Rhizosphere最新文献

{"title":"Inoculation of soybean rhizosphere microorganisms could promote corn growth","authors":"Siqi Yu,&nbsp;Yi Shen,&nbsp;Yaohui Yang,&nbsp;Zhenyan Zhang,&nbsp;Jichao Zhu,&nbsp;Qingshan Xia,&nbsp;Minglong Song,&nbsp;Binghai Lv,&nbsp;Liwei Sun,&nbsp;Haifeng Qian,&nbsp;Tao Lu","doi":"10.1016/j.rhisph.2025.101047","DOIUrl":"10.1016/j.rhisph.2025.101047","url":null,"abstract":"<div><div>Rhizosphere microbes are essential for crop growth and development. Soybean rhizosphere microbes are believed to have nitrogen-fixing functions that promote plant growth. However, it is not known whether soybean rhizosphere microbes promote the growth of other crops via transplantation. In this study, we investigated the potential of soybean-derived rhizosphere microbes to promote corn growth, included a significant increase (5.3%) in corn plant height. The inoculation of soybean rhizosphere microbes did not significantly affect microbial diversity in the corn rhizosphere, but the relative abundance of plant-beneficial bacteria increased significantly by 23.12%, particularly the genera <em>Nocardioides</em>, <em>Variovorax</em>, <em>Pseudoalteromonas</em>, <em>Bosea</em> and <em>Adhaeribacter</em>. Furthermore, inoculation with soybean rhizosphere microbes promoted the expression of metabolic genes involved in carbohydrate metabolism, amino acid metabolism, and membrane transport, which in turn accelerated nutrient cycling in the soil and promoted plant growth. Our study shows that soybean can enrich many beneficial plant bacteria in rhizosphere whose function is beyond nitrogen fixation, and these enriched beneficial bacteria can be applied to other crops, such as corn, by transferring rhizosphere soil, providing new insights into rhizosphere microbial inoculation techniques and contribute to our understanding of the ecological functions associated with rhizosphere microbes.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101047"},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of integrated nutrient management on soil microbiome diversity and health in rice based cropping system: Insights from long-term agricultural practices","authors":"Subhojit Datta ,&nbsp;Sonali Paul Mazumdar ,&nbsp;Bijan Majumdar ,&nbsp;N.M. Alam ,&nbsp;Lipi Chattopadhyay ,&nbsp;Sourav Ghosh ,&nbsp;Dipnarayan Saha ,&nbsp;Amit Ranjan Saha ,&nbsp;Gouranga Kar","doi":"10.1016/j.rhisph.2025.101048","DOIUrl":"10.1016/j.rhisph.2025.101048","url":null,"abstract":"<div><div>The rhizosphere soil microbiomes, which are essential for plant development, stress adaptability, and general soil health, are greatly impacted by agricultural management practices, particularly those involving nutrient applications. This study evaluated the long-term effects of nutrient management practices on soil physicochemical properties, microbial communities, enzyme activities, and biological soil health in a rice-lentil-jute cropping system. The treatments included a control, inorganic fertilizers (recommended dose of fertilizers), and Soil Test Crop Response based integrated nutrient management (combination of inorganic fertilizer based on soil test-based fertilizer prescription equations, farmyard manure (FYM), and bioinoculants). When compared to both control and inorganic treatments, integrated nutrient management (INM) enhanced soil organic carbon, available nitrogen, phosphorus, and potassium. Microbial populations, comprising of bacteria, actinomycetes, fungi, <em>Azotobacter</em>, and phosphate-solubilizing microorganisms, along with soil enzymatic activities, showed marked increases under INM. Metagenomic analysis of the hypervariable V3-V4 region of 16S rRNA indicated that the bacterial community in the rice-lentil-jute cropping sequence was dominated by <em>Proteobacteria</em>, with 58 phyla having over 1% abundance. The INM treatment increased the Shannon diversity index by 12.6% compared to the control, reflecting improved microbial diversity, richness, and resilience, which are critical for enhancing crop productivity and stress tolerance. The Biological Soil Health Index (BSHI) was highest in the INM treatment, with average contributions from <em>Azotobacter</em> (22.7%), <em>Bacteroidota</em> (12.1%), <em>Actinobacteriota</em> (21.9%)<em>,</em> very labile organic carbon (23.1%)<em>,</em> and labile organic carbon (20.2%) to BSHI. In summary, ten years of INM enhanced soil health and bacterial community structure and composition, leading to sustainable crop yields in rice-based cropping system. These findings highlight the necessity of integrating balanced nutrient management in long-term agricultural practices.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101048"},"PeriodicalIF":3.