Jove-Journal of Visualized Experiments最新文献

{"title":"Material Performance and Environmental Response in Historic Masonry Buildings: A Multicenter Longitudinal Observational Study.","authors":"Yaolong Sun","doi":"10.3791/72951","DOIUrl":"10.3791/72951","url":null,"abstract":"<p><p>Adaptive reuse can extend the service life of historic buildings, but its performance must be evaluated without assuming that more intensive intervention causes better outcomes. This multicenter longitudinal observational study compared 72 historic masonry buildings in Guangzhou, Nanjing, and Xi'an managed under conservation-only, material-compatible passive retrofit, or adaptive reuse with reversible environmental control. Archived baseline-to-month-12 wall-moisture change was -0.23 ± 0.95, -2.09 ± 0.78, and -2.37 ± 0.86 percentage points, respectively. In the primary ANCOVA, strategy was associated with month-12 wall moisture (F(2,61) = 40.96, P < 0.001); adjusted differences were -1.88 points for passive retrofit versus conservation-only (95% CI, -2.40 to -1.36) and -2.20 for adaptive reuse versus conservation-only (95% CI, -2.73 to -1.67), while adaptive reuse and passive retrofit did not differ (-0.32; 95% CI, -0.84 to 0.20; Holm-adjusted P = 0.222). The monthly humidity ratio showed no independent association with strategy (P = 0.410). In a transparent reanalysis of all five questionnaire outcomes, respondent-rated thermal comfort (q = 0.0038), functional fit (q = 0.0110), and heritage authenticity (q = 0.0038) were associated with strategy, whereas perceived air quality and overall satisfaction were not (both q = 0.592). Archived thermal-comfort-hour percentages, soluble-salt percentages, and monthly wall-moisture summaries were not used for confirmatory inference because their underlying classification, extraction/conversion, or acquisition/calibration records were unavailable. The supported findings are adjusted observational associations and do not establish causation.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Hybrid Neutrosophic Deep Learning Framework with Multi-Convolutional Neural Network Feature Fusion for Robust Facial Recognition.","authors":"Dyala Ibrahim, Omar Islam Al Mrayat, Tamara Al-Shurbaji, Ashraf Alyanbaawi, Abdulqader M Almars, Easa Alalwany, Malik Almalki, Basemah Alshemali, El-Sayed Atlam Atlam, Ibrahim Gad","doi":"10.3791/72192","DOIUrl":"https://doi.org/10.3791/72192","url":null,"abstract":"<p><p>The performance of many facial recognition (FR) algorithms is adversely affected in uncontrolled settings due to diverse facial expressions, lighting changes, occlusions, and image noise. This poses a significant challenge in developing a reliable and accurate facial recognition system for real-world applications. Hence, this paper introduces a novel hybrid deep learning-based facial recognition framework aimed at overcoming the shortcomings of the current solutions, specifically, inaccuracy and poor computational efficiency caused by complex facial images. Several pre-trained CNN architectures (VGG16, EfficientNetB0, ResNet-50, MobileNet, MobileNetV2, Xception, InceptionResNetV2, and NASNetLarge) are utilized in the proposed solution. Eight CNN models were evaluated, and the four most effective were identified based on their performance metrics. The proposed hybrid system employs a feature-level fusion method in which deep features from the best-performing models are combined. Subsequently, a smoothing operation on the resultant feature vector was performed, and the output vector was flattened and concatenated for the classification stage. According to the experimental results, the proposed hybrid framework outperforms all the individual CNN models used in the study, achieving an accuracy rate of 96.9%, a precision of 94.1%, a recall of 100%, and an F1-score of 96.9%.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149216742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Admission Serum Homocysteine with Discharge Neurocognitive Dysfunction after Acute Cerebrovascular Disease in a High-Altitude Setting.","authors":"Nan Wang, Jialin Sun, Weizhong Ji","doi":"10.3791/73407","DOIUrl":"https://doi.org/10.3791/73407","url":null,"abstract":"<p><p>Discharge-phase neurocognitive abnormalities may affect rehabilitation participation, self-management, and discharge safety after acute cerebrovascular disease, but early clinically accessible correlates remain incompletely defined. We examined the association between admission serum homocysteine and clinically adjudicated discharge neurocognitive dysfunction in a high-altitude clinical setting. This single-center retrospective cohort included 227 adults admitted for acute ischemic stroke, intracerebral hemorrhage, or transient ischemic attack. The first available serum homocysteine measurement within 24 h of hospital arrival was analyzed continuously and by cohort-derived tertiles. The primary outcome was neurocognitive dysfunction documented within 24-48 h before discharge and adjudicated independently by two reviewers blinded to homocysteine levels; standardized cognitive instruments were not systematically available. Logistic regression was sequentially adjusted for demographic, renal, neurological, neuroimaging, and cerebrovascular phenotype-related factors. Among 227 patients, 98 (43.2%) had discharge