Rhizosphere最新文献

{"title":"Eggplant rootstocks enhance flooding tolerance in grafted tomato compared to wild tomato rootstocks","authors":"Priti K. Mote ,&nbsp;Pratapsingh S. Khapte ,&nbsp;Bharati B. Misal ,&nbsp;Shruti S. Sarode ,&nbsp;Machindra G. Agale ,&nbsp;Ganesh S. Shinde ,&nbsp;Sushil S. Changan ,&nbsp;Vanita N. Salunkhe ,&nbsp;K. Sammi Reddy","doi":"10.1016/j.rhisph.2025.101194","DOIUrl":"10.1016/j.rhisph.2025.101194","url":null,"abstract":"<div><div>Flooding, caused by intense rainfall during the rainy season, severely limits tomato productivity. Grafting tomato onto eggplant or wild tomato species can enhance tolerance by combining genetically diverse root systems with high-yielding scion. The tomato (<em>Solanum lycopersicum</em>) hybrid ‘Aryaman’ was grafted onto thirteen rootstocks from eggplant (<em>S. melongena</em>) and wild tomato species and evaluated under control and ten-day flooding conditions. Significant variation was observed among grafts for growth, physiological, biochemical, fruit quality and yield traits. Eggplant rootstock grafts <em>S.ly/S.me2</em> and <em>S.ly/S.me3</em> produced yields two-fold higher under flooding compared to non-grafted tomato, with survival rates of 30 % and 20 %, respectively. In contrast, wild tomato rootstock grafts and non-grafted plants showed complete mortality, resulting in a 90 % yield reduction. The enhanced tolerance of eggplant rootstock grafts is attributed to higher SPAD index, reduced oxidative damage, better plant water status, sustained photosystem II efficiency, and greater root biomass. These results demonstrate that eggplant rootstocks <em>S.me2</em> and <em>S.me3</em> mitigate flooding stress through root-mediated mechanisms, enhancing physiological resilience and maintaining tomato yield.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"36 ","pages":"Article 101194"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145159120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Key bacterial players in the growth of Kandelia obovata: Insights from rhizosphere soil composition","authors":"Shouji Gong ,&nbsp;Xiaokui Xie ,&nbsp;Riming Wang ,&nbsp;Xiujian Li","doi":"10.1016/j.rhisph.2025.101196","DOIUrl":"10.1016/j.rhisph.2025.101196","url":null,"abstract":"<div><div>The structure of soil bacterial communities within mangrove forests has received widespread attention, which can promote the growth of mangroves and material transformation. Despite the recognized significance of mangrove ecosystems, the bacterial composition within artificially restored mangroves remains poorly understood. This study investigates the bacterial diversity in the rhizosphere soil of both naturally and artificially restored <em>Kandelia obovata</em> seedlings, utilizing Illumina NovaSeq high-throughput sequencing technologies. The results revealed that only a small fraction of bacteria were identified in the mangrove rhizosphere soil, with the majority of them remaining uncharacterized. The dominant bacterial taxa identified included <em>Sulfurovum</em>, <em>Actibacter</em>, <em>Woeseia</em>, <em>Desulfatiglans</em>, <em>Halioglobus</em>, <em>Ignavibacterium</em>, <em>Spirochaeta</em>, <em>Sulfurimonas, Prolixibacter</em>, <em>Robiginitalea</em>, and <em>Algoriphagus</em>. Furthermore, marked differences were noted in the abundance of <em>Sulfurovum</em>, <em>Actibacter</em>, <em>Woeseia</em>, <em>Desulfatiglans</em>, <em>Halioglobus</em>, <em>Methanosaeta</em> and <em>Robiginitalea</em> between natural and artificially restored <em>Kandelia obovata</em> seedlings. Spatial network analysis suggested that <em>Sulfurovum</em>, <em>Actibacter</em>, <em>Ignavibacterium</em>, and <em>Desulfatiglans</em> may play important roles in the growth process of <em>Kandelia obovata</em> and are potentially important bacteria for its development. These findlings enhance our understanding of bacterial community structure in mangroves and underscore the potential application of bacteria in mangrove restoration.