Zoological Research最新文献_第7页

Human-like adrenal features in Chinese tree shrews revealed by multi-omics analysis of adrenal cell populations and steroid synthesis. 通过肾上腺细胞群和类固醇合成的多组学分析揭示中国树鼩的类人肾上腺特征

IF 4 1区生物学

Zoological Research Pub Date : 2024-05-18 DOI: 10.24272/j.issn.2095-8137.2023.280

Jing-Hang Jiang, Yi-Fu Wang, Jie Zheng, Yi-Ming Lei, Zhong-Yuan Chen, Yi Guo, Ya-Jie Guo, Bing-Qian Guo, Yu-Fang Lv, Hong-Hong Wang, Juan-Juan Xie, Yi-Xuan Liu, Ting-Wei Jin, Bi-Qi Li, Xiao-Shu Zhu, Yong-Hua Jiang, Zeng-Nan Mo

{"title":"Human-like adrenal features in Chinese tree shrews revealed by multi-omics analysis of adrenal cell populations and steroid synthesis.","authors":"Jing-Hang Jiang, Yi-Fu Wang, Jie Zheng, Yi-Ming Lei, Zhong-Yuan Chen, Yi Guo, Ya-Jie Guo, Bing-Qian Guo, Yu-Fang Lv, Hong-Hong Wang, Juan-Juan Xie, Yi-Xuan Liu, Ting-Wei Jin, Bi-Qi Li, Xiao-Shu Zhu, Yong-Hua Jiang, Zeng-Nan Mo","doi":"10.24272/j.issn.2095-8137.2023.280","DOIUrl":"10.24272/j.issn.2095-8137.2023.280","url":null,"abstract":"The Chinese tree shrew ( Tupaia belangeri chinensis) has emerged as a promising model for investigating adrenal steroid synthesis, but it is unclear whether the same cells produce steroid hormones and whether their production is regulated in the same way as in humans. Here, we comprehensively mapped the cell types and pathways of steroid metabolism in the adrenal gland of Chinese tree shrews using single-cell RNA sequencing, spatial transcriptome analysis, mass spectrometry, and immunohistochemistry. We compared the transcriptomes of various adrenal cell types across tree shrews, humans, macaques, and mice. Results showed that tree shrew adrenal glands expressed many of the same key enzymes for steroid synthesis as humans, including CYP11B2, CYP11B1, CYB5A, and CHGA. Biochemical analysis confirmed the production of aldosterone, cortisol, and dehydroepiandrosterone but not dehydroepiandrosterone sulfate in the tree shrew adrenal glands. Furthermore, genes in adrenal cell types in tree shrews were correlated with genetic risk factors for polycystic ovary syndrome, primary aldosteronism, hypertension, and related disorders in humans based on genome-wide association studies. Overall, this study suggests that the adrenal glands of Chinese tree shrews may consist of closely related cell populations with functional similarity to those of the human adrenal gland. Our comprehensive results (publicly available at http://gxmujyzmolab.cn:16245/scAGMap/) should facilitate the advancement of this animal model for the investigation of adrenal gland disorders.","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"45 3","pages":"617-632"},"PeriodicalIF":4.0,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11188597/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141065575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microglia in drug addiction: A perspective from neuroimmunopharmacology. 药物成瘾中的小胶质细胞：神经免疫药理学的视角。

IF 4 1区生物学

Zoological Research Pub Date : 2024-05-18 DOI: 10.24272/j.issn.2095-8137.2023.286

Cong Lin, Xiaohui Wang

引用次数: 0

Identification of novel mammalian viruses in tree shrews ( Tupaia belangeri chinensis). 鉴定树鼩（Tupaia belangeri chinensis）体内的新型哺乳动物病毒。

IF 4.9 1区生物学

Zoological Research Pub Date : 2024-03-18 DOI: 10.24272/j.issn.2095-8137.2023.306

