Jove-Journal of Visualized Experiments最新文献_第6页

Orthotopic Left Lung Transplantation in Rats. 大鼠原位左肺移植。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-07-03 DOI: 10.3791/68106

Hailin Liao, Xiaohua Wang, Yi Lu, Wenshan Zhong, Jiang Shi, Xihui Huang, Xu Chen, Guilin Li, Penghui Yang, Chunrong Ju

{"title":"Orthotopic Left Lung Transplantation in Rats.","authors":"Hailin Liao, Xiaohua Wang, Yi Lu, Wenshan Zhong, Jiang Shi, Xihui Huang, Xu Chen, Guilin Li, Penghui Yang, Chunrong Ju","doi":"10.3791/68106","DOIUrl":"https://doi.org/10.3791/68106","url":null,"abstract":"The rat left lung orthotopic transplantation model is an essential tool in lung transplant research. Although lung transplantation is a complex procedure, its complexity is significantly reduced with the introduction of the cuff technique. Most surgeons can master this technique after training, but its application has been limited due to the long learning curve and low model survival rate. To address these issues, we propose an improved technique with high repeatability, which includes donor lung harvest, modification, and implantation. Compared to previous methods, our improvements primarily focus on the dissection of the donor lung harvest, donor lung modification, and donor lung implantation. This modification simplifies the procedure, reduces operational difficulty, and increases repeatability. Additionally, the cold ischemia and warm ischemia times during the procedure have been shortened, further improving the success rate of transplantation. Our research provides a more efficient and reproducible technical foundation for the rat lung transplantation model, supporting future research in lung transplantation.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 221","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cancer-Associated Fibroblasts from Mouse Mammary Tumors as Tools for Molecular and Computational Studies. 小鼠乳腺肿瘤肿瘤相关成纤维细胞作为分子和计算研究的工具。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-07-03 DOI: 10.3791/68364

Daniele Viavattene, Aurora Savino, Somayeh Mirzaaghaei, Andrea Lobascio, Valeria Poli, Lidia Avalle

{"title":"Cancer-Associated Fibroblasts from Mouse Mammary Tumors as Tools for Molecular and Computational Studies.","authors":"Daniele Viavattene, Aurora Savino, Somayeh Mirzaaghaei, Andrea Lobascio, Valeria Poli, Lidia Avalle","doi":"10.3791/68364","DOIUrl":"https://doi.org/10.3791/68364","url":null,"abstract":"A compelling body of research has confirmed the critical role of the tumor microenvironment (TME) in the growth and aggressiveness of breast cancer (BC). Cancer-associated fibroblasts (CAF), the predominant cell component of the TME, significantly influences its behavior through complex crosstalk with cancer and other stromal cells in response to tumor-derived activating signals. Murine models are extensively used to investigate the mechanisms underlying CAF's role in BC progression, as they allow for the evaluation of the endogenous TME and the assessment of immune system involvement using syngeneic xenograft studies. Here, we present a set of methods for culturing BC-derived CAFs, manipulating gene expression, and evaluating their pro-tumorigenic functions using both in vitro and in vivo approaches. We also describe protocols for CAF gene expression profiling and transcriptomic analysis, alongside a computational procedure for inferring transcriptional signatures in primary tumors using publicly available bulk RNA-seq data. Additionally, we introduce a user-friendly, web-based tool for analyzing the transcriptomes of stromal and epithelial components from laser capture micro-dissected BC tumors. Ultimately, these methods can provide valuable insights into the molecular mechanisms driving CAF-tumor interactions and hold potential for identifying novel therapeutic targets.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 221","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental Sampling of Photosynthetic Microbes and Their Viruses: From Field to Lab. 光合微生物及其病毒的环境采样：从野外到实验室。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-07-03 DOI: 10.3791/68379

