Malaysian Journal of Pathology最新文献_第5页

A comparative analysis of blood and faecal-based laboratory methods in the diagnosis of extraintestinal microsporidia infection. 在诊断肠外微孢子虫感染时，对基于血液和粪便的实验室方法进行比较分析。

IF 0.6 4区医学

Malaysian Journal of Pathology Pub Date : 2024-08-01

P S Mohamed Yusoff, N Arifin, P Periyasamy, N R Tumian, F Ismail, R Z A Raja Sabudin, Z Md Idris, N W Hassan, E Osman

{"title":"A comparative analysis of blood and faecal-based laboratory methods in the diagnosis of extraintestinal microsporidia infection.","authors":"P S Mohamed Yusoff, N Arifin, P Periyasamy, N R Tumian, F Ismail, R Z A Raja Sabudin, Z Md Idris, N W Hassan, E Osman","doi":"","DOIUrl":"","url":null,"abstract":"Diagnosis of extraintestinal microsporidiosis is always hampered due to non-specific symptoms and difficulty in diagnosis. This study aimed to compare the diagnostic utility of blood and faecal-based polymerase chain reaction (PCR) to detect microsporidiosis in immunocompromised patients. A total of 42 immunocompromised patients consisting of HIV-infected and chemotherapy-treated patients were enrolled. Paired faecal and blood samples were collected and subjected to PCR to detect Enterocytozoon bieneusi and Encephalitozoon spp. Faecal samples were microscopically screened for microsporidia spores. Overall, 42.9% (18/42) of patients were positive for microsporidiosis. Of this, 19.0% (8/42) and 4.8% (2/42) were positive by blood and stool PCR respectively. Meanwhile, 33.3% (14/42) of the faecal specimens were microscopically positive. Among the positive patients, 22.2% (4/18) had microsporidia confirmed by blood PCR and stool microscopy, suggestive of dissemination. Interestingly, the stool specimen in which microsporidia spores were detected via microscopy is not positive via PCR method. This highlights the limitation of the faecal-based detection method and the important use of blood samples for diagnosing extraintestinal microsporidiosis. Only E. bieneusi species were detected in all PCR-positive samples. This study highlights the diagnostic value of blood PCR in diagnosing extraintestinal microsporidiosis infections.","PeriodicalId":48723,"journal":{"name":"Malaysian Journal of Pathology","volume":"46 2","pages":"299-306"},"PeriodicalIF":0.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142113741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A 10-year sociodemographic study of suicide cases. 为期 10 年的自杀案例社会人口学研究。

IF 0.6 4区医学

Malaysian Journal of Pathology Pub Date : 2024-08-01

M I Jamaludin, W H Hafiz, R S Shatnawi, M S Shafee, F Mohd Nor

{"title":"A 10-year sociodemographic study of suicide cases.","authors":"M I Jamaludin, W H Hafiz, R S Shatnawi, M S Shafee, F Mohd Nor","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: The prevalence of suicide presents a significant public health challenge globally, affecting diverse populations around the world. This study is motivated by the urgency to tackle suicide as a crucial public health issue on a global level, according to the United Nations Sustainable Development Goals and the General Programme of Work and Mental Health Action by the World Health Organization.Materials and methods: The research was performed at the Forensic Unit of Hospital Canselor Tuanku Muhriz, spanning a retrospective analysis covering 2012 to 2021. A descriptive analysis was conducted to analyse the patterns of suicide and the sociodemographic characteristics of the cases.Results: The findings show monthly variations and a recent increase in suicide rates, especially during the pandemic of COVID-19. Analysis of suicide methods indicates that hanging is the most common suicide method, which accounts for almost half of the sample population, followed by jumping from a height and burning charcoal. Adult males are primarily implicated in suicidal acts, and the study reveals unique trends among different age groups, genders and employment statuses.Conclusion: This study aims to offer thorough insights and comprehension into suicide within the Malaysian sociodemographic framework. Consequently, it could furnish valuable insights for public health authorities and governmental bodies.","PeriodicalId":48723,"journal":{"name":"Malaysian Journal of Pathology","volume":"46 2","pages":"287-293"},"PeriodicalIF":0.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142113740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Upregulation of miR-424 inhibit retinal endothelial cells proliferation under high glucose condition via cyclin D1. miR-424 的上调可通过细胞周期蛋白 D1 抑制高糖条件下视网膜内皮细胞的增殖。

