Jove-Journal of Visualized Experiments最新文献_第4页

Transmesenteric Laparoscopic Pyeloplasty in Trendelenburg Position for Horseshoe Kidney with Hydronephrosis. 经肠系膜腹腔镜肾盂成形术在Trendelenburg位治疗马蹄肾伴肾积水。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-07-08 DOI: 10.3791/68425

Zhaohui Chen, Ming Xiong, Ruixiang Dai, Huiling Jiang, Yongqiang Wang, Song Wu, Teng Hou

{"title":"Transmesenteric Laparoscopic Pyeloplasty in Trendelenburg Position for Horseshoe Kidney with Hydronephrosis.","authors":"Zhaohui Chen, Ming Xiong, Ruixiang Dai, Huiling Jiang, Yongqiang Wang, Song Wu, Teng Hou","doi":"10.3791/68425","DOIUrl":"10.3791/68425","url":null,"abstract":"Due to anomalous anatomy, pyeloplasty with a horseshoe kidney (HSK) presents a technical challenge. Previously, we reported a technique of intraperitoneal laparoscopic surgery in Trendelenburg position (ILSTP) for pyelolithotomy, pyeloplasty, and heminephrectomy in patients with HSKs. To demonstrate the procedure, the case of a 29-year-old female with HSK and left hydronephrosis who underwent pyeloplasty using transmesenteric ILSTP is presented. Magnetic resonance confirmed HSK with left-sided hydronephrosis. After opening a window in the mesentery and dissecting the renal pelvis and left ureter, a crossing vein causing ureteropelvic junction obstruction (UPJO) was identified and ligated, followed by non-dismembered pyeloplasty. The operative time was 147 min with blood loss of 5 mL. Follow-up evaluation at 8 weeks post-stent removal demonstrated significant resolution of hydronephrosis without complications. This study describes the feasible use of the transmesenteric ILSTP technique, providing an important method to perform pyeloplasty in selected HSK patients. This technique requires surgeons to possess advanced laparoscopic skills, and the method may face limitations depending on the thickness of the mesentery.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 221","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Behavioral Characterization of Pentylenetetrazole-induced Seizures: Moving Beyond the Racine Scale. 戊四唑诱发癫痫的行为特征：超越拉辛量表。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-07-08 DOI: 10.3791/68112

Antonio Jhones Rocha, Ana Augusta C Rangel, Claudio Marcos Queiroz

{"title":"Behavioral Characterization of Pentylenetetrazole-induced Seizures: Moving Beyond the Racine Scale.","authors":"Antonio Jhones Rocha, Ana Augusta C Rangel, Claudio Marcos Queiroz","doi":"10.3791/68112","DOIUrl":"10.3791/68112","url":null,"abstract":"Detailed behavioral characterization of seizures is essential in animal models of epilepsy. Although various protocols exist for inducing seizures, the Racine scale remains the most widely adopted method for its quantification. Initially developed to score the progressive recruitment of behaviors during electrical kindling of limbic structures, the scale has since become the standard for classifying seizures across models with varying etiologies. However, careful examination of behavior following pentylenetetrazole (PTZ) administration, a commonly used seizure model, reveals that the Racine scale cannot capture the full spectrum of behavioral alterations induced by this GABAergic antagonist. After a single subcutaneous high-dose PTZ injection, mice first exhibit non-convulsive behaviors such as hypotonia, reduced ambulation, behavioral arrest, and tremors. These are succeeded by ictal behaviors, including spasms, myoclonic jerks, and limbic seizures, though only the latter is quantified by the Racine scale. To overcome these limitations, a refined approach is employed that combines video tagging with animal tracking to distinguish spasms and myoclonus as distinct behavioral expressions from limbic seizures. These early behaviors may facilitate the recruitment of limbic structures, eventually triggering limbic seizures. The method is straightforward to implement and enables a more comprehensive assessment of PTZ-induced acute seizures. Its utility is further evident in the PTZ kindling protocol, in which repeated low doses of PTZ result in temporal changes in spasms, myoclonus, and limbic seizure severity. This approach holds potential for improving reproducibility in seizure and epilepsy research.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 221","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR. 利用超极化13C核磁共振实时检测活细胞代谢。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-07-08 DOI: 10.3791/68539