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing the scale of spatial aggregation in minirhizotron studies of crop root system distribution","authors":"Simon Riley,&nbsp;Edzard van Santen","doi":"10.1016/j.rhisph.2025.101041","DOIUrl":"10.1016/j.rhisph.2025.101041","url":null,"abstract":"<div><div>Research into root system distribution often employs analyses in which depth is treated as a categorical variable. It is not presently known to what extent the choice of strata size affects type I and type II error rates in such analyses, or how to maximize statistical power while controlling for false positives. This research addresses those questions using a simulation study: mixed models were fit to each of one thousand simulated data sets, for 400 treatment combinations associated with differing levels of spatial and temporal autocorrelation, different effect sizes, and different degrees of spatial aggregation. The results show that statistical power declined with increasing degrees of aggregation, especially for small effect sizes and in the presence of spatial autocorrelation. Specifically, in the absence of spatial autocorrelation and with a true effect size of 6, aggregating 80 data points into 4, 20 cm depth class reduced statistical power from a very high initial rate of 0.946 (95% Confidence Interval: 0.935–0.955) to the still acceptable rate of 0.855 (0.839–0.870), but for an effect size of just one, initial power was already lower, at 0.656 (0.635–0.677) when no aggregation was performed and fell to just 0.373 (0.352–0.395) upon aggregating to 20 cm depth classes. This pattern is even more pronounced in the presence of spatial autocorrelation. Overall, the study recommends that researchers choosing to employ such an analysis for their minirhizotron data use the smallest computationally feasible depth classes.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101041"},"PeriodicalIF":3.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrogen deficiency drives fungal compositional shifts without functional changes in wheat rhizosphere","authors":"Lok Hang Chan ,&nbsp;Shu Kee Lam ,&nbsp;Deli Chen ,&nbsp;Caixian Tang ,&nbsp;Qinglin Chen ,&nbsp;Ute Roessner ,&nbsp;Hang-Wei Hu","doi":"10.1016/j.rhisph.2025.101030","DOIUrl":"10.1016/j.rhisph.2025.101030","url":null,"abstract":"<div><div>Nitrogen (N) deficiency reduces crop yield, but this effect may be mitigated by symbiotic interactions between crops and fungi. However, the response of wheat-fungal interactions to N deficiency remains unclear. We hypothesised that wheat cultivars with a higher reported nitrogen use efficiency (NUE), would induce shifts in the fungal community composition and functional profiles within the wheat rhizosphere to tolerate N deficiency. A glasshouse experiment was conducted to examine the effects of N deficiency on the rhizosphere fungal communities of wheat (<em>Triticum aestivum</em> L.) cultivars Gladius (low N-use efficiency) and Mace (high N-use efficiency). Plants were grown until the mid-anthesis stage in a Dermosol soil treated with either 0 (Low-N) or 90 kg N ha⁻¹ (High-N). The rhizosphere fungal communities were characterised using quantitative PCR, ITS rRNA metabarcoding, and metagenomics. The abundance and diversity of the rhizosphere fungal community were not significantly influenced by N deficiency in either Mace or Gladius cultivars (<em>P</em> &gt; 0.05). However, the fungal community composition showed significant variation across N treatments in Mace (<em>P</em> &lt; 0.05), whereas no such effect was observed in Gladius (<em>P</em> &gt; 0.05). Differential abundance analysis and fungal trait predictions indicated a reduction in fungal symbionts in both cultivars under N deficiency (<em>P</em> &lt; 0.05). Metagenomic analysis demonstrated that fungal functional profiles remained unaffected by N deficiency (<em>P</em> &gt; 0.05) but significantly differed between Mace and Gladius (<em>P</em> &lt; 0.05). This study reveals intraspecific variation in rhizosphere fungal responses to N deficiency between Mace and Gladius. The metabarcoding and metagenomic data suggest functional redundancy within the fungal community, which may enhance wheat resilience under N-deficient conditions. These findings highlight the potential of using fungal community stability in developing biofertiliser products for sustainable agriculture.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101030"},"PeriodicalIF":3.