Rhizosphere最新文献

{"title":"Sugarcane exudates as nitrification inhibitors; improvement of soybean nitrogen recovery and yield by reducing soil nitrification and N2O emission using 15N tracing techniques","authors":"Nipon Mawan ,&nbsp;Wanwipa Kaewpradit","doi":"10.1016/j.rhisph.2024.100871","DOIUrl":"https://doi.org/10.1016/j.rhisph.2024.100871","url":null,"abstract":"<div><p>BNI (biological nitrification inhibition) derived from root exudates is typically used in rotation cropping systems to increase the crop nitrogen efficiency (NUE). Recently, the root exudates secreted by three sugarcane varieties in Thailand have been found to inhibit the nitrification and accumulation. The objective of the study was to evaluate the effectiveness of the sugarcane root exudate on (i) soil nitrification rate and N dynamics (soil mineral N and nitrous oxide (N₂O) emission) and (ii) growth, yield, and ¹⁵N recovery by soybean under greenhouse conditions. The experiment was laid out as a randomized complete block design (RCBD), with five treatments and four replications: (i) N fertilizer (Control); (ii) Control + Uthong 13 sugarcane root exudate (UT13); (iii) Control + Khon Kaen 3 sugarcane root exudate (KK3); (iv) Control + wild cane sugarcane root exudate (Spone) and (v) Control + dicyandiamide (DCD). ¹⁵N fertilizer was applied to a micro-plot for ¹⁵N recovery. Soybean was planted and fertilized at 75 kg ha⁻¹ N as (NH₄)₂SO₄. Soil samples were collected at 1, 3, 7, 14, 21 and 28 days after N fertilization (DANF) for pH, potential nitrification rate (PNR), NH₄⁺, NO₃⁻ and MBN measurement at 0–15 and 15–30 cm soil depths. A gas sample was collected by the soil chamber technique for an N₂O emissions study on the soil sampling date. The soybean growth was monitored at 30 and 60 DAP (days after planting) for height, leaf area, leaf area index (LAI), SCMR and dry weight (DW). At soybean harvest, seed yield, yield components and N uptake were recorded. Our study revealed that the sugarcane root exudates significantly decreased PNR up to 14 DANF and increased NH₄⁺ content in soil and reduced commutative N₂O–N fluxes after 14 DANF, compared to N treatment (p &lt; 005). The root exudate treatments not only increased growth (total DW and LAI at 60 DAP), yield (number of pods and seeds per plant) and N uptake (17–19%) but also ¹⁵N recovery in the total plant. This confirms the capacity of BNIs from sugarcane root exudates. In conclusion, sugarcane root exudates can effectively mitigate N₂O emissions and enhance soybean N uptake under greenhouse conditions.</p></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139985615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understory shrub root systems and their exudates improve soil biochemistry in pine stands in temperate climate","authors":"Jarosław Lasota,&nbsp;Marzena Kaźmierczak,&nbsp;Ewa Błońska","doi":"10.1016/j.rhisph.2024.100868","DOIUrl":"https://doi.org/10.1016/j.rhisph.2024.100868","url":null,"abstract":"<div><p>The aim of our research was to determine how the addition of selected shrub species in single-species pine stands affects soil properties. We assumed that shrubs through roots and their exudates have a significant impact on the physicochemical and biological properties of soils. In our research, we took into account four study plots variants: a single-species pine stand, a pine stand with an admixture of rowan, a pine stand with an admixture of alder buckthorn and pine stand with an admixture of European hazelnut. In our research, we focused on determining the role of the root systems of selected shrub species in shaping soil properties. We determined the morphological features of the roots, their increment as well as we analyzed the amount of carbon released with their exudates. We used extracellular and intracellular enzyme activity and microbial biomass to assess biochemical status of soil. Our research indicates the significant impact of the morphological features of roots and their exudates on shaping the enzymatic activity of the tested soils. Multiple regression analysis indicates the importance of root increase, SRA and the number of tips in shaping enzymatic activity. At the same time the strong correlations were noted between the activity of extracellular and intracellular enzymes and root exudates. European hazelnut secreted the most C regardless of the sampling date. Shrubs in pine monocultures create fertile islands, determined by more favourable soil properties, which is consequently important for soil health. Our results indicate that shrubs can be valuable element of the forest ecosystem, which has a positive effect on soil properties, and as a consequence which may improve the stability of forest stands.