Zoological Research最新文献

{"title":"Elevated CXCL1 triggers dopaminergic neuronal loss in the substantia nigra of C57BL/6J mice: Evaluation of a novel Parkinsonian mouse model.","authors":"Xi-Zhen Ma, Guo-Rui Jia, Meng-Yu Li, Sheng-Han Zhang, Zhao-Xin Wang, Ning Song, Ying-Juan Liu, Jun-Xia Xie","doi":"10.24272/j.issn.2095-8137.2024.228","DOIUrl":"10.24272/j.issn.2095-8137.2024.228","url":null,"abstract":"<p><p>Substantial evidence points to the early onset of peripheral inflammation in the development of Parkinson's disease (PD), supporting the \"body-first\" hypothesis. However, there remains a notable absence of PD-specific animal models induced by inflammatory cytokines. This study introduces a novel mouse model of PD driven by the proinflammatory cytokine CXCL1, identified in our previous research. The involvement of CXCL1 in PD pathogenesis was validated using subacute and chronic MPTP-induced mouse models. Based on these findings, 2-month-old C57BL/6J mice were intravenously administered CXCL1 (20 ng/kg/day) for 2 weeks (5 days per week), successfully replicating motor deficits and pathological alterations in the substantia nigra observed in the chronic MPTP model. These results demonstrate the potential of CXCL1-induced inflammation as a mechanism for PD modeling. The model revealed activation of the PPAR signaling pathway in CXCL1-mediated neuronal damage by CXCL1. Linoleic acid, a PPAR-γ activator, significantly mitigated MPTP- and CXCL1-induced toxicity and reduced serum CXCL1 levels. In addition, the CXCL1-injected mouse model shortened the timeline for developing chronic PD mouse model to 2 weeks, offering an efficient platform for studying inflammation-driven processes in PD. The findings provide critical insights into the inflammatory mechanisms underlying PD and identify promising therapeutic targets for intervention.</p>","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"46 1","pages":"225-235"},"PeriodicalIF":4.0,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11890994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ribosome profiling and single-cell RNA sequencing identify the unfolded protein response as a key regulator of pigeon lactation.","authors":"Jing Liu, San-Feng Liu, Hui-Rong Mao, Hong-Xia Jiang, Shui-Bing Liu, Xiao-Fei Xu, Jin-Tao Wu, Xun Liu, Wen-Tao Zhang, Xiao-Long Hu, Biao Chen","doi":"10.24272/j.issn.2095-8137.2024.336","DOIUrl":"10.24272/j.issn.2095-8137.2024.336","url":null,"abstract":"<p><p>Pigeons and certain other avian species produce a milk-like secretion in their crop sacs to nourish offspring, yet the detailed processes involved are not fully elucidated. This study investigated the crop sacs of 225-day-old unpaired non-lactating male pigeons (MN) and males initiating lactation on the first day after incubation (ML). Using RNA sequencing, ribosome profiling, and single-cell transcriptome sequencing (scRNA-seq), we identified a significant up-regulation of genes associated with ribosome assembly and protein synthesis in ML compared to MN. Results from scRNA-seq analysis identified 12 distinct cell types and 22 clusters, with secretory epithelial cells (SECs) exhibiting marked expression of plasma cell markers, including <i>IGLL1</i> and <i>MZB1</i>. RNA fluorescence <i>in situ</i> hybridization (RNA FISH) and IgY quantification confirmed the critical role of SECs in producing endogenous IgY during lactation. We propose that fibroblast-derived BAFF signals activate SECs, mimicking B cell transformation and enhancing protein production through the unfolded protein response (UPR). These findings shed light on the cellular dynamics of pigeon milk production and contribute to a broader understanding of avian biology.</p>","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"46 1","pages":"54-74"},"PeriodicalIF":4.0,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11890991/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the toxic effects of polystyrene nanoparticles on erythropoiesis at single-cell resolution.","authors":"Eun Jung Kwon, Hyeon Mi Sung, Hansong Lee, Soyul Ahn, Yejin Kim, Chae Rin Lee, Kihun Kim, Kyungjae Myung, Won Kyu Kim, Dokyoung Kim, Sanghwa Jeong, Chang-Kyu Oh, Yun Hak Kim","doi":"10.24272/j.issn.2095-8137.2024.277","DOIUrl":"10.24272/j.issn.2095-8137.2024.277","url":null,"abstract":"<p><p>Polystyrene nanoparticles pose significant toxicological risks to aquatic ecosystems, yet their impact on zebrafish ( <i>Danio rerio</i>) embryonic development, particularly erythropoiesis, remains underexplored. This study used single-cell RNA sequencing to comprehensively evaluate the effects of polystyrene nanoparticle exposure on erythropoiesis in zebrafish embryos. <i>In vivo</i> validation experiments corroborated the transcriptomic findings, revealing that polystyrene nanoparticle exposure disrupted erythrocyte differentiation, as evidenced by the decrease in mature erythrocytes and concomitant increase in immature erythrocytes. Additionally, impaired heme synthesis further contributed to the diminished erythrocyte population. These findings underscore the toxic effects of polystyrene nanoparticles on hematopoietic processes, highlighting their potential to compromise organismal health in aquatic environments.