Jove-Journal of Visualized Experiments最新文献_第6页

Examination of Anatomical Features of Retinal Ganglion Cells Under N-methyl-D-aspartic Acid (NMDA)-induced Excitotoxicity. n -甲基- d -天冬氨酸（NMDA）诱导的兴奋性毒性对视网膜神经节细胞解剖特征的影响。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-19 DOI: 10.3791/68537

Takae Kiyama, Ashlyn Tu, Chai-An Mao

{"title":"Examination of Anatomical Features of Retinal Ganglion Cells Under N-methyl-D-aspartic Acid (NMDA)-induced Excitotoxicity.","authors":"Takae Kiyama, Ashlyn Tu, Chai-An Mao","doi":"10.3791/68537","DOIUrl":"https://doi.org/10.3791/68537","url":null,"abstract":"Retinal ganglion cells (RGCs) are the output neurons of the retina, responsible for transmitting visual information to the brain. In the mature mouse retina, over 40 RGC types have been identified based on genomic, morphological, and functional characteristics. RGC degeneration is a hallmark of various debilitating retinal diseases, including glaucoma and traumatic optic neuropathy. Gaining deeper insight into the cellular and molecular mechanisms underlying RGC degeneration, survival, and resilience across different disease conditions is crucial for developing effective therapeutic interventions. To investigate RGC degeneration, we adopted a simple yet powerful technique previously used extensively in retinal development studies for RGC morphologies and central projection. Here, we apply this method to assess the anatomical phenotypes of RGCs under NMDA-induced excitotoxicity. This approach enables the visualization of large numbers of degenerating RGCs over time, allowing us to map the sequence of degenerative events, identify hallmark phenotypes of dying RGCs, and distinguish RGCs that are resistant to NMDA-induced damage.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145240069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the Effect of SASP Factors on the Proliferation of Cancer Cells using A Comparative Analysis of Three Distinct Methodologies. 用三种不同方法比较分析SASP因子对癌细胞增殖的影响。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-19 DOI: 10.3791/68883

Yaprak Dilber Şimay Demir, Tuğçe Tayyar, Aysun Özdemir, Mustafa Ark

{"title":"Evaluating the Effect of SASP Factors on the Proliferation of Cancer Cells using A Comparative Analysis of Three Distinct Methodologies.","authors":"Yaprak Dilber Şimay Demir, Tuğçe Tayyar, Aysun Özdemir, Mustafa Ark","doi":"10.3791/68883","DOIUrl":"https://doi.org/10.3791/68883","url":null,"abstract":"Chemotherapy-induced senescent cancer cells also secrete various factors, called senescence-associated secretory phenotype, to regulate their extracellular microenvironment. Previous studies have reported that these SASP factors exert detrimental paracrine effects on surrounding cells, including promoting proliferation, epithelial-mesenchymal transition (EMT), angiogenesis, and migration. Several in vitro co-culture techniques are widely used to understand the cellular processes resulting from interaction by culturing the same and different types of cells together. Here, the potential proliferative effects of SASP factors secreted by senescent HeLa cells on non-senescent HeLa cells were investigated using three complementary in vitro approaches. The first approach involved co-culturing senescent and non-senescent cells to investigate the paracrine signaling mediated by the SASP. In the second approach, conditioned media collected from senescent cancer cells were concentrated and subsequently used to examine the impact of the conditioned media with real-time monitoring of proliferation. In the third method, senescent and non-senescent cells were cultured side by side to assess the juxtacrine effects of SASP through direct cell-to-cell contact. All three experimental models consistently demonstrated that SASP factors significantly enhanced the proliferation of non-senescent cancer cells. Notably, senescent cell co-culture increased the proliferation rate by 64.6%, and 3x concentrated SASP-conditioned media increased proliferation by over 50% compared to controls. Fluorescence-based imaging showed a 49.3% increase in GFP-positive cell numbers under juxtacrine conditions. These methods collectively enabled quantitative and qualitative evaluation of SASP-induced proliferative changes. The comparative analysis of these approaches highlights their respective strengths and limitations, providing valuable insights into the paracrine effects of senescent cells.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145240085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Subcutaneous Mastectomy under Laparoscopy with In Situ Prosthetic Reconstruction on Aesthetic Appearance in Breast Cancer Patients. 腹腔镜下皮下乳房切除术与原位假体重建对乳腺癌患者美观的影响。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-19 DOI: 10.3791/68297

