Microscopy最新文献_第3页

Analytical technique for self-absorption structure of iron L-emission spectra obtained by soft X-ray emission spectrometer 软x射线发射光谱仪获得的铁l发射光谱的自吸收结构分析技术

IF 1.8 4区工程技术

Microscopy Pub Date : 2022-02-17 DOI: 10.1093/jmicro/dfac009

T. Yokoyama, H. Takahashi, S. Koshiya, T. Murano, M. Terauchi

{"title":"Analytical technique for self-absorption structure of iron L-emission spectra obtained by soft X-ray emission spectrometer","authors":"T. Yokoyama, H. Takahashi, S. Koshiya, T. Murano, M. Terauchi","doi":"10.1093/jmicro/dfac009","DOIUrl":"https://doi.org/10.1093/jmicro/dfac009","url":null,"abstract":"Abstract The method deriving the L self-absorption spectrum from Lα,β emission spectra obtained at different accelerating voltages has been optimized for analyzing the chemical state of Fe in solid materials. Fe Lα,β emission spectra obtained are fitted using Pseudo-Voigt functions and normalized by the integrated intensity of each Fe Ll line, which is not affected by L2,3 absorption edge. The self-absorption spectrum is calculated by dividing the normalized intensity profile collected at low accelerating voltage by that collected at a higher accelerating voltage. The obtained profile is referred to as soft X-ray self-absorption structure (SX-SAS). This method is applied to six Fe-based materials (Fe metal, FeO, Fe3O4, Fe2O3, FeS and FeS2) to observe different chemical states of Fe in those materials. By comparing the self-absorption spectra of iron oxides, one can observe the L3 absorption peak structure shows a shift to the higher energy side as ferric (3+) Fe increases with respect to ferrous (+2) Fe. The intensity profiles of self-absorption spectra of metallic Fe and FeS2 shows shoulder structures between the L3 and L2 absorption peaks, which were not observed in spectra of Fe oxides. These results indicate that the SX-SAS technique is useful to examine X-ray absorption structure as a means to understand the chemical states of transition metal elements.","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":"71 1","pages":"169 - 174"},"PeriodicalIF":1.8,"publicationDate":"2022-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47496946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Classification for transmission electron microscope images from different amorphous states using persistent homology 利用持久同源性对不同非晶态透射电子显微镜图像进行分类

IF 1.8 4区工程技术

Microscopy Pub Date : 2022-02-16 DOI: 10.1093/jmicro/dfac008

Fumihiko Uesugi, M. Ishii

引用次数: 1

Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes 维甲酸X受体的抑制改善了三维培养的小鼠角质形成细胞的形态、桥粒蛋白的定位和细胞旁通透性

IF 1.8 4区工程技术

Microscopy Pub Date : 2022-02-16 DOI: 10.1093/jmicro/dfac007

Shoko Ishikawa, M. Nikaido, Takahito Otani, Kayoko Ogata, H. Iida, Yuko Inai, Sachio Tamaoki, T. Inai

{"title":"Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes","authors":"Shoko Ishikawa, M. Nikaido, Takahito Otani, Kayoko Ogata, H. Iida, Yuko Inai, Sachio Tamaoki, T. Inai","doi":"10.1093/jmicro/dfac007","DOIUrl":"https://doi.org/10.1093/jmicro/dfac007","url":null,"abstract":"Abstract Retinoic acid (RA) plays an important role in epithelial homeostasis and influences the morphology, proliferation, differentiation and permeability of epithelial cells. Mouse keratinocytes, K38, reconstituted non-keratinized stratified epithelium in three-dimensional (3D) cultures with serum, which contains retinol (a source of RA), but the morphology was different from in vivo epithelium. The formed epithelium was thick, with loosened cell–cell contacts. Here, we investigated whether the inhibition of RA receptor (RAR)/retinoid X receptor (RXR)-mediated signaling by an RXR antagonist, HX 531, improved K38 3D cultures in terms of morphology and intercellular junctions. The epithelium formed by 0.5 μM HX531 was thin, and the intercellular space was narrowed because of the restoration of the layer-specific distribution of desmoglein (DSG)-1, DSG3 and plakoglobin (PG). Moreover, the levels of desmosomal proteins and tight junction proteins, including DSG1, DSG2, DSG3, PG, claudin (CLDN)-1 and CLDN4 increased, but the adherens junction protein, E-cadherin, did not show any change. Furthermore, CLDN1 was recruited to occludin-positive cell–cell contacts in the superficial cells and transepithelial electrical resistance was increased. Therefore, K38 3D cultures treated with 0.5 μM HX531 provides a useful in vitro model to study intercellular junctions in the non-keratinized epithelium.","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":"71 1","pages":"152 - 160"},"PeriodicalIF":1.8,"publicationDate":"2022-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42852543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine-learning-based quality-level-estimation system for inspecting steel microstructures 基于机器学习的钢组织检测质量水平估计系统