Sobroney Heng, Ruben Michael Ceballos

{"title":"Environmental Sampling of Photosynthetic Microbes and Their Viruses: From Field to Lab.","authors":"Sobroney Heng, Ruben Michael Ceballos","doi":"10.3791/68379","DOIUrl":"https://doi.org/10.3791/68379","url":null,"abstract":"Environmental sampling of photosynthetic microorganisms and their viruses plays a critical role in understanding contemporary marine and freshwater biodiversity and ecosystem dynamics as well as the impacts of climate change-related factors (e.g., rising temperatures and acidification) on evolutionary trajectories of species and community composition. Unfortunately, the diversity of the virosphere does not support a single universal sampling and experimental workflow. Indeed, each virus system has unique features, which require modifications to standard protocols in virology to accomplish research goals. Although virus discovery and characterization require approaches that are specific to the target system, for all viruses, the research aims are similar: isolate the virus; determine host range; confirm productive infection; and characterize the virus, the host, and virus-host dynamics. Robust descriptions of virus-host systems consist minimally of elucidating morphology, physiology, biochemistry, and omics profiles. Further information may be obtained by manipulating the system by changing factors such as multiplicity of infection, temperature, pH, host-switch, directed evolution, or applying drugs to observe virus-host system response. Our laboratory studies viruses across domains of life (Archaea, Bacteria, and Eukarya). In this report, we detail methods for sampling photosynthetic microbes from the euphotic zone of freshwater and marine environments with focus on isolating bacteriophage (i.e., cyanophage) of cyanobacteria. Cyanobacteria are keystone species critical to primary production and nutrient cycling in these aquatic ecosystems. The described workflow extends from sampling waters at different depths to characterizing virus-host system features using liquid and solid media culture, advanced molecular/genetic methods, and analytical approaches. The methods described are adaptable to bacteriophage and virus discovery in virus-host systems across domains of life.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 221","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of Compound Giant Unilamellar Vesicles: A Biomimetic Model of Nucleate Cells. 复合巨型单层囊泡的合成：有核细胞的仿生模型。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-07-03 DOI: 10.3791/68274

Rupesh Kumar, Rajarshi Chakrabarti, Rochish M Thaokar

{"title":"Synthesis of Compound Giant Unilamellar Vesicles: A Biomimetic Model of Nucleate Cells.","authors":"Rupesh Kumar, Rajarshi Chakrabarti, Rochish M Thaokar","doi":"10.3791/68274","DOIUrl":"https://doi.org/10.3791/68274","url":null,"abstract":"A simple Giant unilamellar vesicle (sGUV) is a micron-sized spherical vesicle, which is composed of a lipid bilayer that encloses an aqueous solution inside and is suspended in another aqueous medium. As biomimetic models of biological cells, sGUVs are well-established systems for biophysical studies. However, sGUVs primarily mimic anucleate cells, limiting their application as models for non-nucleate cells. We propose compound Giant Unilamellar vesicles (cGUVs) as a more appropriate biomimetic model for nucleated cells. cGUVs are vesicle-in-vesicle structures, where the outer and inner vesicle bilayers can be assumed to represent the cell and the nuclear membrane of a cell, respectively. In this study, we describe a simple method for the synthesis of cGUVs. Briefly, sGUVs were generated using the lipid composition of DMPC and cholesterol within the range of 37-43 mol% in a sucrose medium via the electroformation method. These sGUVs were then subjected to osmotic shock by introducing a hypertonic glucose solution, triggering an immediate transition to a somatocytic shape. This process led to the formation of an intermediate state where the outer and inner vesicles remained connected through a neck, ultimately resulting in the development of vesicle-in-vesicle structure, referred to as cGUVs. Furthermore, it is known that GUVs prepared with higher cholesterol levels in the bilayer exhibit reduced ion permeability. This characteristic allows tuning of conductivity in the annular solution of a cGUV by adjusting the conductivities of the hydrating medium, the inner solution through hypertonic glucose, and the outer solution by adjusting of the dilution or the addition of conductive media, making the resultant cGUVs with controlled conductivities in the outer, annular, and inner regions, relevant for electric field studies. We propose this efficient and straightforward approach for synthesizing compound vesicles that can be used as a biomimetic model for eukaryotic, nucleated cells, advancing their application in biophysical research.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 221","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of Mitochondrial Morphology in Live Cells Using Confocal Microscopy. 用共聚焦显微镜测定活细胞线粒体形态。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-07-03 DOI: 10.3791/68167