Hong Zhou, Ren-Rong Tian, Xiu-Rong Wang, Jin-Xuan Yang, Yun-Xiao Wang, Ming-Liang Zhao, Xu-Dong Zhang, Yu-Hua Ma, Long-Bao Lv, Edward C Holmes, Yong-Tang Zheng, Wei-Feng Shi

{"title":"Identification of novel mammalian viruses in tree shrews ( Tupaia belangeri chinensis).","authors":"Hong Zhou, Ren-Rong Tian, Xiu-Rong Wang, Jin-Xuan Yang, Yun-Xiao Wang, Ming-Liang Zhao, Xu-Dong Zhang, Yu-Hua Ma, Long-Bao Lv, Edward C Holmes, Yong-Tang Zheng, Wei-Feng Shi","doi":"10.24272/j.issn.2095-8137.2023.306","DOIUrl":"10.24272/j.issn.2095-8137.2023.306","url":null,"abstract":"The Chinese tree shrew ( Tupaia belangeri chinensis), a member of the mammalian order Scandentia, exhibits considerable similarities with primates, including humans, in aspects of its nervous, immune, and metabolic systems. These similarities have established the tree shrew as a promising experimental model for biomedical research on cancer, infectious diseases, metabolic disorders, and mental health conditions. Herein, we used meta-transcriptomic sequencing to analyze plasma, as well as oral and anal swab samples, from 105 healthy asymptomatic tree shrews to identify the presence of potential zoonotic viruses. In total, eight mammalian viruses with complete genomes were identified, belonging to six viral families, including Flaviviridae, Hepeviridae, Parvovirinae, Picornaviridae, Sedoreoviridae, and Spinareoviridae. Notably, the presence of rotavirus was recorded in tree shrews for the first time. Three viruses - hepacivirus 1, parvovirus, and picornavirus - exhibited low genetic similarity (<70%) with previously reported viruses at the whole-genome scale, indicating novelty. Conversely, three other viruses - hepacivirus 2, hepatovirus A and hepevirus - exhibited high similarity (>94%) to known viral strains. Phylogenetic analyses also revealed that the rotavirus and mammalian orthoreovirus identified in this study may be novel reassortants. These findings provide insights into the diverse viral spectrum present in captive Chinese tree shrews, highlighting the necessity for further research into their potential for cross-species transmission.","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"45 2","pages":"429-438"},"PeriodicalIF":4.9,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11017089/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140132957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and effective regulation of scarb1 gene involved in pigmentation change in autotetraploid Carassius auratus. 参与自交系鲫鱼色素变化的 scarb1 基因的鉴定和有效调控

IF 4.9 1区生物学

Zoological Research Pub Date : 2024-03-18 DOI: 10.24272/j.issn.2095-8137.2023.293

Xi-Dan Xu, Yue Zhou, Chong-Qing Wang, Xu Huang, Kun Zhang, Xiao-Wei Xu, Li-Wen He, Xin-Yue Zhang, Xin-Zhu Fu, Ming Ma, Qin-Bo Qin, Shao-Jun Liu