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"36 ","pages":"Article 101196"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145267533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potassium salt of 1-naphthaleneacetic acid promotes rhizogenesis in Amsonia elliptica stem cuttings","authors":"Youn Hwa Son ,&nbsp;Suejin Park ,&nbsp;Chung Ho Ko ,&nbsp;Mengmeng Gu ,&nbsp;Seung Youn Lee","doi":"10.1016/j.rhisph.2025.101203","DOIUrl":"10.1016/j.rhisph.2025.101203","url":null,"abstract":"<div><div><em>Amsonia elliptica</em> is valued for its medicinal and ornamental potential. However, it is listed as an endangered species in South Korea due to overharvesting and habitat destruction. This study aimed to develop an efficient protocol for mass vegetative propagation of <em>A. elliptica</em> using stem cuttings. We tested Rootone (0.4 % 1-naphthylacetic acid) and potassium salts of indole-3-butyric acid (K-IBA) and 1-naphthaleneacetic acid (K-NAA) applied via basal dip or foliar spray. After six weeks, cuttings treated with 2000 mg L⁻¹ K-NAA showed the best rooting traits, with 93.3 % rooting, 17.5 roots per cutting, and 55.4 mm root length, along with the highest fresh (92.9 mg) and dry root mass (8.8 mg). Dipping cuttings in 2000 mg L⁻¹ K-NAA for 1 min is recommended as the most effective treatment for large-scale propagation of <em>A. elliptica</em>. This optimized propagation method will facilitate the conservation and ornamental use of <em>A. elliptica</em>.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"36 ","pages":"Article 101203"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145221128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tea varieties modulate rhizosphere microbiome assembly via root metabolite-mediated selection","authors":"Litao Sun ,&nbsp;Jiazhi Shen ,&nbsp;Huan Wang ,&nbsp;Yujie Song ,&nbsp;XiaoJiang Li ,&nbsp;Caihong Bi ,&nbsp;ShuangShuang Wang ,&nbsp;Xiuxiu Xu ,&nbsp;Yu Wang ,&nbsp;Zhaotang Ding","doi":"10.1016/j.rhisph.2025.101189","DOIUrl":"10.1016/j.rhisph.2025.101189","url":null,"abstract":"<div><div>Plant variety-specific belowground environment shapes rhizosphere microbial communities, yet the mechanisms underlying tea plant (<em>Camellia sinensis</em>) variety-driven microbiome assembly remain unclear. We integrated multispectral phenotyping, soil physicochemical analysis, microbiome sequencing, and root metabolomics, to compare three clonal tea varieties, Zhongcha 108 (ZC), Jinxuan (JX), and Jinmudan (JMD). Results showed that significant differences in canopy phenotypes were observed among tea varieties, as manifested by distinct spectral reflectance and vegetation indices. Microbial community structure differed significantly among all varieties at both phylum and genus levels. ZC enriched photoautotrophic taxa (e.g., <em>Cyanobacteriales</em>, <em>Acetobacteraceae</em>) and saprotrophic fungi. JX favored chemoheterotrophic bacteria (e.g., <em>Oceanobacillus</em>, <em>Brevibacillus</em>) and undefined saprotrophs. JMD enriched bacterial genera like <em>SBR1031</em> and arbuscular mycorrhizal fungi (e.g., <em>Paraglomerales</em>). Soil physicochemical properties were typically at higher levels in JX than that in ZC and JMD, and correlated negatively with bacterial phyla Verrucomicrobiota, Acidobacteriota, and Chloroflexi, and fungal phylum Glomeromycota. Root metabolites, especially amino acids, sugars, and diterpenoids, were key drivers of microbial assembly. Correlation analysis identified Acidobacteriota, Chloroflexi, and Firmicutes as key bacteria, and Zoopagomycota as key fungus linked to varietal variation of metabolite profiles. Predicted microbial functions revealed that ZC associated with photoautotrophy, JX/JMD with chemoheterotrophy and nutrient cycling. JMD specifically enriched arbuscular mycorrhizal functions. Variance partitioning analysis (VPA) revealed that the drivers of microbial assembly were kingdom-specific. Root metabolites emerged as the dominant factor explaining the variance in bacterial communities. In contrast, the variance in fungal communities was primarily explained by the shared effect of soil properties and root metabolites, indicating a synergistic interaction. Our findings reveal that root metabolites are the dominant factor underlying tea variety-driven assembly of the rhizosphere microbiome, offering microbiome-driven strategies for precision plant breeding and management.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"36 ","pages":"Article 101189"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drought-induced shifts in rhizosphere microbiota correlate with reprogramming of floral volatiles in Chrysanthemum morifolium ‘Gongju’","authors":"Zhuannan Chu ,&nbsp;Mingzhe Cheng ,&nbsp;Yuning Sun ,&nbsp;Weiwen Li ,&nbsp;Dongmei Yin","doi":"10.1016/j.rhisph.2025.101219","DOIUrl":"10.1016/j.rhisph.2025.101219","url":null,"abstract":"<div><div>Drought stress is a key environmental driver that restructures the rhizosphere microbiome, a critical interface for plant-soil-microbe interactions. This study investigates how a gradient of drought stress (control, mild, moderate, severe) alters the assembly and function of the