Manna Lin, Jiakang Wang, Zihong Xian, Chunyuan Zeng, Jiangping Xu

{"title":"Determination of Mitochondrial Morphology in Live Cells Using Confocal Microscopy.","authors":"Manna Lin, Jiakang Wang, Zihong Xian, Chunyuan Zeng, Jiangping Xu","doi":"10.3791/68167","DOIUrl":"https://doi.org/10.3791/68167","url":null,"abstract":"The dynamic balance of mitochondrial fusion and fission directly contributes to mitochondrial homeostasis, which influences numerous cellular functions in addition to adenosine triphosphate (ATP) homeostasis. Therefore, assessing mitochondrial morphology under stress conditions is essential for mechanistic research. This study describes a detailed protocol for analyzing mitochondrial morphology, encompassing the preparation of a MitoTracker solution, staining of mitochondria, optimization of imaging parameters, and detection of morphological features. MitoTrackers are commonly used, cost-effective mitochondrion-specific dyes. However, some changes in mitochondrial morphology may occur owing to inappropriate handling, which can be unperceivable and fail to reflect the true state of mitochondria. Therefore, it is necessary to understand how to analyze changes in mitochondrial morphology using MitoTrackers. The protocol utilized SH-SY5Y cells stimulated with 1-methyl-4-phenylpyridinium iodide (MPP+) to illustrate the protocol of mitochondrial morphological analysis. Compared with control cells, MPP+-stimulated cells exhibited smaller and more fragmented mitochondria, with morphological parameters indicating decreased mitochondrial footprint. These results suggest that MitoTracker staining is an effective and feasible method for mitochondrial morphological analysis that (with minor modifications) can be applied to study various conditions.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 221","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Measurement of Cyclic Guanosine Monophosphate (cGMP) in Solid Tissues using Competitive Enzyme-Linked Immunosorbent Assay (ELISA). 竞争酶联免疫吸附试验（ELISA）测定固体组织中环鸟苷单磷酸（cGMP）的含量。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-07-03 DOI: 10.3791/68432

Haleigh A Brown, Barbora Piknova, Ji Won Park, Alan N Schechter

{"title":"Measurement of Cyclic Guanosine Monophosphate (cGMP) in Solid Tissues using Competitive Enzyme-Linked Immunosorbent Assay (ELISA).","authors":"Haleigh A Brown, Barbora Piknova, Ji Won Park, Alan N Schechter","doi":"10.3791/68432","DOIUrl":"https://doi.org/10.3791/68432","url":null,"abstract":"A major pathway that produces cyclic guanosine monophosphate (cGMP) is the nitric oxide (NO)-cGMP pathway. In this pathway, NO stimulates the catalytic activity of soluble guanylyl cyclase (sGC), which converts guanosine triphosphate to cGMP. NO is of interest because, in mammals, it acts as the main vasodilator, an inhibitor of platelet aggregation, a mediator of immune function, a neurotransmitter, and to improve endurance during physical activity, among other physiological roles. Measuring NO's abundance in different tissues could help elucidate its functions. However, NO cannot be measured directly due to its less than seconds-long half-life. Typically, NO's metabolites, nitrate and nitrite, are measured to estimate NO's abundance. This is because these molecules can be interconverted in vivo through redox reactions. A less common method of estimating NO's abundance requires measuring the concentration of cGMP, a signaling molecule downstream of NO. Yet, measuring cGMP in tissues is seldom done, despite creating the possibility to better understand NO's functions in organs. Therefore, a need exists for a protocol to measure cGMP concentrations in solid organs and tissues. In this protocol, collected pig tissues were homogenized, competitive ELISA was performed on processed samples, and absorbances were converted into nanomoles of cGMP per gram of original tissue. In short, this protocol delineates a simplistic method to measure cGMP concentrations in solid tissues using the cyclic GMP ELISA kit.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 221","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pulmonary Embolism In-Transit Crossing the Mitral and Aortic Valve. 途经二尖瓣和主动脉瓣的肺栓塞。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-07-03 DOI: 10.3791/67682