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the influence of antagonistic fungi Polydomus karssenii and Niesslia gamsii on the penetration of Heterodera filipjevi and Pratylenchus thornei in wheat","authors":"Osameh Atiya ,&nbsp;Samad Ashrafi ,&nbsp;Abdelfattah A. Dababat ,&nbsp;Wolfgang Maier ,&nbsp;Halil Toktay","doi":"10.1016/j.rhisph.2025.101045","DOIUrl":"10.1016/j.rhisph.2025.101045","url":null,"abstract":"<div><div>Plant parasitic nematodes (PPNs) significantly impact global wheat production. Strategies for managing PPN include developing tolerance/resistance varieties, crop rotation, and the use of nematicides. However, nematicides are highly toxic and have a non-target effect on the soil biota, emphasizing the need for safer alternatives and innovative approaches. This study investigated the suppressive effect of two nematode egg parasitic fungi, <em>Polydomus karssenii</em> (Ashrafi et al., 2023) and <em>Niesslia gamsii</em> (Ashrafi and Maier, 2017), on the penetration of the cereal cyst nematode (CCN) <em>Heterodera filipjevi</em> (Madzhidov, 1981) and the root lesion nematode (RLN) <em>Pratylenchus thornei</em> (Sher and Allen, 1953) into wheat roots. Two nematode-susceptible wheat cultivars, Bezostaya and Seri, were treated with <em>P. karssenii</em> and <em>N. gamsii</em>. Germinated seeds were dipped in a fungal suspension before being inoculated with the nematode species. Nematode penetration was evaluated at 48 h, 72 h, and 1 week after inoculation using acid fuchsin staining and microscopical examination. The results revealed that both fungal treatments reduced nematode penetration in both cultivars compared to untreated controls, with <em>P. karssenii</em> showing higher overall effectiveness. <em>Polydomus karssenii</em> reduced penetration of <em>H. filipjevi</em> by 91.9% and <em>P. thornei</em> by 85.9%, while <em>N. gamsii</em> reduced penetration of <em>H. filipjevi</em> by 76% and <em>P. thornei</em> by 67.3%. Additionally, fungal treatments delayed nematode penetration, no penetration was observed at 48 h in treated plants, while nematode penetration was observed in untreated plants during the same period. This study underscores the potential of <em>P. karssenii</em> and <em>N. gamsii</em> as sustainable biological agents against nematodes and emphasizes their role in integrated pest management strategies for wheat production.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101045"},"PeriodicalIF":3.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disruption of the endogenous indole glucosinolate pathway impacts the Arabidopsis thaliana root exudation profile and rhizobacterial community","authors":"Daniel Acuña ,&nbsp;Molly C. Bletz ,&nbsp;Joelle Sasse ,&nbsp;Shirley A. Micallef ,&nbsp;Suzanne Kosina ,&nbsp;Benjamin P. Bowen ,&nbsp;Trent R. Northen ,&nbsp;Adán Colón-Carmona","doi":"10.1016/j.rhisph.2025.101046","DOIUrl":"10.1016/j.rhisph.2025.101046","url":null,"abstract":"<div><div>Root exudates are composed of primary and secondary metabolites known to modulate the rhizosphere microbiota. Glucosinolates are defense compounds present in the Brassicaceae family capable of deterring pathogens, herbivores and biotic stressors in the phyllosphere. In addition, traces of glucosinolates and their hydrolyzed byproducts have been found in the soil, suggesting that these secondary metabolites could play a role in the modulation and establishment of the rhizosphere microbial community associated with this family. We used <em>Arabidopsis thaliana</em> mutant lines, including the <em>cyp79B2cyp79B3</em> double mutant line with a disruption in the indole glucosinolate pathway and <em>atr1D</em>, which overexpresses ATR1 and increases glucosinolate production. These lines were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and 16S rRNA amplicon sequencing to evaluate how genetic modifications to the indole glucosinolate pathway affects the root exudate profile of <em>Arabidopsis thaliana</em>, and, in turn, impacts the rhizosphere microbial community. Metabolic analysis of root exudates from the wild-type Columbia (Col-0), along with the mutant lines, confirmed that alterations to the indole glucosinolate biosynthetic pathway result in shifts in the root exudate profile of the plant. We observed changes in the relative abundance of exuded metabolites. Moreover, 16S rRNA amplicon sequencing results provided evidence that the rhizobacterial communities associated with the plant lines used were directly impacted in diversity and community composition. This work provides further information on the involvement of secondary metabolites and their role in modulating the rhizobacterial community. Root metabolites dictate the presence of different bacterial species, including plant growth-promoting rhizobacteria (PGPR). Our results suggest that genetic alterations in the indole glucosinolate pathway cause disruptions beyond the endogenous levels of the plant, significantly changing the abundance and presence of different metabolites in the root exudates of the plants as well as the microbial rhizosphere community.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101046"},"PeriodicalIF":3.