Rhizosphere最新文献

{"title":"Nutrient and stoichiometric characteristics of various organs in three typical desert plants from extreme desert ecosystems","authors":"Yi Du ,&nbsp;Yulin Zhang ,&nbsp;Zhihao Zhang ,&nbsp;Yanju Gao ,&nbsp;Zhaobin Mu ,&nbsp;Waqar Islam ,&nbsp;Fanjiang Zeng","doi":"10.1016/j.rhisph.2025.101025","DOIUrl":"10.1016/j.rhisph.2025.101025","url":null,"abstract":"<div><div>Predicting how fragile and sensitive desert ecosystems will react to environmental changes depends on understanding how soil factors influence plant nutrient concentration and stoichiometry. We studied nutrients and stoichiometric characteristics of various organs of three desert plants (<em>Alhagi sparsifolia</em>, <em>Tamarix ramosissima</em>, and <em>Calligonum caput-medusae</em>) in Turpan (TLF), Tarim (CL), and Dzungaria (MSW) basins of Xinjiang, China. The results showed that in three regions (CL, MSW, and TLF), the nutrient levels of total nitrogen (TN) and total potassium (TK) in the leaves, roots, and branches of <em>A. sparsifolia</em> were significantly higher than those of <em>C. caput-medusae</em>. The total phosphorus (TP) contents in the leaves, roots, and branches of <em>A. sparsifolia</em> were significantly higher than those of <em>T. ramosissima</em> and <em>C. caput-medusae</em>. However, the nitrogen-to-phosphorus ratio in the leaf of <em>C. caput-medusae</em> was significantly lower compared with <em>A. sparsifolia</em> and <em>T. ramosissima</em>. <em>A. sparsifolia</em> had higher root organic carbon content compared with its branches and leaves, while its leaves had higher nutrient levels of TN and TK compared with its roots and branches. The leaf organic carbon contents of <em>T. ramosissima</em> and <em>C. caput-medusae</em> were lower compared with roots and branches, but higher leaf nutrients (TN, TP, and TK contents) than in roots and branches. The soil nutrients of three desert plants experienced significant nitrogen and phosphorus element limitations. Soil electrical conductivity (EC) was identified as a common environmental factor influencing the nutrient changes in the leaves, branches, and roots of desert plants. The structural equation model that soil pH was positively correlated with branch and root stoichiometry, however, soil EC was negatively correlated with root stoichiometry. This research offers a scientific foundation and essential information for the conservation and rehabilitation of desert ecosystems.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101025"},"PeriodicalIF":3.4,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143379141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of rhizosphere soil microenvironment interaction on ginsenoside content in Panax ginseng: A case study of three-year-old agricultural ginseng","authors":"Zhefeng Xu ,&nbsp;Yuqiu Chen ,&nbsp;Rui Liu ,&nbsp;Yibing Wang ,&nbsp;Chunshuo Liu ,&nbsp;Jing Fang ,&nbsp;Qinghe Zhang ,&nbsp;Tao Zhang ,&nbsp;Changbao Chen","doi":"10.1016/j.rhisph.2025.101023","DOIUrl":"10.1016/j.rhisph.2025.101023","url":null,"abstract":"<div><div>Planting ginseng (<em>Panax ginseng</em> C. A. Meyer) in farmland is the main method of ginseng cultivation, but the interaction between the rhizosphere soil microenvironment and the mechanism of ginsenoside accumulation in ginseng roots are still unclear. Therefore, the content of ginsenoside and rhizosphere soil microenvironment of 3-year-old agricultural ginseng at two growth periods (May and October) were studied. The results showed that the content of bulk density, pH, alkaline nitrogen and organic matter in rhizosphere soil microenvironment significantly decreased. There were significant changes in the activities of carbon cycle related enzymes (amylase, invertase, cellulase etc.), nitrogen cycle related enzymes (urease, uricase, nitrate reductase, etc.), phosphorus cycle related enzymes (acid phosphatase, phytase), and sulfur cycle related enzymes (arylsulfatase) in rhizosphere. The decrease in keystone microbial diversity in a co-occurrence network was a manifestation of soil degradation at the biological level. Ginsenosides showed significant accumulation, with a 42.4% increase in the total content of ginsenosides. Environmental factors (pH, cellulase, and cation exchange capacity) significantly affected the accumulation of ginsenosides. The results of the co-occurrence network indicated that fungal communities were more susceptible to environmental factors than bacterial communities. Meanwhile, the structure and diversity of the fungal communities had a more significant impact on the accumulation of ginsenosides compared to the bacterial community. Comprehensive analysis showed that the interaction between environmental factors (pH, cellulase, cation exchange capacity) and soil microbiome (<em>Coprinellus</em>, <em>Agaricales_unclassified</em>, <em>Mortierella</em>) may be the key factor affecting ginsenoside accumulation in 3-year-old agricultural ginseng. The research results provide reference for soil environment improvement and the development of appropriate management measures based on the fertilizer requirements of ginseng, which can help achieve sustainable production of agricultural ginseng.