{"title":"Identification and effective regulation of scarb1 gene involved in pigmentation change in autotetraploid Carassius auratus.","authors":"Xi-Dan Xu, Yue Zhou, Chong-Qing Wang, Xu Huang, Kun Zhang, Xiao-Wei Xu, Li-Wen He, Xin-Yue Zhang, Xin-Zhu Fu, Ming Ma, Qin-Bo Qin, Shao-Jun Liu","doi":"10.24272/j.issn.2095-8137.2023.293","DOIUrl":"10.24272/j.issn.2095-8137.2023.293","url":null,"abstract":"The autotetraploid Carassius auratus (4nRR, 4 n=200, RRRR) is derived from whole-genome duplication of Carassius auratus red var. (RCC, 2 n=100, RR). In the current study, we demonstrated that chromatophores and pigment changes directly caused the coloration and variation of 4nRR skin (red in RCC, brownish-yellow in 4nRR). To further explore the molecular mechanisms underlying coloration formation and variation in 4nRR, we performed transcriptome profiling and molecular functional verification in RCC and 4nRR. Results revealed that scarb1, associated with carotenoid metabolism, underwent significant down-regulation in 4nRR. Efficient editing of this candidate pigment gene provided clear evidence of its significant role in RCC coloration. Subsequently, we identified four divergent scarb1 homeologs in 4nRR: two original scarb1 homeologs from RCC and two duplicated ones. Notably, three of these homeologs possessed two highly conserved alleles, exhibiting biased and allele-specific expression in the skin. Remarkably, after precise editing of both the original and duplicated scarb1 homeologs and/or alleles, 4nRR individuals, whether singly or multiply mutated, displayed a transition from brownish-yellow skin to a cyan-gray phenotype. Concurrently, the proportional areas of the cyan-gray regions displayed a gene-dose correlation. These findings illustrate the subfunctionalization of duplicated scarb1, with all scarb1 genes synergistically and equally contributing to the pigmentation of 4nRR. This is the first report concerning the functional differentiation of duplicated homeologs in an autopolyploid fish, substantially enriching our understanding of coloration formation and change within this group of organisms.","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"45 2","pages":"381-397"},"PeriodicalIF":4.9,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11017083/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140132956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporal dynamics of the social structure of Indo-Pacific humpback dolphins ( Sousa chinensis) in Xiamen waters from 2007 to 2019. 2007-2019年厦门海域印度洋中华白海豚社会结构的时空动态变化。

IF 4.9 1区生物学

Zoological Research Pub Date : 2024-03-18 DOI: 10.24272/j.issn.2095-8137.2023.255

Yi Lu, Xin-Rong Xu, Bing-Yao Chen, Thomas A Jefferson, Holly Fearnbach, Guang Yang

{"title":"Spatiotemporal dynamics of the social structure of Indo-Pacific humpback dolphins ( Sousa chinensis) in Xiamen waters from 2007 to 2019.","authors":"Yi Lu, Xin-Rong Xu, Bing-Yao Chen, Thomas A Jefferson, Holly Fearnbach, Guang Yang","doi":"10.24272/j.issn.2095-8137.2023.255","DOIUrl":"10.24272/j.issn.2095-8137.2023.255","url":null,"abstract":"As highly social animals, Indo-Pacific humpback dolphins ( Sousa chinensis) exhibit community differentiation. Nevertheless, our understanding of the external and internal factors influencing these dynamics, as well as their spatiotemporal variations, is still limited. In the present study, variations in the social structure of an endangered Indo-Pacific humpback dolphin population in Xiamen Bay, China, were monitored over two distinct periods (2007-2010 and 2017-2019) to analyze the effects of habitat utilization and the composition of individuals within the population. In both periods, the population demonstrated a strikingly similar pattern of social differentiation, characterized by the division of individuals into two main clusters and one small cluster. Spatially, the two primary clusters occupied the eastern and western waters, respectively, although the core distribution area of the eastern cluster shifted further eastward between the two periods. Despite this distribution shift, the temporal stability of the social structure and inter-associations within the eastern cluster remained unaffected. A subset of 16 individuals observed in both periods, comprising 51.6% and 43.2% of the population in each respective period, emerged as a foundational element of the social structure and may be responsible for sustaining social structure stability, especially during the 2007-2010 period. These observations suggest that the composition of dominant individuals, an internal factor, had a more substantial influence on the formation of the social network than changes in habitat use, an external factor. Consequently, the study proposes distinct conservation measures tailored to each of the two main clusters.","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"45 2","pages":"439-450"},"PeriodicalIF":4.9,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11017072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140132880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA m6A dynamic modification mediated by nucleus-localized FTO is involved in follicular reserve. 核定位 FTO 介导的 RNA m6A 动态修饰参与了卵泡储备。

IF 4.9 1区生物学

Zoological Research Pub Date : 2024-03-18 DOI: 10.24272/j.issn.2095-8137.2023.236