Guojian Shi, Lili Hou, Lei Yin, Shuang Hou, Shuai Zhao, Xiaohua Li

{"title":"Impact of Subcutaneous Mastectomy under Laparoscopy with In Situ Prosthetic Reconstruction on Aesthetic Appearance in Breast Cancer Patients.","authors":"Guojian Shi, Lili Hou, Lei Yin, Shuang Hou, Shuai Zhao, Xiaohua Li","doi":"10.3791/68297","DOIUrl":"https://doi.org/10.3791/68297","url":null,"abstract":"Breast cancer remains one of the most widespread malignant tumors affecting women, with a range of treatment options available, among which surgical intervention holds a central position. Traditional mastectomy, while effective in removing tumors, often results in severe damage to the breast's appearance, triggering significant psychological stress and emotional distress for patients, which in turn may affect their post-treatment quality of life and recovery process. Thanks to advancements in medical technology, a novel approach-laparoscopic subcutaneous mastectomy combined with in situ prosthetic breast reconstruction-has emerged. This innovative surgical method not only ensures thorough tumor resection but also maximizes the preservation of the breast's natural structure and contour, thereby significantly improving the overall aesthetic outcome for breast cancer patients. This study aims to explore the specific impact of this surgical approach on the overall aesthetic appearance of breast cancer patients. It will detail critical elements such as patient selection criteria, comprehensive preoperative preparation, meticulous surgical approach planning, step-by-step surgical procedures, and comparative analyses of postoperative breast appearance. Through this exploration, the study seeks to offer practical references and guidance for clinical practice.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145240095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retrospective Case Analysis of Transnasal Endoscopic Resection of Orbital Apex Tumor. 经鼻内镜眶尖瘤切除术回顾性病例分析。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-16 DOI: 10.3791/68875

Hui Qi, Cheng Xiong, Qinglan Xiong, Guiqin Liu, Xiang Tu

{"title":"Retrospective Case Analysis of Transnasal Endoscopic Resection of Orbital Apex Tumor.","authors":"Hui Qi, Cheng Xiong, Qinglan Xiong, Guiqin Liu, Xiang Tu","doi":"10.3791/68875","DOIUrl":"https://doi.org/10.3791/68875","url":null,"abstract":"Endoscopic endonasal surgery has gained increasing recognition as an effective approach for the management of orbital tumors. In this study, we retrospectively analyzed the clinical data of eight patients with orbital apex tumors (OATs) who underwent endoscopic endonasal resection at the Orbital Disease and Ophthalmic Oncology Department of Shenzhen Eye Hospital between April 2021 and September 2024. The cohort consisted of one male and seven female patients, with a mean age of 41.7 ± 14.2 years. All patients successfully underwent complete tumor resection by the endoscopic endonasal approach, followed by standardized postoperative treatment including anti-inflammatory therapy, corticosteroids, and neurotrophic medications. Histopathological examination revealed five cases of cavernous hemangioma (62.5%), two cases of schwannoma (25%), and one case of metastatic carcinoma (12.5%). During the follow-up period of at least 6 months, visual acuity improved in five patients (62.5%), decreased in two patients (25%), and remained unchanged in one patient (12.5%). No postoperative complications were observed, including ocular motility disorders, visual field defects, cerebrospinal fluid leakage, or hemorrhage. All patients with benign tumors showed no evidence of recurrence, while the patient with a malignant tumor received adjuvant radiotherapy. Endoscopic endonasal resection represents a safe, minimally invasive, and effective surgical option for selected OATs. The procedure demonstrates significant advantages in terms of clinical outcomes and complication avoidance. However, careful patient selection based on tumor characteristics and precise surgical planning remain crucial for optimal results. These findings support the clinical application of this technique for the management of orbital apex tumors.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145240154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Post-Liver Transplantation Management and Specimen Collection in Daurian Ground Squirrels for Single-Cell RNA Sequencing. 达斡尔地松鼠肝移植后的处理和单细胞RNA测序的标本收集。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-16 DOI: 10.3791/68443

Haobin Sun, Yuqing Qiao, Zipei Wang, Bo Yang, Xiaoling Ren, Ming Yang, Shiqiang Wang, Jingxing Ou, Xin Xing