</p>","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"46 1","pages":"165-176"},"PeriodicalIF":4.7,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11890992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cross-species single-cell transcriptomics reveals neuronal similarities and heterogeneity in amniote pallium.","authors":"Fu-Bao-Qian Huang, Kuo Liao, Yu-Nong Sun, Zi-Hao Li, Yan-Ru Zhang, Ping-Fang Liao, Si-Yuan Jiang, Zhi-Yong Zhu, Duo-Yuan Chen, Ying Lei, Shi-Ping Liu, You-Ning Lin, Zhen-Kun Zhuang","doi":"10.24272/j.issn.2095-8137.2024.102","DOIUrl":"10.24272/j.issn.2095-8137.2024.102","url":null,"abstract":"<p><p>The amniote pallium, a vital component of the forebrain, exhibits considerable evolutionary divergence across species and mediates diverse functions, including sensory processing, memory formation, and learning. However, the relationships among pallial subregions in different species remain poorly characterized, particularly regarding the identification of homologous neurons and their transcriptional signatures. In this study, we utilized single-nucleus RNA sequencing to examine over 130 000 nuclei from the macaque ( <i>Macaca fascicularis</i>) neocortex, complemented by datasets from humans ( <i>Homo sapiens</i>), mice ( <i>Mus musculus</i>), zebra finches ( <i>Taeniopygia guttata</i>), turtles ( <i>Chrysemys picta bellii</i>), and lizards ( <i>Pogona vitticep</i>s), enabling comprehensive cross-species comparison. Results revealed transcriptomic conservation and species-specific distinctions within the amniote pallium. Notable similarities were observed among cell subtypes, particularly within <i>PVALB</i> ⁺ inhibitory neurons, which exhibited species-preferred subtypes. Furthermore, correlations between pallial subregions and several transcription factor candidates were identified, including <i>RARB</i>, <i>DLX2</i>, <i>STAT6</i>, <i>NR3C1</i>, and <i>THRB</i>, with potential regulatory roles in gene expression in mammalian pallial neurons compared to their avian and reptilian counterparts. These results highlight the conserved nature of inhibitory neurons, remarkable regional divergence of excitatory neurons, and species-specific gene expression and regulation in amniote pallial neurons. Collectively, these findings provide valuable insights into the evolutionary dynamics of the amniote pallium.</p>","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"46 1","pages":"193-208"},"PeriodicalIF":4.0,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11891007/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional evolution of thyrotropin-releasing hormone neuropeptides: Insights from an echinoderm.","authors":"Yingqiu Zheng, Huachen Liu, Xin Dang, Juan Diego Gaitán-Espitia, Muyan Chen","doi":"10.24272/j.issn.2095-8137.2024.256","DOIUrl":"10.24272/j.issn.2095-8137.2024.256","url":null,"abstract":"<p><p>Feeding behavior is regulated by a complex network of endogenous neuropeptides. In chordates, this role is suggested to be under the control of diverse factors including thyrotropin-releasing hormone (TRH). However, whether this regulatory activity of TRH is functionally conserved in non-chordate metazoans, and to what extent this process is underpinned by interactions of TRH with other neuropeptides such as cholecystokinin (CCK, known as a satiety signal), remain unclear. This study investigated the TRH signaling system in the echinoderm <i>Apostichopus</i> <i>japonicus</i>. Bioinformatic analyses and ligand-binding assays identified a functional TRH receptor (AjTRHR) that activated signaling via the MAPK/ERK1/2 pathways. Experimental administration of TRH significantly reduced feeding activity, while up-regulating CCK expression. RNA interference (RNAi) experiments confirmed that both CCK and TRH are essential components of satiety signaling, working synergistically to mediate feeding inhibition. Evolutionary analysis of TRH-type peptides revealed greater conservation of the short isoform of TRH compared to the long isoform, probably driven by strong selection acting on the functional redundancy. These findings provide compelling evidence of a TRH-mediated signaling system in non-chordate deuterostomes, expanding our understanding of neuropeptide-regulated feeding mechanisms in marine invertebrates.