Jiao-Na Zhang, Rui-Ting Wang, Francesca-Gioia Klinger, Shun-Feng Cheng, Wei Shen, Xiao-Feng Sun

{"title":"RNA m6A dynamic modification mediated by nucleus-localized FTO is involved in follicular reserve.","authors":"Jiao-Na Zhang, Rui-Ting Wang, Francesca-Gioia Klinger, Shun-Feng Cheng, Wei Shen, Xiao-Feng Sun","doi":"10.24272/j.issn.2095-8137.2023.236","DOIUrl":"10.24272/j.issn.2095-8137.2023.236","url":null,"abstract":"In eukaryotic organisms, the most common internal modification of messenger RNA (mRNA) is N6-methyladenosine (m6A). This modification can be dynamically and reversibly controlled by specific enzymes known as m6A writers and erasers. The fat-mass and obesity-associated protein (FTO) catalyzes RNA demethylation and plays a critical role in various physiological and pathological processes. Our research identified dynamic alterations in both m6A and FTO during the assembly of primordial follicles, with an inverse relationship observed for m6A levels and nuclear-localized FTO expression. Application of Fto small interfering RNA (siRNA) altered the expression of genes related to cell proliferation, hormone regulation, and cell chemotaxis, and affected RNA alternative splicing. Overexpression of the full-length Fto gene led to changes in m6A levels, alternative splicing of Cdk5, cell proliferation, cell cycle progression, and proportion of primordial follicles. Conversely, overexpression of Fto lacking a nuclear localization signal (NLS) did not significantly alter m6A levels or primordial follicle assembly. These findings suggest that FTO, localized in the nucleus but not in the cytoplasm, regulates RNA m6A demethylation and plays a role in cell proliferation, cell cycle progression, and primordial follicle assembly. These results highlight the potential of m6A and its eraser FTO as possible biomarkers and therapeutic targets.","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"45 2","pages":"415-428"},"PeriodicalIF":4.9,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11017081/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140132960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetically modified non-human primate models for research on neurodegenerative diseases. 用于神经退行性疾病研究的转基因非人灵长类动物模型。

IF 4.9 1区生物学

Zoological Research Pub Date : 2024-03-18 DOI: 10.24272/j.issn.2095-8137.2023.197

Ming-Tian Pan, Han Zhang, Xiao-Jiang Li, Xiang-Yu Guo

{"title":"Genetically modified non-human primate models for research on neurodegenerative diseases.","authors":"Ming-Tian Pan, Han Zhang, Xiao-Jiang Li, Xiang-Yu Guo","doi":"10.24272/j.issn.2095-8137.2023.197","DOIUrl":"10.24272/j.issn.2095-8137.2023.197","url":null,"abstract":"Neurodegenerative diseases (NDs) are a group of debilitating neurological disorders that primarily affect elderly populations and include Alzheimer's disease (AD), Parkinson's disease (PD), Huntington's disease (HD), and amyotrophic lateral sclerosis (ALS). Currently, there are no therapies available that can delay, stop, or reverse the pathological progression of NDs in clinical settings. As the population ages, NDs are imposing a huge burden on public health systems and affected families. Animal models are important tools for preclinical investigations to understand disease pathogenesis and test potential treatments. While numerous rodent models of NDs have been developed to enhance our understanding of disease mechanisms, the limited success of translating findings from animal models to clinical practice suggests that there is still a need to bridge this translation gap. Old World non-human primates (NHPs), such as rhesus, cynomolgus, and vervet monkeys, are phylogenetically, physiologically, biochemically, and behaviorally most relevant to humans. This is particularly evident in the similarity of the structure and function of their central nervous systems, rendering such species uniquely valuable for neuroscience research. Recently, the development of several genetically modified NHP models of NDs has successfully recapitulated key pathologies and revealed novel mechanisms. This review focuses on the efficacy of NHPs in modeling NDs and the novel pathological insights gained, as well as the challenges associated with the generation of such models and the complexities involved in their subsequent analysis.","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"45 2","pages":"263-274"},"PeriodicalIF":4.9,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11017080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139576778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of SHROOM3 affects neuroepithelial cell shape through regulating cytoskeleton proteins in cynomolgus monkey organoids. SHROOM3的缺失会通过调节猴器官组织中的细胞骨架蛋白影响神经上皮细胞的形状。

