Totaro, Alfredo Cipriano, Alfredo Nicosia, Nicola Zambrano, Emanuele Sasso, Guendalina Froechlich","doi":"10.3791/68988","DOIUrl":"https://doi.org/10.3791/68988","url":null,"abstract":"The increasing demand for engineered viral vectors in both basic and translational research has underscored the need for flexible, rapid, and scalable methods to generate recombinant DNA viruses. Strategies relying on restriction enzyme digestion and ligation are constrained by sequence-dependent limitations and time-consuming cloning steps. Here, we describe the recombination-mediated genetic engineering method (recombineering) that circumvents these limitations by enabling precise and seamless modifications of viral genomes in bacterial artificial chromosomes (BACs). This approach allows for efficient deletion, insertion, or substitution of coding or non-coding genetic elements, providing a powerful platform for high-throughput viral vector development. Recombineering is particularly valuable in basic research applications, such as the deletion or mutation of viral genes to investigate their function. More importantly, this methodology enables the generation of tens of recombinant viruses encoding distinct immunostimulatory or therapeutic payloads in parallel, making it exceptionally well-suited for the rapid preclinical evaluation of novel constructs. While this technology can be potentially implemented for any scientific purpose, this article focuses on the application of recombineering in two specific areas: the generation of oncolytic viruses based on herpes simplex virus, and the development of non-replicative adenoviral vectors for gene transfer. In conclusion, recombineering offers a versatile approach to viral genome engineering, significantly accelerating the pipeline from design to functional testing. Its relevance spans from fundamental virology to translational medicine to meet evolving research and clinical needs.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term Culture of Human Primordial Germ Cell-like Cells. 人类原始生殖细胞样细胞的长期培养。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-26 DOI: 10.3791/68679

Keiko Shioda, Mutsumi Kobayashi, Misato Kobayashi, Junko Odajima, Hikari Hagihara, Yoko Hashimoto, Takuto Yamamoto, Toshihiro Shioda

{"title":"Long-term Culture of Human Primordial Germ Cell-like Cells.","authors":"Keiko Shioda, Mutsumi Kobayashi, Misato Kobayashi, Junko Odajima, Hikari Hagihara, Yoko Hashimoto, Takuto Yamamoto, Toshihiro Shioda","doi":"10.3791/68679","DOIUrl":"https://doi.org/10.3791/68679","url":null,"abstract":"Primordial germ cells (PGCs) are common precursors of all male and female germline cells. PGCs arise in peri-implantation mammalian embryos and become gender-specific germ cell precursors upon sexual differentiation of the gonadal anlage. The in vitro model commonly known as PGC-Like Cells (PGCLCs), generated from human pluripotent stem cells, is a useful surrogate of the human embryonic PGCs, providing a unique opportunity to explore human gametogenesis in vitro. The protocol presented here supports long-term in vitro expansion of human PGCLCs. Freshly isolated PGCLCs are maintained initially on a STO feeder layer and then expanded in a feeder-free condition on basement membrane extract. The long-term culture PGCLCs (LTC-PGCLCs) can be Fluorescence-Activated Cell Sorting (FACS)-enriched as CD38-positive cells and are readily amplified to tens of millions of cells as pure, feeder-free, and serum-free monotonous culture without apparent limitations such as senescence. It is practically feasible to obtain a pure population of more than 1 million human PGCLCs from a few thousand freshly isolated PGCLCs. The human LTC-PGCLC cell culture is a useful and convenient in vitro model to study human germ cell biology and differentiation into the downstream germline cells.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gold Futures Price Prediction Using Transformer Deep Learning Models with Data Scraped via UiPath. 基于UiPath抓取数据的Transformer深度学习模型的黄金期货价格预测