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Manipulation of root-associated bacterial endophytes for sustainable crop production system: A review","authors":"Maqsood Ahmed Khaskheli ,&nbsp;Mir Muhammad Nizamani ,&nbsp;Entaj Tarafder ,&nbsp;Diptosh Das ,&nbsp;Ghulam Muhae-Ud-Din ,&nbsp;Raheel Ahmed Khaskheli ,&nbsp;Yong Wang","doi":"10.1016/j.rhisph.2025.101044","DOIUrl":"10.1016/j.rhisph.2025.101044","url":null,"abstract":"<div><div>Manipulating endophytic communities offers a promising strategy for enhancing plant growth, stress tolerance, and disease resistance, significantly contributing to sustainable agriculture. Endophytes improve nutrient acquisition, produce growth-promoting hormones, and enhance plant defenses against various stresses and pathogens. Techniques such as specific endophyte inoculation, bio stimulant application, and genetic engineering with CRISPR/Cas9 can optimize these benefits. However, challenges exist, including maintaining specific endophytic communities, understanding their long-term impacts, and ensuring regulatory and safety compliance. High-throughput technologies, such as genomics, transcriptomics, and metabolomics, are crucial for advancing our understanding of endophyte-plant interactions. Integrated approaches that combine these advanced technologies with sustainable agricultural practices, like crop rotation and intercropping, can optimize the benefits of endophytes while mitigating potential risks. Long-term studies and robust regulatory frameworks are necessary to ensure environmental and consumer safety. By addressing these challenges and leveraging advanced scientific tools, the full potential of endophytes in creating resilient and productive agricultural systems can be realized, fostering a more sustainable and efficient approach to farming.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101044"},"PeriodicalIF":3.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143437731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing the role of rhizo-bacteria to mitigate salinity stress in rice (Orzya sativa); focus on antioxidant defense system, photosynthesis response, and rhizosphere microbial diversity","authors":"Zejian Chen ,&nbsp;Peng Zhang ,&nbsp;Bin Wang ,&nbsp;Hui Li ,&nbsp;Shuxin Li ,&nbsp;Hua Zhang ,&nbsp;Fasih Ullah Haider ,&nbsp;Xiangnan Li","doi":"10.1016/j.rhisph.2025.101043","DOIUrl":"10.1016/j.rhisph.2025.101043","url":null,"abstract":"<div><div>Salt stress threatens global food security, and although plant growth-promoting rhizobacteria (PGPR) can boost plant resistance and productivity, their field effects are poorly understood. Therefore, this experimental trial explored the mechanisms of PGPR-induced salt stress resistance on ion homeostasis, the photosynthetic system, enzymatic activities, and rhizosphere diversity in rice. The study was conducted in the first week of May 2022, using rice (Tongxi 945) seeds, which were pelleted at the seedling nursery and cultivated in the field under salinity conditions (0.5 and 2.35 g kg⁻¹) with (+) or without (−) PGPR treatment. Na⁺/K⁺ concentrations, photosynthetic, leaf water potential, enzymatic activities, and changes in rhizosphere microorganisms were measured at the heading stage of rice. The findings of this study revealed that salinity stress significantly increased Na⁺ concentrations in leaves (257.70%), the leaf Na⁺/K⁺ ratio (567.96%), and leaf water potential (63.47%) while markedly reducing the net photosynthetic rate (71.72%), stomatal conductance (81.36%), thousand-grain weight (2.22%), and yield (114.15%). However, the application of PGPR mitigated the adverse effects of salinity stress by reducing Na⁺ concentrations in roots (45.22%) and leaves (26.20%), the root Na⁺/K⁺ ratio (64.68%), and leaf water potential (31.39%). PGPR also significantly improved the net photosynthetic rate (29.75%), stomatal conductance (46.89%), transpiration rate (25.56%), and chlorophyll content (11.95%). Applying PGPR significantly enhanced antioxidant enzyme activity, regulated carbon metabolism, increased microbial diversity in rhizosphere soil, and boosted the abundance of dominant fungal genera, alleviating salt stress damage to rice. Overall, PGPR improves microbial diversity, photosynthesis, and enzyme activities, mitigating salt stress effects. Further research is necessary to implement these findings in agriculture and evaluate their long-term impacts on crop productivity and soil health.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101043"},"PeriodicalIF":3.