neurocognitive dysfunction. Admission homocysteine was higher in patients with dysfunction than in those without dysfunction (18.3 ± 6.2 vs. 12.4 ± 4.7 µmol/L, P < 0.001), and dysfunction prevalence increased from 22.4% in T1 to 38.5% in T2 and 69.9% in T3 (P for trend < 0.001). In the primary fully adjusted model, each 1 µmol/L increase in homocysteine was associated with higher odds of dysfunction (OR 1.14, 95% CI 1.07-1.21, P < 0.001), and T3 versus T1 remained associated with the outcome (OR 4.36, 95% CI 2.00-9.51, P < 0.001). Estimates were similar in prespecified sensitivity analyses, including exclusion of delirium or positive confusion assessment method for the intensive care unit (CAM-ICU) screening and restriction to acute ischemic stroke. Higher admission homocysteine was associated with discharge-phase neurocognitive dysfunction, supporting prospective evaluation of homocysteine as a potential marker of short-term neurocognitive vulnerability rather than a validated predictor of persistent post-stroke cognitive impairment.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149216930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Standardized Workflow for Multimodal Assessment of Right-to-Left Shunt-Related Ischemic Brain Injury.","authors":"Yiwen Zhang, Songqi Hou, Zhenzhen Yu, Yanyan Huang, Weibin Chen","doi":"10.3791/72082","DOIUrl":"https://doi.org/10.3791/72082","url":null,"abstract":"<p><p>Right-to-left shunt (RLS) has been associated with cryptogenic stroke, migraine, and other neurological conditions in which ischemic brain injury may be clinically relevant. In routine practice, however, assessment is often fragmented. Shunt presence may be documented, but shunt burden, provoked versus non-provoked patterns, anatomical complexity, and MRI-defined brain injury are not always integrated into a reproducible workflow. Here, we describe a standardized protocol for multimodal assessment of RLS-related ischemic brain injury in a clinically selected cohort. The workflow includes patient screening, exclusion of major vascular and systemic factors that could independently contribute to brain parenchymal injury, contrast-enhanced transcranial Doppler ultrasound (c-TCD) confirmation and shunt grading, brain magnetic resonance imaging (MRI) classification, transthoracic and/or transesophageal echocardiographic anatomical assessment, structured data extraction, and dataset cleaning. To improve reproducibility, the protocol also standardizes key coding steps, including shunt-grade harmonization, classification of RLS as simple or complex, and interpretation of D-dimer values reported below the assay threshold. Using this workflow, a harmonized analytic cohort of 60 RLS-positive patients was established. MRI identified 23 MRI-negative and 37 MRI-positive patients. Representative analyses showed that MRI-positive findings were more frequent in patients with greater shunt burden and more complex anatomical phenotypes. This protocol provides an operational model for incorporating c-TCD, MRI, echocardiography, and structured clinical parameters into the evaluation of MRI-defined ischemic brain injury in RLS-positive patients. It should be interpreted as a workflow for standardized multimodal phenotyping rather than as a standalone physiologic assessment of all clinically significant or hypoxemia-related RLS.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149216856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Improvement Following Multimodal Treatment in Depersonalization-Derealization Disorder: A Case Report.","authors":"Qiyu Zheng, Yan Zhao, Sisi Zheng, Hong Zhu","doi":"10.3791/73632","DOIUrl":"https://doi.org/10.3791/73632","url":null,"abstract":"<p><p>Depersonalization-derealization disorder (DPD) is characterized by persistent or recurrent detachment from the self or the surrounding environment with preserved reality testing. Because established treatment evidence remains limited, individualized therapeutic strategies are often required. This report describes a patient with derealization, emotional blunting, poor memory, slowed reactions, and impaired attention. Psychiatric evaluation showed marked depersonalization-derealization symptoms, with a Cambridge Depersonalization Scale (CDS) score of 147, a 24-item Hamilton Depression (HAMD-24) Rating Scale score of 8, a 14-item Hamilton Anxiety (HAMA-14) Rating Scale score of 5, and a MATRICS Consensus Cognitive Battery (MCCB) composite T-score of 49. The patient underwent a stepwise multimodal treatment approach that included transcranial direct current stimulation, pharmacotherapy with duloxetine and tandospirone, duloxetine dose optimization, lamotrigine augmentation and titration, and later gradual medication tapering under follow-up monitoring. Clinical improvement was observed during longitudinal assessment. This case highlights the practical value of a monitored, sequential, and multimodal treatment strategy for a patient with DPD. Although the findings are limited to a single case, the observed temporal association between intervention and improvement provides a hypothesis-generating clinical observation and supports the need for systematic studies of multimodal treatment approaches in DPD.