Microscopy最新文献_第2页

Detection of Autophagy-Related Structures in Fruiting Bodies of Edible Mushroom, Pleurotus ostreatus. 食用菌平菇子实体中自噬相关结构的检测。

IF 1.8 4区工程技术

Microscopy Pub Date : 2022-04-21 DOI: 10.1093/jmicro/dfac020

Yuma Ozaki, T. Aimi, N. Shimomura

{"title":"Detection of Autophagy-Related Structures in Fruiting Bodies of Edible Mushroom, Pleurotus ostreatus.","authors":"Yuma Ozaki, T. Aimi, N. Shimomura","doi":"10.1093/jmicro/dfac020","DOIUrl":"https://doi.org/10.1093/jmicro/dfac020","url":null,"abstract":"Autophagy is involved in various fungal morphogenetic processes. However, there are limited reports regarding the role of autophagy in mushroom fruiting body formation. The purpose of this study was to reveal the autophagy-related structures in mushroom-forming fungi. The edible mushroom Pleurotus ostreatus was used in this study. Transmission electron microscopy revealed double-membrane bounded structures containing cytoplasmic components in the fruiting bodies of this fungus. Some of these double-membrane structures were observed to interact with the vacuoles. Additionally, curved flat cisternae of various lengths were detected in the cytoplasm. The shape, size, and thickness of the limiting membrane of the double-membrane structures and the flat cisternae corresponded well with those of the autophagosomes and the isolation membranes, respectively. Regarding autophagosome formation, a membrane-bound specific zone was detected near the isolation membrane, which appeared to expand along the novel membrane. This is the first detailed report showing autophagy-related structures in P. ostreatus and provides a possible model for autophagosome formation in these filamentous fungi. Mini-abstract Autophagy is involved in fungal morphogenetic processes. The fruiting bodies of edible mushroom Pleurotus ostreatus was observed under a TEM. The present study showed autophagy-related structures in this fungus and provides a possible model for autophagosome formation in filamentous fungi.","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2022-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44591942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Ultrastructural characteristics of finger-like membrane protrusions in cell competition 细胞竞争中指状膜突起的超微结构特征

IF 1.8 4区工程技术

Microscopy Pub Date : 2022-04-08 DOI: 10.1093/jmicro/dfac017

Tomoko Kamasaki, Ryota Uehara, Yasuyuki Fujita

{"title":"Ultrastructural characteristics of finger-like membrane protrusions in cell competition","authors":"Tomoko Kamasaki, Ryota Uehara, Yasuyuki Fujita","doi":"10.1093/jmicro/dfac017","DOIUrl":"https://doi.org/10.1093/jmicro/dfac017","url":null,"abstract":"Abstract A small number of oncogenic mutated cells sporadically arise within the epithelial monolayer. Newly emerging Ras- or Src-transformed epithelial cells are often apically eliminated during competitive interactions between normal and transformed cells. Our recent electron microscopy (EM) analyses revealed that characteristic finger-like membrane protrusions are formed at the interface between normal and RasV12-transformed cells via the cdc42–formin-binding protein 17 (FBP17) pathway, potentially playing a positive role in intercellular recognition during apical extrusion. However, the spatial distribution and ultrastructural characteristics of finger-like protrusions remain unknown. In this study, we performed both X–Y and X–Z EM analyses of finger-like protrusions during the apical extrusion of RasV12-transformed cells. Quantification of the distribution and widths of the protrusions showed comparable results between the X–Y and X–Z sections. Finger-like protrusions were observed throughout the cell boundary between normal and RasV12 cells, except for apicalmost tight junctions. In addition, a non-cell-autonomous reduction in protrusion widths was observed between RasV12 cells and surrounding normal cells under the mix culture condition. In the finger-like protrusions, intercellular adhesions via thin electron-dense plaques were observed, implying that immature and transient forms of desmosomes, adherens junctions or unknown weak adhesions were distributed. Interestingly, unlike RasV12-transformed cells, Src-transformed cells form fewer evident protrusions, and FBP17 in Src cells is dispensable for apical extrusion. Collectively, these results suggest that the dynamic reorganization of intercellular adhesions via finger-like protrusions may positively control cell competition between normal and RasV12-transformed cells. Furthermore, our data indicate a cell context–dependent diversity in the modes of apical extrusion.","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":"71 1","pages":"195 - 205"},"PeriodicalIF":1.8,"publicationDate":"2022-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46296015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of aging on basement membrane of tibialis anterior muscle during recovery following muscle injury in rats 衰老对大鼠胫骨前肌基底膜损伤恢复期的影响

