Jove-Journal of Visualized Experiments最新文献_第3页

Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment. 用于犬鼻腔免疫微环境采样的生理盐水洗液

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2024-12-27 DOI: 10.3791/67577

Mary-Keara Boss, Braden Burdekin, Lauren Harrison, Madison Rivera, Adam Harris, Emily Rout, Erin Trageser, Patricia Gualtieri, Ber-In Lee, Steven Dow

引用次数: 0

Single Incision Plus One Port Laparoscopic Proximal Gastrectomy with Double Channel Anastomosis for Gastric Cancer Treatment. 单切口加一孔腹腔镜胃近端切除术双通道吻合治疗胃癌。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2024-12-27 DOI: 10.3791/67447

Ran Dou, Shengzhe Zuo, Yongkang Zhang, Xiaofeng Liao

{"title":"Single Incision Plus One Port Laparoscopic Proximal Gastrectomy with Double Channel Anastomosis for Gastric Cancer Treatment.","authors":"Ran Dou, Shengzhe Zuo, Yongkang Zhang, Xiaofeng Liao","doi":"10.3791/67447","DOIUrl":"https://doi.org/10.3791/67447","url":null,"abstract":"Single-incision plus one-port laparoscopic proximal gastrectomy with double-channel anastomosis (SILT-DT) is a minimally invasive surgical approach for treating proximal gastric cancer. This technique includes comprehensive laparoscopic resection of the proximal stomach, lymph node dissection, and double-tract anastomosis. By integrating single-port laparoscopic surgery with an auxiliary operating hole, SILT-DT reduces procedural difficulty while facilitating the placement of an abdominal drainage tube. Compared to the traditional five-port laparoscopic gastrectomy, SILT-DT requires fewer ports and results in shorter incision lengths, contributing to reduced postoperative pain and faster recovery. A thorough preoperative evaluation was conducted to ensure procedural success, with factors such as tumor size, stage, location, and patient BMI carefully considered. This comprehensive assessment allowed for optimal patient selection and surgical planning. Postoperative follow-up demonstrated no significant complications, underscoring the safety and efficacy of SILT-DT. This innovative technique offers a promising minimally invasive alternative for managing proximal gastric cancer while maintaining excellent clinical outcomes.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 214","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inducing the Entry of Third Stage Dispersal Juveniles of Bursaphelenchus xylophilus into Cryptobiosis Through Osmotic Regulation. 渗透调控诱导木桐第三期扩散幼虫进入隐生。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2024-12-27 DOI: 10.3791/67350

Long Pan, Donglin Wang, Yutian Suo, Yaqi Wu, Wenyi Zhao, Jian Chen, Zhen Yu, Jiaping Zhao

{"title":"Inducing the Entry of Third Stage Dispersal Juveniles of Bursaphelenchus xylophilus into Cryptobiosis Through Osmotic Regulation.","authors":"Long Pan, Donglin Wang, Yutian Suo, Yaqi Wu, Wenyi Zhao, Jian Chen, Zhen Yu, Jiaping Zhao","doi":"10.3791/67350","DOIUrl":"https://doi.org/10.3791/67350","url":null,"abstract":"Cryptobiosis is a state where organisms lose nearly all their internal water and enter anhydrobiosis under extreme environmental stress. The dispersal third-stage juveniles (pre-dauer juveniles, ) of Bursaphelenchus xylophilus can enter cryptobiosis through dehydration and revive upon rehydration when environmental conditions improve. Osmotic regulation is crucial for their survival in this process. In this study, specimens of B. xylophilus were collected from dead Pinus massoniana due to pine wilt disease in Ningbo, Zhejiang Province, China. Following immersion in 8% potassium chloride (KCl) solution, B. xylophilus entered a cryptobiotic state after gradual dehydration due to increased external osmotic pressure by natural water evaporation. B. xylophilus could resist low-temperature stress at -20 °C. B. xylophilus could revive upon rehydration, with a survival rate of 92.1%. This process can regulate the entry of B. xylophilus into cryptobiosis, enabling them to resist extreme environments. This method described in our study is simple and reliable, providing technical support for studying the stress resistance mechanisms of B. xylophilus.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 214","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasmid Stability Analysis with Open-Source Droplet Microfluidics. 开源液滴微流体质粒稳定性分析。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2024-12-27 DOI: 10.3791/67659

