MicroscopyPub Date : 2019-12-03DOI: 10.1093/jmicro/dfz031
Tomohiro Haruta, Keiko Hasumi, Y. Ikeda, Y. Konyuba, T. Fukuda, H. Nishioka
{"title":"Local surface plasmon resonance of gold nanoparticles as a correlative light and electron microscopy (CLEM) tag for biological samples.","authors":"Tomohiro Haruta, Keiko Hasumi, Y. Ikeda, Y. Konyuba, T. Fukuda, H. Nishioka","doi":"10.1093/jmicro/dfz031","DOIUrl":"https://doi.org/10.1093/jmicro/dfz031","url":null,"abstract":"In this study, we investigated use of local surface plasmon resonance (LSPR) of metal nanoparticles (NPs) as a correlative light and electron microscopy (CLEM) tag for biological samples. Gold NPs in ultra-thin sections for TEM revealed that LSPR could be observed by optical microscopy at sizes of 20 nm or larger. Gold NPs at sizes less than 20 nm could be observed using the gold enhancement method. Therefore, this CLEM tag could be applied to immunoelectron microscopy using this gold enhancement method.","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2019-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfz031","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49134743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MicroscopyPub Date : 2019-12-03DOI: 10.1093/jmicro/dfz030
Kouji Inoue, N. Kuroda, Tetsuji Sato
{"title":"Elastic fiber system evaluated in the digestive organ of rats.","authors":"Kouji Inoue, N. Kuroda, Tetsuji Sato","doi":"10.1093/jmicro/dfz030","DOIUrl":"https://doi.org/10.1093/jmicro/dfz030","url":null,"abstract":"According to our previous reports, the intraperiodontal elastic fiber system comprises oxytalan fibers, whereas all types of elastic system fibers are present in the gingiva. Much remains to be elucidated regarding the topographic development of the elastic fiber system that constitutes the walls of the digestive organs. This study aimed to examine the topographic development of the elastic fiber system in the periodontal tissue, oral cavity and digestive tract of rats at light- and electron microscopic levels. At embryonic day 20, in situ hybridization revealed the mRNA expression of tropoelastin in the putative gingival lamina propria but not in the dental follicle. At the postnatal stage, the masticatory mucous membrane of the gingiva and hard palate comprised three different types of elastic system fibers (oxytalan, elaunin and elastic fibers). Conversely, the elastic fiber system comprised elaunin and elastic fibers in other oral mucosae and the lining mucosae of digestive tract organs (the esophagus, stomach and small intestine). The findings of our study suggest that the elastic fiber system is mainly related to tissue resistance in the periodontal ligament and tissue elasticity in the oral mucosae without masticatory mucosae and the overlying mucosa of digestive tracts and both functions in the gingiva and hard palate, respectively. The appearance of elaunin fibers in the periodontium of rats aged 14 weeks suggests the expression of tropoelastin induced by mechanical stressors such as mastication. The intraperiodontal difference in the distribution of elaunin fibers suggests heterogeneity among fibroblasts constituting the periodontium.","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2019-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfz030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49533922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MicroscopyPub Date : 2019-11-18DOI: 10.1093/jmicro/dfz038
He Zheng, Fan Cao, Ligong Zhao, Renhui Jiang, Peili Zhao, Ying Zhang, Yanjie Wei, S. Meng, Kaixuan Li, Shuangfeng Jia, Luying Li, Jianbo Wang
{"title":"Atomistic and dynamic structural characterizations in low-dimensional materials: recent applications of in situ transmission electron microscopy.","authors":"He Zheng, Fan Cao, Ligong Zhao, Renhui Jiang, Peili Zhao, Ying Zhang, Yanjie Wei, S. Meng, Kaixuan Li, Shuangfeng Jia, Luying Li, Jianbo Wang","doi":"10.1093/jmicro/dfz038","DOIUrl":"https://doi.org/10.1093/jmicro/dfz038","url":null,"abstract":"In situ transmission electron microscopy has achieved remarkable advances for atomic-scale dynamic analysis in low-dimensional materials and become an indispensable tool in view of linking a material's microstructure to its properties and performance. Here, accompanied with some cutting-edge researches worldwide, we briefly review our recent progress in dynamic atomistic characterization of low-dimensional materials under external mechanical stress, thermal excitations and electrical field. The electron beam irradiation effects in metals and metal oxides are also discussed. We conclude by discussing the likely future developments in this area.","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2019-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfz038","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45190772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MicroscopyPub Date : 2019-11-11DOI: 10.1093/jmicro/dfz035
T. Kodama, T. Kawasaki, T. Ikuta
