Zoological Research最新文献

{"title":"Applications of single-cell RNA sequencing in spermatogenesis and molecular evolution.","authors":"Wen-Bo Chen, Meng-Fei Zhang, Fan Yang, Jin-Lian Hua","doi":"10.24272/j.issn.2095-8137.2024.010","DOIUrl":"10.24272/j.issn.2095-8137.2024.010","url":null,"abstract":"<p><p>Spermatogenic cell heterogeneity is determined by the complex process of spermatogenesis differentiation. However, effectively revealing the regulatory mechanisms underlying mammalian spermatogenic cell development and differentiation via traditional methods is difficult. Advances in technology have led to the emergence of many single-cell transcriptome sequencing protocols, which have partially addressed these challenges. In this review, we detail the principles of 10x Genomics technology and summarize the methods for downstream analysis of single-cell transcriptome sequencing data. Furthermore, we explore the role of single-cell transcriptome sequencing in revealing the heterogeneity of testicular ecological niche cells, delineating the establishment and disruption of testicular immune homeostasis during human spermatogenesis, investigating abnormal spermatogenesis in humans, and, ultimately, elucidating the molecular evolution of mammalian spermatogenesis.</p>","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"45 3","pages":"575-585"},"PeriodicalIF":4.0,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11188606/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141065230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring cerebral structural and functional abnormalities in a mouse model of post-traumatic headache induced by mild traumatic brain injury.","authors":"Dan Yang, Bin-Bin Nie, Jin-Gang He, Zong-Qiang Lv, Feng-Feng Mo, Si-Yi Ouyang, Jie Wang, Juxiang Chen, Tao Tao","doi":"10.24272/j.issn.2095-8137.2023.323","DOIUrl":"10.24272/j.issn.2095-8137.2023.323","url":null,"abstract":"<p><p>Mild traumatic brain injury (mTBI)-induced post-traumatic headache (PTH) is a pressing public health concern and leading cause of disability worldwide. Although PTH is often accompanied by neurological disorders, the exact underlying mechanism remains largely unknown. Identifying potential biomarkers may prompt the diagnosis and development of effective treatments for mTBI-induced PTH. In this study, a mouse model of mTBI-induced PTH was established to investigate its effects on cerebral structure and function during short-term recovery. Results indicated that mice with mTBI-induced PTH exhibited balance deficits during the early post-injury stage. Metabolic kinetics revealed that variations in neurotransmitters were most prominent in the cerebellum, temporal lobe/cortex, and hippocampal regions during the early stages of PTH. Additionally, variations in brain functional activities and connectivity were further detected in the early stage of PTH, particularly in the cerebellum and temporal cortex, suggesting that these regions play central roles in the mechanism underlying PTH. Moreover, our results suggested that GABA and glutamate may serve as potential diagnostic or prognostic biomarkers for PTH. Future studies should explore the specific neural circuits involved in the regulation of PTH by the cerebellum and temporal cortex, with these two regions potentially utilized as targets for non-invasive stimulation in future clinical treatment.</p>","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"45 3","pages":"648-662"},"PeriodicalIF":4.0,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11188605/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141065499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of wild-type HRAS drives non-alcoholic steatohepatitis to hepatocellular carcinoma in mice.","authors":"Chen Ling, Su-Su Liu, Yu-Ya Wang, Gui-Tao Huo, Yan-Wei Yang, Nan Xu, Hong Wang, Yong Wu, Yu-Fa Miao, Rui Fu, Yu-Wei Zhao, Chang-Fa Fan","doi":"10.24272/j.issn.2095-8137.2024.002","DOIUrl":"10.24272/j.issn.2095-8137.2024.002","url":null,"abstract":"<p><p>Hepatocellular carcinoma (HCC), a prevalent solid carcinoma of significant concern, is an aggressive and often fatal disease with increasing global incidence rates and poor therapeutic outcomes. The etiology and pathological progression of non-alcoholic steatohepatitis (NASH)-related HCC is multifactorial and multistage. However, no single animal model can accurately mimic the full NASH-related HCC pathological progression, posing considerable challenges to transition and mechanistic studies. Herein, a novel conditional inducible wild-type human <i>HRAS</i> overexpressed mouse model (HRAS-HCC) was established, demonstrating 100% morbidity and mortality within approximately one month under normal dietary and lifestyle conditions. Advanced symptoms of HCC such as ascites, thrombus, internal hemorrhage, jaundice, and lung metastasis were successfully replicated in mice. In-depth pathological features of NASH- related HCC were demonstrated by pathological staining, biochemical analyses, and typical marker gene detections. Combined murine anti-PD-1 and sorafenib treatment effectively prolonged mouse survival, further confirming the accuracy and reliability of the model. Based on protein-protein interaction (PPI) network and RNA sequencing analyses, we speculated that overexpression of HRAS may initiate the THBS1-COL4A3 axis to induce NASH with severe fibrosis, with subsequent progression to HCC. Collectively, our study successfully duplicated natural sequential progression in a single murine model over a very short period, providing an accurate and reliable preclinical tool for therapeutic evaluations targeting the NASH to HCC continuum.