Pierre Padilla-Huamantinco, Emerson Durán, Tobias Wenzel

{"title":"Plasmid Stability Analysis with Open-Source Droplet Microfluidics.","authors":"Pierre Padilla-Huamantinco, Emerson Durán, Tobias Wenzel","doi":"10.3791/67659","DOIUrl":"https://doi.org/10.3791/67659","url":null,"abstract":"Plasmids play a vital role in synthetic biology by enabling the introduction and expression of foreign genes in various organisms, thereby facilitating the construction of biological circuits and pathways within and between cell populations. For many applications, maintaining functional plasmids without antibiotic selection is critical. This study introduces an open-hardware-based microfluidic workflow for analyzing plasmid retention by culturing single cells in gel microdroplets and quantifying microcolonies using fluorescence microscopy. This approach allows for the parallel analysis of numerous droplets and microcolonies, providing greater statistical power compared to traditional plate counting and enabling the integration of the assay into other droplet microfluidic workflows. By using plasmids expressing fluorescent proteins alongside a non-specific fluorescent DNA stain, single colonies can be identified and differentiated based on plasmid loss or fluorescent marker expression. Notably, this advanced workflow, implemented with open-source hardware, offers precise flow control and temperature management of both the sample and the microfluidic chip. These features enhance the workflow's ease of use, robustness, and accessibility. While the study focuses on Escherichia coli as the experimental model, the method's true potential lies in its versatility. It can be adapted for various studies requiring fluorescence signal quantification from plasmids or stains, as well as for other applications. The adoption of open-source hardware broadens the potential for conducting high-throughput bioanalyses using accessible technology in diverse research settings.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 214","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formulation of Zinc-Based Nanomaterials using the Eucommia ulmoides Bark Extract and their Wound Healing Potential. 杜仲树皮提取物制备锌基纳米材料及其伤口愈合潜力研究。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2024-12-27 DOI: 10.3791/67416

Xinfa Liu, Jie Li, Durairaj Karthick Rajan, Shubing Zhang

引用次数: 0

Sound Source Localization Testing in Single-sided Deafness Following Bone Conduction Intervention. 单侧耳聋骨传导干预后声源定位测试。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2024-12-20 DOI: 10.3791/67300

Xiao Zhang, Jie Bai, Zhihan Lin, Ying Li, Haihong Liu, Yang Yang

{"title":"Sound Source Localization Testing in Single-sided Deafness Following Bone Conduction Intervention.","authors":"Xiao Zhang, Jie Bai, Zhihan Lin, Ying Li, Haihong Liu, Yang Yang","doi":"10.3791/67300","DOIUrl":"https://doi.org/10.3791/67300","url":null,"abstract":"Single-sided deafness (SSD), where there is severe to profound hearing loss in one ear and normal hearing in the other, is a prevalent auditory condition that significantly impacts the quality of life for those affected. The ability to accurately localize sound sources is crucial for various everyday activities, including speech communication and environmental awareness. In recent years, bone conduction intervention has emerged as a promising solution for patients with SSD, offering a non-invasive alternative to traditional air conduction hearing aids. However, the effectiveness of bone conduction devices (BCDs), especially in terms of improving sound localization abilities, remains a topic of considerable interest. Here, we present a protocol to assess the impact of bone conduction intervention on sound localization ability in patients with SSD. The protocol includes the experimental setup (a sound-treated room and a semicircular array of loudspeakers), stimuli, and data analysis methods. Participants indicate the perceived direction of noise bursts, and their responses are analyzed using root mean square error (RMSE) and bias. The results of sound localization testing before and after bone conduction intervention are reported and compared. Despite no significant differences, most patients (71%) had a localization bias clearly toward the intervention side after bone conduction intervention. The study concludes that bone conduction intervention can promptly enhance certain sound localization skills in patients with SSD, offering evidence to support the efficacy of BCDs as a treatment for SSD.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 214","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of EcoHIV-Infected Cells in Microglia-Manipulated Transgenic Mice. 小胶质细胞转基因小鼠中ecohiv感染细胞的鉴定

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2024-12-20 DOI: 10.3791/67150

Hailong Li, Makensie Walker, Hao Ji, Aliaksandra Sikirzhytskaya, Marina Aksenova, Michael Shtutman, Vitali Sikirzhytski, Charles F Mactutus, Rosemarie M Booze