IF 1.8 4区工程技术

Microscopy Pub Date : 2021-07-25 DOI: 10.1093/jmicro/dfac019

Hiromi Nishiura, A. Miyamoto, Akira Ito, Shogo Suzuki, Kouhei Fujii, Hiroshi Morifuji, Hiroyuki Takatsuka

引用次数: 2

OUP accepted manuscript OUP接受稿件

IF 1.8 4区工程技术

Microscopy Pub Date : 2021-01-01 DOI: 10.1093/jmicro/dfab032

引用次数: 0

Local surface plasmon resonance of gold nanoparticles as a correlative light and electron microscopy (CLEM) tag for biological samples. 金纳米粒子的局部表面等离子体共振作为生物样品的相关光学和电子显微镜(CLEM)标签。

IF 1.8 4区工程技术

Microscopy Pub Date : 2019-12-03 DOI: 10.1093/jmicro/dfz031

Tomohiro Haruta, Keiko Hasumi, Y. Ikeda, Y. Konyuba, T. Fukuda, H. Nishioka

引用次数: 4

Elastic fiber system evaluated in the digestive organ of rats. 在大鼠消化器官中评估弹性纤维系统。

IF 1.8 4区工程技术

Microscopy Pub Date : 2019-12-03 DOI: 10.1093/jmicro/dfz030

Kouji Inoue, N. Kuroda, Tetsuji Sato

{"title":"Elastic fiber system evaluated in the digestive organ of rats.","authors":"Kouji Inoue, N. Kuroda, Tetsuji Sato","doi":"10.1093/jmicro/dfz030","DOIUrl":"https://doi.org/10.1093/jmicro/dfz030","url":null,"abstract":"According to our previous reports, the intraperiodontal elastic fiber system comprises oxytalan fibers, whereas all types of elastic system fibers are present in the gingiva. Much remains to be elucidated regarding the topographic development of the elastic fiber system that constitutes the walls of the digestive organs. This study aimed to examine the topographic development of the elastic fiber system in the periodontal tissue, oral cavity and digestive tract of rats at light- and electron microscopic levels. At embryonic day 20, in situ hybridization revealed the mRNA expression of tropoelastin in the putative gingival lamina propria but not in the dental follicle. At the postnatal stage, the masticatory mucous membrane of the gingiva and hard palate comprised three different types of elastic system fibers (oxytalan, elaunin and elastic fibers). Conversely, the elastic fiber system comprised elaunin and elastic fibers in other oral mucosae and the lining mucosae of digestive tract organs (the esophagus, stomach and small intestine). The findings of our study suggest that the elastic fiber system is mainly related to tissue resistance in the periodontal ligament and tissue elasticity in the oral mucosae without masticatory mucosae and the overlying mucosa of digestive tracts and both functions in the gingiva and hard palate, respectively. The appearance of elaunin fibers in the periodontium of rats aged 14 weeks suggests the expression of tropoelastin induced by mechanical stressors such as mastication. The intraperiodontal difference in the distribution of elaunin fibers suggests heterogeneity among fibroblasts constituting the periodontium.","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2019-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfz030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49533922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Atomistic and dynamic structural characterizations in low-dimensional materials: recent applications of in situ transmission electron microscopy. 低维材料的原子和动态结构表征：原位透射电子显微镜的最新应用。

IF 1.8 4区工程技术

Microscopy Pub Date : 2019-11-18 DOI: 10.1093/jmicro/dfz038

He Zheng, Fan Cao, Ligong Zhao, Renhui Jiang, Peili Zhao, Ying Zhang, Yanjie Wei, S. Meng, Kaixuan Li, Shuangfeng Jia, Luying Li, Jianbo Wang

引用次数: 2

Properties of electrostatic correcting systems with annular apertures. 环形孔径静电校正系统的性能。

IF 1.8 4区工程技术

Microscopy Pub Date : 2019-11-11 DOI: 10.1093/jmicro/dfz035

T. Kodama, T. Kawasaki, T. Ikuta

引用次数: 0

Sample preparation utilizing sputter coating increases contrast of cellulose nanocrystals in the transmission electron microscope. 利用溅射涂层制备样品增加了纤维素纳米晶体在透射电子显微镜下的对比度。