Jove-Journal of Visualized Experiments最新文献

{"title":"Impacts of Chinese Olympic Athletes' Visit to Macao on Patriotism and Integrity Behaviors Among University Students Studying in Macao.","authors":"FatFai Ieong, TakHong Cheang, SioHong Teng","doi":"10.3791/72388","DOIUrl":"10.3791/72388","url":null,"abstract":"<p><p>In recent years, sports diplomacy, national identity building, and integrity education have become key trends in national governance in the new era. After major sports events, it has become a unique tradition for Olympic athletes to visit Hong Kong and Macao for exchanges. The principles of integrity, fairness, and self-discipline in sportsmanship are important ways to cultivate Macao's youth's sense of national identity and national spirit. This study, using media reports on the visits of Chinese Olympic athletes to Macao as a backdrop, adopted grounded‑theory methodology and conducted semi‑structured interviews with 27 Macao university students. It explored the formation mechanisms of their cognitions and behaviors related to patriotism and integrity. The study found that students could transform their thinking patterns and behaviors simply by browsing news clips and short videos, without personally participating in the athletes' exchange activities in Macao. Peer interaction on campus reinforced the internalization of values, while cultural compatibility within the context of Sino-Portuguese cultural integration enhanced symbolic identification. This research provides empirical evidence for conducting sports-oriented patriotism and integrity education (or anti-corruption education) for Macao youth under the \"One Country, Two Systems\" framework.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rethinking Urgency Cues: Scarcity Messaging, Credibility, and Purchase Intention in a Survey of Social Media Fruit Advertising.","authors":"Min Liang, Guosheng Hu","doi":"10.3791/72770","DOIUrl":"https://doi.org/10.3791/72770","url":null,"abstract":"<p><p>Scarcity appeals are widely used in social media advertising, yet their influence on purchase intention may involve both cognitive and affective mechanisms. This study examines the relationships between scarcity message intensity and purchase intention through perceived credibility and fear of missing out (FOMO), with consumer skepticism and household income level as moderators. Drawing on the Elaboration Likelihood Model, Signaling Theory, and the Persuasion Knowledge Model, survey data from 272 Chinese social-commerce users were analyzed using partial least squares structural equation modeling (PLS-SEM). Scarcity message intensity was positively associated with perceived credibility, contrary to the negative relationship proposed in Hypothesis 1, and was also positively associated with FOMO. Both perceived credibility and FOMO were positively associated with purchase intention. Although the direct relationship between scarcity message intensity and purchase intention was non-significant, both indirect pathways were positive and statistically significant, with the indirect effect through perceived credibility larger than that through FOMO. Consumer skepticism significantly moderated the scarcity-FOMO relationship, but in the direction opposite to that hypothesized; therefore, H6 was not supported. Household income did not significantly moderate the perceived credibility-purchase intention relationship, and H7 was not supported.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149216690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Robot-Assisted Gasless Trans-Hairline Excision of Submandibular Gland Tumors.","authors":"Tao Shi, Shaofei Ma, Cheng Miao, Grace Paka Lubamba, Bo Han, Chunjie Li, Longjiang Li, Lin Que, Guiquan Zhu","doi":"10.3791/72575","DOIUrl":"https://doi.org/10.3791/72575","url":null,"abstract":"<p><p>Benign submandibular gland tumors are usually resected through a transcervical incision, which provides direct exposure but leaves visible cervical scarring. Remote-access endoscopic techniques improve scar concealment but can be limited by two-dimensional visualization, assistant-dependent camera control, and rigid instrument collision in the narrow submandibular gland space. This article presents a standardized video protocol for robot-assisted submandibular gland excision via a gasless trans-hairline approach for benign tumors. The procedure begins with postauricular trans-hairline incision design, subplatysmal flap elevation, and mechanical retractor-based creation of a gasless working cavity. After robotic docking, a 3D endoscope and articulated instruments are used for sequential dissection of the superior, posterior, inferior, deep, and anterior glandular borders. Critical steps include protection of the marginal mandibular branch of the facial nerve, management of the facial vein and facial artery branches, exposure of the digastric and mylohyoid muscles, identification of the lingual nerve, division of submandibular ganglion branches, clipping of Wharton's duct, en bloc gland-and-tumor retrieval, hemostasis, drainage, and layered closure. Ten female patients with clinically benign submandibular gland tumors underwent this procedure. All operations were completed robotically without conversion. The mean operative time was 134.5 min ± 36.9 min, the mean estimated blood loss was 16.2 mL ± 7.6 mL, and the mean incision length was 5.6 ± 0.5 cm. Final pathology confirmed pleomorphic adenoma in all patients. No major vascular injury, postoperative marginal mandibular, lingual, or hypoglossal nerve dysfunction, hematoma, seroma, infection, or salivary fistula was observed during the available follow-up. This protocol provides a reproducible workflow for selected patients, although larger studies with longer follow-up are needed.