Kirsten Freeman, Lindsey Brinkley, Ryan Azarrafiy, Todd E Jones, Thomas Beaver

{"title":"Pulmonary Embolism In-Transit Crossing the Mitral and Aortic Valve.","authors":"Kirsten Freeman, Lindsey Brinkley, Ryan Azarrafiy, Todd E Jones, Thomas Beaver","doi":"10.3791/67682","DOIUrl":"https://doi.org/10.3791/67682","url":null,"abstract":"Hemodynamically significant pulmonary embolisms (PE) in the pulmonary artery or those crossing a patent foramen ovale (PFO) can become life-threatening emergencies requiring immediate removal. While cases of surgical management for a PE crossing a PFO have been reported, there are no documented cases of a PE extending across both the mitral and aortic valves. A 38-year-old female with a medical history of obesity, depression, and thalassemia minor was transferred from an outside hospital with hemodynamic compromise due to a large PE. Transesophageal echocardiography (TEE) revealed a PE crossing the PFO, extending across the mitral valve, and traversing the aortic valve into the ascending aorta. Cardiopulmonary bypass (CPB) was initiated using standard aortic cannulation distal to the known clot, along with bicaval venous cannulation. The heart was arrested, and the right and left atria were opened in a transeptal fashion. The clot, which extended from the PFO through the mitral and aortic valves, was identified and removed en bloc. A small aortotomy was performed, confirming a clear aorta. An additional clot was found in the right atrium crossing the tricuspid valve and was subsequently removed. Finally, the pulmonary artery was opened, and the bulk of the clot burden was extracted, completing the embolectomy. The patient was successfully weaned from CPB without complications and was extubated within 24 h post-surgery. She underwent inferior vena cava filter placement and was discharged on post-operative day seven. Extensive pulmonary emboli are life-threatening emergencies that require immediate intervention. This study presents an extensive clot burden crossing the PFO, mitral valve, and aortic valve. Prompt surgical intervention is crucial to preventing severe complications such as stroke, myocardial infarction, respiratory failure, death, and long-term sequelae, including pulmonary hypertension and future strokes.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 221","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liver Cold Storage and Transplantation in the Cold-Adaptive Daurian Ground Squirrels. 冷适应性达斡尔地松鼠肝脏的冷藏和移植。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-07-03 DOI: 10.3791/68444

Zipei Wang, Haobin Sun, Xin Xing, Yuqing Qiao, Xiaoling Ren, Jingxing Ou, Zhishui Chen, Bo Yang

{"title":"Liver Cold Storage and Transplantation in the Cold-Adaptive Daurian Ground Squirrels.","authors":"Zipei Wang, Haobin Sun, Xin Xing, Yuqing Qiao, Xiaoling Ren, Jingxing Ou, Zhishui Chen, Bo Yang","doi":"10.3791/68444","DOIUrl":"https://doi.org/10.3791/68444","url":null,"abstract":"In liver transplantation, the functional recovery of donor grafts following extended cold storage is a major challenge. The same obstacle applies to standard rodent models (e.g., mice and rats) for liver transplantation despite their well-established surgical protocols, thus restricting research on key topics such as post-transplantation functional recovery and host-graft immunocompatibility. Here, we successfully established a novel liver transplantation model using an obligate hibernator, the Daurian ground squirrel (DGS; Spermophilus dauricus), which demonstrates intrinsic adaptation to ultra profound hypothermia-rewarming and ischemia-reperfusion transitions. In our hands, transplantation of liver grafts following 24-h cold storage resulted in 100% survival of DGS recipients within the 7-day observation time frame, whilst the same procedure in Sprague-Dawley (SD) rats had 0% survival 24 h following the surgery. Thus, the DGS and other cold-adaptive mammalian models provide a unique and robust platform to investigate mechanisms underlying organ cold adaptation and transplantation tolerance, which can support the development of novel treatment or surgery strategies in the field of transplantation medicine.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 221","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-omics Techniques for Profiling Chromatin State Transitions in Low-input Primary Mouse Cholangiocytes. 分析小鼠低输入原代胆管细胞染色质状态转变的多组学技术。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-07-03 DOI: 10.3791/68606