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101023"},"PeriodicalIF":3.4,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143386535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential stimulation of phosphorus-mobilizing bacteria by common bean genotypes in Amazonian Dark Earth and Agricultural Soils with varying fertility levels","authors":"Mariley Fonseca ,&nbsp;Alexandre Pedrinho ,&nbsp;Luis Fernando Merloti ,&nbsp;João William Bossolani ,&nbsp;Leandro Nascimento Lemos ,&nbsp;Mayara Martins e Martins ,&nbsp;Lucas William Mendes ,&nbsp;Tsai Siu Mui","doi":"10.1016/j.rhisph.2025.101026","DOIUrl":"10.1016/j.rhisph.2025.101026","url":null,"abstract":"<div><div>Phosphorus (P) is an essential element for plant metabolism and often limits agricultural production due to its strong immobilization in soils. Microbial activity plays a crucial role in nutrient cycling, particularly in mobilizing P for plant uptake. This study evaluated how plants with different P use efficiencies influence the structure, diversity, and composition of the microbial community, with a focus on P-mobilizing bacteria, in soils with varying fertility levels. We hypothesized that plants with contrasting P use efficiencies differentially recruit rhizosphere microbiota associated with the P cycle. To test this hypothesis, a greenhouse experiment was conducted using two common bean (<em>Phaseolus vulgaris</em> L.) genotypes, BAT 477 (G1) and DOR 364 (G2), known for their contrasting P assimilation efficiencies. The common beans were grown in Amazonian Dark Earth (ADE) and agricultural soil (AS) until the pre-flowering phenological stage (R₅). Rhizosphere and bulk soil (BS) samples were collected for microbiological, enzymatic, and P fractionation analyses. Amplicon sequencing revealed that the composition and relative abundance of the prokaryotic community were significantly influenced by the common bean genotypes across the different soil types. Acid phosphatase activity was approximately 22% higher in the rhizosphere than in BS for both genotypes, irrespective of soil type. Alkaline phosphatase activity, however, exhibited distinct patterns: in ADE, G1 showed 48% higher activity than BS, while in AS, G2 displayed the highest activity, with a 33.6% increase compared to BS. Redundancy analysis (RDA) in ADE revealed three distinct clusters (G1 ≠ G2 ≠ BS), indicating genotype-specific microbial recruitment patterns with significant positive correlations to the moderately labile P fraction. In AS, only two clusters were observed (G1+G2 and BS), with positive correlations to the moderately labile P fraction but no distinction between genotypes. These findings demonstrate that plants with differing P assimilation efficiencies distinctly modulate the rhizosphere microbiome, influencing P mobilization in a soil fertility-dependent manner. This study highlights the potential of leveraging plant-microbe interactions to enhance P cycling and utilization in agricultural systems, paving the way for more sustainable and efficient crop production practices.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101026"},"PeriodicalIF":3.4,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143403734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of environmental factors and genotype on performance, soil physicochemical properties and endophytic fungi of Salvia miltiorrhiza","authors":"Xin Zheng ,&nbsp;Wenjing Chen ,&nbsp;Xianen Li ,&nbsp;Wenyi Shi ,&nbsp;Xiang Sun ,&nbsp;Qieyun Ge ,&nbsp;Chao He ,&nbsp;Xueli He","doi":"10.1016/j.rhisph.2025.101031","DOIUrl":"10.1016/j.rhisph.2025.101031","url":null,"abstract":"<div><div><em>Salvia miltiorrhiza</em> Bge. is a widely planted perennial medicinal herb, and screening excellent genotype resources and suitable cultivation areas is an effective way to enhance its quality and quantity. Here, the effects of environmental conditions and genotypes on plant performance, root endophytic fungi, and soil physicochemical properties were evaluated by collecting plant and rhizosphere soil samples from four regions (Beijing, Henan, Shandong, and Sichuan) and four genotypes (Z1, Z2, Z3, and Z4). The results indicated that different genotypes of <em>S. miltiorrhiza</em> from