{"title":"Properties of electrostatic correcting systems with annular apertures.","authors":"T. Kodama, T. Kawasaki, T. Ikuta","doi":"10.1093/jmicro/dfz035","DOIUrl":"https://doi.org/10.1093/jmicro/dfz035","url":null,"abstract":"Image formation in electron microscopes with circular hole and annular apertures is studied theoretically. The apertures-the circular hole aperture being negative with respect to the annular aperture-produce an additional electrostatic field that exerts a force on the electrons directed toward the optical axis. The resulting deflection angle decreases with increasing distance from the optical axis. This electrostatic field results in a correcting effect of the unavoidable spherical aberration of round electron lenses; the deflection toward the optical axis increases stronger than linearly with increasing distance from the optical axis. Analytical formulae are given for the correcting effect of circular hole and annular apertures. The expressions are based on the Davisson-Calbick formula, which is used to calculate focal length of a simple electrostatic lens.","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2019-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfz035","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48846189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MicroscopyPub Date : 2019-11-06DOI: 10.1093/jmicro/dfz032
Marina R. Mulenos, B. Zechmann, C. Sayes
{"title":"Sample preparation utilizing sputter coating increases contrast of cellulose nanocrystals in the transmission electron microscope.","authors":"Marina R. Mulenos, B. Zechmann, C. Sayes","doi":"10.1093/jmicro/dfz032","DOIUrl":"https://doi.org/10.1093/jmicro/dfz032","url":null,"abstract":"Cellulose nanocrystals (CNCs) are prepared for transmission electron microscopy (TEM) using positive or negative stains in an effort to increase the contrast between the specimen and background. When imaging CNCs, conventional stains have been shown to induce particle aggregation and produce artifacts. In this study, we report on methods used to image CNCs. To increase contrast and decrease artifacts and aggregation, sputter coating was used to coat the samples. CNCs were loaded onto copper grids and sputter coated with one of four different metals: iridium, carbon, gold, and titanium. The final layer was deposited at 5 nm to ensure surface homogeneity. The thin layer of conductive metal atoms deposited onto the specimen surface significantly increased contrast and improved image quality. The results presented here demonstrate the advantages of using sputter coating for imaging of highly crystalline cellulose materials with TEM.","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2019-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfz032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42073766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MicroscopyPub Date : 2019-11-01DOI: 10.1093/jmicro/dfz078
Y. Mineyuki, D. Yamauchi, T. Nakai, Daisuke Tamaoki, K. Uesugi, M. Hoshino, I. Karahara
{"title":"PB-12In Vivo Time-lapse Imaging of Changes in Air Space Distribution during Seed Imbibition in Lotus miyakojimae using X-ray Micro-CT","authors":"Y. Mineyuki, D. Yamauchi, T. Nakai, Daisuke Tamaoki, K. Uesugi, M. Hoshino, I. Karahara","doi":"10.1093/jmicro/dfz078","DOIUrl":"https://doi.org/10.1093/jmicro/dfz078","url":null,"abstract":"","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfz078","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45363536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MicroscopyPub Date : 2019-11-01DOI: 10.1093/jmicro/dfz088
T. Ito, Yohei K. Sato, M. Terauchi
{"title":"PM-05 Anisotropic Electronic Structure of Metal Borides Studied by Soft X-ray Emission Spectroscopy","authors":"T. Ito, Yohei K. Sato, M. Terauchi","doi":"10.1093/jmicro/dfz088","DOIUrl":"https://doi.org/10.1093/jmicro/dfz088","url":null,"abstract":"","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfz088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49161875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MicroscopyPub Date : 2019-11-01DOI: 10.1093/jmicro/dfz049
K. Shiroguchi
{"title":"SB-3 Combining live imaging and single-cell whole gene expression analysis by developing an automated cell picking system","authors":"K. Shiroguchi","doi":"10.1093/jmicro/dfz049","DOIUrl":"https://doi.org/10.1093/jmicro/dfz049","url":null,"abstract":"","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfz049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48978708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MicroscopyPub Date : 2019-11-01DOI: 10.1093/jmicro/dfz081
T. Tachibana, Hiroko Igarashi-Takeuchi, Y. Numabe
{"title":"PB-14 Effect of smoking inflammatory response to smoking cessation on human gingival fibroblast and periodontal ligaments cells","authors":"T. Tachibana, Hiroko Igarashi-Takeuchi, Y. Numabe","doi":"10.1093/jmicro/dfz081","DOIUrl":"https://doi.org/10.1093/jmicro/dfz081","url":null,"abstract":"","PeriodicalId":48655,"journal":{"name":"Microscopy","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfz081","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47635091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}