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149216705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taohong Siwu Decoction-Based Targeted Drug Penetration Therapy for Deep Vein Thrombosis After Hip Fracture Surgery: A Retrospective Study.","authors":"Kemin Sun, Xianfeng Chen, Xinqiang Zhou, Junyu Zheng, Haibo Hu, Zhi Shen","doi":"10.3791/72100","DOIUrl":"https://doi.org/10.3791/72100","url":null,"abstract":"<p><p>Postoperative lower limb deep vein thrombosis (DVT) remains a common complication after hip fracture surgery, and complementary strategies targeting coagulation, inflammation, and venous circulation may provide additional preventive benefits. This retrospective cohort study included 86 patients who underwent hip fracture surgery between June 2023 and June 2024. Patients were assigned to a control group (n = 43), receiving standard low-molecular-weight heparin (LMWH) prophylaxis, or an observation group (n = 43), receiving additional Taohong Siwu Decoction-based targeted drug penetration therapy. The primary outcome was postoperative lower limb DVT within 10 days after surgery. Secondary outcomes included coagulation parameters, inflammatory markers, femoral venous blood flow velocities, postoperative pain, limb swelling, and treatment-related adverse events. DVT occurred in 1/43 patients (2.33%) in the observation group and 10/43 patients (23.26%) in the control group (Fisher's exact P = 0.007). In a parsimonious Firth penalized logistic regression model adjusting for age and baseline D-dimer, adjunctive therapy remained associated with a lower likelihood of postoperative DVT (adjusted odds ratio [OR] = 0.12, 95% confidence interval [CI]: 0.02-0.68, P = 0.017). After 10 days of treatment, the observation group had lower Faces Pain Scale-Revised scores and limb swelling, longer activated partial thromboplastin time and prothrombin time, lower D-dimer levels, higher common and superficial femoral vein blood flow velocities, and lower serum tumor necrosis factor-alpha and interleukin-6 levels than the control group (all P < 0.05). No statistically significant between-group difference was observed in overall adverse-event occurrence, and no severe treatment-related adverse events were recorded. Taohong Siwu Decoction-based targeted drug penetration therapy combined with standard anticoagulation was associated with a lower incidence of early postoperative DVT and favorable differences in coagulation, inflammatory, and venous hemodynamic parameters.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149216862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Macrophage Polarization-Related Biomarkers in Multiple Myeloma Using Integrated Bioinformatics.","authors":"Ran Wang, Jingwei Zhang, Jing Qi, Yizhi Jiang, Nana Wang, Jun Huang, Sisi Tang, Yuanfeng Wei, Yu Xiang, Hesheng He, Laiquan Huang, Zhongling Wei","doi":"10.3791/72728","DOIUrl":"https://doi.org/10.3791/72728","url":null,"abstract":"<p><p>Multiple myeloma (MM) is an incurable hematologic neoplasm with heterogeneous prognosis. Within the bone marrow microenvironment, macrophage polarization (MP) contributes to immune remodeling and may influence disease progression, yet MP-related prognostic biomarkers remain insufficiently characterized. In this study, we integrated transcriptomic data from UCSC Xena (n = 844) and GSE4581 (n = 414) to identify differentially expressed genes between immune-hot and immune-cold MM samples stratified by single-sample gene set enrichment analysis (ssGSEA)-derived immune scores. Weighted gene co-expression network analysis (WGCNA) and intersection with published MP-related genes yielded candidate genes, which were further refined using univariate Cox regression combined with least absolute shrinkage and selection operator (LASSO), eXtreme gradient boosting (XGBoost) and support vector machine recursive feature elimination (SVM-RFE) machine learning algorithms to construct a prognostic model. An eight-gene signature (LGALS3, IL2RG, ADAM8, MERTK, ACSS1, ARSK, CBX6 and SOCS3) was established, demonstrating acceptable discriminatory ability for 1-, 2-, and 3-year overall survival (AUC 0.666-0.736 in training cohort). Risk groups differed significantly in immune infiltration patterns, predicted drug sensitivity, and survival outcomes. A predicted competing endogenous RNA (ceRNA) network centered on MERTK and SOCS3 was also constructed. These findings identify eight MP-related hub genes as candidate prognostic biomarkers and provide a risk model with acceptable predictive performance, offering insights into the immune-metabolic interplay in MM and suggesting potential avenues for personalized therapeutic strategies.