</p></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2452219824000211/pdfft?md5=8e714a4f017f78b6cab1a3085c2b4eaf&pid=1-s2.0-S2452219824000211-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139748317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin mitigation of herbicide-induced injury to guar crop improves nodulation","authors":"Akbar Aliverdi,&nbsp;Yalda Khorshidvand","doi":"10.1016/j.rhisph.2024.100866","DOIUrl":"10.1016/j.rhisph.2024.100866","url":null,"abstract":"<div><p>The guar plant, or cluster bean, forms a weak symbiosis with rhizobia, and it is sensitive to herbicides. We hypothesized that application of melatonin to guar seeds, leaves, or both protect against metabolic injury from eight herbicides, and strengthening the symbiosis. Imazethapyr and 2,4-DB did not injure guar and its rhizobium partner. Root nodulation was severely damaged with pendimethalin and less with bentazon. A reduction in guar's total dry weight, which ranged from 14% with bentazon to 28% with bromoxynil, was observed, indicating metabolic stress. When no herbicide was applied, seeds coated with melatonin had no effect. When herbicides were applied, it partially mitigated herbicide-induced injury. Foliar application of melatonin protected guar completely against bentazon, bromoxynil, and oxyfluorfen, protected nodulation.</p></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139827795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Common Bacillus mitigate tomato verticillium wilt and bacterial specks when combined with an essential oil extract","authors":"Imane Es-sahm ,&nbsp;Siham Esserti ,&nbsp;Jamila Dich ,&nbsp;Amal Smaili ,&nbsp;Lalla Aicha Rifai ,&nbsp;Lydia Faize ,&nbsp;Tayeb Koussa ,&nbsp;Jean Stéphane Venisse ,&nbsp;Yousra Benyahia ,&nbsp;Naima Sawadi ,&nbsp;Halima Rabib ,&nbsp;Wadi Badri ,&nbsp;Mohamed Faize","doi":"10.1016/j.rhisph.2024.100865","DOIUrl":"https://doi.org/10.1016/j.rhisph.2024.100865","url":null,"abstract":"<div><p>This study was conducted to evaluate the effect of growth promoting rhizobacteria (PGPR) alone and in combination with an essential oil (EO) extracted from <em>Tetraclinis articulata</em> (Vahl) Masters in protecting tomato plants against verticillium wilt and bacterial speck diseases. Three strains, identified as <em>Bacillus toyonensis EI</em>, <em>Bacillus thuringiensi</em>s <em>ES</em> and <em>Bacillus thuringiensi</em>s <em>RA,</em> possess <em>in vitro</em> PGPR activity such as the production of indole acetic acid and ammonia, and the solubilization of phosphates. <em>In planta</em>, they significantly increased the stem length, number of leaflets, leaf area, and root biomass. EO and the three strains, alone or in combination, were able to enhance photosynthetic parameters such as net photosynthesis and transpiration rate. They also significantly reduced leaf alteration (LA) index, stunting index (SI) and browning index (BI) caused by <em>Verticillium dahliae</em> kleb. In tomato. Reduction of LAI ranged from 25% to 37%, and it enhanced to more than 60% when plants were treated with EO combined with <em>B. thuringiensi</em>s <em>SE</em> or with <em>B. toyonensis EI</em>, indicating a synergistic effect. Moreover, EO as well as the three <em>Bacillus</em> strains were also able to enhance tomato resistance against bacterial speck disease caused by <em>Pseudomonas syringae</em> pv. <em>tomato</em>. The average number of lesions was significantly reduced by the application of EO and the three strains, reaching 94% with combination of EO with <em>B. thuringiensi</em>s <em>RA</em>. Bacterial population analyses showed that plants with reduced symptoms exhibited significantly lower bacterial concentrations. In tomato plants inoculated with <em>V. dahliae</em> results showed that EO as well as three strains were able to enhance H₂O₂ accumulation, at the late stage of infection, which might lead to reduce disease. However, within the pathosystem tomato/<em>P. syringe</em> pv. <em>tomato,</em> H₂O₂ accumulation was highly enhanced by the pathogen and inhibited after combined treatment.