Rongjie Ji, Jiayuan Chen, Bo Qing, Xinyi Cao, Zhigang Zhao, Zhiheng Liu

{"title":"Multi-omics Techniques for Profiling Chromatin State Transitions in Low-input Primary Mouse Cholangiocytes.","authors":"Rongjie Ji, Jiayuan Chen, Bo Qing, Xinyi Cao, Zhigang Zhao, Zhiheng Liu","doi":"10.3791/68606","DOIUrl":"https://doi.org/10.3791/68606","url":null,"abstract":"Polycystic liver disease (PLD) is a hereditary disorder characterized by the formation of fluid-filled cysts derived from cholangiocytes, leading to progressive disease and a significant reduction in patients' quality of life. Current treatments for PLD are inadequate, emphasizing the need for novel therapeutic strategies. The role of epigenetic regulation in PLD progression, particularly chromatin accessibility and histone modifications, remains underexplored. Traditional epigenetic profiling techniques, such as ChIP-seq and DNase-seq, require large numbers of cells, which are difficult to obtain from primary cholangiocytes. To address this, we optimized low-input ChIP-seq and ATAC-seq protocols for low numbers of primary cholangiocytes. These approaches allow for the analysis of histone modifications and chromatin accessibility with minimal cell input. Low-input ChIP-seq utilizes micrococcal nuclease (MNase) for DNA fragmentation, while ATAC-seq employs Tn5 transposase to capture open chromatin regions. These multi-omics techniques provide valuable insights into chromatin state dynamics during cholangiocyte fate transitions in PLD and other biliary diseases. Importantly, the optimized protocols are confidently applicable to other low-input primary cells, enabling the exploration of epigenetic mechanisms across various cellular contexts. This work presents a systematic approach for studying chromatin state alterations, contributing to the development of epigenetic-based therapeutic strategies for PLD and related diseases.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 221","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Point-of-care Ultrasound to Screen for Gastrointestinal Dysfunction: Image Acquisition and Interpretation. 点护理超声筛查胃肠道功能障碍：图像采集和解释。

IF 1.2 4区综合性期刊

Jove-Journal of Visualized Experiments Pub Date : 2025-07-03 DOI: 10.3791/68603

Lior Abramson, Jacqueline K Olive, Brice Lefler, Lindsey Wu, Amber Bowman, Tyler Simpson, Cory Vatsaas, Mamata Yanamadala, Yuriy S Bronshteyn

{"title":"Point-of-care Ultrasound to Screen for Gastrointestinal Dysfunction: Image Acquisition and Interpretation.","authors":"Lior Abramson, Jacqueline K Olive, Brice Lefler, Lindsey Wu, Amber Bowman, Tyler Simpson, Cory Vatsaas, Mamata Yanamadala, Yuriy S Bronshteyn","doi":"10.3791/68603","DOIUrl":"https://doi.org/10.3791/68603","url":null,"abstract":"A major cause of morbidity and healthcare resource utilization is gastrointestinal (GI) dysfunction. Although GI symptoms are frequently evaluated with computed tomography (CT), this modality subjects patients to ionizing radiation, high costs, and high resource utilization (including sometimes inter-facility transfers from non-hospital settings). Thus, alternative methods of screening for GI dysfunction that do not subject patients to radiation and transport risk are highly desirable. One such emerging option that may help with bedside decision-making is GI point-of-care ultrasound (POCUS). However, utilization of GI POCUS is limited by a lack of training of providers and standardization in image acquisition and interpretation. To address this unmet need, we propose an evidence-based image acquisition protocol using point-of-care ultrasound to screen for two important types of GI dysfunction: ileus or obstruction. This protocol includes guidance on probe selection, patient positioning, and image acquisition sequence. Further, we review image interpretation and the limitations of this protocol.","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 221","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0