</p>","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"45 3","pages":"551-566"},"PeriodicalIF":4.0,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11188599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140959782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The neuroscience of pain, addiction, and anesthesia.","authors":"Ji Hu, Ping Zheng","doi":"10.24272/j.issn.2095-8137.2024.138","DOIUrl":"10.24272/j.issn.2095-8137.2024.138","url":null,"abstract":"","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"45 3","pages":"709-710"},"PeriodicalIF":4.0,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11188610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141065603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated multi-omics analysis reveals liver metabolic reprogramming by fish iridovirus and antiviral function of alpha-linolenic acid.","authors":"Lin Liu, Ya Zhang, Meng-Di Yuan, Dong-Miao Xiao, Wei-Hua Xu, Qi Zheng, Qi-Wei Qin, You-Hua Huang, Xiao-Hong Huang","doi":"10.24272/j.issn.2095-8137.2024.028","DOIUrl":"10.24272/j.issn.2095-8137.2024.028","url":null,"abstract":"<p><p>Iridovirus poses a substantial threat to global aquaculture due to its high mortality rate; however, the molecular mechanisms underpinning its pathogenesis are not well elucidated. Here, a multi-omics approach was applied to groupers infected with Singapore grouper iridovirus (SGIV), focusing on the roles of key metabolites. Results showed that SGIV induced obvious histopathological damage and changes in metabolic enzymes within the liver. Furthermore, SGIV significantly reduced the contents of lipid droplets, triglycerides, cholesterol, and lipoproteins. Metabolomic analysis indicated that the altered metabolites were enriched in 19 pathways, with a notable down-regulation of lipid metabolites such as glycerophosphates and alpha-linolenic acid (ALA), consistent with disturbed lipid homeostasis in the liver. Integration of transcriptomic and metabolomic data revealed that the top enriched pathways were related to cell growth and death and nucleotide, carbohydrate, amino acid, and lipid metabolism, supporting the conclusion that SGIV infection induced liver metabolic reprogramming. Further integrative transcriptomic and proteomic analysis indicated that SGIV infection activated crucial molecular events in a phagosome-immune depression-metabolism dysregulation-necrosis signaling cascade. Of note, integrative multi-omics analysis demonstrated the consumption of ALA and linoleic acid (LA) metabolites, and the accumulation of L-glutamic acid (GA), accompanied by alterations in immune, inflammation, and cell death-related genes. Further experimental data showed that ALA, but not GA, suppressed SGIV replication by activating antioxidant and anti-inflammatory responses in the host. Collectively, these findings provide a comprehensive resource for understanding host response dynamics during fish iridovirus infection and highlight the antiviral potential of ALA in the prevention and treatment of iridoviral diseases.</p>","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"45 3","pages":"520-534"},"PeriodicalIF":4.0,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11188608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140873127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial targeting sequence of magnetoreceptor MagR: More than just targeting.","authors":"Yanqi Zhang, Peng Zhang, Junjun Wang, Jing Zhang, Tianyang Tong, Xiujuan Zhou, Yajie Zhou, Mengke Wei, Chuanlin Feng, Jinqian Li, Xin Zhang, Can Xie, Tiantian Cai","doi":"10.24272/j.issn.2095-8137.2023.385","DOIUrl":"10.24272/j.issn.2095-8137.2023.385","url":null,"abstract":"<p><p>Iron-sulfur clusters are essential cofactors for proteins involved in various biological processes, such as electron transport, biosynthetic reactions, DNA repair, and gene expression regulation. Iron-sulfur cluster assembly protein IscA1 (or MagR) is found within the mitochondria of most eukaryotes. Magnetoreceptor (MagR) is a highly conserved A-type iron and iron-sulfur cluster-binding protein, characterized by two distinct types of iron-sulfur clusters, [2Fe-2S] and [3Fe-4S], each conferring unique magnetic properties. MagR forms a rod-like polymer structure in complex with photoreceptive cryptochrome (Cry) and serves as a putative magnetoreceptor for retrieving geomagnetic information in animal navigation. Although the N-terminal sequences of MagR vary among species, their specific function remains unknown. In the present study, we found that the N-terminal sequences of pigeon MagR, previously thought to serve as a mitochondrial targeting signal (MTS), were not cleaved following mitochondrial entry but instead modulated the efficiency with which iron-sulfur clusters and irons are bound. Moreover, the N-terminal region of MagR was required for the formation of a stable MagR/Cry complex. Thus, the N-terminal sequences in pigeon MagR fulfil more important functional roles than just mitochondrial targeting. These results further extend our understanding of the function of MagR and provide new insights into the origin of magnetoreception from an evolutionary perspective.