IF 4.9 1区生物学

Zoological Research Pub Date : 2024-03-18 DOI: 10.24272/j.issn.2095-8137.2023.190

Peng Li, Ting Zhang, Ruo Wu, Jun-Yu Zhang, Yan Zhuo, Shan-Gang Li, Jiao-Jian Wang, Wen-Ting Guo, Zheng-Bo Wang, Yong-Chang Chen

{"title":"Loss of SHROOM3 affects neuroepithelial cell shape through regulating cytoskeleton proteins in cynomolgus monkey organoids.","authors":"Peng Li, Ting Zhang, Ruo Wu, Jun-Yu Zhang, Yan Zhuo, Shan-Gang Li, Jiao-Jian Wang, Wen-Ting Guo, Zheng-Bo Wang, Yong-Chang Chen","doi":"10.24272/j.issn.2095-8137.2023.190","DOIUrl":"10.24272/j.issn.2095-8137.2023.190","url":null,"abstract":"Neural tube defects (NTDs) are severe congenital neurodevelopmental disorders arising from incomplete neural tube closure. Although folate supplementation has been shown to mitigate the incidence of NTDs, some cases, often attributable to genetic factors, remain unpreventable. The SHROOM3 gene has been implicated in NTD cases that are unresponsive to folate supplementation; at present, however, the underlying mechanism remains unclear. Neural tube morphogenesis is a complex process involving the folding of the planar epithelium of the neural plate. To determine the role of SHROOM3 in early developmental morphogenesis, we established a neuroepithelial organoid culture system derived from cynomolgus monkeys to closely mimic the in vivo neural plate phase. Loss of SHROOM3 resulted in shorter neuroepithelial cells and smaller nuclei. These morphological changes were attributed to the insufficient recruitment of cytoskeletal proteins, namely fibrous actin (F-actin), myosin II, and phospho-myosin light chain (PMLC), to the apical side of the neuroepithelial cells. Notably, these defects were not rescued by folate supplementation. RNA sequencing revealed that differentially expressed genes were enriched in biological processes associated with cellular and organ morphogenesis. In summary, we established an authentic in vitro system to study NTDs and identified a novel mechanism for NTDs that are unresponsive to folate supplementation.","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"45 2","pages":"233-241"},"PeriodicalIF":4.9,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11017078/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139576836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Androgen signaling inhibits de novo lipogenesis to alleviate lipid deposition in zebrafish. 雄性激素信号抑制新脂肪生成以减轻斑马鱼的脂质沉积。

IF 4.9 1区生物学

Zoological Research Pub Date : 2024-03-18 DOI: 10.24272/j.issn.2095-8137.2023.324

Jing-Yi Jia, Guang-Hui Chen, Ting-Ting Shu, Qi-Yong Lou, Xia Jin, Jiang-Yan He, Wu-Han Xiao, Gang Zhai, Zhan Yin