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149216881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brain-Protective Perioperative Care for Reducing Postoperative Cognitive Impairment in Older Adults: A Scoping Review.","authors":"Tingting Jiang, Muhammad AbuBakar Shabbir, Xinghua Ma","doi":"10.3791/73404","DOIUrl":"https://doi.org/10.3791/73404","url":null,"abstract":"<p><p>Postoperative cognitive impairment (POCI) occurs in 10-50% of older adults after surgery and is associated with increased morbidity and accelerated cognitive decline. The focus of perioperative prevention has expanded from opioid-sparing analgesia to broader brain-protective perioperative care; however, evidence for individual strategies remains variable, and cognitive outcome assessment is heterogeneous. This scoping review aimed to map perioperative interventions used to prevent POCI in older surgical patients and characterize how cognitive outcomes are defined, assessed, and prioritized. The review followed Joanna Briggs Institute methodology and PRISMA-ScR reporting guidance. Seven electronic databases were searched without a date restriction. Eligible studies included adults aged 60 years or older undergoing surgery and evaluated at least one perioperative strategy with a delirium or cognitive outcome. Twenty-three studies were identified across five strategy categories: opioid-sparing and multimodal analgesia; anesthetic agents and techniques; intraoperative neuromonitoring; physiological optimization; and multicomponent non-pharmacological care and pharmacological prophylaxis. Multicomponent non-pharmacological interventions showed the most consistent signal for reducing postoperative delirium, whereas findings for individual pharmacological and technical strategies were inconsistent. Long-term cognitive outcomes were infrequently evaluated. The evidence supports a whole-pathway approach to brain-protective perioperative care while highlighting important gaps in long-term outcome assessment, standardization of cognitive testing, and direct evaluation of cognitive outcomes associated with opioid-sparing strategies.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149216927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatoprotective Effects of N-Acetylcysteine and Guarana in a Wistar Rat Model of Acute Acetaminophen Toxicity.","authors":"Canan Sahin, Yahya Sahin, Ozlem Kara","doi":"10.3791/74110","DOIUrl":"https://doi.org/10.3791/74110","url":null,"abstract":"<p><p>Acetaminophen overdose causes acute liver injury through oxidative stress, inflammation, and hepatocellular damage. This study evaluated the post-exposure effects of N-acetylcysteine (NAC), guarana, and their combination in a rat model of acute acetaminophen toxicity. Thirty male Wistar rats were randomly allocated to the Control, APAP, APAP + NAC, APAP + Guarana, and APAP + NAC + Guarana groups. Acetaminophen (3 g/kg) was administered orally on day 8. Treatments were initiated 4 h later and repeated on days 9 and 10; NAC was administered intraperitoneally at 1.5 g/kg, whereas guarana was administered orally at 300 mg/kg. Serum liver enzymes, circulating oxidative stress and antioxidant parameters, hepatic TNF-α and IL-1β levels, and liver histopathology were assessed. Acetaminophen increased serum AST, ALT, ALP, and catalase activities, serum MDA levels, and hepatic TNF-α and IL-1β levels, decreased serum SOD activity, and caused marked histopathological injury. Histopathological scores differed significantly among groups for all six evaluated parameters and the total liver injury score (all P < 0.001). NAC monotherapy improved selected biochemical and inflammatory outcomes and significantly reduced sinusoidal congestion, inflammatory cell infiltration, and architectural disruption relative to APAP. Guarana monotherapy showed numerical changes in several measures but did not differ significantly from APAP in the histopathological comparisons after multiplicity correction. Combined NAC and guarana treatment produced the broadest treatment-associated response and significantly lower histopathological scores than APAP across all evaluated parameters. These findings support further evaluation of guarana as a potential adjunct to NAC after acetaminophen exposure without establishing superiority over NAC or a synergistic interaction.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Throughput Spot Assay for Isolation of Bacteriophages Against Multiple Host Strains from Environmental Samples.","authors":"Xiaofu Wan, Yukun Zeng, Shuhong Han, Zixun Lin, Xiangke Duan, Min Ming, Wenheng Gan, Hongzhou Lu, Jiayin Shen","doi":"10.3791/73287","DOIUrl":"https://doi.org/10.3791/73287","url":null,"abstract":"<p><p>Phage therapy has emerged as a promising option for treating infections caused by multidrug-resistant bacteria, offering an alternative to conventional antibiotics. However, conventional isolation methods are laborious and tend to favor dominant host-phage pairs, limiting the diversity of recoverable phages. Expanding the collection of phages is therefore a critical step toward advancing phage-based therapies. To achieve effective isolation outcomes, it is essential to systematically screen environmental samples against a broad range of host strains under standardized conditions. The efficiency of phage recovery depends on several factors, including enrichment strategies, host range coverage, and infection