{"title":"Post-Liver Transplantation Management and Specimen Collection in Daurian Ground Squirrels for Single-Cell RNA Sequencing.","authors":"Haobin Sun, Yuqing Qiao, Zipei Wang, Bo Yang, Xiaoling Ren, Ming Yang, Shiqiang Wang, Jingxing Ou, Xin Xing","doi":"10.3791/68443","DOIUrl":"https://doi.org/10.3791/68443","url":null,"abstract":"Graft functional recovery and graft-host immunocompatibility represent major prognostic concerns following liver transplantation. Critically, conventional rodent models using rats or mice offer limited insight into the mechanisms of hypothermia-induced graft injuries during preservation. This gap underscores the need for alternative models. Since liver grafts from the cold-adaptive Daurian ground squirrel (DGS; Spermophilus dauricus) can sustain prolonged cold preservation and hence support high survivability in post-transplantation DGSs, consequently, this protocol details the essential post-surgical animal care and physiological monitoring required for transplanted DGS subjects. Furthermore, it provides standardized procedures for preparing high-quality liver graft samples for downstream analytical techniques, including single-cell RNA sequencing (scRNA-seq) for transcriptomic profiling and histopathological examination. Successfully generating well-prepared DGS graft specimens is the crucial initial step in applying both traditional and cutting-edge methodologies to study this unconventional yet highly relevant model. This protocol addresses the multi-omic information for DGS, thereby facilitating future research on surgical interventions and disease modeling not only in hibernating rodents but also in other specialized organisms.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145240168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage. 单细胞质谱代谢组学研究的样品制备：联合细胞洗涤，淬火，干燥和储存。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-16 DOI: 10.3791/68995

Deepti Bhusal, Shakya Wije Munige, Zongkai Peng, Dan Chen, Zhibo Yang

{"title":"Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage.","authors":"Deepti Bhusal, Shakya Wije Munige, Zongkai Peng, Dan Chen, Zhibo Yang","doi":"10.3791/68995","DOIUrl":"https://doi.org/10.3791/68995","url":null,"abstract":"Single-cell mass spectrometry (SCMS) has become an indispensable tool for studying cellular metabolism. Owing to advancements in modern mass spectrometry (MS) techniques and demand in studies of cell heterogeneity in fundamental biological sciences and human diseases, a variety of different SCMS techniques have been developed and applied in laboratory research. As metabolites can accurately reflect cell status, SCMS metabolomics analysis of live cells is regarded as a powerful tool to provide molecular information about cells. However, a major challenge in SCMS analysis of live cells is preserving the endogenous metabolite profiles during sample preparation, transport, and measurement. Cellular metabolites undergo rapid turnover and are highly sensitive to environmental changes, making them susceptible to degradation or transformation prior to analysis. To address this limitation, we present a robust and reproducible cell preparation protocol designed to preserve cellular metabolite integrity for SCMS. The protocol integrates cell washing with a volatile ammonium formate (AF) solution, rapid quenching with liquid nitrogen (LN2), vacuum freeze-drying, and storage at -80 °C. This approach minimizes cell membrane damage while effectively halting metabolic activity. The results indicate that rapid cell quenching is vital; however, limiting storage time at -80 °C is necessary to preserve cell metabolites. The proposed protocol can potentially be used for other existing SCMS techniques for broad applications.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145240191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adjuvant Ayurvedic Management in Coronary Artery Disease with Coronary Microvascular Dysfunction. 阿育吠陀辅助治疗冠心病伴冠状动脉微血管功能障碍。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-16 DOI: 10.3791/68399