Tomoto Ura, Keita Saito, Ryoma Kobayashi, Natsuko Miura, Yoichi Takakusagi

{"title":"Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR.","authors":"Tomoto Ura, Keita Saito, Ryoma Kobayashi, Natsuko Miura, Yoichi Takakusagi","doi":"10.3791/68539","DOIUrl":"10.3791/68539","url":null,"abstract":"The protocol presents a method to integrate an in-house-built bioreactor system with hyperpolarized 13C nuclear magnetic resonance (NMR) spectroscopy to measure pyruvate metabolism in live cells in real time. The protocol outlines the construction of a bioreactor system that allows precise timing of hyperpolarized substrate injection and NMR signal acquisition while maintaining a stable cell culture environment. The method reliably detects the metabolic conversion of pyruvate to lactate within a 60 s measurement window while maintaining a sufficient signal-to-noise ratio throughout the process. The in situ mixing capability of the system accelerates NMR signal detection and reduces T1 relaxation losses. The protocol also includes detailed instructions for the preparation of alginate gels to encapsulate cells and place them within the bioreactor. The proposed method exhibits improved reproducibility and kinetic accuracy compared to conventional methods, indicating potential applications in cancer metabolism studies and drug screening. Further refinements of the system, such as the adoption of alternative gel matrices, may improve its versatility in various biological studies.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 221","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subcutaneous Injection of Human Colorectal Cancer Cells in Athymic Nude Mice to Evaluate Antitumor Efficacy. 胸腺裸鼠皮下注射人结直肠癌细胞的抗肿瘤效果评价。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-07-08 DOI: 10.3791/68525

Andreia-Filipa Cruz, Clara Noguera-Navarro, Begoña Alburquerque-González, Juan Cabezas-Herrera, Silvia Montoro-García

{"title":"Subcutaneous Injection of Human Colorectal Cancer Cells in Athymic Nude Mice to Evaluate Antitumor Efficacy.","authors":"Andreia-Filipa Cruz, Clara Noguera-Navarro, Begoña Alburquerque-González, Juan Cabezas-Herrera, Silvia Montoro-García","doi":"10.3791/68525","DOIUrl":"10.3791/68525","url":null,"abstract":"Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths worldwide, highlighting the urgent need for improved preclinical models to evaluate potential therapies. Animal models are essential for elucidating tumor biology and assessing novel therapeutic interventions. Mouse models offer key advantages over existing models, including genetic manipulability, rapid reproduction, cost-effectiveness, and the availability of immunocompetent and immunodeficient strains, enabling precise modeling of human cancers. This protocol focuses on the subcutaneous induction of the bioluminescent colorectal cancer cell line HCT-116 in an immunodeficient murine model, followed by non-invasive bioluminescence imaging using the IVIS system and caliper to monitor tumor progression and growth. Using luminescent cells provides a real-time, quantifiable approach for tracking tumor development and response to therapeutic interventions, reducing the need for invasive procedures. A cohort of mice (n = 12) is required for tumor cell implantation, and luminescence intensity is measured to establish a standardized model for therapeutic testing. Tumor volume is typically calculated using only length and width measurements or width. The current methodology ensures consistent tumor establishment while minimizing° variability, ultimately enhancing the reliability of preclinical assessment. The findings of this study will contribute to refining tumor models for drug testing and improving the translational potential of preclinical research in clinical applications. By employing advanced imaging techniques and a reproducible experimental design, this study aims to improve the accuracy and efficiency of in vivo drug evaluation for colorectal cancer treatment.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 221","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis. 利用DNA荧光发光适体作为序列特异性核酸分析信号报告的分裂杂交探针。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-07-08 DOI: 10.3791/68483