IF 0.6 4区医学

Malaysian Journal of Pathology Pub Date : 2024-08-01

Y Chen, K Chen, W Zhu, J Chen, Z Huang

{"title":"Upregulation of miR-424 inhibit retinal endothelial cells proliferation under high glucose condition via cyclin D1.","authors":"Y Chen, K Chen, W Zhu, J Chen, Z Huang","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: Diabetic retinopathy is characterised by retinal vascular impairment. A number of aberrant microRNAs (miRNAs) have a role in the pathophysiology of vascular dysfunction. However, the relevance of miR-424 in retinal vascular endothelial cell dysfunction during hyperglycemia stress remains unknown. The purpose of this study is to investigate this issue.Materials and methods: Rhesus macaque choroid retinal endothelial cell line (RF/6A) cells were cultivated in normal glucose (NG) and high glucose (HG) conditions. The mRNA expression of miR-424 and Cyclin D1 (CCND1) was quantified using qPCR, and the protein quantity of CCND1 was detected using Western Blot. miR-424 mimics, miR-424 inhibitors, miR-424 inhibitor+ siRNA-CCND1 or vehicle molecules were transfected into RF/6A cells. MTT test was used to assess cell proliferation, and flow cytometric analysis was used to assess cell cycle. The interaction between miR-424 and CCND1 was predicted using bioinformatics and validated using dual luciferase reporter analysis.Results: miR-424 was up-regulated, and cell viability was reduced in HG compared to NG. By reversing the expression of miR-424 in certain situations, the phenotypes can be changed. CCND1 has been identified as a miR-424 target gene, and it may be regulated at the transcriptional and translational levels. Manipulation of silencing CCND1 can counteract the effect of transfecting miR-424 inhibitor into RF/6A cells under HG such as proliferation stimulation.Conclusions: Our findings indicate that miR-424 plays an important role in hyperglycemia induced ARPE-19 cells damage, and it could be a new therapeutic target for DR by preventing retinal vascular cells from HG-induced injury.","PeriodicalId":48723,"journal":{"name":"Malaysian Journal of Pathology","volume":"46 2","pages":"279-286"},"PeriodicalIF":0.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142113752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FOXP3+ regulatory T cells, mismatch repair proteins and BRAF V600E status in young-onset colorectal cancer. 年轻患者结直肠癌中的 FOXP3+ 调节性 T 细胞、错配修复蛋白和 BRAF V600E 状态。