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic, Phenotypic, and Quantitative Analysis of Myoblast Fusion in Drosophila Embryos.","authors":"Zhi-Rong Ruan, Danqing Tong, Elizabeth H Chen","doi":"10.3791/73791","DOIUrl":"https://doi.org/10.3791/73791","url":null,"abstract":"<p><p>The fusion of mononucleated myoblasts into multinucleated syncytia is a fundamental process in skeletal muscle development. Drosophila melanogaster is a powerful, genetically tractable model for rapidly and efficiently assessing the function of candidate genes involved in this process. Here, we describe a comprehensive protocol with step-by-step instructions for analyzing the myoblast fusion phenotype in Drosophila embryos. This method includes mutant line selection for candidate gene, embryo collection and fixation, whole-mount immunostaining, developmental staging, confocal imaging, and phenotypic analysis. Myosin heavy chain (MHC) staining is used to visualize overall muscle morphology and to identify unfused mononucleated myoblasts. In parallel, Even-skipped (Eve) staining labels nuclei within a specific muscle fiber, enabling quantitative analysis of fusion extent by counting the nuclei incorporated into that fiber. Taken together, this protocol enables the readers to assess the mutant phenotypes of any genes of interest and evaluate their roles in myoblast fusion. This approach provides a robust framework for identifying and characterizing novel regulators of myoblast fusion.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental Protocols for Developing and Validating Explainable AI Models in Secure Healthcare Diagnostics.","authors":"Sonali Patil, Meghana Lokhande, Shweta Koparde, Sonali Kothari, Sudan Jha","doi":"10.3791/72412","DOIUrl":"https://doi.org/10.3791/72412","url":null,"abstract":"<p><p>Artificial Intelligence (AI) has drastically enhanced healthcare diagnostics with automated disease detection, predictive analytics, and intelligent systems for clinical decision support. Nevertheless, the black-box nature of deep learning models hampers interpretability, clinical trust, and regulatory acceptance and raises concerns about data privacy and cybersecurity. This paper presents comprehensive experimental frameworks for developing and evaluating explainable AI models for secure healthcare diagnostics. The suggested model combines responsible data management, multimodal preprocessing, secure deep learning models, explainable artificial intelligence (XAI) methods, and reproducible validation procedures in a single healthcare diagnostic pipeline. Methods of explaining, such as Grad-CAM, SHAP, LIME, and counterfactual analysis, were used to make the models more transparent and understandable for clinicians. At the same time, federated learning and differential privacy methods were used to securely manage highly sensitive patient data. Experimental evaluation has shown 96% classification accuracy, 94% sensitivity, and 95% specificity. These results were consistent in heterogeneous healthcare datasets. Compared to traditional black-box models, the explainability framework not only enhanced the interpretability of the results but also more accurately identified the regions relevant for diagnosis. Moreover, the secure federated learning architecture minimized privacy leakages without sacrificing the predictive performance and remained robust even when exposed to adversarial perturbations. An external evaluation also confirmed the reproducibility and generalizability of the protocols involved. This methodology offers a comprehensive and reliable foundation for deploying AI-powered diagnostics across multiple healthcare domains: radiology, pathology, oncology, cardiology, neurology, and precision medicine.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-Invasive and Cost-Effective Fecal Occult Blood Detection for Colon Cancer Prognosis in Murine Models.","authors":"Jennifer Ana Iden, Tamar Liron, Yankel Gabet","doi":"10.3791/68527","DOIUrl":"https://doi.org/10.3791/68527","url":null,"abstract":"<p><p>Fecal occult blood testing is a widely used diagnostic method for the early detection of colorectal cancer and the assessment of gastrointestinal pathologies in humans. For preclinical research purposes, we developed a cost-effective, non-invasive luminol-based chemiluminescent assay to detect fecal occult blood in murine feces that can function as a predictive tool, with sufficient sensitivity to correlate with disease severity. Fecal samples were collected and weighed, solubilized in a sodium hydroxide solution, then combined with luminol and hydrogen peroxide and immediately analyzed using a luminometer. Luminol