AnnaMarie Knowles, Justine Monsalve, Yulia Gerasimova

{"title":"Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis.","authors":"AnnaMarie Knowles, Justine Monsalve, Yulia Gerasimova","doi":"10.3791/68483","DOIUrl":"10.3791/68483","url":null,"abstract":"DNA-based fluorescent light-up aptamers (FLAPs) are promising for bioanalytical assays because they provide a low-cost fluorescent signal readout without the need for labeling of nucleic acid signal reporters with fluorophores and/or quenchers, unlike conventional hybridization probes used for instantaneous nucleic acid detection. Instead, FLAPs non-covalently bind dye ligands , which exhibit intrinsically low fluorescence in aqueous solutions, but become highly emitting upon FLAP binding. This protocol describes an algorithm to design split light-up aptamer sensors (SLASs) utilizing DAP-10-42, the most efficient DNA FLAP reported thus far. When equipped with nucleic acid sequences complementary to a nucleic acid target of interest, SLAS is a promising tool for nucleic acid analysis allowing for the sequence-specific detection of nucleic acid targets with selectivity down to one nucleotide to enable analysis of single-nucleotide substitutions (SNSs). SLASs offer the advantage of a label-free fluorescence-based signal readout, which can be measured with a conventional cuvette-based fluorescent spectrophotometer, a portable fluorometer, or visually observed upon excitation with a handheld light source. The SLAS approach is beneficial for biosensing applications in disease diagnostics, environmental monitoring, and biomolecular research.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 221","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surgical Correction for Pediatric Epiblepharon and Trichiasis. 小儿上睑下垂及倒睫的手术矫正。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-07-08 DOI: 10.3791/67836

Yu Cui, Fengting Liu, Jiayu Xue

{"title":"Surgical Correction for Pediatric Epiblepharon and Trichiasis.","authors":"Yu Cui, Fengting Liu, Jiayu Xue","doi":"10.3791/67836","DOIUrl":"10.3791/67836","url":null,"abstract":"Lower eyelid epiblepharon is a condition that is common in Asian children and improves with age in most patients; however, the problem can persist until school age or even adulthood. No difference has been observed in terms of incidence in association with sex or the affected eye, and the condition mainly affects the lower eyelid, usually in both eyes. The condition typically features horizontal semilunar skin folds lying parallel to the palpebral margin that accumulate and push the eyelashes towards the ocular surface, which rub against the cornea, resulting in corneal injury and irritation symptoms that affect the daily life of the child. Surgery remains the currently preferred method for alleviating corneal irritation symptoms. Although diverse surgical procedures are available in terms of clinical practice, the modified Hotz procedure remains the most widely used. The surgical procedure primarily focuses on two key aspects: the precise determination of the amount of skin to be removed and improving the tightness between the palpebral retractor aponeurosis and the anterior layer of the eyelid. In this study, lower eyelid epiblepharon is categorized into three distinct types based on the affected area: medial lower eyelid epiblepharon (affecting the medial skin of the lower eyelid); central epiblepharon (involving half of the lower eyelid); and total involvement Type A epiblepharon (involving the full-length of the eyelid). The surgical approach was tailored according to classification, with Type A cases receiving L-shaped excisions and Types B and C crescent-shaped incisions. The postoperative outcomes were evaluated to determine the cure rate. Most patients experienced mild postoperative reactions, and no obvious sunken scars or double lower eyelids were observed. Satisfactory results were thus obtained with mild postoperative reactions.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 221","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication of Ultra-sharp Polymeric Microneedles via Computer Numerical Control (CNC) Micromachining and Micromolding. 利用计算机数控（CNC）微加工和微成型制造超锋利聚合物微针。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-07-08 DOI: 10.3791/68660

Tien Dat Nguyen, Hong-Phuc Pham, Long Binh Vong, Thanh-Qua Nguyen

{"title":"Fabrication of Ultra-sharp Polymeric Microneedles via Computer Numerical Control (CNC) Micromachining and Micromolding.","authors":"Tien Dat Nguyen, Hong-Phuc Pham, Long Binh Vong, Thanh-Qua Nguyen","doi":"10.3791/68660","DOIUrl":"10.3791/68660","url":null,"abstract":"Microneedles have recently gained recognition as a promising method in transdermal drug delivery owing to their minimally invasive, painless nature, and they can be flexibly engineered through geometric modifications to meet specific therapeutic requirements. Hence, this study aims to develop microneedle master molds utilizing Computer Numerical Control (CNC) milling technology to create polymeric microneedles with diverse degrees of sharpness to enhance therapeutic efficacy. Previously, our team successfully optimized two critical machining parameters, feed rate, and ramp angle, while analyzing their influence on the obtained needles. Hence, this article presents a comprehensive set of experimental protocols for fabricating and evaluating the performance of the polymeric-based model needles. Furthermore, this work also provides representative results to demonstrate the reproducibility and therapeutic efficacy of the sample needles. Our research team anticipates that this straightforward protocol will enable researchers to create cost-effective, high-precision microneedles, thereby advancing microneedle technology for further biomedical applications, particularly during the pandemic.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 221","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Modified Mirror Test as a Visual Guide for the Self-awareness Trait in Wild Antarctica Penguins, Pygoscelis adeliae. 一种改进的镜像测试作为野生南极企鹅自我意识特征的视觉指南。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-07-08 DOI: 10.3791/65927