{"title":"Androgen signaling inhibits de novo lipogenesis to alleviate lipid deposition in zebrafish.","authors":"Jing-Yi Jia, Guang-Hui Chen, Ting-Ting Shu, Qi-Yong Lou, Xia Jin, Jiang-Yan He, Wu-Han Xiao, Gang Zhai, Zhan Yin","doi":"10.24272/j.issn.2095-8137.2023.324","DOIUrl":"10.24272/j.issn.2095-8137.2023.324","url":null,"abstract":"Testosterone is closely associated with lipid metabolism and known to affect body fat composition and muscle mass in males. However, the mechanisms by which testosterone acts on lipid metabolism are not yet fully understood, especially in teleosts. In this study, cyp17a1-/- zebrafish ( Danio rerio) exhibited excessive visceral adipose tissue (VAT), lipid content, and up-regulated expression and activity of hepatic de novo lipogenesis (DNL) enzymes. The assay for transposase accessible chromatin with sequencing (ATAC-seq) results demonstrated that chromatin accessibility of DNL genes was increased in cyp17a1-/- fish compared to cyp17a1+/+ male fish, including stearoyl-CoA desaturase ( scd) and fatty acid synthase ( fasn). Androgen response element (ARE) motifs in the androgen signaling pathway were significantly enriched in cyp17a1+/+ male fish but not in cyp17a1-/- fish. Both androgen receptor ( ar)-/- and wild-type (WT) zebrafish administered with Ar antagonist flutamide displayed excessive visceral adipose tissue, lipid content, and up-regulated expression and activity of hepatic de novo lipogenesis enzymes. The Ar agonist BMS-564929 reduced the content of VAT and lipid content, and down-regulated acetyl-CoA carboxylase a ( acaca), fasn, and scd expression. Mechanistically, the rescue effect of testosterone on cyp17a1-/- fish in terms of phenotypes was abolished when ar was additionally depleted. Collectively, these findings reveal that testosterone inhibits lipid deposition by down-regulating DNL genes via Ar in zebrafish, thus expanding our understanding of the relationship between testosterone and lipid metabolism in teleosts.","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"45 2","pages":"355-366"},"PeriodicalIF":4.9,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11017085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140132948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative transcriptome analysis between rhesus macaques ( Macaca mulatta) and crab-eating macaques ( M. fascicularis). 猕猴（Macaca mulatta）与食蟹猕猴（M. fascicularis）的转录组比较分析。

IF 4.9 1区生物学

Zoological Research Pub Date : 2024-03-18 DOI: 10.24272/j.issn.2095-8137.2023.322

Yu-Xiang Mao, Yamei Li, Zikun Yang, Ning Xu, Shilong Zhang, Xuankai Wang, Xiangyu Yang, Qiang Sun, Yafei Mao

{"title":"Comparative transcriptome analysis between rhesus macaques ( Macaca mulatta) and crab-eating macaques ( M. fascicularis).","authors":"Yu-Xiang Mao, Yamei Li, Zikun Yang, Ning Xu, Shilong Zhang, Xuankai Wang, Xiangyu Yang, Qiang Sun, Yafei Mao","doi":"10.24272/j.issn.2095-8137.2023.322","DOIUrl":"10.24272/j.issn.2095-8137.2023.322","url":null,"abstract":"Understanding gene expression variations between species is pivotal for deciphering the evolutionary diversity in phenotypes. Rhesus macaques ( Macaca mulatta, MMU) and crab-eating macaques ( M. fascicularis, MFA) serve as crucial nonhuman primate biomedical models with different phenotypes. To date, however, large-scale comparative transcriptome research between these two species has not yet been fully explored. Here, we conducted systematic comparisons utilizing newly sequenced RNA-seq data from 84 samples (41 MFA samples and 43 MMU samples) encompassing 14 common tissues. Our findings revealed a small fraction of genes (3.7%) with differential expression between the two species, as well as 36.5% of genes with tissue-specific expression in both macaques. Comparison of gene expression between macaques and humans indicated that 22.6% of orthologous genes displayed differential expression in at least two tissues. Moreover, 19.41% of genes that overlapped with macaque-specific structural variants showed differential expression between humans and macaques. Of these, the FAM220A gene exhibited elevated expression in humans compared to macaques due to lineage-specific duplication. In summary, this study presents a large-scale transcriptomic comparison between MMU and MFA and between macaques and humans. The discovery of gene expression variations not only enhances the biomedical utility of macaque models but also contributes to the wider field of primate genomics.","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"45 2","pages":"299-310"},"PeriodicalIF":4.9,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11017088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140132950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0