IF 0.6 4区医学

Malaysian Journal of Pathology Pub Date : 2024-08-01

R Isah Tsamiya, S N Mohd Nafi, N A Che Jalil, A A Mat Zin

{"title":"FOXP3+ regulatory T cells, mismatch repair proteins and BRAF V600E status in young-onset colorectal cancer.","authors":"R Isah Tsamiya, S N Mohd Nafi, N A Che Jalil, A A Mat Zin","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: Young onset colorectal cancer (YOCRC) is a heterogenous CRC phenotype with an increasing trend globally. This study aims to determine FOXP3+ Treg cells, Mismatch Repair (MMR) proteins, and proto-oncogene B-Raf (BRAF) V600E status among YOCRC patients at Hospital Universiti Sains Malaysia.Materials and methods: This was a retrospective study of YOCRC (<50 years) over 8 years (January 2013 to December 2021). Immunohistochemistry staining of FOXP3, BRAFV600E, and MMR protein expression was performed using monoclonal antibodies. The staining intensity and percentage of positive cells were used to evaluate the staining using immunoreactive scoring. All data were analysed using descriptive and correlation statistics. A p-value of ≤ 0.05 was taken as statistically significant.Results: A total of 65 YOCRC patients were diagnosed, out of which 53.8% had proficient MMR (pMMR) with a mean age of 41, while 46.2% had deficient MMR (dMMR) with a mean age of 35.5. The pMMR with the BRAFV600E+ group expressed higher FOXP3+Tregs (54.2%) than the dMMR with the BRAFV600E+ group (22.9%). Patients with lower FOXP3+Tregs were observed more in dMMR with BRAFV600E- (47%) than in pMMR with BRAFV600E- (5.9%). There was a statistically significant association between the density of expressed FOXP3+Tregs with MMR and BRAFV600E status (p=0.002).Conclusion: While most of the YOCRC had pMMR, others exhibited dMMR with loss of one or more MMR proteins. The presence of BRAFV600E demonstrated the YOCRC's sporadic nature. A high FOXP3+Treg expression was significantly associated with MMR and BRAFV600E status. Future research must be expanded to cover other hospitals to increase the sample size and include MLH1 hypermethylation testing.","PeriodicalId":48723,"journal":{"name":"Malaysian Journal of Pathology","volume":"46 2","pages":"233-246"},"PeriodicalIF":0.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142113746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The rate of blood culture contamination and common organisms isolated in Malaysian public hospitals. 马来西亚公立医院血液培养污染率和常见分离菌。

IF 0.6 4区医学

Malaysian Journal of Pathology Pub Date : 2024-08-01

N A Mokhtar, S Y Ting, N Z Zainol Abidin, A Abdul Hameed, Z Mohamed, N I Mustapa, N H Ahmad, S Baharuddin, G Sibalinggam, Z L Abd Hadi, Z Zam, L A Mat Nor, S F Situ, F D Sharuddin, A S Mohd Ali, N Abdullah Azahari, R Rashid, S S Syed Omar, N Othman, N R Rasid, P Thangarajah, K Y Boon, N A Mohd Said, M Z Mohd Zaili, A H Arba'Eni, R Baharudin, K Md Noor, W A Wan Ismail, P Y Heng, W A A Ahmad Ghazali, S N Khamis, H Baharim

{"title":"The rate of blood culture contamination and common organisms isolated in Malaysian public hospitals.","authors":"N A Mokhtar, S Y Ting, N Z Zainol Abidin, A Abdul Hameed, Z Mohamed, N I Mustapa, N H Ahmad, S Baharuddin, G Sibalinggam, Z L Abd Hadi, Z Zam, L A Mat Nor, S F Situ, F D Sharuddin, A S Mohd Ali, N Abdullah Azahari, R Rashid, S S Syed Omar, N Othman, N R Rasid, P Thangarajah, K Y Boon, N A Mohd Said, M Z Mohd Zaili, A H Arba'Eni, R Baharudin, K Md Noor, W A Wan Ismail, P Y Heng, W A A Ahmad Ghazali, S N Khamis, H Baharim","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: Blood culture contamination remains a dilemma issue in the diagnosis of bloodstream infection. However, to date, there is no national data on blood culture contamination and the common organism isolated in Malaysia. This is a pioneer multi-centre study involving public hospitals with medical microbiologists in Malaysia to determine the blood culture contamination rate and the common organism isolated.Materials and methods: This retrospective cross-sectional study involved record review of all blood culture results over 9 months period from 1st January 2018 until 30th September 2018 in 27 government hospitals in Malaysia. For each positive culture result, the type of isolated organism was classified to represent true bacteraemia or contamination.Results: We analysed 448,109 blood culture records from the participating hospitals. The blood culture positivity rate was 12.5% (57395 of 448109) and 25.0% (14367 of 57395) of the positive blood culture represents contamination. The national blood culture contamination rate in Malaysia was 3.2%. The contamination rate in the adult population was significantly higher than the paediatric population (3.6% vs. 2.6%; p<0.001). The blood contamination rate by institution ranged from 1.5% to 6.8%. The most frequently isolated microorganisms in the contaminated cultures were coagulase-negative staphylococci (71.0%).Conclusion: Blood culture contamination is a major issue that warrants priority in recognition, and interventions should be implemented to reduce the blood contamination rate in Malaysia.","PeriodicalId":48723,"journal":{"name":"Malaysian Journal of Pathology","volume":"46 2","pages":"307-314"},"PeriodicalIF":0.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142113751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Marrow talaromycosis as the initial presentation in a case of Burkitt lymphoma. 一例伯基特淋巴瘤患者以骨髓滑液菌病为首发症状。