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential rhizosphere soil nutrient use strategy of invasive and native shrub species in oak, pine, and oak-pine mixed forest ecosystems of the Himalaya","authors":"Priya Hansda,&nbsp;Shailendra Kumar,&nbsp;Satish Chandra Garkoti","doi":"10.1016/j.rhisph.2025.101021","DOIUrl":"10.1016/j.rhisph.2025.101021","url":null,"abstract":"<div><div>Vegetation types contribute differently to the soil nutrient status; however, this study aims to investigate the rhizosphere soil nutrient use strategy of invasive (<em>Ageratina adenophora</em>) and native (<em>Berberis asiatica</em> and <em>Rubus ellipticus</em>) shrub species under varied soil nutrient regimes. The rhizosphere soil samples of <em>A. adenophora</em>, <em>B. asiatica</em>, and <em>R. ellipticus</em> were collected from the upper (0–10 cm) and lower (10–20 cm) soil depths in oak, pine, and oak-pine mixed forest stands. Bulk soil was collected as control in the selected forest stands. Linear Mixed Model (LMM) and analysis of variance demonstrated that most soil properties were significantly (<em>p</em> &lt; 0.05) higher in the rhizosphere soil of <em>A. adenophora</em> than in that of <em>B. asiatica</em> and <em>R. ellipticus</em>. Specifically, soil organic carbon (SOC), total nitrogen (TN), microbial biomass carbon (MBC), nitrogen (MBN), and dehydrogenase enzyme activity (DHA) were higher in the rhizosphere of invasive <em>A. adenophora</em> than in that of native <em>B. asiatica</em> and <em>R. ellipticus</em> in oak forest. In contrast, the rhizosphere soil of native shrubs retained higher TN, Available phosphorus, and DHA than the invasive <em>A. adenophora</em> in the pine forest stand. Redundancy analysis (RDA) demonstrated that the shrub species and forest stands accounted for 22.6% and 10% variance in the rhizosphere soil properties, respectively. Among the forest stands, the rhizosphere soil traits of <em>A. adenophora</em> were higher than the rhizosphere of native species in oak forests suggesting that nutrient-rich soil ecosystems and favourable microclimatic conditions are suited for the growth and survival of invasive species. Conversely, pine forests, which are adapted to resource co-limitation and higher temperatures may suppress the proliferation of <em>A. adenophora,</em> obligating <em>A. adenophora</em> to alter its nutrient use strategies from nutrient acquisition in oak to nutrient conservation in pine forests. Thus, our findings suggest that mixed forests (oak and pine) should be conserved to enhance the species richness.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101021"},"PeriodicalIF":3.4,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143386536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus-derived cyclic lipopeptides modulate microbiome structure and enzymatic activity of soil and increase productivity of potato (Solanum tuberosum L.)","authors":"Alexey S. Vasilchenko ,&nbsp;Diana S. Dilbaryan ,&nbsp;Darya V. Poshvina ,&nbsp;Eugene O. Burlakov ,&nbsp;Olga V. Domanskaya ,&nbsp;Aleksandr V. Iashnikov ,&nbsp;Irina V. Palamarchuk ,&nbsp;Anastasia V. Teslya","doi":"10.1016/j.rhisph.2025.101033","DOIUrl":"10.1016/j.rhisph.2025.101033","url":null,"abstract":"<div><div>Cyclic lipopeptides (CLPs) are well-known secondary metabolites produced by <em>Bacillus</em> bacteria. Current research into the role of lipopeptides in soil microbial ecology suggests that their importance goes beyond the suppression of phytopathogens. Here we studied CLPs (bacillomycins and fengycins) as potential modulators of the structural (taxonomic) and functional (enzymatic activity) properties of the microbiome in agroecosystems with the aim of improving soil health and, consequently, plant productivity. The metabolic activity of the soil microbial communities has been found to be stimulated by the application of CLPs to soil. Microbial enzymes involved in the cycling of carbon (3 enzymes), nitrogen (2 enzymes) and phosphorus (1 enzyme) were activated in an experimental condition. Exposure to CLPs did not alter the alpha diversity of bacteria, but increased the alpha diversity of fungi. Amplicon sequencing showed that the action of CLPs alters the taxonomicstructure of bacterial and fungal communities. The constructed network of relationships between enzyme activity and changes in the microbial community allows us to identify potential taxa of bacteria and fungi that determine the activity of specific enzymes. The proposed mechanism behind changes in soil functional activity involves a change in the abundance of specific groups of bacteria and fungi, which gaine a competitive advantage after the introduction of CLPs into the soil. Finally, we tested the CLPs-based preparation on potato tubers and found that it improved the physiological parameters of the plants. Thus, the use of purified <em>Bacillus-</em>derived CLPs allowed better characterization of the biological effects exerted by soil bacilli on the soil microbiome. <em>Bacillus</em> CLPs are found to be non-toxic and to stimulate soil microbiota.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101033"},"PeriodicalIF":3.4,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}