Satyajit Pandurang Kulkarni

{"title":"Adjuvant Ayurvedic Management in Coronary Artery Disease with Coronary Microvascular Dysfunction.","authors":"Satyajit Pandurang Kulkarni","doi":"10.3791/68399","DOIUrl":"https://doi.org/10.3791/68399","url":null,"abstract":"This case study presents a 59-year-old obese, hypertensive female with a 10-year history of Coronary Microvascular Dysfunction and Coronary Artery Disease, presenting with associated symptoms including mild to moderate body aches, pain in the left ankle, and insomnia. Diagnosed with Vataj Hridroga according to Ayurvedic principles, the patient underwent a 14-day inpatient Ayurvedic treatment regimen, followed by 16 weeks of outpatient oral Ayurvedic medications, alongside her existing modern medical prescriptions. The inpatient regimen included daily Panchakarma therapies: Abhyanga and Nadi Swedana for pain and edema; Shirodhara was administered for insomnia. Oral medications, including Simhanada Guggulu, Ajmodadi Churna, Dashmoola Kwatha, and Bramhi Vati, were prescribed. The integrative strategy involved close monitoring by both Ayurvedic and modern medicine physicians, allowing for necessary adjustments to contemporary medication. Significant improvements in the symptoms were observed: leg pain reduced from a baseline score of 6 to 1 by day 16 and resolved by week 16; grade 2 edema resolved by day 16; and insomnia improved, evidenced by a reduction in Pittsburgh Sleep Quality Index score from 16/21 at baseline to 9/21 by both day 16 and week 26. This report highlights the adjunctive role of Ayurvedic interventions in managing associated symptoms in a complex patient with CMVD and CAD, along with relevant risk factors. This protocol emphasizes the strategic integration of Ayurvedic Panchakarma and oral treatments with contemporary medical therapies. Further rigorous research is warranted to elucidate the mechanisms of action and assess the effectiveness of such integrative approaches combining Ayurveda with conventional medicine, particularly in cardiac disorders.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145240063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Impact of CYP3A4, COMT V158M, and OPRM A118G Gene Polymorphisms on the Effectiveness of Sufentanil in Labor Analgesia. CYP3A4、COMT V158M、OPRM A118G基因多态性对舒芬太尼分娩镇痛效果的影响

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-16 DOI: 10.3791/69160

Huiqiong Huang, Yunhong Zhang, Junxiang Jia, Zhuanghui Zhu

{"title":"The Impact of CYP3A4, COMT V158M, and OPRM A118G Gene Polymorphisms on the Effectiveness of Sufentanil in Labor Analgesia.","authors":"Huiqiong Huang, Yunhong Zhang, Junxiang Jia, Zhuanghui Zhu","doi":"10.3791/69160","DOIUrl":"https://doi.org/10.3791/69160","url":null,"abstract":"Genetic polymorphisms in CYP3A4, COMT V158M, and OPRM A118G may influence analgesic response, hemodynamic regulation, and postpartum recovery, while parity is thought to affect labor outcomes and adverse events. This study investigated how these genotypes and parity impact maternal and neonatal outcomes, labor duration, pain control, and postpartum complications in 380 pregnant women stratified by genotype. Data on baseline characteristics, labor duration, VAS pain scores, and postpartum adverse events were collected, and safety indicators such as blood pressure, heart rate, fetal outcomes, and Apgar scores were analyzed. Associations between parity and postpartum responses were explored using univariate and logistic regression models. Baseline variables were largely comparable across genotypes except for a higher BMI in the CYP3A4 CT/TT group. Sufentanil consumption was significantly higher in CYP3A4 CT/TT and OPRM A118G GA/GG carriers, although labor duration did not differ among genotypes. OPRM A118G GA/GG carriers also showed a higher incidence of fever (P = 0.016) and greater reductions in blood pressure after intervention (P < 0.001), though all groups achieved similar pain relief. Multiparas experienced less urinary retention (P = 0.005) but more pruritus (P = 0.011), and logistic regression confirmed smaller decreases in systolic blood pressure (OR = 1.049, P = 0.001) and lower risk of pruritus (OR = 0.326, P = 0.01) in this group. Although CYP3A4, COMT, and OPRM A118G genotypes did not broadly influence maternal or neonatal outcomes, carriers of CYP3A4 CT/TT and OPRM A118G GA/GG required higher sufentanil doses and displayed genotype-specific hemodynamic responses, while parity was associated with distinct adverse event profiles and blood pressure changes. These findings highlight the relevance of genetic polymorphisms and parity in guiding personalized postpartum care.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145240132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Agreement and Accuracy using Binocular Wavefront Optometer with Autorefractor and Phoropter. 带自折射镜的双目波前视光计与光镜的一致性和精度比较。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-16 DOI: 10.3791/68422

