Malaysian Journal of Pathology最新文献_第4页

Chemoprevention of natural product against oral cancer: A comprehensive review. 天然产物对口腔癌的化学预防：综述。

IF 0.6 4区医学

Malaysian Journal of Pathology Pub Date : 2024-12-01

E A Alazazi, A Roslan, F M A Aziz, D Vanoh, A Seeni, S Ahmed, S Munir, J I Mohammad, M D Murtey

{"title":"Chemoprevention of natural product against oral cancer: A comprehensive review.","authors":"E A Alazazi, A Roslan, F M A Aziz, D Vanoh, A Seeni, S Ahmed, S Munir, J I Mohammad, M D Murtey","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: Oral cancer is considered the sixth most common form of cancer worldwide. It causes significant morbidity and mortality, especially in low socioeconomic status groups. However, Cancer chemoprevention encompasses the use of specific compounds to suppress the growth of tumours or inhibit carcinogenesis. Natural products have been identified as one of the most significant sources of anti-cancer agents. Meanwhile, several synthetic drugs exhibit potential cytotoxicity and can induce a wide range of degenerative diseases.Aim of the review: This review aims to determine the various plants, vegetables, and fruits possessing natural chemotherapeutic agents against oral cancer cells.Materials and methods: A comprehensive review of findings reported in articles retrieved from searches of computerised databases, hand searches, and authoritative texts. Inclusion databases include PubMed, Medline, Web of Science, Scopus, and Scientific. Exclusion Computerised databases: Wikipedia or unknown sources.Results: Natural products have fewer side effects, high selectivity, low toxicity, and eliminate cancer cells. Thus, the application of natural products as alternative oral and other cancer therapies has recently demonstrated remarkable progress.Conclusion: Natural products have been widely used in developing oral anti-cancer drugs. Most of these natural products present bioactive chemical agents and novel mechanisms of action, such as the inhibition of tumour cell growth, the induction of apoptosis, DNA damage, and the inhibition of topoisomerases I and II. In future, the successful integration of natural products in oral cancer chemoprevention field depends on the advancement of molecular targeting, personalised approaches, and the exploration of novel drug delivery systems. Furthermore, integration of preclinical findings in clinical trials will be important for translating research into impactful interventions.","PeriodicalId":48723,"journal":{"name":"Malaysian Journal of Pathology","volume":"46 3","pages":"355-368"},"PeriodicalIF":0.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of novel BCR::ABL1 kinase domain mutation in patients with chronic myeloid leukaemia and imatinib resistance. 慢性髓性白血病伴伊马替尼耐药患者新型BCR::ABL1激酶结构域突变的鉴定

IF 0.6 4区医学

Malaysian Journal of Pathology Pub Date : 2024-12-01

Y M Yusoff, Z A Seman, S Z Anoar, S S M Said, N Azman, N R Kamaluddin, J Abdullah, S S M Yacob, E Esa

{"title":"Identification of novel BCR::ABL1 kinase domain mutation in patients with chronic myeloid leukaemia and imatinib resistance.","authors":"Y M Yusoff, Z A Seman, S Z Anoar, S S M Said, N Azman, N R Kamaluddin, J Abdullah, S S M Yacob, E Esa","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: The emergence of mutations in the BCR::ABL1 kinase domain (KD) impairs imatinib mesylate (IM) binding capacity, thus contributing to IM resistance. Identification of these mutations is important for treatment decisions and precision medicine in chronic myeloid leukaemia (CML) patients. Our study aims to determine the frequency of BCR::ABL1 KD mutations in CML patients with IM resistance.Materials and methods: Twenty three CML patients (26.7%) showed to have BCR:ABL1 KD mutations with IM resistance.Results: A total of 14 different types of mutations were identified which are Y253H, E255K, T267A, A287T, M290R, F3111, T3151, F317L, F359V, F3591, F359C, K357T, A399T, E459K and two novel mutations; M290R and K357T. We also discovered two silent mutations at codons 389 and 401.Conclusion: Mutational analysis is recommended to identify patients at risk of disease progression. Therefore, early detection of such mutations may allow timely treatment intervention to prevent or overcome resistance.","PeriodicalId":48723,"journal":{"name":"Malaysian Journal of Pathology","volume":"46 3","pages":"431-439"},"PeriodicalIF":0.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oncolytic measles virus-induced cell killing in radio-resistant and drug-resistant nasopharyngeal carcinoma. 溶瘤性麻疹病毒在放射耐药和耐药鼻咽癌中诱导的细胞杀伤