{"title":"Identification of EcoHIV-Infected Cells in Microglia-Manipulated Transgenic Mice.","authors":"Hailong Li, Makensie Walker, Hao Ji, Aliaksandra Sikirzhytskaya, Marina Aksenova, Michael Shtutman, Vitali Sikirzhytski, Charles F Mactutus, Rosemarie M Booze","doi":"10.3791/67150","DOIUrl":"10.3791/67150","url":null,"abstract":"Combined antiretroviral therapy (cART) has dramatically improved the quality of life for people living with HIV (PLWH). However, over 4 million PLWH are over the age of fifty and experience accompanying HIV-associated neurocognitive disorders (HAND). To understand how HIV impacts the central nervous system, a reliable and feasible model of HIV is necessary. Previously, a novel biological system using chimeric HIV (EcoHIV) inoculation was developed in a rat model to investigate neurocognitive impairments and synaptic dysfunction. Nevertheless, a significant challenge remains in clarifying EcoHIV's neuroanatomical distribution, particularly its differential expression in various cell types in the brain. In the current study, EcoHIV with mScarlet fluorescence labeling was modified and retro-orbitally injected into Tmem119-EGFP knock-in mice (which express enhanced green fluorescence protein primarily in microglia) to determine if microglia are the major cell type responsible for viral expression and reservoirs of HIV in the brain. The current data show that: (1) in vitro, EcoHIV-mScarlet fluorescence signals were predominantly localized in microglia-like cells among primary rodent brain cells; (2) in vivo, injection of EcoHIV-mScarlet into Tmem119-EGFP mice induced significant HIV expression in the mouse brain. The co-localization of mScarlet and EGFP signals suggests that microglia are the main cell type harboring HIV in the brain. Overall, EcoHIV in rodents offers a valuable biological system to study microglial alterations, viral reservoirs in the brain, and the neurological mechanisms of HIV-associated neurocognitive disorders.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 214","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Laparoscopic Partial Splenectomy with Total Blood Flow Occlusion in Benign Splenic Lesions. 全血流阻断下腹腔镜脾部分切除术在脾良性病变中的应用。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2024-12-20 DOI: 10.3791/66254

Zihui Zhou, Huohui Ou, Jie Lin, Qingbo Liu, Weidong Wang

{"title":"Application of Laparoscopic Partial Splenectomy with Total Blood Flow Occlusion in Benign Splenic Lesions.","authors":"Zihui Zhou, Huohui Ou, Jie Lin, Qingbo Liu, Weidong Wang","doi":"10.3791/66254","DOIUrl":"https://doi.org/10.3791/66254","url":null,"abstract":"Laparoscopic partial splenectomy (LPS) is gradually becoming the preferred method for treating benign splenic lesions. However, due to the abundant blood supply and its soft, fragile tissue texture, especially when the lesion is located near the splenic hilum or is particularly large, performing partial splenectomy (PS) in clinical practice is extremely challenging. Therefore, we have been continuously exploring and optimizing hemorrhage control methods during PS, and we here propose a method to perform LPS with complete spleen blood flow occlusion. This study describes an optimized approach to control intraoperative hemorrhage during LPS. First, it involves the thorough dissection of the splenic ligaments and careful separation of the pancreatic tail from the spleen. With complete exposure to the splenic hilum, we temporarily occlude the entire blood supply of the spleen using a laparoscopic bulldog clip. Subsequently, we employ intraoperative ultrasound to identify the boundary of the lesion and resect the corresponding portion of the spleen under complete blood flow control. This approach embodies the essence of 'spleen preservation' through effective hemorrhage control and precise resection. It is particularly suitable for laparoscopic surgery and deserves further clinical promotion.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 214","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extended Live Imaging of Female Drosophila melanogaster Germline Stem Cell Niches. 雌性黑腹果蝇生殖系干细胞龛的扩展实时成像。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2024-12-20 DOI: 10.3791/67389

Helena Sánchez-Gómez, Juan Garrido-Maraver, Patricia Rojas-Ríos

引用次数: 0

Induction of Retinal Ischemia-Reperfusion Injury in a Mouse Eye Model. 小鼠眼模型视网膜缺血再灌注损伤的诱导。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2024-12-20 DOI: 10.3791/67030

Nayan Sanjiv, Tat Fong Ng, Andrew W Taylor

{"title":"Induction of Retinal Ischemia-Reperfusion Injury in a Mouse Eye Model.","authors":"Nayan Sanjiv, Tat Fong Ng, Andrew W Taylor","doi":"10.3791/67030","DOIUrl":"https://doi.org/10.3791/67030","url":null,"abstract":"Ischemia-reperfusion injuries are known to cause a range of retinal pathologies, including diabetic retinopathy, glaucoma, retinal vascular occlusions, and other vaso-occlusive conditions. This manuscript presents a method for inducing ischemia-reperfusion injury in a mouse model. The method utilized anterior chamber cannulation attached to a saline reservoir, generating hydrostatic pressure to raise the intraocular pressure to 90-100 mmHg. This method effectively caused constriction of retinal capillaries to induce retinal ischemia. At the end of the ischemic period (60 min), the intraocular pressure was normalized (≤20 mmHg) before removing the cannula from the anterior chamber to initiate reperfusion. Days after the ischemia/reperfusion procedure, the eyes were collected and sectioned for histological staining. The histopathology of the retinal sections was scored by evaluating eight parameters of retinal injury: folds, hemorrhage, deformation, cell loss in the ganglion cell, inner nuclear, outer nuclear, and photoreceptor layers, and damage to retinal pigment epithelial cells. This method provided a reproducible model to study the mechanisms and pathology of retinal ischemia/reperfusion injury. In addition, this model can facilitate the discovery of potential therapeutic targets to treat retinal ischemia/reperfusion injury, advancing the study of retinal pathologies and improving patient outcomes.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 214","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0