Suqi Xian, Yifan Tang, Peng Ji, Hongsheng Bi, Qingbao Wang, Xiaofan Wang, Li Li

{"title":"Comparison of Agreement and Accuracy using Binocular Wavefront Optometer with Autorefractor and Phoropter.","authors":"Suqi Xian, Yifan Tang, Peng Ji, Hongsheng Bi, Qingbao Wang, Xiaofan Wang, Li Li","doi":"10.3791/68422","DOIUrl":"https://doi.org/10.3791/68422","url":null,"abstract":"The aim is to evaluate the agreement and accuracy of refraction using a binocular wavefront optometer with the autorefractor and phoropter. To do this, 1 month after corneal refractive surgery, the right eyes of 125 subjects were enrolled in this cross-sectional study. Objective and subjective refractions were measured using a binocular wavefront optometer, an autorefractor, and a phoropter in a noncycloplegic condition. The differences and agreement of refractive data were analyzed using the Wilcoxon signed-rank test and Bland-Altman plots. The differences between sphere (Z=-0.872, p=0.38), J0 (Z=-0.065, p=0.95), J45 (Z=-1.199, p=0.23), and SE (Z=-1.316, p=0.19), objectively detected by binocular wavefront optometer and autorefractor, were not statistically significant. The differences between J0 (Z=-0.533, p=0.59), J45 (Z=-0.724, p=0.47), and SE (Z=-0.933, p=0.35), subjectively detected by binocular wavefront optometer and phoropter, were not statistically significant, whereas sphere (Z=-3.699, p<0.0001) had statistically significant differences. The Bland-Altman plots showed agreement between binocular wavefront optometer objective refraction and autorefractor in sphere (95% LoA: -1.00 D to 0.89 D), J0 (95% LoA: -0.47 D to 0.47 D), J45 (95% LoA: -0.55 D to 0.58 D), and SE (95% LoA: -0.89 D to 0.96 D). The Bland-Altman plots also showed agreement between binocular wavefront optometer subjective refraction and phoropter in sphere (95% LoA: -0.59 D to 0.82 D), J0 (95% LoA: -0.47 D to 0.43 D), J45 (95% LoA: -0.47 D to 0.43 D), and SE (95% LoA: -0.66 D to 0.74 D). Conclusively, the binocular wavefront optometer is an effective instrument, demonstrating good agreement between its objective and subjective refraction results and those obtained from autorefractor and phoropter, its smaller refractive intervals presenting a high accuracy in refractive error detection.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145240027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating Ischemic Stroke Recurrent Injury in a Secondary Middle Cerebral Artery Occlusion Model in Mouse. 小鼠继发性大脑中动脉闭塞模型缺血性卒中复发性损伤的研究。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-09-16 DOI: 10.3791/68806

Yi-Je Chen, Cheng-Tien Wu, Chang-Mu Chen, Shing-Hwa Liu

{"title":"Investigating Ischemic Stroke Recurrent Injury in a Secondary Middle Cerebral Artery Occlusion Model in Mouse.","authors":"Yi-Je Chen, Cheng-Tien Wu, Chang-Mu Chen, Shing-Hwa Liu","doi":"10.3791/68806","DOIUrl":"https://doi.org/10.3791/68806","url":null,"abstract":"Stroke is the second leading global cause of mortality and disability, with recurrent strokes contributing significantly to poor outcomes and increased neurological burden. Despite advances in acute clinical stroke management, experimental models for recurrent ischemic stroke remain limited due to their procedural complexity and high mortality rates. In this study, we aimed to establish a recurrent ischemic stroke in a mouse model and evaluate the brain pathological changes and functional deficits using a two-stage middle cerebral artery occlusion (MCAO) protocol. Adult male C57BL/6J mice underwent transient MCAO under Laser Doppler blood flow monitoring. A secondary MCAO was performed 14 days after the initial surgery to simulate stroke recurrence. Postoperative assessments included locomotor activity analysis, neurobehavioral deficits scoring by using a six-point scoring system, inflammatory markers including hypoxia-inducible factor-1α (HIF-1α) and tumor necrosis factor-α (TNF-α), and histopathological evaluation using hematoxylin and eosin (H&E) staining. After recurrent MCAO administration, mice exhibited significantly reduced locomotor activity, decreased movement speed, and diminished neurobehavioral scores, as well as increased inflammatory markers, compared to control groups. Histopathological detection revealed moderate to severe neuronal cell necrosis, neuropil vacuolation, and localized hemorrhage in the cortex and hippocampus of recurrent MCAO mice. The inflammatory markers were also induced in this recurrent MCAO procedure. This two-stage MCAO mouse model presents, for the first time, a method that effectively simulates recurrent ischemic stroke with consistent neurological and pathological outcomes and improves postoperative survival. This model could provide a reliable platform for mechanistic studies and may be applied in the future evaluation of neuroprotective therapies for recurrent stroke.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 223","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145240025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0