IF 0.6 4区医学

Malaysian Journal of Pathology Pub Date : 2024-12-01

H K Looi, Y F Ngeow, L V Kiew, L Y Chang, H T Ong

{"title":"Oncolytic measles virus-induced cell killing in radio-resistant and drug-resistant nasopharyngeal carcinoma.","authors":"H K Looi, Y F Ngeow, L V Kiew, L Y Chang, H T Ong","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: The current first-line therapy for nasopharyngeal carcinoma (NPC) is often associated with long-term complications. Oncolytic measles virus (MV) therapy offers a promising alternative to cancer therapy. This study aims to investigate the efficacy of MV in killing NPC cells in vitro, both with or without resistance to radiation and drug therapy.Materials and methods: NPC cell lines, CNE-1, CNE-2, HONE-1 and C666-1, were exposed to repeated cycles of gamma-irradiation and cisplatin to establish radio- and chemo-resistant cell lines, respectively. The expression of MV receptors, CD46 and nectin-4, were assessed with flow cytometer. To test the efficacy of viral infection, parental and both resistant NPC cells were infected with Measles-GFP-NIS in vitro. The progress of syncytia spread on NPC cells was monitored with fluorescence microscopy up to 60-hours post-infection (p.i.). MV-mediated killing was assessed using tetrazolium-based cell viability assay.Results: We established cisplatin-resistant (CR) NPC cell lines that exhibit more than two-fold shift in IC50 against cisplatin. Only CNE-2 and C666-1 acquired resistant traits after a cumulative 60-Gy gamma irradiation. All untreated parental and resistant NPCs expressed CD46 but not nectin-4 on their cell surface and were susceptible to MV infection. Syncytia were observable as early as 24 hours p.i. and cell loss was observable at 48-hours p.i. onwards. Interestingly, Measles-GFP-NIS shows higher infectivity in NPC with resistance phenotypes, except in CR-C666-1, and were killed more compared to their non-resistant counterparts.Conclusion: Measles-GFP-NIS demonstrated potential as an alternative treatment in relapse, recurrent, or advanced stage NPC which often exhibits resistance towards chemo- and radiotherapy.","PeriodicalId":48723,"journal":{"name":"Malaysian Journal of Pathology","volume":"46 3","pages":"441-451"},"PeriodicalIF":0.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The positive impact of Streptococcus mutans on the growth of Candida albicans within mixed-species biofilms and implications to dental health. 变异链球菌对混合菌种生物膜中白色念珠菌生长的积极影响及其对牙齿健康的意义。

IF 0.6 4区医学

Malaysian Journal of Pathology Pub Date : 2024-08-01

X Wang, S F Yap, Y F Ngeow

引用次数: 0

Whole genome sequencing reveals the mutational landscape from disease diagnosis to relapse in patients with childhood acute myeloid leukaemia. 全基因组测序揭示了儿童急性髓性白血病患者从疾病诊断到复发期间的突变情况。

IF 0.6 4区医学

Malaysian Journal of Pathology Pub Date : 2024-08-01

H Aziz, N S Ab Mutalib, H Alias, R Jamal

{"title":"Whole genome sequencing reveals the mutational landscape from disease diagnosis to relapse in patients with childhood acute myeloid leukaemia.","authors":"H Aziz, N S Ab Mutalib, H Alias, R Jamal","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: Leukaemia is the most common cancer in children, however, there is still a big gap in knowledge about the genomic alterations in childhood acute myeloid leukaemia (AML) compared to adult AML. Relapsed AML remains as a leading cause of cancer deaths among children. This study aims to understand the molecular mechanisms of relapsed AML by elucidating the mutational landscape before and during relapse.Materials and methods: Whole genome sequencing was performed on matched samples collected at diagnosis, remission and relapse from three patients of de novo childhood AML. Sanger sequencing was performed for validation in 47 patients' samples, followed by functional analysis.Results: Overall, we identified 312 somatic mutations including synonymous single nucleotide variants (SNVs), missense SNVs, deletions and insertion frameshifts, stopgains and splice sites. After prioritisation, only 46 variants were present at diagnosis (13-17 mutations per patient) and 49 variants at relapse (12-20 mutations per patient). Out of 81 variants, there were 35 new variants detected at relapse but not present at diagnosis. Six potential driver mutations (KIT, CDC73, HNF1A, RBM10, ZMYM4 and ETV6) were identified in predicting relapse for the 3 patients, with recurrent mutations of the ETV6 gene in 2 patients. Functional analysis of the ETV6 mutation showed that ETV6 lost its tumour suppressive function when both mutant ETV6 p.P25fs and ETV6 p.N75fs were tested in vitro.Conclusion: This study has uncovered the mutational landscape in three local childhood AML patients and contributes to a better understanding of the molecular mechanisms of relapsed AML.","PeriodicalId":48723,"journal":{"name":"Malaysian Journal of Pathology","volume":"46 2","pages":"259-278"},"PeriodicalIF":0.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142113753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ChatGPT in pathology. 病理学中的 ChatGPT。