dynamics. To address these challenges, we have developed a streamlined protocol that integrates liquid culture enrichment, polyethylene glycol (PEG)-mediated concentration, high-throughput spot screening on multiple host bacterial lawns, and subsequent streak purification for clonal isolation. This approach enables simultaneous screening of environmental samples against multiple pathogens in a single experimental setup, improving isolation efficiency. Notably, it enhances the recovery of phages with diverse host ranges. Our integrated workflow can reduce manual effort and material consumption and shorten the isolation period compared with conventional methods. This robust and evidence-based method accelerates the discovery of phages with potential applications in therapy and biocontrol. We anticipate that this protocol will prove particularly useful for building phage biobanks, preparing personalized phage therapeutics, and conducting large-scale environmental phage mining. Ultimately, it will help expand the repertoire of characterized phages for combating antimicrobial-resistant bacterial infections.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interspecific Somatic Cell Nuclear Transfer Using Bovine (Bos taurus) Zona Pellucida-Free Oocytes and Deer (Mazama americana) Fibroblasts.","authors":"Irving Mitchell Laines-Arcce, Dionet Keny Bellido-Quispe, Natanael Aguiar Braga Negreiros, Larissa Linhares Teixeira, Satish Kumar, Dárcio Ítalo Alves Teixeira, Pietro Sampaio Baruselli, Luciana Magalhães Melo, Vicente José Figueirêdo Freitas","doi":"10.3791/72989","DOIUrl":"https://doi.org/10.3791/72989","url":null,"abstract":"<p><p>Reproductive biotechnologies used in animals make it possible to increase species populations with high genetic value. Furthermore, these technologies aid in the conservation, preservation, and dissemination of genetic material from individuals with superior characteristics. The genetic rescue of selected individuals has led to numerous studies in domestic species aiming to extrapolate the findings to other animal populations or specific individuals. One such approach is through the use of somatic cell nuclear transfer (SCNT), or cloning, which is used to produce embryos with genetic material identical to that of an existing specimen. Among the limitations of this technique is the restricted availability of oocytes from wild animals. Thus, as an alternative, interspecific somatic cell nuclear transfer (iSCNT) emerged using a somatic cell (karyoplast) from the species to be cloned as a nuclear donor and its insertion into an enucleated oocyte (cytoplast) obtained from a species belonging to a different order or taxon. This approach makes it possible to obtain viable cloned embryos. In Brazil, there are nine species of deer, and many of them are endangered due to indiscriminate hunting, habitat loss, and other causes. The red brocket deer (Mazama americana) is one of the species suffering from these conditions. Therefore, this work presents a methodology for obtaining cloned embryos from red brocket deer using the iSCNT technique, in order to establish an embryo bank for subsequent transfer to recipient females and thereby help ensure the survival of the species.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Qu's Formula 1 for Anxiety and Depression in PCOS: A Computational Network Pharmacology, Molecular Docking, and Molecular Dynamics Study.","authors":"Chang Liu, Weihuan Hu, Jue Zhou, Fangfang Wang, Runju Zhang, Fan Qu","doi":"10.3791/73697","DOIUrl":"https://doi.org/10.3791/73697","url":null,"abstract":"<p><p>Qu's Formula 1 (QUF1), a patented Chinese herbal medicine, has shown clinical potential in alleviating anxiety and depression in patients with polycystic ovary syndrome (PCOS). This study aimed to investigate potential molecular mechanisms associated with QUF1 using network pharmacology, molecular docking, and molecular dynamics (MD) simulations. Active constituents and potential therapeutic targets of QUF1 were identified using TCMSP and BATMAN-TCM, while PCOS-, anxiety-, and depression-related targets were retrieved from GeneCards, OMIM, and DrugBank. Overlapping targets were used to construct a herb-constituent-target network, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses to identify relevant pathways. Molecular docking and 100 ns MD simulations were subsequently used to assess predicted interactions and structural stability between selected constituents and key targets. A total of 76 common targets were identified at the intersection of QUF1 and the disease-related target sets. Protein-protein interaction (PPI) analysis highlighted ALB, TNF, AKT1, IL-6, and ESR1 as key network targets, while KEGG enrichment identified the PI3K-Akt, HIF-1, and FoxO signaling pathways among the relevant pathways. Molecular docking and MD simulations further indicated favorable predicted interactions and generally stable structural behavior for the selected compound-target complexes. These findings support a computational hypothesis that QUF1 may act through a multi-compound, multi-target network associated with inflammatory regulation in PCOS-related anxiety and depression. The results provide a theoretical framework for subsequent experimental and pharmacokinetic validation.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}