different regions showed heterogeneity in various indicators. Each genotype showed specific growth performances to geographic locations, and the peak of lipid- and water-soluble medicinal ingredient contents occurred in the summer or winter due to different genotypes. In addition, the distribution of root endophytic fungi in each genotype depended on season and site environment. Variation partitioning analysis showed that medicinal ingredients and endophytic fungal isolation rates were primarily influenced by climatic factors, whereas plant growth indicators were mainly influenced by the interaction of climatic factors, growth period, and soil factors. Correlation analysis and SEM indicated that soil pH, available nitrogen, and available phosphorus were the main factors affecting plant performance and medicinal ingredients. Based on growth indicators, Z3 was suitable for planting in Shandong, whereas Z4 was suitable for planting in Sichuan. Based on medicinal ingredients, all genotypes were suitable for cultivation in Beijing. These results not only help to comprehend the ecological adaptability of different genotypes of <em>S. miltiorrhiza</em> but also have guiding significance for regional cultivation.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101031"},"PeriodicalIF":3.4,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143379408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stimulating effect of increasing N fertilization on plant growth and rhizosphere microbial activity at low levels of N2O emissions","authors":"Jaroslav Hynšt ,&nbsp;Ivan Tůma ,&nbsp;Irina Mikajlo ,&nbsp;Jaroslav Záhora","doi":"10.1016/j.rhisph.2025.101032","DOIUrl":"10.1016/j.rhisph.2025.101032","url":null,"abstract":"<div><div>A greenhouse experiment was conducted to assess the short-term effects of nitrogen (N) addition on plant growth, rhizosphere microbial activity, and N₂O emissions. Nitrogen was applied to lettuce plants once the roots had occupied the entire volume of the pots, ensuring that the whole soil volume could be considered rhizosphere soil. The hypothesis tested was that adding a low amount of plant-available nitrogen to the rhizosphere would increase plant root growth and root-associated microbial activity with minimal N₂O emissions. Conversely, when the nitrogen availability exceeds the demands of the plant-soil system, it decreases root and microbial growth while increasing N₂O emissions. Results indicated that nitrogen applied at rates of 0–100 mg N kg⁻¹ was efficiently utilized by plants, supporting the growth of both aboveground and belowground plant biomass and microbial biomass. Notably, the addition of nitrogen did not increase N₂O production. These findings suggest that dense root systems can efficiently utilize relatively high amounts of nitrogen.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101032"},"PeriodicalIF":3.4,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143403736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant-microbial interplay for organic nitrogen mediated by functional specificity of root compartments","authors":"Guoting Shen ,&nbsp;Andrey Guber ,&nbsp;Sajedeh Khosrozadeh ,&nbsp;Negar Ghaderi ,&nbsp;Alexandra Kravchenko ,&nbsp;Evgenia Blagodatskaya","doi":"10.1016/j.rhisph.2025.101024","DOIUrl":"10.1016/j.rhisph.2025.101024","url":null,"abstract":"<div><div>The organic form of nitrogen (N) is a critical intermediate in mutualistic and competitive root-microbial interactions, mediated by extracellular enzymes. Visualization of the hotspots of organic N and proteolytic activity might be valuable for revealing root functional specificity in N acquisition and transformation at the level of individual roots and compartments. For the first time, we used time-lapse amino-mapping and zymography to co-localize and map the spatial distribution of amino-N and leucine aminopeptidase (LAP) activity in the soil and different root parts of maize (<em>Zea mays</em> L.). Amino-N distribution was mainly associated with seminal roots and root tips, where it overlapped with LAP activity hotspots. In the lateral roots and bulk soil, however, LAP activity was decoupled from amino-N. Distinct functional traits revealed themselves as the highest amino-N content and LAP activity in seminal root tips and as the largest relative extent of the rhizosphere in lateral root tips. Co-localized amino-N and LAP activities highlighted different nutrient acquisition strategies mediated by root-microbe interactions, depending on the root compartment. Seminal roots and their tips appeared to adopt mutualistic strategies, potentially attracting root-associated microorganisms through releasing oligo- and polypeptides. In contrast, lateral roots, with amino-N detected only at their tips, demonstrated stronger N competition, relying on the enzyme activity of the rhizosphere microbial community for N acquisition. These insights emphasized the role of root functional specialization in shaping plant-microbe interactions, offering pathways to enhance nutrient use efficiency.