</p>","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"46 1","pages":"236-248"},"PeriodicalIF":4.0,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11890989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA2 knockout aggravates cerebral ischemia/reperfusion injury by reducing postsynaptic Homer1a.","authors":"Ting Ma, Yu-Meng Li, Peng-Yu Ren, Shi-Quan Wang, Xiang-Long Liu, Wen-Bo Lv, Wu-Gang Hou, Wen-Qiang Zuo, Wei-Qiang Lin, Jian Sima, An-Qi Geng","doi":"10.24272/j.issn.2095-8137.2024.269","DOIUrl":"10.24272/j.issn.2095-8137.2024.269","url":null,"abstract":"<p><p>DNA2, a multifunctional enzyme with structure-specific nuclease, 5 <i>'</i>-to-3 <i>'</i> helicase, and DNA-dependent ATPase activities, plays a pivotal role in the cellular response to DNA damage. However, its involvement in cerebral ischemia/reperfusion (I/R) injury remains to be elucidated. This study investigated the involvement of DNA2 in cerebral I/R injury using conditional knockout (cKO) mice ( <i>Nestin</i>-Cre) subjected to middle cerebral artery occlusion (MCAO), an established model of cerebral I/R. Results demonstrated a gradual up-regulation of DNA2 expression, peaking at 72 h post-MCAO. Notably, <i>DNA2</i> cKO mice exhibited more pronounced brain injury, neurological deficits, and neuronal apoptosis within the penumbra following MCAO. Additionally, DNA2 expression was elevated in an oxygen-glucose deprivation/reoxygenation (OGD/R) cell culture model, and <i>DNA2</i> knockdown (KD) exacerbated neuronal apoptosis and oxidative stress. Transcriptome analysis of ischemic penumbra tissues via RNA sequencing revealed significant down-regulation of <i>Homer1</i> in <i>DNA2</i> cKO mice. Furthermore, <i>in</i> <i>vitro</i> experiments demonstrated that overexpression of <i>Homer1a</i> ameliorated <i>DNA2</i> KD-induced neuronal apoptosis. Collectively, these findings demonstrate that <i>DNA2</i> deficiency exacerbates cerebral I/R injury through the down-regulation of <i>Homer1a</i>, highlighting a novel regulatory axis in ischemic neuroprotection.</p>","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"46 1","pages":"87-102"},"PeriodicalIF":4.0,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11890998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Both 20S and 19S proteasome components are essential for meiosis in male mice.","authors":"Ting-Ting Han, Li-Ying Wang, Qiu-Xing Zhou, Wei Wei, Yan-Jie Ma, Ying-Hong Chen, Wei Li, Zhen-Yu Ju, Chao Liu","doi":"10.24272/j.issn.2095-8137.2024.281","DOIUrl":"10.24272/j.issn.2095-8137.2024.281","url":null,"abstract":"<p><p>The proteasome, an evolutionarily conserved proteolytic complex comprising the 20S core particle and 19S regulatory particles, performs both shared and distinct functions across various tissues and organs. Spermatogenesis, a highly complex developmental process, relies on proteasome activity at multiple stages to regulate protein turnover. In this study, we selected the 20S subunit PSMA1 and 19S regulatory subunit PSMD2 to investigate the potential functions of the proteasome in spermatogenesis. Using <i>Psma1-EGFP</i> and <i>Psmd2-mCherry</i> knock-in mouse models, we confirmed the expression of both subunits in all spermatogenic cell types, with pronounced presence in early germ cell development. To further clarify their functional significance, we specifically knocked out <i>Psma1</i> and <i>Psmd2</i> in germ cells. Deletion of either PSMA1 or PSMD2 led to disrupted spermatogenesis, characterized by the complete absence of sperm in the epididymis. Subsequent analysis indicated that loss of these proteasome components impaired meiotic initiation. <i>Psma1</i> and <i>Psmd2</i> knockout germ cells showed accumulation of DMRT1, a key regulator of mitosis-to-meiosis transition, leading to a reduction in STRA8 levels and consequent disruption of meiosis initiation. This study sheds light on the molecular mechanisms that govern meiotic initiation and identifies potential genes associated with male infertility.</p>","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"46 1","pages":"27-40"},"PeriodicalIF":4.0,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11891003/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intranasal iron administration induces iron deposition, immunoactivation, and cell-specific vulnerability in the olfactory bulb of C57BL/6 mice.","authors":"Xiao-Qing Mi, Bao-Chen Liu, Le Qu, Yu Yuan, Hui Li, Ao-Yang Xu, Yu-Lin Zhang, Jun-Xia Xie, Ning Song","doi":"10.24272/j.issn.2095-8137.2024.240","DOIUrl":"10.24272/j.issn.2095-8137.2024.240","url":null,"abstract":"<p><p>Iron is the most abundant transition metal in the brain and is essential for brain development and neuronal function; however, its abnormal accumulation is also implicated in various neurological disorders. The olfactory bulb (OB), an early target in neurodegenerative diseases, acts as a gateway for environmental toxins and contains diverse neuronal