Prabir G Dastidar, Azizuddin Khan, Anindya Sinha

{"title":"A Modified Mirror Test as a Visual Guide for the Self-awareness Trait in Wild Antarctica Penguins, Pygoscelis adeliae.","authors":"Prabir G Dastidar, Azizuddin Khan, Anindya Sinha","doi":"10.3791/65927","DOIUrl":"10.3791/65927","url":null,"abstract":"In this study, the self-awareness trait was tested in Adelie penguins living on a remote island in Antarctica. Four experimental paradigms were designed for the study to visualize the effect of a coordinated and networked social life on generating the self-awareness trait in Adelie penguins. The study, conducted in extremely cold, snow-covered, and difficult terrains of Antarctica during January-February 2020, reports the presence of traits of self-awareness in wild Adélie penguins in their natural habitat on Dog's Neck Ice Shelf and Svenner Island in East Antarctica. The study is based on a close and careful watch on their daily movements, social activities, and overall lifestyle. Besides, it reports reactions and postures of penguins in response to their images in mirrors during four experimental paradigms-group-behavior test, modified mirror test (MMT), hidden-head test, and colored-bib test. The paper argues that these experimental paradigms are probably the first attempt to find the presence of self-awareness traits in any penguin species. It is a pioneering attempt in conducting a set of cognitive experiments on the free-ranging individuals of a nonhuman species in their natural environment, without any prior familiarization, conditioning, or acclimatization of the experimental species. It is postulated that experimenting in a remote natural habitat, without any external superimposition, brings out the intrinsic qualities of an animal, in perfect equilibrium with its surroundings. Future studies integrating the socio-ecology and cognitive ethology of penguins may provide further insights into whether the reported self-awareness traits have evolved due to the cooperative behavior of individual penguins with conspecific individuals throughout their colonial/social setups. It is based on the understanding that self-awareness is an essential social requirement for a cooperative lifestyle and lies at the aggregated level of social cognition.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 221","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins. I TASSER, trRosetta, UCSF Chimera， HADDOCK server和HEX loria在蛋白质从头设计和芯片设计中的应用。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-07-08 DOI: 10.3791/68003

Jimena Ramírez-Villarreal, Roberto Álvarez-Martínez

引用次数: 0

En face Cryosectioning of Mouse Retina for High-dimensional Spatial Molecular Analysis. 小鼠视网膜面部冷冻切片用于高维空间分子分析。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-07-08 DOI: 10.3791/68171

Samuel A Budoff, Alon Poleg-Polsky

{"title":"En face Cryosectioning of Mouse Retina for High-dimensional Spatial Molecular Analysis.","authors":"Samuel A Budoff, Alon Poleg-Polsky","doi":"10.3791/68171","DOIUrl":"10.3791/68171","url":null,"abstract":"The complex laminar structure of the retina presents significant challenges for comprehensive spatial molecular mapping. Current techniques for investigating tissue architecture and molecular interactions are limited by technical constraints that compromise spatial relationships or restrict molecular profiling. Existing methods like cross-sectioning, whole mount preparations, chemical or mechanical dissociations, and thick en face sections either disrupt tissue integrity, lose critical spatial context, or most often, are simply incompatible with high-dimensional spatial sequencing platforms. This study introduces a cryosectioning technique that produces thin (<20 µm) en face sections while preserving the spatial relationships essential for mapping retinal cell distributions. The method maintains RNA integrity and tissue architecture while generating sections compatible with high-throughput molecular analysis platforms. RNA integrity is maintained because this technique sections fresh frozen tissue, which manufacturers of spatial transcriptomic platforms officially support relative to postfixed tissue. We validate this approach using multiple molecular profiling methods, demonstrating successful integration with both manual RNAscope in situ hybridization (12 targets) and Xenium spatial sequencing (300 targets). The technique consistently preserves tissue architecture across samples while maintaining RNA quality suitable for these sensitive molecular applications. This methodological advancement enables new investigations into retinal cell type distributions, molecular gradients across laminae, and spatial aspects of retinal pathology. The technique's versatility and compatibility with modern spatial biology platforms provide a foundation for comprehensive molecular mapping of the retina's complex cellular organization.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 221","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0