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101024"},"PeriodicalIF":3.4,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multilevel analysis of Azospirillum biofilms and cystlike forms and characterization of their possible role in wheat drought tolerance","authors":"Andrei Shelud'ko,&nbsp;Irina Volokhina,&nbsp;Dmitry Mokeev,&nbsp;Elizaveta Telesheva,&nbsp;Yulia Filip'echeva,&nbsp;Andrei Burov,&nbsp;Igor Borisov,&nbsp;Alexander Shirokov,&nbsp;Larisa Matora,&nbsp;Lilia Petrova","doi":"10.1016/j.rhisph.2025.101029","DOIUrl":"10.1016/j.rhisph.2025.101029","url":null,"abstract":"<div><div>The type strains <em>Azospirillum brasilense</em> Sp7 and <em>A. baldaniorum</em> Sp245, capable of penetrating plant roots, formed mono- and multilayered biofilms on the wheat root surface both in sterile aqueous medium and in nonsterile soil. The biofilms formed on an abiotic surface under hydroponic conditions also were mono- and multilayered. In the wheat root system, the azospirilla produced biofilms mainly on the root apex, the root hairs, and the areas of lateral root development, regardless of the growth conditions. It was exactly in these root zones that a lectin identical to wheat germ agglutinin had previously been detected, and a hapten specific for this lectin was present in the biofilm matrix of the strains under study. The synthesis of osmoprotectants and glycopolymers and the formation of cystlike forms by <em>Azospirillum</em> contributed to biofilm survival during drying. The biofilm components played a part in increasing the resistance of the <em>Azospirillum</em>-inoculated plants to drought-simulating conditions in soil. The same components played a role in increasing the resistance of <em>Azospirillum</em>-inoculated plants to simulated soil drought. Drought was simulated by stopping soil watering for 10 days. The Sp7-derived phenotypic variant Sp7.8, which develops large cystlike forms with a complex morphology, not only remained viable during biofilm desiccation but also protected the plants from the negative effects of soil drought more successfully than did the parent strain. In biofilms, the phenotypic variant Sp7.8 formed 1.8 times more cystlike forms (under N-sufficient conditions) than did strain Sp7.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101029"},"PeriodicalIF":3.4,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143403735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphorus bioavailability and silicon fractionation in wheat rhizosphere affected by soil water content and silicon application","authors":"Meysam Cheraghi ,&nbsp;Babak Motesharezadeh ,&nbsp;Seyed Majid Mousavi ,&nbsp;Majid Basirat ,&nbsp;Hossein Ali Alikhani","doi":"10.1016/j.rhisph.2025.101017","DOIUrl":"10.1016/j.rhisph.2025.101017","url":null,"abstract":"<div><div>Drought stress limits plant growth by reducing water and nutrient uptake. This study examined the effects of silicon (Si) and soil water content (SWC) on Si fractionation and phosphorus (P) bioavailability in the wheat rhizosphere, as well as their uptake under drought stress. The experimental setup involved dividing pot soil into rhizospheric and non-rhizospheric (bulk) zones using rhizobags. Wheat seeds were grown under different Si treatments (0, 150, 300 mg/kg monosilicic acid, and 150, 300 mg/kg Si nanoparticles) and SWC (0.4 and 0.8 Field Capacity). Results demonstrated that plant-available Si and adsorbed Si concentrations in the rhizosphere were 16.3% and 10.8% higher than in bulk soil, respectively, while amorphous Si was 17.4% higher in bulk soil. Drought stress decreased plant-available Si and adsorbed Si in the rhizosphere by 14.9% and 10.8%, respectively, while increased amorphous Si by 8.2%. Si application enhanced plant-available Si, adsorbed Si, and amorphous Si in both rhizosphere and bulk soil. Phosphorus bioavailability in the rhizosphere was 1.44 times higher than in bulk soil, and drought stress reduced plant-available P by 9.1% and 6% in the rhizosphere and bulk, respectively. Si treatments increased P bioavailability in the rhizosphere by 10.3–21.2%. Reduced rhizosphere pH (up to 0.5–1 unit) and increased organic carbon (up to 19–48%) mediated by Si contributed to higher P availability. Drought stress decreased leaf P content by 15–48%, while Si treatments increased it by 55.4–148.5%. Si-mediated improvements in P uptake were linked to enhanced water uptake, as indicated by a 23.1–37.3% increase in leaf water content and an 11.4–36.4% increase in transpiration rate under drought stress. These findings highlight Si's role in improving wheat drought tolerance by modifying rhizosphere properties and facilitating water and nutrient uptake. Therefore Si application can be considered in fertilization programs in arid and semi-arid regions.