populations with distinct roles. This study explored the cell-specific vulnerability to iron in the OB using a mouse model of intranasal administration of ferric ammonium citrate (FAC). Olfactory function was assessed through olfactory discrimination tests, while iron levels in OB tissues, cerebrospinal fluid (CSF), and serum were quantified using inductively coupled plasma mass spectrometry (ICP-MS), immunohistochemical staining, and iron assays. Transcriptomic changes and immune responses were assessed using RNA sequencing and immune cell infiltration analysis. Results showed that intranasal FAC administration impaired olfactory function, accompanied by iron deposition in the olfactory mucosa and OB, as well as damage to olfactory sensory neurons. Notably, these effects occurred without elevations in CSF or serum iron levels. OB iron accumulation activated multiple immune cells, including microglia and astrocytes, but did not trigger ferroptosis. Spatial transcriptomic sequencing of healthy adult mouse OBs revealed significant cellular heterogeneity, with an abundance of neuroglia and neurons. Among neurons, GABAergic neurons were the most prevalent, followed by glutamatergic and dopaminergic neurons, while cholinergic and serotonergic neurons were sparsely distributed. Under iron-stressed conditions, oligodendrocytes, dopaminergic neurons, and glutamatergic neurons exhibited significant damage, while GABAergic neurons remained unaffected. These findings highlight the selective vulnerability of neuronal and glial populations to iron-induced stress, offering novel insights into the loss of specific cell types in the OB during iron dysregulation.</p>","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"46 1","pages":"209-224"},"PeriodicalIF":4.0,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11891001/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The golden era of scientific publishing in China.","authors":"Yong-Gang Yao, Yong-Tang Zheng","doi":"10.24272/j.issn.2095-8137.2024.465","DOIUrl":"10.24272/j.issn.2095-8137.2024.465","url":null,"abstract":"","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"46 1","pages":"1-2"},"PeriodicalIF":4.0,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11891002/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced risk assessment framework integrating distribution dynamics, genetically inferred populations, and morphological traits of <i>Diploderma</i> lizards.","authors":"Qi Xiao, Xiu-Dong Shi, Lin Shi, Zhong-Yi Yao, You-Hua Chen, Wei-Zhao Yang, Zi-Yan Liao, Yin Qi","doi":"10.24272/j.issn.2095-8137.2024.287","DOIUrl":"10.24272/j.issn.2095-8137.2024.287","url":null,"abstract":"<p><p>Assessing the threat status of species in response to global change is critical for biodiversity monitoring and conservation efforts. However, current frameworks, even the IUCN Red List, often neglect critical factors such as genetic diversity and the impacts of climate and land-use changes, hindering effective conservation planning. To address these limitations, we developed an enhanced extinction risk assessment framework using <i>Diploderma</i> lizards as a model. This framework incorporates long-term field surveys, environmental data, and land-use information to predict distributional changes for 10 recently described <i>Diploderma</i> species on the Qinghai-Xizang Plateau, which hold ecological significance but remain underassessed in conservation assessment. By integrating the distribution data and genetically inferred effective population sizes ( <i>N</i>e), we conducted scenario analyses and used a rank-sum approach to calculate Risk ranking scores (RRS) for each species. This approach revealed significant discrepancies with the IUCN Red List assessments. Notably, <i>D. yangi</i> and <i>D. qilin</i> were identified as facing the highest extinction risk. Furthermore, <i>D. vela</i>, <i>D. batangense</i>, <i>D. flaviceps</i>, <i>D. dymondi</i>, <i>D. yulongense</i>, and <i>D. laeviventre</i>, currently classified as \"Least Concern\", were found to warrant reclassification as \"Vulnerable\" due to considerable threat from projected range contractions. Exploring the relationship between morphology and RRS revealed that traits such as snout-vent length and relative tail length could serve as potential predictors of extinction risk, offering preliminary metrics for assessing species vulnerability when comprehensive data are unavailable. This study enhances the precision of extinction risk assessment frameworks and demonstrates their capacity to refine and update risk assessments, especially for lesser-known taxa.</p>","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"46 1","pages":"15-26"},"PeriodicalIF":4.0,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11890995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}