bacterial community in the rhizosphere of the medicinal plant <em>Chrysanthemum morifolium</em> ‘Gongju No. 1’, and how these belowground microbial shifts are linked to aboveground changes in the floral volatile metabolome. Using 16S rRNA gene sequencing, we found that drought significantly increased microbial richness (Chao1, <em>P</em> &lt; 0.05) and induced a progressive community shift in community composition. The relative abundance of putative plant-beneficial phyla like <em>Actinobacteriota</em> increased, while moisture-sensitive groups like <em>Verrucomicrobiota</em> declined. Concurrently, GC-MS metabolomics revealed a reprogramming of the floral volatile profile, with 25 significantly differential metabolites identified under severe stress. Crucially, correlation analysis uncovered significant associations between specific rhizosphere taxa (e.g., <em>Chryseobacterium</em>, <em>Pseudolabrys</em>) and key volatiles (e.g., (+)-7-epi-sesquiterpene, limonene). Our findings suggest that drought stress triggers a coordinated response where the restructuring of the root-associated microbial community may play a role in modulating the host plant's secondary metabolism, highlighting a potential belowground-aboveground signaling axis. This study suggests that the drought-restructured rhizosphere microbiota, particularly the enrichment of taxa like Chryseobacterium, may play a role in modulating the host plant's secondary metabolism, highlighting a potential microbiome-mediated mechanism for plant adaptation to water deficit.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"36 ","pages":"Article 101219"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145465855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First-order lateral roots drive the highest rhizosphere acid phosphatase activity in soybean under phosphorus deficiency","authors":"Tantriani ,&nbsp;Benito Heru Purwanto ,&nbsp;Weiguo Cheng ,&nbsp;Keitaro Tawaraya","doi":"10.1016/j.rhisph.2025.101166","DOIUrl":"10.1016/j.rhisph.2025.101166","url":null,"abstract":"<div><div>Plants enhance acid phosphatase activity in the rhizosphere to adapt to phosphorus (P) deficiency. However, variation in acid phosphatase activity across the first-, second-, and third-order lateral roots in soybean cultivars under low-P conditions remains inadequately explored. We aimed to elucidate the distribution and variation of acid phosphatase activity among these root orders in soybean cultivars with differing tolerance to P deficiency. Four soybean cultivars—two low-P-tolerant (Kuromame and Daizu) and two low-P-sensitive (Natsu and Kumaji)—were grown in Andosol soil under two P treatments: 0 g P kg⁻¹ (−P) and 0.44 g P kg⁻¹ (+P) for 16 and 23 days after transplanting. Rhizosphere soil samples were collected from the first-, second-, and third-order lateral roots. P deficiency significantly reduced shoot and root dry weight of sensitive cultivars by 52–76 %, whereas tolerant cultivars maintained biomass without significant reduction. Root length of Daizu increased by 40–58 % under −P, while Natsu and Kumaji were 57–69 % shorter compared with +P. Acid phosphatase activity peaked in first-order roots, reaching 3.83 μmol p-NP g⁻¹ soil h⁻¹ in tolerant cultivar. Acid phosphatase activity of tolerant cultivars was strongly induced under −P, increasing 1.6- to 5.7-fold depending on root order. Phosphorus deficiency reduced root P concentration by 48 % and shoot P uptake by 52 % in sensitive cultivars but tolerant cultivars exhibited stable shoot P concentration and uptake. These results demonstrate the importance of root architecture and acid phosphatase activity in improving P acquisition under deficiency stress.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"36 ","pages":"Article 101166"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145050370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maize landraces under water deficit favor diverse rhizosphere communities associated with improved stress response","authors":"Giulia Castorina ,&nbsp;Alessia Follador ,&nbsp;Martina Ghidoli ,&nbsp;Patrizia Zaccheo ,&nbsp;Laura Crippa ,&nbsp;Fulvia Tambone ,&nbsp;Alessandro Passera ,&nbsp;Carlotta Balconi ,&nbsp;Gabriella Consonni ,&nbsp;Paola Casati","doi":"10.1016/j.rhisph.2025.101200","DOIUrl":"10.1016/j.rhisph.2025.101200","url":null,"abstract":"<div><div>Climate change is intensifying water scarcity, posing major challenges to global crop productivity. Improving tolerance to limited water availability is therefore a key agricultural priority. While elite genotypes are widely used in