</p>","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"45 3","pages":"468-477"},"PeriodicalIF":4.0,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11188603/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140867164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement in Tol2 transposon for efficient large-cargo capacity transgene applications in cultured cells and zebrafish ( <i>Danio rerio</i>).","authors":"Peng-Cheng Wang, Hao Deng, Rang Xu, Jiu-Lin Du, Rongkun Tao","doi":"10.24272/j.issn.2095-8137.2024.026","DOIUrl":"10.24272/j.issn.2095-8137.2024.026","url":null,"abstract":"<p><p>Most viruses and transposons serve as effective carriers for the introduction of foreign DNA up to 11 kb into vertebrate genomes. However, their activity markedly diminishes with payloads exceeding 11 kb. Expanding the payload capacity of transposons could facilitate more sophisticated cargo designs, improving the regulation of expression and minimizing mutagenic risks associated with molecular therapeutics, metabolic engineering, and transgenic animal production. In this study, we improved the Tol2 transposon by increasing protein expression levels using a translational enhancer ( <i>QBI SP163</i>, ST) and enhanced the nuclear targeting ability using the nuclear localization protein H2B (SHT). The modified Tol2 and ST transposon efficiently integrated large DNA cargos into human cell cultures (H1299), comparable to the well-established super PiggyBac system. Furthermore, mRNA from ST and SHT showed a significant increase in transgene delivery efficiency of large DNA payloads (8 kb, 14 kb, and 24 kb) into zebrafish ( <i>Danio rerio</i>). This study presents a modified Tol2 transposon as an enhanced nonviral vector for the delivery of large DNA payloads in transgenic applications.</p>","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"45 3","pages":"567-574"},"PeriodicalIF":4.0,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11188598/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140959852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Next-generation vaccines for substance use disorders.","authors":"Kaixuan Wang, Hongshuang Wang, Xiaohui Wang","doi":"10.24272/j.issn.2095-8137.2023.407","DOIUrl":"10.24272/j.issn.2095-8137.2023.407","url":null,"abstract":"","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"45 3","pages":"707-708"},"PeriodicalIF":4.0,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11188595/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141065581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing neuroscience through real-time processing of big data: Transition from open-loop to closed-loop paradigms.","authors":"Yu-Fan Wang, Jiu-Lin Du","doi":"10.24272/j.issn.2095-8137.2024.099","DOIUrl":"10.24272/j.issn.2095-8137.2024.099","url":null,"abstract":"","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"45 3","pages":"518-519"},"PeriodicalIF":4.0,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11188594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140858654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Enterococcus</i> <i>faecalis</i> provides protection during scavenging in carrion crow ( <i>Corvus</i> <i>corone</i>).","authors":"Bin Hu, Jia-Min Wang, Qing-Xun Zhang, Jing Xu, Ya-Nan Xing, Bo Wang, Shu-Yi Han, Hong-Xuan He","doi":"10.24272/j.issn.2095-8137.2023.320","DOIUrl":"10.24272/j.issn.2095-8137.2023.320","url":null,"abstract":"<p><p>The gut microbiota significantly influences host physiology and provides essential ecosystem services. While diet can affect the composition of the gut microbiota, the gut microbiota can also help the host adapt to specific dietary habits. The carrion crow ( <i>Corvus</i> <i>corone</i>), an urban facultative scavenger bird, hosts an abundance of pathogens due to its scavenging behavior. Despite this, carrion crows infrequently exhibit illness, a phenomenon related to their unique physiological adaptability. At present, however, the role of the gut microbiota remains incompletely understood. In this study, we performed a comparative analysis using 16S rRNA amplicon sequencing technology to assess colonic content in carrion crows and 16 other bird species with different diets in Beijing, China. Our findings revealed that the dominant gut microbiota in carrion crows was primarily composed of Proteobacteria (75.51%) and Firmicutes (22.37%). Significant differences were observed in the relative abundance of <i>Enterococcus</i> <i>faecalis</i> among groups, highlighting its potential as a biomarker of facultative scavenging behavior in carrion crows. Subsequently, <i>E.</i> <i>faecalis</i> isolated from carrion crows was transplanted into model mice to explore the protective effects of this bacterial community against <i>Salmonella enterica</i> infection. Results showed that <i>E.</i> <i>faecalis</i> down-regulated the expression of pro-inflammatory cytokines tumor necrosis factor alpha (TNF-α), interferon gamma (IFN-γ), and interleukin 6 (IL-6), prevented <i>S. enterica</i> colonization, and regulated the composition of gut microbiota in mice, thereby modulating the host's immune regulatory capacity. Therefore, <i>E. faecalis</i> exerts immunoregulatory and anti-pathogenic functions in carrion crows engaged in scavenging behavior, offering a representative case of how the gut microbiota contributes to the protection of hosts with specialized diets.</p>","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"45 3","pages":"451-463"},"PeriodicalIF":4.0,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11188602/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140860209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}