reacts with the iron in hemoglobin to produce a measurable chemiluminescent signal; therefore, known hemoglobin concentrations from mouse blood, quantified using a hemoglobinometer, were used to generate a standard curve along with feces from naïve mice. The method was validated against standard Guaiac paper slide tests for accuracy. We demonstrated a strong correlation with disease severity metrics, including colonoscopy findings and tumor burden in multiple models of colon cancer, offering an accessible, non-invasive, and inexpensive alternative for routine monitoring of disease progression. The application of this technique can be broadened to other murine models of gastrointestinal pathologies, such as peptic ulcers, inflammatory bowel disease, Crohn's disease, and gastritis.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative Transcriptomic Analysis of Obesity-Associated Candidate Genes in Pediatric Adipose Tissue.","authors":"Aimei Miao, Lei Xu, Biying Chen, Fan Su, Jingxia Yang, Zhenglan Li, Shiqin Li, Junjun Du, Chenlin Ji","doi":"10.3791/72376","DOIUrl":"https://doi.org/10.3791/72376","url":null,"abstract":"<p><p>Tryptophan metabolism has been linked to pediatric obesity, although the transcriptomic evidence connecting obesity-associated genes with this pathway remains limited. Two public subcutaneous adipose tissue datasets, GSE205668 and GSE104815, were analyzed. GSE205668 included 26 obesity and 35 control samples, and GSE104815 included 4 obesity and 4 control samples. Differential expression screening, weighted gene coexpression network analysis (WGCNA), exploratory coexpression enrichment, immune score analysis, and regulatory network prediction were performed. Four candidate genes, APOL4, FASN, KRT79, and MACROD1, emerged from the exploratory intersection analysis. Their expression was higher in control samples than in obesity samples in both public datasets. Results derived from nominal p-value screens are interpreted as exploratory. The literature-derived tryptophan-related WGCNA score is considered separately from the canonical Kyoto Encyclopedia of Genes and Genomes (KEGG) tryptophan-metabolism pathway. These findings define obesity-associated transcriptomic candidates in pediatric adipose tissue and do not establish direct participation of the four genes in tryptophan metabolism or a causal role in obesity.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nociception Level-Guided Multimodal Analgesia with External Oblique Intercostal Plane Block in Laparoscopic Cholecystectomy.","authors":"Bilge Aslan, Sena Saricaoglu Oktem, Aysun Ersen Yungul, Mustafa Turan, Erdal Ozcan, Feray Aydin, Ayca Tuba Dumanli Ozcan, Levent Ozturk","doi":"10.3791/72672","DOIUrl":"10.3791/72672","url":null,"abstract":"<p><p>Effective perioperative analgesia remains essential in laparoscopic cholecystectomy (LC), where pneumoperitoneum produces the most intense nociceptive stimulus despite the minimally invasive nature of the procedure. Objective monitoring of nociception using the nociception level (NOL) index may optimize intraoperative opioid titration; however, its integration into multimodal analgesia (MMA) strategies incorporating regional techniques has not been adequately studied. The study was conducted using a double-blind design in which patients and the postoperative outcome assessor were blinded to group allocation, whereas the investigator performing the EOIP block was necessarily unblinded. This randomized controlled trial investigated whether NOL-guided MMA, including an external oblique intercostal plane (EOIP) block, improves intraoperative nociceptive control and early postoperative pain compared with conventional intravenous analgesia. Sixty-two ASA I-II adults undergoing elective LC were randomized to receive either an EOIP block with dexketoprofen and magnesium infusion or intravenous dexketoprofen alone. In both groups, remifentanil infusion was titrated according to predefined NOL thresholds, and anesthetic depth was maintained using BIS monitoring. NOL values during pneumoperitoneum were significantly lower in the MMA group (mean difference: 13.51; 95% CI: 4.68-22.34; p < 0.004), indicating superior attenuation of peak nociceptive responses. Emergence pain scores were also reduced in the MMA group (p < 0.001), whereas total remifentanil consumption did not differ between groups. Sevoflurane requirements and extubation times were significantly lower in the MMA group, suggesting a potential anesthetic-sparing effect. No differences were observed in postoperative opioid rescue, nausea/vomiting, or other adverse events. These findings demonstrate that NOL-guided MMA incorporating EOIP block enhances intraoperative nociceptive stability and improves early postoperative analgesia without increasing opioid use. Larger multicenter studies are warranted to confirm these results and further refine NOL-integrated multimodal analgesic strategies.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 236","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}