IF 1.8 4区工程技术

Microscopy Pub Date : 2022-03-29 DOI: 10.1093/jmicro/dfac016

Yuji Kanazawa, M. Nagano, S. Koinuma, S. Sugiyo, Y. Shigeyoshi

引用次数: 3

Effect of Amorphous Carbon Coating on the Performance of Liquid Phase Transmission Electron Microscopy (LP-TEM) and the Dynamics of Enclosed Pt Nano-Colloids. 非晶态碳涂层对液相透射电子显微镜（LP-TEM）性能和封闭Pt纳米胶体动力学的影响。

IF 1.8 4区工程技术

Microscopy Pub Date : 2022-03-11 DOI: 10.1093/jmicro/dfac012

Xiaoguang Li, K. Mitsuishi, M. Takeguchi

引用次数: 1

Single-molecule Observation of Self-Propagating Amyloid Fibrils. 自繁殖淀粉样纤维的单分子观察。

IF 1.8 4区工程技术

Microscopy Pub Date : 2022-03-07 DOI: 10.1093/jmicro/dfac011

T. Watanabe-Nakayama, K. Ono

{"title":"Single-molecule Observation of Self-Propagating Amyloid Fibrils.","authors":"T. Watanabe-Nakayama, K. Ono","doi":"10.1093/jmicro/dfac011","DOIUrl":"https://doi.org/10.1093/jmicro/dfac011","url":null,"abstract":"The assembly of misfolded proteins into amyloid fibrils is associated with amyloidosis, including neurodegenerative diseases, such as Alzheimer's, Parkinson's, and prion diseases. The self-propagation of amyloid fibrils is widely observed in the aggregation pathways of numerous amyloidogenic proteins. This propensity with plasticity in primary nucleation allows amyloid fibril polymorphism, which is correlated with the pathology/phenotypes of patients. Because the interference with the nucleation and replication processes of amyloid fibrils can alter the amyloid structure and the outcome of the disease, these processes can be a target for developing clinical drugs. Single-molecule observation of amyloid fibril replication can be an experimental system to provide the kinetic parameters for simulation studies and confirm the effect of clinical drugs. Here, we review single-molecule observation of the amyloid fibril replication process using fluorescence microscopy and time-lapse atomic force microscopy, including high-speed atomic force microscopy. We discussed the amyloid fibril replication process and combined single-molecule observation results with molecular dynamics simulations. Mini Abstract Structural dynamics in amyloid aggregation is related with various Alzheimer's and Parkinson's disease symptoms. Single-molecule observation using high-speed atomic force microscopy can directly visualize the structural dynamics of individual amyloid aggregate assemblies. Here, we review historical and recent studies of single-molecule observation of amyloid aggregation with supportive molecular dynamics simulation.","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":"1 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2022-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41614649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Scanning electron microscopy of Escherichia coli encapsulated in a spacerized graphene sandwich. 用扫描电子显微镜观察包裹在石墨烯夹层中的大肠杆菌。

IF 1.8 4区工程技术

Microscopy Pub Date : 2022-02-26 DOI: 10.1093/jmicro/dfac010

Yuki Sasaki, S. Hirayama, R. Nakao

引用次数: 0

Advances in ultrahigh-energy resolution EELS: phonons, infrared plasmons and strongly coupled modes. 超高能量分辨率EELS的进展：声子、红外等离子体激元和强耦合模式。