</p></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139744172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The unique rhizosphere of aerial roots","authors":"Meisam Nazari","doi":"10.1016/j.rhisph.2024.100867","DOIUrl":"https://doi.org/10.1016/j.rhisph.2024.100867","url":null,"abstract":"","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S245221982400020X/pdfft?md5=40c29a6a331c9f490d9b1913419f0a65&pid=1-s2.0-S245221982400020X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139719068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High salinity suppresses nitrogen cycle genes and shifts nitrifier communities in the black mangrove rhizosphere","authors":"Ingrid Figueroa-Galvis ,&nbsp;Orson Mestanza ,&nbsp;Andrea Muñoz ,&nbsp;Victor A. Ramos-Duarte ,&nbsp;Javier Vanegas","doi":"10.1016/j.rhisph.2023.100846","DOIUrl":"10.1016/j.rhisph.2023.100846","url":null,"abstract":"<div><p>High salinity inhibits the nitrogen cycle, which is crucial to biogeochemical changes in coastal mangrove ecosystems. We examined <em>Avicennia germinans</em> rhizosphere soil over a salinity gradient (electrical conductivities of 5.27 mS cm⁻¹–38.64 mS cm⁻¹) to see how high salinity affects the bacterial community and metabolic nitrogen activities. Amplicon sequencing of the 16S rRNA gene examined the bacterial population profile, whereas full shotgun metagenome sequencing assessed functional genetic potential. <em>Bacillus</em>, <em>Desulfuromonas</em>, <em>Methyloceanibacter</em>, and <em>Nitrospira</em> dominated the genera, whereas <em>Proteobacteria</em>, <em>Actinobacteria</em>, and <em>Bacteroidetes</em> dominated the phyla. <em>Nitrospirae</em> dominated at high salinity. High soil salinity suppressed nitrogen cycle gene abundances: <em>nifH</em>, <em>nxrAB</em>, <em>nirS</em>, <em>nirK</em>, <em>norB</em>, <em>nirB</em>, and <em>nirA</em>. Ammonia-oxidizing bacteria like <em>Nitrosococcus</em> and <em>Nitrosomonas</em> decreased with salinity in the nitrifier population discovered by amplicon sequencing. Nitrite-oxidizing bacteria like <em>Nitrospira</em> and <em>Nitrospina</em> rose at high salinity, whereas <em>Nitrococcus</em> and <em>Nitrolancea</em> declined. Salinity reduces nitrogen gene abundances in most nitrifier community members, inhibiting the nitrogen cycle.</p></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139667659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro callus induction and growth for medicinal use of an endangered Korean native plant, Glochidion chodoense C. S. Lee & H. T. Im","authors":"Jin-Ho Kim,&nbsp;Hae Lim Kim,&nbsp;Sea-Hee Han,&nbsp;Hwa Lee,&nbsp;Jong-Eun Han,&nbsp;Tea-Su Kim,&nbsp;Su Hui Seong,&nbsp;Bo-Ram Kim,&nbsp;Ha-Nul Lee,&nbsp;Chan Seo,&nbsp;Jung Eun Kim,&nbsp;Sua Im,&nbsp;Ji Min Jung,&nbsp;Kyung-Min Choi,&nbsp;Jin-Woo Jeong","doi":"10.1016/j.rhisph.2024.100863","DOIUrl":"10.1016/j.rhisph.2024.100863","url":null,"abstract":"<div><p><em>Glochidion chodoense</em> C. S. Lee &amp; H. T. Im is an endangered plant found in Korea. Its conservation and potential industrial utilization require the development of suitable genetic resource propagation approaches. We here present a protocol for the efficient propagation of <em>G. chodoense</em><span> leaf segments by callus induction. We assessed various induction media containing different concentrations of plant growth regulators, as well as antioxidants to inhibit callus browning. The use of MS medium with 2,4-Di-chlorophenoxy-acetic acid (2,4-D) (1 mg/L) and BA (0.5 mg/L) for callus induction and incorporating ascorbic acid (10 mg/L) to mitigate browning was found to offer a promising approach for the mass production of </span><em>G. chodoense</em><span>. This protocol has significant potential for the conservation and industrial utilization of this endangered species.</span></p></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139667636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"2-D soil zymography: Accounting for the spatial variation of pH","authors":"Andrey Guber,&nbsp;Alexandra Kravchenko","doi":"10.1016/j.rhisph.2024.100862","DOIUrl":"10.1016/j.rhisph.2024.100862","url":null,"abstract":"<div><p><span>Soil zymography is commonly used to quantify spatial distribution of hydrolytic </span>enzyme activities<span> on soil and plant root surfaces. It is recommended to adjust pH in zymography substrates and calibration solutions with respect to soil/root pH. However, pH values may vary greatly within a few mm of plant rhizosphere, potentially altering the distribution of pH in zymography membranes. Despite the fact that the effect of pH on the calibration of zymography membranes is generally known, its potential impact on zymography results is unaccounted for in processing zymography images and calculations of enzyme activity. In this study we assessed the effect of pH variations on the persistency of the methylumbelliferone (MUF) calibration. The studied pH values ranged from 4.5 to 7.5. The MUF calibration curves greatly deviated from that at a reference pH of 6.5, with a marked nonlinear increase of deviation with greater membrane brightness. We suggest that the problem can be partially alleviated by reducing the membrane incubation time. However, such deviations suggest the need for a more comprehensive resolution via mapping pH and using pH-specific calibrations to process zymography images. We developed a MATLAB code to implement a pixel-based correction of enzyme activity for pH in processing time-lapse zymography images.