IF 0.6 4区医学

Malaysian Journal of Pathology Pub Date : 2024-08-01

N E Hasbullah, R Z A Raja Sabudin, A S Asri, N Yusof, C F Leong, F Mohammed, C H Ding, N R Tumian

{"title":"Marrow talaromycosis as the initial presentation in a case of Burkitt lymphoma.","authors":"N E Hasbullah, R Z A Raja Sabudin, A S Asri, N Yusof, C F Leong, F Mohammed, C H Ding, N R Tumian","doi":"","DOIUrl":"","url":null,"abstract":"Talaromyces marneffei is a thermally dimorphic fungus which causes opportunistic infections in immunocompromised individuals. The diagnosis of T. marneffei infection rests on the microscopic demonstration of the fungus in the tissues and/or isolation of the fungus from clinical specimens. In this report, we discuss a case involving a 23-year-old man who presented with a history of intermittent fever, cough and constitutional symptoms. Clinically, the patient exhibited pallor, jaundice, generalized seborrhoeic dermatitis, hepatomegaly, and small palpable cervical lymph nodes. A computed tomography (CT) scan of the abdomen showed homogenous hypodense lesions in both liver lobes. HIV screening result was reactive. Microscopic examination of the bone marrow aspirate smear and trephine biopsy identified fungal bodies, and culture of the marrow aspirate confirmed the presence of T. marneffei. Notably, the liver biopsy revealed Burkitt lymphoma alongside fungal bodies. He was treated with intravenous Amphotericin B but ultimately succumbed to the illness due to severe metabolic acidosis and multiorgan failure. This case underscores the importance of presumptive diagnosis through morphological or histological examination of bone marrow samples, as microbiologic culture methods can be time-consuming. Timely diagnosis and aggressive treatment are critical in managing patients with T. marneffei infection.","PeriodicalId":48723,"journal":{"name":"Malaysian Journal of Pathology","volume":"46 2","pages":"325-329"},"PeriodicalIF":0.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142113747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Residual post-treatment rhabdomyosarcoma in bone marrow: A reminder of the continued importance of morphology. 骨髓中残留的治疗后横纹肌肉瘤：提醒人们注意形态学的持续重要性。

IF 0.6 4区医学

Malaysian Journal of Pathology Pub Date : 2024-08-01

O Abobarin-Aofolaju, B Kaumeyer, S B Kahwash

引用次数: 0

Abstracts of the 12th Congress of the Asian-Pacific Society of Thrombosis and Haemostasis (APSTH) 2023 "Haemostasis and Thrombosis: Bench to bedside", was held at Borneo Convention Centre Kuching (BCCK), Sarawak on 18th to 21st October 2023. 2023 年亚太血栓与止血学会（APSTH）第 12 届大会 "止血与血栓：从实验室到床边 "摘要于 2023 年 10 月 18 日至 21 日在砂拉越古晋婆罗洲会议中心（BCCK）举行。

IF 1.8 4区医学

Malaysian Journal of Pathology Pub Date : 2024-04-01

引用次数: 0

Navigating an unexpected diagnosis - experience of a tertiary referral centre with two cases of intraplacental choriocarcinoma. 应对意外诊断--一家三级转诊中心对两例胎盘内绒毛膜癌的经验。

IF 1.8 4区医学