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-26 DOI: 10.3791/68903

Sirisha Charugulla, Shaiku Shahida Saheb

{"title":"Gold Futures Price Prediction Using Transformer Deep Learning Models with Data Scraped via UiPath.","authors":"Sirisha Charugulla, Shaiku Shahida Saheb","doi":"10.3791/68903","DOIUrl":"https://doi.org/10.3791/68903","url":null,"abstract":"Gold is one of the most valuable and widely traded commodities worldwide, particularly in India, and plays a significant role in economic and financial markets. Historically, gold has been a cornerstone of international trade and economic stability, with central banks maintaining reserves to manage inflation and foreign debt. The price of gold serves as a key economic indicator that influences market trends and investment strategies. However, accurately predicting gold prices is challenging due to the complex and nonlinear nature of financial markets which are influenced by various factors including interest rates, economic recessions, oil price fluctuations, and geopolitical events. The study transformer model was used to predict the daily gold prices which were collected from investing.com through web scraping by using UiPath. It is a Robotic Process Automation (RPA) platform to preserve the integrity of the data and enhance model performance, preprocessing operations such as missing data handling and MinMax scaling were performed. The model was tested and trained on key performance metrics and achieved a Mean Squared Error (MSE) of 0.0224, Root Mean Squared Error (RMSE) of 0.1496, and R-squared of 0.93, with a high prediction accuracy. The study results confirm that the transformer model efficiently detects short-term price movements and long-term market trends offering a more accurate and dependable method than traditional forecasting methods. The study provides valuable guidance to investors, financial analysts and policymakers in making informed decisions in the gold bullion market. Future research can be improved by the inclusion of alternative data sources such as sentiment from news headlines and social media which can potentially offer richer insight into market movements.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rare Pathogen in Diabetic Foot Gangrene: A Case of Wohlfahrtiimonas chitiniclastica Infection. 罕见致病菌致糖尿病足坏疽：1例几丁质粒化沃尔法蒂单胞菌感染。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-23 DOI: 10.3791/68877

Weijie Zhou, Dan Zhang, Ahmad Alhaskawi, Sohaib Hasan Abdullah Ezzi, Vishnu Goutham Kota, Mohamed Hasan Abdulla Hasan Abdulla, Ali Hasan Abdulla Hasan Abdulla, Sahar Ahmed Abdalbary, Hui Lu, Jinhui Liang

{"title":"Rare Pathogen in Diabetic Foot Gangrene: A Case of Wohlfahrtiimonas chitiniclastica Infection.","authors":"Weijie Zhou, Dan Zhang, Ahmad Alhaskawi, Sohaib Hasan Abdullah Ezzi, Vishnu Goutham Kota, Mohamed Hasan Abdulla Hasan Abdulla, Ali Hasan Abdulla Hasan Abdulla, Sahar Ahmed Abdalbary, Hui Lu, Jinhui Liang","doi":"10.3791/68877","DOIUrl":"https://doi.org/10.3791/68877","url":null,"abstract":"Wohlfahrtiimonas chitiniclastica is a rare Gram-negative bacterium typically associated with wound infections, particularly in immunocompromised patients or individuals exposed to unsanitary conditions. Although clinical cases are infrequent, the infection can lead to severe complications such as bacteremia, septic shock, and even death if unrecognized or inadequately treated. We present the case of a 76-year-old diabetic male who developed chronic, non-healing foot ulcers complicated by gangrene and maggot infestation. Diagnostic challenges were addressed using metagenomic next-generation sequencing, which identified W. chitiniclastica alongside Proteus mirabilis and Corynebacterium striatum. Management included aggressive surgical debridement to remove necrotic tissue, local application of gentamicin-impregnated bone cement, systemic antibiotic therapy with ertapenem, and wound reconstruction using a dorsally based fascial flap. This combined approach resulted in significant clinical improvement, progressive wound healing, and marked reductions in infection markers. The case highlights the decisive role of advanced sequencing technologies in identifying rare pathogens within polymicrobial infections, where conventional methods such as MALDI-TOF mass spectrometry may fail. It also emphasizes the importance of integrating precision diagnostics with surgical intervention, targeted antimicrobial therapy, and rigorous postoperative care to achieve successful outcomes. By documenting this unusual presentation, we aim to expand clinical awareness of W. chitiniclastica infections and provide a practical framework for managing similarly complex diabetic foot infections.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stacking Ensemble Approach for Predicting Loan Approval Using Machine Learning Techniques. 使用机器学习技术预测贷款批准的堆叠集成方法。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-23 DOI: 10.3791/68832