IF 1.8 4区工程技术

Microscopy Pub Date : 2022-02-18 DOI: 10.1093/jmicro/dfab050

M. Lagos, I. Bicket, S. Mousavi M, G. Botton

{"title":"Advances in ultrahigh-energy resolution EELS: phonons, infrared plasmons and strongly coupled modes.","authors":"M. Lagos, I. Bicket, S. Mousavi M, G. Botton","doi":"10.1093/jmicro/dfab050","DOIUrl":"https://doi.org/10.1093/jmicro/dfab050","url":null,"abstract":"Nowadays, sub-50 meV atom-wide electron probes are routinely produced for electron energy loss spectroscopy in transmission electron microscopes due to monochromator technology advances. We review how gradual improvements in energy resolution enabled the study of very low-energy excitations such as lattice phonons, molecular vibrations, infrared plasmons and strongly coupled hybrid modes in nanomaterials. Starting with the theoretical framework needed to treat inelastic electron scattering from phonons in solids, we illustrate contributions in detecting optical surface phonons in photonic structures. We discuss phonon mapping capabilities in real and reciprocal space, and the localized phonon response near nano-/atomic-scale structural features. We also survey the progress of aloof spectroscopy in studying vibrations in organic materials and applications in measuring local temperature and photonic density of states in single nanostructures using phonon scattering. We then turn towards studies on infrared plasmons in metals and semiconductors. Spectroscopy analyses now extend towards probing extremely complex broadband platforms, the effects of defects and nanogaps, and some far-reaching investigations towards uncovering plasmon lifetime and 3D photonic density of states. In doped semiconductors, we review research on the use of the electron probe to correlate local doping concentration and atomic-scale defects with the plasmonic response. Finally, we discuss advances in studying strong coupling phenomena in plasmon-exciton and plasmon-phonon systems. Overall, the wealth of information gained extends our knowledge about nanomaterial properties and elementary excitations, illustrating the powerful capabilities of high-energy resolution scanning transmission electron microscopy-electron energy loss spectrometry.","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":"71 Supplement_1 1","pages":"i174-i199"},"PeriodicalIF":1.8,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44622865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

The 70th anniversary issue: Preface. 70周年纪念:前言。

IF 1.8 4区工程技术

Microscopy Pub Date : 2022-02-18 DOI: 10.1093/jmicro/dfab056

S. Okabe

引用次数: 0

Imaging neural circuit pathology of autism spectrum disorders: autism-associated genes, animal models and the application of in vivo two-photon imaging. 自闭症谱系障碍的神经回路病理成像:自闭症相关基因、动物模型和体内双光子成像的应用。

IF 1.8 4区工程技术

Microscopy Pub Date : 2022-02-18 DOI: 10.1093/jmicro/dfab039

H. Terashima, Keiichiro Minatohara, Hisato Maruoka, S. Okabe

引用次数: 1

Electron microscopic visualization of single molecules by tag-mediated metal particle labeling. 通过标签介导的金属颗粒标记的单分子的电镜可视化。

IF 1.8 4区工程技术

Microscopy Pub Date : 2022-02-18 DOI: 10.1093/jmicro/dfab048

R. Shigemoto

{"title":"Electron microscopic visualization of single molecules by tag-mediated metal particle labeling.","authors":"R. Shigemoto","doi":"10.1093/jmicro/dfab048","DOIUrl":"https://doi.org/10.1093/jmicro/dfab048","url":null,"abstract":"Genetically encoded tags have introduced extensive lines of application from purification of tagged proteins to their visualization at the single molecular, cellular, histological and whole-body levels. Combined with other rapidly developing technologies such as clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system, proteomics, super-resolution microscopy and proximity labeling, a large variety of genetically encoded tags have been developed in the last two decades. In this review, I focus on the current status of tag development for electron microscopic (EM) visualization of proteins with metal particle labeling. Compared with conventional immunoelectron microscopy using gold particles, tag-mediated metal particle labeling has several advantages that could potentially improve the sensitivity, spatial and temporal resolution, and applicability to a wide range of proteins of interest (POIs). It may enable researchers to detect single molecules in situ, allowing the quantitative measurement of absolute numbers and exact localization patterns of POI in the ultrastructural context. Thus, genetically encoded tags for EM could revolutionize the field as green fluorescence protein did for light microscopy, although we still have many challenges to overcome before reaching this goal.","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":"71 Supplement_1 1","pages":"i72-i80"},"PeriodicalIF":1.8,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60920361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1