</span></p></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139667657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"External phosphorus enhances the efficient acquisition of phosphorus by alfalfa (Medicago sativa L.) through its interaction with root morphological traits and rhizosphere carboxylates","authors":"Tengyue Du ,&nbsp;Shaoting Zhao ,&nbsp;Xiaoqin Zheng ,&nbsp;Xiaofan Ding ,&nbsp;Huan He ,&nbsp;Gang Xie ,&nbsp;Junhao Zhu ,&nbsp;Yuyang Kou ,&nbsp;Weibo Shen","doi":"10.1016/j.rhisph.2024.100861","DOIUrl":"10.1016/j.rhisph.2024.100861","url":null,"abstract":"<div><p><span><span>Root morphological characteristics and rhizosphere carboxylates are the critical factors for phosphorus (P) absorption in </span>forage crops such as alfalfa (</span><span><em>Medicago sativa</em></span> L.) include root morphological characteristics and rhizosphere carboxylates. This study explored alfalfa P uptake across various P application levels and evaluated the respective contributions of root morphological traits and rhizosphere carboxylates to this process. We analyzed root morphological characteristics and composition and amounts of rhizosphere carboxylates and their contribution in P uptake after cultivating alfalfa in pots with seven distinct P application levels for 9 weeks. The results revealed that supplying P enhanced the growth of aboveground and underground plant parts. At a P application level of 12 mg kg⁻¹<span>, alfalfa stems exhibited the highest concentration and uptake of P. Concurrently, root parameters such as root length (RL), root surface area (RSA), root volume, root tips and the amount of rhizosphere carboxylates were the highest. Although some P was allocated to aboveground parts, root exposure to higher P supply levels (48 and 96 mg kg</span>⁻¹<span>) can lead to P toxicity and reduce the P absorption. Structural equation modeling (SEM) and regression analysis demonstrated that RSA, root tips and rhizosphere carboxylate content significantly affected alfalfa P absorption. Among them, malate was crucial for promoting P uptake in alfalfa under high P concentrations. In summary, this study elucidated the dominant factors affecting plant P absorption and identified the optimal concentration (12 mg kg</span>⁻¹<span>) for alfalfa growth by examining the response of alfalfa to varying P concentrations. Additionally, this study also provides a foundation for the development of low-P agriculture in artificial alfalfa plantations.</span></p></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139667640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical diversity of crop root mucilages: Implications for their maximal water content and decomposition","authors":"S. Le Gall ,&nbsp;C. Lapie ,&nbsp;F. Cajot ,&nbsp;C. Doussan ,&nbsp;L. Corridor ,&nbsp;A. Bérard","doi":"10.1016/j.rhisph.2024.100858","DOIUrl":"10.1016/j.rhisph.2024.100858","url":null,"abstract":"<div><p><span><span>The biophysical functioning of the rhizosphere can act on plant </span>water use efficiency through root mucilage release and their stimulation of microorganisms' activity. Mucilage characteristics and roles are still poorly studied for the diversity of agronomic plant species. We compared mucilages collected from roots of germinated seeds (aerohydroponics technique) of eight plant species (four </span>Eudicotyledons<span>, four Monocotyledon) in terms of total sugars content, Medium Infrared (MIR) spectra, mucilage maximal water content and induced respiration in contrasting soils. Our results suggest that the “chemical fingerprint” of these mucilages could be discriminated according to the phylogenetic proximity of the plant species. In addition, the maximal water content that mucilage retain seems to be linked to their chemical composition and seems more related to the presence of high molecular weight sugars than their total sugar amounts. If mucilage-induced respiration by soil microbiota appeared to be independent from phylogeny, some mucilages induced more respiration than others regardless of the soil studied. Microbial communities and soil physico-chemical properties interact in decomposition with variations in mucilage's chemical composition.</span></p></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139668019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}