Kunchakara Raja Sekhar, Shaiku Shahida Saheb

{"title":"Stacking Ensemble Approach for Predicting Loan Approval Using Machine Learning Techniques.","authors":"Kunchakara Raja Sekhar, Shaiku Shahida Saheb","doi":"10.3791/68832","DOIUrl":"https://doi.org/10.3791/68832","url":null,"abstract":"Digital lending and fintech innovations have upended established banking systems, changing financial inclusion and credit availability in nations around the world. This study examines how peer-to-peer (P2P) and digital lending platforms are changing, emphasizing how technologies like artificial intelligence and machine learning are changing the way loans are approved. A thorough study of the literature highlights the opportunities and problems in the digital lending ecosystem, such as algorithmic risk assessment, customer trust, financial exclusion, and regulatory loopholes. This paper suggests a strong machine learning approach that uses a stacking ensemble model to accurately forecast loan approvals in order to address these issues. The data was pre-processed using train-test partitioning, exploratory analysis, and label encoding using a publicly accessible Kaggle dataset that included applicant demographics, financial characteristics, and credit histories. With XGBoost serving as the meta-learner, the ensemble incorporates the Gradient Boosting Model, Efficient Gradient Boosting, AdaBoost, and Extra Trees classifiers as base learners. With an accuracy of 98%, the model was assessed using measures including accuracy, precision, recall, F1-score, and error metrics (MAE- Mean Absolute Error, MSE- Mean Squared Error, and RMSE- Root Mean Square Error). According to correlation studies, factors including assets, income, and CIBIL scores have a significant impact on loan approvals. Outperforming conventional methods, the model showed balance and generalization across both classes. The usefulness of these models for automated, data-driven credit determinations is emphasized in the paper's conclusion.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conservative Management of Lip Avulsion with Hydrocolloid Dressings in Three Cases. 水胶体敷料保守治疗唇部撕脱3例。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-23 DOI: 10.3791/68762

Jiayi Yin, Min Wu, Ni Zeng, Qian Zheng, Bing Shi, Hanyao Huang

{"title":"Conservative Management of Lip Avulsion with Hydrocolloid Dressings in Three Cases.","authors":"Jiayi Yin, Min Wu, Ni Zeng, Qian Zheng, Bing Shi, Hanyao Huang","doi":"10.3791/68762","DOIUrl":"https://doi.org/10.3791/68762","url":null,"abstract":"Lip avulsion is a common orofacial trauma that poses challenges due to its aesthetic and functional implications. This retrospective case series evaluated the outcomes of three patients with partial-thickness traumatic lip avulsion managed using different wound healing approaches. Two patients underwent conservative treatment (secondary intention healing), including daily wound cleansing and application of hydrocolloid dressings. The hydrocolloid dressings could maintain a moist wound environment that promoted granulation formation and epithelialization while reducing infection risk. Both conservatively managed patients achieved favorable cosmetic and functional outcomes. The third patient initially underwent immediate wound suturing (primary intention healing), followed by wound dehiscence, necessitating subsequent conservative management. This patient developed noticeable scar contracture and distortion of the vermilion border. Our findings suggest that clinicians should carefully evaluate wound characteristics, contamination risks, and patient-specific factors when determining the initial management approach. Besides, conservative management with hydrocolloid dressings represents an effective, convenient, and practical therapeutic option for treating traumatic partial-thickness lip avulsion injuries.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0