IF 0.6 4区医学

Malaysian Journal of Pathology Pub Date : 2024-08-01

G C Tan, Y P Wong

引用次数: 0

A rare case of compound heterozygous Southeast Asian double α-globin gene deletion and Haemoglobin Quong Sze in a Malay proband. 一例罕见的东南亚双α-球蛋白基因缺失和血红蛋白Quong Sze复合杂合子马来原型。

IF 0.6 4区医学

Malaysian Journal of Pathology Pub Date : 2024-08-01

D Vijian, W S Wan Ab Rahman, T P Kannan, Z Zulkafli, N H Mohd Noor, R Bahar, M Z Hassan, S Iberahim, M Abdullah, S Mohamed Yusoff, M Ramli, N Yasin, E Esa

{"title":"A rare case of compound heterozygous Southeast Asian double α-globin gene deletion and Haemoglobin Quong Sze in a Malay proband.","authors":"D Vijian, W S Wan Ab Rahman, T P Kannan, Z Zulkafli, N H Mohd Noor, R Bahar, M Z Hassan, S Iberahim, M Abdullah, S Mohamed Yusoff, M Ramli, N Yasin, E Esa","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: Haemoglobin (Hb) Quong Sze is a non-deletional α-thalassaemia subtype that occurs due to missense mutation at codon 125 of the HBA2 gene. Interaction between Hb QS with Southeast Asian double α-globin gene deletion results in non-deletional HbH disease, which is more severe than deletional HbH.Case report: A 3-month-old baby boy was presented with neonatal anaemia and mild hepatomegaly. Full blood count revealed severe hypochromic microcytic anaemia. There was an abundance of HbH inclusion bodies in his red blood cells. High-performance liquid chromatography showed a reduced HbA2 level with the presence of pre-run peak. Capillary electrophoresis showed the presence of HbH and Hb Barts. Molecular analysis found a common α0-thalassaemia (--SEA) in one allele and mutation in codon 125 in the other allele.Discussion: Non-deletional HbH disease due to a combination of deletional and non-deletional mutations may present with severe clinical manifestations than those with deletion mutations, which warrants accurate diagnosis using molecular techniques.","PeriodicalId":48723,"journal":{"name":"Malaysian Journal of Pathology","volume":"46 2","pages":"321-324"},"PeriodicalIF":0.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142113742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Congenital adrenal hyperplasia testing in the Malaysian population: real-world data sourced from a national reference laboratory. 马来西亚人口的先天性肾上腺皮质增生症检测：来自国家参考实验室的真实数据。

IF 0.6 4区医学

Malaysian Journal of Pathology Pub Date : 2024-08-01

R H Raja Bongsu, K Khalid, W A D Razali, N Zainal Abidin, N A I Saiful Nizam, N A Rahidin, S Apparow, A Habib

{"title":"Congenital adrenal hyperplasia testing in the Malaysian population: real-world data sourced from a national reference laboratory.","authors":"R H Raja Bongsu, K Khalid, W A D Razali, N Zainal Abidin, N A I Saiful Nizam, N A Rahidin, S Apparow, A Habib","doi":"","DOIUrl":"","url":null,"abstract":"Congenital Adrenal Hyperplasia (CAH) is primarily caused by mutations in genes responsible to produce enzymes involved in the synthesis of cortisol, aldosterone, or both. This study aims to determine the prevalence, sociodemographic distributions, and clinical factors associated with CAH in the Malaysian population. This retrospective study reviewed laboratory records of 17-hydroxyprogesterone (17OHP) test requests received at the Institute for Medical Research, Kuala Lumpur from January 2021 to December 2021. Descriptive statistics were employed for most variables, and logistic regression analysis was conducted to determine factors associated with CAH. The dataset included a total of 775 patients (64.2%) from 1,207 test requests screened. The prevalence of newly diagnosed CAH in the year 2021 was 13.5% (n=105). The majority were Malays (15.1%), neonates (13.8%), and females (45.7%). Higher baseline 17-OHP (cOR:1.31, 95% CI:1.19, 1.45), unknown gender at birth (cOR:7.82, 95% CI:2.86, 21.37), and neonatal age group at presentation (cOR:29.3, 95% CI:12.07, 71.03) independently predict CAH. The high prevalence of CAH in our region has been speculated to be due to the cultural consanguinity norms, resulting in genetic aberrations. CAH may manifest as ambiguous genitalia, particularly in females, due to the overproduction of androgens in-utero, resulting in atypical genitalia, necessitating thorough investigation. To the best of our knowledge, the data presented are the latest report on CAH prevalence, distribution, and description of positive CAH cases in the Malaysian population. These findings are essential for further public health planning to improve the diagnostic capacity and clinical management of CAH.","PeriodicalId":48723,"journal":{"name":"Malaysian Journal of Pathology","volume":"46 2","pages":"247-257"},"PeriodicalIF":0.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142113744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Family study of haemoglobin Arya in a Malaysian family. 马来西亚一个家庭的血红蛋白 Arya 家族研究。