breeding, maize landraces represent an underexploited reservoir of adaptive traits. Their interaction with soil microbial communities may play an important role in stress resilience that needs further investigation to have its extent fully understood. In this study, we investigated the rhizosphere microbiota of four maize landraces from Lombardy (Northern Italy) to assess how soil origin, plant genotype, and water availability interact in shaping bacterial communities. Plants were cultivated in soils collected from four locations, first under well-watered conditions and then exposed to water deficit. Growth and photosynthetic traits were monitored in parallel to link microbial composition with plant performance. Under well-watered conditions, rhizosphere communities were strongly shaped by the soil–genotype combination, with consistent enrichment of <em>Bacillota</em> taxa. Under water deficit, however, most landraces/soil combinations exhibited a reduced rhizosphere effect that brought rhizosphere bacterial communities to become indistinguishable from bulk soil. Strikingly, landraces displaying the best tolerance to water deficit showed an increase of biodiversity in the rhizosphere bacterial community, suggesting a recruitment strategy opposing that shown in well-watered conditions. These results highlight the importance of integrating landrace diversity and microbiome interactions into strategies for improving maize resilience. The study demonstrates that not only soil and genotype, but also the capacity to sustain distinctive and diverse microbial associations under stress, may contribute to plant performance in water-limited environments.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"36 ","pages":"Article 101200"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145267531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Entrophospora etunicata enhances manganese stress tolerance in tea plants by increasing antioxidant enzyme defense and modulating quality-related metabolism","authors":"Ke-Xing Yan ,&nbsp;Ren-Hua Huang ,&nbsp;Xiu-Bing Gao ,&nbsp;Abeer Hashem ,&nbsp;Elsayed Fathi Abd_Allah ,&nbsp;Qiang-Sheng Wu ,&nbsp;Can Guo","doi":"10.1016/j.rhisph.2025.101226","DOIUrl":"10.1016/j.rhisph.2025.101226","url":null,"abstract":"<div><div>Manganese (Mn) is an essential microelement for plant metabolism, but its excess can induce oxidative stress and disrupt nutritional balance. Arbuscular mycorrhizal fungi (AMF) have been demonstrated to enhance plant adaptability under environmental stresses; however, the mechanisms by which they mitigate Mn stress in tea seedlings are not yet well understood. This study investigated the effects of the arbuscular mycorrhizal fungus <em>Entrophospora etunicata</em> on plant performance, antioxidant enzyme defense, leaf quality, and expression of genes associated with amino acid biosynthesis in tea (<em>Camellia sinensis</em> cv. 'Fuding Dabaicha') seedlings under varying Mn stress (0 mM, 10 mM, and 40 mM MnSO₄). The results revealed that appropriate Mn concentrations (10 mM) promoted plant growth and root mycorrhizal colonization, whereas excessive Mn (40 mM) distinctly inhibited plant growth and the colonization, compared with 0 mM Mn conditions. AMF inoculation significantly enhanced key growth parameters, including plant height and leaf number, across all Mn treatments. Regarding the antioxidant response, AMF inoculation demonstrated tissue-specific and concentration-dependent effects on antioxidant enzyme activities under Mn stress, as evidenced by significant increases in root catalase (CAT) and peroxidase (POD) and leaf POD activities under 10 mM Mn and root superoxide dismutase (SOD), POD and CAT and leaf CAT under 40 mM Mn, along with reduced oxidative damage (4.46–13.27 % lower malondialdehyde levels) across all Mn treatments. In terms of leaf quality, AMF inoculation significantly enhanced caffeine content across all Mn treatments and specifically increased tea polyphenol levels under 10 mM Mn conditions and total amino acids levels under 0 mM Mn conditions. AMF inoculation differentially regulation the expression of amino acid biosynthesis genes, up-regulating <em>CsHMGR</em>, <em>CsGDH</em>, <em>CsGS</em> and <em>CsGOGAT</em> expression at 0 mM and <em>CsGDH</em>, <em>CsGS</em>, and <em>CsGOGAT</em> expression at 10 mM Mn, while down-regulating <em>CsHMGR</em>, <em>CsGDH</em> and <em>CsGOGAT</em> expression at 40 mM Mn. It concludes that mycorrhizal symbiosis enhances Mn stress tolerance in tea seedlings through a coordinated improvement in growth, activation of antioxidant enzyme systems, and modulation of quality-related metabolic pathways, highlighting its potential application in sustainable tea cultivation.