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101017"},"PeriodicalIF":3.4,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The root extracellular trap: A checkpoint controlling root tip accessibility to microorganisms","authors":"Alexia Gaudry ,&nbsp;Magalie Bénard ,&nbsp;Agnès Attard ,&nbsp;Eric Nguema-Ona ,&nbsp;Azeddine Driouich ,&nbsp;Barbara Pawlak","doi":"10.1016/j.rhisph.2025.101016","DOIUrl":"10.1016/j.rhisph.2025.101016","url":null,"abstract":"<div><div>The root tip and root-Associated Cap-Derived Cells (AC-DCs) release various organic compounds into the rhizosphere, forming the Root Extracellular Trap (RET), a network involved in root-soil microorganism interactions and root protection. This study investigates the role of soybean (<em>Glycine max</em>) and pea (<em>Pisum sativum</em>) RETs in interactions with soil rhizobacteria (<em>Bacillus subtilis</em> and <em>Pseudomonas fluorescens</em>) and the zoospores of the oomycete <em>Phytophthora parasitica</em> by examining their impact on microbial behavior. To this end, confrontation tests were performed, followed by imaging analyses of videos, in which the behavior of microorganisms (<em>i.e.,</em> swimming speeds and trajectories) was characterized and quantified. The results show that the RET alters microbial access to the root tip and modifies significantly speeds and trajectories. Inside the RET, the speeds of <em>B. subtilis</em> and <em>P. parasitica</em> zoospores decreased three and nine times respectively. Outside the RET, the speed of the bacterium remains unchanged, while that of the zoospores decreases twice. These findings highlight the influence of the RET on microbial movement and its importance in plant-microorganism interactions in the rhizosphere.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101016"},"PeriodicalIF":3.4,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diverging role of phytohormones and soil nutrients between two broad and narrow-distribution orchids of Satyrium species","authors":"Brihaspati Poudel,&nbsp;Taiqiang Li,&nbsp;Rengasamy Anbazhakan,&nbsp;Jiangyun Gao","doi":"10.1016/j.rhisph.2024.101013","DOIUrl":"10.1016/j.rhisph.2024.101013","url":null,"abstract":"<div><div>The community assembly of root-associated endophytic fungi not depend on the distribution pattern of host orchids at the local level. In each case, the host plant uses soil nutrients and host compounds to attract endophytic fungi; yet, it is still necessary to investigate how these factors affect the distribution of sample orchids and their associates. Therefore, we assessed the diverging role of phytohormones and soil nutrients in their host distribution. The root-associated fungi were investigated using the amplicon sequencing method and evaluated the host chemical profiles/soil nutrients associated with the roots of sample species using Gas Chromatography-Mass Spectrometry and High Performance-Liquid Chromatography. Finally, we used the correlation analysis method to examine their relationship with endophytic fungi. Using soil samples that were taken, we assessed nine soil nutrients along with soil pH. Likewise, 22 phytohormones were found in the roots of sample orchids. Where, both sample orchids (<em>Satyrium nepalense</em> D. Don. and <em>Satyrium yunnanense</em> Rolfe.) were dominated by distinct endophytic fungal families (Unclassified_k_fungi and Hygrophoraceae) independently, and they showed either positive or negative correlation with phytohormones and soil nutrients based on the distribution pattern of host orchids. The narrowly-distributed orchid was rich by soil nutrients and endophytic fungi but the broadly-distributed orchid was rich by phytohormones and poor in fungi composition in local level So, the diversity and community composition of endophytic fungi was not related to phytohormones but positively related with soil nutrients in local level. Our limited data cannot provide strong evidence but this finding may open the door for further study over the distribution of orchids and their symbionts. Finally, our results help in ecological resilience and potential biotechnological application or conservation of orchids and their symbionts.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101013"},"PeriodicalIF":3.4,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}