IF 0.6 4区医学

Malaysian Journal of Pathology Pub Date : 2024-08-01

S Nahanthiran, N H Nik Mustapha, N Yasin, F B Idris, S B Md Noor

{"title":"Family study of haemoglobin Arya in a Malaysian family.","authors":"S Nahanthiran, N H Nik Mustapha, N Yasin, F B Idris, S B Md Noor","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: Thalassemia and haemoglobinopathies are relatively common among Malaysians. One of the rare haemoglobinopathies reported is Haemoglobin (Hb) Arya, which occurs due to substitution of aspartic acid at residue 47 of the alpha chain by asparagine. Here, we report the detection of Hb Arya in a Malaysian family, which was detected incidentally during family screening.Case report: A 16 years-old girl, clinically asymptomatic was noted to have low mean corpuscular haemoglobin (MCV) with normal Hb level. Hb analysis using capillary electrophoresis (CE) showed reduced Hb A of 76.5%, Hb A2 of 1.6% with presence of small peak at Zone 1 likely A2'. There was also a small peak noted at Hb D zone and Hb S zones which quantified as 1.5% and 20% respectively. Supplementary test by high performance liquid chromatography (HPLC) showed a prominent peak at D-window (19.6%) and a small peak at S-window (0.6%). DNA analysis revealed a heterozygous state of α2 codon 47 Hb Arya mutation. Subsequent family study showed a similar mutation in the father and sister of the index case.Conclusion: Very few reports are available up to date regarding Hb Arya. This report highlights the rare haemoglobinopathy in a Malay family in Malaysia that contributes to the growing literature of this rare haemoglobin variant.","PeriodicalId":48723,"journal":{"name":"Malaysian Journal of Pathology","volume":"46 2","pages":"315-320"},"PeriodicalIF":0.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142113745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rhabdomyolysis and vascular thrombosis supporting the electrocution related death. 横纹肌溶解症和血管栓塞是与电击有关的死亡的佐证。

IF 0.6 4区医学

Malaysian Journal of Pathology Pub Date : 2024-08-01

K L Chng, M M Umul Khairil Fatimah, Z Hafizatul Solehah, H Husna Syaza

{"title":"Rhabdomyolysis and vascular thrombosis supporting the electrocution related death.","authors":"K L Chng, M M Umul Khairil Fatimah, Z Hafizatul Solehah, H Husna Syaza","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: Electrocution related death remains an ambiguous judgement and requires numerous valid evidence for proper medico-legal diagnosis. While the presence of electrical burn marks is a significant macroscopic indicator, it can be absent, especially on moist skin. The electrical mark still represents a fundamental indicator above all in the medico-legal field, but the identification of pathognomonic elements and signs not limited to the skin alone could be a valid help in the future, especially in unclear cases.Case report: The deceased was brought-in-dead to the hospital from their workplace, with no signs of fatal natural diseases. External examination revealed a Y-shaped burn mark on the right side of the neck and collapsed blisters with greying rings on both heels. Internal examination showed no alarming findings. Further, histopathological analysis of the foot blisters and neck burn revealed intraepidermal detachment, elongated nuclei, and coagulative necrosis. Notably, the presence of muscle fibre casts in kidney tubules and microthrombi in lung sections which indicate rhabdomyolysis and vascular thrombosis supported electrocution-related death.Conclusion: These positive findings of the electrical burn marks externally and significant histopathological changes, collectively support the death was due to electrocution, after excluding any major, fatal injuries. Albeit, a detailed inspection of the crime scene plays an important role, in order to classify the electrocution related death.","PeriodicalId":48723,"journal":{"name":"Malaysian Journal of Pathology","volume":"46 2","pages":"331-337"},"PeriodicalIF":0.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142113749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0