Detection of Autophagy-Related Structures in Fruiting Bodies of Edible Mushroom, Pleurotus ostreatus.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-04-21 DOI:10.1093/jmicro/dfac020

Yuma Ozaki, T. Aimi, N. Shimomura

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引用次数: 4

Abstract

Autophagy is involved in various fungal morphogenetic processes. However, there are limited reports regarding the role of autophagy in mushroom fruiting body formation. The purpose of this study was to reveal the autophagy-related structures in mushroom-forming fungi. The edible mushroom Pleurotus ostreatus was used in this study. Transmission electron microscopy revealed double-membrane bounded structures containing cytoplasmic components in the fruiting bodies of this fungus. Some of these double-membrane structures were observed to interact with the vacuoles. Additionally, curved flat cisternae of various lengths were detected in the cytoplasm. The shape, size, and thickness of the limiting membrane of the double-membrane structures and the flat cisternae corresponded well with those of the autophagosomes and the isolation membranes, respectively. Regarding autophagosome formation, a membrane-bound specific zone was detected near the isolation membrane, which appeared to expand along the novel membrane. This is the first detailed report showing autophagy-related structures in P. ostreatus and provides a possible model for autophagosome formation in these filamentous fungi. Mini-abstract Autophagy is involved in fungal morphogenetic processes. The fruiting bodies of edible mushroom Pleurotus ostreatus was observed under a TEM. The present study showed autophagy-related structures in this fungus and provides a possible model for autophagosome formation in filamentous fungi.

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食用菌平菇子实体中自噬相关结构的检测。

自噬参与了各种真菌的形态发生过程。然而，关于自噬在蘑菇子实体形成中的作用的报道有限。本研究的目的是揭示蘑菇形成真菌中与自噬相关的结构。本研究以食用菌平菇为材料。透射电子显微镜显示这种真菌的子实体中含有细胞质成分的双膜结合结构。观察到这些双膜结构中的一些与液泡相互作用。此外，在细胞质中检测到不同长度的弯曲扁平池。双膜结构和扁平池的限制膜的形状、大小和厚度分别与自噬体和分离膜的形状和厚度一致。关于自噬体的形成，在分离膜附近检测到一个膜结合特异性区，该区似乎沿着新膜扩展。这是第一份显示平菇自噬相关结构的详细报告，并为这些丝状真菌的自噬体形成提供了一个可能的模型。自噬参与真菌的形态发生过程。用透射电镜观察了平菇的子实体。本研究显示了这种真菌中与自噬相关的结构，并为丝状真菌中自噬体的形成提供了一个可能的模型。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.