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"36 ","pages":"Article 101226"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145466427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial mechanisms targeting mineralization-mobilization dynamics and balance in rhizosphere: A necessity for future rhizosphere-soil health","authors":"Sudhir Kumar Upadhyay ,&nbsp;Prasann Kumar ,&nbsp;Devendra Jain ,&nbsp;Yogesh K. Ahlawat ,&nbsp;Xiuyun Zhao","doi":"10.1016/j.rhisph.2025.101181","DOIUrl":"10.1016/j.rhisph.2025.101181","url":null,"abstract":"<div><div>Maintaining the mineralization-mobilization balance in the rhizosphere is particularly important in the face of intense agriculture and climate change to solve world issues like soil degradation, poor nutrient usage efficiency, and sustainable crop production. This study investigates at redox transformation, chelation, microbial-driven enzymatic mineralization of organic matter, and mineral-bound nutrient mobilization via acidification, all of which are regulated by microbial activity. Dynamic root-microbe interactions can help to develop a distinct microbial ecology, and root exudates influence microbial gene expression, which regulates nutrient availability. Furthermore, the findings highlight how syntrophic microbial consortia, such as arbuscular mycorrhizal fungi, phosphate-solubilizing bacteria, and diazotrophs interact to promote nutrient cycling and soil resilience. Knowing the disturbances brought about by abiotic stresses such as pollution, salt, and drought, the review emphasizes, firstly, stress-induced imbalances in microbial populations and nutrient cycles as initial considerations for rectification. The anticipated trend topics map for next 100 years (2025–2125) confirms a first future focus on advanced soil technology and space-agriculture interfaces. This review discusses biotechnological advancements including microbial gene editing and bioformulations that can help restore the mineralization-mobilization balance. It fills critical knowledge gaps in rhizospheric interactions, laying the foundation for next-generation biofertilizers, improved crop resilience, and sustainable soil health.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"36 ","pages":"Article 101181"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145107509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fertilization alters soil microbiome and root metabolites in grafted mulberry (Morus alba) with varied scion-rootstock combinations","authors":"Xin Chen,&nbsp;Haowen Fang,&nbsp;Yunfei Zhang,&nbsp;Zeyu Liu,&nbsp;Yingting Qin,&nbsp;Shuhang Cai,&nbsp;Jiayi Li,&nbsp;Lifu Zheng,&nbsp;Zhiwei Hou,&nbsp;Xiling Wang","doi":"10.1016/j.rhisph.2025.101172","DOIUrl":"10.1016/j.rhisph.2025.101172","url":null,"abstract":"<div><div>Mulberry (<em>Morus alba</em>), a vital host plant for silkworms (<em>Bombyx mori</em>), is valued for its rapid growth, stress tolerance and nutritional benefits. Grafting is a key propagation method with rootstocks influencing nutrient uptake, stress responses and rhizosphere microbial communities. In this study, three mulberry scions (Wanghaiqing, Daliangshan, Yunsang) were grafted onto Guiyou62 rootstock. Agronomic traits, soil properties, rhizosphere and endophyte communities and root metabolites (untargeted metabolomics) were analyzed. The results showed significant variations in plant growth, yield and soil nutrient levels among scions. Fertilization enhanced biomass but reduced microbial diversity. Dominant bacterial genera (e.g <em>Sphingomonas</em>, <em>Rhizorhapis</em>) increased under fertilization while fungal communities were less affected. Root metabolomic analysis identified 732 metabolites with phenylpropanoids and polyketides being the most abundant. Fertilization upregulated key metabolites such as L-proline, Folinic acid linked to stress tolerance and nutrient metabolism. The distinct accumulation of tropine in DLS and trigonelline in YS suggested genotype-specific regulation of secondary metabolic pathways. These findings highlighted the interplay between scion-rootstock combinations, soil microbiota, and fertilization in shaping mulberry productivity and soil health, providing insights for optimizing cultivation practices.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"36 ","pages":"Article 101172"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145050371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}